msl-3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	msl-3
Entrez Gene ID	38779
Full Name	male-specific lethal 3
Synonyms	CG8631,DmMsl-3,Dmel\CG8631,MSL,MSL-3,MSL3,MSL3_DROME,Msl 1-3,Msl3,mle(3)132,mle3,mls3,msl,msl3
General protein information	Preferred Names male-specific lethal 3 Names CG8631 gene product from transcript CG8631-RA CG8631-PA gene 2 male sex lethal 3 male sex lethal-3 male specifc lethal male specific lethal 3 msl-3-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 65E4-65E4\|3-25.8 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_079227.3	NP_523951.1	male-specific lethal 3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Msl3	NP_034962.2
Xenopus tropicalis (tropical clawed frog)	msl3	XP_004912403.1
Homo sapiens (human)	MSL3	NP_523353.2
Rattus norvegicus (Norway rat)	Msl3	NP_001014133.1
Canis lupus familiaris (dog)	MSL3	XP_548862.2
Gallus gallus (chicken)	MSL3	XP_004938456.1
Drosophila melanogaster (fruit fly)	msl-3	NP_523951.1
Xenopus tropicalis (tropical clawed frog)	msl3	NP_001016574.1
Pan troglodytes (chimpanzee)	MSL3	XP_001144354.1
Bos taurus (cattle)	MSL3	NP_001179333.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001917	XP_321146.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MSL3 plays an important role in targeting the male specific lethal complex to chromatin in both humans and flies by binding to H4K20Me(1).
Title: Structural and biochemical studies on the chromo-barrel domain of male specific lethal 3 (MSL3) reveal a binding preference for mono- or dimethyllysine 20 on histone H4.

A DNA-dependent interaction of MSL3 chromodomain with the H4 lysine20 monomethyl mark, was discovered.
Title: Corecognition of DNA and a methylated histone tail by the MSL3 chromodomain.

Using ChIP-chip analysis, the authors find that MSL3 chromodomain mutants retain binding to chromatin entry sites but show a clear disruption in the full pattern of MSL targeting in vivo, consistent with a loss of spreading.
Title: The MSL3 chromodomain directs a key targeting step for dosage compensation of the Drosophila melanogaster X chromosome.

These results provide strong evidence for both sequence-dependent and -independent steps in MSL targeting of dosage compensation to the male X chromosome.
Title: A sequence motif within chromatin entry sites directs MSL establishment on the Drosophila X chromosome.

Results suggest that the MSL complex (including MSL-1, -2, and -3) uses high-affinity sites to initially recognize the X chromosome and then associates with many of its targets through sequence-independent features of transcribed genes.
Title: MSL complex is attracted to genes marked by H3K36 trimethylation using a sequence-independent mechanism.

The following msl-3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the msl-3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17752	NM_079227.3 Latest version!	Drosophila melanogaster male-specific lethal 3 (msl-3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

msl-3 ( NM_079227.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523951.1 Drosophila melanogaster male-specific lethal 3 (msl-3), mRNA.

Price & Availability↑

CloneID	OFa17752
Clone ID Related Accession (Same CDS sequence)	NM_079227.3
Accession Version	NM_079227.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	male-specific lethal 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079227.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGGAGC TAAGGGACGA GACACCGCTC TTTCACAAGG GCGAGATAGT CCTTTGCTAC 
GAGCCGGATA AATCGAAGGC CCGTGTTCTT TACACCAGCA AGGTGCTAAA CGTCTTTGAG 
CGCCGGAACG AGCATGGCCT GAGGTTCTAC GAGTACAAGA TTCACTTCCA GGGCTGGCGT 
CCATCCTACG ATCGCTGTGT GCGCGCCACC GTTCTATTGA AAGACACCGA GGAGAATCGC 
CAGTTGCAAA GGGAGCTGGC CGAAGCTGCC AAGCTGCAAA TTCGTGGTGA CTACTCCTAC 
AAGGGCACAC CCGATAAGCC ATCGGCCAAG AAGAAACGCG GCGGAAAAGC GGCCCACGTT 
GAGGAGCCAA TAGTGGTTCC AATGGACACG GGACATCTTG AGGCGGAGCA CGAAATGGCG 
CCCACTCCAC GAGCAGCGGG CAACCGGACG CGGGACAACA GCGGTGGCAA GCGAAAGGAA 
AAACCCCCGT CCGGAGATGG CCGATTAAAG GGAAACCGGG GCAGACAAAC AGAGACCTTT 
TATAACAATG CCATAAATGA TGTATCGGTC TATAACCATG TGCCACAGGA GGATCGCATT 
ATGATGCGAG TCAGCGAGCG ATTGCGAGAG CTTATAGAGT ACGATCGGAA TATGATCAAG 
GTACTAGGCA AACAGCACGC ACTGCCCGCC CGCGTGCCCA TCGTGACCAT CATGGAAAAC 
TTCGTCAAGC AGCAGGCCGT GGAGCTAGCC ATTAGCATTA AACAGGACAG CTCGAGGGCC 
AGGAACACGC AGAGTCGCAA TGCCAGAATG GAGCGGGAAT ACGATCGCGT TATGTCCACT 
GTCTGCATGC TGAAGGAGGT GGTAGACGGA TTGCGTATCT ACTTTGAGTT CCACGTGGAT 
GACCACTTGC TGTATACGGA GGAGAAGGAG TATGTCCACA ACTACCTCAC AGACGACAAC 
ATGCGCAACT GCAGCCTAAT ATTGAACAAG TCGTACGAGT ATATCAATCC CAGCGGTGAC 
ACGGAACTGA TCGGTCTGGA CGGCACGCCA GTGGTAGAAG GGTCGGGCGA CACCAATGGA 
CAGATCGGAG TTATTAATAT CGGCGGTCCG GAGTACGAGA AGCAGTTGCA AAAATGCCTG 
CTCTACATTG TTACCGCCAG CGGGAAAAAC ACTGCGCAGG CCTATGAGAG GACTTCCCCA 
TATACGGCTG CCTACAAACT GCCCGTGGAG ATGCGTGGAT TTCTGAACGA AACATTCAAG 
TGGCGGCTTT TGTCAGCGGA ATCGCCGCCA GAAAAGTCCA TGGTCTTTGG CGCTCCCCAC 
CTGGTTAGGC TAATGATTAA AATGCCCATG TTCTTAAACG CATCGCCGAT CAGCAACAAG 
AAGCTAGAAG ATCTACTTCC CCACCTCGAC GCCTTCATCA ACTACTTGGA GAACCACAGG 
GAGTGGTTCG ACAGGGAAAA CTTTGTTAAC TCCACTGCCC TGCCCCAGGA AGATCTGCAG 
CGGGAGCTGT TGGACTCCCT GGATGGGATT GCTGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523951.1
CDS	112..1650
Translation	MTELRDETPLFHKGEIVLCYEPDKSKARVLYTSKVLNVFERRNEHGLRFYEYKIHFQGWRPSYDRCVRATVLLKDTEENRQLQRELAEAAKLQIRGDYSYKGTPDKPSAKKKRGGKAAHVEEPIVVPMDTGHLEAEHEMAPTPRAAGNRTRDNSGGKRKEKPPSGDGRLKGNRGRQTETFYNNAINDVSVYNHVPQEDRIMMRVSERLRELIEYDRNMIKVLGKQHALPARVPIVTIMENFVKQQAVELAISIKQDSSRARNTQSRNARMEREYDRVMSTVCMLKEVVDGLRIYFEFHVDDHLLYTEEKEYVHNYLTDDNMRNCSLILNKSYEYINPSGDTELIGLDGTPVVEGSGDTNGQIGVINIGGPEYEKQLQKCLLYIVTASGKNTAQAYERTSPYTAAYKLPVEMRGFLNETFKWRLLSAESPPEKSMVFGAPHLVRLMIKMPMFLNASPISNKKLEDLLPHLDAFINYLENHREWFDRENFVNSTALPQEDLQRELLDSLDGIAA

Target ORF information:

RefSeq Version	NM_079227.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster male-specific lethal 3 (msl-3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079227.3

ORF Insert Sequence:

ATGACGGAGC TAAGGGACGA GACACCGCTC TTTCACAAGG GCGAGATAGT CCTTTGCTAC 
GAGCCGGATA AATCGAAGGC CCGTGTTCTT TACACCAGCA AGGTGCTAAA CGTCTTTGAG 
CGCCGGAACG AGCATGGCCT GAGGTTCTAC GAGTACAAGA TTCACTTCCA GGGCTGGCGT 
CCATCCTACG ATCGCTGTGT GCGCGCCACC GTTCTATTGA AAGACACCGA GGAGAATCGC 
CAGTTGCAAA GGGAGCTGGC CGAAGCTGCC AAGCTGCAAA TTCGTGGTGA CTACTCCTAC 
AAGGGCACAC CCGATAAGCC ATCGGCCAAG AAGAAACGCG GCGGAAAAGC GGCCCACGTT 
GAGGAGCCAA TAGTGGTTCC AATGGACACG GGACATCTTG AGGCGGAGCA CGAAATGGCG 
CCCACTCCAC GAGCAGCGGG CAACCGGACG CGGGACAACA GCGGTGGCAA GCGAAAGGAA 
AAACCCCCGT CCGGAGATGG CCGATTAAAG GGAAACCGGG GCAGACAAAC AGAGACCTTT 
TATAACAATG CCATAAATGA TGTATCGGTC TATAACCATG TGCCACAGGA GGATCGCATT 
ATGATGCGAG TCAGCGAGCG ATTGCGAGAG CTTATAGAGT ACGATCGGAA TATGATCAAG 
GTACTAGGCA AACAGCACGC ACTGCCCGCC CGCGTGCCCA TCGTGACCAT CATGGAAAAC 
TTCGTCAAGC AGCAGGCCGT GGAGCTAGCC ATTAGCATTA AACAGGACAG CTCGAGGGCC 
AGGAACACGC AGAGTCGCAA TGCCAGAATG GAGCGGGAAT ACGATCGCGT TATGTCCACT 
GTCTGCATGC TGAAGGAGGT GGTAGACGGA TTGCGTATCT ACTTTGAGTT CCACGTGGAT 
GACCACTTGC TGTATACGGA GGAGAAGGAG TATGTCCACA ACTACCTCAC AGACGACAAC 
ATGCGCAACT GCAGCCTAAT ATTGAACAAG TCGTACGAGT ATATCAATCC CAGCGGTGAC 
ACGGAACTGA TCGGTCTGGA CGGCACGCCA GTGGTAGAAG GGTCGGGCGA CACCAATGGA 
CAGATCGGAG TTATTAATAT CGGCGGTCCG GAGTACGAGA AGCAGTTGCA AAAATGCCTG 
CTCTACATTG TTACCGCCAG CGGGAAAAAC ACTGCGCAGG CCTATGAGAG GACTTCCCCA 
TATACGGCTG CCTACAAACT GCCCGTGGAG ATGCGTGGAT TTCTGAACGA AACATTCAAG 
TGGCGGCTTT TGTCAGCGGA ATCGCCGCCA GAAAAGTCCA TGGTCTTTGG CGCTCCCCAC 
CTGGTTAGGC TAATGATTAA AATGCCCATG TTCTTAAACG CATCGCCGAT CAGCAACAAG 
AAGCTAGAAG ATCTACTTCC CCACCTCGAC GCCTTCATCA ACTACTTGGA GAACCACAGG 
GAGTGGTTCG ACAGGGAAAA CTTTGTTAAC TCCACTGCCC TGCCCCAGGA AGATCTGCAG 
CGGGAGCTGT TGGACTCCCT GGATGGGATT GCTGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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msl-3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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