Galphai cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Galphai
Entrez Gene ID	38765
Full Name	G protein alpha i subunit
Synonyms	CG10060,DG[[alpha1]],DGalpha1,Dmel\CG10060,G-alpha-65A,G-ia65A,G-ialpha65A,G-ialpha65a,G-oalpha65A,GNAI1/2/3,G[[alphai]],G[[i]],G[[i]]alpha,Gai,Galpha i,Galpha65A,Galpha[[i65A]],Galpha[[i]],Galpha[[i]]65A,Galphai/o,Galphaiota65A,Gi,Gi-alpha,Gi/0,Gialpha,galphai,i
General protein information	Preferred Names G protein alpha i subunit Names CG10060 gene product from transcript CG10060-RA CG10060-PA G protein I a-subunit 65A G protein alpha o subunit 65A G protein alpha subunit G protein alpha-subunit G protein alphai subunit G protein alphai subunit 65A G protein alphao subunit 65A Galphai-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 65D5-65D5

mRNA and Protein(s)

mRNA	Protein	Name
NM_058154.5	NP_477502.1	G protein alpha i subunit

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GNAI1	XP_001159548.1
Danio rerio (zebrafish)	gnai1	NP_957265.1
Rattus norvegicus (Norway rat)	Gnai1	NP_037277.1
Mus musculus (house mouse)	Gnai1	NP_034435.1
Gallus gallus (chicken)	GNAI1	NP_990734.1
Drosophila melanogaster (fruit fly)	G-ialpha65A	NP_477502.1
Xenopus tropicalis (tropical clawed frog)	gnai1	NP_001090865.1
Homo sapiens (human)	GNAI1	NP_002060.4
Bos taurus (cattle)	GNAI1	NP_776749.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005904	XP_315935.4
Caenorhabditis elegans (roundworm)	gpa-16	NP_490790.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Pins can mediate asymmetric spindle geometry.[PINs]. Comparisons of the phenotypes of Galphai, Ric-8, Gbeta13Fsingle and Ric-8;Gbeta13F double loss-of-function mutants indicate that, in NBs, Ric-8 positively regulates Gai activity.
Title: Ric-8 controls Drosophila neural progenitor asymmetric division by regulating heterotrimeric G proteins.

Galphai is not necessary for the control of the anteroposterior orientation of the mitotic spindle during pl cell division downstream of Frizzled signalling, but they are required for maintainance of the spindle within the plane of the epithelium
Title: Drosophila Ric-8 regulates Galphai cortical localization to promote Galphai-dependent planar orientation of the mitotic spindle during asymmetric cell division.

The following Galphai gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Galphai cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17740	NM_058154.5 Latest version!	Drosophila melanogaster G protein alpha i subunit (Galphai), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Galphai ( NM_058154.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477502.1 Drosophila melanogaster G protein alpha i subunit (Galphai), mRNA.

Price & Availability↑

CloneID	OFa17740
Clone ID Related Accession (Same CDS sequence)	NM_058154.5
Accession Version	NM_058154.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	G protein alpha i subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_058154.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTTGTG CCGTGAGTAC AGCACGCGAT AAGGAGGCCA TCGAGCGGTC GAAGAACATC 
GATCGCGCCC TGCGGGCGGA GGGCGAGCGG GCCGCCTCGG AGGTGAAACT GCTGCTGCTG 
GGAGCCGGAG AGTCGGGCAA GTCAACGATT GTGAAACAGA TGAAGATCAT CCATGACACG 
GGCTACTCGC AGGAGGAATG CGAGGAGTAT CGTCGCGTCG TCTTCAGCAA CACGGTGCAG 
AGTCTAATGG TGATCATCCG CGCAATGGGA CGCCTGAAGA TCGAGTTTGC CGATCCGAGC 
CGAACGGACA TTGCCCGCCA GTTCTTCACA CACGCCTCTG CCGCCGACGA GGGCATCCTG 
CTGCCCGAGA TCGTGCTCCT GATGAAGAAA CTCTGGGCAG ACGGTGGCGT ACAGCAGAGC 
TTCGCCCGAT CGCGCGAATA TCAGTTGAAT GACTCGGCGG GATACTATCT AAATTCGCTG 
GATAGGATCG CGCAACCGAA CTACATACCC ACGCAGCAGG ATGTGCTACG CACGCGGGTG 
AAGACCACTG GCATCATCGA GACACACTTC TCCTGCAAGC AGCTGCACTT CAAACTTTTC 
GATGTGGGTG GCCAGCGATC GGAGCGCAAA AAGTGGATTC ATTGCTTCGA GGGCGTTACG 
GCCATAATCT TCTGCGTCGC GCTATCAGGT TACGATCTAG TATTGGCCGA GGACGAAGAG 
ATGAATCGTA TGATTGAGTC CCTGAAGCTG TTCGATTCCA TCTGCAACTC CAAGTGGTTT 
GTGGAAACAT CCATAATACT GTTCCTCAAC AAGAAGGATC TGTTTGAGGA GAAGATCAAG 
AGATCTCCGC TGACGATATG CTTCCCAGAG TACACAGGAA CCAACACCTT CGAGGAGGCG 
GCCAACTACA TTCGCATGAA GTTCGAAAAC CTGAACAAGC GAAAAGACCA AAAGGAGATA 
TACACGCATC TCACCTGCGC CACGGATACG AACAACGTGA AGTTCGTCTT CGATGCCGTC 
ACCGATGTGA TCATCAAGAA CAATCTGAAA CAAATTGGCT TATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477502.1
CDS	407..1474
Translation	MGCAVSTARDKEAIERSKNIDRALRAEGERAASEVKLLLLGAGESGKSTIVKQMKIIHDTGYSQEECEEYRRVVFSNTVQSLMVIIRAMGRLKIEFADPSRTDIARQFFTHASAADEGILLPEIVLLMKKLWADGGVQQSFARSREYQLNDSAGYYLNSLDRIAQPNYIPTQQDVLRTRVKTTGIIETHFSCKQLHFKLFDVGGQRSERKKWIHCFEGVTAIIFCVALSGYDLVLAEDEEMNRMIESLKLFDSICNSKWFVETSIILFLNKKDLFEEKIKRSPLTICFPEYTGTNTFEEAANYIRMKFENLNKRKDQKEIYTHLTCATDTNNVKFVFDAVTDVIIKNNLKQIGLF

Target ORF information:

RefSeq Version	NM_058154.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster G protein alpha i subunit (Galphai), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058154.5

ORF Insert Sequence:

ATGGGTTGTG CCGTGAGTAC AGCACGCGAT AAGGAGGCCA TCGAGCGGTC GAAGAACATC 
GATCGCGCCC TGCGGGCGGA GGGCGAGCGG GCCGCCTCGG AGGTGAAACT GCTGCTGCTG 
GGAGCCGGAG AGTCGGGCAA GTCAACGATT GTGAAACAGA TGAAGATCAT CCATGACACG 
GGCTACTCGC AGGAGGAATG CGAGGAGTAT CGTCGCGTCG TCTTCAGCAA CACGGTGCAG 
AGTCTAATGG TGATCATCCG CGCAATGGGA CGCCTGAAGA TCGAGTTTGC CGATCCGAGC 
CGAACGGACA TTGCCCGCCA GTTCTTCACA CACGCCTCTG CCGCCGACGA GGGCATCCTG 
CTGCCCGAGA TCGTGCTCCT GATGAAGAAA CTCTGGGCAG ACGGTGGCGT ACAGCAGAGC 
TTCGCCCGAT CGCGCGAATA TCAGTTGAAT GACTCGGCGG GATACTATCT AAATTCGCTG 
GATAGGATCG CGCAACCGAA CTACATACCC ACGCAGCAGG ATGTGCTACG CACGCGGGTG 
AAGACCACTG GCATCATCGA GACACACTTC TCCTGCAAGC AGCTGCACTT CAAACTTTTC 
GATGTGGGTG GCCAGCGATC GGAGCGCAAA AAGTGGATTC ATTGCTTCGA GGGCGTTACG 
GCCATAATCT TCTGCGTCGC GCTATCAGGT TACGATCTAG TATTGGCCGA GGACGAAGAG 
ATGAATCGTA TGATTGAGTC CCTGAAGCTG TTCGATTCCA TCTGCAACTC CAAGTGGTTT 
GTGGAAACAT CCATAATACT GTTCCTCAAC AAGAAGGATC TGTTTGAGGA GAAGATCAAG 
AGATCTCCGC TGACGATATG CTTCCCAGAG TACACAGGAA CCAACACCTT CGAGGAGGCG 
GCCAACTACA TTCGCATGAA GTTCGAAAAC CTGAACAAGC GAAAAGACCA AAAGGAGATA 
TACACGCATC TCACCTGCGC CACGGATACG AACAACGTGA AGTTCGTCTT CGATGCCGTC 
ACCGATGTGA TCATCAAGAA CAATCTGAAA CAAATTGGCT TATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Galphai cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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