CG10077 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG10077
Entrez Gene ID	38756
Full Name	CG10077 gene product from transcript CG10077-RA
Synonyms	BcDNA:HL01868,BcDNA:HL07910,DmRH5,Dmel\CG10077,cg10077,p90
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 65D3-65D4

mRNA and Protein(s)

mRNA	Protein	Name
NM_168182.3	NP_729194.2	uncharacterized protein, isoform C
NM_001274562.1	NP_001261491.1	uncharacterized protein, isoform D
NM_139805.2	NP_648062.2	uncharacterized protein, isoform A

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	DDX5	XP_005624321.1
Gallus gallus (chicken)	DDX5	NP_990158.1
Kluyveromyces lactis	KLLA0F23716g	XP_456137.1
Schizosaccharomyces pombe (fission yeast)	dbp2	NP_596523.1
Rattus norvegicus (Norway rat)	Ddx5	NP_001007614.1
Saccharomyces cerevisiae (baker's yeast)	DBP2	NP_014287.3
Eremothecium gossypii	AGOS_AFL221C	NP_985329.2
Arabidopsis thaliana (thale cress)	AT5G63120	NP_974985.1
Homo sapiens (human)	DDX5	NP_004387.1
Bos taurus (cattle)	DDX5	NP_001178324.1
Mus musculus (house mouse)	Ddx5	NP_031866.2
Drosophila melanogaster (fruit fly)	CG10077	NP_648062.2
Neurospora crassa	NCU07839	XP_962960.2
Arabidopsis thaliana (thale cress)	AT1G55150	NP_175911.1
Pan troglodytes (chimpanzee)	DDX5	NP_001138306.1
Magnaporthe oryzae (rice blast fungus)	MGG_16901	XP_003712846.1
Xenopus tropicalis (tropical clawed frog)	ddx5	NP_989229.1
	DDX5	XP_001109860.1
Danio rerio (zebrafish)	ddx5	NP_997777.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG10077 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG10077 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17732	NM_168182.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG10077), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa17731	NM_001274562.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant D (CG10077), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa17731	NM_139805.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG10077), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG10077 ( NM_168182.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729194.2 Drosophila melanogaster uncharacterized protein, transcript variant C (CG10077), mRNA.

Price & Availability↑

CloneID	OFa17732
Clone ID Related Accession (Same CDS sequence)	NM_168182.3
Accession Version	NM_168182.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168182.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACATGT ACAACGGACA GATGAACGCC TTCGGAGGCG GTGGCGGCGG AGGAGCTGGT 
GGCCTAGCCG GCGGTATGGT GCCCAATCGC ATGGGCGGTG GTGCTCCCGG TGGCGGACCC 
GGCGGAGCGG GCATGTTCCA GCGCAATCGC TCGGCCCCAT ACCCAGGTTT CAATGGTCAT 
GGACCCGCCA ACGGTGGACA GCGACGCATG AACGGTGGTG GTCCTAGCAT GGGACCCGGC 
GGACCACGCA ACCAGGATGG ATTCGGTGGT CAGAACGGGG GCCAGCGCTC TTCCAATCAT 
GGCGCTCACT TGCCCAAGAT CGTTTGGTCT GAGGTGAATC TTACGCCATT CCGCAAGAAC 
TTTTACAAGC CGTGCGATTC GGTGCTGGCC AGGACTGTCG GTGAAACAGA GACCTTCCTC 
ACCAGCAATG AGATCACCAT CAAGGGCGAT CAGGTGCCCA CACCCAGTAT TGAATTCGAG 
GAGGGCGGCT TTCCCGATTA CGTGATGAAC GAGATCCGTA AGCAGGGTTT CGCCAAGCCA 
ACGGCCATTC AAGCCCAGGG TTGGCCCATT GCTATGAGTG GACGGGATCT GGTGGGCGTG 
GCCCAGACCG GATCGGGCAA GACCTTGGCG TATGTTCTGC CGGCGGTGGT GCACATCAAT 
AACCAGCCGC GCCTGGAGCG CGGTGATGGA CCCATCGCCC TGGTCCTGGC TCCCACTAGA 
GAGTTGGCCC AGCAGATCCA GCAGGTAGCC ATTGAGTTTG GCAGCAACAC GCATGTGCGC 
AACACTTGCA TCTTTGGTGG AGCGCCCAAG GGACAGCAGG CTAGGGATTT GGAGCGCGGC 
GTGGAGATTG TAATTGCCAC ACCAGGTCGC CTAATTGATT TCCTCGAGCG CGGCACCACC 
TCGTTGAAGA GATGCACCTA TCTGGTGCTT GACGAGGCCG ATCGTATGCT CGACATGGGC 
TTCGAGCCCC AGATCAGAAA GATCATGCAG CAGATCCGAC CGGACCGTCA GGTACTCATG 
TGGTCAGCTA CCTGGCCCAA AGAGGTGCGC CAGCTGGCCG AGGAATTCCT TAATAACTAC 
ATACAGGTGA ACATTGGTTC GCTTTCGCTG AGCGCCAATC ACAACATCCT CCAGATCGTG 
GACGTGTGCG ATGAGAATGA GAAATTGATG AAGCTGATCA AGCTGCTAAC AGACATCTCG 
GCGGAGAACG AGACCAAGAC CATCATTTTC GTGGAAACCA AGAAGCGTGT GGACGAGATC 
ACGCGCAATA TCTCCCGCCA GGGCTGGCGC GCATGCGCCA TCCACGGCGA TAAGTCGCAG 
CAGGAGCGCG ACTTTGTGCT CTCGAGCTTC CGCAATGGCC GGCACTCGAT ACTGGTGGCC 
ACCGATGTGG CTGCCCGCGG ACTCGGTGAG TATACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729194.2
CDS	261..1679
Translation	MNMYNGQMNAFGGGGGGGAGGLAGGMVPNRMGGGAPGGGPGGAGMFQRNRSAPYPGFNGHGPANGGQRRMNGGGPSMGPGGPRNQDGFGGQNGGQRSSNHGAHLPKIVWSEVNLTPFRKNFYKPCDSVLARTVGETETFLTSNEITIKGDQVPTPSIEFEEGGFPDYVMNEIRKQGFAKPTAIQAQGWPIAMSGRDLVGVAQTGSGKTLAYVLPAVVHINNQPRLERGDGPIALVLAPTRELAQQIQQVAIEFGSNTHVRNTCIFGGAPKGQQARDLERGVEIVIATPGRLIDFLERGTTSLKRCTYLVLDEADRMLDMGFEPQIRKIMQQIRPDRQVLMWSATWPKEVRQLAEEFLNNYIQVNIGSLSLSANHNILQIVDVCDENEKLMKLIKLLTDISAENETKTIIFVETKKRVDEITRNISRQGWRACAIHGDKSQQERDFVLSSFRNGRHSILVATDVAARGLGEYT

Target ORF information:

RefSeq Version	NM_168182.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG10077), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168182.3

ORF Insert Sequence:

ATGAACATGT ACAACGGACA GATGAACGCC TTCGGAGGCG GTGGCGGCGG AGGAGCTGGT 
GGCCTAGCCG GCGGTATGGT GCCCAATCGC ATGGGCGGTG GTGCTCCCGG TGGCGGACCC 
GGCGGAGCGG GCATGTTCCA GCGCAATCGC TCGGCCCCAT ACCCAGGTTT CAATGGTCAT 
GGACCCGCCA ACGGTGGACA GCGACGCATG AACGGTGGTG GTCCTAGCAT GGGACCCGGC 
GGACCACGCA ACCAGGATGG ATTCGGTGGT CAGAACGGGG GCCAGCGCTC TTCCAATCAT 
GGCGCTCACT TGCCCAAGAT CGTTTGGTCT GAGGTGAATC TTACGCCATT CCGCAAGAAC 
TTTTACAAGC CGTGCGATTC GGTGCTGGCC AGGACTGTCG GTGAAACAGA GACCTTCCTC 
ACCAGCAATG AGATCACCAT CAAGGGCGAT CAGGTGCCCA CACCCAGTAT TGAATTCGAG 
GAGGGCGGCT TTCCCGATTA CGTGATGAAC GAGATCCGTA AGCAGGGTTT CGCCAAGCCA 
ACGGCCATTC AAGCCCAGGG TTGGCCCATT GCTATGAGTG GACGGGATCT GGTGGGCGTG 
GCCCAGACCG GATCGGGCAA GACCTTGGCG TATGTTCTGC CGGCGGTGGT GCACATCAAT 
AACCAGCCGC GCCTGGAGCG CGGTGATGGA CCCATCGCCC TGGTCCTGGC TCCCACTAGA 
GAGTTGGCCC AGCAGATCCA GCAGGTAGCC ATTGAGTTTG GCAGCAACAC GCATGTGCGC 
AACACTTGCA TCTTTGGTGG AGCGCCCAAG GGACAGCAGG CTAGGGATTT GGAGCGCGGC 
GTGGAGATTG TAATTGCCAC ACCAGGTCGC CTAATTGATT TCCTCGAGCG CGGCACCACC 
TCGTTGAAGA GATGCACCTA TCTGGTGCTT GACGAGGCCG ATCGTATGCT CGACATGGGC 
TTCGAGCCCC AGATCAGAAA GATCATGCAG CAGATCCGAC CGGACCGTCA GGTACTCATG 
TGGTCAGCTA CCTGGCCCAA AGAGGTGCGC CAGCTGGCCG AGGAATTCCT TAATAACTAC 
ATACAGGTGA ACATTGGTTC GCTTTCGCTG AGCGCCAATC ACAACATCCT CCAGATCGTG 
GACGTGTGCG ATGAGAATGA GAAATTGATG AAGCTGATCA AGCTGCTAAC AGACATCTCG 
GCGGAGAACG AGACCAAGAC CATCATTTTC GTGGAAACCA AGAAGCGTGT GGACGAGATC 
ACGCGCAATA TCTCCCGCCA GGGCTGGCGC GCATGCGCCA TCCACGGCGA TAAGTCGCAG 
CAGGAGCGCG ACTTTGTGCT CTCGAGCTTC CGCAATGGCC GGCACTCGAT ACTGGTGGCC 
ACCGATGTGG CTGCCCGCGG ACTCGGTGAG TATACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG10077 ( NM_001274562.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261491.1 Drosophila melanogaster uncharacterized protein, transcript variant D (CG10077), mRNA.

Price & Availability↑

CloneID	OFa17731
Clone ID Related Accession (Same CDS sequence)	NM_001274562.1 , NM_139805.2
Accession Version	NM_001274562.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACATGT ACAACGGACA GATGAACGCC TTCGGAGGCG GTGGCGGCGG AGGAGCTGGT 
GGCCTAGCCG GCGGTATGGT GCCCAATCGC ATGGGCGGTG GTGCTCCCGG TGGCGGACCC 
GGCGGAGCGG GCATGTTCCA GCGCAATCGC TCGGCCCCAT ACCCAGGTTT CAATGGTCAT 
GGACCCGCCA ACGGTGGACA GCGACGCATG AACGGTGGTG GTCCTAGCAT GGGACCCGGC 
GGACCACGCA ACCAGGATGG ATTCGGTGGT CAGAACGGGG GCCAGCGCTC TTCCAATCAT 
GGCGCTCACT TGCCCAAGAT CGTTTGGTCT GAGGTGAATC TTACGCCATT CCGCAAGAAC 
TTTTACAAGC CGTGCGATTC GGTGCTGGCC AGGACTGTCG GTGAAACAGA GACCTTCCTC 
ACCAGCAATG AGATCACCAT CAAGGGCGAT CAGGTGCCCA CACCCAGTAT TGAATTCGAG 
GAGGGCGGCT TTCCCGATTA CGTGATGAAC GAGATCCGTA AGCAGGGTTT CGCCAAGCCA 
ACGGCCATTC AAGCCCAGGG TTGGCCCATT GCTATGAGTG GACGGGATCT GGTGGGCGTG 
GCCCAGACCG GATCGGGCAA GACCTTGGCG TATGTTCTGC CGGCGGTGGT GCACATCAAT 
AACCAGCCGC GCCTGGAGCG CGGTGATGGA CCCATCGCCC TGGTCCTGGC TCCCACTAGA 
GAGTTGGCCC AGCAGATCCA GCAGGTAGCC ATTGAGTTTG GCAGCAACAC GCATGTGCGC 
AACACTTGCA TCTTTGGTGG AGCGCCCAAG GGACAGCAGG CTAGGGATTT GGAGCGCGGC 
GTGGAGATTG TAATTGCCAC ACCAGGTCGC CTAATTGATT TCCTCGAGCG CGGCACCACC 
TCGTTGAAGA GATGCACCTA TCTGGTGCTT GACGAGGCCG ATCGTATGCT CGACATGGGC 
TTCGAGCCCC AGATCAGAAA GATCATGCAG CAGATCCGAC CGGACCGTCA GGTACTCATG 
TGGTCAGCTA CCTGGCCCAA AGAGGTGCGC CAGCTGGCCG AGGAATTCCT TAATAACTAC 
ATACAGGTGA ACATTGGTTC GCTTTCGCTG AGCGCCAATC ACAACATCCT CCAGATCGTG 
GACGTGTGCG ATGAGAATGA GAAATTGATG AAGCTGATCA AGCTGCTAAC AGACATCTCG 
GCGGAGAACG AGACCAAGAC CATCATTTTC GTGGAAACCA AGAAGCGTGT GGACGAGATC 
ACGCGCAATA TCTCCCGCCA GGGCTGGCGC GCATGCGCCA TCCACGGCGA TAAGTCGCAG 
CAGGAGCGCG ACTTTGTGCT CTCGAGCTTC CGCAATGGCC GGCACTCGAT ACTGGTGGCC 
ACCGATGTGG CTGCCCGCGG ACTCGACGTT GACGATGTCA AGTTTGTGAT CAACTACGAT 
TATCCTTCGA ACTCGGAGGA CTACGTTCAT CGCATTGGAC GCACCGGACG ATCCAATAAC 
ACCGGCACCG CCTACACCCT GTTTACGCAT TCCAACGCCA ACAAGGCCAA CGACTTGATC 
CAGGTGCTGC GCGAGGCCAA CCAGACCATC AATCCCAAAC TGATGAACAT GGCCATGAAC 
GGTGGCTACA ACAAGCGCGG TGGCGGCATG GGTGGCGGCT ACCGTGGCGG CAATGGCTAT 
CAGGGCCGCA ATCCCCAGAT GGGCGGTGGA TACAATGGCG GCAACAACTA CCGCAACAAC 
AATGGTCCCG GCGCCACCAT GAATCGCAAC TCCTTTAACG GCGGCAGCGC TGGCGGACCA 
CCACGCTTCG ATCAGAAGCC GCGCACTTCT CCGCCGAATC AGGGCGGACA GGGCGGCTAC 
CGTGGCCAGG GAGGCGGTGG CTACCAGGGA CAACAGCAGC AGCAGCAGCA GCAGAACGGC 
GGAGTGCCCT TCTCTCGTTT CAATCCCAAT GCTGCTTGCT TCGAGCCCAA CAAGCCGCAG 
AATGGTCCGG GATCTGGACC GCCACAGGCG CAGCACCAGC AGCAGCAACA GGCTCAGGCT 
GCCCAGCAGC AACACCTACT TCCTGATGCA TCCGTGCAGG CGGCGATGAC GGCTGCAAAC 
TACGGAGTTG ATCAGAAGCG CTCCCGTTTC TCGCCCTACA ACATGAACTT TGCCAACATG 
CCACCACCAG CGATGAACCA GCAGCAGGCG GCGGCGGCCC AGCAGCAACA GCAACAACAG 
TTGCAAACTC CCCAGCAGCA ACAGCAGCAG CAGCAAATGC AACAGCCACA GGCCTATGGC 
CAGTACTCTT CAATGTCCTC CAGCATGGCA ACCGTGTCGC TCAACGGAGG AGCTGCAGCG 
GTGGCCAGCT CTTACCGCGC CCCGTACGCA GTGCCCACGC AGGCGGCACC CTATATGATG 
GCGAACGGCG GCGACATCTT TGCCTATCCG CCGCCTCCGC TGCCTGTGCA GAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261491.1
CDS	125..2581
Translation	MNMYNGQMNAFGGGGGGGAGGLAGGMVPNRMGGGAPGGGPGGAGMFQRNRSAPYPGFNGHGPANGGQRRMNGGGPSMGPGGPRNQDGFGGQNGGQRSSNHGAHLPKIVWSEVNLTPFRKNFYKPCDSVLARTVGETETFLTSNEITIKGDQVPTPSIEFEEGGFPDYVMNEIRKQGFAKPTAIQAQGWPIAMSGRDLVGVAQTGSGKTLAYVLPAVVHINNQPRLERGDGPIALVLAPTRELAQQIQQVAIEFGSNTHVRNTCIFGGAPKGQQARDLERGVEIVIATPGRLIDFLERGTTSLKRCTYLVLDEADRMLDMGFEPQIRKIMQQIRPDRQVLMWSATWPKEVRQLAEEFLNNYIQVNIGSLSLSANHNILQIVDVCDENEKLMKLIKLLTDISAENETKTIIFVETKKRVDEITRNISRQGWRACAIHGDKSQQERDFVLSSFRNGRHSILVATDVAARGLDVDDVKFVINYDYPSNSEDYVHRIGRTGRSNNTGTAYTLFTHSNANKANDLIQVLREANQTINPKLMNMAMNGGYNKRGGGMGGGYRGGNGYQGRNPQMGGGYNGGNNYRNNNGPGATMNRNSFNGGSAGGPPRFDQKPRTSPPNQGGQGGYRGQGGGGYQGQQQQQQQQNGGVPFSRFNPNAACFEPNKPQNGPGSGPPQAQHQQQQQAQAAQQQHLLPDASVQAAMTAANYGVDQKRSRFSPYNMNFANMPPPAMNQQQAAAAQQQQQQQLQTPQQQQQQQQMQQPQAYGQYSSMSSSMATVSLNGGAAAVASSYRAPYAVPTQAAPYMMANGGDIFAYPPPPLPVQN

Target ORF information:

RefSeq Version	NM_001274562.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant D (CG10077), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274562.1

ORF Insert Sequence:

ATGAACATGT ACAACGGACA GATGAACGCC TTCGGAGGCG GTGGCGGCGG AGGAGCTGGT 
GGCCTAGCCG GCGGTATGGT GCCCAATCGC ATGGGCGGTG GTGCTCCCGG TGGCGGACCC 
GGCGGAGCGG GCATGTTCCA GCGCAATCGC TCGGCCCCAT ACCCAGGTTT CAATGGTCAT 
GGACCCGCCA ACGGTGGACA GCGACGCATG AACGGTGGTG GTCCTAGCAT GGGACCCGGC 
GGACCACGCA ACCAGGATGG ATTCGGTGGT CAGAACGGGG GCCAGCGCTC TTCCAATCAT 
GGCGCTCACT TGCCCAAGAT CGTTTGGTCT GAGGTGAATC TTACGCCATT CCGCAAGAAC 
TTTTACAAGC CGTGCGATTC GGTGCTGGCC AGGACTGTCG GTGAAACAGA GACCTTCCTC 
ACCAGCAATG AGATCACCAT CAAGGGCGAT CAGGTGCCCA CACCCAGTAT TGAATTCGAG 
GAGGGCGGCT TTCCCGATTA CGTGATGAAC GAGATCCGTA AGCAGGGTTT CGCCAAGCCA 
ACGGCCATTC AAGCCCAGGG TTGGCCCATT GCTATGAGTG GACGGGATCT GGTGGGCGTG 
GCCCAGACCG GATCGGGCAA GACCTTGGCG TATGTTCTGC CGGCGGTGGT GCACATCAAT 
AACCAGCCGC GCCTGGAGCG CGGTGATGGA CCCATCGCCC TGGTCCTGGC TCCCACTAGA 
GAGTTGGCCC AGCAGATCCA GCAGGTAGCC ATTGAGTTTG GCAGCAACAC GCATGTGCGC 
AACACTTGCA TCTTTGGTGG AGCGCCCAAG GGACAGCAGG CTAGGGATTT GGAGCGCGGC 
GTGGAGATTG TAATTGCCAC ACCAGGTCGC CTAATTGATT TCCTCGAGCG CGGCACCACC 
TCGTTGAAGA GATGCACCTA TCTGGTGCTT GACGAGGCCG ATCGTATGCT CGACATGGGC 
TTCGAGCCCC AGATCAGAAA GATCATGCAG CAGATCCGAC CGGACCGTCA GGTACTCATG 
TGGTCAGCTA CCTGGCCCAA AGAGGTGCGC CAGCTGGCCG AGGAATTCCT TAATAACTAC 
ATACAGGTGA ACATTGGTTC GCTTTCGCTG AGCGCCAATC ACAACATCCT CCAGATCGTG 
GACGTGTGCG ATGAGAATGA GAAATTGATG AAGCTGATCA AGCTGCTAAC AGACATCTCG 
GCGGAGAACG AGACCAAGAC CATCATTTTC GTGGAAACCA AGAAGCGTGT GGACGAGATC 
ACGCGCAATA TCTCCCGCCA GGGCTGGCGC GCATGCGCCA TCCACGGCGA TAAGTCGCAG 
CAGGAGCGCG ACTTTGTGCT CTCGAGCTTC CGCAATGGCC GGCACTCGAT ACTGGTGGCC 
ACCGATGTGG CTGCCCGCGG ACTCGACGTT GACGATGTCA AGTTTGTGAT CAACTACGAT 
TATCCTTCGA ACTCGGAGGA CTACGTTCAT CGCATTGGAC GCACCGGACG ATCCAATAAC 
ACCGGCACCG CCTACACCCT GTTTACGCAT TCCAACGCCA ACAAGGCCAA CGACTTGATC 
CAGGTGCTGC GCGAGGCCAA CCAGACCATC AATCCCAAAC TGATGAACAT GGCCATGAAC 
GGTGGCTACA ACAAGCGCGG TGGCGGCATG GGTGGCGGCT ACCGTGGCGG CAATGGCTAT 
CAGGGCCGCA ATCCCCAGAT GGGCGGTGGA TACAATGGCG GCAACAACTA CCGCAACAAC 
AATGGTCCCG GCGCCACCAT GAATCGCAAC TCCTTTAACG GCGGCAGCGC TGGCGGACCA 
CCACGCTTCG ATCAGAAGCC GCGCACTTCT CCGCCGAATC AGGGCGGACA GGGCGGCTAC 
CGTGGCCAGG GAGGCGGTGG CTACCAGGGA CAACAGCAGC AGCAGCAGCA GCAGAACGGC 
GGAGTGCCCT TCTCTCGTTT CAATCCCAAT GCTGCTTGCT TCGAGCCCAA CAAGCCGCAG 
AATGGTCCGG GATCTGGACC GCCACAGGCG CAGCACCAGC AGCAGCAACA GGCTCAGGCT 
GCCCAGCAGC AACACCTACT TCCTGATGCA TCCGTGCAGG CGGCGATGAC GGCTGCAAAC 
TACGGAGTTG ATCAGAAGCG CTCCCGTTTC TCGCCCTACA ACATGAACTT TGCCAACATG 
CCACCACCAG CGATGAACCA GCAGCAGGCG GCGGCGGCCC AGCAGCAACA GCAACAACAG 
TTGCAAACTC CCCAGCAGCA ACAGCAGCAG CAGCAAATGC AACAGCCACA GGCCTATGGC 
CAGTACTCTT CAATGTCCTC CAGCATGGCA ACCGTGTCGC TCAACGGAGG AGCTGCAGCG 
GTGGCCAGCT CTTACCGCGC CCCGTACGCA GTGCCCACGC AGGCGGCACC CTATATGATG 
GCGAACGGCG GCGACATCTT TGCCTATCCG CCGCCTCCGC TGCCTGTGCA GAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG10077 ( NM_139805.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648062.2 Drosophila melanogaster uncharacterized protein, transcript variant A (CG10077), mRNA.

Price & Availability↑

CloneID	OFa17731
Clone ID Related Accession (Same CDS sequence)	NM_001274562.1 , NM_139805.2
Accession Version	NM_139805.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Dec 17, 2009 this sequence version replaced NM_139805.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACATGT ACAACGGACA GATGAACGCC TTCGGAGGCG GTGGCGGCGG AGGAGCTGGT 
GGCCTAGCCG GCGGTATGGT GCCCAATCGC ATGGGCGGTG GTGCTCCCGG TGGCGGACCC 
GGCGGAGCGG GCATGTTCCA GCGCAATCGC TCGGCCCCAT ACCCAGGTTT CAATGGTCAT 
GGACCCGCCA ACGGTGGACA GCGACGCATG AACGGTGGTG GTCCTAGCAT GGGACCCGGC 
GGACCACGCA ACCAGGATGG ATTCGGTGGT CAGAACGGGG GCCAGCGCTC TTCCAATCAT 
GGCGCTCACT TGCCCAAGAT CGTTTGGTCT GAGGTGAATC TTACGCCATT CCGCAAGAAC 
TTTTACAAGC CGTGCGATTC GGTGCTGGCC AGGACTGTCG GTGAAACAGA GACCTTCCTC 
ACCAGCAATG AGATCACCAT CAAGGGCGAT CAGGTGCCCA CACCCAGTAT TGAATTCGAG 
GAGGGCGGCT TTCCCGATTA CGTGATGAAC GAGATCCGTA AGCAGGGTTT CGCCAAGCCA 
ACGGCCATTC AAGCCCAGGG TTGGCCCATT GCTATGAGTG GACGGGATCT GGTGGGCGTG 
GCCCAGACCG GATCGGGCAA GACCTTGGCG TATGTTCTGC CGGCGGTGGT GCACATCAAT 
AACCAGCCGC GCCTGGAGCG CGGTGATGGA CCCATCGCCC TGGTCCTGGC TCCCACTAGA 
GAGTTGGCCC AGCAGATCCA GCAGGTAGCC ATTGAGTTTG GCAGCAACAC GCATGTGCGC 
AACACTTGCA TCTTTGGTGG AGCGCCCAAG GGACAGCAGG CTAGGGATTT GGAGCGCGGC 
GTGGAGATTG TAATTGCCAC ACCAGGTCGC CTAATTGATT TCCTCGAGCG CGGCACCACC 
TCGTTGAAGA GATGCACCTA TCTGGTGCTT GACGAGGCCG ATCGTATGCT CGACATGGGC 
TTCGAGCCCC AGATCAGAAA GATCATGCAG CAGATCCGAC CGGACCGTCA GGTACTCATG 
TGGTCAGCTA CCTGGCCCAA AGAGGTGCGC CAGCTGGCCG AGGAATTCCT TAATAACTAC 
ATACAGGTGA ACATTGGTTC GCTTTCGCTG AGCGCCAATC ACAACATCCT CCAGATCGTG 
GACGTGTGCG ATGAGAATGA GAAATTGATG AAGCTGATCA AGCTGCTAAC AGACATCTCG 
GCGGAGAACG AGACCAAGAC CATCATTTTC GTGGAAACCA AGAAGCGTGT GGACGAGATC 
ACGCGCAATA TCTCCCGCCA GGGCTGGCGC GCATGCGCCA TCCACGGCGA TAAGTCGCAG 
CAGGAGCGCG ACTTTGTGCT CTCGAGCTTC CGCAATGGCC GGCACTCGAT ACTGGTGGCC 
ACCGATGTGG CTGCCCGCGG ACTCGACGTT GACGATGTCA AGTTTGTGAT CAACTACGAT 
TATCCTTCGA ACTCGGAGGA CTACGTTCAT CGCATTGGAC GCACCGGACG ATCCAATAAC 
ACCGGCACCG CCTACACCCT GTTTACGCAT TCCAACGCCA ACAAGGCCAA CGACTTGATC 
CAGGTGCTGC GCGAGGCCAA CCAGACCATC AATCCCAAAC TGATGAACAT GGCCATGAAC 
GGTGGCTACA ACAAGCGCGG TGGCGGCATG GGTGGCGGCT ACCGTGGCGG CAATGGCTAT 
CAGGGCCGCA ATCCCCAGAT GGGCGGTGGA TACAATGGCG GCAACAACTA CCGCAACAAC 
AATGGTCCCG GCGCCACCAT GAATCGCAAC TCCTTTAACG GCGGCAGCGC TGGCGGACCA 
CCACGCTTCG ATCAGAAGCC GCGCACTTCT CCGCCGAATC AGGGCGGACA GGGCGGCTAC 
CGTGGCCAGG GAGGCGGTGG CTACCAGGGA CAACAGCAGC AGCAGCAGCA GCAGAACGGC 
GGAGTGCCCT TCTCTCGTTT CAATCCCAAT GCTGCTTGCT TCGAGCCCAA CAAGCCGCAG 
AATGGTCCGG GATCTGGACC GCCACAGGCG CAGCACCAGC AGCAGCAACA GGCTCAGGCT 
GCCCAGCAGC AACACCTACT TCCTGATGCA TCCGTGCAGG CGGCGATGAC GGCTGCAAAC 
TACGGAGTTG ATCAGAAGCG CTCCCGTTTC TCGCCCTACA ACATGAACTT TGCCAACATG 
CCACCACCAG CGATGAACCA GCAGCAGGCG GCGGCGGCCC AGCAGCAACA GCAACAACAG 
TTGCAAACTC CCCAGCAGCA ACAGCAGCAG CAGCAAATGC AACAGCCACA GGCCTATGGC 
CAGTACTCTT CAATGTCCTC CAGCATGGCA ACCGTGTCGC TCAACGGAGG AGCTGCAGCG 
GTGGCCAGCT CTTACCGCGC CCCGTACGCA GTGCCCACGC AGGCGGCACC CTATATGATG 
GCGAACGGCG GCGACATCTT TGCCTATCCG CCGCCTCCGC TGCCTGTGCA GAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648062.2
CDS	248..2704
Translation	MNMYNGQMNAFGGGGGGGAGGLAGGMVPNRMGGGAPGGGPGGAGMFQRNRSAPYPGFNGHGPANGGQRRMNGGGPSMGPGGPRNQDGFGGQNGGQRSSNHGAHLPKIVWSEVNLTPFRKNFYKPCDSVLARTVGETETFLTSNEITIKGDQVPTPSIEFEEGGFPDYVMNEIRKQGFAKPTAIQAQGWPIAMSGRDLVGVAQTGSGKTLAYVLPAVVHINNQPRLERGDGPIALVLAPTRELAQQIQQVAIEFGSNTHVRNTCIFGGAPKGQQARDLERGVEIVIATPGRLIDFLERGTTSLKRCTYLVLDEADRMLDMGFEPQIRKIMQQIRPDRQVLMWSATWPKEVRQLAEEFLNNYIQVNIGSLSLSANHNILQIVDVCDENEKLMKLIKLLTDISAENETKTIIFVETKKRVDEITRNISRQGWRACAIHGDKSQQERDFVLSSFRNGRHSILVATDVAARGLDVDDVKFVINYDYPSNSEDYVHRIGRTGRSNNTGTAYTLFTHSNANKANDLIQVLREANQTINPKLMNMAMNGGYNKRGGGMGGGYRGGNGYQGRNPQMGGGYNGGNNYRNNNGPGATMNRNSFNGGSAGGPPRFDQKPRTSPPNQGGQGGYRGQGGGGYQGQQQQQQQQNGGVPFSRFNPNAACFEPNKPQNGPGSGPPQAQHQQQQQAQAAQQQHLLPDASVQAAMTAANYGVDQKRSRFSPYNMNFANMPPPAMNQQQAAAAQQQQQQQLQTPQQQQQQQQMQQPQAYGQYSSMSSSMATVSLNGGAAAVASSYRAPYAVPTQAAPYMMANGGDIFAYPPPPLPVQN

Target ORF information:

RefSeq Version	NM_139805.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG10077), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139805.2

ORF Insert Sequence:

ATGAACATGT ACAACGGACA GATGAACGCC TTCGGAGGCG GTGGCGGCGG AGGAGCTGGT 
GGCCTAGCCG GCGGTATGGT GCCCAATCGC ATGGGCGGTG GTGCTCCCGG TGGCGGACCC 
GGCGGAGCGG GCATGTTCCA GCGCAATCGC TCGGCCCCAT ACCCAGGTTT CAATGGTCAT 
GGACCCGCCA ACGGTGGACA GCGACGCATG AACGGTGGTG GTCCTAGCAT GGGACCCGGC 
GGACCACGCA ACCAGGATGG ATTCGGTGGT CAGAACGGGG GCCAGCGCTC TTCCAATCAT 
GGCGCTCACT TGCCCAAGAT CGTTTGGTCT GAGGTGAATC TTACGCCATT CCGCAAGAAC 
TTTTACAAGC CGTGCGATTC GGTGCTGGCC AGGACTGTCG GTGAAACAGA GACCTTCCTC 
ACCAGCAATG AGATCACCAT CAAGGGCGAT CAGGTGCCCA CACCCAGTAT TGAATTCGAG 
GAGGGCGGCT TTCCCGATTA CGTGATGAAC GAGATCCGTA AGCAGGGTTT CGCCAAGCCA 
ACGGCCATTC AAGCCCAGGG TTGGCCCATT GCTATGAGTG GACGGGATCT GGTGGGCGTG 
GCCCAGACCG GATCGGGCAA GACCTTGGCG TATGTTCTGC CGGCGGTGGT GCACATCAAT 
AACCAGCCGC GCCTGGAGCG CGGTGATGGA CCCATCGCCC TGGTCCTGGC TCCCACTAGA 
GAGTTGGCCC AGCAGATCCA GCAGGTAGCC ATTGAGTTTG GCAGCAACAC GCATGTGCGC 
AACACTTGCA TCTTTGGTGG AGCGCCCAAG GGACAGCAGG CTAGGGATTT GGAGCGCGGC 
GTGGAGATTG TAATTGCCAC ACCAGGTCGC CTAATTGATT TCCTCGAGCG CGGCACCACC 
TCGTTGAAGA GATGCACCTA TCTGGTGCTT GACGAGGCCG ATCGTATGCT CGACATGGGC 
TTCGAGCCCC AGATCAGAAA GATCATGCAG CAGATCCGAC CGGACCGTCA GGTACTCATG 
TGGTCAGCTA CCTGGCCCAA AGAGGTGCGC CAGCTGGCCG AGGAATTCCT TAATAACTAC 
ATACAGGTGA ACATTGGTTC GCTTTCGCTG AGCGCCAATC ACAACATCCT CCAGATCGTG 
GACGTGTGCG ATGAGAATGA GAAATTGATG AAGCTGATCA AGCTGCTAAC AGACATCTCG 
GCGGAGAACG AGACCAAGAC CATCATTTTC GTGGAAACCA AGAAGCGTGT GGACGAGATC 
ACGCGCAATA TCTCCCGCCA GGGCTGGCGC GCATGCGCCA TCCACGGCGA TAAGTCGCAG 
CAGGAGCGCG ACTTTGTGCT CTCGAGCTTC CGCAATGGCC GGCACTCGAT ACTGGTGGCC 
ACCGATGTGG CTGCCCGCGG ACTCGACGTT GACGATGTCA AGTTTGTGAT CAACTACGAT 
TATCCTTCGA ACTCGGAGGA CTACGTTCAT CGCATTGGAC GCACCGGACG ATCCAATAAC 
ACCGGCACCG CCTACACCCT GTTTACGCAT TCCAACGCCA ACAAGGCCAA CGACTTGATC 
CAGGTGCTGC GCGAGGCCAA CCAGACCATC AATCCCAAAC TGATGAACAT GGCCATGAAC 
GGTGGCTACA ACAAGCGCGG TGGCGGCATG GGTGGCGGCT ACCGTGGCGG CAATGGCTAT 
CAGGGCCGCA ATCCCCAGAT GGGCGGTGGA TACAATGGCG GCAACAACTA CCGCAACAAC 
AATGGTCCCG GCGCCACCAT GAATCGCAAC TCCTTTAACG GCGGCAGCGC TGGCGGACCA 
CCACGCTTCG ATCAGAAGCC GCGCACTTCT CCGCCGAATC AGGGCGGACA GGGCGGCTAC 
CGTGGCCAGG GAGGCGGTGG CTACCAGGGA CAACAGCAGC AGCAGCAGCA GCAGAACGGC 
GGAGTGCCCT TCTCTCGTTT CAATCCCAAT GCTGCTTGCT TCGAGCCCAA CAAGCCGCAG 
AATGGTCCGG GATCTGGACC GCCACAGGCG CAGCACCAGC AGCAGCAACA GGCTCAGGCT 
GCCCAGCAGC AACACCTACT TCCTGATGCA TCCGTGCAGG CGGCGATGAC GGCTGCAAAC 
TACGGAGTTG ATCAGAAGCG CTCCCGTTTC TCGCCCTACA ACATGAACTT TGCCAACATG 
CCACCACCAG CGATGAACCA GCAGCAGGCG GCGGCGGCCC AGCAGCAACA GCAACAACAG 
TTGCAAACTC CCCAGCAGCA ACAGCAGCAG CAGCAAATGC AACAGCCACA GGCCTATGGC 
CAGTACTCTT CAATGTCCTC CAGCATGGCA ACCGTGTCGC TCAACGGAGG AGCTGCAGCG 
GTGGCCAGCT CTTACCGCGC CCCGTACGCA GTGCCCACGC AGGCGGCACC CTATATGATG 
GCGAACGGCG GCGACATCTT TGCCTATCCG CCGCCTCCGC TGCCTGTGCA GAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG10077 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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