ple cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ple
Entrez Gene ID	38746
Full Name	pale
Synonyms	CG10118,DH65B,DTH,Dmel\CG10118,Pale,Ple,TH,Th,VII,dTH1,dTH65B,pale/ple,th
General protein information	Preferred Names pale Names CG10118 gene product from transcript CG10118-RC CG10118-PA CG10118-PB CG10118-PC Tyrosine-3-hydroxylase XBB70 group VII drosophila tyrosine hydroxylase ple-PA ple-PB ple-PC tyrosine hydrodroxylase tyrosine hydroxylase tyrosine-hydroxylase tyrosone hydroxylase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 65C3-65C3\|3-18 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_057549.3	NP_476897.1	pale, isoform A
NM_001300026.1	NP_001286955.1	pale, isoform C
NM_057550.4	NP_476898.1	pale, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	TH	NP_954987.2
Mus musculus (house mouse)	Th	NP_033403.1
Drosophila melanogaster (fruit fly)	ple	NP_476897.1
Bos taurus (cattle)	TH	NP_776309.1
Rattus norvegicus (Norway rat)	Th	NP_036872.1
	TH	XP_002799487.1
Canis lupus familiaris (dog)	TH	NP_001002966.1
Pan troglodytes (chimpanzee)	TH	XP_003312902.2
Gallus gallus (chicken)	TH	NP_990136.1
Danio rerio (zebrafish)	th	NP_571224.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006023	XP_316061.3
Xenopus tropicalis (tropical clawed frog)	LOC100488900	XP_002938944.2
Caenorhabditis elegans (roundworm)	cat-2	NP_001254009.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

activity of tyrosine hydroxylase is also increased by this interaction, in excess of the stimulation resulting from phosphorylation alone
Title: Direct binding of GTP cyclohydrolase and tyrosine hydroxylase: regulatory interactions between key enzymes in dopamine biosynthesis.

maternal as well as embryonic effects on the secretion and/or functionality of Tyrosine hydroxylase (pale) may play roles in the early developmental program of the organism.
Title: Maternal and embryonic sources of tyrosine hydroxylase during Drosophila embryogenesis.

The biochemically conserved regulatory mechanisms of recombinant DTH parallel those from mammals.
Title: Biochemical conservation of recombinant Drosophila tyrosine hydroxylase with its mammalian cognates.

The following ple gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ple cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17715	NM_057549.3 Latest version!	Drosophila melanogaster pale (ple), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa17716	NM_001300026.1 Latest version!	Drosophila melanogaster pale (ple), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa17716	NM_057550.4 Latest version!	Drosophila melanogaster pale (ple), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ple ( NM_057549.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476897.1 Drosophila melanogaster pale (ple), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa17715
Clone ID Related Accession (Same CDS sequence)	NM_057549.3
Accession Version	NM_057549.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pale, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_057549.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGGCCG TTGCAGCAGC CCAAAAGAAC CGCGAGATGT TCGCCATCAA GAAATCCTAC 
AGTATTGAGA ATGGCTATCC ATCCCGCCGT CGCAGCCTGG TGGATGATGC CCGTTTCGAG 
ACCCTGGTGG TCAAGCAGAC CAAACAAACC GTCCTCGAGG AGGCCCGCAG CAAGGCAAAT 
GATTACGGTC TCACCGAGGA CGAGATTTTG TTGGCCAATG CCGCCTCCGA ATCCTCGGAT 
GCCGAGGCTG CCATGCAGAG TGCCGCTTTG GTGGTCCGCC TCAAGGAGGG CATCTCCTCC 
TTGGGTCGCA TCCTCAAGGC CATCGAAACC TTCCACGGCA CCGTCCAGCA TGTGGAGTCC 
CGTCAGTCGC GCGTGGAGGG CGTGGACCAC GATGTCCTCA TCAAGTTGGA CATGACCCGT 
GGCAATCTGC TGCAGCTGAT CCGCTCCCTC AGGCAGTCGG GCTCCTTCAG CAGCATGAAT 
CTGATGGCCG ACAATAACTT GAATGTCAAG GCTCCGTGGT TCCCCAAGCA CGCCTCCGAA 
TTGGATAACT GCAACCATCT GATGACCAAG TACGAGCCCG ATTTGGACAT GAACCACCCC 
GGATTCGCCG ACAAGGTATA CCGCCAGCGT CGCAAGGAAA TTGCCGAGAT CGCATTCGCC 
TACAAGTACG GAGACCCGAT CCCATTCATC GACTACTCCG ATGTGGAGGT CAAGACCTGG 
CGCTCGGTGT TCAAGACCGT TCAGGATCTG GCTCCCAAGC ACGCCTGTGC CGAGTACCGG 
GCCGCCTTCC AGAAGCTCCA GGATGAGCAG ATCTTCGTGG AGACCCGTCT GCCCCAGTTG 
CAGGAGATGT CCGACTTTCT GCGCAAGAAC ACCGGATTCT CTCTCCGTCC TGCCGCCGGT 
CTTTTGACTG CCCGGGACTT CCTTGCCTCC TTGGCCTTCC GCATCTTCCA GAGCACCCAG 
TATGTGCGCC ACGTTAACTC ACCATACCAC ACCCCCGAGC CCGACTCCAT TCACGAGCTG 
CTGGGTCACA TGCCCCTGCT GGCCGATCCC AGCTTCGCCC AGTTCTCGCA GGAGATTGGA 
CTGGCCTCGC TGGGTGCCTC CGACGAAGAA ATCGAGAAGC TGTCCACGGT ATACTGGTTC 
ACTGTTGAGT TCGGTCTCTG CAAGGAACAT GGTCAGATCA AGGCCTACGG TGCTGGACTC 
CTGAGCTCCT ACGGTGAGCT GCTCCATGCC ATCAGCGACA AGTGCGAGCA CCGCGCCTTC 
GAGCCCGCAT CCACCGCCGT GCAGCCCTAC CAGGATCAGG AGTACCAGCC CATCTACTAT 
GTGGCCGAGA GCTTCGAGGA TGCCAAGGAC AAGTTCCGTC GCTGGGTGAG CACCATGTCG 
CGTCCATTCG AGGTGCGTTT CAACCCGCAC ACCGAGCGCG TCGAGGTGCT GGACTCCGTC 
GACAAGCTGG AGACTCTGGT GCACCAGATG AACACGGAGA TTTTGCATCT GACCAACGCC 
ATCTCCAAGT TGCGACGCCC GTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476897.1
CDS	453..1979
Translation	MMAVAAAQKNREMFAIKKSYSIENGYPSRRRSLVDDARFETLVVKQTKQTVLEEARSKANDYGLTEDEILLANAASESSDAEAAMQSAALVVRLKEGISSLGRILKAIETFHGTVQHVESRQSRVEGVDHDVLIKLDMTRGNLLQLIRSLRQSGSFSSMNLMADNNLNVKAPWFPKHASELDNCNHLMTKYEPDLDMNHPGFADKVYRQRRKEIAEIAFAYKYGDPIPFIDYSDVEVKTWRSVFKTVQDLAPKHACAEYRAAFQKLQDEQIFVETRLPQLQEMSDFLRKNTGFSLRPAAGLLTARDFLASLAFRIFQSTQYVRHVNSPYHTPEPDSIHELLGHMPLLADPSFAQFSQEIGLASLGASDEEIEKLSTVYWFTVEFGLCKEHGQIKAYGAGLLSSYGELLHAISDKCEHRAFEPASTAVQPYQDQEYQPIYYVAESFEDAKDKFRRWVSTMSRPFEVRFNPHTERVEVLDSVDKLETLVHQMNTEILHLTNAISKLRRPF

Target ORF information:

RefSeq Version	NM_057549.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pale (ple), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057549.3

ORF Insert Sequence:

ATGATGGCCG TTGCAGCAGC CCAAAAGAAC CGCGAGATGT TCGCCATCAA GAAATCCTAC 
AGTATTGAGA ATGGCTATCC ATCCCGCCGT CGCAGCCTGG TGGATGATGC CCGTTTCGAG 
ACCCTGGTGG TCAAGCAGAC CAAACAAACC GTCCTCGAGG AGGCCCGCAG CAAGGCAAAT 
GATTACGGTC TCACCGAGGA CGAGATTTTG TTGGCCAATG CCGCCTCCGA ATCCTCGGAT 
GCCGAGGCTG CCATGCAGAG TGCCGCTTTG GTGGTCCGCC TCAAGGAGGG CATCTCCTCC 
TTGGGTCGCA TCCTCAAGGC CATCGAAACC TTCCACGGCA CCGTCCAGCA TGTGGAGTCC 
CGTCAGTCGC GCGTGGAGGG CGTGGACCAC GATGTCCTCA TCAAGTTGGA CATGACCCGT 
GGCAATCTGC TGCAGCTGAT CCGCTCCCTC AGGCAGTCGG GCTCCTTCAG CAGCATGAAT 
CTGATGGCCG ACAATAACTT GAATGTCAAG GCTCCGTGGT TCCCCAAGCA CGCCTCCGAA 
TTGGATAACT GCAACCATCT GATGACCAAG TACGAGCCCG ATTTGGACAT GAACCACCCC 
GGATTCGCCG ACAAGGTATA CCGCCAGCGT CGCAAGGAAA TTGCCGAGAT CGCATTCGCC 
TACAAGTACG GAGACCCGAT CCCATTCATC GACTACTCCG ATGTGGAGGT CAAGACCTGG 
CGCTCGGTGT TCAAGACCGT TCAGGATCTG GCTCCCAAGC ACGCCTGTGC CGAGTACCGG 
GCCGCCTTCC AGAAGCTCCA GGATGAGCAG ATCTTCGTGG AGACCCGTCT GCCCCAGTTG 
CAGGAGATGT CCGACTTTCT GCGCAAGAAC ACCGGATTCT CTCTCCGTCC TGCCGCCGGT 
CTTTTGACTG CCCGGGACTT CCTTGCCTCC TTGGCCTTCC GCATCTTCCA GAGCACCCAG 
TATGTGCGCC ACGTTAACTC ACCATACCAC ACCCCCGAGC CCGACTCCAT TCACGAGCTG 
CTGGGTCACA TGCCCCTGCT GGCCGATCCC AGCTTCGCCC AGTTCTCGCA GGAGATTGGA 
CTGGCCTCGC TGGGTGCCTC CGACGAAGAA ATCGAGAAGC TGTCCACGGT ATACTGGTTC 
ACTGTTGAGT TCGGTCTCTG CAAGGAACAT GGTCAGATCA AGGCCTACGG TGCTGGACTC 
CTGAGCTCCT ACGGTGAGCT GCTCCATGCC ATCAGCGACA AGTGCGAGCA CCGCGCCTTC 
GAGCCCGCAT CCACCGCCGT GCAGCCCTAC CAGGATCAGG AGTACCAGCC CATCTACTAT 
GTGGCCGAGA GCTTCGAGGA TGCCAAGGAC AAGTTCCGTC GCTGGGTGAG CACCATGTCG 
CGTCCATTCG AGGTGCGTTT CAACCCGCAC ACCGAGCGCG TCGAGGTGCT GGACTCCGTC 
GACAAGCTGG AGACTCTGGT GCACCAGATG AACACGGAGA TTTTGCATCT GACCAACGCC 
ATCTCCAAGT TGCGACGCCC GTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ple ( NM_001300026.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286955.1 Drosophila melanogaster pale (ple), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa17716
Clone ID Related Accession (Same CDS sequence)	NM_001300026.1 , NM_057550.4
Accession Version	NM_001300026.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pale, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGGCCG TTGCAGCAGC CCAAAAGAAC CGCGAGATGT TCGCCATCAA GAAATCCTAC 
AGTATTGAGA ATGGCTATCC ATCCCGCCGT CGCAGCCTGG TGGATGATGC CCGTTTCGAG 
ACCCTGGTGG TCAAGCAGAC CAAACAAACC GTCCTCGAGG AGGCCCGCAG CAAGGCAAAT 
GACGATTCCC TGGAAGATTG CATTGTGCAG GCTCAGGAGC ACATTCCCTC CGAGCAAGAT 
GTGGAGCTCC AGGACGAGCA TGCCAATCTG GAGAACCTGC CCTTGGAGGA ATATGTTCCA 
GTTGAGGAGG ATGTTGAGTT TGAGAGCGTT GAACAGGAGC AGTCGGAGTC GCAGTCGCAG 
GAGCCAGAAG GCAACCAACA GCCGACCAAG AACGATTACG GTCTCACCGA GGACGAGATT 
TTGTTGGCCA ATGCCGCCTC CGAATCCTCG GATGCCGAGG CTGCCATGCA GAGTGCCGCT 
TTGGTGGTCC GCCTCAAGGA GGGCATCTCC TCCTTGGGTC GCATCCTCAA GGCCATCGAA 
ACCTTCCACG GCACCGTCCA GCATGTGGAG TCCCGTCAGT CGCGCGTGGA GGGCGTGGAC 
CACGATGTCC TCATCAAGTT GGACATGACC CGTGGCAATC TGCTGCAGCT GATCCGCTCC 
CTCAGGCAGT CGGGCTCCTT CAGCAGCATG AATCTGATGG CCGACAATAA CTTGAATGTC 
AAGGCTCCGT GGTTCCCCAA GCACGCCTCC GAATTGGATA ACTGCAACCA TCTGATGACC 
AAGTACGAGC CCGATTTGGA CATGAACCAC CCCGGATTCG CCGACAAGGT ATACCGCCAG 
CGTCGCAAGG AAATTGCCGA GATCGCATTC GCCTACAAGT ACGGAGACCC GATCCCATTC 
ATCGACTACT CCGATGTGGA GGTCAAGACC TGGCGCTCGG TGTTCAAGAC CGTTCAGGAT 
CTGGCTCCCA AGCACGCCTG TGCCGAGTAC CGGGCCGCCT TCCAGAAGCT CCAGGATGAG 
CAGATCTTCG TGGAGACCCG TCTGCCCCAG TTGCAGGAGA TGTCCGACTT TCTGCGCAAG 
AACACCGGAT TCTCTCTCCG TCCTGCCGCC GGTCTTTTGA CTGCCCGGGA CTTCCTTGCC 
TCCTTGGCCT TCCGCATCTT CCAGAGCACC CAGTATGTGC GCCACGTTAA CTCACCATAC 
CACACCCCCG AGCCCGACTC CATTCACGAG CTGCTGGGTC ACATGCCCCT GCTGGCCGAT 
CCCAGCTTCG CCCAGTTCTC GCAGGAGATT GGACTGGCCT CGCTGGGTGC CTCCGACGAA 
GAAATCGAGA AGCTGTCCAC GGTATACTGG TTCACTGTTG AGTTCGGTCT CTGCAAGGAA 
CATGGTCAGA TCAAGGCCTA CGGTGCTGGA CTCCTGAGCT CCTACGGTGA GCTGCTCCAT 
GCCATCAGCG ACAAGTGCGA GCACCGCGCC TTCGAGCCCG CATCCACCGC CGTGCAGCCC 
TACCAGGATC AGGAGTACCA GCCCATCTAC TATGTGGCCG AGAGCTTCGA GGATGCCAAG 
GACAAGTTCC GTCGCTGGGT GAGCACCATG TCGCGTCCAT TCGAGGTGCG TTTCAACCCG 
CACACCGAGC GCGTCGAGGT GCTGGACTCC GTCGACAAGC TGGAGACTCT GGTGCACCAG 
ATGAACACGG AGATTTTGCA TCTGACCAAC GCCATCTCCA AGTTGCGACG CCCGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286955.1
CDS	453..2192
Translation	MMAVAAAQKNREMFAIKKSYSIENGYPSRRRSLVDDARFETLVVKQTKQTVLEEARSKANDDSLEDCIVQAQEHIPSEQDVELQDEHANLENLPLEEYVPVEEDVEFESVEQEQSESQSQEPEGNQQPTKNDYGLTEDEILLANAASESSDAEAAMQSAALVVRLKEGISSLGRILKAIETFHGTVQHVESRQSRVEGVDHDVLIKLDMTRGNLLQLIRSLRQSGSFSSMNLMADNNLNVKAPWFPKHASELDNCNHLMTKYEPDLDMNHPGFADKVYRQRRKEIAEIAFAYKYGDPIPFIDYSDVEVKTWRSVFKTVQDLAPKHACAEYRAAFQKLQDEQIFVETRLPQLQEMSDFLRKNTGFSLRPAAGLLTARDFLASLAFRIFQSTQYVRHVNSPYHTPEPDSIHELLGHMPLLADPSFAQFSQEIGLASLGASDEEIEKLSTVYWFTVEFGLCKEHGQIKAYGAGLLSSYGELLHAISDKCEHRAFEPASTAVQPYQDQEYQPIYYVAESFEDAKDKFRRWVSTMSRPFEVRFNPHTERVEVLDSVDKLETLVHQMNTEILHLTNAISKLRRPF

Target ORF information:

RefSeq Version	NM_001300026.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pale (ple), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300026.1

ORF Insert Sequence:

ATGATGGCCG TTGCAGCAGC CCAAAAGAAC CGCGAGATGT TCGCCATCAA GAAATCCTAC 
AGTATTGAGA ATGGCTATCC ATCCCGCCGT CGCAGCCTGG TGGATGATGC CCGTTTCGAG 
ACCCTGGTGG TCAAGCAGAC CAAACAAACC GTCCTCGAGG AGGCCCGCAG CAAGGCAAAT 
GACGATTCCC TGGAAGATTG CATTGTGCAG GCTCAGGAGC ACATTCCCTC CGAGCAAGAT 
GTGGAGCTCC AGGACGAGCA TGCCAATCTG GAGAACCTGC CCTTGGAGGA ATATGTTCCA 
GTTGAGGAGG ATGTTGAGTT TGAGAGCGTT GAACAGGAGC AGTCGGAGTC GCAGTCGCAG 
GAGCCAGAAG GCAACCAACA GCCGACCAAG AACGATTACG GTCTCACCGA GGACGAGATT 
TTGTTGGCCA ATGCCGCCTC CGAATCCTCG GATGCCGAGG CTGCCATGCA GAGTGCCGCT 
TTGGTGGTCC GCCTCAAGGA GGGCATCTCC TCCTTGGGTC GCATCCTCAA GGCCATCGAA 
ACCTTCCACG GCACCGTCCA GCATGTGGAG TCCCGTCAGT CGCGCGTGGA GGGCGTGGAC 
CACGATGTCC TCATCAAGTT GGACATGACC CGTGGCAATC TGCTGCAGCT GATCCGCTCC 
CTCAGGCAGT CGGGCTCCTT CAGCAGCATG AATCTGATGG CCGACAATAA CTTGAATGTC 
AAGGCTCCGT GGTTCCCCAA GCACGCCTCC GAATTGGATA ACTGCAACCA TCTGATGACC 
AAGTACGAGC CCGATTTGGA CATGAACCAC CCCGGATTCG CCGACAAGGT ATACCGCCAG 
CGTCGCAAGG AAATTGCCGA GATCGCATTC GCCTACAAGT ACGGAGACCC GATCCCATTC 
ATCGACTACT CCGATGTGGA GGTCAAGACC TGGCGCTCGG TGTTCAAGAC CGTTCAGGAT 
CTGGCTCCCA AGCACGCCTG TGCCGAGTAC CGGGCCGCCT TCCAGAAGCT CCAGGATGAG 
CAGATCTTCG TGGAGACCCG TCTGCCCCAG TTGCAGGAGA TGTCCGACTT TCTGCGCAAG 
AACACCGGAT TCTCTCTCCG TCCTGCCGCC GGTCTTTTGA CTGCCCGGGA CTTCCTTGCC 
TCCTTGGCCT TCCGCATCTT CCAGAGCACC CAGTATGTGC GCCACGTTAA CTCACCATAC 
CACACCCCCG AGCCCGACTC CATTCACGAG CTGCTGGGTC ACATGCCCCT GCTGGCCGAT 
CCCAGCTTCG CCCAGTTCTC GCAGGAGATT GGACTGGCCT CGCTGGGTGC CTCCGACGAA 
GAAATCGAGA AGCTGTCCAC GGTATACTGG TTCACTGTTG AGTTCGGTCT CTGCAAGGAA 
CATGGTCAGA TCAAGGCCTA CGGTGCTGGA CTCCTGAGCT CCTACGGTGA GCTGCTCCAT 
GCCATCAGCG ACAAGTGCGA GCACCGCGCC TTCGAGCCCG CATCCACCGC CGTGCAGCCC 
TACCAGGATC AGGAGTACCA GCCCATCTAC TATGTGGCCG AGAGCTTCGA GGATGCCAAG 
GACAAGTTCC GTCGCTGGGT GAGCACCATG TCGCGTCCAT TCGAGGTGCG TTTCAACCCG 
CACACCGAGC GCGTCGAGGT GCTGGACTCC GTCGACAAGC TGGAGACTCT GGTGCACCAG 
ATGAACACGG AGATTTTGCA TCTGACCAAC GCCATCTCCA AGTTGCGACG CCCGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ple ( NM_057550.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476898.1 Drosophila melanogaster pale (ple), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa17716
Clone ID Related Accession (Same CDS sequence)	NM_001300026.1 , NM_057550.4
Accession Version	NM_057550.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pale, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_057550.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGGCCG TTGCAGCAGC CCAAAAGAAC CGCGAGATGT TCGCCATCAA GAAATCCTAC 
AGTATTGAGA ATGGCTATCC ATCCCGCCGT CGCAGCCTGG TGGATGATGC CCGTTTCGAG 
ACCCTGGTGG TCAAGCAGAC CAAACAAACC GTCCTCGAGG AGGCCCGCAG CAAGGCAAAT 
GACGATTCCC TGGAAGATTG CATTGTGCAG GCTCAGGAGC ACATTCCCTC CGAGCAAGAT 
GTGGAGCTCC AGGACGAGCA TGCCAATCTG GAGAACCTGC CCTTGGAGGA ATATGTTCCA 
GTTGAGGAGG ATGTTGAGTT TGAGAGCGTT GAACAGGAGC AGTCGGAGTC GCAGTCGCAG 
GAGCCAGAAG GCAACCAACA GCCGACCAAG AACGATTACG GTCTCACCGA GGACGAGATT 
TTGTTGGCCA ATGCCGCCTC CGAATCCTCG GATGCCGAGG CTGCCATGCA GAGTGCCGCT 
TTGGTGGTCC GCCTCAAGGA GGGCATCTCC TCCTTGGGTC GCATCCTCAA GGCCATCGAA 
ACCTTCCACG GCACCGTCCA GCATGTGGAG TCCCGTCAGT CGCGCGTGGA GGGCGTGGAC 
CACGATGTCC TCATCAAGTT GGACATGACC CGTGGCAATC TGCTGCAGCT GATCCGCTCC 
CTCAGGCAGT CGGGCTCCTT CAGCAGCATG AATCTGATGG CCGACAATAA CTTGAATGTC 
AAGGCTCCGT GGTTCCCCAA GCACGCCTCC GAATTGGATA ACTGCAACCA TCTGATGACC 
AAGTACGAGC CCGATTTGGA CATGAACCAC CCCGGATTCG CCGACAAGGT ATACCGCCAG 
CGTCGCAAGG AAATTGCCGA GATCGCATTC GCCTACAAGT ACGGAGACCC GATCCCATTC 
ATCGACTACT CCGATGTGGA GGTCAAGACC TGGCGCTCGG TGTTCAAGAC CGTTCAGGAT 
CTGGCTCCCA AGCACGCCTG TGCCGAGTAC CGGGCCGCCT TCCAGAAGCT CCAGGATGAG 
CAGATCTTCG TGGAGACCCG TCTGCCCCAG TTGCAGGAGA TGTCCGACTT TCTGCGCAAG 
AACACCGGAT TCTCTCTCCG TCCTGCCGCC GGTCTTTTGA CTGCCCGGGA CTTCCTTGCC 
TCCTTGGCCT TCCGCATCTT CCAGAGCACC CAGTATGTGC GCCACGTTAA CTCACCATAC 
CACACCCCCG AGCCCGACTC CATTCACGAG CTGCTGGGTC ACATGCCCCT GCTGGCCGAT 
CCCAGCTTCG CCCAGTTCTC GCAGGAGATT GGACTGGCCT CGCTGGGTGC CTCCGACGAA 
GAAATCGAGA AGCTGTCCAC GGTATACTGG TTCACTGTTG AGTTCGGTCT CTGCAAGGAA 
CATGGTCAGA TCAAGGCCTA CGGTGCTGGA CTCCTGAGCT CCTACGGTGA GCTGCTCCAT 
GCCATCAGCG ACAAGTGCGA GCACCGCGCC TTCGAGCCCG CATCCACCGC CGTGCAGCCC 
TACCAGGATC AGGAGTACCA GCCCATCTAC TATGTGGCCG AGAGCTTCGA GGATGCCAAG 
GACAAGTTCC GTCGCTGGGT GAGCACCATG TCGCGTCCAT TCGAGGTGCG TTTCAACCCG 
CACACCGAGC GCGTCGAGGT GCTGGACTCC GTCGACAAGC TGGAGACTCT GGTGCACCAG 
ATGAACACGG AGATTTTGCA TCTGACCAAC GCCATCTCCA AGTTGCGACG CCCGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476898.1
CDS	453..2192
Translation	MMAVAAAQKNREMFAIKKSYSIENGYPSRRRSLVDDARFETLVVKQTKQTVLEEARSKANDDSLEDCIVQAQEHIPSEQDVELQDEHANLENLPLEEYVPVEEDVEFESVEQEQSESQSQEPEGNQQPTKNDYGLTEDEILLANAASESSDAEAAMQSAALVVRLKEGISSLGRILKAIETFHGTVQHVESRQSRVEGVDHDVLIKLDMTRGNLLQLIRSLRQSGSFSSMNLMADNNLNVKAPWFPKHASELDNCNHLMTKYEPDLDMNHPGFADKVYRQRRKEIAEIAFAYKYGDPIPFIDYSDVEVKTWRSVFKTVQDLAPKHACAEYRAAFQKLQDEQIFVETRLPQLQEMSDFLRKNTGFSLRPAAGLLTARDFLASLAFRIFQSTQYVRHVNSPYHTPEPDSIHELLGHMPLLADPSFAQFSQEIGLASLGASDEEIEKLSTVYWFTVEFGLCKEHGQIKAYGAGLLSSYGELLHAISDKCEHRAFEPASTAVQPYQDQEYQPIYYVAESFEDAKDKFRRWVSTMSRPFEVRFNPHTERVEVLDSVDKLETLVHQMNTEILHLTNAISKLRRPF

Target ORF information:

RefSeq Version	NM_057550.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pale (ple), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057550.4

ORF Insert Sequence:

ATGATGGCCG TTGCAGCAGC CCAAAAGAAC CGCGAGATGT TCGCCATCAA GAAATCCTAC 
AGTATTGAGA ATGGCTATCC ATCCCGCCGT CGCAGCCTGG TGGATGATGC CCGTTTCGAG 
ACCCTGGTGG TCAAGCAGAC CAAACAAACC GTCCTCGAGG AGGCCCGCAG CAAGGCAAAT 
GACGATTCCC TGGAAGATTG CATTGTGCAG GCTCAGGAGC ACATTCCCTC CGAGCAAGAT 
GTGGAGCTCC AGGACGAGCA TGCCAATCTG GAGAACCTGC CCTTGGAGGA ATATGTTCCA 
GTTGAGGAGG ATGTTGAGTT TGAGAGCGTT GAACAGGAGC AGTCGGAGTC GCAGTCGCAG 
GAGCCAGAAG GCAACCAACA GCCGACCAAG AACGATTACG GTCTCACCGA GGACGAGATT 
TTGTTGGCCA ATGCCGCCTC CGAATCCTCG GATGCCGAGG CTGCCATGCA GAGTGCCGCT 
TTGGTGGTCC GCCTCAAGGA GGGCATCTCC TCCTTGGGTC GCATCCTCAA GGCCATCGAA 
ACCTTCCACG GCACCGTCCA GCATGTGGAG TCCCGTCAGT CGCGCGTGGA GGGCGTGGAC 
CACGATGTCC TCATCAAGTT GGACATGACC CGTGGCAATC TGCTGCAGCT GATCCGCTCC 
CTCAGGCAGT CGGGCTCCTT CAGCAGCATG AATCTGATGG CCGACAATAA CTTGAATGTC 
AAGGCTCCGT GGTTCCCCAA GCACGCCTCC GAATTGGATA ACTGCAACCA TCTGATGACC 
AAGTACGAGC CCGATTTGGA CATGAACCAC CCCGGATTCG CCGACAAGGT ATACCGCCAG 
CGTCGCAAGG AAATTGCCGA GATCGCATTC GCCTACAAGT ACGGAGACCC GATCCCATTC 
ATCGACTACT CCGATGTGGA GGTCAAGACC TGGCGCTCGG TGTTCAAGAC CGTTCAGGAT 
CTGGCTCCCA AGCACGCCTG TGCCGAGTAC CGGGCCGCCT TCCAGAAGCT CCAGGATGAG 
CAGATCTTCG TGGAGACCCG TCTGCCCCAG TTGCAGGAGA TGTCCGACTT TCTGCGCAAG 
AACACCGGAT TCTCTCTCCG TCCTGCCGCC GGTCTTTTGA CTGCCCGGGA CTTCCTTGCC 
TCCTTGGCCT TCCGCATCTT CCAGAGCACC CAGTATGTGC GCCACGTTAA CTCACCATAC 
CACACCCCCG AGCCCGACTC CATTCACGAG CTGCTGGGTC ACATGCCCCT GCTGGCCGAT 
CCCAGCTTCG CCCAGTTCTC GCAGGAGATT GGACTGGCCT CGCTGGGTGC CTCCGACGAA 
GAAATCGAGA AGCTGTCCAC GGTATACTGG TTCACTGTTG AGTTCGGTCT CTGCAAGGAA 
CATGGTCAGA TCAAGGCCTA CGGTGCTGGA CTCCTGAGCT CCTACGGTGA GCTGCTCCAT 
GCCATCAGCG ACAAGTGCGA GCACCGCGCC TTCGAGCCCG CATCCACCGC CGTGCAGCCC 
TACCAGGATC AGGAGTACCA GCCCATCTAC TATGTGGCCG AGAGCTTCGA GGATGCCAAG 
GACAAGTTCC GTCGCTGGGT GAGCACCATG TCGCGTCCAT TCGAGGTGCG TTTCAACCCG 
CACACCGAGC GCGTCGAGGT GCTGGACTCC GTCGACAAGC TGGAGACTCT GGTGCACCAG 
ATGAACACGG AGATTTTGCA TCTGACCAAC GCCATCTCCA AGTTGCGACG CCCGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ple cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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