CG8519 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG8519
Entrez Gene ID	38745
Full Name	CG8519 gene product from transcript CG8519-RA
Synonyms	Dmel\CG8519
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 65C3-65C3

mRNA and Protein(s)

mRNA	Protein	Name
NM_139797.4	NP_648054.2	uncharacterized protein, isoform A
NM_001274550.1	NP_001261479.1	uncharacterized protein, isoform B

Pathways from BioSystems

Homologies

Bos taurus (cattle)	RASL11A	XP_005213886.1
Drosophila melanogaster (fruit fly)	CG8519	NP_648054.2
	RASL11A	XP_001095327.1
Mus musculus (house mouse)	Rasl11a	NP_081140.1
Pan troglodytes (chimpanzee)	RASL11A	XP_001135793.1
Canis lupus familiaris (dog)	RASL11A	XP_003639922.1
Rattus norvegicus (Norway rat)	Rasl11a	NP_001002829.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006025	XP_316064.3
Xenopus tropicalis (tropical clawed frog)	rasl11a	XP_002941581.1
Homo sapiens (human)	RASL11A	NP_996563.1
Gallus gallus (chicken)	RASL11A	XP_417126.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG8519 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG8519 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17714	NM_139797.4 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG8519), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa20604	NM_001274550.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG8519), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG8519 ( NM_139797.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648054.2 Drosophila melanogaster uncharacterized protein, transcript variant A (CG8519), mRNA.

Price & Availability↑

CloneID	OFa17714
Clone ID Related Accession (Same CDS sequence)	NM_139797.4
Accession Version	NM_139797.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_139797.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCACGC GTGGAATTCG GTGCAAGAAG TCCACATTGA CGGAGTTGAA AATCGCCGTG 
ATCGGAGCGC CCAGTGTTGG CAAAAGCGCC CTAATTGTGC GTTTCTTGAC AAAGCGTTAC 
ATCGGGGAGT ACGATCATCA GACTGAGAAT CGCTACAAGC ACGAGGCTAT GGTGGATGGC 
GAACCGGTGC TCTTTGAAAT ACTAGACACA TGTCCCAAGG CTGAAGACGA ATACCCCAAT 
GCAGCTGAGC TCGTTCAGTG GGCCGATGGC CTATTGCTGG TCTACTCGAT AACAGATCGC 
AAGAGTTTCA ACTATATACG CCGTGCCAAA TCCGATCTTC AGTCCGATAC ACCCGTCCAA 
CTTTGTGCCA ACAAAGTGGA TATGGTGCAC CTGCGCCAGG TGAGCCGCGA CGAGGGCGAA 
ATCCTGGCCA AGGACTTTGA GTGCAAGTTC AGCGAGGTCT CCGCCGCCGA CCACGTCGAC 
CAGGTGGCCG AGGTTTTCAA CGAGCTGTGC AAAGAGGTGC TGGCCTCCAA GCGCAAGTCC 
AAGCAGAGTC TGCTGGAAAG GATGCTCGGT GGAGCACGAC CCTACAGCCG AGGGAAGAGC 
GACAGCAATT TGCCAAAAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648054.2
CDS	624..1247
Translation	MTTRGIRCKKSTLTELKIAVIGAPSVGKSALIVRFLTKRYIGEYDHQTENRYKHEAMVDGEPVLFEILDTCPKAEDEYPNAAELVQWADGLLLVYSITDRKSFNYIRRAKSDLQSDTPVQLCANKVDMVHLRQVSRDEGEILAKDFECKFSEVSAADHVDQVAEVFNELCKEVLASKRKSKQSLLERMLGGARPYSRGKSDSNLPKD

Target ORF information:

RefSeq Version	NM_139797.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG8519), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139797.4

ORF Insert Sequence:

ATGACCACGC GTGGAATTCG GTGCAAGAAG TCCACATTGA CGGAGTTGAA AATCGCCGTG 
ATCGGAGCGC CCAGTGTTGG CAAAAGCGCC CTAATTGTGC GTTTCTTGAC AAAGCGTTAC 
ATCGGGGAGT ACGATCATCA GACTGAGAAT CGCTACAAGC ACGAGGCTAT GGTGGATGGC 
GAACCGGTGC TCTTTGAAAT ACTAGACACA TGTCCCAAGG CTGAAGACGA ATACCCCAAT 
GCAGCTGAGC TCGTTCAGTG GGCCGATGGC CTATTGCTGG TCTACTCGAT AACAGATCGC 
AAGAGTTTCA ACTATATACG CCGTGCCAAA TCCGATCTTC AGTCCGATAC ACCCGTCCAA 
CTTTGTGCCA ACAAAGTGGA TATGGTGCAC CTGCGCCAGG TGAGCCGCGA CGAGGGCGAA 
ATCCTGGCCA AGGACTTTGA GTGCAAGTTC AGCGAGGTCT CCGCCGCCGA CCACGTCGAC 
CAGGTGGCCG AGGTTTTCAA CGAGCTGTGC AAAGAGGTGC TGGCCTCCAA GCGCAAGTCC 
AAGCAGAGTC TGCTGGAAAG GATGCTCGGT GGAGCACGAC CCTACAGCCG AGGGAAGAGC 
GACAGCAATT TGCCAAAAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG8519 ( NM_001274550.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261479.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG8519), mRNA.

Price & Availability↑

CloneID	OFa20604
Clone ID Related Accession (Same CDS sequence)	NM_001274550.1
Accession Version	NM_001274550.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGGATG GCGAACCGGT GCTCTTTGAA ATACTAGACA CATGTCCCAA GGCTGAAGAC 
GAATACCCCA ATGCAGCTGA GCTCGTTCAG TGGGCCGATG GCCTATTGCT GGTCTACTCG 
ATAACAGATC GCAAGAGTTT CAACTATATA CGCCGTGCCA AATCCGATCT TCAGTCCGAT 
ACACCCGTCC AACTTTGTGC CAACAAAGTG GATATGGTGC ACCTGCGCCA GGTGAGCCGC 
GACGAGGGCG AAATCCTGGC CAAGGACTTT GAGTGCAAGT TCAGCGAGGT CTCCGCCGCC 
GACCACGTCG ACCAGGTGGC CGAGGTTTTC AACGAGCTGT GCAAAGAGGT GCTGGCCTCC 
AAGCGCAAGT CCAAGCAGAG TCTGCTGGAA AGGATGCTCG GTGGAGCACG ACCCTACAGC 
CGAGGGAAGA GCGACAGCAA TTTGCCAAAA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261479.1
CDS	198..653
Translation	MVDGEPVLFEILDTCPKAEDEYPNAAELVQWADGLLLVYSITDRKSFNYIRRAKSDLQSDTPVQLCANKVDMVHLRQVSRDEGEILAKDFECKFSEVSAADHVDQVAEVFNELCKEVLASKRKSKQSLLERMLGGARPYSRGKSDSNLPKD

Target ORF information:

RefSeq Version	NM_001274550.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG8519), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274550.1

ORF Insert Sequence:

ATGGTGGATG GCGAACCGGT GCTCTTTGAA ATACTAGACA CATGTCCCAA GGCTGAAGAC 
GAATACCCCA ATGCAGCTGA GCTCGTTCAG TGGGCCGATG GCCTATTGCT GGTCTACTCG 
ATAACAGATC GCAAGAGTTT CAACTATATA CGCCGTGCCA AATCCGATCT TCAGTCCGAT 
ACACCCGTCC AACTTTGTGC CAACAAAGTG GATATGGTGC ACCTGCGCCA GGTGAGCCGC 
GACGAGGGCG AAATCCTGGC CAAGGACTTT GAGTGCAAGT TCAGCGAGGT CTCCGCCGCC 
GACCACGTCG ACCAGGTGGC CGAGGTTTTC AACGAGCTGT GCAAAGAGGT GCTGGCCTCC 
AAGCGCAAGT CCAAGCAGAG TCTGCTGGAA AGGATGCTCG GTGGAGCACG ACCCTACAGC 
CGAGGGAAGA GCGACAGCAA TTTGCCAAAA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG8519 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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