sfl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	sfl
Entrez Gene ID	38736
Full Name	sulfateless
Synonyms	CG8339,CT24567,Dmel\CG8339,NDST,Sfl,dw,l(3)03844,lincRNA.S4253
General protein information	Preferred Names sulfateless Names CG8339 gene product from transcript CG8339-RA CG8339-PA CG8339-PB diet-wingless sfl-PA sfl-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 65B3-65B4

mRNA and Protein(s)

mRNA	Protein	Name
NM_079222.5	NP_523946.1	sulfateless, isoform A
NM_001169883.3	NP_001163354.1	sulfateless, isoform B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NDST2	XP_005619103.1
Caenorhabditis elegans (roundworm)	hst-1	NP_501491.4
Homo sapiens (human)	NDST2	NP_003626.1
Pan troglodytes (chimpanzee)	NDST2	XP_507851.4
Mus musculus (house mouse)	Ndst2	NP_034941.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006328	XP_316352.3
Bos taurus (cattle)	NDST2	NP_777202.2
Danio rerio (zebrafish)	ndst2a	XP_001924050.1
Drosophila melanogaster (fruit fly)	sfl	NP_523946.1
Xenopus tropicalis (tropical clawed frog)	ndst2	NP_001136290.1
Gallus gallus (chicken)	NDST2	XP_421613.2
	NDST2	XP_001104406.2
Rattus norvegicus (Norway rat)	Ndst2	NP_001099210.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following sfl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sfl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17706	NM_079222.5 Latest version!	Drosophila melanogaster sulfateless (sfl), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa17706	NM_001169883.3 Latest version!	Drosophila melanogaster sulfateless (sfl), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sfl ( NM_079222.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523946.1 Drosophila melanogaster sulfateless (sfl), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa17706
Clone ID Related Accession (Same CDS sequence)	NM_079222.5 , NM_001169883.3
Accession Version	NM_079222.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sulfateless, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_079222.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAATAT CGGGCGGCAA TCAACATAAT AACAATGCAA ATCGCAAATA TGAAAAACTC 
ATTAAGCAGC CGCAGATGCA ATTTGGTAGC TCCGTGACCG GCACCCAAAC GGATGTGGAT 
TCCTGCCGAG ATGCGGACGC GGATGCGAAT GCTGTCCGCC AGGACTTTAG CAATTTCAAT 
AAGCATTTCG GCAATGGTCA TGCCATCACG GATCGCACAA TGCTGCTGCG TTTGGAGGAC 
GATGTCACCA CCGCCGCCGG CATTGTCACG TACAAGGGTA AATCGAATGG CAATGGAAAT 
GGGAATGGGA ACGGAAGCAT CGGGAGTATC AGTCTGGATT TCAACGGTAG TCCTACCTCA 
TCAACGTCAA TTGGCATCGC CAGCGGTAGC AGCAGCAACA CCCACCTGGC CAGTGGTGGT 
GGCGTTGGTG GAATCGGTGG CTCCGAGCCA GCCGGTTGGA TGTGCCATTG TTGTAATTTA 
ATTGCACGTC GCTGCTTTGG CATCAACGTT CGACGATGTG TCCTGGCGCT CCTGGCCATT 
ACGATGGTTA GCATTTTCTA CTACACGCAT TATGTCGATA CGGGCGTGTT TAATGGACTT 
ATTCAACGCG ACACGCATCC CGCACCCATT ATCAACTGCC GCATGATCAA CTCTGGTGGC 
AAACACATCC GGAATGCCTC CCCAGCGCCC GACCATCGTT CCGAGGCTCG ATTACGGATC 
GATCCCAAGG TCCTCGTTTT TGTGGAGACC ACATATTCCG GTCTCGGCAG GGACATTGCC 
GAGCTGCTCG TCTACAATCG GATCAAATAT AAAATCGAAG TGGCGGGCAA GAGTTTACCA 
GTTCTTACCA ATCTCGACAA AGGTCGTTAT GGCGTGATTG TGTTTGAGAA CCTGGATAAA 
TACCTCAATA TGGACAAATG GAATAGAGAG CTGTTGGACA AATACTGCCG GGAGTACTCC 
GTGGGAATTG TGGGTTTCGT GAGTCCCAGC GAAGAGACTC TTGTTGGTGC CCAACTTCGG 
GACTTTCCGC TGTTTGTTAA CACGAATCTG AGGTTGAGAG ACGCGAGCTT GAATCCCTTG 
TCATCAGTGC TTCGATTAAC TCGAGCTGGT GAAACAGCTT GGGGAGCTCT GCCCGGAGAT 
GATTGGGCGG TTTTCCAGCA TAATCACAGT ACCTATGAGC CAGTGGAGTG GGCGCAGAGA 
AACACTCAAG AGTATCCAGC GGATAGTGTG GGCCAGGTGC AACTACCTCT TACTACTGTC 
CTTCAAGATC GCGGACAGTT GGATGGCATA CAAAGAGTGC TATTCGGCAG CAGTTTGCGT 
TTTTGGCTTC ATCGTCTGGT CTTCCTGGAT GCTCTGAGCT ATCTAAGTCA TGGTCAACTT 
AGTTTGAATC TGGAGCGAAT GATTCTAGTC GATATAGATG ATATATTCGT GGGCGAGAAG 
GGCACCAGAC TGCGACCAGA TGATGTGAGA GCCCTAATAG CCACCCAGAA GAATATAGCA 
GCAATGGTTC CAGGTTTCCG GTTCAATCTG GGATTCTCGG GAAAATACTA TCATCATGGC 
ACGAGGGAAG AGAATCTGGG TGATGATTTC CTACTGCAGA ACGTTCAGGA GTTCAACTGG 
TTTTCGCACA TGTGGAAGCA CCAGCAGCCT CATTTGTATG ATAACCTAAC CCTCCTGATG 
GCAGAAATGC ACTTAAACTA CGCCTTTGCT GTGGATCACA ACATACCCAC GGATTCGGGT 
TACTCCATAT CACCGCACCA TTCAGGCGTT TACCCCGCCC ATGAGTTACT ATATATGGCT 
TGGAAAAAGG TGTGGAACGT GAAGGTTACG TCCACGGAAG AGTATCCGCA TTTGAGGCCA 
GCTCGATTGA GAAGAGGATT CATTCATCGC AACATAATGG TGTTGCCGAG GCAAACTTGT 
GGTCTATTCA CCCACACCAT GTACATCGAT CGCTACCCGG GGGGTAGAGA TAAACTAGAT 
GAATCCATAC AGGGTGGAGA ACTCTTCCAG ACCATTGTCT ATAATCCCAT CAACATCTTT 
ATGACGCACA TGTCCAACTA TGGATCGGAT CGTCTGGCTC TGTACACATT CCAATCGGTG 
ATCAAGTTCC TACAGTGCTG GACCAATCTT AAGTTGGCCT CAGCTCCGCC AGTTCAGCTG 
GCCGAGATGT ATTTCCGGCT GCATCCCGAG GAGGTGGATC CCGTGTGGGG AAATCCCTGT 
GACGATGTGC GCCACAAGAA GATCTGGTCA AAGACCAAGA ATTGCGATTC GTTGCCCAAG 
TTCTTGGTAA TTGGACCGCA GAAAACGGGC ACAACTGCAT TGTACACATT CCTATCCATG 
CATGGCAGTA TTGCGAGTAA CATCGCCAGT CCCGAAACCT TTGAGGAGGT TCAGTTCTTC 
AACGGAAACA ACTACTATCG TGGTCTGGAC TGGTACATGG ACTTCTTTCC ATCGGAATCG 
CTGCCCAACA CGAGTTCCCC GATGCCCACC CAGTTGGGAT CGCCACGTTT CATGTTCGAG 
AAGAGTGCTA CCTATTTCGA TGGCGAGGCT GTGCCCAAAC GAAGTCATGC CCTGCTTCCA 
CATGCAAAAA TCGTCACGAT TCTGATATCA CCTGCGAAGA GGGCCTACTC CTGGTACCAG 
CATCAGCGTT CCCACGGCGA TGTTATAGCC AACAACTATA GTTTCTATCA GGTCATAACG 
GCAAGTGACT CGGCACCGAG GGCACTGAAG GATCTGCGGA ACCGGTGCCT AAATCCCGGC 
AAGTATGCCC AGCACTTGGA GCACTGGCTG GCCTACTATC CCGCCCAGCA GCTGCACATC 
ATCGATGGCG AACAGTTGCG CCTGAATCCC ATCGATGTGA TGAACGAGCT GCAGCGCTTC 
CTCAAAATCC AGCCGCTGCT CGATTATTCA AATCACCTGC GATACGATGT GAAGAAGGGA 
TTCTACTGCC AGGCCGTCAG CGAGAAGCGC AATAAATGCC TCGGAAAGTC CAAGGGACGC 
CAGTATCCGG CGATGGATGA GCGCAGTGCC AAGCTGTTGC AGCGATACTA TCTCAATCAC 
AATACGGCGC TGGTGAAGCT GCTCAAAAAG CTGGGCTCGC GGCCAATACC GCAATGGCTC 
AAAGACGACC TGTCCACGGG AACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523946.1
CDS	1311..4457
Translation	MTISGGNQHNNNANRKYEKLIKQPQMQFGSSVTGTQTDVDSCRDADADANAVRQDFSNFNKHFGNGHAITDRTMLLRLEDDVTTAAGIVTYKGKSNGNGNGNGNGSIGSISLDFNGSPTSSTSIGIASGSSSNTHLASGGGVGGIGGSEPAGWMCHCCNLIARRCFGINVRRCVLALLAITMVSIFYYTHYVDTGVFNGLIQRDTHPAPIINCRMINSGGKHIRNASPAPDHRSEARLRIDPKVLVFVETTYSGLGRDIAELLVYNRIKYKIEVAGKSLPVLTNLDKGRYGVIVFENLDKYLNMDKWNRELLDKYCREYSVGIVGFVSPSEETLVGAQLRDFPLFVNTNLRLRDASLNPLSSVLRLTRAGETAWGALPGDDWAVFQHNHSTYEPVEWAQRNTQEYPADSVGQVQLPLTTVLQDRGQLDGIQRVLFGSSLRFWLHRLVFLDALSYLSHGQLSLNLERMILVDIDDIFVGEKGTRLRPDDVRALIATQKNIAAMVPGFRFNLGFSGKYYHHGTREENLGDDFLLQNVQEFNWFSHMWKHQQPHLYDNLTLLMAEMHLNYAFAVDHNIPTDSGYSISPHHSGVYPAHELLYMAWKKVWNVKVTSTEEYPHLRPARLRRGFIHRNIMVLPRQTCGLFTHTMYIDRYPGGRDKLDESIQGGELFQTIVYNPINIFMTHMSNYGSDRLALYTFQSVIKFLQCWTNLKLASAPPVQLAEMYFRLHPEEVDPVWGNPCDDVRHKKIWSKTKNCDSLPKFLVIGPQKTGTTALYTFLSMHGSIASNIASPETFEEVQFFNGNNYYRGLDWYMDFFPSESLPNTSSPMPTQLGSPRFMFEKSATYFDGEAVPKRSHALLPHAKIVTILISPAKRAYSWYQHQRSHGDVIANNYSFYQVITASDSAPRALKDLRNRCLNPGKYAQHLEHWLAYYPAQQLHIIDGEQLRLNPIDVMNELQRFLKIQPLLDYSNHLRYDVKKGFYCQAVSEKRNKCLGKSKGRQYPAMDERSAKLLQRYYLNHNTALVKLLKKLGSRPIPQWLKDDLSTGT

Target ORF information:

RefSeq Version	NM_079222.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sulfateless (sfl), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079222.5

ORF Insert Sequence:

ATGACAATAT CGGGCGGCAA TCAACATAAT AACAATGCAA ATCGCAAATA TGAAAAACTC 
ATTAAGCAGC CGCAGATGCA ATTTGGTAGC TCCGTGACCG GCACCCAAAC GGATGTGGAT 
TCCTGCCGAG ATGCGGACGC GGATGCGAAT GCTGTCCGCC AGGACTTTAG CAATTTCAAT 
AAGCATTTCG GCAATGGTCA TGCCATCACG GATCGCACAA TGCTGCTGCG TTTGGAGGAC 
GATGTCACCA CCGCCGCCGG CATTGTCACG TACAAGGGTA AATCGAATGG CAATGGAAAT 
GGGAATGGGA ACGGAAGCAT CGGGAGTATC AGTCTGGATT TCAACGGTAG TCCTACCTCA 
TCAACGTCAA TTGGCATCGC CAGCGGTAGC AGCAGCAACA CCCACCTGGC CAGTGGTGGT 
GGCGTTGGTG GAATCGGTGG CTCCGAGCCA GCCGGTTGGA TGTGCCATTG TTGTAATTTA 
ATTGCACGTC GCTGCTTTGG CATCAACGTT CGACGATGTG TCCTGGCGCT CCTGGCCATT 
ACGATGGTTA GCATTTTCTA CTACACGCAT TATGTCGATA CGGGCGTGTT TAATGGACTT 
ATTCAACGCG ACACGCATCC CGCACCCATT ATCAACTGCC GCATGATCAA CTCTGGTGGC 
AAACACATCC GGAATGCCTC CCCAGCGCCC GACCATCGTT CCGAGGCTCG ATTACGGATC 
GATCCCAAGG TCCTCGTTTT TGTGGAGACC ACATATTCCG GTCTCGGCAG GGACATTGCC 
GAGCTGCTCG TCTACAATCG GATCAAATAT AAAATCGAAG TGGCGGGCAA GAGTTTACCA 
GTTCTTACCA ATCTCGACAA AGGTCGTTAT GGCGTGATTG TGTTTGAGAA CCTGGATAAA 
TACCTCAATA TGGACAAATG GAATAGAGAG CTGTTGGACA AATACTGCCG GGAGTACTCC 
GTGGGAATTG TGGGTTTCGT GAGTCCCAGC GAAGAGACTC TTGTTGGTGC CCAACTTCGG 
GACTTTCCGC TGTTTGTTAA CACGAATCTG AGGTTGAGAG ACGCGAGCTT GAATCCCTTG 
TCATCAGTGC TTCGATTAAC TCGAGCTGGT GAAACAGCTT GGGGAGCTCT GCCCGGAGAT 
GATTGGGCGG TTTTCCAGCA TAATCACAGT ACCTATGAGC CAGTGGAGTG GGCGCAGAGA 
AACACTCAAG AGTATCCAGC GGATAGTGTG GGCCAGGTGC AACTACCTCT TACTACTGTC 
CTTCAAGATC GCGGACAGTT GGATGGCATA CAAAGAGTGC TATTCGGCAG CAGTTTGCGT 
TTTTGGCTTC ATCGTCTGGT CTTCCTGGAT GCTCTGAGCT ATCTAAGTCA TGGTCAACTT 
AGTTTGAATC TGGAGCGAAT GATTCTAGTC GATATAGATG ATATATTCGT GGGCGAGAAG 
GGCACCAGAC TGCGACCAGA TGATGTGAGA GCCCTAATAG CCACCCAGAA GAATATAGCA 
GCAATGGTTC CAGGTTTCCG GTTCAATCTG GGATTCTCGG GAAAATACTA TCATCATGGC 
ACGAGGGAAG AGAATCTGGG TGATGATTTC CTACTGCAGA ACGTTCAGGA GTTCAACTGG 
TTTTCGCACA TGTGGAAGCA CCAGCAGCCT CATTTGTATG ATAACCTAAC CCTCCTGATG 
GCAGAAATGC ACTTAAACTA CGCCTTTGCT GTGGATCACA ACATACCCAC GGATTCGGGT 
TACTCCATAT CACCGCACCA TTCAGGCGTT TACCCCGCCC ATGAGTTACT ATATATGGCT 
TGGAAAAAGG TGTGGAACGT GAAGGTTACG TCCACGGAAG AGTATCCGCA TTTGAGGCCA 
GCTCGATTGA GAAGAGGATT CATTCATCGC AACATAATGG TGTTGCCGAG GCAAACTTGT 
GGTCTATTCA CCCACACCAT GTACATCGAT CGCTACCCGG GGGGTAGAGA TAAACTAGAT 
GAATCCATAC AGGGTGGAGA ACTCTTCCAG ACCATTGTCT ATAATCCCAT CAACATCTTT 
ATGACGCACA TGTCCAACTA TGGATCGGAT CGTCTGGCTC TGTACACATT CCAATCGGTG 
ATCAAGTTCC TACAGTGCTG GACCAATCTT AAGTTGGCCT CAGCTCCGCC AGTTCAGCTG 
GCCGAGATGT ATTTCCGGCT GCATCCCGAG GAGGTGGATC CCGTGTGGGG AAATCCCTGT 
GACGATGTGC GCCACAAGAA GATCTGGTCA AAGACCAAGA ATTGCGATTC GTTGCCCAAG 
TTCTTGGTAA TTGGACCGCA GAAAACGGGC ACAACTGCAT TGTACACATT CCTATCCATG 
CATGGCAGTA TTGCGAGTAA CATCGCCAGT CCCGAAACCT TTGAGGAGGT TCAGTTCTTC 
AACGGAAACA ACTACTATCG TGGTCTGGAC TGGTACATGG ACTTCTTTCC ATCGGAATCG 
CTGCCCAACA CGAGTTCCCC GATGCCCACC CAGTTGGGAT CGCCACGTTT CATGTTCGAG 
AAGAGTGCTA CCTATTTCGA TGGCGAGGCT GTGCCCAAAC GAAGTCATGC CCTGCTTCCA 
CATGCAAAAA TCGTCACGAT TCTGATATCA CCTGCGAAGA GGGCCTACTC CTGGTACCAG 
CATCAGCGTT CCCACGGCGA TGTTATAGCC AACAACTATA GTTTCTATCA GGTCATAACG 
GCAAGTGACT CGGCACCGAG GGCACTGAAG GATCTGCGGA ACCGGTGCCT AAATCCCGGC 
AAGTATGCCC AGCACTTGGA GCACTGGCTG GCCTACTATC CCGCCCAGCA GCTGCACATC 
ATCGATGGCG AACAGTTGCG CCTGAATCCC ATCGATGTGA TGAACGAGCT GCAGCGCTTC 
CTCAAAATCC AGCCGCTGCT CGATTATTCA AATCACCTGC GATACGATGT GAAGAAGGGA 
TTCTACTGCC AGGCCGTCAG CGAGAAGCGC AATAAATGCC TCGGAAAGTC CAAGGGACGC 
CAGTATCCGG CGATGGATGA GCGCAGTGCC AAGCTGTTGC AGCGATACTA TCTCAATCAC 
AATACGGCGC TGGTGAAGCT GCTCAAAAAG CTGGGCTCGC GGCCAATACC GCAATGGCTC 
AAAGACGACC TGTCCACGGG AACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sfl ( NM_001169883.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163354.1 Drosophila melanogaster sulfateless (sfl), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa17706
Clone ID Related Accession (Same CDS sequence)	NM_079222.5 , NM_001169883.3
Accession Version	NM_001169883.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sulfateless, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001169883.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAATAT CGGGCGGCAA TCAACATAAT AACAATGCAA ATCGCAAATA TGAAAAACTC 
ATTAAGCAGC CGCAGATGCA ATTTGGTAGC TCCGTGACCG GCACCCAAAC GGATGTGGAT 
TCCTGCCGAG ATGCGGACGC GGATGCGAAT GCTGTCCGCC AGGACTTTAG CAATTTCAAT 
AAGCATTTCG GCAATGGTCA TGCCATCACG GATCGCACAA TGCTGCTGCG TTTGGAGGAC 
GATGTCACCA CCGCCGCCGG CATTGTCACG TACAAGGGTA AATCGAATGG CAATGGAAAT 
GGGAATGGGA ACGGAAGCAT CGGGAGTATC AGTCTGGATT TCAACGGTAG TCCTACCTCA 
TCAACGTCAA TTGGCATCGC CAGCGGTAGC AGCAGCAACA CCCACCTGGC CAGTGGTGGT 
GGCGTTGGTG GAATCGGTGG CTCCGAGCCA GCCGGTTGGA TGTGCCATTG TTGTAATTTA 
ATTGCACGTC GCTGCTTTGG CATCAACGTT CGACGATGTG TCCTGGCGCT CCTGGCCATT 
ACGATGGTTA GCATTTTCTA CTACACGCAT TATGTCGATA CGGGCGTGTT TAATGGACTT 
ATTCAACGCG ACACGCATCC CGCACCCATT ATCAACTGCC GCATGATCAA CTCTGGTGGC 
AAACACATCC GGAATGCCTC CCCAGCGCCC GACCATCGTT CCGAGGCTCG ATTACGGATC 
GATCCCAAGG TCCTCGTTTT TGTGGAGACC ACATATTCCG GTCTCGGCAG GGACATTGCC 
GAGCTGCTCG TCTACAATCG GATCAAATAT AAAATCGAAG TGGCGGGCAA GAGTTTACCA 
GTTCTTACCA ATCTCGACAA AGGTCGTTAT GGCGTGATTG TGTTTGAGAA CCTGGATAAA 
TACCTCAATA TGGACAAATG GAATAGAGAG CTGTTGGACA AATACTGCCG GGAGTACTCC 
GTGGGAATTG TGGGTTTCGT GAGTCCCAGC GAAGAGACTC TTGTTGGTGC CCAACTTCGG 
GACTTTCCGC TGTTTGTTAA CACGAATCTG AGGTTGAGAG ACGCGAGCTT GAATCCCTTG 
TCATCAGTGC TTCGATTAAC TCGAGCTGGT GAAACAGCTT GGGGAGCTCT GCCCGGAGAT 
GATTGGGCGG TTTTCCAGCA TAATCACAGT ACCTATGAGC CAGTGGAGTG GGCGCAGAGA 
AACACTCAAG AGTATCCAGC GGATAGTGTG GGCCAGGTGC AACTACCTCT TACTACTGTC 
CTTCAAGATC GCGGACAGTT GGATGGCATA CAAAGAGTGC TATTCGGCAG CAGTTTGCGT 
TTTTGGCTTC ATCGTCTGGT CTTCCTGGAT GCTCTGAGCT ATCTAAGTCA TGGTCAACTT 
AGTTTGAATC TGGAGCGAAT GATTCTAGTC GATATAGATG ATATATTCGT GGGCGAGAAG 
GGCACCAGAC TGCGACCAGA TGATGTGAGA GCCCTAATAG CCACCCAGAA GAATATAGCA 
GCAATGGTTC CAGGTTTCCG GTTCAATCTG GGATTCTCGG GAAAATACTA TCATCATGGC 
ACGAGGGAAG AGAATCTGGG TGATGATTTC CTACTGCAGA ACGTTCAGGA GTTCAACTGG 
TTTTCGCACA TGTGGAAGCA CCAGCAGCCT CATTTGTATG ATAACCTAAC CCTCCTGATG 
GCAGAAATGC ACTTAAACTA CGCCTTTGCT GTGGATCACA ACATACCCAC GGATTCGGGT 
TACTCCATAT CACCGCACCA TTCAGGCGTT TACCCCGCCC ATGAGTTACT ATATATGGCT 
TGGAAAAAGG TGTGGAACGT GAAGGTTACG TCCACGGAAG AGTATCCGCA TTTGAGGCCA 
GCTCGATTGA GAAGAGGATT CATTCATCGC AACATAATGG TGTTGCCGAG GCAAACTTGT 
GGTCTATTCA CCCACACCAT GTACATCGAT CGCTACCCGG GGGGTAGAGA TAAACTAGAT 
GAATCCATAC AGGGTGGAGA ACTCTTCCAG ACCATTGTCT ATAATCCCAT CAACATCTTT 
ATGACGCACA TGTCCAACTA TGGATCGGAT CGTCTGGCTC TGTACACATT CCAATCGGTG 
ATCAAGTTCC TACAGTGCTG GACCAATCTT AAGTTGGCCT CAGCTCCGCC AGTTCAGCTG 
GCCGAGATGT ATTTCCGGCT GCATCCCGAG GAGGTGGATC CCGTGTGGGG AAATCCCTGT 
GACGATGTGC GCCACAAGAA GATCTGGTCA AAGACCAAGA ATTGCGATTC GTTGCCCAAG 
TTCTTGGTAA TTGGACCGCA GAAAACGGGC ACAACTGCAT TGTACACATT CCTATCCATG 
CATGGCAGTA TTGCGAGTAA CATCGCCAGT CCCGAAACCT TTGAGGAGGT TCAGTTCTTC 
AACGGAAACA ACTACTATCG TGGTCTGGAC TGGTACATGG ACTTCTTTCC ATCGGAATCG 
CTGCCCAACA CGAGTTCCCC GATGCCCACC CAGTTGGGAT CGCCACGTTT CATGTTCGAG 
AAGAGTGCTA CCTATTTCGA TGGCGAGGCT GTGCCCAAAC GAAGTCATGC CCTGCTTCCA 
CATGCAAAAA TCGTCACGAT TCTGATATCA CCTGCGAAGA GGGCCTACTC CTGGTACCAG 
CATCAGCGTT CCCACGGCGA TGTTATAGCC AACAACTATA GTTTCTATCA GGTCATAACG 
GCAAGTGACT CGGCACCGAG GGCACTGAAG GATCTGCGGA ACCGGTGCCT AAATCCCGGC 
AAGTATGCCC AGCACTTGGA GCACTGGCTG GCCTACTATC CCGCCCAGCA GCTGCACATC 
ATCGATGGCG AACAGTTGCG CCTGAATCCC ATCGATGTGA TGAACGAGCT GCAGCGCTTC 
CTCAAAATCC AGCCGCTGCT CGATTATTCA AATCACCTGC GATACGATGT GAAGAAGGGA 
TTCTACTGCC AGGCCGTCAG CGAGAAGCGC AATAAATGCC TCGGAAAGTC CAAGGGACGC 
CAGTATCCGG CGATGGATGA GCGCAGTGCC AAGCTGTTGC AGCGATACTA TCTCAATCAC 
AATACGGCGC TGGTGAAGCT GCTCAAAAAG CTGGGCTCGC GGCCAATACC GCAATGGCTC 
AAAGACGACC TGTCCACGGG AACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163354.1
CDS	1274..4420
Translation	MTISGGNQHNNNANRKYEKLIKQPQMQFGSSVTGTQTDVDSCRDADADANAVRQDFSNFNKHFGNGHAITDRTMLLRLEDDVTTAAGIVTYKGKSNGNGNGNGNGSIGSISLDFNGSPTSSTSIGIASGSSSNTHLASGGGVGGIGGSEPAGWMCHCCNLIARRCFGINVRRCVLALLAITMVSIFYYTHYVDTGVFNGLIQRDTHPAPIINCRMINSGGKHIRNASPAPDHRSEARLRIDPKVLVFVETTYSGLGRDIAELLVYNRIKYKIEVAGKSLPVLTNLDKGRYGVIVFENLDKYLNMDKWNRELLDKYCREYSVGIVGFVSPSEETLVGAQLRDFPLFVNTNLRLRDASLNPLSSVLRLTRAGETAWGALPGDDWAVFQHNHSTYEPVEWAQRNTQEYPADSVGQVQLPLTTVLQDRGQLDGIQRVLFGSSLRFWLHRLVFLDALSYLSHGQLSLNLERMILVDIDDIFVGEKGTRLRPDDVRALIATQKNIAAMVPGFRFNLGFSGKYYHHGTREENLGDDFLLQNVQEFNWFSHMWKHQQPHLYDNLTLLMAEMHLNYAFAVDHNIPTDSGYSISPHHSGVYPAHELLYMAWKKVWNVKVTSTEEYPHLRPARLRRGFIHRNIMVLPRQTCGLFTHTMYIDRYPGGRDKLDESIQGGELFQTIVYNPINIFMTHMSNYGSDRLALYTFQSVIKFLQCWTNLKLASAPPVQLAEMYFRLHPEEVDPVWGNPCDDVRHKKIWSKTKNCDSLPKFLVIGPQKTGTTALYTFLSMHGSIASNIASPETFEEVQFFNGNNYYRGLDWYMDFFPSESLPNTSSPMPTQLGSPRFMFEKSATYFDGEAVPKRSHALLPHAKIVTILISPAKRAYSWYQHQRSHGDVIANNYSFYQVITASDSAPRALKDLRNRCLNPGKYAQHLEHWLAYYPAQQLHIIDGEQLRLNPIDVMNELQRFLKIQPLLDYSNHLRYDVKKGFYCQAVSEKRNKCLGKSKGRQYPAMDERSAKLLQRYYLNHNTALVKLLKKLGSRPIPQWLKDDLSTGT

Target ORF information:

RefSeq Version	NM_001169883.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sulfateless (sfl), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169883.3

ORF Insert Sequence:

ATGACAATAT CGGGCGGCAA TCAACATAAT AACAATGCAA ATCGCAAATA TGAAAAACTC 
ATTAAGCAGC CGCAGATGCA ATTTGGTAGC TCCGTGACCG GCACCCAAAC GGATGTGGAT 
TCCTGCCGAG ATGCGGACGC GGATGCGAAT GCTGTCCGCC AGGACTTTAG CAATTTCAAT 
AAGCATTTCG GCAATGGTCA TGCCATCACG GATCGCACAA TGCTGCTGCG TTTGGAGGAC 
GATGTCACCA CCGCCGCCGG CATTGTCACG TACAAGGGTA AATCGAATGG CAATGGAAAT 
GGGAATGGGA ACGGAAGCAT CGGGAGTATC AGTCTGGATT TCAACGGTAG TCCTACCTCA 
TCAACGTCAA TTGGCATCGC CAGCGGTAGC AGCAGCAACA CCCACCTGGC CAGTGGTGGT 
GGCGTTGGTG GAATCGGTGG CTCCGAGCCA GCCGGTTGGA TGTGCCATTG TTGTAATTTA 
ATTGCACGTC GCTGCTTTGG CATCAACGTT CGACGATGTG TCCTGGCGCT CCTGGCCATT 
ACGATGGTTA GCATTTTCTA CTACACGCAT TATGTCGATA CGGGCGTGTT TAATGGACTT 
ATTCAACGCG ACACGCATCC CGCACCCATT ATCAACTGCC GCATGATCAA CTCTGGTGGC 
AAACACATCC GGAATGCCTC CCCAGCGCCC GACCATCGTT CCGAGGCTCG ATTACGGATC 
GATCCCAAGG TCCTCGTTTT TGTGGAGACC ACATATTCCG GTCTCGGCAG GGACATTGCC 
GAGCTGCTCG TCTACAATCG GATCAAATAT AAAATCGAAG TGGCGGGCAA GAGTTTACCA 
GTTCTTACCA ATCTCGACAA AGGTCGTTAT GGCGTGATTG TGTTTGAGAA CCTGGATAAA 
TACCTCAATA TGGACAAATG GAATAGAGAG CTGTTGGACA AATACTGCCG GGAGTACTCC 
GTGGGAATTG TGGGTTTCGT GAGTCCCAGC GAAGAGACTC TTGTTGGTGC CCAACTTCGG 
GACTTTCCGC TGTTTGTTAA CACGAATCTG AGGTTGAGAG ACGCGAGCTT GAATCCCTTG 
TCATCAGTGC TTCGATTAAC TCGAGCTGGT GAAACAGCTT GGGGAGCTCT GCCCGGAGAT 
GATTGGGCGG TTTTCCAGCA TAATCACAGT ACCTATGAGC CAGTGGAGTG GGCGCAGAGA 
AACACTCAAG AGTATCCAGC GGATAGTGTG GGCCAGGTGC AACTACCTCT TACTACTGTC 
CTTCAAGATC GCGGACAGTT GGATGGCATA CAAAGAGTGC TATTCGGCAG CAGTTTGCGT 
TTTTGGCTTC ATCGTCTGGT CTTCCTGGAT GCTCTGAGCT ATCTAAGTCA TGGTCAACTT 
AGTTTGAATC TGGAGCGAAT GATTCTAGTC GATATAGATG ATATATTCGT GGGCGAGAAG 
GGCACCAGAC TGCGACCAGA TGATGTGAGA GCCCTAATAG CCACCCAGAA GAATATAGCA 
GCAATGGTTC CAGGTTTCCG GTTCAATCTG GGATTCTCGG GAAAATACTA TCATCATGGC 
ACGAGGGAAG AGAATCTGGG TGATGATTTC CTACTGCAGA ACGTTCAGGA GTTCAACTGG 
TTTTCGCACA TGTGGAAGCA CCAGCAGCCT CATTTGTATG ATAACCTAAC CCTCCTGATG 
GCAGAAATGC ACTTAAACTA CGCCTTTGCT GTGGATCACA ACATACCCAC GGATTCGGGT 
TACTCCATAT CACCGCACCA TTCAGGCGTT TACCCCGCCC ATGAGTTACT ATATATGGCT 
TGGAAAAAGG TGTGGAACGT GAAGGTTACG TCCACGGAAG AGTATCCGCA TTTGAGGCCA 
GCTCGATTGA GAAGAGGATT CATTCATCGC AACATAATGG TGTTGCCGAG GCAAACTTGT 
GGTCTATTCA CCCACACCAT GTACATCGAT CGCTACCCGG GGGGTAGAGA TAAACTAGAT 
GAATCCATAC AGGGTGGAGA ACTCTTCCAG ACCATTGTCT ATAATCCCAT CAACATCTTT 
ATGACGCACA TGTCCAACTA TGGATCGGAT CGTCTGGCTC TGTACACATT CCAATCGGTG 
ATCAAGTTCC TACAGTGCTG GACCAATCTT AAGTTGGCCT CAGCTCCGCC AGTTCAGCTG 
GCCGAGATGT ATTTCCGGCT GCATCCCGAG GAGGTGGATC CCGTGTGGGG AAATCCCTGT 
GACGATGTGC GCCACAAGAA GATCTGGTCA AAGACCAAGA ATTGCGATTC GTTGCCCAAG 
TTCTTGGTAA TTGGACCGCA GAAAACGGGC ACAACTGCAT TGTACACATT CCTATCCATG 
CATGGCAGTA TTGCGAGTAA CATCGCCAGT CCCGAAACCT TTGAGGAGGT TCAGTTCTTC 
AACGGAAACA ACTACTATCG TGGTCTGGAC TGGTACATGG ACTTCTTTCC ATCGGAATCG 
CTGCCCAACA CGAGTTCCCC GATGCCCACC CAGTTGGGAT CGCCACGTTT CATGTTCGAG 
AAGAGTGCTA CCTATTTCGA TGGCGAGGCT GTGCCCAAAC GAAGTCATGC CCTGCTTCCA 
CATGCAAAAA TCGTCACGAT TCTGATATCA CCTGCGAAGA GGGCCTACTC CTGGTACCAG 
CATCAGCGTT CCCACGGCGA TGTTATAGCC AACAACTATA GTTTCTATCA GGTCATAACG 
GCAAGTGACT CGGCACCGAG GGCACTGAAG GATCTGCGGA ACCGGTGCCT AAATCCCGGC 
AAGTATGCCC AGCACTTGGA GCACTGGCTG GCCTACTATC CCGCCCAGCA GCTGCACATC 
ATCGATGGCG AACAGTTGCG CCTGAATCCC ATCGATGTGA TGAACGAGCT GCAGCGCTTC 
CTCAAAATCC AGCCGCTGCT CGATTATTCA AATCACCTGC GATACGATGT GAAGAAGGGA 
TTCTACTGCC AGGCCGTCAG CGAGAAGCGC AATAAATGCC TCGGAAAGTC CAAGGGACGC 
CAGTATCCGG CGATGGATGA GCGCAGTGCC AAGCTGTTGC AGCGATACTA TCTCAATCAC 
AATACGGCGC TGGTGAAGCT GCTCAAAAAG CTGGGCTCGC GGCCAATACC GCAATGGCTC 
AAAGACGACC TGTCCACGGG AACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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sfl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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