pav cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	pav
Entrez Gene ID	38515
Full Name	pavarotti
Synonyms	0934/13,0973/08,2A12,2A12MEL,CG1258,DmKLP1/pav,DmMKLP1,DmPAV,DmPav,Dmel\CG1258,KIF 23,KIF23,Mklp1,PAV,PAV-KLP,PAV-MKLP,PAV/KLP,Pav,Pav KLP,Pav-KLP,Pav-Klp,Pav-klp,PavKLP,anon-EST:fe2A12,anon2A12,group D,pav-KLP,pav-Klp
General protein information	Preferred Names pavarotti Names CG1258 gene product from transcript CG1258-RA CG1258-PA Kinesin-6 Kinesin6 Pavarotti-KLP anon-fast-evolving-2A12 mitotic kinesin-like protein 1 pav-PA pavarotti KLP pavarotti kinesin-like protein pavarroti
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 64A10-64A11

mRNA and Protein(s)

mRNA	Protein	Name
NM_057677.4	NP_477025.1	pavarotti

Pathways from BioSystems

Homologies

Homo sapiens (human)	KIF23	NP_612565.1
Pan troglodytes (chimpanzee)	KIF23	XP_001174899.2
Rattus norvegicus (Norway rat)	Kif23	NP_001101625.1
Drosophila melanogaster (fruit fly)	pav	NP_477025.1
Mus musculus (house mouse)	Kif23	NP_077207.3
Gallus gallus (chicken)	KIF23	NP_001025725.1
Canis lupus familiaris (dog)	KIF23	XP_005638602.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007706	XP_308170.4
Danio rerio (zebrafish)	kif23	NP_571430.1
Caenorhabditis elegans (roundworm)	zen-4	NP_001023309.1
Xenopus tropicalis (tropical clawed frog)	kif23	NP_001011104.1
	KIF23	XP_001086180.2
Bos taurus (cattle)	KIF23	NP_001091507.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

elevated expression of Pav-KLP underlies transport and plasticity phenotypes in pathway mutants, indicating that Toll-6-FoxO signaling promotes MT dynamics by limiting Pav-KLP expression.
Title: A Toll receptor-FoxO pathway represses Pavarotti/MKLP1 to promote microtubule dynamics in motoneurons.

Data show that GTPase-activating protein (Tum/RacGAP) is required for the motor activity of kinesin-6 motor (Pav/kinesin-6).
Title: Tum/RacGAP functions as a switch activating the Pav/kinesin-6 motor.

Depletion of Pav-KLP in neurons strongly stimulated kinesin-1-driven sliding of long microtubules and neurite outgrowth, while its ectopic overexpression in the cytoplasm blocked both of these processes.
Title: Pavarotti/MKLP1 regulates microtubule sliding and neurite outgrowth in Drosophila neurons.

Study demonstrates the emerging role of centralspindlin (complex of two proteins Tum and Pav) in regulating cell migration and cell adhesion in addition to its previously known function during cytokinesis.
Title: Centralspindlin is required for thorax development during Drosophila metamorphosis.

Data show that Sticky (Sti), tile ring component Citron kinase (CIT-K) interacts directly with two kinesins, Nebbish and Pavarotti and that in turn these kinesins interact with each other and with another central spindle protein, Fascetto.
Title: Citron kinase controls a molecular network required for midbody formation in cytokinesis.

The following pav gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pav cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17488	NM_057677.4 Latest version!	Drosophila melanogaster pavarotti (pav), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pav ( NM_057677.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477025.1 Drosophila melanogaster pavarotti (pav), mRNA.

Price & Availability↑

CloneID	OFa17488
Clone ID Related Accession (Same CDS sequence)	NM_057677.4
Accession Version	NM_057677.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pavarotti
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_057677.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGGCAG TACCCAGGAC GCCGATGCGT GTCGTCCCGA AAACTCCGCG GGTGCATCAA 
ACGGTGGAGA AACAGCGACG AGACACCTCC GACAAGGCCA GGGATCCAGT GAATGTGTTC 
TGTCGAGTGC GGCCACTACA ATCCGACGCA GATCTCACCT CCCTAAGGGT CAAGAACTCC 
ACGACGATCG CACTGAATCC GCAGGATCAG CTGCTGCAGC ATCACAAGCC ACACAACGGC 
GCCCAGCGCG AGGTTCAGTA CATATTCAAG CACGTTTTCC AGCCGGATGC CACGCAGCAG 
GATGTGTACG CAGCTGTGGC CCAGCCGTTG GTGGAGAACC TACTGAAAGG CCGAAACAGT 
CTGCTTTTCA CCTACGGCGT GACTGGAAGT GGAAAAACGT ACACCATGAC CGGCAACCTG 
CGACACCGCG GCATCATGCC GCGCTGCCTG GACGTGCTCT TTCGCACCAT TTCAGATTAC 
CAGGCCAAGA AGTTCGTCTT CAAGCCGGAC AAGCTTAATG GCTTCGAGAT CCTGTCCGAA 
GAGGATGCGC TTTTGGAGCG CCAACACGAG ATGAACCAGC GTTTTGCTGG CTCAGGACGA 
TTTGCCTTCA GGCACAAGGA CTCGGATCCG GAAATTGCAT CCCAGGCCTC CGTGGAGCCA 
ATTCCGCTGC TGGGTTTGGA TGAGGACAAC ATGTACTCGG TGTTTGTCAC CTATATTGAA 
ATTTACAATA ACAGCGTCTA CGACCTCCTT GAGGACTCGG GTATACAGAA GACTTTGCAG 
AGCAAAATCA TTCGTGAAGA TGCCAATCGA CACATGTTCG TCCACGGCGT CACAGAGGTG 
GAGGTCAAGA CCGTGGAGGA CGCCCTCGAG GTCTTTCAAA TGGGCCAGAA GCGCAAACGA 
ATGGGACACA CTGTTCTCAA TGCAGAATCC AGTAGGAGTC ACTCGGTGTT CAATATACGC 
TTGGTGCAGG CGCCCACTGA TAGCCAGGGT GAGAATGTGG TTCAGGACCG GCAAAACATC 
ACAGTGAGTC AACTGTCCCT GGTGGATCTG GCGGGCAGTG AGCGATCCTC AAGGACCAAG 
AACACCGGTG TGCGACTCCG TGAGGCGGGC AACATCAATA ATTCGCTGAT GACGCTGCGC 
ACTTGCCTGG AATATCTGCG CGAGAACCAA CTCGCGGCTA GCAATGGATT GGCACCCAAG 
AAGGTCCCCT ATCGAGACTC CAAGATCACA CACATGTTTA AAAACTACTT CGATGGCGAA 
GGCCAGGTGT CCATGATTGT GTGCATCAAC CCAAGAATTG AGGACTATGA TGAGAATATG 
CAAGTGATGA AGTTCGCCGA GATGACCCAG GAGGTGCAAA TTGCTCGGGC GACTCCTATG 
AAGCAAGATT TGGGTCTGAC TCCCGGCCGT CGCAAGGCCA ACAAGCTGTT CAAGATTGCC 
GTTAATAATC TAAACGAGCT GGGCATTCCT GAGGCCAAGG ATTTGGAGGT GGACGTGGGT 
TTGGTGTACA GTTTGGGACC CGATTTTCCC GCCTACGAAA TGGACAGTCC CGAGGCACAG 
ATCAAAATCC GCGAGCTAAT GCATTATCTG GAGCAGCGCA TAGAAAAGCG AAAGAAGCTG 
CGTGCCAACT TGGACATTAA ATGTGACAGC TTCCGGCAGA TGCTGATGAA CCTTGACCGC 
GACAATCTTC AGCTGCGCAC GGAGCTCGCC TCCTTAAAGG CTGTTTACAA GCAGGAGCGC 
GATCGCAGTG CGGCACTCGA GAAGAAGGTT CGCATCCACG AGAGCTCCAT TGACGTGCTT 
AACAATACGC TAAGCAAGCA AGAACGGCAG ATTGAAGAGC TGACTTTCAA GCTGAACGAG 
AAGGAGAATA TGCTCACCCA AAAGGAGCAC GAGAAGGAAA AGCAAAAGAA GAAGTTCAGC 
TCCAAGCTGG CCGTTGAGTC GGACAAAAAT AAGCGAGAGT TTGAACTAAA GCTGCGTGAA 
CAGCGAGTGA AATTGCAGGA ACGCATGCGC ATCAAGGACG AGAAGTTGCG ATTGGTCTCC 
AACATTCTGC AGTCCGAAGA TCTGCCCAGT TTGCCGCGGT CACAGAGCTC GGAAAACATA 
CTTAATGAGA AAGATCGCGG TGCATATACG GCGCGCACGG AGGAGTCCTC GGTGCCAGCT 
ACACGAACAG ATATATATGC AACGCCACGT CATGGTGCAG CAGCGGCCAA CAATCGGCAT 
AGACGCTCTC GATCCGCAGG CGACAAGTGG CTGGAGCATC GGGCAGCTAA TCCCGTTCCG 
CTTGGCACTA TTATGCAGCC GTATCTGAAG AATCGCAAAT CCGTAACGAA ACTAACCGAC 
TTGAAGGAGT TGACTGCTCA CGGAACTACG AAGTACTGTC TCGTCTCGCA GGATGCGGAT 
ACGGATGGCG ATGTGGAGAC GAAGCTGTAT AAGGGCAATG TAATTCCCAC TTGTGGCGGT 
GGTGCCCAAG TTGTGTTCAA CGATGTGGAG TGCCTCAAGC AGAAGTCACC GGTTCACTCG 
CCCACGCGAA AGCGACCCAG CAATGGCAAC ATTTCGGCTC TGGGCGGCGG TGCAGTGCCC 
AGCACGGTCA CCTCAGCCCA GGACGTAGCC TCCCGCTGCA ATCTCGGCAT TGAGGGACAC 
AGCAGCAAGA AGTCGAAAAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477025.1
CDS	145..2808
Translation	MKAVPRTPMRVVPKTPRVHQTVEKQRRDTSDKARDPVNVFCRVRPLQSDADLTSLRVKNSTTIALNPQDQLLQHHKPHNGAQREVQYIFKHVFQPDATQQDVYAAVAQPLVENLLKGRNSLLFTYGVTGSGKTYTMTGNLRHRGIMPRCLDVLFRTISDYQAKKFVFKPDKLNGFEILSEEDALLERQHEMNQRFAGSGRFAFRHKDSDPEIASQASVEPIPLLGLDEDNMYSVFVTYIEIYNNSVYDLLEDSGIQKTLQSKIIREDANRHMFVHGVTEVEVKTVEDALEVFQMGQKRKRMGHTVLNAESSRSHSVFNIRLVQAPTDSQGENVVQDRQNITVSQLSLVDLAGSERSSRTKNTGVRLREAGNINNSLMTLRTCLEYLRENQLAASNGLAPKKVPYRDSKITHMFKNYFDGEGQVSMIVCINPRIEDYDENMQVMKFAEMTQEVQIARATPMKQDLGLTPGRRKANKLFKIAVNNLNELGIPEAKDLEVDVGLVYSLGPDFPAYEMDSPEAQIKIRELMHYLEQRIEKRKKLRANLDIKCDSFRQMLMNLDRDNLQLRTELASLKAVYKQERDRSAALEKKVRIHESSIDVLNNTLSKQERQIEELTFKLNEKENMLTQKEHEKEKQKKKFSSKLAVESDKNKREFELKLREQRVKLQERMRIKDEKLRLVSNILQSEDLPSLPRSQSSENILNEKDRGAYTARTEESSVPATRTDIYATPRHGAAAANNRHRRSRSAGDKWLEHRAANPVPLGTIMQPYLKNRKSVTKLTDLKELTAHGTTKYCLVSQDADTDGDVETKLYKGNVIPTCGGGAQVVFNDVECLKQKSPVHSPTRKRPSNGNISALGGGAVPSTVTSAQDVASRCNLGIEGHSSKKSKI

Target ORF information:

RefSeq Version	NM_057677.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pavarotti (pav), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057677.4

ORF Insert Sequence:

ATGAAGGCAG TACCCAGGAC GCCGATGCGT GTCGTCCCGA AAACTCCGCG GGTGCATCAA 
ACGGTGGAGA AACAGCGACG AGACACCTCC GACAAGGCCA GGGATCCAGT GAATGTGTTC 
TGTCGAGTGC GGCCACTACA ATCCGACGCA GATCTCACCT CCCTAAGGGT CAAGAACTCC 
ACGACGATCG CACTGAATCC GCAGGATCAG CTGCTGCAGC ATCACAAGCC ACACAACGGC 
GCCCAGCGCG AGGTTCAGTA CATATTCAAG CACGTTTTCC AGCCGGATGC CACGCAGCAG 
GATGTGTACG CAGCTGTGGC CCAGCCGTTG GTGGAGAACC TACTGAAAGG CCGAAACAGT 
CTGCTTTTCA CCTACGGCGT GACTGGAAGT GGAAAAACGT ACACCATGAC CGGCAACCTG 
CGACACCGCG GCATCATGCC GCGCTGCCTG GACGTGCTCT TTCGCACCAT TTCAGATTAC 
CAGGCCAAGA AGTTCGTCTT CAAGCCGGAC AAGCTTAATG GCTTCGAGAT CCTGTCCGAA 
GAGGATGCGC TTTTGGAGCG CCAACACGAG ATGAACCAGC GTTTTGCTGG CTCAGGACGA 
TTTGCCTTCA GGCACAAGGA CTCGGATCCG GAAATTGCAT CCCAGGCCTC CGTGGAGCCA 
ATTCCGCTGC TGGGTTTGGA TGAGGACAAC ATGTACTCGG TGTTTGTCAC CTATATTGAA 
ATTTACAATA ACAGCGTCTA CGACCTCCTT GAGGACTCGG GTATACAGAA GACTTTGCAG 
AGCAAAATCA TTCGTGAAGA TGCCAATCGA CACATGTTCG TCCACGGCGT CACAGAGGTG 
GAGGTCAAGA CCGTGGAGGA CGCCCTCGAG GTCTTTCAAA TGGGCCAGAA GCGCAAACGA 
ATGGGACACA CTGTTCTCAA TGCAGAATCC AGTAGGAGTC ACTCGGTGTT CAATATACGC 
TTGGTGCAGG CGCCCACTGA TAGCCAGGGT GAGAATGTGG TTCAGGACCG GCAAAACATC 
ACAGTGAGTC AACTGTCCCT GGTGGATCTG GCGGGCAGTG AGCGATCCTC AAGGACCAAG 
AACACCGGTG TGCGACTCCG TGAGGCGGGC AACATCAATA ATTCGCTGAT GACGCTGCGC 
ACTTGCCTGG AATATCTGCG CGAGAACCAA CTCGCGGCTA GCAATGGATT GGCACCCAAG 
AAGGTCCCCT ATCGAGACTC CAAGATCACA CACATGTTTA AAAACTACTT CGATGGCGAA 
GGCCAGGTGT CCATGATTGT GTGCATCAAC CCAAGAATTG AGGACTATGA TGAGAATATG 
CAAGTGATGA AGTTCGCCGA GATGACCCAG GAGGTGCAAA TTGCTCGGGC GACTCCTATG 
AAGCAAGATT TGGGTCTGAC TCCCGGCCGT CGCAAGGCCA ACAAGCTGTT CAAGATTGCC 
GTTAATAATC TAAACGAGCT GGGCATTCCT GAGGCCAAGG ATTTGGAGGT GGACGTGGGT 
TTGGTGTACA GTTTGGGACC CGATTTTCCC GCCTACGAAA TGGACAGTCC CGAGGCACAG 
ATCAAAATCC GCGAGCTAAT GCATTATCTG GAGCAGCGCA TAGAAAAGCG AAAGAAGCTG 
CGTGCCAACT TGGACATTAA ATGTGACAGC TTCCGGCAGA TGCTGATGAA CCTTGACCGC 
GACAATCTTC AGCTGCGCAC GGAGCTCGCC TCCTTAAAGG CTGTTTACAA GCAGGAGCGC 
GATCGCAGTG CGGCACTCGA GAAGAAGGTT CGCATCCACG AGAGCTCCAT TGACGTGCTT 
AACAATACGC TAAGCAAGCA AGAACGGCAG ATTGAAGAGC TGACTTTCAA GCTGAACGAG 
AAGGAGAATA TGCTCACCCA AAAGGAGCAC GAGAAGGAAA AGCAAAAGAA GAAGTTCAGC 
TCCAAGCTGG CCGTTGAGTC GGACAAAAAT AAGCGAGAGT TTGAACTAAA GCTGCGTGAA 
CAGCGAGTGA AATTGCAGGA ACGCATGCGC ATCAAGGACG AGAAGTTGCG ATTGGTCTCC 
AACATTCTGC AGTCCGAAGA TCTGCCCAGT TTGCCGCGGT CACAGAGCTC GGAAAACATA 
CTTAATGAGA AAGATCGCGG TGCATATACG GCGCGCACGG AGGAGTCCTC GGTGCCAGCT 
ACACGAACAG ATATATATGC AACGCCACGT CATGGTGCAG CAGCGGCCAA CAATCGGCAT 
AGACGCTCTC GATCCGCAGG CGACAAGTGG CTGGAGCATC GGGCAGCTAA TCCCGTTCCG 
CTTGGCACTA TTATGCAGCC GTATCTGAAG AATCGCAAAT CCGTAACGAA ACTAACCGAC 
TTGAAGGAGT TGACTGCTCA CGGAACTACG AAGTACTGTC TCGTCTCGCA GGATGCGGAT 
ACGGATGGCG ATGTGGAGAC GAAGCTGTAT AAGGGCAATG TAATTCCCAC TTGTGGCGGT 
GGTGCCCAAG TTGTGTTCAA CGATGTGGAG TGCCTCAAGC AGAAGTCACC GGTTCACTCG 
CCCACGCGAA AGCGACCCAG CAATGGCAAC ATTTCGGCTC TGGGCGGCGG TGCAGTGCCC 
AGCACGGTCA CCTCAGCCCA GGACGTAGCC TCCCGCTGCA ATCTCGGCAT TGAGGGACAC 
AGCAGCAAGA AGTCGAAAAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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pav cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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