Ubi-p63E cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ubi-p63E
Entrez Gene ID	38456
Full Name	Ubiquitin-63E
Synonyms	CG11624,DmUb,DmUbi-p63E,Dmel\CG11624,UB,Ub,Ub-p63E,Ubi-P63e,Ubi-p,Ubi63E,Ubi63p,Ubip,Ubip63E,Ubiq,Ubp,magn,ubi,ubi-p63E,ubi63E
General protein information	Preferred Names Ubiquitin-63E Names CG11624 gene product from transcript CG11624-RB CG11624-PA CG11624-PB CG11624-PC CG11624-PD Ubi-p63E-PA Ubi-p63E-PB Ubi-p63E-PC Ubi-p63E-PD Ubiquitin-63-E Ubiquitin-polymeric magellan polyubiquitin ubiquitin ubiquitin 63E
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 63F5-63F5

mRNA and Protein(s)

mRNA	Protein	Name
NM_168043.3	NP_728908.1	Ubiquitin-63E, isoform A
NM_206272.1	NP_995994.1	Ubiquitin-63E, isoform C
NM_079185.4	NP_523909.2	Ubiquitin-63E, isoform B
NM_001274454.1	NP_001261383.1	Ubiquitin-63E, isoform D

Pathways from BioSystems

Homologies

	LOC712934	XP_002798876.1
Homo sapiens (human)	UBC	NP_066289.2
Bos taurus (cattle)	UBC	NP_001193236.1
Drosophila melanogaster (fruit fly)	Ubi-p63E	NP_001261383.1
Arabidopsis thaliana (thale cress)	UBQ8	NP_566357.1
Pan troglodytes (chimpanzee)	UBC	NP_001129078.1
Mus musculus (house mouse)	Ubc	NP_062613.3
Rattus norvegicus (Norway rat)	Ubc	NP_059010.1
Canis lupus familiaris (dog)	UBC	XP_853060.1
Xenopus tropicalis (tropical clawed frog)	ubb	XP_004911738.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results showed the molecular cloning UB and L40 genes from Drosophila kc cells and the first evidence that both of them play roles in the regulation of deltamethrin resistance.
Title: Analysis of UB and L40 resistance related to deltamethrin in Drosophila kc cells.

The polyubiquitin gene Ubi-p63E is essential for male meiotic cell cycle progression and germ cell differentiation in Drosophila.
Title: The polyubiquitin gene Ubi-p63E is essential for male meiotic cell cycle progression and germ cell differentiation in Drosophila.

Ubiquitylation of p54
Title: Ubiquitylation of Drosophila p54/Rpn10/S5a regulates its interaction with the UBA-UBL polyubiquitin receptors.

The following Ubi-p63E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ubi-p63E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17429	NM_001274454.1 Latest version!	Drosophila melanogaster Ubiquitin-63E (Ubi-p63E), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa17429	NM_168043.3 Latest version!	Drosophila melanogaster Ubiquitin-63E (Ubi-p63E), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa17429	NM_079185.4 Latest version!	Drosophila melanogaster Ubiquitin-63E (Ubi-p63E), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa17429	NM_206272.1 Latest version!	Drosophila melanogaster Ubiquitin-63E (Ubi-p63E), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ubi-p63E ( NM_168043.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728908.1 Drosophila melanogaster Ubiquitin-63E (Ubi-p63E), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa17429
Clone ID Related Accession (Same CDS sequence)	NM_168043.3 , NM_206272.1 , NM_079185.4 , NM_001274454.1
Accession Version	NM_168043.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ubiquitin-63E, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168043.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGATCT TTGTGAAGAC TTTGACCGGA AAGACCATCA CCCTCGAGGT AGAGCCCTCG 
GACACCATTG AGAATGTTAA GGCTAAGATC CAAGACAAGG AAGGAATTCC CCCAGATCAG 
CAGCGTCTGA TCTTCGCCGG CAAGCAACTG GAAGACGGAC GCACCCTGTC CGATTACAAC 
ATTCAGAAGG AGTCCACCCT TCACTTGGTC CTTCGTCTCC GTGGTGGTAT GCAGATCTTC 
GTGAAGACTT TGACCGGAAA GACCATCACC CTCGAGGTAG AGCCATCGGA CACCATTGAG 
AATGTGAAGG CCAAGATTCA GGATAAGGAG GGAATCCCCC CAGATCAGCA GCGTCTGATC 
TTCGCTGGCA AGCAGCTTGA GGATGGACGC ACCCTGTCCG ATTACAACAT CCAGAAGGAG 
TCCACCCTTC ACTTGGTCCT TCGTCTCCGT GGTGGTATGC AGATCTTCGT GAAGACTTTG 
ACCGGAAAGA CCATCACCCT CGAGGTAGAG CCATCGGACA CAATTGAGAA TGTTAAGGCT 
AAGATCCAAG ACAAGGAGGG AATTCCCCCA GATCAGCAGC GTCTGATCTT CGCCGGCAAG 
CAGCTTGAGG ATGGACGCAC CCTGTCCGAT TACAACATCC AGAAGGAGTC CACCCTTCAC 
TTGGTCCTTC GTCTCCGTGG TGGTATGCAG ATCTTCGTGA AGACTTTGAC CGGAAAGACC 
ATCACCCTCG AGGTAGAGCC TTCGGACACC ATTGAGAATG TTAAGGCTAA GATCCAAGAC 
AAGGAGGGAA TTCCCCCAGA TCAGCAGCGT CTGATCTTCG CTGGCAAGCA GCTTGAGGAT 
GGACGCACCC TGTCCGATTA CAACATTCAG AAGGAGTCCA CCCTTCACTT GGTCCTTCGT 
CTTCGTGGTG GTATGCAGAT CTTCGTGAAG ACTTTGACCG GAAAGACCAT CACCCTCGAG 
GTAGAGCCAT CGGACACAAT TGAGAATGTT AAGGCTAAGA TCCAAGACAA GGAGGGAATT 
CCCCCAGATC AGCAGCGTCT GATCTTCGCC GGCAAGCAGC TTGAGGATGG ACGCACCCTG 
TCCGATTACA ACATCCAGAA GGAGTCCACC CTTCACTTGG TCCTTCGTCT CCGTGGTGGT 
ATGCAGATCT TCGTGAAGAC TTTGACCGGA AAGACCATCA CCCTCGAGGT AGAGCCATCG 
GACACCATTG AGAATGTGAA GGCCAAGATT CAGGATAAGG AGGGAATCCC CCCAGATCAG 
CAGCGTCTGA TCTTCGCCGG CAAGCAGCTT GAGGATGGAC GCACCCTGTC CGATTACAAC 
ATCCAGAAGG AGTCCACCCT TCACTTGGTC CTTCGTCTCC GTGGTGGTAT GCAGATCTTC 
GTGAAGACTT TGACCGGAAA GACCATCACC CTCGAGGTAG AGCCTTCGGA CACCATTGAG 
AATGTTAAGG CTAAGATCCA AGACAAGGAG GGAATTCCCC CAGATCAGCA GCGTCTGATC 
TTCGCCGGCA AGCAGCTTGA GGATGGACGC ACCCTGTCCG ATTACAACAT CCAGAAGGAG 
TCCACCCTTC ACTTGGTCCT TCGTCTTCGT GGTGGTATGC AGATCTTCGT GAAGACTTTG 
ACCGGAAAGA CCATCACCCT CGAGGTAGAG CCATCGGACA CAATTGAGAA TGTTAAGGCT 
AAGATCCAAG ACAAGGAGGG AATTCCCCCA GATCAGCAGC GTCTGATCTT CGCCGGCAAG 
CAGCTTGAGG ATGGACGCAC CCTGTCCGAT TACAACATCC AGAAGGAGTC CACCCTTCAC 
TTGGTCCTTC GTCTCCGTGG TGGTATGCAG ATCTTCGTGA AGACTTTGAC CGGAAAGACC 
ATCACCCTCG AGGTAGAGCC TTCGGACACC ATTGAGAATG TTAAGGCTAA GATCCAAGAC 
AAGGAGGGAA TTCCCCCAGA TCAGCAGCGT CTGATCTTCG CCGGCAAGCA GCTTGAGGAT 
GGACGCACCC TGTCCGATTA CAACATCCAG AAGGAGTCCA CCCTTCACTT GGTCCTTCGT 
CTCCGTGGTG GTATGCAGAT CTTCGTGAAG ACTTTGACCG GAAAGACCAT CACCCTCGAG 
GTAGAGCCCT CGGACACCAT TGAGAATGTT AAGGCTAAGA TCCAAGACAA GGAGGGAATT 
CCCCCAGATC AGCAGCGTCT GATCTTCGCC GGCAAGCAGC TTGAGGATGG ACGCACCCTG 
TCCGATTACA ACATCCAGAA GGAATCGACC CTTCACTTGG TGCTCCGTCT CCGTGGAGGA 
ATCCAGGCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728908.1
CDS	193..2484
Translation	MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGIQA

Target ORF information:

RefSeq Version	NM_168043.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ubiquitin-63E (Ubi-p63E), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168043.3

ORF Insert Sequence:

ATGCAGATCT TTGTGAAGAC TTTGACCGGA AAGACCATCA CCCTCGAGGT AGAGCCCTCG 
GACACCATTG AGAATGTTAA GGCTAAGATC CAAGACAAGG AAGGAATTCC CCCAGATCAG 
CAGCGTCTGA TCTTCGCCGG CAAGCAACTG GAAGACGGAC GCACCCTGTC CGATTACAAC 
ATTCAGAAGG AGTCCACCCT TCACTTGGTC CTTCGTCTCC GTGGTGGTAT GCAGATCTTC 
GTGAAGACTT TGACCGGAAA GACCATCACC CTCGAGGTAG AGCCATCGGA CACCATTGAG 
AATGTGAAGG CCAAGATTCA GGATAAGGAG GGAATCCCCC CAGATCAGCA GCGTCTGATC 
TTCGCTGGCA AGCAGCTTGA GGATGGACGC ACCCTGTCCG ATTACAACAT CCAGAAGGAG 
TCCACCCTTC ACTTGGTCCT TCGTCTCCGT GGTGGTATGC AGATCTTCGT GAAGACTTTG 
ACCGGAAAGA CCATCACCCT CGAGGTAGAG CCATCGGACA CAATTGAGAA TGTTAAGGCT 
AAGATCCAAG ACAAGGAGGG AATTCCCCCA GATCAGCAGC GTCTGATCTT CGCCGGCAAG 
CAGCTTGAGG ATGGACGCAC CCTGTCCGAT TACAACATCC AGAAGGAGTC CACCCTTCAC 
TTGGTCCTTC GTCTCCGTGG TGGTATGCAG ATCTTCGTGA AGACTTTGAC CGGAAAGACC 
ATCACCCTCG AGGTAGAGCC TTCGGACACC ATTGAGAATG TTAAGGCTAA GATCCAAGAC 
AAGGAGGGAA TTCCCCCAGA TCAGCAGCGT CTGATCTTCG CTGGCAAGCA GCTTGAGGAT 
GGACGCACCC TGTCCGATTA CAACATTCAG AAGGAGTCCA CCCTTCACTT GGTCCTTCGT 
CTTCGTGGTG GTATGCAGAT CTTCGTGAAG ACTTTGACCG GAAAGACCAT CACCCTCGAG 
GTAGAGCCAT CGGACACAAT TGAGAATGTT AAGGCTAAGA TCCAAGACAA GGAGGGAATT 
CCCCCAGATC AGCAGCGTCT GATCTTCGCC GGCAAGCAGC TTGAGGATGG ACGCACCCTG 
TCCGATTACA ACATCCAGAA GGAGTCCACC CTTCACTTGG TCCTTCGTCT CCGTGGTGGT 
ATGCAGATCT TCGTGAAGAC TTTGACCGGA AAGACCATCA CCCTCGAGGT AGAGCCATCG 
GACACCATTG AGAATGTGAA GGCCAAGATT CAGGATAAGG AGGGAATCCC CCCAGATCAG 
CAGCGTCTGA TCTTCGCCGG CAAGCAGCTT GAGGATGGAC GCACCCTGTC CGATTACAAC 
ATCCAGAAGG AGTCCACCCT TCACTTGGTC CTTCGTCTCC GTGGTGGTAT GCAGATCTTC 
GTGAAGACTT TGACCGGAAA GACCATCACC CTCGAGGTAG AGCCTTCGGA CACCATTGAG 
AATGTTAAGG CTAAGATCCA AGACAAGGAG GGAATTCCCC CAGATCAGCA GCGTCTGATC 
TTCGCCGGCA AGCAGCTTGA GGATGGACGC ACCCTGTCCG ATTACAACAT CCAGAAGGAG 
TCCACCCTTC ACTTGGTCCT TCGTCTTCGT GGTGGTATGC AGATCTTCGT GAAGACTTTG 
ACCGGAAAGA CCATCACCCT CGAGGTAGAG CCATCGGACA CAATTGAGAA TGTTAAGGCT 
AAGATCCAAG ACAAGGAGGG AATTCCCCCA GATCAGCAGC GTCTGATCTT CGCCGGCAAG 
CAGCTTGAGG ATGGACGCAC CCTGTCCGAT TACAACATCC AGAAGGAGTC CACCCTTCAC 
TTGGTCCTTC GTCTCCGTGG TGGTATGCAG ATCTTCGTGA AGACTTTGAC CGGAAAGACC 
ATCACCCTCG AGGTAGAGCC TTCGGACACC ATTGAGAATG TTAAGGCTAA GATCCAAGAC 
AAGGAGGGAA TTCCCCCAGA TCAGCAGCGT CTGATCTTCG CCGGCAAGCA GCTTGAGGAT 
GGACGCACCC TGTCCGATTA CAACATCCAG AAGGAGTCCA CCCTTCACTT GGTCCTTCGT 
CTCCGTGGTG GTATGCAGAT CTTCGTGAAG ACTTTGACCG GAAAGACCAT CACCCTCGAG 
GTAGAGCCCT CGGACACCAT TGAGAATGTT AAGGCTAAGA TCCAAGACAA GGAGGGAATT 
CCCCCAGATC AGCAGCGTCT GATCTTCGCC GGCAAGCAGC TTGAGGATGG ACGCACCCTG 
TCCGATTACA ACATCCAGAA GGAATCGACC CTTCACTTGG TGCTCCGTCT CCGTGGAGGA 
ATCCAGGCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ubi-p63E ( NM_206272.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995994.1 Drosophila melanogaster Ubiquitin-63E (Ubi-p63E), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa17429
Clone ID Related Accession (Same CDS sequence)	NM_168043.3 , NM_206272.1 , NM_079185.4 , NM_001274454.1
Accession Version	NM_206272.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ubiquitin-63E, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGATCT TTGTGAAGAC TTTGACCGGA AAGACCATCA CCCTCGAGGT AGAGCCCTCG 
GACACCATTG AGAATGTTAA GGCTAAGATC CAAGACAAGG AAGGAATTCC CCCAGATCAG 
CAGCGTCTGA TCTTCGCCGG CAAGCAACTG GAAGACGGAC GCACCCTGTC CGATTACAAC 
ATTCAGAAGG AGTCCACCCT TCACTTGGTC CTTCGTCTCC GTGGTGGTAT GCAGATCTTC 
GTGAAGACTT TGACCGGAAA GACCATCACC CTCGAGGTAG AGCCATCGGA CACCATTGAG 
AATGTGAAGG CCAAGATTCA GGATAAGGAG GGAATCCCCC CAGATCAGCA GCGTCTGATC 
TTCGCTGGCA AGCAGCTTGA GGATGGACGC ACCCTGTCCG ATTACAACAT CCAGAAGGAG 
TCCACCCTTC ACTTGGTCCT TCGTCTCCGT GGTGGTATGC AGATCTTCGT GAAGACTTTG 
ACCGGAAAGA CCATCACCCT CGAGGTAGAG CCATCGGACA CAATTGAGAA TGTTAAGGCT 
AAGATCCAAG ACAAGGAGGG AATTCCCCCA GATCAGCAGC GTCTGATCTT CGCCGGCAAG 
CAGCTTGAGG ATGGACGCAC CCTGTCCGAT TACAACATCC AGAAGGAGTC CACCCTTCAC 
TTGGTCCTTC GTCTCCGTGG TGGTATGCAG ATCTTCGTGA AGACTTTGAC CGGAAAGACC 
ATCACCCTCG AGGTAGAGCC TTCGGACACC ATTGAGAATG TTAAGGCTAA GATCCAAGAC 
AAGGAGGGAA TTCCCCCAGA TCAGCAGCGT CTGATCTTCG CTGGCAAGCA GCTTGAGGAT 
GGACGCACCC TGTCCGATTA CAACATTCAG AAGGAGTCCA CCCTTCACTT GGTCCTTCGT 
CTTCGTGGTG GTATGCAGAT CTTCGTGAAG ACTTTGACCG GAAAGACCAT CACCCTCGAG 
GTAGAGCCAT CGGACACAAT TGAGAATGTT AAGGCTAAGA TCCAAGACAA GGAGGGAATT 
CCCCCAGATC AGCAGCGTCT GATCTTCGCC GGCAAGCAGC TTGAGGATGG ACGCACCCTG 
TCCGATTACA ACATCCAGAA GGAGTCCACC CTTCACTTGG TCCTTCGTCT CCGTGGTGGT 
ATGCAGATCT TCGTGAAGAC TTTGACCGGA AAGACCATCA CCCTCGAGGT AGAGCCATCG 
GACACCATTG AGAATGTGAA GGCCAAGATT CAGGATAAGG AGGGAATCCC CCCAGATCAG 
CAGCGTCTGA TCTTCGCCGG CAAGCAGCTT GAGGATGGAC GCACCCTGTC CGATTACAAC 
ATCCAGAAGG AGTCCACCCT TCACTTGGTC CTTCGTCTCC GTGGTGGTAT GCAGATCTTC 
GTGAAGACTT TGACCGGAAA GACCATCACC CTCGAGGTAG AGCCTTCGGA CACCATTGAG 
AATGTTAAGG CTAAGATCCA AGACAAGGAG GGAATTCCCC CAGATCAGCA GCGTCTGATC 
TTCGCCGGCA AGCAGCTTGA GGATGGACGC ACCCTGTCCG ATTACAACAT CCAGAAGGAG 
TCCACCCTTC ACTTGGTCCT TCGTCTTCGT GGTGGTATGC AGATCTTCGT GAAGACTTTG 
ACCGGAAAGA CCATCACCCT CGAGGTAGAG CCATCGGACA CAATTGAGAA TGTTAAGGCT 
AAGATCCAAG ACAAGGAGGG AATTCCCCCA GATCAGCAGC GTCTGATCTT CGCCGGCAAG 
CAGCTTGAGG ATGGACGCAC CCTGTCCGAT TACAACATCC AGAAGGAGTC CACCCTTCAC 
TTGGTCCTTC GTCTCCGTGG TGGTATGCAG ATCTTCGTGA AGACTTTGAC CGGAAAGACC 
ATCACCCTCG AGGTAGAGCC TTCGGACACC ATTGAGAATG TTAAGGCTAA GATCCAAGAC 
AAGGAGGGAA TTCCCCCAGA TCAGCAGCGT CTGATCTTCG CCGGCAAGCA GCTTGAGGAT 
GGACGCACCC TGTCCGATTA CAACATCCAG AAGGAGTCCA CCCTTCACTT GGTCCTTCGT 
CTCCGTGGTG GTATGCAGAT CTTCGTGAAG ACTTTGACCG GAAAGACCAT CACCCTCGAG 
GTAGAGCCCT CGGACACCAT TGAGAATGTT AAGGCTAAGA TCCAAGACAA GGAGGGAATT 
CCCCCAGATC AGCAGCGTCT GATCTTCGCC GGCAAGCAGC TTGAGGATGG ACGCACCCTG 
TCCGATTACA ACATCCAGAA GGAATCGACC CTTCACTTGG TGCTCCGTCT CCGTGGAGGA 
ATCCAGGCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995994.1
CDS	210..2501
Translation	MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGIQA

Target ORF information:

RefSeq Version	NM_206272.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ubiquitin-63E (Ubi-p63E), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206272.1

ORF Insert Sequence:

ATGCAGATCT TTGTGAAGAC TTTGACCGGA AAGACCATCA CCCTCGAGGT AGAGCCCTCG 
GACACCATTG AGAATGTTAA GGCTAAGATC CAAGACAAGG AAGGAATTCC CCCAGATCAG 
CAGCGTCTGA TCTTCGCCGG CAAGCAACTG GAAGACGGAC GCACCCTGTC CGATTACAAC 
ATTCAGAAGG AGTCCACCCT TCACTTGGTC CTTCGTCTCC GTGGTGGTAT GCAGATCTTC 
GTGAAGACTT TGACCGGAAA GACCATCACC CTCGAGGTAG AGCCATCGGA CACCATTGAG 
AATGTGAAGG CCAAGATTCA GGATAAGGAG GGAATCCCCC CAGATCAGCA GCGTCTGATC 
TTCGCTGGCA AGCAGCTTGA GGATGGACGC ACCCTGTCCG ATTACAACAT CCAGAAGGAG 
TCCACCCTTC ACTTGGTCCT TCGTCTCCGT GGTGGTATGC AGATCTTCGT GAAGACTTTG 
ACCGGAAAGA CCATCACCCT CGAGGTAGAG CCATCGGACA CAATTGAGAA TGTTAAGGCT 
AAGATCCAAG ACAAGGAGGG AATTCCCCCA GATCAGCAGC GTCTGATCTT CGCCGGCAAG 
CAGCTTGAGG ATGGACGCAC CCTGTCCGAT TACAACATCC AGAAGGAGTC CACCCTTCAC 
TTGGTCCTTC GTCTCCGTGG TGGTATGCAG ATCTTCGTGA AGACTTTGAC CGGAAAGACC 
ATCACCCTCG AGGTAGAGCC TTCGGACACC ATTGAGAATG TTAAGGCTAA GATCCAAGAC 
AAGGAGGGAA TTCCCCCAGA TCAGCAGCGT CTGATCTTCG CTGGCAAGCA GCTTGAGGAT 
GGACGCACCC TGTCCGATTA CAACATTCAG AAGGAGTCCA CCCTTCACTT GGTCCTTCGT 
CTTCGTGGTG GTATGCAGAT CTTCGTGAAG ACTTTGACCG GAAAGACCAT CACCCTCGAG 
GTAGAGCCAT CGGACACAAT TGAGAATGTT AAGGCTAAGA TCCAAGACAA GGAGGGAATT 
CCCCCAGATC AGCAGCGTCT GATCTTCGCC GGCAAGCAGC TTGAGGATGG ACGCACCCTG 
TCCGATTACA ACATCCAGAA GGAGTCCACC CTTCACTTGG TCCTTCGTCT CCGTGGTGGT 
ATGCAGATCT TCGTGAAGAC TTTGACCGGA AAGACCATCA CCCTCGAGGT AGAGCCATCG 
GACACCATTG AGAATGTGAA GGCCAAGATT CAGGATAAGG AGGGAATCCC CCCAGATCAG 
CAGCGTCTGA TCTTCGCCGG CAAGCAGCTT GAGGATGGAC GCACCCTGTC CGATTACAAC 
ATCCAGAAGG AGTCCACCCT TCACTTGGTC CTTCGTCTCC GTGGTGGTAT GCAGATCTTC 
GTGAAGACTT TGACCGGAAA GACCATCACC CTCGAGGTAG AGCCTTCGGA CACCATTGAG 
AATGTTAAGG CTAAGATCCA AGACAAGGAG GGAATTCCCC CAGATCAGCA GCGTCTGATC 
TTCGCCGGCA AGCAGCTTGA GGATGGACGC ACCCTGTCCG ATTACAACAT CCAGAAGGAG 
TCCACCCTTC ACTTGGTCCT TCGTCTTCGT GGTGGTATGC AGATCTTCGT GAAGACTTTG 
ACCGGAAAGA CCATCACCCT CGAGGTAGAG CCATCGGACA CAATTGAGAA TGTTAAGGCT 
AAGATCCAAG ACAAGGAGGG AATTCCCCCA GATCAGCAGC GTCTGATCTT CGCCGGCAAG 
CAGCTTGAGG ATGGACGCAC CCTGTCCGAT TACAACATCC AGAAGGAGTC CACCCTTCAC 
TTGGTCCTTC GTCTCCGTGG TGGTATGCAG ATCTTCGTGA AGACTTTGAC CGGAAAGACC 
ATCACCCTCG AGGTAGAGCC TTCGGACACC ATTGAGAATG TTAAGGCTAA GATCCAAGAC 
AAGGAGGGAA TTCCCCCAGA TCAGCAGCGT CTGATCTTCG CCGGCAAGCA GCTTGAGGAT 
GGACGCACCC TGTCCGATTA CAACATCCAG AAGGAGTCCA CCCTTCACTT GGTCCTTCGT 
CTCCGTGGTG GTATGCAGAT CTTCGTGAAG ACTTTGACCG GAAAGACCAT CACCCTCGAG 
GTAGAGCCCT CGGACACCAT TGAGAATGTT AAGGCTAAGA TCCAAGACAA GGAGGGAATT 
CCCCCAGATC AGCAGCGTCT GATCTTCGCC GGCAAGCAGC TTGAGGATGG ACGCACCCTG 
TCCGATTACA ACATCCAGAA GGAATCGACC CTTCACTTGG TGCTCCGTCT CCGTGGAGGA 
ATCCAGGCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ubi-p63E ( NM_079185.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523909.2 Drosophila melanogaster Ubiquitin-63E (Ubi-p63E), transcript variant B, mRNA.

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CloneID	OFa17429
Clone ID Related Accession (Same CDS sequence)	NM_168043.3 , NM_206272.1 , NM_079185.4 , NM_001274454.1
Accession Version	NM_079185.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ubiquitin-63E, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Feb 27, 2003 this sequence version replaced NM_079185.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGATCT TTGTGAAGAC TTTGACCGGA AAGACCATCA CCCTCGAGGT AGAGCCCTCG 
GACACCATTG AGAATGTTAA GGCTAAGATC CAAGACAAGG AAGGAATTCC CCCAGATCAG 
CAGCGTCTGA TCTTCGCCGG CAAGCAACTG GAAGACGGAC GCACCCTGTC CGATTACAAC 
ATTCAGAAGG AGTCCACCCT TCACTTGGTC CTTCGTCTCC GTGGTGGTAT GCAGATCTTC 
GTGAAGACTT TGACCGGAAA GACCATCACC CTCGAGGTAG AGCCATCGGA CACCATTGAG 
AATGTGAAGG CCAAGATTCA GGATAAGGAG GGAATCCCCC CAGATCAGCA GCGTCTGATC 
TTCGCTGGCA AGCAGCTTGA GGATGGACGC ACCCTGTCCG ATTACAACAT CCAGAAGGAG 
TCCACCCTTC ACTTGGTCCT TCGTCTCCGT GGTGGTATGC AGATCTTCGT GAAGACTTTG 
ACCGGAAAGA CCATCACCCT CGAGGTAGAG CCATCGGACA CAATTGAGAA TGTTAAGGCT 
AAGATCCAAG ACAAGGAGGG AATTCCCCCA GATCAGCAGC GTCTGATCTT CGCCGGCAAG 
CAGCTTGAGG ATGGACGCAC CCTGTCCGAT TACAACATCC AGAAGGAGTC CACCCTTCAC 
TTGGTCCTTC GTCTCCGTGG TGGTATGCAG ATCTTCGTGA AGACTTTGAC CGGAAAGACC 
ATCACCCTCG AGGTAGAGCC TTCGGACACC ATTGAGAATG TTAAGGCTAA GATCCAAGAC 
AAGGAGGGAA TTCCCCCAGA TCAGCAGCGT CTGATCTTCG CTGGCAAGCA GCTTGAGGAT 
GGACGCACCC TGTCCGATTA CAACATTCAG AAGGAGTCCA CCCTTCACTT GGTCCTTCGT 
CTTCGTGGTG GTATGCAGAT CTTCGTGAAG ACTTTGACCG GAAAGACCAT CACCCTCGAG 
GTAGAGCCAT CGGACACAAT TGAGAATGTT AAGGCTAAGA TCCAAGACAA GGAGGGAATT 
CCCCCAGATC AGCAGCGTCT GATCTTCGCC GGCAAGCAGC TTGAGGATGG ACGCACCCTG 
TCCGATTACA ACATCCAGAA GGAGTCCACC CTTCACTTGG TCCTTCGTCT CCGTGGTGGT 
ATGCAGATCT TCGTGAAGAC TTTGACCGGA AAGACCATCA CCCTCGAGGT AGAGCCATCG 
GACACCATTG AGAATGTGAA GGCCAAGATT CAGGATAAGG AGGGAATCCC CCCAGATCAG 
CAGCGTCTGA TCTTCGCCGG CAAGCAGCTT GAGGATGGAC GCACCCTGTC CGATTACAAC 
ATCCAGAAGG AGTCCACCCT TCACTTGGTC CTTCGTCTCC GTGGTGGTAT GCAGATCTTC 
GTGAAGACTT TGACCGGAAA GACCATCACC CTCGAGGTAG AGCCTTCGGA CACCATTGAG 
AATGTTAAGG CTAAGATCCA AGACAAGGAG GGAATTCCCC CAGATCAGCA GCGTCTGATC 
TTCGCCGGCA AGCAGCTTGA GGATGGACGC ACCCTGTCCG ATTACAACAT CCAGAAGGAG 
TCCACCCTTC ACTTGGTCCT TCGTCTTCGT GGTGGTATGC AGATCTTCGT GAAGACTTTG 
ACCGGAAAGA CCATCACCCT CGAGGTAGAG CCATCGGACA CAATTGAGAA TGTTAAGGCT 
AAGATCCAAG ACAAGGAGGG AATTCCCCCA GATCAGCAGC GTCTGATCTT CGCCGGCAAG 
CAGCTTGAGG ATGGACGCAC CCTGTCCGAT TACAACATCC AGAAGGAGTC CACCCTTCAC 
TTGGTCCTTC GTCTCCGTGG TGGTATGCAG ATCTTCGTGA AGACTTTGAC CGGAAAGACC 
ATCACCCTCG AGGTAGAGCC TTCGGACACC ATTGAGAATG TTAAGGCTAA GATCCAAGAC 
AAGGAGGGAA TTCCCCCAGA TCAGCAGCGT CTGATCTTCG CCGGCAAGCA GCTTGAGGAT 
GGACGCACCC TGTCCGATTA CAACATCCAG AAGGAGTCCA CCCTTCACTT GGTCCTTCGT 
CTCCGTGGTG GTATGCAGAT CTTCGTGAAG ACTTTGACCG GAAAGACCAT CACCCTCGAG 
GTAGAGCCCT CGGACACCAT TGAGAATGTT AAGGCTAAGA TCCAAGACAA GGAGGGAATT 
CCCCCAGATC AGCAGCGTCT GATCTTCGCC GGCAAGCAGC TTGAGGATGG ACGCACCCTG 
TCCGATTACA ACATCCAGAA GGAATCGACC CTTCACTTGG TGCTCCGTCT CCGTGGAGGA 
ATCCAGGCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523909.2
CDS	259..2550
Translation	MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGIQA

Target ORF information:

RefSeq Version	NM_079185.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ubiquitin-63E (Ubi-p63E), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079185.4

ORF Insert Sequence:

ATGCAGATCT TTGTGAAGAC TTTGACCGGA AAGACCATCA CCCTCGAGGT AGAGCCCTCG 
GACACCATTG AGAATGTTAA GGCTAAGATC CAAGACAAGG AAGGAATTCC CCCAGATCAG 
CAGCGTCTGA TCTTCGCCGG CAAGCAACTG GAAGACGGAC GCACCCTGTC CGATTACAAC 
ATTCAGAAGG AGTCCACCCT TCACTTGGTC CTTCGTCTCC GTGGTGGTAT GCAGATCTTC 
GTGAAGACTT TGACCGGAAA GACCATCACC CTCGAGGTAG AGCCATCGGA CACCATTGAG 
AATGTGAAGG CCAAGATTCA GGATAAGGAG GGAATCCCCC CAGATCAGCA GCGTCTGATC 
TTCGCTGGCA AGCAGCTTGA GGATGGACGC ACCCTGTCCG ATTACAACAT CCAGAAGGAG 
TCCACCCTTC ACTTGGTCCT TCGTCTCCGT GGTGGTATGC AGATCTTCGT GAAGACTTTG 
ACCGGAAAGA CCATCACCCT CGAGGTAGAG CCATCGGACA CAATTGAGAA TGTTAAGGCT 
AAGATCCAAG ACAAGGAGGG AATTCCCCCA GATCAGCAGC GTCTGATCTT CGCCGGCAAG 
CAGCTTGAGG ATGGACGCAC CCTGTCCGAT TACAACATCC AGAAGGAGTC CACCCTTCAC 
TTGGTCCTTC GTCTCCGTGG TGGTATGCAG ATCTTCGTGA AGACTTTGAC CGGAAAGACC 
ATCACCCTCG AGGTAGAGCC TTCGGACACC ATTGAGAATG TTAAGGCTAA GATCCAAGAC 
AAGGAGGGAA TTCCCCCAGA TCAGCAGCGT CTGATCTTCG CTGGCAAGCA GCTTGAGGAT 
GGACGCACCC TGTCCGATTA CAACATTCAG AAGGAGTCCA CCCTTCACTT GGTCCTTCGT 
CTTCGTGGTG GTATGCAGAT CTTCGTGAAG ACTTTGACCG GAAAGACCAT CACCCTCGAG 
GTAGAGCCAT CGGACACAAT TGAGAATGTT AAGGCTAAGA TCCAAGACAA GGAGGGAATT 
CCCCCAGATC AGCAGCGTCT GATCTTCGCC GGCAAGCAGC TTGAGGATGG ACGCACCCTG 
TCCGATTACA ACATCCAGAA GGAGTCCACC CTTCACTTGG TCCTTCGTCT CCGTGGTGGT 
ATGCAGATCT TCGTGAAGAC TTTGACCGGA AAGACCATCA CCCTCGAGGT AGAGCCATCG 
GACACCATTG AGAATGTGAA GGCCAAGATT CAGGATAAGG AGGGAATCCC CCCAGATCAG 
CAGCGTCTGA TCTTCGCCGG CAAGCAGCTT GAGGATGGAC GCACCCTGTC CGATTACAAC 
ATCCAGAAGG AGTCCACCCT TCACTTGGTC CTTCGTCTCC GTGGTGGTAT GCAGATCTTC 
GTGAAGACTT TGACCGGAAA GACCATCACC CTCGAGGTAG AGCCTTCGGA CACCATTGAG 
AATGTTAAGG CTAAGATCCA AGACAAGGAG GGAATTCCCC CAGATCAGCA GCGTCTGATC 
TTCGCCGGCA AGCAGCTTGA GGATGGACGC ACCCTGTCCG ATTACAACAT CCAGAAGGAG 
TCCACCCTTC ACTTGGTCCT TCGTCTTCGT GGTGGTATGC AGATCTTCGT GAAGACTTTG 
ACCGGAAAGA CCATCACCCT CGAGGTAGAG CCATCGGACA CAATTGAGAA TGTTAAGGCT 
AAGATCCAAG ACAAGGAGGG AATTCCCCCA GATCAGCAGC GTCTGATCTT CGCCGGCAAG 
CAGCTTGAGG ATGGACGCAC CCTGTCCGAT TACAACATCC AGAAGGAGTC CACCCTTCAC 
TTGGTCCTTC GTCTCCGTGG TGGTATGCAG ATCTTCGTGA AGACTTTGAC CGGAAAGACC 
ATCACCCTCG AGGTAGAGCC TTCGGACACC ATTGAGAATG TTAAGGCTAA GATCCAAGAC 
AAGGAGGGAA TTCCCCCAGA TCAGCAGCGT CTGATCTTCG CCGGCAAGCA GCTTGAGGAT 
GGACGCACCC TGTCCGATTA CAACATCCAG AAGGAGTCCA CCCTTCACTT GGTCCTTCGT 
CTCCGTGGTG GTATGCAGAT CTTCGTGAAG ACTTTGACCG GAAAGACCAT CACCCTCGAG 
GTAGAGCCCT CGGACACCAT TGAGAATGTT AAGGCTAAGA TCCAAGACAA GGAGGGAATT 
CCCCCAGATC AGCAGCGTCT GATCTTCGCC GGCAAGCAGC TTGAGGATGG ACGCACCCTG 
TCCGATTACA ACATCCAGAA GGAATCGACC CTTCACTTGG TGCTCCGTCT CCGTGGAGGA 
ATCCAGGCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ubi-p63E ( NM_001274454.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261383.1 Drosophila melanogaster Ubiquitin-63E (Ubi-p63E), transcript variant D, mRNA.

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CloneID	OFa17429
Clone ID Related Accession (Same CDS sequence)	NM_168043.3 , NM_206272.1 , NM_079185.4 , NM_001274454.1
Accession Version	NM_001274454.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ubiquitin-63E, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGATCT TTGTGAAGAC TTTGACCGGA AAGACCATCA CCCTCGAGGT AGAGCCCTCG 
GACACCATTG AGAATGTTAA GGCTAAGATC CAAGACAAGG AAGGAATTCC CCCAGATCAG 
CAGCGTCTGA TCTTCGCCGG CAAGCAACTG GAAGACGGAC GCACCCTGTC CGATTACAAC 
ATTCAGAAGG AGTCCACCCT TCACTTGGTC CTTCGTCTCC GTGGTGGTAT GCAGATCTTC 
GTGAAGACTT TGACCGGAAA GACCATCACC CTCGAGGTAG AGCCATCGGA CACCATTGAG 
AATGTGAAGG CCAAGATTCA GGATAAGGAG GGAATCCCCC CAGATCAGCA GCGTCTGATC 
TTCGCTGGCA AGCAGCTTGA GGATGGACGC ACCCTGTCCG ATTACAACAT CCAGAAGGAG 
TCCACCCTTC ACTTGGTCCT TCGTCTCCGT GGTGGTATGC AGATCTTCGT GAAGACTTTG 
ACCGGAAAGA CCATCACCCT CGAGGTAGAG CCATCGGACA CAATTGAGAA TGTTAAGGCT 
AAGATCCAAG ACAAGGAGGG AATTCCCCCA GATCAGCAGC GTCTGATCTT CGCCGGCAAG 
CAGCTTGAGG ATGGACGCAC CCTGTCCGAT TACAACATCC AGAAGGAGTC CACCCTTCAC 
TTGGTCCTTC GTCTCCGTGG TGGTATGCAG ATCTTCGTGA AGACTTTGAC CGGAAAGACC 
ATCACCCTCG AGGTAGAGCC TTCGGACACC ATTGAGAATG TTAAGGCTAA GATCCAAGAC 
AAGGAGGGAA TTCCCCCAGA TCAGCAGCGT CTGATCTTCG CTGGCAAGCA GCTTGAGGAT 
GGACGCACCC TGTCCGATTA CAACATTCAG AAGGAGTCCA CCCTTCACTT GGTCCTTCGT 
CTTCGTGGTG GTATGCAGAT CTTCGTGAAG ACTTTGACCG GAAAGACCAT CACCCTCGAG 
GTAGAGCCAT CGGACACAAT TGAGAATGTT AAGGCTAAGA TCCAAGACAA GGAGGGAATT 
CCCCCAGATC AGCAGCGTCT GATCTTCGCC GGCAAGCAGC TTGAGGATGG ACGCACCCTG 
TCCGATTACA ACATCCAGAA GGAGTCCACC CTTCACTTGG TCCTTCGTCT CCGTGGTGGT 
ATGCAGATCT TCGTGAAGAC TTTGACCGGA AAGACCATCA CCCTCGAGGT AGAGCCATCG 
GACACCATTG AGAATGTGAA GGCCAAGATT CAGGATAAGG AGGGAATCCC CCCAGATCAG 
CAGCGTCTGA TCTTCGCCGG CAAGCAGCTT GAGGATGGAC GCACCCTGTC CGATTACAAC 
ATCCAGAAGG AGTCCACCCT TCACTTGGTC CTTCGTCTCC GTGGTGGTAT GCAGATCTTC 
GTGAAGACTT TGACCGGAAA GACCATCACC CTCGAGGTAG AGCCTTCGGA CACCATTGAG 
AATGTTAAGG CTAAGATCCA AGACAAGGAG GGAATTCCCC CAGATCAGCA GCGTCTGATC 
TTCGCCGGCA AGCAGCTTGA GGATGGACGC ACCCTGTCCG ATTACAACAT CCAGAAGGAG 
TCCACCCTTC ACTTGGTCCT TCGTCTTCGT GGTGGTATGC AGATCTTCGT GAAGACTTTG 
ACCGGAAAGA CCATCACCCT CGAGGTAGAG CCATCGGACA CAATTGAGAA TGTTAAGGCT 
AAGATCCAAG ACAAGGAGGG AATTCCCCCA GATCAGCAGC GTCTGATCTT CGCCGGCAAG 
CAGCTTGAGG ATGGACGCAC CCTGTCCGAT TACAACATCC AGAAGGAGTC CACCCTTCAC 
TTGGTCCTTC GTCTCCGTGG TGGTATGCAG ATCTTCGTGA AGACTTTGAC CGGAAAGACC 
ATCACCCTCG AGGTAGAGCC TTCGGACACC ATTGAGAATG TTAAGGCTAA GATCCAAGAC 
AAGGAGGGAA TTCCCCCAGA TCAGCAGCGT CTGATCTTCG CCGGCAAGCA GCTTGAGGAT 
GGACGCACCC TGTCCGATTA CAACATCCAG AAGGAGTCCA CCCTTCACTT GGTCCTTCGT 
CTCCGTGGTG GTATGCAGAT CTTCGTGAAG ACTTTGACCG GAAAGACCAT CACCCTCGAG 
GTAGAGCCCT CGGACACCAT TGAGAATGTT AAGGCTAAGA TCCAAGACAA GGAGGGAATT 
CCCCCAGATC AGCAGCGTCT GATCTTCGCC GGCAAGCAGC TTGAGGATGG ACGCACCCTG 
TCCGATTACA ACATCCAGAA GGAATCGACC CTTCACTTGG TGCTCCGTCT CCGTGGAGGA 
ATCCAGGCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261383.1
CDS	124..2415
Translation	MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGIQA

Target ORF information:

RefSeq Version	NM_001274454.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ubiquitin-63E (Ubi-p63E), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274454.1

ORF Insert Sequence:

ATGCAGATCT TTGTGAAGAC TTTGACCGGA AAGACCATCA CCCTCGAGGT AGAGCCCTCG 
GACACCATTG AGAATGTTAA GGCTAAGATC CAAGACAAGG AAGGAATTCC CCCAGATCAG 
CAGCGTCTGA TCTTCGCCGG CAAGCAACTG GAAGACGGAC GCACCCTGTC CGATTACAAC 
ATTCAGAAGG AGTCCACCCT TCACTTGGTC CTTCGTCTCC GTGGTGGTAT GCAGATCTTC 
GTGAAGACTT TGACCGGAAA GACCATCACC CTCGAGGTAG AGCCATCGGA CACCATTGAG 
AATGTGAAGG CCAAGATTCA GGATAAGGAG GGAATCCCCC CAGATCAGCA GCGTCTGATC 
TTCGCTGGCA AGCAGCTTGA GGATGGACGC ACCCTGTCCG ATTACAACAT CCAGAAGGAG 
TCCACCCTTC ACTTGGTCCT TCGTCTCCGT GGTGGTATGC AGATCTTCGT GAAGACTTTG 
ACCGGAAAGA CCATCACCCT CGAGGTAGAG CCATCGGACA CAATTGAGAA TGTTAAGGCT 
AAGATCCAAG ACAAGGAGGG AATTCCCCCA GATCAGCAGC GTCTGATCTT CGCCGGCAAG 
CAGCTTGAGG ATGGACGCAC CCTGTCCGAT TACAACATCC AGAAGGAGTC CACCCTTCAC 
TTGGTCCTTC GTCTCCGTGG TGGTATGCAG ATCTTCGTGA AGACTTTGAC CGGAAAGACC 
ATCACCCTCG AGGTAGAGCC TTCGGACACC ATTGAGAATG TTAAGGCTAA GATCCAAGAC 
AAGGAGGGAA TTCCCCCAGA TCAGCAGCGT CTGATCTTCG CTGGCAAGCA GCTTGAGGAT 
GGACGCACCC TGTCCGATTA CAACATTCAG AAGGAGTCCA CCCTTCACTT GGTCCTTCGT 
CTTCGTGGTG GTATGCAGAT CTTCGTGAAG ACTTTGACCG GAAAGACCAT CACCCTCGAG 
GTAGAGCCAT CGGACACAAT TGAGAATGTT AAGGCTAAGA TCCAAGACAA GGAGGGAATT 
CCCCCAGATC AGCAGCGTCT GATCTTCGCC GGCAAGCAGC TTGAGGATGG ACGCACCCTG 
TCCGATTACA ACATCCAGAA GGAGTCCACC CTTCACTTGG TCCTTCGTCT CCGTGGTGGT 
ATGCAGATCT TCGTGAAGAC TTTGACCGGA AAGACCATCA CCCTCGAGGT AGAGCCATCG 
GACACCATTG AGAATGTGAA GGCCAAGATT CAGGATAAGG AGGGAATCCC CCCAGATCAG 
CAGCGTCTGA TCTTCGCCGG CAAGCAGCTT GAGGATGGAC GCACCCTGTC CGATTACAAC 
ATCCAGAAGG AGTCCACCCT TCACTTGGTC CTTCGTCTCC GTGGTGGTAT GCAGATCTTC 
GTGAAGACTT TGACCGGAAA GACCATCACC CTCGAGGTAG AGCCTTCGGA CACCATTGAG 
AATGTTAAGG CTAAGATCCA AGACAAGGAG GGAATTCCCC CAGATCAGCA GCGTCTGATC 
TTCGCCGGCA AGCAGCTTGA GGATGGACGC ACCCTGTCCG ATTACAACAT CCAGAAGGAG 
TCCACCCTTC ACTTGGTCCT TCGTCTTCGT GGTGGTATGC AGATCTTCGT GAAGACTTTG 
ACCGGAAAGA CCATCACCCT CGAGGTAGAG CCATCGGACA CAATTGAGAA TGTTAAGGCT 
AAGATCCAAG ACAAGGAGGG AATTCCCCCA GATCAGCAGC GTCTGATCTT CGCCGGCAAG 
CAGCTTGAGG ATGGACGCAC CCTGTCCGAT TACAACATCC AGAAGGAGTC CACCCTTCAC 
TTGGTCCTTC GTCTCCGTGG TGGTATGCAG ATCTTCGTGA AGACTTTGAC CGGAAAGACC 
ATCACCCTCG AGGTAGAGCC TTCGGACACC ATTGAGAATG TTAAGGCTAA GATCCAAGAC 
AAGGAGGGAA TTCCCCCAGA TCAGCAGCGT CTGATCTTCG CCGGCAAGCA GCTTGAGGAT 
GGACGCACCC TGTCCGATTA CAACATCCAG AAGGAGTCCA CCCTTCACTT GGTCCTTCGT 
CTCCGTGGTG GTATGCAGAT CTTCGTGAAG ACTTTGACCG GAAAGACCAT CACCCTCGAG 
GTAGAGCCCT CGGACACCAT TGAGAATGTT AAGGCTAAGA TCCAAGACAA GGAGGGAATT 
CCCCCAGATC AGCAGCGTCT GATCTTCGCC GGCAAGCAGC TTGAGGATGG ACGCACCCTG 
TCCGATTACA ACATCCAGAA GGAATCGACC CTTCACTTGG TGCTCCGTCT CCGTGGAGGA 
ATCCAGGCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ubi-p63E cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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