Scsalpha1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Scsalpha1
Entrez Gene ID	38447
Full Name	Succinyl coenzyme A synthetase alpha subunit
Synonyms	CG1065,Dmel\CG1065,Scs-alpha,scsalpha
General protein information	Preferred Names Succinyl coenzyme A synthetase alpha subunit Names CG1065 gene product from transcript CG1065-RA CG1065-PA CG1065-PB Scsalpha-PA Scsalpha-PB succinyl coenzyme A synthatase, alpha subunit succinyl coenzyme A synthetase alpha subunit succinyl-CoA synthetase alpha-subunit
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 63F1-63F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001202115.2	NP_001189044.1	Succinyl-coenzyme A synthetase alpha subunit 1, isoform B
NM_079181.4	NP_523905.2	Succinyl-coenzyme A synthetase alpha subunit 1, isoform A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SUCLG1	XP_001164971.1
Danio rerio (zebrafish)	suclg1	NP_001002577.2
Drosophila melanogaster (fruit fly)	Scsalpha	NP_523905.2
Saccharomyces cerevisiae (baker's yeast)	LSC1	NP_014785.3
	SUCLG1	XP_001082705.2
Canis lupus familiaris (dog)	SUCLG1	XP_532985.3
Rattus norvegicus (Norway rat)	Suclg1	NP_446204.2
Gallus gallus (chicken)	SUCLG1	NP_001012910.1
Schizosaccharomyces pombe (fission yeast)	SPAC16E8.17c	NP_594230.1
Magnaporthe oryzae (rice blast fungus)	MGG_05480	XP_003710345.1
Xenopus tropicalis (tropical clawed frog)	suclg1	NP_001025589.1
Caenorhabditis elegans (roundworm)	sucl-1	NP_510450.1
Eremothecium gossypii	AGOS_AFR134C	NP_985681.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001312	XP_321835.2
Caenorhabditis elegans (roundworm)	sucl-2	NP_497288.1
Arabidopsis thaliana (thale cress)	AT5G23250	NP_197716.1
Arabidopsis thaliana (thale cress)	AT5G08300	NP_196447.1
Homo sapiens (human)	SUCLG1	NP_003840.2
Bos taurus (cattle)	SUCLG1	NP_001030254.1
Mus musculus (house mouse)	Suclg1	NP_063932.2
Kluyveromyces lactis	KLLA0E03235g	XP_454091.1
Neurospora crassa	NCU01227	XP_961593.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Drosophila SCS deficiency, designated Scsalpha(KO), contained a high level of succinyl-CoA, a substrate for the enzyme, and altered levels of various metabolites in TCA cycle and glycolysis, indicating that the energy metabolism was impaired
Title: Deficiency of succinyl-CoA synthetase ? subunit delays development, impairs locomotor activity and reduces survival under starvation in Drosophila.

The following Scsalpha1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Scsalpha1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17421	NM_079181.4 Latest version!	Drosophila melanogaster Succinyl-coenzyme A synthetase alpha subunit 1 (Scsalpha1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa17421	NM_001202115.2 Latest version!	Drosophila melanogaster Succinyl-coenzyme A synthetase alpha subunit 1 (Scsalpha1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Scsalpha1 ( NM_001202115.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189044.1 Drosophila melanogaster Succinyl-coenzyme A synthetase alpha subunit 1 (Scsalpha1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa17421
Clone ID Related Accession (Same CDS sequence)	NM_001202115.2 , NM_079181.4
Accession Version	NM_001202115.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Succinyl-coenzyme A synthetase alpha subunit 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001202115.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGCTT CAATGAGGGC GCTGCTGAAA GTGCGAGATG GCTTCGTGGC CGGAGTGCGC 
TGCAATTCGC AGTACAACAA GACGCGCGGC AACTTGAAGC TGAACGGCGA TTCGCGGGTG 
ATCTGCCAGG GATTCACCGG CAAACAGGGC ACGTTCCACA GCCAACAGGC TCTGGAGTAC 
GGCACCAAGT TGGTCGGCGG CATTTCCCCC AAGAAGGGAG GCACCCAGCA TCTGGGACTG 
CCCGTCTTCG CTTCGGTGGC GGAGGCCAAG AAGGCCACCG ATCCACATGC CACCGTCATC 
TATGTGCCAC CACCGGGCGC TGCGGCCGCC ATCATCGAGG CTCTGGAAGC GGAGATCCCC 
CTGATCGTTT GCATTACGGA GGGTGTGCCG CAGCACGACA TGGTGAAGGT GAAGCACGCC 
CTCATCAGCC AGAGCAAGTC GCGATTGGTG GGTCCCAACT GCCCGGGAAT CATCGCCCCC 
GAACAGTGCA AGATCGGCAT CATGCCGGGT CACATTCACA AGCGCGGCAA GATCGGCGTG 
GTCTCGCGCT CGGGAACCTT GACCTACGAG GCCGTGCACC AGACCACGGA GGTGGGTCTG 
GGCCAGACCC TGTGCGTGGG CATTGGAGGC GATCCGTTCA ACGGAACCGA CTTCATCGAC 
TGCCTGGAGG TCTTCCTCAA GGATCCCGAG ACCAAGGGCA TCATCCTGAT CGGCGAGATC 
GGAGGCGTTG CCGAGGAGAA GGCTGCCGAC TACCTGACCG AGTACAATTC GGGCATCAAG 
GCCAAGCCTG TCGTCTCGTT CATTGCCGGA GTGTCGGCGC CACCCGGCCG TCGCATGGGT 
CACGCTGGAG CCATCATTTC CGGAGGAAAG GGAGGTGCCA ACGACAAGAT CGCCGCCCTG 
GAGAAGGCCG GCGTCATTGT GACCAGGAGT CCTGCCAAAA TGGGCCACGA GCTCTTCAAG 
GAGATGAAGC GTCTGGAGTT AGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189044.1
CDS	109..1095
Translation	MAASMRALLKVRDGFVAGVRCNSQYNKTRGNLKLNGDSRVICQGFTGKQGTFHSQQALEYGTKLVGGISPKKGGTQHLGLPVFASVAEAKKATDPHATVIYVPPPGAAAAIIEALEAEIPLIVCITEGVPQHDMVKVKHALISQSKSRLVGPNCPGIIAPEQCKIGIMPGHIHKRGKIGVVSRSGTLTYEAVHQTTEVGLGQTLCVGIGGDPFNGTDFIDCLEVFLKDPETKGIILIGEIGGVAEEKAADYLTEYNSGIKAKPVVSFIAGVSAPPGRRMGHAGAIISGGKGGANDKIAALEKAGVIVTRSPAKMGHELFKEMKRLELV

Target ORF information:

RefSeq Version	NM_001202115.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Succinyl-coenzyme A synthetase alpha subunit 1 (Scsalpha1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202115.2

ORF Insert Sequence:

ATGGCTGCTT CAATGAGGGC GCTGCTGAAA GTGCGAGATG GCTTCGTGGC CGGAGTGCGC 
TGCAATTCGC AGTACAACAA GACGCGCGGC AACTTGAAGC TGAACGGCGA TTCGCGGGTG 
ATCTGCCAGG GATTCACCGG CAAACAGGGC ACGTTCCACA GCCAACAGGC TCTGGAGTAC 
GGCACCAAGT TGGTCGGCGG CATTTCCCCC AAGAAGGGAG GCACCCAGCA TCTGGGACTG 
CCCGTCTTCG CTTCGGTGGC GGAGGCCAAG AAGGCCACCG ATCCACATGC CACCGTCATC 
TATGTGCCAC CACCGGGCGC TGCGGCCGCC ATCATCGAGG CTCTGGAAGC GGAGATCCCC 
CTGATCGTTT GCATTACGGA GGGTGTGCCG CAGCACGACA TGGTGAAGGT GAAGCACGCC 
CTCATCAGCC AGAGCAAGTC GCGATTGGTG GGTCCCAACT GCCCGGGAAT CATCGCCCCC 
GAACAGTGCA AGATCGGCAT CATGCCGGGT CACATTCACA AGCGCGGCAA GATCGGCGTG 
GTCTCGCGCT CGGGAACCTT GACCTACGAG GCCGTGCACC AGACCACGGA GGTGGGTCTG 
GGCCAGACCC TGTGCGTGGG CATTGGAGGC GATCCGTTCA ACGGAACCGA CTTCATCGAC 
TGCCTGGAGG TCTTCCTCAA GGATCCCGAG ACCAAGGGCA TCATCCTGAT CGGCGAGATC 
GGAGGCGTTG CCGAGGAGAA GGCTGCCGAC TACCTGACCG AGTACAATTC GGGCATCAAG 
GCCAAGCCTG TCGTCTCGTT CATTGCCGGA GTGTCGGCGC CACCCGGCCG TCGCATGGGT 
CACGCTGGAG CCATCATTTC CGGAGGAAAG GGAGGTGCCA ACGACAAGAT CGCCGCCCTG 
GAGAAGGCCG GCGTCATTGT GACCAGGAGT CCTGCCAAAA TGGGCCACGA GCTCTTCAAG 
GAGATGAAGC GTCTGGAGTT AGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scsalpha1 ( NM_079181.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523905.2 Drosophila melanogaster Succinyl-coenzyme A synthetase alpha subunit 1 (Scsalpha1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa17421
Clone ID Related Accession (Same CDS sequence)	NM_001202115.2 , NM_079181.4
Accession Version	NM_079181.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Succinyl-coenzyme A synthetase alpha subunit 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079181.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGCTT CAATGAGGGC GCTGCTGAAA GTGCGAGATG GCTTCGTGGC CGGAGTGCGC 
TGCAATTCGC AGTACAACAA GACGCGCGGC AACTTGAAGC TGAACGGCGA TTCGCGGGTG 
ATCTGCCAGG GATTCACCGG CAAACAGGGC ACGTTCCACA GCCAACAGGC TCTGGAGTAC 
GGCACCAAGT TGGTCGGCGG CATTTCCCCC AAGAAGGGAG GCACCCAGCA TCTGGGACTG 
CCCGTCTTCG CTTCGGTGGC GGAGGCCAAG AAGGCCACCG ATCCACATGC CACCGTCATC 
TATGTGCCAC CACCGGGCGC TGCGGCCGCC ATCATCGAGG CTCTGGAAGC GGAGATCCCC 
CTGATCGTTT GCATTACGGA GGGTGTGCCG CAGCACGACA TGGTGAAGGT GAAGCACGCC 
CTCATCAGCC AGAGCAAGTC GCGATTGGTG GGTCCCAACT GCCCGGGAAT CATCGCCCCC 
GAACAGTGCA AGATCGGCAT CATGCCGGGT CACATTCACA AGCGCGGCAA GATCGGCGTG 
GTCTCGCGCT CGGGAACCTT GACCTACGAG GCCGTGCACC AGACCACGGA GGTGGGTCTG 
GGCCAGACCC TGTGCGTGGG CATTGGAGGC GATCCGTTCA ACGGAACCGA CTTCATCGAC 
TGCCTGGAGG TCTTCCTCAA GGATCCCGAG ACCAAGGGCA TCATCCTGAT CGGCGAGATC 
GGAGGCGTTG CCGAGGAGAA GGCTGCCGAC TACCTGACCG AGTACAATTC GGGCATCAAG 
GCCAAGCCTG TCGTCTCGTT CATTGCCGGA GTGTCGGCGC CACCCGGCCG TCGCATGGGT 
CACGCTGGAG CCATCATTTC CGGAGGAAAG GGAGGTGCCA ACGACAAGAT CGCCGCCCTG 
GAGAAGGCCG GCGTCATTGT GACCAGGAGT CCTGCCAAAA TGGGCCACGA GCTCTTCAAG 
GAGATGAAGC GTCTGGAGTT AGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523905.2
CDS	109..1095
Translation	MAASMRALLKVRDGFVAGVRCNSQYNKTRGNLKLNGDSRVICQGFTGKQGTFHSQQALEYGTKLVGGISPKKGGTQHLGLPVFASVAEAKKATDPHATVIYVPPPGAAAAIIEALEAEIPLIVCITEGVPQHDMVKVKHALISQSKSRLVGPNCPGIIAPEQCKIGIMPGHIHKRGKIGVVSRSGTLTYEAVHQTTEVGLGQTLCVGIGGDPFNGTDFIDCLEVFLKDPETKGIILIGEIGGVAEEKAADYLTEYNSGIKAKPVVSFIAGVSAPPGRRMGHAGAIISGGKGGANDKIAALEKAGVIVTRSPAKMGHELFKEMKRLELV

Target ORF information:

RefSeq Version	NM_079181.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Succinyl-coenzyme A synthetase alpha subunit 1 (Scsalpha1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079181.4

ORF Insert Sequence:

ATGGCTGCTT CAATGAGGGC GCTGCTGAAA GTGCGAGATG GCTTCGTGGC CGGAGTGCGC 
TGCAATTCGC AGTACAACAA GACGCGCGGC AACTTGAAGC TGAACGGCGA TTCGCGGGTG 
ATCTGCCAGG GATTCACCGG CAAACAGGGC ACGTTCCACA GCCAACAGGC TCTGGAGTAC 
GGCACCAAGT TGGTCGGCGG CATTTCCCCC AAGAAGGGAG GCACCCAGCA TCTGGGACTG 
CCCGTCTTCG CTTCGGTGGC GGAGGCCAAG AAGGCCACCG ATCCACATGC CACCGTCATC 
TATGTGCCAC CACCGGGCGC TGCGGCCGCC ATCATCGAGG CTCTGGAAGC GGAGATCCCC 
CTGATCGTTT GCATTACGGA GGGTGTGCCG CAGCACGACA TGGTGAAGGT GAAGCACGCC 
CTCATCAGCC AGAGCAAGTC GCGATTGGTG GGTCCCAACT GCCCGGGAAT CATCGCCCCC 
GAACAGTGCA AGATCGGCAT CATGCCGGGT CACATTCACA AGCGCGGCAA GATCGGCGTG 
GTCTCGCGCT CGGGAACCTT GACCTACGAG GCCGTGCACC AGACCACGGA GGTGGGTCTG 
GGCCAGACCC TGTGCGTGGG CATTGGAGGC GATCCGTTCA ACGGAACCGA CTTCATCGAC 
TGCCTGGAGG TCTTCCTCAA GGATCCCGAG ACCAAGGGCA TCATCCTGAT CGGCGAGATC 
GGAGGCGTTG CCGAGGAGAA GGCTGCCGAC TACCTGACCG AGTACAATTC GGGCATCAAG 
GCCAAGCCTG TCGTCTCGTT CATTGCCGGA GTGTCGGCGC CACCCGGCCG TCGCATGGGT 
CACGCTGGAG CCATCATTTC CGGAGGAAAG GGAGGTGCCA ACGACAAGAT CGCCGCCCTG 
GAGAAGGCCG GCGTCATTGT GACCAGGAGT CCTGCCAAAA TGGGCCACGA GCTCTTCAAG 
GAGATGAAGC GTCTGGAGTT AGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Scsalpha1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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