Atg2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atg2
Entrez Gene ID	38344
Full Name	Autophagy-related 2
Synonyms	ATG2,CG1241,Dmel\CG1241,EP3697,atg2
General protein information	Preferred Names Autophagy-related 2 Names CG1241 gene product from transcript CG1241-RB Atg2-PA Atg2-PB Autophagy-specific gene 2 CG1241-PA CG1241-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 62F3-62F4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274412.1	NP_001261341.1	Autophagy-related 2, isoform B
NM_139491.2	NP_647748.1	Autophagy-related 2, isoform A

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Atg2b	NP_083930.5
Canis lupus familiaris (dog)	ATG2B	XP_537549.3
Gallus gallus (chicken)	ATG2B	XP_421350.3
Drosophila melanogaster (fruit fly)	Atg2	NP_001261341.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004092	XP_312964.5
Xenopus tropicalis (tropical clawed frog)	atg2b	NP_001072626.1
Homo sapiens (human)	ATG2B	NP_060506.5
Bos taurus (cattle)	ATG2B	NP_001193682.1
Pan troglodytes (chimpanzee)	ATG2B	XP_510152.3
	ATG2B	XP_001101467.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

double knockdown of tlk and atg2 by RNAi did not have a synergistic effect. These results suggested that ATG2 may function downstream of TLK. In addition to a role in development, tlk and atg2 RNAi could rescue calcium overload-induced cell death
Title: Tousled-like kinase mediated a new type of cell death pathway in Drosophila.

Atg2 and ArfGAP1 may have roles in modifying the eye pigmentation phenotype of AP-3 mutants in Drosophila melanogaster
Title: Identification of Atg2 and ArfGAP1 as Candidate Genetic Modifiers of the Eye Pigmentation Phenotype of Adaptor Protein-3 (AP-3) Mutants in Drosophila melanogaster.

The transmembrane protein Atg9 accumulates on refractory to Sigma P (Ref(2)P) aggregates in Atg7, Atg8a and Atg2 mutants.
Title: Different effects of Atg2 and Atg18 mutations on Atg8a and Atg9 trafficking during starvation in Drosophila.

The following Atg2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atg2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17327	NM_139491.2 Latest version!	Drosophila melanogaster Autophagy-related 2 (Atg2), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa17327	NM_001274412.1 Latest version!	Drosophila melanogaster Autophagy-related 2 (Atg2), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atg2 ( NM_001274412.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261341.1 Drosophila melanogaster Autophagy-related 2 (Atg2), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa17327
Clone ID Related Accession (Same CDS sequence)	NM_001274412.1 , NM_139491.2
Accession Version	NM_001274412.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Autophagy-related 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTGGT TCAATCCCTG GGATGGCCTC AAAACGAAAA TGTGTCGCTA CCTGCTGCAG 
CGATACCTGG GCCAGTTTTT CGAGAATAAC TTGAACCTGG AGCAGCTGAA GGTGGACCTT 
TACAATGGCA AGGCTGTGGT GGAGGACATT TTCCTAAAGG TCAACGCCTT CAACGATCTC 
TTCGAGGACC AGGGCTGGGC CTTCGAGGTG GTCTCGGGCC ACATTGGCCG CCTGACTGTC 
GTGGTGCCGT GGAATGCGCT GATGACCAAC GACAGTAGCC TGGAGATCCA CAATCTAACC 
ATCACACTGA GGCCAGTCAC ACGCTACCAG AGCGGCACCA CCATGCTGGA GTCCATGTGG 
TCGTCGGTGA GCAGCTCGAT GCAAATGGCC GAGGAGTGCA TGAAGCAGGT GGATGACGAT 
GTGCCCTTCC TGAACCACAA TAACGCCCTG ATTGGGTTGG AGAAGTTCGC AGAGACCATC 
GACAACGTGC TCAACCGCAT ACGCGCCAAG CTGACAAATA CTACTTTGAA CATTGAGTAC 
ATACTGCCCA GATCGGACCG GAAGCTGGTG CTCTCCTTCC AGGCCAGCCA TGTGGAGTAT 
AAGAACAAGA CGGGCTACGA GATGCCCATG TCCACCTCCC AGGACACTGA AACCGAAGAA 
GATCCCAATA GCAGCTTCAA TGCACTGCCC ATGATAGCCA AGCACAATTT GGTGATCGAG 
GGACTTAGTT TGCACACCTC CGAAGTGCTT GATGTGATGG ATCACCAGTT TGGACCCACT 
CCCTACGAGC AGCTCTGCAA GATTGTGGAG CTCAAGGGCG CGCAAAACAT CCAGATCAAC 
ATCAAGCAGA CGGAAAATAT AGTGGGGCCC AAGGTCAGCC TAGAACTGAG TCTGGACGAC 
ATATACTTCA TGCTAACGCC GCGTCAAATT CACCTGCTCA TCGAGTTCTC AAAGGGCTTC 
AACTGCGGCG GTCAGCAGGA GCGACCCAAA AAGGGCAGAA GCTTGAAATC GGCTCCGCCC 
AGTGAGTATC AGATGCACAG TTTGCAGCAG CCCACCCGGA TGACGGGGAT TCTGGGCCAG 
AATGCAGACT GGTGTACAGG AATGTCAGAG GCGGATCCCT CCGAATATTC CGTTTACGGA 
AATCCCCCGA GGAGCGGTTA CGCACGGAGT CGAAAGATGA GCGAATCCAC AAACAGCATG 
GTCACCTCCT GTACCAATAC TCTGCAGCAG GAGTTGGGCT ACACAGAGAA GTCCGGCGAG 
ATCCTCAAGT TCAAGGTGCA GATCTCGAAA ATCTACGGAA TTGCTTTGCA CCAGGACATT 
CTTATCCAGA ACACGGCCAA TATCGACAGT TGCAGCACGG TTTTCGATCA CGCAAGTTAC 
CTTCGCTACT CGGACACTGC CAGTGGGTTC TTCTTGGGCA CGCTCCTGGA GAATCACATC 
CCGCTGCAAA ATCAGCAGTA CCTTCTGCTA CGAGCAGCCC CAATAATCGT TAGTGGAAGC 
CAGCAGCGCT ATTTCCAGGA GCTCACATCG AGAACTACTC TCTCCGCCAC TGCGGTGGAT 
CTCATCGAGG TCCTGGACAA TTCCAAGGAG CATCTGCTTC AGTTCGACCG CCAGAGAAAC 
TGCCCGGATG GCTACCACAT TCGACCGGAG ATCGTGCTCA CCCAGAGCTC ATCGTTCATG 
TTCAAGCAGA ATCACAATCG TTGCTCTAAC AAAATTGAAT GCAGTTTGGA TGAATGTACG 
GCGGAATTGG ATATATCTAT TTACGATCGA CTAGGGGCGC TCTTTGGGAG CTCTCCGTTT 
TCCGGGGATT CGGCGTCGTC AACGCCCTAT CCAGATGATC CCAACCAAAC GGAGTTTGTG 
GTGAAGTCCG AAAATCTCCG TCTTCACTTG CGTTTCCCTG TGGTGGATGG CAGAGCCGCC 
AACGATCCGC TAAAGATTCC CTGGTGGAAG AAGAATGTGC GACGTGATTT CTTGGCAGTG 
GAGTTCCGCT CCCTGGTGGT TAGTTCGAGG TCTCAGATAA GCATCGTTTC GGATGAACTA 
GATGTGTTCT ATTGTGACGC GGATAAGCAG AGTGCCGTGC ATCTCCTGAA GTGCCAGCAA 
AAAATGCAGC CGAACAAGAA GATCCAAATT GACGTACTGC AGCTAAAGGA CACCTCGGAT 
GGCCAAAAGA AGGCAAGGAA GTCTCCGTTT AGCTCAAAAT GTACCTTTGG CTACACCTCC 
CATGTGGAGG GACTTGATGG TAGCGTGGAC AAAACCGATT CCCTTTTACC CGGCGAAACG 
GAGGAGATCA ATGAATTCTG CGACAGTTGC ACACAGTCCT CCAAGCTGAA GATATTTGCG 
TTTATACCGC ACGTAAAAGT TGTGCTGGAA TCCAAGAGCA TGTACGAACT GATATACAAC 
CGCTTGAACG GAGATCTCTT TATGTGGGAA CCACGTTCTC CGCACTATGA CATCAGCCCG 
GATAACGAAG TCCCAGAGTC AAAAGACCTG TACGCAGAGC CACAGCTGGA AGAGTTTAGA 
CGAAACCTGT TGCTCAGTAC GAGCGCCATG GAAAGTAGTA TTTACTTTTC CATGGCGGAG 
CCAACGGCTT CTGCTCCACC GGCGCCACCG GTGCGTAATG AAGCCTTCTC CTTTGAGTTG 
TTTGTAGAGC AGGGAACTTT GATTCTGTTC TCGCATCTTC TGGATCCCGA GACCAACCAG 
CGATCCAAGG AGTGCGGTAA GTTTCAGGTG AACTTAAAGG AGCTGCGTTT GTTTACAGTG 
AATGGCTTGG ACAACGATTC GACTCGGAGT TTCTTTTCCA TCCAACTGGG CGAGATCGAT 
GCCTTGCATT GCGGGAATAC GCTGCAGGAT TTGGAGCTGG CTTGGAGTGA TTTGGCGGAT 
GAAAAGCTGC TGCCCACCAT CTATAACTTT GCAAGTGTTC ATCAGCAGCA GGACAGGAAA 
AGAATGGAGG TTTTATCCCT CGTGGCTGAG ATCAAGAAGC AACCGGAGCA GCGCATCAAG 
AGAATGAAGA TGTCCTTCGG GATATGTGGC GCCATTCTGC AGCATCACTC CTGCCACTCG 
GAGCATTCTT GGCTGAATCA ACTGATCGAC TTTATGGACG TGGCCGACTT TCCCATCGAG 
GAATACGAAC CGTTTGCTCT GGTCTCCGAG CTGCAGTTGC ATGTGTGGAA TGCTGGGATC 
GACTATCGCC CGAAATTCTT TCCACAACGT GCGTTCCTGG ATCTGGGCTA CTGTACCTTG 
AGCAGCAATA TCATATCATC GATGAGTGGC TGCACCTTGC GATTGCTGGC CGAGGATTGT 
GTCCTGCAAC TGGGATTGGT TAAGGAGGAG TCCAACGACT CCCTGATACC TGTGCTGAAT 
CTGGGACTCT TGAATATATC CTTCCGTTTG AATGCAGAAG GTAGTGGACA ACCGCGAGTG 
GATTTGCGCT CTTCCATCCA CGATATGCAT CTGAAGACTT GCTACGATTC GGCAGCAGCT 
TTGGCCCAAC TTATAGCCTA TGTGGCCAAC GACAGGGACT TGTGTCCGCC AGAGGAGCCC 
ATCTTGAATG AGGACACTAA GCCTGCAGAG CCGAAACCCA ACGAGGAGCA TGAGATGAGC 
GATCACACCC AGAACCATGT GAGCAAATTG ATGGCCGATG CTGTGATAGA CGTAGAGTCC 
TCGCCCAATC TCAGGGCAAT GAAATCTCCA GGGGGTCGGG AGGAGGGCAT TGAGATATTC 
TACTTCCCGG ATGAGCCCAA AGAGAAACGG AAATCCACGT CCGCCAAGCT GCAGACCAAG 
TCACTGATGG TCGAGAGTCC CTCGGTCATC GGAGACATCC TGGACTTTGA ATCGAATGTG 
ATCTTGCAGT CCTACTACCA CCAGAGCCAA GTGCAACCGC AGACATCACT GGGCTCCCAG 
GTGCAGCAGG AGCTGGGTTC CCTGGGCAAC GCGTCCCTGG AGGAGCGGGA TTTCGACATC 
ATACACGACG AGGAGATTTC ACGCATGGAC AAGTTCGGTG TCAAGCAAAT TTACATCTCC 
GAGGACCCTT TGCAGATTGT GGACAATCAC TTTGAGCTGC CGCATGAGAA AGTGGACCTG 
CTTAGGCCTC CATCTAATTT CCCAGTGGCC GAGAGCACTT ACACCCTTTG CGAGATGACA 
TTCACATGGC ACCTTTATGG CGGCCGGGAT TTCCCCGATG AACCACTCAA GAAATCCTCT 
TCGTCGGCAA CCAGTGGCTT TGGCATGTCC GATACCTACA AGTATGGCGT GTCTCAAGCT 
CAAGAGCAGG ATAAACAGGA CAAAAAGGGT TCCAAGAAGG GTCTGCTCAA ACAACCCAAA 
GGATCGCAGC GCAATCTGGA GGTTCTAGTG GAGATGCAGC TCTCCAAGAT TCGATTCTCC 
TACGAAACCT ATCCGTTGAC CTCCATGTAC TCGTCCCGTC AGGTGCTCCT TGTGTCGGAA 
ATTGAAATAA GAGATAGGTT GCGCTCCTCG GACATTAACA AGTTTCTGTA CCATCCGACG 
GGCAACAATT TATCCCATAA GCCGGACGAG AACATGGTCA TAGTGAAGGC TCTGAATGTG 
CGTCCGAATC CGCAAAAGAG CTCCGCGGAA GAGTGCTCCC TGAGGGTCTC CATTTTGCCC 
ATAAAACTCA ACATTGACCA GGATACTCTG CTCTTTCTCG AGGACTTCTT TTCGGGCATG 
TTCCGAAACT CCGCTAGTGC TGCTGGTGGC ACCAAAACGG AGGGCAGCAG TTCGTCGGCC 
ACGGATATGC CGGTGATGTC GGTGAGCAAA ATGCCGGAGG AGTTATCCGA TAAATTGCCC 
GATTCGGAAG TTAAGGAGAT GGTGGAGAGG AACCTGAATG TGCTGATTGA AGAGAACAGC 
TTGGATGTGG AGTTGGAGGA GGATCCTGCC ACGGCTCCAC CTGTGTTCTT CCGCGAGGTG 
ATCTTCAGTC CAGCACTACC CATTTGCTTT GACTATCATG GACGGCGGAT TGAACTCTCC 
CGCGGACCGG TCACCGGCCT AATCATGGGT CTTGCCCAGC TGCAGGGATC CGGAATTAAT 
TTGAGGGAAA TTGTGAACCG CAGAGGCATT CTGGGATGGA ACAAACTGTG CGAGTTCCTG 
GCCAAGGAGT GGCTGAAGGA CATCAAGCGG AATCAATTGC CCAATATCCT AAGCGGGATT 
GGACCCACGA ATGCGGTGCT GCAGCTTTTC CAGGGTATCT ACGATCTTTT CCGGCTGCCC 
ATCGAGCAGT ACAACAAGGA TGGACGAATT ATCAGGGGAT TCCAGTTGGG TGCCCAGAGT 
TTCACCGCTC GCACAGCGCT GGCTGCCTTG GAGATCACCT CCAGGATTAT ACACCTGCTG 
CAATTCACGG CGGAGACTAC CTTCGATATG CTATCTGCAG GTCCTTCCAT GAAGAAGCGG 
AAGGGCGGCA GGCAGGGCAA ACGGAGGCGA CAGGGACGAC CCAAGGACCT GCGCGAGGGC 
GTGGCCAACG CCTATACCAT AGTGAGAGAG GGCATCAACG AATCGGCCAA CACACTCATC 
GAAGCCGCCA TCACGGAGCA CGACCAGAAG GGCTACAGCG GAGCGGTGGG CGCGGTGGTA 
CGCCAGATCC CTCAGCTGGT CGTCTGCCCG GCGGTGCTGG CCACCCAGGC CACCACCAAC 
ATCCTGGGCG GCGCCAAGAG CTCGCTGATT CCGGAGGCCA AACTGGAGGC GCGCGACAAG 
TGGAAGCAGG AGATCCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261341.1
CDS	282..6002
Translation	MSWFNPWDGLKTKMCRYLLQRYLGQFFENNLNLEQLKVDLYNGKAVVEDIFLKVNAFNDLFEDQGWAFEVVSGHIGRLTVVVPWNALMTNDSSLEIHNLTITLRPVTRYQSGTTMLESMWSSVSSSMQMAEECMKQVDDDVPFLNHNNALIGLEKFAETIDNVLNRIRAKLTNTTLNIEYILPRSDRKLVLSFQASHVEYKNKTGYEMPMSTSQDTETEEDPNSSFNALPMIAKHNLVIEGLSLHTSEVLDVMDHQFGPTPYEQLCKIVELKGAQNIQINIKQTENIVGPKVSLELSLDDIYFMLTPRQIHLLIEFSKGFNCGGQQERPKKGRSLKSAPPSEYQMHSLQQPTRMTGILGQNADWCTGMSEADPSEYSVYGNPPRSGYARSRKMSESTNSMVTSCTNTLQQELGYTEKSGEILKFKVQISKIYGIALHQDILIQNTANIDSCSTVFDHASYLRYSDTASGFFLGTLLENHIPLQNQQYLLLRAAPIIVSGSQQRYFQELTSRTTLSATAVDLIEVLDNSKEHLLQFDRQRNCPDGYHIRPEIVLTQSSSFMFKQNHNRCSNKIECSLDECTAELDISIYDRLGALFGSSPFSGDSASSTPYPDDPNQTEFVVKSENLRLHLRFPVVDGRAANDPLKIPWWKKNVRRDFLAVEFRSLVVSSRSQISIVSDELDVFYCDADKQSAVHLLKCQQKMQPNKKIQIDVLQLKDTSDGQKKARKSPFSSKCTFGYTSHVEGLDGSVDKTDSLLPGETEEINEFCDSCTQSSKLKIFAFIPHVKVVLESKSMYELIYNRLNGDLFMWEPRSPHYDISPDNEVPESKDLYAEPQLEEFRRNLLLSTSAMESSIYFSMAEPTASAPPAPPVRNEAFSFELFVEQGTLILFSHLLDPETNQRSKECGKFQVNLKELRLFTVNGLDNDSTRSFFSIQLGEIDALHCGNTLQDLELAWSDLADEKLLPTIYNFASVHQQQDRKRMEVLSLVAEIKKQPEQRIKRMKMSFGICGAILQHHSCHSEHSWLNQLIDFMDVADFPIEEYEPFALVSELQLHVWNAGIDYRPKFFPQRAFLDLGYCTLSSNIISSMSGCTLRLLAEDCVLQLGLVKEESNDSLIPVLNLGLLNISFRLNAEGSGQPRVDLRSSIHDMHLKTCYDSAAALAQLIAYVANDRDLCPPEEPILNEDTKPAEPKPNEEHEMSDHTQNHVSKLMADAVIDVESSPNLRAMKSPGGREEGIEIFYFPDEPKEKRKSTSAKLQTKSLMVESPSVIGDILDFESNVILQSYYHQSQVQPQTSLGSQVQQELGSLGNASLEERDFDIIHDEEISRMDKFGVKQIYISEDPLQIVDNHFELPHEKVDLLRPPSNFPVAESTYTLCEMTFTWHLYGGRDFPDEPLKKSSSSATSGFGMSDTYKYGVSQAQEQDKQDKKGSKKGLLKQPKGSQRNLEVLVEMQLSKIRFSYETYPLTSMYSSRQVLLVSEIEIRDRLRSSDINKFLYHPTGNNLSHKPDENMVIVKALNVRPNPQKSSAEECSLRVSILPIKLNIDQDTLLFLEDFFSGMFRNSASAAGGTKTEGSSSSATDMPVMSVSKMPEELSDKLPDSEVKEMVERNLNVLIEENSLDVELEEDPATAPPVFFREVIFSPALPICFDYHGRRIELSRGPVTGLIMGLAQLQGSGINLREIVNRRGILGWNKLCEFLAKEWLKDIKRNQLPNILSGIGPTNAVLQLFQGIYDLFRLPIEQYNKDGRIIRGFQLGAQSFTARTALAALEITSRIIHLLQFTAETTFDMLSAGPSMKKRKGGRQGKRRRQGRPKDLREGVANAYTIVREGINESANTLIEAAITEHDQKGYSGAVGAVVRQIPQLVVCPAVLATQATTNILGGAKSSLIPEAKLEARDKWKQEIH

Target ORF information:

RefSeq Version	NM_001274412.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Autophagy-related 2 (Atg2), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274412.1

ORF Insert Sequence:

ATGTCGTGGT TCAATCCCTG GGATGGCCTC AAAACGAAAA TGTGTCGCTA CCTGCTGCAG 
CGATACCTGG GCCAGTTTTT CGAGAATAAC TTGAACCTGG AGCAGCTGAA GGTGGACCTT 
TACAATGGCA AGGCTGTGGT GGAGGACATT TTCCTAAAGG TCAACGCCTT CAACGATCTC 
TTCGAGGACC AGGGCTGGGC CTTCGAGGTG GTCTCGGGCC ACATTGGCCG CCTGACTGTC 
GTGGTGCCGT GGAATGCGCT GATGACCAAC GACAGTAGCC TGGAGATCCA CAATCTAACC 
ATCACACTGA GGCCAGTCAC ACGCTACCAG AGCGGCACCA CCATGCTGGA GTCCATGTGG 
TCGTCGGTGA GCAGCTCGAT GCAAATGGCC GAGGAGTGCA TGAAGCAGGT GGATGACGAT 
GTGCCCTTCC TGAACCACAA TAACGCCCTG ATTGGGTTGG AGAAGTTCGC AGAGACCATC 
GACAACGTGC TCAACCGCAT ACGCGCCAAG CTGACAAATA CTACTTTGAA CATTGAGTAC 
ATACTGCCCA GATCGGACCG GAAGCTGGTG CTCTCCTTCC AGGCCAGCCA TGTGGAGTAT 
AAGAACAAGA CGGGCTACGA GATGCCCATG TCCACCTCCC AGGACACTGA AACCGAAGAA 
GATCCCAATA GCAGCTTCAA TGCACTGCCC ATGATAGCCA AGCACAATTT GGTGATCGAG 
GGACTTAGTT TGCACACCTC CGAAGTGCTT GATGTGATGG ATCACCAGTT TGGACCCACT 
CCCTACGAGC AGCTCTGCAA GATTGTGGAG CTCAAGGGCG CGCAAAACAT CCAGATCAAC 
ATCAAGCAGA CGGAAAATAT AGTGGGGCCC AAGGTCAGCC TAGAACTGAG TCTGGACGAC 
ATATACTTCA TGCTAACGCC GCGTCAAATT CACCTGCTCA TCGAGTTCTC AAAGGGCTTC 
AACTGCGGCG GTCAGCAGGA GCGACCCAAA AAGGGCAGAA GCTTGAAATC GGCTCCGCCC 
AGTGAGTATC AGATGCACAG TTTGCAGCAG CCCACCCGGA TGACGGGGAT TCTGGGCCAG 
AATGCAGACT GGTGTACAGG AATGTCAGAG GCGGATCCCT CCGAATATTC CGTTTACGGA 
AATCCCCCGA GGAGCGGTTA CGCACGGAGT CGAAAGATGA GCGAATCCAC AAACAGCATG 
GTCACCTCCT GTACCAATAC TCTGCAGCAG GAGTTGGGCT ACACAGAGAA GTCCGGCGAG 
ATCCTCAAGT TCAAGGTGCA GATCTCGAAA ATCTACGGAA TTGCTTTGCA CCAGGACATT 
CTTATCCAGA ACACGGCCAA TATCGACAGT TGCAGCACGG TTTTCGATCA CGCAAGTTAC 
CTTCGCTACT CGGACACTGC CAGTGGGTTC TTCTTGGGCA CGCTCCTGGA GAATCACATC 
CCGCTGCAAA ATCAGCAGTA CCTTCTGCTA CGAGCAGCCC CAATAATCGT TAGTGGAAGC 
CAGCAGCGCT ATTTCCAGGA GCTCACATCG AGAACTACTC TCTCCGCCAC TGCGGTGGAT 
CTCATCGAGG TCCTGGACAA TTCCAAGGAG CATCTGCTTC AGTTCGACCG CCAGAGAAAC 
TGCCCGGATG GCTACCACAT TCGACCGGAG ATCGTGCTCA CCCAGAGCTC ATCGTTCATG 
TTCAAGCAGA ATCACAATCG TTGCTCTAAC AAAATTGAAT GCAGTTTGGA TGAATGTACG 
GCGGAATTGG ATATATCTAT TTACGATCGA CTAGGGGCGC TCTTTGGGAG CTCTCCGTTT 
TCCGGGGATT CGGCGTCGTC AACGCCCTAT CCAGATGATC CCAACCAAAC GGAGTTTGTG 
GTGAAGTCCG AAAATCTCCG TCTTCACTTG CGTTTCCCTG TGGTGGATGG CAGAGCCGCC 
AACGATCCGC TAAAGATTCC CTGGTGGAAG AAGAATGTGC GACGTGATTT CTTGGCAGTG 
GAGTTCCGCT CCCTGGTGGT TAGTTCGAGG TCTCAGATAA GCATCGTTTC GGATGAACTA 
GATGTGTTCT ATTGTGACGC GGATAAGCAG AGTGCCGTGC ATCTCCTGAA GTGCCAGCAA 
AAAATGCAGC CGAACAAGAA GATCCAAATT GACGTACTGC AGCTAAAGGA CACCTCGGAT 
GGCCAAAAGA AGGCAAGGAA GTCTCCGTTT AGCTCAAAAT GTACCTTTGG CTACACCTCC 
CATGTGGAGG GACTTGATGG TAGCGTGGAC AAAACCGATT CCCTTTTACC CGGCGAAACG 
GAGGAGATCA ATGAATTCTG CGACAGTTGC ACACAGTCCT CCAAGCTGAA GATATTTGCG 
TTTATACCGC ACGTAAAAGT TGTGCTGGAA TCCAAGAGCA TGTACGAACT GATATACAAC 
CGCTTGAACG GAGATCTCTT TATGTGGGAA CCACGTTCTC CGCACTATGA CATCAGCCCG 
GATAACGAAG TCCCAGAGTC AAAAGACCTG TACGCAGAGC CACAGCTGGA AGAGTTTAGA 
CGAAACCTGT TGCTCAGTAC GAGCGCCATG GAAAGTAGTA TTTACTTTTC CATGGCGGAG 
CCAACGGCTT CTGCTCCACC GGCGCCACCG GTGCGTAATG AAGCCTTCTC CTTTGAGTTG 
TTTGTAGAGC AGGGAACTTT GATTCTGTTC TCGCATCTTC TGGATCCCGA GACCAACCAG 
CGATCCAAGG AGTGCGGTAA GTTTCAGGTG AACTTAAAGG AGCTGCGTTT GTTTACAGTG 
AATGGCTTGG ACAACGATTC GACTCGGAGT TTCTTTTCCA TCCAACTGGG CGAGATCGAT 
GCCTTGCATT GCGGGAATAC GCTGCAGGAT TTGGAGCTGG CTTGGAGTGA TTTGGCGGAT 
GAAAAGCTGC TGCCCACCAT CTATAACTTT GCAAGTGTTC ATCAGCAGCA GGACAGGAAA 
AGAATGGAGG TTTTATCCCT CGTGGCTGAG ATCAAGAAGC AACCGGAGCA GCGCATCAAG 
AGAATGAAGA TGTCCTTCGG GATATGTGGC GCCATTCTGC AGCATCACTC CTGCCACTCG 
GAGCATTCTT GGCTGAATCA ACTGATCGAC TTTATGGACG TGGCCGACTT TCCCATCGAG 
GAATACGAAC CGTTTGCTCT GGTCTCCGAG CTGCAGTTGC ATGTGTGGAA TGCTGGGATC 
GACTATCGCC CGAAATTCTT TCCACAACGT GCGTTCCTGG ATCTGGGCTA CTGTACCTTG 
AGCAGCAATA TCATATCATC GATGAGTGGC TGCACCTTGC GATTGCTGGC CGAGGATTGT 
GTCCTGCAAC TGGGATTGGT TAAGGAGGAG TCCAACGACT CCCTGATACC TGTGCTGAAT 
CTGGGACTCT TGAATATATC CTTCCGTTTG AATGCAGAAG GTAGTGGACA ACCGCGAGTG 
GATTTGCGCT CTTCCATCCA CGATATGCAT CTGAAGACTT GCTACGATTC GGCAGCAGCT 
TTGGCCCAAC TTATAGCCTA TGTGGCCAAC GACAGGGACT TGTGTCCGCC AGAGGAGCCC 
ATCTTGAATG AGGACACTAA GCCTGCAGAG CCGAAACCCA ACGAGGAGCA TGAGATGAGC 
GATCACACCC AGAACCATGT GAGCAAATTG ATGGCCGATG CTGTGATAGA CGTAGAGTCC 
TCGCCCAATC TCAGGGCAAT GAAATCTCCA GGGGGTCGGG AGGAGGGCAT TGAGATATTC 
TACTTCCCGG ATGAGCCCAA AGAGAAACGG AAATCCACGT CCGCCAAGCT GCAGACCAAG 
TCACTGATGG TCGAGAGTCC CTCGGTCATC GGAGACATCC TGGACTTTGA ATCGAATGTG 
ATCTTGCAGT CCTACTACCA CCAGAGCCAA GTGCAACCGC AGACATCACT GGGCTCCCAG 
GTGCAGCAGG AGCTGGGTTC CCTGGGCAAC GCGTCCCTGG AGGAGCGGGA TTTCGACATC 
ATACACGACG AGGAGATTTC ACGCATGGAC AAGTTCGGTG TCAAGCAAAT TTACATCTCC 
GAGGACCCTT TGCAGATTGT GGACAATCAC TTTGAGCTGC CGCATGAGAA AGTGGACCTG 
CTTAGGCCTC CATCTAATTT CCCAGTGGCC GAGAGCACTT ACACCCTTTG CGAGATGACA 
TTCACATGGC ACCTTTATGG CGGCCGGGAT TTCCCCGATG AACCACTCAA GAAATCCTCT 
TCGTCGGCAA CCAGTGGCTT TGGCATGTCC GATACCTACA AGTATGGCGT GTCTCAAGCT 
CAAGAGCAGG ATAAACAGGA CAAAAAGGGT TCCAAGAAGG GTCTGCTCAA ACAACCCAAA 
GGATCGCAGC GCAATCTGGA GGTTCTAGTG GAGATGCAGC TCTCCAAGAT TCGATTCTCC 
TACGAAACCT ATCCGTTGAC CTCCATGTAC TCGTCCCGTC AGGTGCTCCT TGTGTCGGAA 
ATTGAAATAA GAGATAGGTT GCGCTCCTCG GACATTAACA AGTTTCTGTA CCATCCGACG 
GGCAACAATT TATCCCATAA GCCGGACGAG AACATGGTCA TAGTGAAGGC TCTGAATGTG 
CGTCCGAATC CGCAAAAGAG CTCCGCGGAA GAGTGCTCCC TGAGGGTCTC CATTTTGCCC 
ATAAAACTCA ACATTGACCA GGATACTCTG CTCTTTCTCG AGGACTTCTT TTCGGGCATG 
TTCCGAAACT CCGCTAGTGC TGCTGGTGGC ACCAAAACGG AGGGCAGCAG TTCGTCGGCC 
ACGGATATGC CGGTGATGTC GGTGAGCAAA ATGCCGGAGG AGTTATCCGA TAAATTGCCC 
GATTCGGAAG TTAAGGAGAT GGTGGAGAGG AACCTGAATG TGCTGATTGA AGAGAACAGC 
TTGGATGTGG AGTTGGAGGA GGATCCTGCC ACGGCTCCAC CTGTGTTCTT CCGCGAGGTG 
ATCTTCAGTC CAGCACTACC CATTTGCTTT GACTATCATG GACGGCGGAT TGAACTCTCC 
CGCGGACCGG TCACCGGCCT AATCATGGGT CTTGCCCAGC TGCAGGGATC CGGAATTAAT 
TTGAGGGAAA TTGTGAACCG CAGAGGCATT CTGGGATGGA ACAAACTGTG CGAGTTCCTG 
GCCAAGGAGT GGCTGAAGGA CATCAAGCGG AATCAATTGC CCAATATCCT AAGCGGGATT 
GGACCCACGA ATGCGGTGCT GCAGCTTTTC CAGGGTATCT ACGATCTTTT CCGGCTGCCC 
ATCGAGCAGT ACAACAAGGA TGGACGAATT ATCAGGGGAT TCCAGTTGGG TGCCCAGAGT 
TTCACCGCTC GCACAGCGCT GGCTGCCTTG GAGATCACCT CCAGGATTAT ACACCTGCTG 
CAATTCACGG CGGAGACTAC CTTCGATATG CTATCTGCAG GTCCTTCCAT GAAGAAGCGG 
AAGGGCGGCA GGCAGGGCAA ACGGAGGCGA CAGGGACGAC CCAAGGACCT GCGCGAGGGC 
GTGGCCAACG CCTATACCAT AGTGAGAGAG GGCATCAACG AATCGGCCAA CACACTCATC 
GAAGCCGCCA TCACGGAGCA CGACCAGAAG GGCTACAGCG GAGCGGTGGG CGCGGTGGTA 
CGCCAGATCC CTCAGCTGGT CGTCTGCCCG GCGGTGCTGG CCACCCAGGC CACCACCAAC 
ATCCTGGGCG GCGCCAAGAG CTCGCTGATT CCGGAGGCCA AACTGGAGGC GCGCGACAAG 
TGGAAGCAGG AGATCCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atg2 ( NM_139491.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_647748.1 Drosophila melanogaster Autophagy-related 2 (Atg2), transcript variant A, mRNA.

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CloneID	OFa17327
Clone ID Related Accession (Same CDS sequence)	NM_001274412.1 , NM_139491.2
Accession Version	NM_139491.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Autophagy-related 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_139491.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTGGT TCAATCCCTG GGATGGCCTC AAAACGAAAA TGTGTCGCTA CCTGCTGCAG 
CGATACCTGG GCCAGTTTTT CGAGAATAAC TTGAACCTGG AGCAGCTGAA GGTGGACCTT 
TACAATGGCA AGGCTGTGGT GGAGGACATT TTCCTAAAGG TCAACGCCTT CAACGATCTC 
TTCGAGGACC AGGGCTGGGC CTTCGAGGTG GTCTCGGGCC ACATTGGCCG CCTGACTGTC 
GTGGTGCCGT GGAATGCGCT GATGACCAAC GACAGTAGCC TGGAGATCCA CAATCTAACC 
ATCACACTGA GGCCAGTCAC ACGCTACCAG AGCGGCACCA CCATGCTGGA GTCCATGTGG 
TCGTCGGTGA GCAGCTCGAT GCAAATGGCC GAGGAGTGCA TGAAGCAGGT GGATGACGAT 
GTGCCCTTCC TGAACCACAA TAACGCCCTG ATTGGGTTGG AGAAGTTCGC AGAGACCATC 
GACAACGTGC TCAACCGCAT ACGCGCCAAG CTGACAAATA CTACTTTGAA CATTGAGTAC 
ATACTGCCCA GATCGGACCG GAAGCTGGTG CTCTCCTTCC AGGCCAGCCA TGTGGAGTAT 
AAGAACAAGA CGGGCTACGA GATGCCCATG TCCACCTCCC AGGACACTGA AACCGAAGAA 
GATCCCAATA GCAGCTTCAA TGCACTGCCC ATGATAGCCA AGCACAATTT GGTGATCGAG 
GGACTTAGTT TGCACACCTC CGAAGTGCTT GATGTGATGG ATCACCAGTT TGGACCCACT 
CCCTACGAGC AGCTCTGCAA GATTGTGGAG CTCAAGGGCG CGCAAAACAT CCAGATCAAC 
ATCAAGCAGA CGGAAAATAT AGTGGGGCCC AAGGTCAGCC TAGAACTGAG TCTGGACGAC 
ATATACTTCA TGCTAACGCC GCGTCAAATT CACCTGCTCA TCGAGTTCTC AAAGGGCTTC 
AACTGCGGCG GTCAGCAGGA GCGACCCAAA AAGGGCAGAA GCTTGAAATC GGCTCCGCCC 
AGTGAGTATC AGATGCACAG TTTGCAGCAG CCCACCCGGA TGACGGGGAT TCTGGGCCAG 
AATGCAGACT GGTGTACAGG AATGTCAGAG GCGGATCCCT CCGAATATTC CGTTTACGGA 
AATCCCCCGA GGAGCGGTTA CGCACGGAGT CGAAAGATGA GCGAATCCAC AAACAGCATG 
GTCACCTCCT GTACCAATAC TCTGCAGCAG GAGTTGGGCT ACACAGAGAA GTCCGGCGAG 
ATCCTCAAGT TCAAGGTGCA GATCTCGAAA ATCTACGGAA TTGCTTTGCA CCAGGACATT 
CTTATCCAGA ACACGGCCAA TATCGACAGT TGCAGCACGG TTTTCGATCA CGCAAGTTAC 
CTTCGCTACT CGGACACTGC CAGTGGGTTC TTCTTGGGCA CGCTCCTGGA GAATCACATC 
CCGCTGCAAA ATCAGCAGTA CCTTCTGCTA CGAGCAGCCC CAATAATCGT TAGTGGAAGC 
CAGCAGCGCT ATTTCCAGGA GCTCACATCG AGAACTACTC TCTCCGCCAC TGCGGTGGAT 
CTCATCGAGG TCCTGGACAA TTCCAAGGAG CATCTGCTTC AGTTCGACCG CCAGAGAAAC 
TGCCCGGATG GCTACCACAT TCGACCGGAG ATCGTGCTCA CCCAGAGCTC ATCGTTCATG 
TTCAAGCAGA ATCACAATCG TTGCTCTAAC AAAATTGAAT GCAGTTTGGA TGAATGTACG 
GCGGAATTGG ATATATCTAT TTACGATCGA CTAGGGGCGC TCTTTGGGAG CTCTCCGTTT 
TCCGGGGATT CGGCGTCGTC AACGCCCTAT CCAGATGATC CCAACCAAAC GGAGTTTGTG 
GTGAAGTCCG AAAATCTCCG TCTTCACTTG CGTTTCCCTG TGGTGGATGG CAGAGCCGCC 
AACGATCCGC TAAAGATTCC CTGGTGGAAG AAGAATGTGC GACGTGATTT CTTGGCAGTG 
GAGTTCCGCT CCCTGGTGGT TAGTTCGAGG TCTCAGATAA GCATCGTTTC GGATGAACTA 
GATGTGTTCT ATTGTGACGC GGATAAGCAG AGTGCCGTGC ATCTCCTGAA GTGCCAGCAA 
AAAATGCAGC CGAACAAGAA GATCCAAATT GACGTACTGC AGCTAAAGGA CACCTCGGAT 
GGCCAAAAGA AGGCAAGGAA GTCTCCGTTT AGCTCAAAAT GTACCTTTGG CTACACCTCC 
CATGTGGAGG GACTTGATGG TAGCGTGGAC AAAACCGATT CCCTTTTACC CGGCGAAACG 
GAGGAGATCA ATGAATTCTG CGACAGTTGC ACACAGTCCT CCAAGCTGAA GATATTTGCG 
TTTATACCGC ACGTAAAAGT TGTGCTGGAA TCCAAGAGCA TGTACGAACT GATATACAAC 
CGCTTGAACG GAGATCTCTT TATGTGGGAA CCACGTTCTC CGCACTATGA CATCAGCCCG 
GATAACGAAG TCCCAGAGTC AAAAGACCTG TACGCAGAGC CACAGCTGGA AGAGTTTAGA 
CGAAACCTGT TGCTCAGTAC GAGCGCCATG GAAAGTAGTA TTTACTTTTC CATGGCGGAG 
CCAACGGCTT CTGCTCCACC GGCGCCACCG GTGCGTAATG AAGCCTTCTC CTTTGAGTTG 
TTTGTAGAGC AGGGAACTTT GATTCTGTTC TCGCATCTTC TGGATCCCGA GACCAACCAG 
CGATCCAAGG AGTGCGGTAA GTTTCAGGTG AACTTAAAGG AGCTGCGTTT GTTTACAGTG 
AATGGCTTGG ACAACGATTC GACTCGGAGT TTCTTTTCCA TCCAACTGGG CGAGATCGAT 
GCCTTGCATT GCGGGAATAC GCTGCAGGAT TTGGAGCTGG CTTGGAGTGA TTTGGCGGAT 
GAAAAGCTGC TGCCCACCAT CTATAACTTT GCAAGTGTTC ATCAGCAGCA GGACAGGAAA 
AGAATGGAGG TTTTATCCCT CGTGGCTGAG ATCAAGAAGC AACCGGAGCA GCGCATCAAG 
AGAATGAAGA TGTCCTTCGG GATATGTGGC GCCATTCTGC AGCATCACTC CTGCCACTCG 
GAGCATTCTT GGCTGAATCA ACTGATCGAC TTTATGGACG TGGCCGACTT TCCCATCGAG 
GAATACGAAC CGTTTGCTCT GGTCTCCGAG CTGCAGTTGC ATGTGTGGAA TGCTGGGATC 
GACTATCGCC CGAAATTCTT TCCACAACGT GCGTTCCTGG ATCTGGGCTA CTGTACCTTG 
AGCAGCAATA TCATATCATC GATGAGTGGC TGCACCTTGC GATTGCTGGC CGAGGATTGT 
GTCCTGCAAC TGGGATTGGT TAAGGAGGAG TCCAACGACT CCCTGATACC TGTGCTGAAT 
CTGGGACTCT TGAATATATC CTTCCGTTTG AATGCAGAAG GTAGTGGACA ACCGCGAGTG 
GATTTGCGCT CTTCCATCCA CGATATGCAT CTGAAGACTT GCTACGATTC GGCAGCAGCT 
TTGGCCCAAC TTATAGCCTA TGTGGCCAAC GACAGGGACT TGTGTCCGCC AGAGGAGCCC 
ATCTTGAATG AGGACACTAA GCCTGCAGAG CCGAAACCCA ACGAGGAGCA TGAGATGAGC 
GATCACACCC AGAACCATGT GAGCAAATTG ATGGCCGATG CTGTGATAGA CGTAGAGTCC 
TCGCCCAATC TCAGGGCAAT GAAATCTCCA GGGGGTCGGG AGGAGGGCAT TGAGATATTC 
TACTTCCCGG ATGAGCCCAA AGAGAAACGG AAATCCACGT CCGCCAAGCT GCAGACCAAG 
TCACTGATGG TCGAGAGTCC CTCGGTCATC GGAGACATCC TGGACTTTGA ATCGAATGTG 
ATCTTGCAGT CCTACTACCA CCAGAGCCAA GTGCAACCGC AGACATCACT GGGCTCCCAG 
GTGCAGCAGG AGCTGGGTTC CCTGGGCAAC GCGTCCCTGG AGGAGCGGGA TTTCGACATC 
ATACACGACG AGGAGATTTC ACGCATGGAC AAGTTCGGTG TCAAGCAAAT TTACATCTCC 
GAGGACCCTT TGCAGATTGT GGACAATCAC TTTGAGCTGC CGCATGAGAA AGTGGACCTG 
CTTAGGCCTC CATCTAATTT CCCAGTGGCC GAGAGCACTT ACACCCTTTG CGAGATGACA 
TTCACATGGC ACCTTTATGG CGGCCGGGAT TTCCCCGATG AACCACTCAA GAAATCCTCT 
TCGTCGGCAA CCAGTGGCTT TGGCATGTCC GATACCTACA AGTATGGCGT GTCTCAAGCT 
CAAGAGCAGG ATAAACAGGA CAAAAAGGGT TCCAAGAAGG GTCTGCTCAA ACAACCCAAA 
GGATCGCAGC GCAATCTGGA GGTTCTAGTG GAGATGCAGC TCTCCAAGAT TCGATTCTCC 
TACGAAACCT ATCCGTTGAC CTCCATGTAC TCGTCCCGTC AGGTGCTCCT TGTGTCGGAA 
ATTGAAATAA GAGATAGGTT GCGCTCCTCG GACATTAACA AGTTTCTGTA CCATCCGACG 
GGCAACAATT TATCCCATAA GCCGGACGAG AACATGGTCA TAGTGAAGGC TCTGAATGTG 
CGTCCGAATC CGCAAAAGAG CTCCGCGGAA GAGTGCTCCC TGAGGGTCTC CATTTTGCCC 
ATAAAACTCA ACATTGACCA GGATACTCTG CTCTTTCTCG AGGACTTCTT TTCGGGCATG 
TTCCGAAACT CCGCTAGTGC TGCTGGTGGC ACCAAAACGG AGGGCAGCAG TTCGTCGGCC 
ACGGATATGC CGGTGATGTC GGTGAGCAAA ATGCCGGAGG AGTTATCCGA TAAATTGCCC 
GATTCGGAAG TTAAGGAGAT GGTGGAGAGG AACCTGAATG TGCTGATTGA AGAGAACAGC 
TTGGATGTGG AGTTGGAGGA GGATCCTGCC ACGGCTCCAC CTGTGTTCTT CCGCGAGGTG 
ATCTTCAGTC CAGCACTACC CATTTGCTTT GACTATCATG GACGGCGGAT TGAACTCTCC 
CGCGGACCGG TCACCGGCCT AATCATGGGT CTTGCCCAGC TGCAGGGATC CGGAATTAAT 
TTGAGGGAAA TTGTGAACCG CAGAGGCATT CTGGGATGGA ACAAACTGTG CGAGTTCCTG 
GCCAAGGAGT GGCTGAAGGA CATCAAGCGG AATCAATTGC CCAATATCCT AAGCGGGATT 
GGACCCACGA ATGCGGTGCT GCAGCTTTTC CAGGGTATCT ACGATCTTTT CCGGCTGCCC 
ATCGAGCAGT ACAACAAGGA TGGACGAATT ATCAGGGGAT TCCAGTTGGG TGCCCAGAGT 
TTCACCGCTC GCACAGCGCT GGCTGCCTTG GAGATCACCT CCAGGATTAT ACACCTGCTG 
CAATTCACGG CGGAGACTAC CTTCGATATG CTATCTGCAG GTCCTTCCAT GAAGAAGCGG 
AAGGGCGGCA GGCAGGGCAA ACGGAGGCGA CAGGGACGAC CCAAGGACCT GCGCGAGGGC 
GTGGCCAACG CCTATACCAT AGTGAGAGAG GGCATCAACG AATCGGCCAA CACACTCATC 
GAAGCCGCCA TCACGGAGCA CGACCAGAAG GGCTACAGCG GAGCGGTGGG CGCGGTGGTA 
CGCCAGATCC CTCAGCTGGT CGTCTGCCCG GCGGTGCTGG CCACCCAGGC CACCACCAAC 
ATCCTGGGCG GCGCCAAGAG CTCGCTGATT CCGGAGGCCA AACTGGAGGC GCGCGACAAG 
TGGAAGCAGG AGATCCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_647748.1
CDS	275..5995
Translation	MSWFNPWDGLKTKMCRYLLQRYLGQFFENNLNLEQLKVDLYNGKAVVEDIFLKVNAFNDLFEDQGWAFEVVSGHIGRLTVVVPWNALMTNDSSLEIHNLTITLRPVTRYQSGTTMLESMWSSVSSSMQMAEECMKQVDDDVPFLNHNNALIGLEKFAETIDNVLNRIRAKLTNTTLNIEYILPRSDRKLVLSFQASHVEYKNKTGYEMPMSTSQDTETEEDPNSSFNALPMIAKHNLVIEGLSLHTSEVLDVMDHQFGPTPYEQLCKIVELKGAQNIQINIKQTENIVGPKVSLELSLDDIYFMLTPRQIHLLIEFSKGFNCGGQQERPKKGRSLKSAPPSEYQMHSLQQPTRMTGILGQNADWCTGMSEADPSEYSVYGNPPRSGYARSRKMSESTNSMVTSCTNTLQQELGYTEKSGEILKFKVQISKIYGIALHQDILIQNTANIDSCSTVFDHASYLRYSDTASGFFLGTLLENHIPLQNQQYLLLRAAPIIVSGSQQRYFQELTSRTTLSATAVDLIEVLDNSKEHLLQFDRQRNCPDGYHIRPEIVLTQSSSFMFKQNHNRCSNKIECSLDECTAELDISIYDRLGALFGSSPFSGDSASSTPYPDDPNQTEFVVKSENLRLHLRFPVVDGRAANDPLKIPWWKKNVRRDFLAVEFRSLVVSSRSQISIVSDELDVFYCDADKQSAVHLLKCQQKMQPNKKIQIDVLQLKDTSDGQKKARKSPFSSKCTFGYTSHVEGLDGSVDKTDSLLPGETEEINEFCDSCTQSSKLKIFAFIPHVKVVLESKSMYELIYNRLNGDLFMWEPRSPHYDISPDNEVPESKDLYAEPQLEEFRRNLLLSTSAMESSIYFSMAEPTASAPPAPPVRNEAFSFELFVEQGTLILFSHLLDPETNQRSKECGKFQVNLKELRLFTVNGLDNDSTRSFFSIQLGEIDALHCGNTLQDLELAWSDLADEKLLPTIYNFASVHQQQDRKRMEVLSLVAEIKKQPEQRIKRMKMSFGICGAILQHHSCHSEHSWLNQLIDFMDVADFPIEEYEPFALVSELQLHVWNAGIDYRPKFFPQRAFLDLGYCTLSSNIISSMSGCTLRLLAEDCVLQLGLVKEESNDSLIPVLNLGLLNISFRLNAEGSGQPRVDLRSSIHDMHLKTCYDSAAALAQLIAYVANDRDLCPPEEPILNEDTKPAEPKPNEEHEMSDHTQNHVSKLMADAVIDVESSPNLRAMKSPGGREEGIEIFYFPDEPKEKRKSTSAKLQTKSLMVESPSVIGDILDFESNVILQSYYHQSQVQPQTSLGSQVQQELGSLGNASLEERDFDIIHDEEISRMDKFGVKQIYISEDPLQIVDNHFELPHEKVDLLRPPSNFPVAESTYTLCEMTFTWHLYGGRDFPDEPLKKSSSSATSGFGMSDTYKYGVSQAQEQDKQDKKGSKKGLLKQPKGSQRNLEVLVEMQLSKIRFSYETYPLTSMYSSRQVLLVSEIEIRDRLRSSDINKFLYHPTGNNLSHKPDENMVIVKALNVRPNPQKSSAEECSLRVSILPIKLNIDQDTLLFLEDFFSGMFRNSASAAGGTKTEGSSSSATDMPVMSVSKMPEELSDKLPDSEVKEMVERNLNVLIEENSLDVELEEDPATAPPVFFREVIFSPALPICFDYHGRRIELSRGPVTGLIMGLAQLQGSGINLREIVNRRGILGWNKLCEFLAKEWLKDIKRNQLPNILSGIGPTNAVLQLFQGIYDLFRLPIEQYNKDGRIIRGFQLGAQSFTARTALAALEITSRIIHLLQFTAETTFDMLSAGPSMKKRKGGRQGKRRRQGRPKDLREGVANAYTIVREGINESANTLIEAAITEHDQKGYSGAVGAVVRQIPQLVVCPAVLATQATTNILGGAKSSLIPEAKLEARDKWKQEIH

Target ORF information:

RefSeq Version	NM_139491.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Autophagy-related 2 (Atg2), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139491.2

ORF Insert Sequence:

ATGTCGTGGT TCAATCCCTG GGATGGCCTC AAAACGAAAA TGTGTCGCTA CCTGCTGCAG 
CGATACCTGG GCCAGTTTTT CGAGAATAAC TTGAACCTGG AGCAGCTGAA GGTGGACCTT 
TACAATGGCA AGGCTGTGGT GGAGGACATT TTCCTAAAGG TCAACGCCTT CAACGATCTC 
TTCGAGGACC AGGGCTGGGC CTTCGAGGTG GTCTCGGGCC ACATTGGCCG CCTGACTGTC 
GTGGTGCCGT GGAATGCGCT GATGACCAAC GACAGTAGCC TGGAGATCCA CAATCTAACC 
ATCACACTGA GGCCAGTCAC ACGCTACCAG AGCGGCACCA CCATGCTGGA GTCCATGTGG 
TCGTCGGTGA GCAGCTCGAT GCAAATGGCC GAGGAGTGCA TGAAGCAGGT GGATGACGAT 
GTGCCCTTCC TGAACCACAA TAACGCCCTG ATTGGGTTGG AGAAGTTCGC AGAGACCATC 
GACAACGTGC TCAACCGCAT ACGCGCCAAG CTGACAAATA CTACTTTGAA CATTGAGTAC 
ATACTGCCCA GATCGGACCG GAAGCTGGTG CTCTCCTTCC AGGCCAGCCA TGTGGAGTAT 
AAGAACAAGA CGGGCTACGA GATGCCCATG TCCACCTCCC AGGACACTGA AACCGAAGAA 
GATCCCAATA GCAGCTTCAA TGCACTGCCC ATGATAGCCA AGCACAATTT GGTGATCGAG 
GGACTTAGTT TGCACACCTC CGAAGTGCTT GATGTGATGG ATCACCAGTT TGGACCCACT 
CCCTACGAGC AGCTCTGCAA GATTGTGGAG CTCAAGGGCG CGCAAAACAT CCAGATCAAC 
ATCAAGCAGA CGGAAAATAT AGTGGGGCCC AAGGTCAGCC TAGAACTGAG TCTGGACGAC 
ATATACTTCA TGCTAACGCC GCGTCAAATT CACCTGCTCA TCGAGTTCTC AAAGGGCTTC 
AACTGCGGCG GTCAGCAGGA GCGACCCAAA AAGGGCAGAA GCTTGAAATC GGCTCCGCCC 
AGTGAGTATC AGATGCACAG TTTGCAGCAG CCCACCCGGA TGACGGGGAT TCTGGGCCAG 
AATGCAGACT GGTGTACAGG AATGTCAGAG GCGGATCCCT CCGAATATTC CGTTTACGGA 
AATCCCCCGA GGAGCGGTTA CGCACGGAGT CGAAAGATGA GCGAATCCAC AAACAGCATG 
GTCACCTCCT GTACCAATAC TCTGCAGCAG GAGTTGGGCT ACACAGAGAA GTCCGGCGAG 
ATCCTCAAGT TCAAGGTGCA GATCTCGAAA ATCTACGGAA TTGCTTTGCA CCAGGACATT 
CTTATCCAGA ACACGGCCAA TATCGACAGT TGCAGCACGG TTTTCGATCA CGCAAGTTAC 
CTTCGCTACT CGGACACTGC CAGTGGGTTC TTCTTGGGCA CGCTCCTGGA GAATCACATC 
CCGCTGCAAA ATCAGCAGTA CCTTCTGCTA CGAGCAGCCC CAATAATCGT TAGTGGAAGC 
CAGCAGCGCT ATTTCCAGGA GCTCACATCG AGAACTACTC TCTCCGCCAC TGCGGTGGAT 
CTCATCGAGG TCCTGGACAA TTCCAAGGAG CATCTGCTTC AGTTCGACCG CCAGAGAAAC 
TGCCCGGATG GCTACCACAT TCGACCGGAG ATCGTGCTCA CCCAGAGCTC ATCGTTCATG 
TTCAAGCAGA ATCACAATCG TTGCTCTAAC AAAATTGAAT GCAGTTTGGA TGAATGTACG 
GCGGAATTGG ATATATCTAT TTACGATCGA CTAGGGGCGC TCTTTGGGAG CTCTCCGTTT 
TCCGGGGATT CGGCGTCGTC AACGCCCTAT CCAGATGATC CCAACCAAAC GGAGTTTGTG 
GTGAAGTCCG AAAATCTCCG TCTTCACTTG CGTTTCCCTG TGGTGGATGG CAGAGCCGCC 
AACGATCCGC TAAAGATTCC CTGGTGGAAG AAGAATGTGC GACGTGATTT CTTGGCAGTG 
GAGTTCCGCT CCCTGGTGGT TAGTTCGAGG TCTCAGATAA GCATCGTTTC GGATGAACTA 
GATGTGTTCT ATTGTGACGC GGATAAGCAG AGTGCCGTGC ATCTCCTGAA GTGCCAGCAA 
AAAATGCAGC CGAACAAGAA GATCCAAATT GACGTACTGC AGCTAAAGGA CACCTCGGAT 
GGCCAAAAGA AGGCAAGGAA GTCTCCGTTT AGCTCAAAAT GTACCTTTGG CTACACCTCC 
CATGTGGAGG GACTTGATGG TAGCGTGGAC AAAACCGATT CCCTTTTACC CGGCGAAACG 
GAGGAGATCA ATGAATTCTG CGACAGTTGC ACACAGTCCT CCAAGCTGAA GATATTTGCG 
TTTATACCGC ACGTAAAAGT TGTGCTGGAA TCCAAGAGCA TGTACGAACT GATATACAAC 
CGCTTGAACG GAGATCTCTT TATGTGGGAA CCACGTTCTC CGCACTATGA CATCAGCCCG 
GATAACGAAG TCCCAGAGTC AAAAGACCTG TACGCAGAGC CACAGCTGGA AGAGTTTAGA 
CGAAACCTGT TGCTCAGTAC GAGCGCCATG GAAAGTAGTA TTTACTTTTC CATGGCGGAG 
CCAACGGCTT CTGCTCCACC GGCGCCACCG GTGCGTAATG AAGCCTTCTC CTTTGAGTTG 
TTTGTAGAGC AGGGAACTTT GATTCTGTTC TCGCATCTTC TGGATCCCGA GACCAACCAG 
CGATCCAAGG AGTGCGGTAA GTTTCAGGTG AACTTAAAGG AGCTGCGTTT GTTTACAGTG 
AATGGCTTGG ACAACGATTC GACTCGGAGT TTCTTTTCCA TCCAACTGGG CGAGATCGAT 
GCCTTGCATT GCGGGAATAC GCTGCAGGAT TTGGAGCTGG CTTGGAGTGA TTTGGCGGAT 
GAAAAGCTGC TGCCCACCAT CTATAACTTT GCAAGTGTTC ATCAGCAGCA GGACAGGAAA 
AGAATGGAGG TTTTATCCCT CGTGGCTGAG ATCAAGAAGC AACCGGAGCA GCGCATCAAG 
AGAATGAAGA TGTCCTTCGG GATATGTGGC GCCATTCTGC AGCATCACTC CTGCCACTCG 
GAGCATTCTT GGCTGAATCA ACTGATCGAC TTTATGGACG TGGCCGACTT TCCCATCGAG 
GAATACGAAC CGTTTGCTCT GGTCTCCGAG CTGCAGTTGC ATGTGTGGAA TGCTGGGATC 
GACTATCGCC CGAAATTCTT TCCACAACGT GCGTTCCTGG ATCTGGGCTA CTGTACCTTG 
AGCAGCAATA TCATATCATC GATGAGTGGC TGCACCTTGC GATTGCTGGC CGAGGATTGT 
GTCCTGCAAC TGGGATTGGT TAAGGAGGAG TCCAACGACT CCCTGATACC TGTGCTGAAT 
CTGGGACTCT TGAATATATC CTTCCGTTTG AATGCAGAAG GTAGTGGACA ACCGCGAGTG 
GATTTGCGCT CTTCCATCCA CGATATGCAT CTGAAGACTT GCTACGATTC GGCAGCAGCT 
TTGGCCCAAC TTATAGCCTA TGTGGCCAAC GACAGGGACT TGTGTCCGCC AGAGGAGCCC 
ATCTTGAATG AGGACACTAA GCCTGCAGAG CCGAAACCCA ACGAGGAGCA TGAGATGAGC 
GATCACACCC AGAACCATGT GAGCAAATTG ATGGCCGATG CTGTGATAGA CGTAGAGTCC 
TCGCCCAATC TCAGGGCAAT GAAATCTCCA GGGGGTCGGG AGGAGGGCAT TGAGATATTC 
TACTTCCCGG ATGAGCCCAA AGAGAAACGG AAATCCACGT CCGCCAAGCT GCAGACCAAG 
TCACTGATGG TCGAGAGTCC CTCGGTCATC GGAGACATCC TGGACTTTGA ATCGAATGTG 
ATCTTGCAGT CCTACTACCA CCAGAGCCAA GTGCAACCGC AGACATCACT GGGCTCCCAG 
GTGCAGCAGG AGCTGGGTTC CCTGGGCAAC GCGTCCCTGG AGGAGCGGGA TTTCGACATC 
ATACACGACG AGGAGATTTC ACGCATGGAC AAGTTCGGTG TCAAGCAAAT TTACATCTCC 
GAGGACCCTT TGCAGATTGT GGACAATCAC TTTGAGCTGC CGCATGAGAA AGTGGACCTG 
CTTAGGCCTC CATCTAATTT CCCAGTGGCC GAGAGCACTT ACACCCTTTG CGAGATGACA 
TTCACATGGC ACCTTTATGG CGGCCGGGAT TTCCCCGATG AACCACTCAA GAAATCCTCT 
TCGTCGGCAA CCAGTGGCTT TGGCATGTCC GATACCTACA AGTATGGCGT GTCTCAAGCT 
CAAGAGCAGG ATAAACAGGA CAAAAAGGGT TCCAAGAAGG GTCTGCTCAA ACAACCCAAA 
GGATCGCAGC GCAATCTGGA GGTTCTAGTG GAGATGCAGC TCTCCAAGAT TCGATTCTCC 
TACGAAACCT ATCCGTTGAC CTCCATGTAC TCGTCCCGTC AGGTGCTCCT TGTGTCGGAA 
ATTGAAATAA GAGATAGGTT GCGCTCCTCG GACATTAACA AGTTTCTGTA CCATCCGACG 
GGCAACAATT TATCCCATAA GCCGGACGAG AACATGGTCA TAGTGAAGGC TCTGAATGTG 
CGTCCGAATC CGCAAAAGAG CTCCGCGGAA GAGTGCTCCC TGAGGGTCTC CATTTTGCCC 
ATAAAACTCA ACATTGACCA GGATACTCTG CTCTTTCTCG AGGACTTCTT TTCGGGCATG 
TTCCGAAACT CCGCTAGTGC TGCTGGTGGC ACCAAAACGG AGGGCAGCAG TTCGTCGGCC 
ACGGATATGC CGGTGATGTC GGTGAGCAAA ATGCCGGAGG AGTTATCCGA TAAATTGCCC 
GATTCGGAAG TTAAGGAGAT GGTGGAGAGG AACCTGAATG TGCTGATTGA AGAGAACAGC 
TTGGATGTGG AGTTGGAGGA GGATCCTGCC ACGGCTCCAC CTGTGTTCTT CCGCGAGGTG 
ATCTTCAGTC CAGCACTACC CATTTGCTTT GACTATCATG GACGGCGGAT TGAACTCTCC 
CGCGGACCGG TCACCGGCCT AATCATGGGT CTTGCCCAGC TGCAGGGATC CGGAATTAAT 
TTGAGGGAAA TTGTGAACCG CAGAGGCATT CTGGGATGGA ACAAACTGTG CGAGTTCCTG 
GCCAAGGAGT GGCTGAAGGA CATCAAGCGG AATCAATTGC CCAATATCCT AAGCGGGATT 
GGACCCACGA ATGCGGTGCT GCAGCTTTTC CAGGGTATCT ACGATCTTTT CCGGCTGCCC 
ATCGAGCAGT ACAACAAGGA TGGACGAATT ATCAGGGGAT TCCAGTTGGG TGCCCAGAGT 
TTCACCGCTC GCACAGCGCT GGCTGCCTTG GAGATCACCT CCAGGATTAT ACACCTGCTG 
CAATTCACGG CGGAGACTAC CTTCGATATG CTATCTGCAG GTCCTTCCAT GAAGAAGCGG 
AAGGGCGGCA GGCAGGGCAA ACGGAGGCGA CAGGGACGAC CCAAGGACCT GCGCGAGGGC 
GTGGCCAACG CCTATACCAT AGTGAGAGAG GGCATCAACG AATCGGCCAA CACACTCATC 
GAAGCCGCCA TCACGGAGCA CGACCAGAAG GGCTACAGCG GAGCGGTGGG CGCGGTGGTA 
CGCCAGATCC CTCAGCTGGT CGTCTGCCCG GCGGTGCTGG CCACCCAGGC CACCACCAAC 
ATCCTGGGCG GCGCCAAGAG CTCGCTGATT CCGGAGGCCA AACTGGAGGC GCGCGACAAG 
TGGAAGCAGG AGATCCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Atg2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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