ecd cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ecd
Entrez Gene ID	38291
Full Name	ecdysoneless
Synonyms	CG5714,Dmel\CG5714,Ed,ecd-1,ecd1,ecdl,l(3)62Db,l(3)ecd,l(3)ecd-1,l(3)ecd1
General protein information	Preferred Names ecdysoneless Names CG5714 gene product from transcript CG5714-RA CG5714-PA ecd-PA ecdysone echinoid
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 62D4-62D4\|3-1.3 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_139450.3	NP_647707.1	ecdysoneless

Pathways from BioSystems

Homologies

	ECD	XP_001103459.1
Canis lupus familiaris (dog)	ECD	XP_536386.1
Bos taurus (cattle)	ECD	NP_001039736.1
Rattus norvegicus (Norway rat)	Ecd	XP_006251737.1
Arabidopsis thaliana (thale cress)	AT5G65490	NP_201352.1
Oryza sativa (rice)	Os08g0447200	NP_001061930.1
Homo sapiens (human)	ECD	NP_009196.1
Pan troglodytes (chimpanzee)	ECD	XP_001141366.2
Gallus gallus (chicken)	ECD	NP_001186453.1
Mus musculus (house mouse)	Ecd	NP_081751.1
Drosophila melanogaster (fruit fly)	ecd	NP_647707.1
Danio rerio (zebrafish)	ecd	NP_956185.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005814	XP_315835.4
Xenopus tropicalis (tropical clawed frog)	ecd	XP_002936899.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In Drosophila cells, Ecd directly interacts with core components of the U5 snRNP spliceosomal complex.
Title: Unexpected role of the steroid-deficiency protein ecdysoneless in pre-mRNA splicing.

The transcription factor Grainy head and the steroid hormone ecdysone drive the production of two partially overlapping sets of cuticle factors.
Title: The transcription factor Grainy head and the steroid hormone ecdysone cooperate during differentiation of the skin of Drosophila melanogaster.

The following ecd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ecd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17281	NM_139450.3 Latest version!	Drosophila melanogaster ecdysoneless (ecd), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ecd ( NM_139450.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_647707.1 Drosophila melanogaster ecdysoneless (ecd), mRNA.

Price & Availability↑

CloneID	OFa17281
Clone ID Related Accession (Same CDS sequence)	NM_139450.3
Accession Version	NM_139450.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ecdysoneless
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_139450.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAAGA TTCCAGGCAG CAATCTGGAG TTCGTCCGCG AGGAGGACTT CGTGGAGTAC 
TACATCTTTC CCAAGATCCC CGACAACGTA CAGGATGAGG GCGCGCTGCG CAAACTGATG 
CTTCAGATCC GCAACGAGAT CTCGGCCATT GTAAGGGAAA AGTCCTTAGA GCGCAGCTAT 
CTGTGGCACA AGGACGAGTT TCAGCTTCAG GTGAGGCTGG GCGGCGCCGA GGAGCGCCTG 
CTGAACGAGG AGACAAACCC GGAGGAGGAG GCGGAACTGG GCGACCTCCC ACCGCACTTC 
CACGGCGTAA CCCACTATGG CGACAACATC AGCGACGAGT GGTTCGTTGT CTATCTGCTC 
ACGGAGATCA CACGTGCACG CGGCGACTGC ATTGCACGGG TGAGCGATTC GGATGGCGAG 
TTCCTGCTCA TCGAGGCGGC GGACGCCCTG CCCGACTGGG CGTCTCCGGA AACGTGCGAG 
CAGCGCGTTT ATCTGGTCGG TGGTCACCTG CAGCTTCTCC AGAACTCCGC CGCCAGCAGC 
CAGGACAAGC CACTGACCAT GGCCATGGCT GTGCAACGTA TACGAATGAA TCCCACCCTG 
TACCGCTGCT CGCAGGAGAT ACAGTCGTGC ATTGACGCGC GGCTTAAGGA GTACCAGATC 
GCTCAGCCGC ACTTCTCCAT TCATCGCCAG GTGCTGGAGC TCCCGCATAG CGCTGCACAG 
CTTCTGAAAC AGAAGCCAAG GCTGCTCTCC TCGGCGGTTA GGGCTTTCTG CGAGCGGGAC 
TCGCTGGACA TCAAGGCACT TCGCACCATG CGATATTTCC CACCGGAGGC CACACGCGTG 
CGCACCAACG TGCGTTTCAC CCGCTGTCTG TACGCCATGT TGTCGCACCA GCAGTATCTG 
CCCGAGAAGA GACTCGGCTG GCATCTCACG GATCCTGTAT CCGAGCCGGA ACGATATAAG 
GAACAACTAT TGGGCTTGAA GCTGGCCAGT GGACTGGAGA TCCTGGCCAC TCAAGCCAAG 
CGGGTTGAGG GCCAACAGCT GGAGGACCTT CCTGCCTGGC GATCGTATCT GCGGAGCTTG 
CTGAGCAAGG GCTACTTCCG GGACAACATC GAGGGCAGTG CCGAGTACCA GGAGCTGCTG 
AACAAGGCCA AGGTGTACTT TCGTGGCAAC CAGGAGCGTT TCCGCACAGC CTCCAGAGCG 
GGTGCCGAGA TCCTCGACCT GTTGCTCCAT CCAGCGGAGG CTGCTTCCGA GGAGCTGCGC 
GATGAGGAGA ACAACCTGCA GCCCAGCGAC TCGGATGAGT GGTTGAATAT CAGCGCTGAG 
GATCTAGACT CCATGCTGCA GGATCGCTAC GGACCCAAGA AGCTCTACAA GCCGAACGGA 
CAAATGAATG CCGAGGAGTT CACCAAGCAG CTGGCGGAGT TCTTAGATCG GCAGTCCAAC 
TACGAGGGCA TTGAGCACCG AGGGCTCGAG GAGCCAGAAC TGGACTCAGA CGATGACGAG 
CCGCCGCCGC AGGCAAACGG TTCCACAGGA CTGACAGCAA AGGTCAAGAA GAATCCCTCC 
ATGCGCAAGG CGTGCCAGCG CAATTCCGTC ATCCAGCCGG AGGAACCGGA CAGCACCCAT 
GTGCGGAATT TCCTGGATTT CGTAATACCC GAGGACAACT GGGACTCCAC CTCGGAGATG 
AGCGACTACG CGGACGAGGA CGACATGGAG AGTAATCTAA ATGCACTTTC TGGAGGCGGC 
AGTGTGTTCC CACTCGATCG ACAGATTCAA TCGTATATGG AGCAGATGGA CCGCGAGTTG 
GCTCAGACCA GCGTGGGCAA GTCCTTCCAC GGCAAGAAGA AGACAGCTCC ACAGGCGGAC 
GAGGACGATT TCGATGACAT TGAGGACTTT GAGCCCATCA ATATAAACGT AAACACGCTG 
CGCAACATGA TGGATAGCTA CCAATCGCAG GTTGGCGGCG CAGGTCCTGT ATCCAATCTG 
TTTAGCGCCA TGGGCGTGGG CATGTCGGCT GTCGAGGACA AGGAACAAAA GGATATATCT 
GAGTCGGCCG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_647707.1
CDS	83..2137
Translation	MSKIPGSNLEFVREEDFVEYYIFPKIPDNVQDEGALRKLMLQIRNEISAIVREKSLERSYLWHKDEFQLQVRLGGAEERLLNEETNPEEEAELGDLPPHFHGVTHYGDNISDEWFVVYLLTEITRARGDCIARVSDSDGEFLLIEAADALPDWASPETCEQRVYLVGGHLQLLQNSAASSQDKPLTMAMAVQRIRMNPTLYRCSQEIQSCIDARLKEYQIAQPHFSIHRQVLELPHSAAQLLKQKPRLLSSAVRAFCERDSLDIKALRTMRYFPPEATRVRTNVRFTRCLYAMLSHQQYLPEKRLGWHLTDPVSEPERYKEQLLGLKLASGLEILATQAKRVEGQQLEDLPAWRSYLRSLLSKGYFRDNIEGSAEYQELLNKAKVYFRGNQERFRTASRAGAEILDLLLHPAEAASEELRDEENNLQPSDSDEWLNISAEDLDSMLQDRYGPKKLYKPNGQMNAEEFTKQLAEFLDRQSNYEGIEHRGLEEPELDSDDDEPPPQANGSTGLTAKVKKNPSMRKACQRNSVIQPEEPDSTHVRNFLDFVIPEDNWDSTSEMSDYADEDDMESNLNALSGGGSVFPLDRQIQSYMEQMDRELAQTSVGKSFHGKKKTAPQADEDDFDDIEDFEPININVNTLRNMMDSYQSQVGGAGPVSNLFSAMGVGMSAVEDKEQKDISESAV

Target ORF information:

RefSeq Version	NM_139450.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ecdysoneless (ecd), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139450.3

ORF Insert Sequence:

ATGAGCAAGA TTCCAGGCAG CAATCTGGAG TTCGTCCGCG AGGAGGACTT CGTGGAGTAC 
TACATCTTTC CCAAGATCCC CGACAACGTA CAGGATGAGG GCGCGCTGCG CAAACTGATG 
CTTCAGATCC GCAACGAGAT CTCGGCCATT GTAAGGGAAA AGTCCTTAGA GCGCAGCTAT 
CTGTGGCACA AGGACGAGTT TCAGCTTCAG GTGAGGCTGG GCGGCGCCGA GGAGCGCCTG 
CTGAACGAGG AGACAAACCC GGAGGAGGAG GCGGAACTGG GCGACCTCCC ACCGCACTTC 
CACGGCGTAA CCCACTATGG CGACAACATC AGCGACGAGT GGTTCGTTGT CTATCTGCTC 
ACGGAGATCA CACGTGCACG CGGCGACTGC ATTGCACGGG TGAGCGATTC GGATGGCGAG 
TTCCTGCTCA TCGAGGCGGC GGACGCCCTG CCCGACTGGG CGTCTCCGGA AACGTGCGAG 
CAGCGCGTTT ATCTGGTCGG TGGTCACCTG CAGCTTCTCC AGAACTCCGC CGCCAGCAGC 
CAGGACAAGC CACTGACCAT GGCCATGGCT GTGCAACGTA TACGAATGAA TCCCACCCTG 
TACCGCTGCT CGCAGGAGAT ACAGTCGTGC ATTGACGCGC GGCTTAAGGA GTACCAGATC 
GCTCAGCCGC ACTTCTCCAT TCATCGCCAG GTGCTGGAGC TCCCGCATAG CGCTGCACAG 
CTTCTGAAAC AGAAGCCAAG GCTGCTCTCC TCGGCGGTTA GGGCTTTCTG CGAGCGGGAC 
TCGCTGGACA TCAAGGCACT TCGCACCATG CGATATTTCC CACCGGAGGC CACACGCGTG 
CGCACCAACG TGCGTTTCAC CCGCTGTCTG TACGCCATGT TGTCGCACCA GCAGTATCTG 
CCCGAGAAGA GACTCGGCTG GCATCTCACG GATCCTGTAT CCGAGCCGGA ACGATATAAG 
GAACAACTAT TGGGCTTGAA GCTGGCCAGT GGACTGGAGA TCCTGGCCAC TCAAGCCAAG 
CGGGTTGAGG GCCAACAGCT GGAGGACCTT CCTGCCTGGC GATCGTATCT GCGGAGCTTG 
CTGAGCAAGG GCTACTTCCG GGACAACATC GAGGGCAGTG CCGAGTACCA GGAGCTGCTG 
AACAAGGCCA AGGTGTACTT TCGTGGCAAC CAGGAGCGTT TCCGCACAGC CTCCAGAGCG 
GGTGCCGAGA TCCTCGACCT GTTGCTCCAT CCAGCGGAGG CTGCTTCCGA GGAGCTGCGC 
GATGAGGAGA ACAACCTGCA GCCCAGCGAC TCGGATGAGT GGTTGAATAT CAGCGCTGAG 
GATCTAGACT CCATGCTGCA GGATCGCTAC GGACCCAAGA AGCTCTACAA GCCGAACGGA 
CAAATGAATG CCGAGGAGTT CACCAAGCAG CTGGCGGAGT TCTTAGATCG GCAGTCCAAC 
TACGAGGGCA TTGAGCACCG AGGGCTCGAG GAGCCAGAAC TGGACTCAGA CGATGACGAG 
CCGCCGCCGC AGGCAAACGG TTCCACAGGA CTGACAGCAA AGGTCAAGAA GAATCCCTCC 
ATGCGCAAGG CGTGCCAGCG CAATTCCGTC ATCCAGCCGG AGGAACCGGA CAGCACCCAT 
GTGCGGAATT TCCTGGATTT CGTAATACCC GAGGACAACT GGGACTCCAC CTCGGAGATG 
AGCGACTACG CGGACGAGGA CGACATGGAG AGTAATCTAA ATGCACTTTC TGGAGGCGGC 
AGTGTGTTCC CACTCGATCG ACAGATTCAA TCGTATATGG AGCAGATGGA CCGCGAGTTG 
GCTCAGACCA GCGTGGGCAA GTCCTTCCAC GGCAAGAAGA AGACAGCTCC ACAGGCGGAC 
GAGGACGATT TCGATGACAT TGAGGACTTT GAGCCCATCA ATATAAACGT AAACACGCTG 
CGCAACATGA TGGATAGCTA CCAATCGCAG GTTGGCGGCG CAGGTCCTGT ATCCAATCTG 
TTTAGCGCCA TGGGCGTGGG CATGTCGGCT GTCGAGGACA AGGAACAAAA GGATATATCT 
GAGTCGGCCG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ecd cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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