mu2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	mu2
Entrez Gene ID	38257
Full Name	mutator 2
Synonyms	CG1960,Dmel\CG1960,MDC1,MU2,Mdc1,Mu2,cDNA D,mu-2,mutator[2]
General protein information	Preferred Names mutator 2 Names CG1960 gene product from transcript CG1960-RB CG1960-PA CG1960-PB CG1960-PC mu2-PA mu2-PB mu2-PC mutator2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 62B11-62B11

mRNA and Protein(s)

mRNA	Protein	Name
NM_079163.4	NP_523887.2	mutator 2, isoform A
NM_167927.3	NP_728695.1	mutator 2, isoform B
NM_001169855.2	NP_001163326.1	mutator 2, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

As compared to the MDC1 forkhead-associated FHA) domain, the MU2 FHA domain dimerizes using a different and more stable interface and contains a degenerate phosphothreonine-binding pocket.
Title: Dimerization, but not phosphothreonine binding, is conserved between the forkhead-associated domains of Drosophila MU2 and human MDC1.

The interplay between MU2 and HP1a is dynamic and may be different in euchromatin and heterochromatin during DNA break recognition and repair.
Title: MU2 and HP1a regulate the recognition of double strand breaks in Drosophila melanogaster.

MU2 participates at an early stage in the recognition of DNA damage at a step that is prerequisite for both DNA repair and cell cycle checkpoint control.
Title: Recognition of double strand breaks by a mutator protein (MU2) in Drosophila melanogaster.

The following mu2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mu2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17253	NM_167927.3 Latest version!	Drosophila melanogaster mutator 2 (mu2), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa17254	NM_079163.4 Latest version!	Drosophila melanogaster mutator 2 (mu2), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa20446	NM_001169855.2 Latest version!	Drosophila melanogaster mutator 2 (mu2), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mu2 ( NM_167927.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728695.1 Drosophila melanogaster mutator 2 (mu2), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa17253
Clone ID Related Accession (Same CDS sequence)	NM_167927.3
Accession Version	NM_167927.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mutator 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_167927.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGATG TAAGCCTGTT TTTTGGCGGT TTGCCGGCCA TTCTTCTGAA AGCGGATACC 
ATTTACCGCA TTGGGAGGCA GAAGGGCCTG GAAATAAGCA TCGCCGATGA GTCAATGGAG 
CTGGCCCACG CCACAGCCTG CATACTGCGC CGAGGTGTGG TGCGCCTGGC TGCCCTGGTG 
GGCAAGATCT TTGTGAACGA CCAGGAGGAG ACCGTGGTGG ACATCGGCAT GGAGAACGCC 
GTGGCCGGCA AGGTGAAACT CCGCTTTGGC AATGTGGAGG CCAGGCTAGA GTTTGGAGAA 
GATCACGATG AGGTCCACGA CAGTAGCGGA TTCGGGGAAT GCCTGAAGAA CGGATTAAAC 
AACACAACCT TGGACAGCAT GGATGTTCCG GAAACACAGC CTCCATCCGC CAACACATCA 
GTCAACACAA CGGCAGATAG CTTATTTATA CCCGAGACCC AAGCGGTGTT ATGCGAAAGG 
CCTTCGACCG GACAAAGAGT GTCGCTGGGC GATGACTTCA TGATACCCGA AACGCAGGAT 
ATGCTTGCGG ATCTTCCACC TGAACCACCA GTGTTTAAAC CCCCGGTTAT TCCCGTAGTT 
GATAAGCCAG GTCCGACGAT AGACAGCGAT GAGGAATCAT CTCAGGGCAG CATTATACGC 
ATGTGCACCC AGGATTATAA TGAGGATGCC ATCGATGACT TCGATACCTC ACAGGTACTC 
TGCGATGTTC TGCTGCCTCT TCCGCCGCCA ACAGCACCAA TTGAAGGTCT GGAAAATCAG 
GATACTAAGC GGGATCAACT GGACACCACC GACATGGAAA TGAGTGCCTT GAACTGGTCG 
GCTAGCAATT CCAAATGCTG TGCTCTGAGT AGCACCAAAG CGGATGATAT ATTGCCGCGT 
GGCGATGCCT GCATTACTCC CGATTTGACC GCTCCGTCCG TAGATAGAAA TATCTGCACA 
CCCGATCTAT TTGACCTGAT CATGGGGGGG GATAGGAGGC GCGATGGCTC CAGCAGTCCA 
GATCCGTTCG TTCGTCCTGC GGACAATACA AATACCGCCA CACCGCAGTT TGGCGGAGTT 
AAACAGCTGG TTGTGGCGAC GATTGAGACC CCCACTGCCA CTCCTGACAC GAATAGTCAG 
GAAAAGAATC AAGATATGAT AGCCACTCAA CGATTTCCAC GACATAAATT GTTGGAAAGT 
GACGAGGAAG ACAATGGGCA AAACAACCAA GACTTCGTGG CTACTCAGGC GTTTAACTTA 
GGACGTCCGC AGGCACCACA AGAAAATGAA GCCAATCAAG ATCTCATAGC GACACAGGCA 
TTTAACTTGG CACCGCCACA AAAAGTTGAT TCACCTAAAC CCTCTACAGC TAATGATGCT 
TCCAACCAAG ATTTTATAGA AACTCAGGCA TTTAACTTAG GAATCCCACA AGCAAACATG 
GCTTCACCAG AAAATAATGA GATTTCCAAC CAAGATTTGA TAGCCACTCA AGCATTTCCC 
GCCAAGATCA ATAGCTCTCG TTGCCAGGAC TTCGTAGCCA CGCAACCTTT TCATGTGGTC 
AATCAGCATT CCGTGAGTTT ACAGGACAAG GAGAACATTC CACTTGACGA CAGTGTCAAA 
GTTGCATCAG GGACTGTGGC AACGTCGCCT GTAAGTCCCA ATGAGGAGTC GATACCATTT 
TTTGAGCCGT GTGTGATCAA GGATAAGAAT CACTATCAGA AGATCTGTCA GATTGAAGGC 
GTTTTTAGTA ACGTGAGTAA CAGGAGTCGT GGGCGTACTG ATGGAACTGG CTTTGGCCGT 
CTCAAACGGG TGGCCAAATC GGAATCTCCT CCAGAAACCC CAATCCGTAA GGACAGACGA 
AGATCGGAGG GTTCTGAGAG ACCGGGAAGT AACTCGAGAG AACGGCTAAA CAATTTAGTG 
GATAAAAAAT CGGAAGCCCT TAACAAGTCG GTTATCCGGG AACATGGCAT CGATTCCATT 
CCAGAAGAGA TAACAAAGGA AGACAAATCA GACTCAGAAG AGAAAGGCGA TTGTTTCAAC 
AATGTTGTTA ATCAATGGCG CTCCCGTAAA AGACGAATTC AAAGCCGCCC TGGTACTCCA 
GGAAGCTCTT CAGAAAGGAA TGAAGTCAAC GCAGATGTGG TGGATAAACC CAACGAAGAG 
CCACCTACCA AAAATATACG TGCTAGAAAG GCCAAGAAGG CGGAAAAGGC AGACTCATCC 
AAGGATATTC CCAAGCCTAC GACAAGAACC AGGCGCCAAA CCAGCGATGA AGATGTTCGA 
ACGCCTGATC TAGGAAAAGG AAGAAAGGCC AAGAACACTG CTTCGAGTAA GGCAGACACA 
TCTAAGAATA TTGCCAAAGG CTCGAAGAGA GTCACTCGAC AAAATAGCGC TGATGATGCC 
GAGTTGCTTG AGGAGGACAG TTCTGTCCAG ACAGAAAGCG GAAAAAGGGT TAAGAAGGTT 
AAGTCAAGTA AAGGGGAAAC GTCAAAGGAC ATTCCCAAGG CAAAACCCAG AACTAGGCGC 
CAAACTGCCG ACGAAGATGC TATGACCCCA AAGGCAGAAA AAGAAAGACA GATTCAGGGG 
GAAGAAACCA AGGATATGTC CAAGCCAGCG GCCAGAACCA GGCGAAAAGC TAGCGCTGAA 
GAGACTGAGA GTGCAGCACA AGCCGTCAAA CCGAAAAGAG GTGGAAAAGG CAAGAAAAAC 
GAGACTACCA AAGCTGAGGC AACTGTGGAA AAGCCTATGG TTAGAACCAG GCGCATGACA 
ATCGCCGAAG ATTCCAGCAC CCTGACGGAA GTAAAAACAG GGGGCGCATT AATTAAACGT 
GCGGTTGTCC GCATATCCAG GGTAAGCATA GAACAACTCG AGTCTTCCAC AAGCGCTGGA 
AATAATGCCA GAGTAACTGG GGCTGCTCAG TCGCGTTCTA CAACGCCCTC AATTGAAGAG 
CCATCATCTA GCTCAGCTGC TGCCAAGGAG CCAAATGTAT CTACAGCAAC TACACGCAGG 
CGAGCTAAGC GCTTAACATC TGGCGAAGAG CCCACGTCCA GCTCGGACGT CCCTACCAAG 
AAGCCTAAAA TGTCTGTTGA CGAGGAAGTT CTGAAGAACA GATCAGGCGC TACTACAACG 
CGATTCAGTC AGCCGGATGC CGATCCCCTT AGAGCAATTA ACTTGTACGT GAGAAAGGCC 
AAGACTACTA CGGGCAAAAT TAAGGTAGCG TTCACCATGT GCAATCGTCC AGCTTTGGAA 
ACTGTCCTCA AGTCACTGAA GCACGTGGTG GAGATCACCG AAGATCCCTT GCAGTGCGAC 
CTGCTTGTGA TGGACAAGGG CGAGCGCACC TATAAGTTCC TAACAGTCAT TGCTTCCAAC 
AAACCAGTTC TCTCCACCAA TTGGCTGCAC TCTGTGAAGA AGACCAGGAG CATCGACATA 
AAGGCAGACC ATCTGTTCAG CGACCCAACG TTCGAGGAGA CCTACAAGTT CAAACCCTCC 
TCCGTACTGG AACACCCTCG CTTGCTGTAT GGCTTGCACT TTATGCTCGG CAAAGACATA 
GTGCCAAAGG CCAACGAAAT GAAAGTCATC ATTCACAGCG CCGGCGGCAA GGTTCATGCC 
CAACCGCCTT CGCTGGCCAT CAGCGTGGAT CTGTACGTGG TCACCACCAG CAAGGATACG 
AAATCTAAGC GACGTCTGAA CAACTATGAA AAGGTGCACT TCATCAAAAC CGAGGCTGTT 
ATGCAGGCGC TCGTTCAACA CAACATTGAA ATGCTACAGG AGCACACGCT CAAACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728695.1
CDS	27..3806
Translation	MADVSLFFGGLPAILLKADTIYRIGRQKGLEISIADESMELAHATACILRRGVVRLAALVGKIFVNDQEETVVDIGMENAVAGKVKLRFGNVEARLEFGEDHDEVHDSSGFGECLKNGLNNTTLDSMDVPETQPPSANTSVNTTADSLFIPETQAVLCERPSTGQRVSLGDDFMIPETQDMLADLPPEPPVFKPPVIPVVDKPGPTIDSDEESSQGSIIRMCTQDYNEDAIDDFDTSQVLCDVLLPLPPPTAPIEGLENQDTKRDQLDTTDMEMSALNWSASNSKCCALSSTKADDILPRGDACITPDLTAPSVDRNICTPDLFDLIMGGDRRRDGSSSPDPFVRPADNTNTATPQFGGVKQLVVATIETPTATPDTNSQEKNQDMIATQRFPRHKLLESDEEDNGQNNQDFVATQAFNLGRPQAPQENEANQDLIATQAFNLAPPQKVDSPKPSTANDASNQDFIETQAFNLGIPQANMASPENNEISNQDLIATQAFPAKINSSRCQDFVATQPFHVVNQHSVSLQDKENIPLDDSVKVASGTVATSPVSPNEESIPFFEPCVIKDKNHYQKICQIEGVFSNVSNRSRGRTDGTGFGRLKRVAKSESPPETPIRKDRRRSEGSERPGSNSRERLNNLVDKKSEALNKSVIREHGIDSIPEEITKEDKSDSEEKGDCFNNVVNQWRSRKRRIQSRPGTPGSSSERNEVNADVVDKPNEEPPTKNIRARKAKKAEKADSSKDIPKPTTRTRRQTSDEDVRTPDLGKGRKAKNTASSKADTSKNIAKGSKRVTRQNSADDAELLEEDSSVQTESGKRVKKVKSSKGETSKDIPKAKPRTRRQTADEDAMTPKAEKERQIQGEETKDMSKPAARTRRKASAEETESAAQAVKPKRGGKGKKNETTKAEATVEKPMVRTRRMTIAEDSSTLTEVKTGGALIKRAVVRISRVSIEQLESSTSAGNNARVTGAAQSRSTTPSIEEPSSSSAAAKEPNVSTATTRRRAKRLTSGEEPTSSSDVPTKKPKMSVDEEVLKNRSGATTTRFSQPDADPLRAINLYVRKAKTTTGKIKVAFTMCNRPALETVLKSLKHVVEITEDPLQCDLLVMDKGERTYKFLTVIASNKPVLSTNWLHSVKKTRSIDIKADHLFSDPTFEETYKFKPSSVLEHPRLLYGLHFMLGKDIVPKANEMKVIIHSAGGKVHAQPPSLAISVDLYVVTTSKDTKSKRRLNNYEKVHFIKTEAVMQALVQHNIEMLQEHTLKL

Target ORF information:

RefSeq Version	NM_167927.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mutator 2 (mu2), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167927.3

ORF Insert Sequence:

ATGGCAGATG TAAGCCTGTT TTTTGGCGGT TTGCCGGCCA TTCTTCTGAA AGCGGATACC 
ATTTACCGCA TTGGGAGGCA GAAGGGCCTG GAAATAAGCA TCGCCGATGA GTCAATGGAG 
CTGGCCCACG CCACAGCCTG CATACTGCGC CGAGGTGTGG TGCGCCTGGC TGCCCTGGTG 
GGCAAGATCT TTGTGAACGA CCAGGAGGAG ACCGTGGTGG ACATCGGCAT GGAGAACGCC 
GTGGCCGGCA AGGTGAAACT CCGCTTTGGC AATGTGGAGG CCAGGCTAGA GTTTGGAGAA 
GATCACGATG AGGTCCACGA CAGTAGCGGA TTCGGGGAAT GCCTGAAGAA CGGATTAAAC 
AACACAACCT TGGACAGCAT GGATGTTCCG GAAACACAGC CTCCATCCGC CAACACATCA 
GTCAACACAA CGGCAGATAG CTTATTTATA CCCGAGACCC AAGCGGTGTT ATGCGAAAGG 
CCTTCGACCG GACAAAGAGT GTCGCTGGGC GATGACTTCA TGATACCCGA AACGCAGGAT 
ATGCTTGCGG ATCTTCCACC TGAACCACCA GTGTTTAAAC CCCCGGTTAT TCCCGTAGTT 
GATAAGCCAG GTCCGACGAT AGACAGCGAT GAGGAATCAT CTCAGGGCAG CATTATACGC 
ATGTGCACCC AGGATTATAA TGAGGATGCC ATCGATGACT TCGATACCTC ACAGGTACTC 
TGCGATGTTC TGCTGCCTCT TCCGCCGCCA ACAGCACCAA TTGAAGGTCT GGAAAATCAG 
GATACTAAGC GGGATCAACT GGACACCACC GACATGGAAA TGAGTGCCTT GAACTGGTCG 
GCTAGCAATT CCAAATGCTG TGCTCTGAGT AGCACCAAAG CGGATGATAT ATTGCCGCGT 
GGCGATGCCT GCATTACTCC CGATTTGACC GCTCCGTCCG TAGATAGAAA TATCTGCACA 
CCCGATCTAT TTGACCTGAT CATGGGGGGG GATAGGAGGC GCGATGGCTC CAGCAGTCCA 
GATCCGTTCG TTCGTCCTGC GGACAATACA AATACCGCCA CACCGCAGTT TGGCGGAGTT 
AAACAGCTGG TTGTGGCGAC GATTGAGACC CCCACTGCCA CTCCTGACAC GAATAGTCAG 
GAAAAGAATC AAGATATGAT AGCCACTCAA CGATTTCCAC GACATAAATT GTTGGAAAGT 
GACGAGGAAG ACAATGGGCA AAACAACCAA GACTTCGTGG CTACTCAGGC GTTTAACTTA 
GGACGTCCGC AGGCACCACA AGAAAATGAA GCCAATCAAG ATCTCATAGC GACACAGGCA 
TTTAACTTGG CACCGCCACA AAAAGTTGAT TCACCTAAAC CCTCTACAGC TAATGATGCT 
TCCAACCAAG ATTTTATAGA AACTCAGGCA TTTAACTTAG GAATCCCACA AGCAAACATG 
GCTTCACCAG AAAATAATGA GATTTCCAAC CAAGATTTGA TAGCCACTCA AGCATTTCCC 
GCCAAGATCA ATAGCTCTCG TTGCCAGGAC TTCGTAGCCA CGCAACCTTT TCATGTGGTC 
AATCAGCATT CCGTGAGTTT ACAGGACAAG GAGAACATTC CACTTGACGA CAGTGTCAAA 
GTTGCATCAG GGACTGTGGC AACGTCGCCT GTAAGTCCCA ATGAGGAGTC GATACCATTT 
TTTGAGCCGT GTGTGATCAA GGATAAGAAT CACTATCAGA AGATCTGTCA GATTGAAGGC 
GTTTTTAGTA ACGTGAGTAA CAGGAGTCGT GGGCGTACTG ATGGAACTGG CTTTGGCCGT 
CTCAAACGGG TGGCCAAATC GGAATCTCCT CCAGAAACCC CAATCCGTAA GGACAGACGA 
AGATCGGAGG GTTCTGAGAG ACCGGGAAGT AACTCGAGAG AACGGCTAAA CAATTTAGTG 
GATAAAAAAT CGGAAGCCCT TAACAAGTCG GTTATCCGGG AACATGGCAT CGATTCCATT 
CCAGAAGAGA TAACAAAGGA AGACAAATCA GACTCAGAAG AGAAAGGCGA TTGTTTCAAC 
AATGTTGTTA ATCAATGGCG CTCCCGTAAA AGACGAATTC AAAGCCGCCC TGGTACTCCA 
GGAAGCTCTT CAGAAAGGAA TGAAGTCAAC GCAGATGTGG TGGATAAACC CAACGAAGAG 
CCACCTACCA AAAATATACG TGCTAGAAAG GCCAAGAAGG CGGAAAAGGC AGACTCATCC 
AAGGATATTC CCAAGCCTAC GACAAGAACC AGGCGCCAAA CCAGCGATGA AGATGTTCGA 
ACGCCTGATC TAGGAAAAGG AAGAAAGGCC AAGAACACTG CTTCGAGTAA GGCAGACACA 
TCTAAGAATA TTGCCAAAGG CTCGAAGAGA GTCACTCGAC AAAATAGCGC TGATGATGCC 
GAGTTGCTTG AGGAGGACAG TTCTGTCCAG ACAGAAAGCG GAAAAAGGGT TAAGAAGGTT 
AAGTCAAGTA AAGGGGAAAC GTCAAAGGAC ATTCCCAAGG CAAAACCCAG AACTAGGCGC 
CAAACTGCCG ACGAAGATGC TATGACCCCA AAGGCAGAAA AAGAAAGACA GATTCAGGGG 
GAAGAAACCA AGGATATGTC CAAGCCAGCG GCCAGAACCA GGCGAAAAGC TAGCGCTGAA 
GAGACTGAGA GTGCAGCACA AGCCGTCAAA CCGAAAAGAG GTGGAAAAGG CAAGAAAAAC 
GAGACTACCA AAGCTGAGGC AACTGTGGAA AAGCCTATGG TTAGAACCAG GCGCATGACA 
ATCGCCGAAG ATTCCAGCAC CCTGACGGAA GTAAAAACAG GGGGCGCATT AATTAAACGT 
GCGGTTGTCC GCATATCCAG GGTAAGCATA GAACAACTCG AGTCTTCCAC AAGCGCTGGA 
AATAATGCCA GAGTAACTGG GGCTGCTCAG TCGCGTTCTA CAACGCCCTC AATTGAAGAG 
CCATCATCTA GCTCAGCTGC TGCCAAGGAG CCAAATGTAT CTACAGCAAC TACACGCAGG 
CGAGCTAAGC GCTTAACATC TGGCGAAGAG CCCACGTCCA GCTCGGACGT CCCTACCAAG 
AAGCCTAAAA TGTCTGTTGA CGAGGAAGTT CTGAAGAACA GATCAGGCGC TACTACAACG 
CGATTCAGTC AGCCGGATGC CGATCCCCTT AGAGCAATTA ACTTGTACGT GAGAAAGGCC 
AAGACTACTA CGGGCAAAAT TAAGGTAGCG TTCACCATGT GCAATCGTCC AGCTTTGGAA 
ACTGTCCTCA AGTCACTGAA GCACGTGGTG GAGATCACCG AAGATCCCTT GCAGTGCGAC 
CTGCTTGTGA TGGACAAGGG CGAGCGCACC TATAAGTTCC TAACAGTCAT TGCTTCCAAC 
AAACCAGTTC TCTCCACCAA TTGGCTGCAC TCTGTGAAGA AGACCAGGAG CATCGACATA 
AAGGCAGACC ATCTGTTCAG CGACCCAACG TTCGAGGAGA CCTACAAGTT CAAACCCTCC 
TCCGTACTGG AACACCCTCG CTTGCTGTAT GGCTTGCACT TTATGCTCGG CAAAGACATA 
GTGCCAAAGG CCAACGAAAT GAAAGTCATC ATTCACAGCG CCGGCGGCAA GGTTCATGCC 
CAACCGCCTT CGCTGGCCAT CAGCGTGGAT CTGTACGTGG TCACCACCAG CAAGGATACG 
AAATCTAAGC GACGTCTGAA CAACTATGAA AAGGTGCACT TCATCAAAAC CGAGGCTGTT 
ATGCAGGCGC TCGTTCAACA CAACATTGAA ATGCTACAGG AGCACACGCT CAAACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mu2 ( NM_079163.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523887.2 Drosophila melanogaster mutator 2 (mu2), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa17254
Clone ID Related Accession (Same CDS sequence)	NM_079163.4
Accession Version	NM_079163.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mutator 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079163.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGATG TAAGCCTGTT TTTTGGCGGT TTGCCGGCCA TTCTTCTGAA AGCGGATACC 
ATTTACCGCA TTGGGAGGCA GAAGGGCCTG GAAATAAGCA TCGCCGATGA GTCAATGGAG 
CTGGCCCACG CCACAGCCTG CATACTGCGC CGAGGTGTGG TGCGCCTGGC TGCCCTGGTG 
GGCAAGATCT TTGTGAACGA CCAGGAGGAG ACCGTGGTGG ACATCGGCAT GGAGAACGCC 
GTGGCCGGCA AGGTGAAACT CCGCTTTGGC AATGTGGAGG CCAGGCTAGA GTTTGGAGAA 
GATCACGATG AGGTCCACGA CAGTAGCGGA TTCGGGGAAT GCCTGAAGAA CGGATTAAAC 
AACACAACCT TGGACAGCAT GGATGTTCCG GAAACACAGC CTCCATCCGC CAACACATCA 
GTCAACACAA CGGCAGATAG CTTATTTATA CCCGAGACCC AAGCGGTGTT ATGCGAAAGG 
CCTTCGACCG GACAAAGAGT GTCGCTGGGC GATGACTTCA TGATACCCGA AACGCAGGAT 
ATGCTTGCGG ATCTTCCACC TGAACCACCA GTGTTTAAAC CCCCGGTTAT TCCCGTAGTT 
GATAAGCCAG GTCCGACGAT AGACAGCGAT GAGGAATCAT CTCAGGGCAG CATTATACGC 
ATGTGCACCC AGGATTATAA TGAGGATGCC ATCGATGACT TCGATACCTC ACAGGTACTC 
TGCGATGTTC TGCTGCCTCT TCCGCCGCCA ACAGCACCAA TTGAAGGTCT GGAAAATCAG 
GATACTAAGC GGGATCAACT GGACACCACC GACATGGAAA TGAGTGCCTT GAACTGGTCG 
GCTAGCAATT CCAAATGCTG TGCTCTGAGT AGCACCAAAG CGGATGATAT ATTGCCGCGT 
GGCGATGCCT GCATTACTCC CGATTTGACC GCTCCGTCCG TAGATAGAAA TATCTGCACA 
CCCGATCTAT TTGACCTGAT CATGGGGGGG GATAGGAGGC GCGATGGCTC CAGCAGTCCA 
GATCCGTTCG TTCGTCCTGC GGACAATACA AATACCGCCA CACCGCAGTT TGGCGGAGTT 
AAACAGCTGG TTGTGGCGAC GATTGAGACC CCCACTGCCA CTCCTGACAC GAATAGTCAG 
GAAAAGAATC AAGATATGAT AGCCACTCAA CGATTTCCAC GACATAAATT GTTGGAAAGT 
GACGAGGAAG ACAATGGGCA AAACAACCAA GACTTCGTGG CTACTCAGGC GTTTAACTTA 
GGACGTCCGC AGGCACCACA AGAAAATGAA GCCAATCAAG ATCTCATAGC GACACAGGCA 
TTTAACTTGG CACCGCCACA AAAAGTTGAT TCACCTAAAC CCTCTACAGC TAATGATGCT 
TCCAACCAAG ATTTTATAGA AACTCAGGCA TTTAACTTAG GAATCCCACA AGCAAACATG 
GCTTCACCAG AAAATAATGA GATTTCCAAC CAAGATTTGA TAGCCACTCA AGCATTTCCC 
GCCAAGATCA ATAGCTCTCG TTGCCAGGAC TTCGTAGCCA CGCAACCTTT TCATGTGGTC 
AATCAGCATT CCGTGAGTTT ACAGGACAAG GAGAACATTC CACTTGACGA CAGTGTCAAA 
GTTGCATCAG ATTCGAAATT CTTTGAAGCG ACTTGCGGTG AGATAGATGC TGTGCTCAAT 
GAAATGATTT CAGGGACTGT GGCAACGTCG CCTGTAAGTC CCAATGAGGA GTCGATACCA 
TTTTTTGAGC CGTGTGTGAT CAAGGATAAG AATCACTATC AGAAGATCTG TCAGATTGAA 
GGCGTTTTTA GTAACGTGAG TAACAGGAGT CGTGGGCGTA CTGATGGAAC TGGCTTTGGC 
CGTCTCAAAC GGGTGGCCAA ATCGGAATCT CCTCCAGAAA CCCCAATCCG TAAGGACAGA 
CGAAGATCGG AGGGTTCTGA GAGACCGGGA AGTAACTCGA GAGAACGGCT AAACAATTTA 
GTGGATAAAA AATCGGAAGC CCTTAACAAG TCGGTTATCC GGGAACATGG CATCGATTCC 
ATTCCAGAAG AGATAACAAA GGAAGACAAA TCAGACTCAG AAGAGAAAGG CGATTGTTTC 
AACAATGTTG TTAATCAATG GCGCTCCCGT AAAAGACGAA TTCAAAGCCG CCCTGGTACT 
CCAGGAAGCT CTTCAGAAAG GAATGAAGTC AACGCAGATG TGGTGGATAA ACCCAACGAA 
GAGCCACCTA CCAAAAATAT ACGTGCTAGA AAGGCCAAGA AGGCGGAAAA GGCAGACTCA 
TCCAAGGATA TTCCCAAGCC TACGACAAGA ACCAGGCGCC AAACCAGCGA TGAAGATGTT 
CGAACGCCTG ATCTAGGAAA AGGAAGAAAG GCCAAGAACA CTGCTTCGAG TAAGGCAGAC 
ACATCTAAGA ATATTGCCAA AGGCTCGAAG AGAGTCACTC GACAAAATAG CGCTGATGAT 
GCCGAGTTGC TTGAGGAGGA CAGTTCTGTC CAGACAGAAA GCGGAAAAAG GGTTAAGAAG 
GTTAAGTCAA GTAAAGGGGA AACGTCAAAG GACATTCCCA AGGCAAAACC CAGAACTAGG 
CGCCAAACTG CCGACGAAGA TGCTATGACC CCAAAGGCAG AAAAAGAAAG ACAGATTCAG 
GGGGAAGAAA CCAAGGATAT GTCCAAGCCA GCGGCCAGAA CCAGGCGAAA AGCTAGCGCT 
GAAGAGACTG AGAGTGCAGC ACAAGCCGTC AAACCGAAAA GAGGTGGAAA AGGCAAGAAA 
AACGAGACTA CCAAAGCTGA GGCAACTGTG GAAAAGCCTA TGGTTAGAAC CAGGCGCATG 
ACAATCGCCG AAGATTCCAG CACCCTGACG GAAGTAAAAA CAGGGGGCGC ATTAATTAAA 
CGTGCGGTTG TCCGCATATC CAGGGTAAGC ATAGAACAAC TCGAGTCTTC CACAAGCGCT 
GGAAATAATG CCAGAGTAAC TGGGGCTGCT CAGTCGCGTT CTACAACGCC CTCAATTGAA 
GAGCCATCAT CTAGCTCAGC TGCTGCCAAG GAGCCAAATG TATCTACAGC AACTACACGC 
AGGCGAGCTA AGCGCTTAAC ATCTGGCGAA GAGCCCACGT CCAGCTCGGA CGTCCCTACC 
AAGAAGCCTA AAATGTCTGT TGACGAGGAA GTTCTGAAGA ACAGATCAGG CGCTACTACA 
ACGCGATTCA GTCAGCCGGA TGCCGATCCC CTTAGAGCAA TTAACTTGTA CGTGAGAAAG 
GCCAAGACTA CTACGGGCAA AATTAAGGTA GCGTTCACCA TGTGCAATCG TCCAGCTTTG 
GAAACTGTCC TCAAGTCACT GAAGCACGTG GTGGAGATCA CCGAAGATCC CTTGCAGTGC 
GACCTGCTTG TGATGGACAA GGGCGAGCGC ACCTATAAGT TCCTAACAGT CATTGCTTCC 
AACAAACCAG TTCTCTCCAC CAATTGGCTG CACTCTGTGA AGAAGACCAG GAGCATCGAC 
ATAAAGGCAG ACCATCTGTT CAGCGACCCA ACGTTCGAGG AGACCTACAA GTTCAAACCC 
TCCTCCGTAC TGGAACACCC TCGCTTGCTG TATGGCTTGC ACTTTATGCT CGGCAAAGAC 
ATAGTGCCAA AGGCCAACGA AATGAAAGTC ATCATTCACA GCGCCGGCGG CAAGGTTCAT 
GCCCAACCGC CTTCGCTGGC CATCAGCGTG GATCTGTACG TGGTCACCAC CAGCAAGGAT 
ACGAAATCTA AGCGACGTCT GAACAACTAT GAAAAGGTGC ACTTCATCAA AACCGAGGCT 
GTTATGCAGG CGCTCGTTCA ACACAACATT GAAATGCTAC AGGAGCACAC GCTCAAACTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523887.2
CDS	27..3869
Translation	MADVSLFFGGLPAILLKADTIYRIGRQKGLEISIADESMELAHATACILRRGVVRLAALVGKIFVNDQEETVVDIGMENAVAGKVKLRFGNVEARLEFGEDHDEVHDSSGFGECLKNGLNNTTLDSMDVPETQPPSANTSVNTTADSLFIPETQAVLCERPSTGQRVSLGDDFMIPETQDMLADLPPEPPVFKPPVIPVVDKPGPTIDSDEESSQGSIIRMCTQDYNEDAIDDFDTSQVLCDVLLPLPPPTAPIEGLENQDTKRDQLDTTDMEMSALNWSASNSKCCALSSTKADDILPRGDACITPDLTAPSVDRNICTPDLFDLIMGGDRRRDGSSSPDPFVRPADNTNTATPQFGGVKQLVVATIETPTATPDTNSQEKNQDMIATQRFPRHKLLESDEEDNGQNNQDFVATQAFNLGRPQAPQENEANQDLIATQAFNLAPPQKVDSPKPSTANDASNQDFIETQAFNLGIPQANMASPENNEISNQDLIATQAFPAKINSSRCQDFVATQPFHVVNQHSVSLQDKENIPLDDSVKVASDSKFFEATCGEIDAVLNEMISGTVATSPVSPNEESIPFFEPCVIKDKNHYQKICQIEGVFSNVSNRSRGRTDGTGFGRLKRVAKSESPPETPIRKDRRRSEGSERPGSNSRERLNNLVDKKSEALNKSVIREHGIDSIPEEITKEDKSDSEEKGDCFNNVVNQWRSRKRRIQSRPGTPGSSSERNEVNADVVDKPNEEPPTKNIRARKAKKAEKADSSKDIPKPTTRTRRQTSDEDVRTPDLGKGRKAKNTASSKADTSKNIAKGSKRVTRQNSADDAELLEEDSSVQTESGKRVKKVKSSKGETSKDIPKAKPRTRRQTADEDAMTPKAEKERQIQGEETKDMSKPAARTRRKASAEETESAAQAVKPKRGGKGKKNETTKAEATVEKPMVRTRRMTIAEDSSTLTEVKTGGALIKRAVVRISRVSIEQLESSTSAGNNARVTGAAQSRSTTPSIEEPSSSSAAAKEPNVSTATTRRRAKRLTSGEEPTSSSDVPTKKPKMSVDEEVLKNRSGATTTRFSQPDADPLRAINLYVRKAKTTTGKIKVAFTMCNRPALETVLKSLKHVVEITEDPLQCDLLVMDKGERTYKFLTVIASNKPVLSTNWLHSVKKTRSIDIKADHLFSDPTFEETYKFKPSSVLEHPRLLYGLHFMLGKDIVPKANEMKVIIHSAGGKVHAQPPSLAISVDLYVVTTSKDTKSKRRLNNYEKVHFIKTEAVMQALVQHNIEMLQEHTLKL

Target ORF information:

RefSeq Version	NM_079163.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mutator 2 (mu2), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079163.4

ORF Insert Sequence:

ATGGCAGATG TAAGCCTGTT TTTTGGCGGT TTGCCGGCCA TTCTTCTGAA AGCGGATACC 
ATTTACCGCA TTGGGAGGCA GAAGGGCCTG GAAATAAGCA TCGCCGATGA GTCAATGGAG 
CTGGCCCACG CCACAGCCTG CATACTGCGC CGAGGTGTGG TGCGCCTGGC TGCCCTGGTG 
GGCAAGATCT TTGTGAACGA CCAGGAGGAG ACCGTGGTGG ACATCGGCAT GGAGAACGCC 
GTGGCCGGCA AGGTGAAACT CCGCTTTGGC AATGTGGAGG CCAGGCTAGA GTTTGGAGAA 
GATCACGATG AGGTCCACGA CAGTAGCGGA TTCGGGGAAT GCCTGAAGAA CGGATTAAAC 
AACACAACCT TGGACAGCAT GGATGTTCCG GAAACACAGC CTCCATCCGC CAACACATCA 
GTCAACACAA CGGCAGATAG CTTATTTATA CCCGAGACCC AAGCGGTGTT ATGCGAAAGG 
CCTTCGACCG GACAAAGAGT GTCGCTGGGC GATGACTTCA TGATACCCGA AACGCAGGAT 
ATGCTTGCGG ATCTTCCACC TGAACCACCA GTGTTTAAAC CCCCGGTTAT TCCCGTAGTT 
GATAAGCCAG GTCCGACGAT AGACAGCGAT GAGGAATCAT CTCAGGGCAG CATTATACGC 
ATGTGCACCC AGGATTATAA TGAGGATGCC ATCGATGACT TCGATACCTC ACAGGTACTC 
TGCGATGTTC TGCTGCCTCT TCCGCCGCCA ACAGCACCAA TTGAAGGTCT GGAAAATCAG 
GATACTAAGC GGGATCAACT GGACACCACC GACATGGAAA TGAGTGCCTT GAACTGGTCG 
GCTAGCAATT CCAAATGCTG TGCTCTGAGT AGCACCAAAG CGGATGATAT ATTGCCGCGT 
GGCGATGCCT GCATTACTCC CGATTTGACC GCTCCGTCCG TAGATAGAAA TATCTGCACA 
CCCGATCTAT TTGACCTGAT CATGGGGGGG GATAGGAGGC GCGATGGCTC CAGCAGTCCA 
GATCCGTTCG TTCGTCCTGC GGACAATACA AATACCGCCA CACCGCAGTT TGGCGGAGTT 
AAACAGCTGG TTGTGGCGAC GATTGAGACC CCCACTGCCA CTCCTGACAC GAATAGTCAG 
GAAAAGAATC AAGATATGAT AGCCACTCAA CGATTTCCAC GACATAAATT GTTGGAAAGT 
GACGAGGAAG ACAATGGGCA AAACAACCAA GACTTCGTGG CTACTCAGGC GTTTAACTTA 
GGACGTCCGC AGGCACCACA AGAAAATGAA GCCAATCAAG ATCTCATAGC GACACAGGCA 
TTTAACTTGG CACCGCCACA AAAAGTTGAT TCACCTAAAC CCTCTACAGC TAATGATGCT 
TCCAACCAAG ATTTTATAGA AACTCAGGCA TTTAACTTAG GAATCCCACA AGCAAACATG 
GCTTCACCAG AAAATAATGA GATTTCCAAC CAAGATTTGA TAGCCACTCA AGCATTTCCC 
GCCAAGATCA ATAGCTCTCG TTGCCAGGAC TTCGTAGCCA CGCAACCTTT TCATGTGGTC 
AATCAGCATT CCGTGAGTTT ACAGGACAAG GAGAACATTC CACTTGACGA CAGTGTCAAA 
GTTGCATCAG ATTCGAAATT CTTTGAAGCG ACTTGCGGTG AGATAGATGC TGTGCTCAAT 
GAAATGATTT CAGGGACTGT GGCAACGTCG CCTGTAAGTC CCAATGAGGA GTCGATACCA 
TTTTTTGAGC CGTGTGTGAT CAAGGATAAG AATCACTATC AGAAGATCTG TCAGATTGAA 
GGCGTTTTTA GTAACGTGAG TAACAGGAGT CGTGGGCGTA CTGATGGAAC TGGCTTTGGC 
CGTCTCAAAC GGGTGGCCAA ATCGGAATCT CCTCCAGAAA CCCCAATCCG TAAGGACAGA 
CGAAGATCGG AGGGTTCTGA GAGACCGGGA AGTAACTCGA GAGAACGGCT AAACAATTTA 
GTGGATAAAA AATCGGAAGC CCTTAACAAG TCGGTTATCC GGGAACATGG CATCGATTCC 
ATTCCAGAAG AGATAACAAA GGAAGACAAA TCAGACTCAG AAGAGAAAGG CGATTGTTTC 
AACAATGTTG TTAATCAATG GCGCTCCCGT AAAAGACGAA TTCAAAGCCG CCCTGGTACT 
CCAGGAAGCT CTTCAGAAAG GAATGAAGTC AACGCAGATG TGGTGGATAA ACCCAACGAA 
GAGCCACCTA CCAAAAATAT ACGTGCTAGA AAGGCCAAGA AGGCGGAAAA GGCAGACTCA 
TCCAAGGATA TTCCCAAGCC TACGACAAGA ACCAGGCGCC AAACCAGCGA TGAAGATGTT 
CGAACGCCTG ATCTAGGAAA AGGAAGAAAG GCCAAGAACA CTGCTTCGAG TAAGGCAGAC 
ACATCTAAGA ATATTGCCAA AGGCTCGAAG AGAGTCACTC GACAAAATAG CGCTGATGAT 
GCCGAGTTGC TTGAGGAGGA CAGTTCTGTC CAGACAGAAA GCGGAAAAAG GGTTAAGAAG 
GTTAAGTCAA GTAAAGGGGA AACGTCAAAG GACATTCCCA AGGCAAAACC CAGAACTAGG 
CGCCAAACTG CCGACGAAGA TGCTATGACC CCAAAGGCAG AAAAAGAAAG ACAGATTCAG 
GGGGAAGAAA CCAAGGATAT GTCCAAGCCA GCGGCCAGAA CCAGGCGAAA AGCTAGCGCT 
GAAGAGACTG AGAGTGCAGC ACAAGCCGTC AAACCGAAAA GAGGTGGAAA AGGCAAGAAA 
AACGAGACTA CCAAAGCTGA GGCAACTGTG GAAAAGCCTA TGGTTAGAAC CAGGCGCATG 
ACAATCGCCG AAGATTCCAG CACCCTGACG GAAGTAAAAA CAGGGGGCGC ATTAATTAAA 
CGTGCGGTTG TCCGCATATC CAGGGTAAGC ATAGAACAAC TCGAGTCTTC CACAAGCGCT 
GGAAATAATG CCAGAGTAAC TGGGGCTGCT CAGTCGCGTT CTACAACGCC CTCAATTGAA 
GAGCCATCAT CTAGCTCAGC TGCTGCCAAG GAGCCAAATG TATCTACAGC AACTACACGC 
AGGCGAGCTA AGCGCTTAAC ATCTGGCGAA GAGCCCACGT CCAGCTCGGA CGTCCCTACC 
AAGAAGCCTA AAATGTCTGT TGACGAGGAA GTTCTGAAGA ACAGATCAGG CGCTACTACA 
ACGCGATTCA GTCAGCCGGA TGCCGATCCC CTTAGAGCAA TTAACTTGTA CGTGAGAAAG 
GCCAAGACTA CTACGGGCAA AATTAAGGTA GCGTTCACCA TGTGCAATCG TCCAGCTTTG 
GAAACTGTCC TCAAGTCACT GAAGCACGTG GTGGAGATCA CCGAAGATCC CTTGCAGTGC 
GACCTGCTTG TGATGGACAA GGGCGAGCGC ACCTATAAGT TCCTAACAGT CATTGCTTCC 
AACAAACCAG TTCTCTCCAC CAATTGGCTG CACTCTGTGA AGAAGACCAG GAGCATCGAC 
ATAAAGGCAG ACCATCTGTT CAGCGACCCA ACGTTCGAGG AGACCTACAA GTTCAAACCC 
TCCTCCGTAC TGGAACACCC TCGCTTGCTG TATGGCTTGC ACTTTATGCT CGGCAAAGAC 
ATAGTGCCAA AGGCCAACGA AATGAAAGTC ATCATTCACA GCGCCGGCGG CAAGGTTCAT 
GCCCAACCGC CTTCGCTGGC CATCAGCGTG GATCTGTACG TGGTCACCAC CAGCAAGGAT 
ACGAAATCTA AGCGACGTCT GAACAACTAT GAAAAGGTGC ACTTCATCAA AACCGAGGCT 
GTTATGCAGG CGCTCGTTCA ACACAACATT GAAATGCTAC AGGAGCACAC GCTCAAACTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mu2 ( NM_001169855.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163326.1 Drosophila melanogaster mutator 2 (mu2), transcript variant C, mRNA.

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CloneID	OFa20446
Clone ID Related Accession (Same CDS sequence)	NM_001169855.2
Accession Version	NM_001169855.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mutator 2, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001169855.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGATG TAAGCCTGTT TTTTGGCGGT TTGCCGGCCA TTCTTCTGAA AGCGGATACC 
ATTTACCGCA TTGGGAGGCA GAAGGGCCTG GAAATAAGCA TCGCCGATGA GTCAATGGAG 
CTGGCCCACG CCACAGCCTG CATACTGCGC CGAGGTGTGG TGCGCCTGGC TGCCCTGGTG 
GGCAAGATCT TTGTGAACGA CCAGGAGGAG ACCGTGGTGG ACATCGGCAT GGAGAACGCC 
GTGGCCGGCA AGGTGAAACT CCGCTTTGGC AATGTGGAGG CCAGGCTAGA GTTTGGAGAA 
GATCACGATG AGGTCCACGA CAGTAGCGGA TTCGGGGAAT GCCTGAAGAA CGGATTAAAC 
AACACAACCT TGGACAGCAT GGATGTTCCG GAAACACAGC CTCCATCCGC CAACACATCA 
GTCAACACAA CGGCAGATAG CTTATTTATA CCCGAGACCC AAGCGGTGTT ATGCGAAAGG 
CCTTCGACCG GACAAAGAGT GTCGCTGGGC GATGACTTCA TGATACCCGA AACGCAGGAT 
ATGCTTGCGG ATCTTCCACC TGAACCACCA GTGTTTAAAC CCCCGGTTAT TCCCGTAGTT 
GATAAGCCAG GTCCGACGAT AGACAGCGAT GAGGAATCAT CTCAGGGCAG CATTATACGC 
ATGTGCACCC AGGATTATAA TGAGGATGCC ATCGATGACT TCGATACCTC ACAGGTACTC 
TGCGATGTTC TGCTGCCTCT TCCGCCGCCA ACAGCACCAA TTGAAGGTCT GGAAAATCAG 
GATACTAAGC GGGATCAACT GGACACCACC GACATGGAAA TGAGTGCCTT GAACTGGTCG 
GCTAGCAATT CCAAATGCTG TGCTCTGAGT AGCACCAAAG CGGATGATAT ATTGCCGCGT 
GGCGATGCCT GCATTACTCC CGATTTGACC GCTCCGTCCG TAGATAGAAA TATCTGCACA 
CCCGATCTAT TTGACCTGAT CATGGGGGGG GATAGGAGGC GCGATGGCTC CAGCAGTCCA 
GATCCGTTCG TTCGTCCTGC GGACAATACA AATACCGCCA CACCGCAGTT TGGCGGAGTT 
AAACAGCTGG TTGTGGCGAC GATTGAGACC CCCACTGCCA CTCCTGACAC GAATAGTCAG 
GAAAAGAATC AAGATATGAT AGCCACTCAA CGATTTCCAC GACATAAATT GTTGGAAAGT 
GACGAGGAAG ACAATGGGCA AAACAACCAA GACTTCGTGG CTACTCAGGC GTTTAACTTA 
GGACGTCCGC AGGCACCACA AGAAAATGAA GCCAATCAAG ATCTCATAGC GACACAGGCA 
TTTAACTTGG CACCGCCACA AAAAGTTGAT TCACCTAAAC CCTCTACAGC TAATGATGCT 
TCCAACCAAG ATTTTATAGA AACTCAGGCA TTTAACTTAG GAATCCCACA AGCAAACATG 
GCTTCACCAG AAAATAATGA GATTTCCAAC CAAGATTTGA TAGCCACTCA AGCATTTCCC 
GCCAAGATCA ATAGCTCTCG TTGCCAGGAC TTCGTAGCCA CGCAACCTTT TCATGTGGTC 
AATCAGCATT CCGTGAGTTT ACAGGACAAG GAGAACATTC CACTTGACGA CAGTGTCAAA 
GTTGCATCAG CGACTTGCGG TGAGATAGAT GCTGTGCTCA ATGAAATGAT TTCAGGGACT 
GTGGCAACGT CGCCTGTAAG TCCCAATGAG GAGTCGATAC CATTTTTTGA GCCGTGTGTG 
ATCAAGGATA AGAATCACTA TCAGAAGATC TGTCAGATTG AAGGCGTTTT TAGTAACGTG 
AGTAACAGGA GTCGTGGGCG TACTGATGGA ACTGGCTTTG GCCGTCTCAA ACGGGTGGCC 
AAATCGGAAT CTCCTCCAGA AACCCCAATC CGTAAGGACA GACGAAGATC GGAGGGTTCT 
GAGAGACCGG GAAGTAACTC GAGAGAACGG CTAAACAATT TAGTGGATAA AAAATCGGAA 
GCCCTTAACA AGTCGGTTAT CCGGGAACAT GGCATCGATT CCATTCCAGA AGAGATAACA 
AAGGAAGACA AATCAGACTC AGAAGAGAAA GGCGATTGTT TCAACAATGT TGTTAATCAA 
TGGCGCTCCC GTAAAAGACG AATTCAAAGC CGCCCTGGTA CTCCAGGAAG CTCTTCAGAA 
AGGAATGAAG TCAACGCAGA TGTGGTGGAT AAACCCAACG AAGAGCCACC TACCAAAAAT 
ATACGTGCTA GAAAGGCCAA GAAGGCGGAA AAGGCAGACT CATCCAAGGA TATTCCCAAG 
CCTACGACAA GAACCAGGCG CCAAACCAGC GATGAAGATG TTCGAACGCC TGATCTAGGA 
AAAGGAAGAA AGGCCAAGAA CACTGCTTCG AGTAAGGCAG ACACATCTAA GAATATTGCC 
AAAGGCTCGA AGAGAGTCAC TCGACAAAAT AGCGCTGATG ATGCCGAGTT GCTTGAGGAG 
GACAGTTCTG TCCAGACAGA AAGCGGAAAA AGGGTTAAGA AGGTTAAGTC AAGTAAAGGG 
GAAACGTCAA AGGACATTCC CAAGGCAAAA CCCAGAACTA GGCGCCAAAC TGCCGACGAA 
GATGCTATGA CCCCAAAGGC AGAAAAAGAA AGACAGATTC AGGGGGAAGA AACCAAGGAT 
ATGTCCAAGC CAGCGGCCAG AACCAGGCGA AAAGCTAGCG CTGAAGAGAC TGAGAGTGCA 
GCACAAGCCG TCAAACCGAA AAGAGGTGGA AAAGGCAAGA AAAACGAGAC TACCAAAGCT 
GAGGCAACTG TGGAAAAGCC TATGGTTAGA ACCAGGCGCA TGACAATCGC CGAAGATTCC 
AGCACCCTGA CGGAAGTAAA AACAGGGGGC GCATTAATTA AACGTGCGGT TGTCCGCATA 
TCCAGGGTAA GCATAGAACA ACTCGAGTCT TCCACAAGCG CTGGAAATAA TGCCAGAGTA 
ACTGGGGCTG CTCAGTCGCG TTCTACAACG CCCTCAATTG AAGAGCCATC ATCTAGCTCA 
GCTGCTGCCA AGGAGCCAAA TGTATCTACA GCAACTACAC GCAGGCGAGC TAAGCGCTTA 
ACATCTGGCG AAGAGCCCAC GTCCAGCTCG GACGTCCCTA CCAAGAAGCC TAAAATGTCT 
GTTGACGAGG AAGTTCTGAA GAACAGATCA GGCGCTACTA CAACGCGATT CAGTCAGCCG 
GATGCCGATC CCCTTAGAGC AATTAACTTG TACGTGAGAA AGGCCAAGAC TACTACGGGC 
AAAATTAAGG TAGCGTTCAC CATGTGCAAT CGTCCAGCTT TGGAAACTGT CCTCAAGTCA 
CTGAAGCACG TGGTGGAGAT CACCGAAGAT CCCTTGCAGT GCGACCTGCT TGTGATGGAC 
AAGGGCGAGC GCACCTATAA GTTCCTAACA GTCATTGCTT CCAACAAACC AGTTCTCTCC 
ACCAATTGGC TGCACTCTGT GAAGAAGACC AGGAGCATCG ACATAAAGGC AGACCATCTG 
TTCAGCGACC CAACGTTCGA GGAGACCTAC AAGTTCAAAC CCTCCTCCGT ACTGGAACAC 
CCTCGCTTGC TGTATGGCTT GCACTTTATG CTCGGCAAAG ACATAGTGCC AAAGGCCAAC 
GAAATGAAAG TCATCATTCA CAGCGCCGGC GGCAAGGTTC ATGCCCAACC GCCTTCGCTG 
GCCATCAGCG TGGATCTGTA CGTGGTCACC ACCAGCAAGG ATACGAAATC TAAGCGACGT 
CTGAACAACT ATGAAAAGGT GCACTTCATC AAAACCGAGG CTGTTATGCA GGCGCTCGTT 
CAACACAACA TTGAAATGCT ACAGGAGCAC ACGCTCAAAC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163326.1
CDS	27..3851
Translation	MADVSLFFGGLPAILLKADTIYRIGRQKGLEISIADESMELAHATACILRRGVVRLAALVGKIFVNDQEETVVDIGMENAVAGKVKLRFGNVEARLEFGEDHDEVHDSSGFGECLKNGLNNTTLDSMDVPETQPPSANTSVNTTADSLFIPETQAVLCERPSTGQRVSLGDDFMIPETQDMLADLPPEPPVFKPPVIPVVDKPGPTIDSDEESSQGSIIRMCTQDYNEDAIDDFDTSQVLCDVLLPLPPPTAPIEGLENQDTKRDQLDTTDMEMSALNWSASNSKCCALSSTKADDILPRGDACITPDLTAPSVDRNICTPDLFDLIMGGDRRRDGSSSPDPFVRPADNTNTATPQFGGVKQLVVATIETPTATPDTNSQEKNQDMIATQRFPRHKLLESDEEDNGQNNQDFVATQAFNLGRPQAPQENEANQDLIATQAFNLAPPQKVDSPKPSTANDASNQDFIETQAFNLGIPQANMASPENNEISNQDLIATQAFPAKINSSRCQDFVATQPFHVVNQHSVSLQDKENIPLDDSVKVASATCGEIDAVLNEMISGTVATSPVSPNEESIPFFEPCVIKDKNHYQKICQIEGVFSNVSNRSRGRTDGTGFGRLKRVAKSESPPETPIRKDRRRSEGSERPGSNSRERLNNLVDKKSEALNKSVIREHGIDSIPEEITKEDKSDSEEKGDCFNNVVNQWRSRKRRIQSRPGTPGSSSERNEVNADVVDKPNEEPPTKNIRARKAKKAEKADSSKDIPKPTTRTRRQTSDEDVRTPDLGKGRKAKNTASSKADTSKNIAKGSKRVTRQNSADDAELLEEDSSVQTESGKRVKKVKSSKGETSKDIPKAKPRTRRQTADEDAMTPKAEKERQIQGEETKDMSKPAARTRRKASAEETESAAQAVKPKRGGKGKKNETTKAEATVEKPMVRTRRMTIAEDSSTLTEVKTGGALIKRAVVRISRVSIEQLESSTSAGNNARVTGAAQSRSTTPSIEEPSSSSAAAKEPNVSTATTRRRAKRLTSGEEPTSSSDVPTKKPKMSVDEEVLKNRSGATTTRFSQPDADPLRAINLYVRKAKTTTGKIKVAFTMCNRPALETVLKSLKHVVEITEDPLQCDLLVMDKGERTYKFLTVIASNKPVLSTNWLHSVKKTRSIDIKADHLFSDPTFEETYKFKPSSVLEHPRLLYGLHFMLGKDIVPKANEMKVIIHSAGGKVHAQPPSLAISVDLYVVTTSKDTKSKRRLNNYEKVHFIKTEAVMQALVQHNIEMLQEHTLKL

Target ORF information:

RefSeq Version	NM_001169855.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mutator 2 (mu2), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169855.2

ORF Insert Sequence:

ATGGCAGATG TAAGCCTGTT TTTTGGCGGT TTGCCGGCCA TTCTTCTGAA AGCGGATACC 
ATTTACCGCA TTGGGAGGCA GAAGGGCCTG GAAATAAGCA TCGCCGATGA GTCAATGGAG 
CTGGCCCACG CCACAGCCTG CATACTGCGC CGAGGTGTGG TGCGCCTGGC TGCCCTGGTG 
GGCAAGATCT TTGTGAACGA CCAGGAGGAG ACCGTGGTGG ACATCGGCAT GGAGAACGCC 
GTGGCCGGCA AGGTGAAACT CCGCTTTGGC AATGTGGAGG CCAGGCTAGA GTTTGGAGAA 
GATCACGATG AGGTCCACGA CAGTAGCGGA TTCGGGGAAT GCCTGAAGAA CGGATTAAAC 
AACACAACCT TGGACAGCAT GGATGTTCCG GAAACACAGC CTCCATCCGC CAACACATCA 
GTCAACACAA CGGCAGATAG CTTATTTATA CCCGAGACCC AAGCGGTGTT ATGCGAAAGG 
CCTTCGACCG GACAAAGAGT GTCGCTGGGC GATGACTTCA TGATACCCGA AACGCAGGAT 
ATGCTTGCGG ATCTTCCACC TGAACCACCA GTGTTTAAAC CCCCGGTTAT TCCCGTAGTT 
GATAAGCCAG GTCCGACGAT AGACAGCGAT GAGGAATCAT CTCAGGGCAG CATTATACGC 
ATGTGCACCC AGGATTATAA TGAGGATGCC ATCGATGACT TCGATACCTC ACAGGTACTC 
TGCGATGTTC TGCTGCCTCT TCCGCCGCCA ACAGCACCAA TTGAAGGTCT GGAAAATCAG 
GATACTAAGC GGGATCAACT GGACACCACC GACATGGAAA TGAGTGCCTT GAACTGGTCG 
GCTAGCAATT CCAAATGCTG TGCTCTGAGT AGCACCAAAG CGGATGATAT ATTGCCGCGT 
GGCGATGCCT GCATTACTCC CGATTTGACC GCTCCGTCCG TAGATAGAAA TATCTGCACA 
CCCGATCTAT TTGACCTGAT CATGGGGGGG GATAGGAGGC GCGATGGCTC CAGCAGTCCA 
GATCCGTTCG TTCGTCCTGC GGACAATACA AATACCGCCA CACCGCAGTT TGGCGGAGTT 
AAACAGCTGG TTGTGGCGAC GATTGAGACC CCCACTGCCA CTCCTGACAC GAATAGTCAG 
GAAAAGAATC AAGATATGAT AGCCACTCAA CGATTTCCAC GACATAAATT GTTGGAAAGT 
GACGAGGAAG ACAATGGGCA AAACAACCAA GACTTCGTGG CTACTCAGGC GTTTAACTTA 
GGACGTCCGC AGGCACCACA AGAAAATGAA GCCAATCAAG ATCTCATAGC GACACAGGCA 
TTTAACTTGG CACCGCCACA AAAAGTTGAT TCACCTAAAC CCTCTACAGC TAATGATGCT 
TCCAACCAAG ATTTTATAGA AACTCAGGCA TTTAACTTAG GAATCCCACA AGCAAACATG 
GCTTCACCAG AAAATAATGA GATTTCCAAC CAAGATTTGA TAGCCACTCA AGCATTTCCC 
GCCAAGATCA ATAGCTCTCG TTGCCAGGAC TTCGTAGCCA CGCAACCTTT TCATGTGGTC 
AATCAGCATT CCGTGAGTTT ACAGGACAAG GAGAACATTC CACTTGACGA CAGTGTCAAA 
GTTGCATCAG CGACTTGCGG TGAGATAGAT GCTGTGCTCA ATGAAATGAT TTCAGGGACT 
GTGGCAACGT CGCCTGTAAG TCCCAATGAG GAGTCGATAC CATTTTTTGA GCCGTGTGTG 
ATCAAGGATA AGAATCACTA TCAGAAGATC TGTCAGATTG AAGGCGTTTT TAGTAACGTG 
AGTAACAGGA GTCGTGGGCG TACTGATGGA ACTGGCTTTG GCCGTCTCAA ACGGGTGGCC 
AAATCGGAAT CTCCTCCAGA AACCCCAATC CGTAAGGACA GACGAAGATC GGAGGGTTCT 
GAGAGACCGG GAAGTAACTC GAGAGAACGG CTAAACAATT TAGTGGATAA AAAATCGGAA 
GCCCTTAACA AGTCGGTTAT CCGGGAACAT GGCATCGATT CCATTCCAGA AGAGATAACA 
AAGGAAGACA AATCAGACTC AGAAGAGAAA GGCGATTGTT TCAACAATGT TGTTAATCAA 
TGGCGCTCCC GTAAAAGACG AATTCAAAGC CGCCCTGGTA CTCCAGGAAG CTCTTCAGAA 
AGGAATGAAG TCAACGCAGA TGTGGTGGAT AAACCCAACG AAGAGCCACC TACCAAAAAT 
ATACGTGCTA GAAAGGCCAA GAAGGCGGAA AAGGCAGACT CATCCAAGGA TATTCCCAAG 
CCTACGACAA GAACCAGGCG CCAAACCAGC GATGAAGATG TTCGAACGCC TGATCTAGGA 
AAAGGAAGAA AGGCCAAGAA CACTGCTTCG AGTAAGGCAG ACACATCTAA GAATATTGCC 
AAAGGCTCGA AGAGAGTCAC TCGACAAAAT AGCGCTGATG ATGCCGAGTT GCTTGAGGAG 
GACAGTTCTG TCCAGACAGA AAGCGGAAAA AGGGTTAAGA AGGTTAAGTC AAGTAAAGGG 
GAAACGTCAA AGGACATTCC CAAGGCAAAA CCCAGAACTA GGCGCCAAAC TGCCGACGAA 
GATGCTATGA CCCCAAAGGC AGAAAAAGAA AGACAGATTC AGGGGGAAGA AACCAAGGAT 
ATGTCCAAGC CAGCGGCCAG AACCAGGCGA AAAGCTAGCG CTGAAGAGAC TGAGAGTGCA 
GCACAAGCCG TCAAACCGAA AAGAGGTGGA AAAGGCAAGA AAAACGAGAC TACCAAAGCT 
GAGGCAACTG TGGAAAAGCC TATGGTTAGA ACCAGGCGCA TGACAATCGC CGAAGATTCC 
AGCACCCTGA CGGAAGTAAA AACAGGGGGC GCATTAATTA AACGTGCGGT TGTCCGCATA 
TCCAGGGTAA GCATAGAACA ACTCGAGTCT TCCACAAGCG CTGGAAATAA TGCCAGAGTA 
ACTGGGGCTG CTCAGTCGCG TTCTACAACG CCCTCAATTG AAGAGCCATC ATCTAGCTCA 
GCTGCTGCCA AGGAGCCAAA TGTATCTACA GCAACTACAC GCAGGCGAGC TAAGCGCTTA 
ACATCTGGCG AAGAGCCCAC GTCCAGCTCG GACGTCCCTA CCAAGAAGCC TAAAATGTCT 
GTTGACGAGG AAGTTCTGAA GAACAGATCA GGCGCTACTA CAACGCGATT CAGTCAGCCG 
GATGCCGATC CCCTTAGAGC AATTAACTTG TACGTGAGAA AGGCCAAGAC TACTACGGGC 
AAAATTAAGG TAGCGTTCAC CATGTGCAAT CGTCCAGCTT TGGAAACTGT CCTCAAGTCA 
CTGAAGCACG TGGTGGAGAT CACCGAAGAT CCCTTGCAGT GCGACCTGCT TGTGATGGAC 
AAGGGCGAGC GCACCTATAA GTTCCTAACA GTCATTGCTT CCAACAAACC AGTTCTCTCC 
ACCAATTGGC TGCACTCTGT GAAGAAGACC AGGAGCATCG ACATAAAGGC AGACCATCTG 
TTCAGCGACC CAACGTTCGA GGAGACCTAC AAGTTCAAAC CCTCCTCCGT ACTGGAACAC 
CCTCGCTTGC TGTATGGCTT GCACTTTATG CTCGGCAAAG ACATAGTGCC AAAGGCCAAC 
GAAATGAAAG TCATCATTCA CAGCGCCGGC GGCAAGGTTC ATGCCCAACC GCCTTCGCTG 
GCCATCAGCG TGGATCTGTA CGTGGTCACC ACCAGCAAGG ATACGAAATC TAAGCGACGT 
CTGAACAACT ATGAAAAGGT GCACTTCATC AAAACCGAGG CTGTTATGCA GGCGCTCGTT 
CAACACAACA TTGAAATGCT ACAGGAGCAC ACGCTCAAAC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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mu2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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