dre4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	dre4
Entrez Gene ID	38248
Full Name	CG1828 gene product from transcript CG1828-RB
Synonyms	CG1828,DRE4,DRE4/dSPT16,Dmel\CG1828,Dre4,SPT16,Spt16,dSPT16,dSpt16,dspt16,l(3)62Bg,l(3)dre4,spt16
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 62B7-62B7

mRNA and Protein(s)

mRNA	Protein	Name
NM_167922.3	NP_728686.2	dre4, isoform B
NM_057262.4	NP_476610.2	dre4, isoform A

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Supt16	NP_291096.2
Kluyveromyces lactis	KLLA0F09889g	XP_455530.1
Eremothecium gossypii	AGOS_AER360C	NP_985216.2
Schizosaccharomyces pombe (fission yeast)	spt16	NP_596526.1
Oryza sativa (rice)	Os04g0321600	NP_001052463.1
Pan troglodytes (chimpanzee)	SUPT16H	XP_528703.2
Rattus norvegicus (Norway rat)	Supt16h	NP_001100731.1
Danio rerio (zebrafish)	supt16h	NP_001091053.1
Bos taurus (cattle)	SUPT16H	NP_001179321.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006817	XP_308929.4
Neurospora crassa	NCU01164	XP_961530.2
Arabidopsis thaliana (thale cress)	SPT16	NP_192809.1
Homo sapiens (human)	SUPT16H	NP_009123.1
Canis lupus familiaris (dog)	SUPT16H	XP_851566.1
Saccharomyces cerevisiae (baker's yeast)	SPT16	NP_011308.1
Drosophila melanogaster (fruit fly)	dre4	NP_728686.2
Caenorhabditis elegans (roundworm)	F55A3.3	NP_492821.1
Magnaporthe oryzae (rice blast fungus)	MGG_07316	XP_003715548.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results show that FACT, a complex that comprises Spt16 and SSRP1, is associated with actively transcribed RNA polymerase II genes on Drosophila polytene chromosomes
Title: Tracking FACT and the RNA polymerase II elongation complex through chromatin in vivo.

results show that FACT, a heterodimer of dSPT16 and dSSRP1, associates with GAGA factor through its dSSRP1 subunit & facilitates chromatin remodeling; interactions between GAGA factor and spt16 implicate the GAGA factor-FACT complex in Hox gene expression
Title: Drosophila FACT contributes to Hox gene expression through physical and functional interactions with GAGA factor.

The following dre4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dre4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17243	NM_167922.3 Latest version!	Drosophila melanogaster dre4, transcript variant B (dre4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa17244	NM_057262.4 Latest version!	Drosophila melanogaster dre4, transcript variant A (dre4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dre4 ( NM_167922.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728686.2 Drosophila melanogaster dre4, transcript variant B (dre4), mRNA.

Price & Availability↑

CloneID	OFa17243
Clone ID Related Accession (Same CDS sequence)	NM_167922.3
Accession Version	NM_167922.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dre4, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_167922.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGAGCT TTGTGCTGGA CAAGGAGGCG TTCGTGCGCC GCGTGAAACG CCTCTACACG 
GAATGGAGGG CTCCCAGCAT CGGACACGAT GACGCGCTCA GGAATCTGGA CTGCATCATG 
AGCATCGTGG GCGTCGAAGA GGATGTCATG TACTCCAAGT CCATGGCCCT GCAGCTCTGG 
CTGCTGGGCT ACGAGCTGAC GGACACCATC TCAGTGTTTT GCTCGGATGC CGTGTATTTT 
CTCACTAGCA AAAAGAAAAT AGAATTCCTG AAGCAGACGC AGAACATCAC CGAAGAAGGT 
TTTCCGGAAA TCAATCTGCT TGTTAGGGAT CGGACGGACA AGGACCAGGG CAACTTCGAG 
AAGCTCATCA AAGCCCTGCA AAACTCGAAG AAGGGCAAGC GTTTGGGCGT CTTCGCCAAG 
GACGCCTATC CCGGGGAGTT CAGCGAAGCA TGGAAGAAAT CCCTGACGGC GTCCAAGTTC 
GAGCATGTGG ACATTAGCAC TATTATCGCC TACCTCATGT GTCCCAAGGA TGAGTCGGAG 
ATCAACAACA TACGCAAGGC ATCGCTAGTG TCAATGGACA TTTTCAACAA GTATTTGAAA 
GATGAGATCA TGGACATTAT TGATTCGGAT AGGAAAGTCA AACACAACAA GCTCTCAGAT 
GGCTGCGAGG CTGCCATTGG CGAGAAGAAG TACACCAGTG GTCTGGATCC CAGACTCCTC 
GATATGGCCT ACCCACCCAT CATCCAATCG GGAGGAGCCT ACAGCCTGAA GTTCTCCGCG 
GTGGCGGACA AAAATCCCTT GCACTTCGGT GTGATTGTGT GCTCCTTGGG CGCCCGCTAC 
AAGTCGTATT GCTCAAATAT ATCGCGCACC TTTCTGGTGA ATCCCACCGA GGCCATGCAG 
GAGAACTACA CATTTCTGGT AAGTGTGCAG GAGGAGATTC TCAAGCTGCT GGTTCCGGGC 
ACAAAGCTGT GCGACGTCTA CGAAAAGACC CTGGACTTCG TGAAGAAGGA GAAGCCGAGT 
ATGGTGGACA ATCTGCCCAA AAGCTTTGGT TTCGCCATGG GCCTAGAGTT CCGGGAGAAC 
TCCATTGTCA TCGGACCCAA ATGCCAGGCG CTACTCAAGA AGAATATGGT CTTTAATTTG 
CACGTGGGCA TCTCGAATCT GACCAATCCC GAAGCCACCG ACAAAGAGGG CAAGAACTAC 
GCCCTCTTCA TCGGAGACAC TGTGCTAGTA GGCGAACAAT CCCCAGCCAG TGTGATGACT 
CCGTCCAAGA AGAAGATCAA GAACGTCGGC ATCTTTATCA AGGACGACAG CGACGAGGAA 
GATGTGGACG ACAAGAAGAC CGCTAAAGAA GATCAGGGCA CCGAGATCCT GGGCCGCAGC 
AAGCGCAACG CGGTGCTGGA GTCAAAGCTG CGAAACGAGA TCAACACGGA GGAGAAGCGC 
AAGGAGCACC AGCGCGAGCT GGCTCAGCAG CTGAACGAGC GCGCCAAGGA TCGGCTGGCC 
AGGCAGGGAA ACTCGAAGGA GGTCGAGAAA GTGCGCAAGA ACACCGTCTC CTACAAGTCC 
ATCAGCCAAA TGCCGCGCGA GCCGGAAGTC AAGGAGCTCA AGCTTTACGT GGATAAGAAG 
TACGAGACCG TCATCATGCC AGTGTTCGGC ATCCAGGTGC CGTTCCATAT ATCCACCATC 
AAGAACATCT CGCAGTCGGT GGAGGGCGAG TACACGTACT TGCGTATCAA CTTCTTTCAT 
CCCGGCGCCA CAATGGGTCG CAACGAAGGC GGACTCTATC CTCAGCCAGA GGCCACATTT 
GTCAAGGAAG TTACATACCG CAGCTCTAAT GTTAAGGAAC ACGGGGAGGT GGGAGCTCCA 
TCCGCAAATC TAAACAATGC TTTCCGGCTG ATCAAGGAGG TGCAGAAGCG CTTCAAGACC 
CGCGAGGCTG AGGAGCGCGA AAAGGAGGAT CTTGTGAAGC AAGACACACT CATTCTGTCC 
CAGAACAAGG GTAATCCCAA GCTGAAGGAC CTATACATTC GGCCCAACAT CGTGACCAAG 
CGCATGACGG GCAGCCTGGA AGCGCACAGC AATGGATTCC GCTACATCTC CGTGCGCGGC 
GACAAGGTGG ACATCTTGTA CAACAACATC AAAAGCGCGT TTTTCCAGCC CTGCGATGGC 
GAGATGATCA TTCTTCTGCA TTTCCACCTG AAGTACGCCA TTATGTTTGG CAAAAAGAAG 
CACGTGGATG TACAGTTCTA CACGGAAGTG GGAGAGATCA CCACGGATCT GGGCAAGCAC 
CAGCACATGC ACGACCGCGA CGACTTGGCT GCTGAGCAGG CAGAACGTGA GCTGCGGCAC 
AAGCTCAAGA CTGCCTTCAA GAGCTTCTGC GAGAAGGTGG AAACCATGAC CAAGTCGGTA 
GTGGAGTTCG ACACGCCGTT CCGTGAGTTG GGATTCCCTG GAGCACCCTT CCGCAGCACG 
GTGACGCTGC AACCCACCTC GGGCTCCCTG GTCAATCTGA CGGAATGGCC ACCATTTGTC 
ATCACGCTGG ACGATGTGGA GTTGGTGCAC TTCGAGCGCG TTCAGTTCCA CTTGCGCAAC 
TTCGATATGA TATTTGTGTT CAAGGAGTAC AATAAGAAGG TTGCCATGGT GAACGCCATT 
CCTATGAACA TGCTGGATCA CGTGAAGGAG TGGCTCAACT CCTGCGACAT CCGCTATTCG 
GAGGGTGTTC AATCCCTGAA CTGGCAAAAG ATCATGAAGA CCATCACCGA CGATCCTGAG 
GGATTCTTTG AACAAGGCGG CTGGACATTC TTGGACCCGG AATCGGGCAG CGAAGGCGAA 
AACGAAACCG CCGAATCCGA AGAGGACGAG GCCTACAATC CCACCGACGC CGAATCGGAT 
GAGGAGTCGG ACGAAGATTC CGAGTACTCA GAGGCATCAG AAGACAGCGA GGAAAGCGAT 
GAGGACCTTG GCTCTGATGA GGAATCTGGC AAGGATTGGT CCGATCTGGA GCGCGAGGCT 
GCTGAGGAAG ACCGCAACCA CGATTATGCC GCAGATGACA AACCACGCAA CGGCAAATTC 
GATTCGAAGA AGCACGGCAA GAGTTCAAAG CACAGCCCGT CGAAGTCGTC GAAGGACAAG 
TACAACAGCC GGGACAAGCA TCATTCCTCC TCATCGTCCG GAAACAAGAG CAGCAGCAAG 
GACAAGGATC GCAAGCGCTC GCGGGATGAC AGTCGCGACA ATGGGCACAA GTCGAAGAAG 
TCGCGACATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728686.2
CDS	135..3386
Translation	MSSFVLDKEAFVRRVKRLYTEWRAPSIGHDDALRNLDCIMSIVGVEEDVMYSKSMALQLWLLGYELTDTISVFCSDAVYFLTSKKKIEFLKQTQNITEEGFPEINLLVRDRTDKDQGNFEKLIKALQNSKKGKRLGVFAKDAYPGEFSEAWKKSLTASKFEHVDISTIIAYLMCPKDESEINNIRKASLVSMDIFNKYLKDEIMDIIDSDRKVKHNKLSDGCEAAIGEKKYTSGLDPRLLDMAYPPIIQSGGAYSLKFSAVADKNPLHFGVIVCSLGARYKSYCSNISRTFLVNPTEAMQENYTFLVSVQEEILKLLVPGTKLCDVYEKTLDFVKKEKPSMVDNLPKSFGFAMGLEFRENSIVIGPKCQALLKKNMVFNLHVGISNLTNPEATDKEGKNYALFIGDTVLVGEQSPASVMTPSKKKIKNVGIFIKDDSDEEDVDDKKTAKEDQGTEILGRSKRNAVLESKLRNEINTEEKRKEHQRELAQQLNERAKDRLARQGNSKEVEKVRKNTVSYKSISQMPREPEVKELKLYVDKKYETVIMPVFGIQVPFHISTIKNISQSVEGEYTYLRINFFHPGATMGRNEGGLYPQPEATFVKEVTYRSSNVKEHGEVGAPSANLNNAFRLIKEVQKRFKTREAEEREKEDLVKQDTLILSQNKGNPKLKDLYIRPNIVTKRMTGSLEAHSNGFRYISVRGDKVDILYNNIKSAFFQPCDGEMIILLHFHLKYAIMFGKKKHVDVQFYTEVGEITTDLGKHQHMHDRDDLAAEQAERELRHKLKTAFKSFCEKVETMTKSVVEFDTPFRELGFPGAPFRSTVTLQPTSGSLVNLTEWPPFVITLDDVELVHFERVQFHLRNFDMIFVFKEYNKKVAMVNAIPMNMLDHVKEWLNSCDIRYSEGVQSLNWQKIMKTITDDPEGFFEQGGWTFLDPESGSEGENETAESEEDEAYNPTDAESDEESDEDSEYSEASEDSEESDEDLGSDEESGKDWSDLEREAAEEDRNHDYAADDKPRNGKFDSKKHGKSSKHSPSKSSKDKYNSRDKHHSSSSSGNKSSSKDKDRKRSRDDSRDNGHKSKKSRH

Target ORF information:

RefSeq Version	NM_167922.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dre4, transcript variant B (dre4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167922.3

ORF Insert Sequence:

ATGTCGAGCT TTGTGCTGGA CAAGGAGGCG TTCGTGCGCC GCGTGAAACG CCTCTACACG 
GAATGGAGGG CTCCCAGCAT CGGACACGAT GACGCGCTCA GGAATCTGGA CTGCATCATG 
AGCATCGTGG GCGTCGAAGA GGATGTCATG TACTCCAAGT CCATGGCCCT GCAGCTCTGG 
CTGCTGGGCT ACGAGCTGAC GGACACCATC TCAGTGTTTT GCTCGGATGC CGTGTATTTT 
CTCACTAGCA AAAAGAAAAT AGAATTCCTG AAGCAGACGC AGAACATCAC CGAAGAAGGT 
TTTCCGGAAA TCAATCTGCT TGTTAGGGAT CGGACGGACA AGGACCAGGG CAACTTCGAG 
AAGCTCATCA AAGCCCTGCA AAACTCGAAG AAGGGCAAGC GTTTGGGCGT CTTCGCCAAG 
GACGCCTATC CCGGGGAGTT CAGCGAAGCA TGGAAGAAAT CCCTGACGGC GTCCAAGTTC 
GAGCATGTGG ACATTAGCAC TATTATCGCC TACCTCATGT GTCCCAAGGA TGAGTCGGAG 
ATCAACAACA TACGCAAGGC ATCGCTAGTG TCAATGGACA TTTTCAACAA GTATTTGAAA 
GATGAGATCA TGGACATTAT TGATTCGGAT AGGAAAGTCA AACACAACAA GCTCTCAGAT 
GGCTGCGAGG CTGCCATTGG CGAGAAGAAG TACACCAGTG GTCTGGATCC CAGACTCCTC 
GATATGGCCT ACCCACCCAT CATCCAATCG GGAGGAGCCT ACAGCCTGAA GTTCTCCGCG 
GTGGCGGACA AAAATCCCTT GCACTTCGGT GTGATTGTGT GCTCCTTGGG CGCCCGCTAC 
AAGTCGTATT GCTCAAATAT ATCGCGCACC TTTCTGGTGA ATCCCACCGA GGCCATGCAG 
GAGAACTACA CATTTCTGGT AAGTGTGCAG GAGGAGATTC TCAAGCTGCT GGTTCCGGGC 
ACAAAGCTGT GCGACGTCTA CGAAAAGACC CTGGACTTCG TGAAGAAGGA GAAGCCGAGT 
ATGGTGGACA ATCTGCCCAA AAGCTTTGGT TTCGCCATGG GCCTAGAGTT CCGGGAGAAC 
TCCATTGTCA TCGGACCCAA ATGCCAGGCG CTACTCAAGA AGAATATGGT CTTTAATTTG 
CACGTGGGCA TCTCGAATCT GACCAATCCC GAAGCCACCG ACAAAGAGGG CAAGAACTAC 
GCCCTCTTCA TCGGAGACAC TGTGCTAGTA GGCGAACAAT CCCCAGCCAG TGTGATGACT 
CCGTCCAAGA AGAAGATCAA GAACGTCGGC ATCTTTATCA AGGACGACAG CGACGAGGAA 
GATGTGGACG ACAAGAAGAC CGCTAAAGAA GATCAGGGCA CCGAGATCCT GGGCCGCAGC 
AAGCGCAACG CGGTGCTGGA GTCAAAGCTG CGAAACGAGA TCAACACGGA GGAGAAGCGC 
AAGGAGCACC AGCGCGAGCT GGCTCAGCAG CTGAACGAGC GCGCCAAGGA TCGGCTGGCC 
AGGCAGGGAA ACTCGAAGGA GGTCGAGAAA GTGCGCAAGA ACACCGTCTC CTACAAGTCC 
ATCAGCCAAA TGCCGCGCGA GCCGGAAGTC AAGGAGCTCA AGCTTTACGT GGATAAGAAG 
TACGAGACCG TCATCATGCC AGTGTTCGGC ATCCAGGTGC CGTTCCATAT ATCCACCATC 
AAGAACATCT CGCAGTCGGT GGAGGGCGAG TACACGTACT TGCGTATCAA CTTCTTTCAT 
CCCGGCGCCA CAATGGGTCG CAACGAAGGC GGACTCTATC CTCAGCCAGA GGCCACATTT 
GTCAAGGAAG TTACATACCG CAGCTCTAAT GTTAAGGAAC ACGGGGAGGT GGGAGCTCCA 
TCCGCAAATC TAAACAATGC TTTCCGGCTG ATCAAGGAGG TGCAGAAGCG CTTCAAGACC 
CGCGAGGCTG AGGAGCGCGA AAAGGAGGAT CTTGTGAAGC AAGACACACT CATTCTGTCC 
CAGAACAAGG GTAATCCCAA GCTGAAGGAC CTATACATTC GGCCCAACAT CGTGACCAAG 
CGCATGACGG GCAGCCTGGA AGCGCACAGC AATGGATTCC GCTACATCTC CGTGCGCGGC 
GACAAGGTGG ACATCTTGTA CAACAACATC AAAAGCGCGT TTTTCCAGCC CTGCGATGGC 
GAGATGATCA TTCTTCTGCA TTTCCACCTG AAGTACGCCA TTATGTTTGG CAAAAAGAAG 
CACGTGGATG TACAGTTCTA CACGGAAGTG GGAGAGATCA CCACGGATCT GGGCAAGCAC 
CAGCACATGC ACGACCGCGA CGACTTGGCT GCTGAGCAGG CAGAACGTGA GCTGCGGCAC 
AAGCTCAAGA CTGCCTTCAA GAGCTTCTGC GAGAAGGTGG AAACCATGAC CAAGTCGGTA 
GTGGAGTTCG ACACGCCGTT CCGTGAGTTG GGATTCCCTG GAGCACCCTT CCGCAGCACG 
GTGACGCTGC AACCCACCTC GGGCTCCCTG GTCAATCTGA CGGAATGGCC ACCATTTGTC 
ATCACGCTGG ACGATGTGGA GTTGGTGCAC TTCGAGCGCG TTCAGTTCCA CTTGCGCAAC 
TTCGATATGA TATTTGTGTT CAAGGAGTAC AATAAGAAGG TTGCCATGGT GAACGCCATT 
CCTATGAACA TGCTGGATCA CGTGAAGGAG TGGCTCAACT CCTGCGACAT CCGCTATTCG 
GAGGGTGTTC AATCCCTGAA CTGGCAAAAG ATCATGAAGA CCATCACCGA CGATCCTGAG 
GGATTCTTTG AACAAGGCGG CTGGACATTC TTGGACCCGG AATCGGGCAG CGAAGGCGAA 
AACGAAACCG CCGAATCCGA AGAGGACGAG GCCTACAATC CCACCGACGC CGAATCGGAT 
GAGGAGTCGG ACGAAGATTC CGAGTACTCA GAGGCATCAG AAGACAGCGA GGAAAGCGAT 
GAGGACCTTG GCTCTGATGA GGAATCTGGC AAGGATTGGT CCGATCTGGA GCGCGAGGCT 
GCTGAGGAAG ACCGCAACCA CGATTATGCC GCAGATGACA AACCACGCAA CGGCAAATTC 
GATTCGAAGA AGCACGGCAA GAGTTCAAAG CACAGCCCGT CGAAGTCGTC GAAGGACAAG 
TACAACAGCC GGGACAAGCA TCATTCCTCC TCATCGTCCG GAAACAAGAG CAGCAGCAAG 
GACAAGGATC GCAAGCGCTC GCGGGATGAC AGTCGCGACA ATGGGCACAA GTCGAAGAAG 
TCGCGACATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dre4 ( NM_057262.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476610.2 Drosophila melanogaster dre4, transcript variant A (dre4), mRNA.

Price & Availability↑

CloneID	OFa17244
Clone ID Related Accession (Same CDS sequence)	NM_057262.4
Accession Version	NM_057262.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dre4, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_057262.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGAGCT TTGTGCTGGA CAAGGAGGCG TTCGTGCGCC GCGTGAAACG CCTCTACACG 
GAATGGAGGG CTCCCAGCAT CGGACACGAT GACGCGCTCA GGAATCTGGA CTGCATCATG 
AGCATCGTGG GCGTCGAAGA GGATGTCATG TACTCCAAGT CCATGGCCCT GCAGCTCTGG 
CTGCTGGGCT ACGAGCTGAC GGACACCATC TCAGTGTTTT GCTCGGATGC CGTGTATTTT 
CTCACTAGCA AAAAGAAAAT AGAATTCCTG AAGCAGACGC AGAACATCAC CGAAGAAGGT 
TTTCCGGAAA TCAATCTGCT TGTTAGGGAT CGGACGGACA AGGACCAGGG CAACTTCGAG 
AAGCTCATCA AAGCCCTGCA AAACTCGAAG AAGGGCAAGC GTTTGGGCGT CTTCGCCAAG 
GACGCCTATC CCGGGGAGTT CAGCGAAGCA TGGAAGAAAT CCCTGACGGC GTCCAAGTTC 
GAGCATGTGG ACATTAGCAC TATTATCGCC TACCTCATGT GTCCCAAGGA TGAGTCGGAG 
ATCAACAACA TACGCAAGGC ATCGCTAGTG TCAATGGACA TTTTCAACAA GTATTTGAAA 
GATGAGATCA TGGACATTAT TGATTCGGAT AGGAAAGTCA AACACAACAA GCTCTCAGAT 
GGCTGCGAGG CTGCCATTGG CGAGAAGAAG TACACCAGTG GTCTGGATCC CAGACTCCTC 
GATATGGCCT ACCCACCCAT CATCCAATCG GGAGGAGCCT ACAGCCTGAA GTTCTCCGCG 
GTGGCGGACA AAAATCCCTT GCACTTCGGT GTGATTGTGT GCTCCTTGGG CGCCCGCTAC 
AAGTCGTATT GCTCAAATAT ATCGCGCACC TTTCTGGTGA ATCCCACCGA GGCCATGCAG 
GAGAACTACA CATTTCTGGT AAGTGTGCAG GAGGAGATTC TCAAGCTGCT GGTTCCGGGC 
ACAAAGCTGT GCGACGTCTA CGAAAAGACC CTGGACTTCG TGAAGAAGGA GAAGCCGAGT 
ATGGTGGACA ATCTGCCCAA AAGCTTTGGT TTCGCCATGG GCCTAGAGTT CCGGGAGAAC 
TCCATTGTCA TCGGACCCAA ATGCCAGGCG CTACTCAAGA AGAATATGGT CTTTAATTTG 
CACGTGGGCA TCTCGAATCT GACCAATCCC GAAGCCACCG ACAAAGAGGG CAAGAACTAC 
GCCCTCTTCA TCGGAGACAC TGTGCTAGTA GGCGAACAAT CCCCAGCCAG TGTGATGACT 
CCGTCCAAGA AGAAGATCAA GAACGTCGGC ATCTTTATCA AGGACGACAG CGACGAGGAA 
GATGTGGACG ACAAGAAGAC CGCTAAAGAA GATCAGGGCA CCGAGATCCT GGGCCGCAGC 
AAGCGCAACG CGGTGCTGGA GTCAAAGCTG CGAAACGAGA TCAACACGGA GGAGAAGCGC 
AAGGAGCACC AGCGCGAGCT GGCTCAGCAG CTGAACGAGC GCGCCAAGGA TCGGCTGGCC 
AGGCAGGGAA ACTCGAAGGA GGTCGAGAAA GTGCGCAAGA ACACCGTCTC CTACAAGTCC 
ATCAGCCAAA TGCCGCGCGA GCCGGAAGTC AAGGAGCTCA AGCTTTACGT GGATAAGAAG 
TACGAGACCG TCATCATGCC AGTGTTCGGC ATCCAGGTGC CGTTCCATAT ATCCACCATC 
AAGAACATCT CGCAGTCGGT GGAGGGCGAG TACACGTACT TGCGTATCAA CTTCTTTCAT 
CCCGGCGCCA CAATGGGTCG CAACGAAGGC GGACTCTATC CTCAGCCAGA GGCCACATTT 
GTCAAGGAAG TTACATACCG CAGCTCTAAT GTTAAGGAAC ACGGGGAGGT GGGAGCTCCA 
TCCGCAAATC TAAACAATGC TTTCCGGCTG ATCAAGGAGG TGCAGAAGCG CTTCAAGACC 
CGCGAGGCTG AGGAGCGCGA AAAGGAGGAT CTTGTGAAGC AAGACACACT CATTCTGTCC 
CAGAACAAGG GTAATCCCAA GCTGAAGGAC CTATACATTC GGCCCAACAT CGTGACCAAG 
CGCATGACGG GCAGCCTGGA AGCGCACAGC AATGGATTCC GCTACATCTC CGTGCGCGGC 
GACAAGGTGG ACATCTTGTA CAACAACATC AAAAGCGCGT TTTTCCAGCC CTGCGATGGC 
GAGATGATCA TTCTTCTGCA TTTCCACCTG AAGTACGCCA TTATGTTTGG CAAAAAGAAG 
CACGTGGATG TACAGTTCTA CACGGAAGTG GGAGAGATCA CCACGGATCT GGGCAAGCAC 
CAGCACATGC ACGACCGCGA CGACTTGGCT GCTGAGCAGG CAGAACGTGA GCTGCGGCAC 
AAGCTCAAGA CTGCCTTCAA GAGCTTCTGC GAGAAGGTGG AAACCATGAC CAAGTCGGTA 
GTGGAGTTCG ACACGCCGTT CCGTGAGTTG GGATTCCCTG GAGCACCCTT CCGCAGCACG 
GTGACGCTGC AACCCACCTC GGGCTCCCTG GTCAATCTGA CGGAATGGCC ACCATTTGTC 
ATCACGCTGG ACGATGTGGA GTTGGTGCAC TTCGAGCGCG TTCAGTTCCA CTTGCGCAAC 
TTCGATATGA TATTTGTGTT CAAGGAGTAC AATAAGAAGG TTGCCATGGT GAACGCCATT 
CCTATGAACA TGCTGGATCA CGTGAAGGAG TGGCTCAACT CCTGCGACAT CCGCTATTCG 
GAGGGTGTTC AATCCCTGAA CTGGCAAAAG ATCATGAAGA CCATCACCGA CGATCCTGAG 
GGATTCTTTG AACAAGGCGG CTGGACATTC TTGGACCCGG AATCGGGCAG CGAAGGCGAA 
AACGAAACCG CCGAATCCGA AGAGGACGAG GCCTACAATC CCACCGACGC CGAATCGGAT 
GAGGAGTCGG ACGAAGATTC CGAGTACTCA GAGGCATCAG AAGACAGCGA GGAAAGCGAT 
GAGGACCTTG GCTCTGATGA GGAATCTGGC AAGGATTGGT CCGATCTGGA GCGCGAGGCT 
GCTGAGGAAG ACCGCAACCA CGATTATGCC GCAGATGACA AACCACGCAA CGGCAAATTC 
GATTCGAAGA AGCACGGCAA GAGTTCAAAG CACAGTCGCC GCGACCGCGA GGAGGCTCGA 
TCGTCGTCGC ACAGTAAAAA GCACAAGTCG AACTCCTCCT CGTCCTCTTC GCATTTAAAG 
TCCTCCAGCT CCAAACATGG CAGCTCATCT AGCCCGTCGA AGTCGTCGAA GGACAAGTAC 
AACAGCCGGG ACAAGCATCA TTCCTCCTCA TCGTCCGGAA ACAAGAGCAG CAGCAAGGAC 
AAGGATCGCA AGCGCTCGCG GGATGACAGT CGCGACAATG GGCACAAGTC GAAGAAGTCG 
CGACATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476610.2
CDS	135..3503
Translation	MSSFVLDKEAFVRRVKRLYTEWRAPSIGHDDALRNLDCIMSIVGVEEDVMYSKSMALQLWLLGYELTDTISVFCSDAVYFLTSKKKIEFLKQTQNITEEGFPEINLLVRDRTDKDQGNFEKLIKALQNSKKGKRLGVFAKDAYPGEFSEAWKKSLTASKFEHVDISTIIAYLMCPKDESEINNIRKASLVSMDIFNKYLKDEIMDIIDSDRKVKHNKLSDGCEAAIGEKKYTSGLDPRLLDMAYPPIIQSGGAYSLKFSAVADKNPLHFGVIVCSLGARYKSYCSNISRTFLVNPTEAMQENYTFLVSVQEEILKLLVPGTKLCDVYEKTLDFVKKEKPSMVDNLPKSFGFAMGLEFRENSIVIGPKCQALLKKNMVFNLHVGISNLTNPEATDKEGKNYALFIGDTVLVGEQSPASVMTPSKKKIKNVGIFIKDDSDEEDVDDKKTAKEDQGTEILGRSKRNAVLESKLRNEINTEEKRKEHQRELAQQLNERAKDRLARQGNSKEVEKVRKNTVSYKSISQMPREPEVKELKLYVDKKYETVIMPVFGIQVPFHISTIKNISQSVEGEYTYLRINFFHPGATMGRNEGGLYPQPEATFVKEVTYRSSNVKEHGEVGAPSANLNNAFRLIKEVQKRFKTREAEEREKEDLVKQDTLILSQNKGNPKLKDLYIRPNIVTKRMTGSLEAHSNGFRYISVRGDKVDILYNNIKSAFFQPCDGEMIILLHFHLKYAIMFGKKKHVDVQFYTEVGEITTDLGKHQHMHDRDDLAAEQAERELRHKLKTAFKSFCEKVETMTKSVVEFDTPFRELGFPGAPFRSTVTLQPTSGSLVNLTEWPPFVITLDDVELVHFERVQFHLRNFDMIFVFKEYNKKVAMVNAIPMNMLDHVKEWLNSCDIRYSEGVQSLNWQKIMKTITDDPEGFFEQGGWTFLDPESGSEGENETAESEEDEAYNPTDAESDEESDEDSEYSEASEDSEESDEDLGSDEESGKDWSDLEREAAEEDRNHDYAADDKPRNGKFDSKKHGKSSKHSRRDREEARSSSHSKKHKSNSSSSSSHLKSSSSKHGSSSSPSKSSKDKYNSRDKHHSSSSSGNKSSSKDKDRKRSRDDSRDNGHKSKKSRH

Target ORF information:

RefSeq Version	NM_057262.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dre4, transcript variant A (dre4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057262.4

ORF Insert Sequence:

ATGTCGAGCT TTGTGCTGGA CAAGGAGGCG TTCGTGCGCC GCGTGAAACG CCTCTACACG 
GAATGGAGGG CTCCCAGCAT CGGACACGAT GACGCGCTCA GGAATCTGGA CTGCATCATG 
AGCATCGTGG GCGTCGAAGA GGATGTCATG TACTCCAAGT CCATGGCCCT GCAGCTCTGG 
CTGCTGGGCT ACGAGCTGAC GGACACCATC TCAGTGTTTT GCTCGGATGC CGTGTATTTT 
CTCACTAGCA AAAAGAAAAT AGAATTCCTG AAGCAGACGC AGAACATCAC CGAAGAAGGT 
TTTCCGGAAA TCAATCTGCT TGTTAGGGAT CGGACGGACA AGGACCAGGG CAACTTCGAG 
AAGCTCATCA AAGCCCTGCA AAACTCGAAG AAGGGCAAGC GTTTGGGCGT CTTCGCCAAG 
GACGCCTATC CCGGGGAGTT CAGCGAAGCA TGGAAGAAAT CCCTGACGGC GTCCAAGTTC 
GAGCATGTGG ACATTAGCAC TATTATCGCC TACCTCATGT GTCCCAAGGA TGAGTCGGAG 
ATCAACAACA TACGCAAGGC ATCGCTAGTG TCAATGGACA TTTTCAACAA GTATTTGAAA 
GATGAGATCA TGGACATTAT TGATTCGGAT AGGAAAGTCA AACACAACAA GCTCTCAGAT 
GGCTGCGAGG CTGCCATTGG CGAGAAGAAG TACACCAGTG GTCTGGATCC CAGACTCCTC 
GATATGGCCT ACCCACCCAT CATCCAATCG GGAGGAGCCT ACAGCCTGAA GTTCTCCGCG 
GTGGCGGACA AAAATCCCTT GCACTTCGGT GTGATTGTGT GCTCCTTGGG CGCCCGCTAC 
AAGTCGTATT GCTCAAATAT ATCGCGCACC TTTCTGGTGA ATCCCACCGA GGCCATGCAG 
GAGAACTACA CATTTCTGGT AAGTGTGCAG GAGGAGATTC TCAAGCTGCT GGTTCCGGGC 
ACAAAGCTGT GCGACGTCTA CGAAAAGACC CTGGACTTCG TGAAGAAGGA GAAGCCGAGT 
ATGGTGGACA ATCTGCCCAA AAGCTTTGGT TTCGCCATGG GCCTAGAGTT CCGGGAGAAC 
TCCATTGTCA TCGGACCCAA ATGCCAGGCG CTACTCAAGA AGAATATGGT CTTTAATTTG 
CACGTGGGCA TCTCGAATCT GACCAATCCC GAAGCCACCG ACAAAGAGGG CAAGAACTAC 
GCCCTCTTCA TCGGAGACAC TGTGCTAGTA GGCGAACAAT CCCCAGCCAG TGTGATGACT 
CCGTCCAAGA AGAAGATCAA GAACGTCGGC ATCTTTATCA AGGACGACAG CGACGAGGAA 
GATGTGGACG ACAAGAAGAC CGCTAAAGAA GATCAGGGCA CCGAGATCCT GGGCCGCAGC 
AAGCGCAACG CGGTGCTGGA GTCAAAGCTG CGAAACGAGA TCAACACGGA GGAGAAGCGC 
AAGGAGCACC AGCGCGAGCT GGCTCAGCAG CTGAACGAGC GCGCCAAGGA TCGGCTGGCC 
AGGCAGGGAA ACTCGAAGGA GGTCGAGAAA GTGCGCAAGA ACACCGTCTC CTACAAGTCC 
ATCAGCCAAA TGCCGCGCGA GCCGGAAGTC AAGGAGCTCA AGCTTTACGT GGATAAGAAG 
TACGAGACCG TCATCATGCC AGTGTTCGGC ATCCAGGTGC CGTTCCATAT ATCCACCATC 
AAGAACATCT CGCAGTCGGT GGAGGGCGAG TACACGTACT TGCGTATCAA CTTCTTTCAT 
CCCGGCGCCA CAATGGGTCG CAACGAAGGC GGACTCTATC CTCAGCCAGA GGCCACATTT 
GTCAAGGAAG TTACATACCG CAGCTCTAAT GTTAAGGAAC ACGGGGAGGT GGGAGCTCCA 
TCCGCAAATC TAAACAATGC TTTCCGGCTG ATCAAGGAGG TGCAGAAGCG CTTCAAGACC 
CGCGAGGCTG AGGAGCGCGA AAAGGAGGAT CTTGTGAAGC AAGACACACT CATTCTGTCC 
CAGAACAAGG GTAATCCCAA GCTGAAGGAC CTATACATTC GGCCCAACAT CGTGACCAAG 
CGCATGACGG GCAGCCTGGA AGCGCACAGC AATGGATTCC GCTACATCTC CGTGCGCGGC 
GACAAGGTGG ACATCTTGTA CAACAACATC AAAAGCGCGT TTTTCCAGCC CTGCGATGGC 
GAGATGATCA TTCTTCTGCA TTTCCACCTG AAGTACGCCA TTATGTTTGG CAAAAAGAAG 
CACGTGGATG TACAGTTCTA CACGGAAGTG GGAGAGATCA CCACGGATCT GGGCAAGCAC 
CAGCACATGC ACGACCGCGA CGACTTGGCT GCTGAGCAGG CAGAACGTGA GCTGCGGCAC 
AAGCTCAAGA CTGCCTTCAA GAGCTTCTGC GAGAAGGTGG AAACCATGAC CAAGTCGGTA 
GTGGAGTTCG ACACGCCGTT CCGTGAGTTG GGATTCCCTG GAGCACCCTT CCGCAGCACG 
GTGACGCTGC AACCCACCTC GGGCTCCCTG GTCAATCTGA CGGAATGGCC ACCATTTGTC 
ATCACGCTGG ACGATGTGGA GTTGGTGCAC TTCGAGCGCG TTCAGTTCCA CTTGCGCAAC 
TTCGATATGA TATTTGTGTT CAAGGAGTAC AATAAGAAGG TTGCCATGGT GAACGCCATT 
CCTATGAACA TGCTGGATCA CGTGAAGGAG TGGCTCAACT CCTGCGACAT CCGCTATTCG 
GAGGGTGTTC AATCCCTGAA CTGGCAAAAG ATCATGAAGA CCATCACCGA CGATCCTGAG 
GGATTCTTTG AACAAGGCGG CTGGACATTC TTGGACCCGG AATCGGGCAG CGAAGGCGAA 
AACGAAACCG CCGAATCCGA AGAGGACGAG GCCTACAATC CCACCGACGC CGAATCGGAT 
GAGGAGTCGG ACGAAGATTC CGAGTACTCA GAGGCATCAG AAGACAGCGA GGAAAGCGAT 
GAGGACCTTG GCTCTGATGA GGAATCTGGC AAGGATTGGT CCGATCTGGA GCGCGAGGCT 
GCTGAGGAAG ACCGCAACCA CGATTATGCC GCAGATGACA AACCACGCAA CGGCAAATTC 
GATTCGAAGA AGCACGGCAA GAGTTCAAAG CACAGTCGCC GCGACCGCGA GGAGGCTCGA 
TCGTCGTCGC ACAGTAAAAA GCACAAGTCG AACTCCTCCT CGTCCTCTTC GCATTTAAAG 
TCCTCCAGCT CCAAACATGG CAGCTCATCT AGCCCGTCGA AGTCGTCGAA GGACAAGTAC 
AACAGCCGGG ACAAGCATCA TTCCTCCTCA TCGTCCGGAA ACAAGAGCAG CAGCAAGGAC 
AAGGATCGCA AGCGCTCGCG GGATGACAGT CGCGACAATG GGCACAAGTC GAAGAAGTCG 
CGACATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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dre4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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