Cht2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cht2
Entrez Gene ID	38223
Full Name	Chitinase 2
Synonyms	CG2054,CHT2,DmCHT2,DmCht2,Dmel\CG2054,cht2
General protein information	Preferred Names Chitinase 2 Names CG2054 gene product from transcript CG2054-RB CG2054-PA CG2054-PB CG2054-PC Cht2-PA Cht2-PB Cht2-PC chitinase 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 62B1-62B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274353.1	NP_001261282.1	chitinase 2, isoform C
NM_001259621.2	NP_001246550.1	chitinase 2, isoform B
NM_057950.4	NP_477298.2	chitinase 2, isoform A

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_11231	XP_003714255.1
Neurospora crassa	NCU03026	XP_965412.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005634	XP_315650.4
Drosophila melanogaster (fruit fly)	Cht2	NP_001246550.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cht2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cht2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17221	NM_057950.4 Latest version!	Drosophila melanogaster chitinase 2, transcript variant A (Cht2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa17221	NM_001259621.2 Latest version!	Drosophila melanogaster chitinase 2, transcript variant B (Cht2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa17221	NM_001274353.1 Latest version!	Drosophila melanogaster chitinase 2, transcript variant C (Cht2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cht2 ( NM_001274353.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261282.1 Drosophila melanogaster chitinase 2, transcript variant C (Cht2), mRNA.

Price & Availability↑

CloneID	OFa17221
Clone ID Related Accession (Same CDS sequence)	NM_001274353.1 , NM_001259621.2 , NM_057950.4
Accession Version	NM_001274353.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chitinase 2, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCTGA GGAGCAGACT TAGTGGGGAA GCACCGCAGC TGTGGCTGCT CCTCCTCCTG 
GCCTCCACTG CGAGCAGCTT GTGGGCTTCG GTGGCCGCCA GGACAGGACC CCTGCACGAC 
AAGGTGGTGG TTTGCTACGT TTCCACATGG GCTGTTTACC GGCCGGAGCA GGGTGCCTAT 
GCCATCGAGA ACTTCGACCC CAATTTGTGC ACCCATGTGG TGTACGCCTT TGCCGGCCTG 
GACATCACCC AGGCAGCCAT CAAGTCGTTG GATCCCTGGC AGGACCTAAA GGAGGAGTAC 
GGCAAGGGTG GCTACGAGAA AATGACGGGT CTGAAGCGCA GCCATCCGCA TCTGAAGGTC 
AGCCTGGCCA TCGGCGGCTG GAACGAGGGA TCGGCCAACT ACTCCACCCT GGTGGCCAAC 
AATCTGCTGC GCGGCAGGTT CGTCAAGCAG GTGTCCAGTT TCATACGCAA GTACAACTTC 
GACGGGCTGG ATCTCGACTG GGAGTATCCC ACGCAGCGGA AGGGCAAGCC CGCGGATCGC 
GAGAACTTCG TTCTGCTGAC CAAGGAGCTG CGCGAGGAGT TCGACGAGCA CGGCCTGCTG 
CTGACCTCCG CCATCGGAGC CTCCAAGAAG GTGATCGACG AGGCCTATGA CGTGCGGCAG 
ATCTCGCGTT ACTTGGACTA CCTGCACATC ATGTGCTACG ACTACCACGG CAGCTGGGAC 
CGCCGAGTTG GTTACAATGC TCCGCTCACG GCGCCCGCGG ATGATCCGCT GAGTGTGAAA 
TTCTCCATTG ACTACCTGCT GAAACTGGGC GCCCCGCCGG AGAAGCTGGT GATGGGCCTG 
CCCTTCTACG GACGCACCTT CAAGACGTTG GCCAGTGGAT TCCTGAACGA CGTCAGCGAA 
GGCGTAGGCT TCAAGGGACC CTACACGCGG GAGGACGGCT TCCTGGGCTA CAACGAGATC 
TGCCAGACAC TGAGCAACCA GACCTCCGGA TGGACAAGGG AGTGGGATCC GCAAACCAGC 
CAGGTGCTGG CCAAGTCCGA GCGAAACGTC TTCACCCAGG AGATCAACGT GGTGACCTAC 
GACAGCTCCC GCTCCATCGC CAACAAGGTG TTGTTCGCCA TGTCCAAGCG GCTTGCGGGT 
GTGATGGTGT GGTCCGTGGA CACGGACGAC TTCCTGGGTA ACTGCAAGCT GGACGAGGAC 
ACGTACGAGG ACTTCCAGAA GGTGACTGCC GCGCCCAAGA GATCGAGCCA GAACTATCCC 
CTTCTGCGGA CCATCAACGA GGCCACCATG CTGGCCGTGG ACGAGCTGGC TGTGCCGGAG 
CCGCAGCCGG ATGACTCCGA GAACGAGATT CCGCACGGCA GCATAGCGGA TCGCAAGAAT 
GCCGGCGCTT CTATGGTCAG TCTGGGCCTC GGAGTCACCG CTGTCTTCAT GCTCCTGCAC 
CGGTTGGCCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261282.1
CDS	367..1821
Translation	MTLRSRLSGEAPQLWLLLLLASTASSLWASVAARTGPLHDKVVVCYVSTWAVYRPEQGAYAIENFDPNLCTHVVYAFAGLDITQAAIKSLDPWQDLKEEYGKGGYEKMTGLKRSHPHLKVSLAIGGWNEGSANYSTLVANNLLRGRFVKQVSSFIRKYNFDGLDLDWEYPTQRKGKPADRENFVLLTKELREEFDEHGLLLTSAIGASKKVIDEAYDVRQISRYLDYLHIMCYDYHGSWDRRVGYNAPLTAPADDPLSVKFSIDYLLKLGAPPEKLVMGLPFYGRTFKTLASGFLNDVSEGVGFKGPYTREDGFLGYNEICQTLSNQTSGWTREWDPQTSQVLAKSERNVFTQEINVVTYDSSRSIANKVLFAMSKRLAGVMVWSVDTDDFLGNCKLDEDTYEDFQKVTAAPKRSSQNYPLLRTINEATMLAVDELAVPEPQPDDSENEIPHGSIADRKNAGASMVSLGLGVTAVFMLLHRLAQ

Target ORF information:

RefSeq Version	NM_001274353.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chitinase 2, transcript variant C (Cht2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274353.1

ORF Insert Sequence:

ATGACGCTGA GGAGCAGACT TAGTGGGGAA GCACCGCAGC TGTGGCTGCT CCTCCTCCTG 
GCCTCCACTG CGAGCAGCTT GTGGGCTTCG GTGGCCGCCA GGACAGGACC CCTGCACGAC 
AAGGTGGTGG TTTGCTACGT TTCCACATGG GCTGTTTACC GGCCGGAGCA GGGTGCCTAT 
GCCATCGAGA ACTTCGACCC CAATTTGTGC ACCCATGTGG TGTACGCCTT TGCCGGCCTG 
GACATCACCC AGGCAGCCAT CAAGTCGTTG GATCCCTGGC AGGACCTAAA GGAGGAGTAC 
GGCAAGGGTG GCTACGAGAA AATGACGGGT CTGAAGCGCA GCCATCCGCA TCTGAAGGTC 
AGCCTGGCCA TCGGCGGCTG GAACGAGGGA TCGGCCAACT ACTCCACCCT GGTGGCCAAC 
AATCTGCTGC GCGGCAGGTT CGTCAAGCAG GTGTCCAGTT TCATACGCAA GTACAACTTC 
GACGGGCTGG ATCTCGACTG GGAGTATCCC ACGCAGCGGA AGGGCAAGCC CGCGGATCGC 
GAGAACTTCG TTCTGCTGAC CAAGGAGCTG CGCGAGGAGT TCGACGAGCA CGGCCTGCTG 
CTGACCTCCG CCATCGGAGC CTCCAAGAAG GTGATCGACG AGGCCTATGA CGTGCGGCAG 
ATCTCGCGTT ACTTGGACTA CCTGCACATC ATGTGCTACG ACTACCACGG CAGCTGGGAC 
CGCCGAGTTG GTTACAATGC TCCGCTCACG GCGCCCGCGG ATGATCCGCT GAGTGTGAAA 
TTCTCCATTG ACTACCTGCT GAAACTGGGC GCCCCGCCGG AGAAGCTGGT GATGGGCCTG 
CCCTTCTACG GACGCACCTT CAAGACGTTG GCCAGTGGAT TCCTGAACGA CGTCAGCGAA 
GGCGTAGGCT TCAAGGGACC CTACACGCGG GAGGACGGCT TCCTGGGCTA CAACGAGATC 
TGCCAGACAC TGAGCAACCA GACCTCCGGA TGGACAAGGG AGTGGGATCC GCAAACCAGC 
CAGGTGCTGG CCAAGTCCGA GCGAAACGTC TTCACCCAGG AGATCAACGT GGTGACCTAC 
GACAGCTCCC GCTCCATCGC CAACAAGGTG TTGTTCGCCA TGTCCAAGCG GCTTGCGGGT 
GTGATGGTGT GGTCCGTGGA CACGGACGAC TTCCTGGGTA ACTGCAAGCT GGACGAGGAC 
ACGTACGAGG ACTTCCAGAA GGTGACTGCC GCGCCCAAGA GATCGAGCCA GAACTATCCC 
CTTCTGCGGA CCATCAACGA GGCCACCATG CTGGCCGTGG ACGAGCTGGC TGTGCCGGAG 
CCGCAGCCGG ATGACTCCGA GAACGAGATT CCGCACGGCA GCATAGCGGA TCGCAAGAAT 
GCCGGCGCTT CTATGGTCAG TCTGGGCCTC GGAGTCACCG CTGTCTTCAT GCTCCTGCAC 
CGGTTGGCCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cht2 ( NM_001259621.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246550.1 Drosophila melanogaster chitinase 2, transcript variant B (Cht2), mRNA.

Price & Availability↑

CloneID	OFa17221
Clone ID Related Accession (Same CDS sequence)	NM_001274353.1 , NM_001259621.2 , NM_057950.4
Accession Version	NM_001259621.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chitinase 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001259621.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCTGA GGAGCAGACT TAGTGGGGAA GCACCGCAGC TGTGGCTGCT CCTCCTCCTG 
GCCTCCACTG CGAGCAGCTT GTGGGCTTCG GTGGCCGCCA GGACAGGACC CCTGCACGAC 
AAGGTGGTGG TTTGCTACGT TTCCACATGG GCTGTTTACC GGCCGGAGCA GGGTGCCTAT 
GCCATCGAGA ACTTCGACCC CAATTTGTGC ACCCATGTGG TGTACGCCTT TGCCGGCCTG 
GACATCACCC AGGCAGCCAT CAAGTCGTTG GATCCCTGGC AGGACCTAAA GGAGGAGTAC 
GGCAAGGGTG GCTACGAGAA AATGACGGGT CTGAAGCGCA GCCATCCGCA TCTGAAGGTC 
AGCCTGGCCA TCGGCGGCTG GAACGAGGGA TCGGCCAACT ACTCCACCCT GGTGGCCAAC 
AATCTGCTGC GCGGCAGGTT CGTCAAGCAG GTGTCCAGTT TCATACGCAA GTACAACTTC 
GACGGGCTGG ATCTCGACTG GGAGTATCCC ACGCAGCGGA AGGGCAAGCC CGCGGATCGC 
GAGAACTTCG TTCTGCTGAC CAAGGAGCTG CGCGAGGAGT TCGACGAGCA CGGCCTGCTG 
CTGACCTCCG CCATCGGAGC CTCCAAGAAG GTGATCGACG AGGCCTATGA CGTGCGGCAG 
ATCTCGCGTT ACTTGGACTA CCTGCACATC ATGTGCTACG ACTACCACGG CAGCTGGGAC 
CGCCGAGTTG GTTACAATGC TCCGCTCACG GCGCCCGCGG ATGATCCGCT GAGTGTGAAA 
TTCTCCATTG ACTACCTGCT GAAACTGGGC GCCCCGCCGG AGAAGCTGGT GATGGGCCTG 
CCCTTCTACG GACGCACCTT CAAGACGTTG GCCAGTGGAT TCCTGAACGA CGTCAGCGAA 
GGCGTAGGCT TCAAGGGACC CTACACGCGG GAGGACGGCT TCCTGGGCTA CAACGAGATC 
TGCCAGACAC TGAGCAACCA GACCTCCGGA TGGACAAGGG AGTGGGATCC GCAAACCAGC 
CAGGTGCTGG CCAAGTCCGA GCGAAACGTC TTCACCCAGG AGATCAACGT GGTGACCTAC 
GACAGCTCCC GCTCCATCGC CAACAAGGTG TTGTTCGCCA TGTCCAAGCG GCTTGCGGGT 
GTGATGGTGT GGTCCGTGGA CACGGACGAC TTCCTGGGTA ACTGCAAGCT GGACGAGGAC 
ACGTACGAGG ACTTCCAGAA GGTGACTGCC GCGCCCAAGA GATCGAGCCA GAACTATCCC 
CTTCTGCGGA CCATCAACGA GGCCACCATG CTGGCCGTGG ACGAGCTGGC TGTGCCGGAG 
CCGCAGCCGG ATGACTCCGA GAACGAGATT CCGCACGGCA GCATAGCGGA TCGCAAGAAT 
GCCGGCGCTT CTATGGTCAG TCTGGGCCTC GGAGTCACCG CTGTCTTCAT GCTCCTGCAC 
CGGTTGGCCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246550.1
CDS	931..2385
Translation	MTLRSRLSGEAPQLWLLLLLASTASSLWASVAARTGPLHDKVVVCYVSTWAVYRPEQGAYAIENFDPNLCTHVVYAFAGLDITQAAIKSLDPWQDLKEEYGKGGYEKMTGLKRSHPHLKVSLAIGGWNEGSANYSTLVANNLLRGRFVKQVSSFIRKYNFDGLDLDWEYPTQRKGKPADRENFVLLTKELREEFDEHGLLLTSAIGASKKVIDEAYDVRQISRYLDYLHIMCYDYHGSWDRRVGYNAPLTAPADDPLSVKFSIDYLLKLGAPPEKLVMGLPFYGRTFKTLASGFLNDVSEGVGFKGPYTREDGFLGYNEICQTLSNQTSGWTREWDPQTSQVLAKSERNVFTQEINVVTYDSSRSIANKVLFAMSKRLAGVMVWSVDTDDFLGNCKLDEDTYEDFQKVTAAPKRSSQNYPLLRTINEATMLAVDELAVPEPQPDDSENEIPHGSIADRKNAGASMVSLGLGVTAVFMLLHRLAQ

Target ORF information:

RefSeq Version	NM_001259621.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chitinase 2, transcript variant B (Cht2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259621.2

ORF Insert Sequence:

ATGACGCTGA GGAGCAGACT TAGTGGGGAA GCACCGCAGC TGTGGCTGCT CCTCCTCCTG 
GCCTCCACTG CGAGCAGCTT GTGGGCTTCG GTGGCCGCCA GGACAGGACC CCTGCACGAC 
AAGGTGGTGG TTTGCTACGT TTCCACATGG GCTGTTTACC GGCCGGAGCA GGGTGCCTAT 
GCCATCGAGA ACTTCGACCC CAATTTGTGC ACCCATGTGG TGTACGCCTT TGCCGGCCTG 
GACATCACCC AGGCAGCCAT CAAGTCGTTG GATCCCTGGC AGGACCTAAA GGAGGAGTAC 
GGCAAGGGTG GCTACGAGAA AATGACGGGT CTGAAGCGCA GCCATCCGCA TCTGAAGGTC 
AGCCTGGCCA TCGGCGGCTG GAACGAGGGA TCGGCCAACT ACTCCACCCT GGTGGCCAAC 
AATCTGCTGC GCGGCAGGTT CGTCAAGCAG GTGTCCAGTT TCATACGCAA GTACAACTTC 
GACGGGCTGG ATCTCGACTG GGAGTATCCC ACGCAGCGGA AGGGCAAGCC CGCGGATCGC 
GAGAACTTCG TTCTGCTGAC CAAGGAGCTG CGCGAGGAGT TCGACGAGCA CGGCCTGCTG 
CTGACCTCCG CCATCGGAGC CTCCAAGAAG GTGATCGACG AGGCCTATGA CGTGCGGCAG 
ATCTCGCGTT ACTTGGACTA CCTGCACATC ATGTGCTACG ACTACCACGG CAGCTGGGAC 
CGCCGAGTTG GTTACAATGC TCCGCTCACG GCGCCCGCGG ATGATCCGCT GAGTGTGAAA 
TTCTCCATTG ACTACCTGCT GAAACTGGGC GCCCCGCCGG AGAAGCTGGT GATGGGCCTG 
CCCTTCTACG GACGCACCTT CAAGACGTTG GCCAGTGGAT TCCTGAACGA CGTCAGCGAA 
GGCGTAGGCT TCAAGGGACC CTACACGCGG GAGGACGGCT TCCTGGGCTA CAACGAGATC 
TGCCAGACAC TGAGCAACCA GACCTCCGGA TGGACAAGGG AGTGGGATCC GCAAACCAGC 
CAGGTGCTGG CCAAGTCCGA GCGAAACGTC TTCACCCAGG AGATCAACGT GGTGACCTAC 
GACAGCTCCC GCTCCATCGC CAACAAGGTG TTGTTCGCCA TGTCCAAGCG GCTTGCGGGT 
GTGATGGTGT GGTCCGTGGA CACGGACGAC TTCCTGGGTA ACTGCAAGCT GGACGAGGAC 
ACGTACGAGG ACTTCCAGAA GGTGACTGCC GCGCCCAAGA GATCGAGCCA GAACTATCCC 
CTTCTGCGGA CCATCAACGA GGCCACCATG CTGGCCGTGG ACGAGCTGGC TGTGCCGGAG 
CCGCAGCCGG ATGACTCCGA GAACGAGATT CCGCACGGCA GCATAGCGGA TCGCAAGAAT 
GCCGGCGCTT CTATGGTCAG TCTGGGCCTC GGAGTCACCG CTGTCTTCAT GCTCCTGCAC 
CGGTTGGCCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cht2 ( NM_057950.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477298.2 Drosophila melanogaster chitinase 2, transcript variant A (Cht2), mRNA.

Price & Availability↑

CloneID	OFa17221
Clone ID Related Accession (Same CDS sequence)	NM_001274353.1 , NM_001259621.2 , NM_057950.4
Accession Version	NM_057950.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chitinase 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_057950.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCTGA GGAGCAGACT TAGTGGGGAA GCACCGCAGC TGTGGCTGCT CCTCCTCCTG 
GCCTCCACTG CGAGCAGCTT GTGGGCTTCG GTGGCCGCCA GGACAGGACC CCTGCACGAC 
AAGGTGGTGG TTTGCTACGT TTCCACATGG GCTGTTTACC GGCCGGAGCA GGGTGCCTAT 
GCCATCGAGA ACTTCGACCC CAATTTGTGC ACCCATGTGG TGTACGCCTT TGCCGGCCTG 
GACATCACCC AGGCAGCCAT CAAGTCGTTG GATCCCTGGC AGGACCTAAA GGAGGAGTAC 
GGCAAGGGTG GCTACGAGAA AATGACGGGT CTGAAGCGCA GCCATCCGCA TCTGAAGGTC 
AGCCTGGCCA TCGGCGGCTG GAACGAGGGA TCGGCCAACT ACTCCACCCT GGTGGCCAAC 
AATCTGCTGC GCGGCAGGTT CGTCAAGCAG GTGTCCAGTT TCATACGCAA GTACAACTTC 
GACGGGCTGG ATCTCGACTG GGAGTATCCC ACGCAGCGGA AGGGCAAGCC CGCGGATCGC 
GAGAACTTCG TTCTGCTGAC CAAGGAGCTG CGCGAGGAGT TCGACGAGCA CGGCCTGCTG 
CTGACCTCCG CCATCGGAGC CTCCAAGAAG GTGATCGACG AGGCCTATGA CGTGCGGCAG 
ATCTCGCGTT ACTTGGACTA CCTGCACATC ATGTGCTACG ACTACCACGG CAGCTGGGAC 
CGCCGAGTTG GTTACAATGC TCCGCTCACG GCGCCCGCGG ATGATCCGCT GAGTGTGAAA 
TTCTCCATTG ACTACCTGCT GAAACTGGGC GCCCCGCCGG AGAAGCTGGT GATGGGCCTG 
CCCTTCTACG GACGCACCTT CAAGACGTTG GCCAGTGGAT TCCTGAACGA CGTCAGCGAA 
GGCGTAGGCT TCAAGGGACC CTACACGCGG GAGGACGGCT TCCTGGGCTA CAACGAGATC 
TGCCAGACAC TGAGCAACCA GACCTCCGGA TGGACAAGGG AGTGGGATCC GCAAACCAGC 
CAGGTGCTGG CCAAGTCCGA GCGAAACGTC TTCACCCAGG AGATCAACGT GGTGACCTAC 
GACAGCTCCC GCTCCATCGC CAACAAGGTG TTGTTCGCCA TGTCCAAGCG GCTTGCGGGT 
GTGATGGTGT GGTCCGTGGA CACGGACGAC TTCCTGGGTA ACTGCAAGCT GGACGAGGAC 
ACGTACGAGG ACTTCCAGAA GGTGACTGCC GCGCCCAAGA GATCGAGCCA GAACTATCCC 
CTTCTGCGGA CCATCAACGA GGCCACCATG CTGGCCGTGG ACGAGCTGGC TGTGCCGGAG 
CCGCAGCCGG ATGACTCCGA GAACGAGATT CCGCACGGCA GCATAGCGGA TCGCAAGAAT 
GCCGGCGCTT CTATGGTCAG TCTGGGCCTC GGAGTCACCG CTGTCTTCAT GCTCCTGCAC 
CGGTTGGCCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477298.2
CDS	101..1555
Translation	MTLRSRLSGEAPQLWLLLLLASTASSLWASVAARTGPLHDKVVVCYVSTWAVYRPEQGAYAIENFDPNLCTHVVYAFAGLDITQAAIKSLDPWQDLKEEYGKGGYEKMTGLKRSHPHLKVSLAIGGWNEGSANYSTLVANNLLRGRFVKQVSSFIRKYNFDGLDLDWEYPTQRKGKPADRENFVLLTKELREEFDEHGLLLTSAIGASKKVIDEAYDVRQISRYLDYLHIMCYDYHGSWDRRVGYNAPLTAPADDPLSVKFSIDYLLKLGAPPEKLVMGLPFYGRTFKTLASGFLNDVSEGVGFKGPYTREDGFLGYNEICQTLSNQTSGWTREWDPQTSQVLAKSERNVFTQEINVVTYDSSRSIANKVLFAMSKRLAGVMVWSVDTDDFLGNCKLDEDTYEDFQKVTAAPKRSSQNYPLLRTINEATMLAVDELAVPEPQPDDSENEIPHGSIADRKNAGASMVSLGLGVTAVFMLLHRLAQ

Target ORF information:

RefSeq Version	NM_057950.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chitinase 2, transcript variant A (Cht2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057950.4

ORF Insert Sequence:

ATGACGCTGA GGAGCAGACT TAGTGGGGAA GCACCGCAGC TGTGGCTGCT CCTCCTCCTG 
GCCTCCACTG CGAGCAGCTT GTGGGCTTCG GTGGCCGCCA GGACAGGACC CCTGCACGAC 
AAGGTGGTGG TTTGCTACGT TTCCACATGG GCTGTTTACC GGCCGGAGCA GGGTGCCTAT 
GCCATCGAGA ACTTCGACCC CAATTTGTGC ACCCATGTGG TGTACGCCTT TGCCGGCCTG 
GACATCACCC AGGCAGCCAT CAAGTCGTTG GATCCCTGGC AGGACCTAAA GGAGGAGTAC 
GGCAAGGGTG GCTACGAGAA AATGACGGGT CTGAAGCGCA GCCATCCGCA TCTGAAGGTC 
AGCCTGGCCA TCGGCGGCTG GAACGAGGGA TCGGCCAACT ACTCCACCCT GGTGGCCAAC 
AATCTGCTGC GCGGCAGGTT CGTCAAGCAG GTGTCCAGTT TCATACGCAA GTACAACTTC 
GACGGGCTGG ATCTCGACTG GGAGTATCCC ACGCAGCGGA AGGGCAAGCC CGCGGATCGC 
GAGAACTTCG TTCTGCTGAC CAAGGAGCTG CGCGAGGAGT TCGACGAGCA CGGCCTGCTG 
CTGACCTCCG CCATCGGAGC CTCCAAGAAG GTGATCGACG AGGCCTATGA CGTGCGGCAG 
ATCTCGCGTT ACTTGGACTA CCTGCACATC ATGTGCTACG ACTACCACGG CAGCTGGGAC 
CGCCGAGTTG GTTACAATGC TCCGCTCACG GCGCCCGCGG ATGATCCGCT GAGTGTGAAA 
TTCTCCATTG ACTACCTGCT GAAACTGGGC GCCCCGCCGG AGAAGCTGGT GATGGGCCTG 
CCCTTCTACG GACGCACCTT CAAGACGTTG GCCAGTGGAT TCCTGAACGA CGTCAGCGAA 
GGCGTAGGCT TCAAGGGACC CTACACGCGG GAGGACGGCT TCCTGGGCTA CAACGAGATC 
TGCCAGACAC TGAGCAACCA GACCTCCGGA TGGACAAGGG AGTGGGATCC GCAAACCAGC 
CAGGTGCTGG CCAAGTCCGA GCGAAACGTC TTCACCCAGG AGATCAACGT GGTGACCTAC 
GACAGCTCCC GCTCCATCGC CAACAAGGTG TTGTTCGCCA TGTCCAAGCG GCTTGCGGGT 
GTGATGGTGT GGTCCGTGGA CACGGACGAC TTCCTGGGTA ACTGCAAGCT GGACGAGGAC 
ACGTACGAGG ACTTCCAGAA GGTGACTGCC GCGCCCAAGA GATCGAGCCA GAACTATCCC 
CTTCTGCGGA CCATCAACGA GGCCACCATG CTGGCCGTGG ACGAGCTGGC TGTGCCGGAG 
CCGCAGCCGG ATGACTCCGA GAACGAGATT CCGCACGGCA GCATAGCGGA TCGCAAGAAT 
GCCGGCGCTT CTATGGTCAG TCTGGGCCTC GGAGTCACCG CTGTCTTCAT GCTCCTGCAC 
CGGTTGGCCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Cht2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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