CG12099 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG12099
Entrez Gene ID	38181
Full Name	CG12099 gene product from transcript CG12099-RA
Synonyms	Dmel\CG12099
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 62A5-62A5

mRNA and Protein(s)

mRNA	Protein	Name
NM_139366.3	NP_647623.1	uncharacterized protein, isoform B
NM_167895.2	NP_728629.1	uncharacterized protein, isoform A

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG12099	NP_647623.1
Bos taurus (cattle)	RNF10	NP_001070337.1
Rattus norvegicus (Norway rat)	Rnf10	NP_001011904.1
Homo sapiens (human)	RNF10	NP_055683.3
Canis lupus familiaris (dog)	RNF10	XP_851332.2
Danio rerio (zebrafish)	rnf10	NP_001116727.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007954	XP_317518.4
	RNF10	XP_002798843.1
Mus musculus (house mouse)	Rnf10	NP_057907.2
Gallus gallus (chicken)	RNF10	XP_415267.2
Xenopus tropicalis (tropical clawed frog)	rnf10	NP_989388.1
Pan troglodytes (chimpanzee)	RNF10	XP_001161754.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG12099 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG12099 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17174	NM_139366.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG12099), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa17174	NM_167895.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG12099), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG12099 ( NM_139366.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_647623.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG12099), mRNA.

Price & Availability↑

CloneID	OFa17174
Clone ID Related Accession (Same CDS sequence)	NM_139366.3 , NM_167895.2
Accession Version	NM_139366.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_139366.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACAGCA ACAACAGCAG CAACAGCAAG AAGCAGCCTC AATCCTCTCA TCATGGCAAG 
TCCTCGTCTG CTGAATCGCA AAGGAAGTCC AGTGAGTTAC ATACCAACAG CAACAAGCAG 
CGACACAGCC GTCGAAGGAA TCAGGTTCCC ACACCGCGCA ACGATCAGCA ACAACAGCCA 
CAGCAGCCAG CAAAGCTACG TCCCAATGTG GACAAGCGTC CGAAGGCTCG CGGGTTTGGA 
GGCGGTTACG ATTTCCCTTC ACCCCGTGGC CAGGACGGGG CTTCAGGCAG CTCCCGACTA 
GCCTACTCCG CCACCGGCTA CTCACGTAGC GGTGACTTTG ACCATGAACT GAACTCTGTG 
TATACACAGG GCAGCAAGAA GCAGAATCTG AACCATCTGC TTAACTTCCA CTGCGTGCCC 
AGGGAACTGG AGCGAGGACA TCACCATCAG GCCCAACAGC ACCACGGCCT GAGTGGCCGA 
AAGCAGCGCT ACAACAAGGA GCAGTTCCTG CAGGCCAACT TTCAGTTCGT CATTAGATCG 
GGTGCAAAGG CTCAGGTTAA TGGCTCGCCG GATGCACTCA TCGATTGGAG CTACATCGAG 
CAGATCAACA TACAGACCAC TGAGGAGTTG CAGTGCCCCA TCTGCCTGTA CCCGCCTGTG 
GCTGCCAAGC TCACCAGATG CGGACACGCC TACTGTTGGC CCTGCTTGCT GCACTACCTC 
TCGCTGAGCG ACAAGACCTG GCGCAAGTGT CCTATCTGCT ATGACGCCAT CCACGCGGGA 
GATCTGAAGA GCTGCACCAT CGAACAGCTG AGGGATTTGC AAGTGGGCGA GAAGATTACC 
TTCCAGTTGA TGCGGCGTCG CAAGGGCTCC ATGTACATTG AAAATCATGT TGCAGGATTG 
GGCGAAACAA TTGAACGTTT CCCCTTTGTG TCCGCTGGTG AAGAGGCTAA ACGCTACTCA 
AAGTTTCTAA TAGCCAAGCG GATGGATGTG GCCGCCATCA TCGAGAGGGA GCGTAACGAA 
CTGCTAGCCG AATCGGATGT CTCCTGTCCC GAGGATGTGT TCATCCAGCA AGCGCTGGTG 
ATGCTTCAGG AGCGTGTTGA GAAGTTGGGT GTCGAGAAAC CTGATCCGAA AGAGGATGAA 
GAGGAGACAG CACCTGTACT TTGCCTAGAA ACCGAGACCA AGATTGATAT TGAGAAGTCA 
GACGATGCCT CCATCTCGTC GGGAGAAGCA TCCAGCAGCC TTAGCTACTC GAGCAACCAT 
AATAAATACT ATTATTTCTA CCAGTCAAAC GACGGACAGA ATATTTACTT GCATCCCTTG 
AACGTGAAGA TGCTTCAGGC TTGCTACGGC ACCTTGGACT TGGGTCCGCT ACTCATCGAA 
GCCCAAATCG TGCAGATGGA ACAGCACTCT ATGGATGAGG AGCATCGACG CAAGTTCACT 
TGTCTTGGCC ACTTGCCTTT GACTTGTCAG TTTTCGGTTG TGGAAGTCGA ATTGCAGCCG 
CCAGTGGTCA CTGGAGGAAT ACTCAAGCTC TTTAAGGAGG ACATTCTTCA TCGTAAGAAG 
GAACGACAGC GTCGGGATCG CGAGGAGCGC AAGCGAGAGC AGCACATCAA CGAGATAAAT 
GACCGCCAGA TGGGCAAACT TATTGCCAGT GCAGCTAACC TTGACCTAAG CTCCTCCCAT 
GAGTTTCCCA CTTGCGGCTT CGAGGAGGCT TTGCCCGCTC CCAGTGGCTC TGTGCCCATG 
AACATCAGCA ACCAGGACCC CGGTTCCCGA TACTCCAGTG TCACTCTGGG AAGTCCCAAG 
CAGGAGATGT GGCCCACTAT TGGAAGTAAT TCTCCTGGAG GTGGTGGCGC GCCATTGGAT 
GTTCAAGTGG GCGCCTGGGG CCGTTCAGCT CCACCGCCGC CACGGGCTGT AATGGTCCCA 
AGAACCAGCG ATGAGGACTT TGAGCAGCGA TCTGTGGGCC ACTGGGGTCT GGGAGAACTT 
TTAGTTGGAG CCTTGGACCA GAAAAAGCAA AAGGTAGGAG CAGCAAAATC CAATGGAGCA 
GCTCCAGCGG AATCGAAGAA GCAAAAGAAG GCCAAGGGCA AGAAGATGGT TCCCCTGTTC 
GCCACGGGCA TGAACAGAGC TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_647623.1
CDS	603..2729
Translation	MDSNNSSNSKKQPQSSHHGKSSSAESQRKSSELHTNSNKQRHSRRRNQVPTPRNDQQQQPQQPAKLRPNVDKRPKARGFGGGYDFPSPRGQDGASGSSRLAYSATGYSRSGDFDHELNSVYTQGSKKQNLNHLLNFHCVPRELERGHHHQAQQHHGLSGRKQRYNKEQFLQANFQFVIRSGAKAQVNGSPDALIDWSYIEQINIQTTEELQCPICLYPPVAAKLTRCGHAYCWPCLLHYLSLSDKTWRKCPICYDAIHAGDLKSCTIEQLRDLQVGEKITFQLMRRRKGSMYIENHVAGLGETIERFPFVSAGEEAKRYSKFLIAKRMDVAAIIERERNELLAESDVSCPEDVFIQQALVMLQERVEKLGVEKPDPKEDEEETAPVLCLETETKIDIEKSDDASISSGEASSSLSYSSNHNKYYYFYQSNDGQNIYLHPLNVKMLQACYGTLDLGPLLIEAQIVQMEQHSMDEEHRRKFTCLGHLPLTCQFSVVEVELQPPVVTGGILKLFKEDILHRKKERQRRDREERKREQHINEINDRQMGKLIASAANLDLSSSHEFPTCGFEEALPAPSGSVPMNISNQDPGSRYSSVTLGSPKQEMWPTIGSNSPGGGGAPLDVQVGAWGRSAPPPPRAVMVPRTSDEDFEQRSVGHWGLGELLVGALDQKKQKVGAAKSNGAAPAESKKQKKAKGKKMVPLFATGMNRAP

Target ORF information:

RefSeq Version	NM_139366.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG12099), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139366.3

ORF Insert Sequence:

ATGGACAGCA ACAACAGCAG CAACAGCAAG AAGCAGCCTC AATCCTCTCA TCATGGCAAG 
TCCTCGTCTG CTGAATCGCA AAGGAAGTCC AGTGAGTTAC ATACCAACAG CAACAAGCAG 
CGACACAGCC GTCGAAGGAA TCAGGTTCCC ACACCGCGCA ACGATCAGCA ACAACAGCCA 
CAGCAGCCAG CAAAGCTACG TCCCAATGTG GACAAGCGTC CGAAGGCTCG CGGGTTTGGA 
GGCGGTTACG ATTTCCCTTC ACCCCGTGGC CAGGACGGGG CTTCAGGCAG CTCCCGACTA 
GCCTACTCCG CCACCGGCTA CTCACGTAGC GGTGACTTTG ACCATGAACT GAACTCTGTG 
TATACACAGG GCAGCAAGAA GCAGAATCTG AACCATCTGC TTAACTTCCA CTGCGTGCCC 
AGGGAACTGG AGCGAGGACA TCACCATCAG GCCCAACAGC ACCACGGCCT GAGTGGCCGA 
AAGCAGCGCT ACAACAAGGA GCAGTTCCTG CAGGCCAACT TTCAGTTCGT CATTAGATCG 
GGTGCAAAGG CTCAGGTTAA TGGCTCGCCG GATGCACTCA TCGATTGGAG CTACATCGAG 
CAGATCAACA TACAGACCAC TGAGGAGTTG CAGTGCCCCA TCTGCCTGTA CCCGCCTGTG 
GCTGCCAAGC TCACCAGATG CGGACACGCC TACTGTTGGC CCTGCTTGCT GCACTACCTC 
TCGCTGAGCG ACAAGACCTG GCGCAAGTGT CCTATCTGCT ATGACGCCAT CCACGCGGGA 
GATCTGAAGA GCTGCACCAT CGAACAGCTG AGGGATTTGC AAGTGGGCGA GAAGATTACC 
TTCCAGTTGA TGCGGCGTCG CAAGGGCTCC ATGTACATTG AAAATCATGT TGCAGGATTG 
GGCGAAACAA TTGAACGTTT CCCCTTTGTG TCCGCTGGTG AAGAGGCTAA ACGCTACTCA 
AAGTTTCTAA TAGCCAAGCG GATGGATGTG GCCGCCATCA TCGAGAGGGA GCGTAACGAA 
CTGCTAGCCG AATCGGATGT CTCCTGTCCC GAGGATGTGT TCATCCAGCA AGCGCTGGTG 
ATGCTTCAGG AGCGTGTTGA GAAGTTGGGT GTCGAGAAAC CTGATCCGAA AGAGGATGAA 
GAGGAGACAG CACCTGTACT TTGCCTAGAA ACCGAGACCA AGATTGATAT TGAGAAGTCA 
GACGATGCCT CCATCTCGTC GGGAGAAGCA TCCAGCAGCC TTAGCTACTC GAGCAACCAT 
AATAAATACT ATTATTTCTA CCAGTCAAAC GACGGACAGA ATATTTACTT GCATCCCTTG 
AACGTGAAGA TGCTTCAGGC TTGCTACGGC ACCTTGGACT TGGGTCCGCT ACTCATCGAA 
GCCCAAATCG TGCAGATGGA ACAGCACTCT ATGGATGAGG AGCATCGACG CAAGTTCACT 
TGTCTTGGCC ACTTGCCTTT GACTTGTCAG TTTTCGGTTG TGGAAGTCGA ATTGCAGCCG 
CCAGTGGTCA CTGGAGGAAT ACTCAAGCTC TTTAAGGAGG ACATTCTTCA TCGTAAGAAG 
GAACGACAGC GTCGGGATCG CGAGGAGCGC AAGCGAGAGC AGCACATCAA CGAGATAAAT 
GACCGCCAGA TGGGCAAACT TATTGCCAGT GCAGCTAACC TTGACCTAAG CTCCTCCCAT 
GAGTTTCCCA CTTGCGGCTT CGAGGAGGCT TTGCCCGCTC CCAGTGGCTC TGTGCCCATG 
AACATCAGCA ACCAGGACCC CGGTTCCCGA TACTCCAGTG TCACTCTGGG AAGTCCCAAG 
CAGGAGATGT GGCCCACTAT TGGAAGTAAT TCTCCTGGAG GTGGTGGCGC GCCATTGGAT 
GTTCAAGTGG GCGCCTGGGG CCGTTCAGCT CCACCGCCGC CACGGGCTGT AATGGTCCCA 
AGAACCAGCG ATGAGGACTT TGAGCAGCGA TCTGTGGGCC ACTGGGGTCT GGGAGAACTT 
TTAGTTGGAG CCTTGGACCA GAAAAAGCAA AAGGTAGGAG CAGCAAAATC CAATGGAGCA 
GCTCCAGCGG AATCGAAGAA GCAAAAGAAG GCCAAGGGCA AGAAGATGGT TCCCCTGTTC 
GCCACGGGCA TGAACAGAGC TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG12099 ( NM_167895.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728629.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG12099), mRNA.

Price & Availability↑

CloneID	OFa17174
Clone ID Related Accession (Same CDS sequence)	NM_139366.3 , NM_167895.2
Accession Version	NM_167895.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_167895.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACAGCA ACAACAGCAG CAACAGCAAG AAGCAGCCTC AATCCTCTCA TCATGGCAAG 
TCCTCGTCTG CTGAATCGCA AAGGAAGTCC AGTGAGTTAC ATACCAACAG CAACAAGCAG 
CGACACAGCC GTCGAAGGAA TCAGGTTCCC ACACCGCGCA ACGATCAGCA ACAACAGCCA 
CAGCAGCCAG CAAAGCTACG TCCCAATGTG GACAAGCGTC CGAAGGCTCG CGGGTTTGGA 
GGCGGTTACG ATTTCCCTTC ACCCCGTGGC CAGGACGGGG CTTCAGGCAG CTCCCGACTA 
GCCTACTCCG CCACCGGCTA CTCACGTAGC GGTGACTTTG ACCATGAACT GAACTCTGTG 
TATACACAGG GCAGCAAGAA GCAGAATCTG AACCATCTGC TTAACTTCCA CTGCGTGCCC 
AGGGAACTGG AGCGAGGACA TCACCATCAG GCCCAACAGC ACCACGGCCT GAGTGGCCGA 
AAGCAGCGCT ACAACAAGGA GCAGTTCCTG CAGGCCAACT TTCAGTTCGT CATTAGATCG 
GGTGCAAAGG CTCAGGTTAA TGGCTCGCCG GATGCACTCA TCGATTGGAG CTACATCGAG 
CAGATCAACA TACAGACCAC TGAGGAGTTG CAGTGCCCCA TCTGCCTGTA CCCGCCTGTG 
GCTGCCAAGC TCACCAGATG CGGACACGCC TACTGTTGGC CCTGCTTGCT GCACTACCTC 
TCGCTGAGCG ACAAGACCTG GCGCAAGTGT CCTATCTGCT ATGACGCCAT CCACGCGGGA 
GATCTGAAGA GCTGCACCAT CGAACAGCTG AGGGATTTGC AAGTGGGCGA GAAGATTACC 
TTCCAGTTGA TGCGGCGTCG CAAGGGCTCC ATGTACATTG AAAATCATGT TGCAGGATTG 
GGCGAAACAA TTGAACGTTT CCCCTTTGTG TCCGCTGGTG AAGAGGCTAA ACGCTACTCA 
AAGTTTCTAA TAGCCAAGCG GATGGATGTG GCCGCCATCA TCGAGAGGGA GCGTAACGAA 
CTGCTAGCCG AATCGGATGT CTCCTGTCCC GAGGATGTGT TCATCCAGCA AGCGCTGGTG 
ATGCTTCAGG AGCGTGTTGA GAAGTTGGGT GTCGAGAAAC CTGATCCGAA AGAGGATGAA 
GAGGAGACAG CACCTGTACT TTGCCTAGAA ACCGAGACCA AGATTGATAT TGAGAAGTCA 
GACGATGCCT CCATCTCGTC GGGAGAAGCA TCCAGCAGCC TTAGCTACTC GAGCAACCAT 
AATAAATACT ATTATTTCTA CCAGTCAAAC GACGGACAGA ATATTTACTT GCATCCCTTG 
AACGTGAAGA TGCTTCAGGC TTGCTACGGC ACCTTGGACT TGGGTCCGCT ACTCATCGAA 
GCCCAAATCG TGCAGATGGA ACAGCACTCT ATGGATGAGG AGCATCGACG CAAGTTCACT 
TGTCTTGGCC ACTTGCCTTT GACTTGTCAG TTTTCGGTTG TGGAAGTCGA ATTGCAGCCG 
CCAGTGGTCA CTGGAGGAAT ACTCAAGCTC TTTAAGGAGG ACATTCTTCA TCGTAAGAAG 
GAACGACAGC GTCGGGATCG CGAGGAGCGC AAGCGAGAGC AGCACATCAA CGAGATAAAT 
GACCGCCAGA TGGGCAAACT TATTGCCAGT GCAGCTAACC TTGACCTAAG CTCCTCCCAT 
GAGTTTCCCA CTTGCGGCTT CGAGGAGGCT TTGCCCGCTC CCAGTGGCTC TGTGCCCATG 
AACATCAGCA ACCAGGACCC CGGTTCCCGA TACTCCAGTG TCACTCTGGG AAGTCCCAAG 
CAGGAGATGT GGCCCACTAT TGGAAGTAAT TCTCCTGGAG GTGGTGGCGC GCCATTGGAT 
GTTCAAGTGG GCGCCTGGGG CCGTTCAGCT CCACCGCCGC CACGGGCTGT AATGGTCCCA 
AGAACCAGCG ATGAGGACTT TGAGCAGCGA TCTGTGGGCC ACTGGGGTCT GGGAGAACTT 
TTAGTTGGAG CCTTGGACCA GAAAAAGCAA AAGGTAGGAG CAGCAAAATC CAATGGAGCA 
GCTCCAGCGG AATCGAAGAA GCAAAAGAAG GCCAAGGGCA AGAAGATGGT TCCCCTGTTC 
GCCACGGGCA TGAACAGAGC TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728629.1
CDS	495..2621
Translation	MDSNNSSNSKKQPQSSHHGKSSSAESQRKSSELHTNSNKQRHSRRRNQVPTPRNDQQQQPQQPAKLRPNVDKRPKARGFGGGYDFPSPRGQDGASGSSRLAYSATGYSRSGDFDHELNSVYTQGSKKQNLNHLLNFHCVPRELERGHHHQAQQHHGLSGRKQRYNKEQFLQANFQFVIRSGAKAQVNGSPDALIDWSYIEQINIQTTEELQCPICLYPPVAAKLTRCGHAYCWPCLLHYLSLSDKTWRKCPICYDAIHAGDLKSCTIEQLRDLQVGEKITFQLMRRRKGSMYIENHVAGLGETIERFPFVSAGEEAKRYSKFLIAKRMDVAAIIERERNELLAESDVSCPEDVFIQQALVMLQERVEKLGVEKPDPKEDEEETAPVLCLETETKIDIEKSDDASISSGEASSSLSYSSNHNKYYYFYQSNDGQNIYLHPLNVKMLQACYGTLDLGPLLIEAQIVQMEQHSMDEEHRRKFTCLGHLPLTCQFSVVEVELQPPVVTGGILKLFKEDILHRKKERQRRDREERKREQHINEINDRQMGKLIASAANLDLSSSHEFPTCGFEEALPAPSGSVPMNISNQDPGSRYSSVTLGSPKQEMWPTIGSNSPGGGGAPLDVQVGAWGRSAPPPPRAVMVPRTSDEDFEQRSVGHWGLGELLVGALDQKKQKVGAAKSNGAAPAESKKQKKAKGKKMVPLFATGMNRAP

Target ORF information:

RefSeq Version	NM_167895.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG12099), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167895.2

ORF Insert Sequence:

ATGGACAGCA ACAACAGCAG CAACAGCAAG AAGCAGCCTC AATCCTCTCA TCATGGCAAG 
TCCTCGTCTG CTGAATCGCA AAGGAAGTCC AGTGAGTTAC ATACCAACAG CAACAAGCAG 
CGACACAGCC GTCGAAGGAA TCAGGTTCCC ACACCGCGCA ACGATCAGCA ACAACAGCCA 
CAGCAGCCAG CAAAGCTACG TCCCAATGTG GACAAGCGTC CGAAGGCTCG CGGGTTTGGA 
GGCGGTTACG ATTTCCCTTC ACCCCGTGGC CAGGACGGGG CTTCAGGCAG CTCCCGACTA 
GCCTACTCCG CCACCGGCTA CTCACGTAGC GGTGACTTTG ACCATGAACT GAACTCTGTG 
TATACACAGG GCAGCAAGAA GCAGAATCTG AACCATCTGC TTAACTTCCA CTGCGTGCCC 
AGGGAACTGG AGCGAGGACA TCACCATCAG GCCCAACAGC ACCACGGCCT GAGTGGCCGA 
AAGCAGCGCT ACAACAAGGA GCAGTTCCTG CAGGCCAACT TTCAGTTCGT CATTAGATCG 
GGTGCAAAGG CTCAGGTTAA TGGCTCGCCG GATGCACTCA TCGATTGGAG CTACATCGAG 
CAGATCAACA TACAGACCAC TGAGGAGTTG CAGTGCCCCA TCTGCCTGTA CCCGCCTGTG 
GCTGCCAAGC TCACCAGATG CGGACACGCC TACTGTTGGC CCTGCTTGCT GCACTACCTC 
TCGCTGAGCG ACAAGACCTG GCGCAAGTGT CCTATCTGCT ATGACGCCAT CCACGCGGGA 
GATCTGAAGA GCTGCACCAT CGAACAGCTG AGGGATTTGC AAGTGGGCGA GAAGATTACC 
TTCCAGTTGA TGCGGCGTCG CAAGGGCTCC ATGTACATTG AAAATCATGT TGCAGGATTG 
GGCGAAACAA TTGAACGTTT CCCCTTTGTG TCCGCTGGTG AAGAGGCTAA ACGCTACTCA 
AAGTTTCTAA TAGCCAAGCG GATGGATGTG GCCGCCATCA TCGAGAGGGA GCGTAACGAA 
CTGCTAGCCG AATCGGATGT CTCCTGTCCC GAGGATGTGT TCATCCAGCA AGCGCTGGTG 
ATGCTTCAGG AGCGTGTTGA GAAGTTGGGT GTCGAGAAAC CTGATCCGAA AGAGGATGAA 
GAGGAGACAG CACCTGTACT TTGCCTAGAA ACCGAGACCA AGATTGATAT TGAGAAGTCA 
GACGATGCCT CCATCTCGTC GGGAGAAGCA TCCAGCAGCC TTAGCTACTC GAGCAACCAT 
AATAAATACT ATTATTTCTA CCAGTCAAAC GACGGACAGA ATATTTACTT GCATCCCTTG 
AACGTGAAGA TGCTTCAGGC TTGCTACGGC ACCTTGGACT TGGGTCCGCT ACTCATCGAA 
GCCCAAATCG TGCAGATGGA ACAGCACTCT ATGGATGAGG AGCATCGACG CAAGTTCACT 
TGTCTTGGCC ACTTGCCTTT GACTTGTCAG TTTTCGGTTG TGGAAGTCGA ATTGCAGCCG 
CCAGTGGTCA CTGGAGGAAT ACTCAAGCTC TTTAAGGAGG ACATTCTTCA TCGTAAGAAG 
GAACGACAGC GTCGGGATCG CGAGGAGCGC AAGCGAGAGC AGCACATCAA CGAGATAAAT 
GACCGCCAGA TGGGCAAACT TATTGCCAGT GCAGCTAACC TTGACCTAAG CTCCTCCCAT 
GAGTTTCCCA CTTGCGGCTT CGAGGAGGCT TTGCCCGCTC CCAGTGGCTC TGTGCCCATG 
AACATCAGCA ACCAGGACCC CGGTTCCCGA TACTCCAGTG TCACTCTGGG AAGTCCCAAG 
CAGGAGATGT GGCCCACTAT TGGAAGTAAT TCTCCTGGAG GTGGTGGCGC GCCATTGGAT 
GTTCAAGTGG GCGCCTGGGG CCGTTCAGCT CCACCGCCGC CACGGGCTGT AATGGTCCCA 
AGAACCAGCG ATGAGGACTT TGAGCAGCGA TCTGTGGGCC ACTGGGGTCT GGGAGAACTT 
TTAGTTGGAG CCTTGGACCA GAAAAAGCAA AAGGTAGGAG CAGCAAAATC CAATGGAGCA 
GCTCCAGCGG AATCGAAGAA GCAAAAGAAG GCCAAGGGCA AGAAGATGGT TCCCCTGTTC 
GCCACGGGCA TGAACAGAGC TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

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CG12099 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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