stet cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	stet
Entrez Gene ID	38169
Full Name	stem cell tumor
Synonyms	B-Rho,Brho,CG12083,CG12504,CG33166,Dmel\CG33166,Rho-2,Rho2,brho,rho-2,rho2
General protein information	Preferred Names stem cell tumor Names CG33166 gene product from transcript CG33166-RB CG33166-PA CG33166-PB brother of rhomboid rhomboid-2 stet-PA stet-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 62A2-62A2

mRNA and Protein(s)

mRNA	Protein	Name
NM_176273.2	NP_788451.1	stem cell tumor, isoform A
NM_176272.2	NP_788450.1	stem cell tumor, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	RHBDL3	NP_612201.1
Pan troglodytes (chimpanzee)	RHBDL3	XP_001158831.2
	RHBDL3	XP_001112770.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005058	XP_313932.4
Bos taurus (cattle)	RHBDL3	XP_002695704.2
Mus musculus (house mouse)	Rhbdl3	NP_631974.1
Danio rerio (zebrafish)	rhbdl3	NP_001017556.1
Drosophila melanogaster (fruit fly)	stet	NP_788450.1
Xenopus tropicalis (tropical clawed frog)	LOC101732375	XP_004920771.1
Canis lupus familiaris (dog)	RHBDL3	XP_005624882.1
Rattus norvegicus (Norway rat)	Rhbdl3	NP_001099289.1
Gallus gallus (chicken)	RHBDL3	XP_004946238.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rho-2, which is expressed in the germ line, cleaves the Spitz precursor and Star already in the endoplasmic reticulum, in addition to its activity in the late compartment.
Title: Drosophila EGFR signalling is modulated by differential compartmentalization of Rhomboid intramembrane proteases.

The following stet gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the stet cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17158	NM_176272.2 Latest version!	Drosophila melanogaster stem cell tumor (stet), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa17159	NM_176273.2 Latest version!	Drosophila melanogaster stem cell tumor (stet), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

stet ( NM_176272.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788450.1 Drosophila melanogaster stem cell tumor (stet), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa17158
Clone ID Related Accession (Same CDS sequence)	NM_176272.2
Accession Version	NM_176272.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	stem cell tumor, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_176272.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGCCCG GCTCCGAGCG AGGAAGCTCG CGGGCCCTGG AAATCCCAAC CAGCCAGCAA 
AATGCCGGCG GTAGTAGTCA GCAGGAGACT CTGGCCGACA TCGAGCACAT ATCCACCATT 
GCCGGCTCCC TTGCCTCCAT TGGATCCCTC TCGCACACCC AAATGCGCTA TCAATGCACC 
AATGATGCCG GCTATGATAC GGAACACACG TCCCTGAACT CGGACTTCGA TGAGGCCGAG 
CTGCGACGGG AGCTACTAAG AGATAAATGG AAACTGCTCT TTGACATGTT CGATCCCGAG 
GGTTTTGGCG AAATATCAGT CGAGGAATTC CTGGAGGCCC TCAAGTCGCC GGAGTTCCTG 
TCCCAAGTGC CTATGAACAA GCGGGAGCTG CTGCTGGAGC GGGCAAAGAA GGCGAAGCTG 
CCCACAGGAC CCGGCTACGT GACGTTCCAG GACTTTGTGA ATGTGATGAG CGGCAAGCGG 
ACACGTAGCT TTAAGTGCGC CGTACACCAT CGAGATCGTG AAGTCTGCTC CGAAAACGAT 
TTCCAGCTGG TGCTAAACGA ACCGCCACTC TTTCGCAAAA TGGTGCACGC CGTGGCCATG 
GAGATCCTGC CCGAGGAGCG GGATCGCAAG TACTACGCCG ATCGCTACAC CTGCTGTCCG 
CCGCCTTTTT TCATCATCCT GGTTACTCTG GTGGAGCTGG GTTTCTTTGT TTACCACTCA 
GTGGTCACGG GTGAGGCGGC GCCAAGGGGA CCCATACCCT CGGACTCCAT GTTCATCTAC 
CGTCCCGATA AGCGTCACGA GATCTGGCGC TTCCTCTTCT ACATGGTACT CCATGCCGGG 
TGGCTGCATC TCGGTTTTAA CGTGGCAGTT CAGCTGGTTT TTGGGCTGCC ACTGGAGATG 
GTTCATGGTT CCACGAGGAT AGCCTGCATC TACTTCTCCG GGGTGCTGGC GGGAAGCCTG 
GGCACTAGCA TCTTTGACCC GGATGTGTTC CTTGTGGGCG CCAGTGGTGG AGTATACGCT 
TTGCTAGCCG CCCACCTGGC CAATGTGCTT CTCAACTATC ACCAGATGCG TTATGGAGTC 
ATCAAGCTGC TTCACATTCT GGTCTTCGTT TCCTTCGACT TTGGCTTTGC CATCTATGCT 
CGCTATGCTG GCGACGAACT GCAGCTGGGA TCATCGAGTG AATTCCTGGC CATAGATCAG 
GCGGAAACGG CGGGTGCTGT TTCGTATGTG GCCCATTTGG CTGGTGCCAT TGCTGGCCTA 
ACTATCGGGC TGCTGGTGCT CAAGAGCTTT GAGCAGAAGC TCCACGAGCA GCTGCTCTGG 
TGGATCGCCT TGGGAACGTA CTTGGCGCTG GTGGTCTTCG CCATCGCCTT CAACATCATG 
AATGGATTTG CGATGTTCAA TATACGCGTG GAGAAGATCC GTGTCACGGA AACCATTTTC 
AATGATTTCC AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788450.1
CDS	188..1645
Translation	MWPGSERGSSRALEIPTSQQNAGGSSQQETLADIEHISTIAGSLASIGSLSHTQMRYQCTNDAGYDTEHTSLNSDFDEAELRRELLRDKWKLLFDMFDPEGFGEISVEEFLEALKSPEFLSQVPMNKRELLLERAKKAKLPTGPGYVTFQDFVNVMSGKRTRSFKCAVHHRDREVCSENDFQLVLNEPPLFRKMVHAVAMEILPEERDRKYYADRYTCCPPPFFIILVTLVELGFFVYHSVVTGEAAPRGPIPSDSMFIYRPDKRHEIWRFLFYMVLHAGWLHLGFNVAVQLVFGLPLEMVHGSTRIACIYFSGVLAGSLGTSIFDPDVFLVGASGGVYALLAAHLANVLLNYHQMRYGVIKLLHILVFVSFDFGFAIYARYAGDELQLGSSSEFLAIDQAETAGAVSYVAHLAGAIAGLTIGLLVLKSFEQKLHEQLLWWIALGTYLALVVFAIAFNIMNGFAMFNIRVEKIRVTETIFNDFQV

Target ORF information:

RefSeq Version	NM_176272.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster stem cell tumor (stet), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176272.2

ORF Insert Sequence:

ATGTGGCCCG GCTCCGAGCG AGGAAGCTCG CGGGCCCTGG AAATCCCAAC CAGCCAGCAA 
AATGCCGGCG GTAGTAGTCA GCAGGAGACT CTGGCCGACA TCGAGCACAT ATCCACCATT 
GCCGGCTCCC TTGCCTCCAT TGGATCCCTC TCGCACACCC AAATGCGCTA TCAATGCACC 
AATGATGCCG GCTATGATAC GGAACACACG TCCCTGAACT CGGACTTCGA TGAGGCCGAG 
CTGCGACGGG AGCTACTAAG AGATAAATGG AAACTGCTCT TTGACATGTT CGATCCCGAG 
GGTTTTGGCG AAATATCAGT CGAGGAATTC CTGGAGGCCC TCAAGTCGCC GGAGTTCCTG 
TCCCAAGTGC CTATGAACAA GCGGGAGCTG CTGCTGGAGC GGGCAAAGAA GGCGAAGCTG 
CCCACAGGAC CCGGCTACGT GACGTTCCAG GACTTTGTGA ATGTGATGAG CGGCAAGCGG 
ACACGTAGCT TTAAGTGCGC CGTACACCAT CGAGATCGTG AAGTCTGCTC CGAAAACGAT 
TTCCAGCTGG TGCTAAACGA ACCGCCACTC TTTCGCAAAA TGGTGCACGC CGTGGCCATG 
GAGATCCTGC CCGAGGAGCG GGATCGCAAG TACTACGCCG ATCGCTACAC CTGCTGTCCG 
CCGCCTTTTT TCATCATCCT GGTTACTCTG GTGGAGCTGG GTTTCTTTGT TTACCACTCA 
GTGGTCACGG GTGAGGCGGC GCCAAGGGGA CCCATACCCT CGGACTCCAT GTTCATCTAC 
CGTCCCGATA AGCGTCACGA GATCTGGCGC TTCCTCTTCT ACATGGTACT CCATGCCGGG 
TGGCTGCATC TCGGTTTTAA CGTGGCAGTT CAGCTGGTTT TTGGGCTGCC ACTGGAGATG 
GTTCATGGTT CCACGAGGAT AGCCTGCATC TACTTCTCCG GGGTGCTGGC GGGAAGCCTG 
GGCACTAGCA TCTTTGACCC GGATGTGTTC CTTGTGGGCG CCAGTGGTGG AGTATACGCT 
TTGCTAGCCG CCCACCTGGC CAATGTGCTT CTCAACTATC ACCAGATGCG TTATGGAGTC 
ATCAAGCTGC TTCACATTCT GGTCTTCGTT TCCTTCGACT TTGGCTTTGC CATCTATGCT 
CGCTATGCTG GCGACGAACT GCAGCTGGGA TCATCGAGTG AATTCCTGGC CATAGATCAG 
GCGGAAACGG CGGGTGCTGT TTCGTATGTG GCCCATTTGG CTGGTGCCAT TGCTGGCCTA 
ACTATCGGGC TGCTGGTGCT CAAGAGCTTT GAGCAGAAGC TCCACGAGCA GCTGCTCTGG 
TGGATCGCCT TGGGAACGTA CTTGGCGCTG GTGGTCTTCG CCATCGCCTT CAACATCATG 
AATGGATTTG CGATGTTCAA TATACGCGTG GAGAAGATCC GTGTCACGGA AACCATTTTC 
AATGATTTCC AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

stet ( NM_176273.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788451.1 Drosophila melanogaster stem cell tumor (stet), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa17159
Clone ID Related Accession (Same CDS sequence)	NM_176273.2
Accession Version	NM_176273.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	stem cell tumor, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_176273.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGGCA AGCGGACACG TAGCTTTAAG TGCGCCGTAC ACCATCGAGA TCGTGAAGTC 
TGCTCCGAAA ACGATTTCCA GCTGGTGCTA AACGAACCGC CACTCTTTCG CAAAATGGTG 
CACGCCGTGG CCATGGAGAT CCTGCCCGAG GAGCGGGATC GCAAGTACTA CGCCGATCGC 
TACACCTGCT GTCCGCCGCC TTTTTTCATC ATCCTGGTTA CTCTGGTGGA GCTGGGTTTC 
TTTGTTTACC ACTCAGTGGT CACGGGTGAG GCGGCGCCAA GGGGACCCAT ACCCTCGGAC 
TCCATGTTCA TCTACCGTCC CGATAAGCGT CACGAGATCT GGCGCTTCCT CTTCTACATG 
GTACTCCATG CCGGGTGGCT GCATCTCGGT TTTAACGTGG CAGTTCAGCT GGTTTTTGGG 
CTGCCACTGG AGATGGTTCA TGGTTCCACG AGGATAGCCT GCATCTACTT CTCCGGGGTG 
CTGGCGGGAA GCCTGGGCAC TAGCATCTTT GACCCGGATG TGTTCCTTGT GGGCGCCAGT 
GGTGGAGTAT ACGCTTTGCT AGCCGCCCAC CTGGCCAATG TGCTTCTCAA CTATCACCAG 
ATGCGTTATG GAGTCATCAA GCTGCTTCAC ATTCTGGTCT TCGTTTCCTT CGACTTTGGC 
TTTGCCATCT ATGCTCGCTA TGCTGGCGAC GAACTGCAGC TGGGATCATC GAGTGAATTC 
CTGGCCATAG ATCAGGCGGA AACGGCGGGT GCTGTTTCGT ATGTGGCCCA TTTGGCTGGT 
GCCATTGCTG GCCTAACTAT CGGGCTGCTG GTGCTCAAGA GCTTTGAGCA GAAGCTCCAC 
GAGCAGCTGC TCTGGTGGAT CGCCTTGGGA ACGTACTTGG CGCTGGTGGT CTTCGCCATC 
GCCTTCAACA TCATGAATGG ATTTGCGATG TTCAATATAC GCGTGGAGAA GATCCGTGTC 
ACGGAAACCA TTTTCAATGA TTTCCAGGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788451.1
CDS	171..1163
Translation	MSGKRTRSFKCAVHHRDREVCSENDFQLVLNEPPLFRKMVHAVAMEILPEERDRKYYADRYTCCPPPFFIILVTLVELGFFVYHSVVTGEAAPRGPIPSDSMFIYRPDKRHEIWRFLFYMVLHAGWLHLGFNVAVQLVFGLPLEMVHGSTRIACIYFSGVLAGSLGTSIFDPDVFLVGASGGVYALLAAHLANVLLNYHQMRYGVIKLLHILVFVSFDFGFAIYARYAGDELQLGSSSEFLAIDQAETAGAVSYVAHLAGAIAGLTIGLLVLKSFEQKLHEQLLWWIALGTYLALVVFAIAFNIMNGFAMFNIRVEKIRVTETIFNDFQV

Target ORF information:

RefSeq Version	NM_176273.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster stem cell tumor (stet), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176273.2

ORF Insert Sequence:

ATGAGCGGCA AGCGGACACG TAGCTTTAAG TGCGCCGTAC ACCATCGAGA TCGTGAAGTC 
TGCTCCGAAA ACGATTTCCA GCTGGTGCTA AACGAACCGC CACTCTTTCG CAAAATGGTG 
CACGCCGTGG CCATGGAGAT CCTGCCCGAG GAGCGGGATC GCAAGTACTA CGCCGATCGC 
TACACCTGCT GTCCGCCGCC TTTTTTCATC ATCCTGGTTA CTCTGGTGGA GCTGGGTTTC 
TTTGTTTACC ACTCAGTGGT CACGGGTGAG GCGGCGCCAA GGGGACCCAT ACCCTCGGAC 
TCCATGTTCA TCTACCGTCC CGATAAGCGT CACGAGATCT GGCGCTTCCT CTTCTACATG 
GTACTCCATG CCGGGTGGCT GCATCTCGGT TTTAACGTGG CAGTTCAGCT GGTTTTTGGG 
CTGCCACTGG AGATGGTTCA TGGTTCCACG AGGATAGCCT GCATCTACTT CTCCGGGGTG 
CTGGCGGGAA GCCTGGGCAC TAGCATCTTT GACCCGGATG TGTTCCTTGT GGGCGCCAGT 
GGTGGAGTAT ACGCTTTGCT AGCCGCCCAC CTGGCCAATG TGCTTCTCAA CTATCACCAG 
ATGCGTTATG GAGTCATCAA GCTGCTTCAC ATTCTGGTCT TCGTTTCCTT CGACTTTGGC 
TTTGCCATCT ATGCTCGCTA TGCTGGCGAC GAACTGCAGC TGGGATCATC GAGTGAATTC 
CTGGCCATAG ATCAGGCGGA AACGGCGGGT GCTGTTTCGT ATGTGGCCCA TTTGGCTGGT 
GCCATTGCTG GCCTAACTAT CGGGCTGCTG GTGCTCAAGA GCTTTGAGCA GAAGCTCCAC 
GAGCAGCTGC TCTGGTGGAT CGCCTTGGGA ACGTACTTGG CGCTGGTGGT CTTCGCCATC 
GCCTTCAACA TCATGAATGG ATTTGCGATG TTCAATATAC GCGTGGAGAA GATCCGTGTC 
ACGGAAACCA TTTTCAATGA TTTCCAGGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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stet cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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