rho cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	rho
Entrez Gene ID	38168
Full Name	rhomboid
Synonyms	CG1004,DMRHO,DMRHOa,DMRHOb,DRORHO,DmRho1,Dmel\CG1004,Dmel\rho,Dmrho,RHO,RHOb,Rho,Rho-1,Rho1,Ve,iks,rho-1,rho1,rhom,rhomboid/veinlet,ve
General protein information	Preferred Names rhomboid Names CG1004 gene product from transcript CG1004-RB CG1004-PA CG1004-PB rho-PA rho-PB rhomboid 1 rhomboid-1 rhomboid/veinlet rhomboid1 veinlet
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 62A2-62A2\|3-0.2 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_079159.3	NP_523883.2	rhomboid, isoform A
NM_001274326.1	NP_001261255.1	rhomboid, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Gro inhibits rho expression in undifferentiated cells and represses the expression of both ato and rho in non-R8 precursors during initiation of photoreceptor differentiation in an E(spl)-dependent manner.
Title: Groucho restricts rhomboid expression and couples EGFR activation with R8 selection during Drosophila photoreceptor differentiation.

a rhomboid enhancer element that selectively labels four Drosophila embryonic neural precursors, was characterized.
Title: Rhomboid Enhancer Activity Defines a Subset of Drosophila Neural Precursors Required for Proper Feeding, Growth and Viability.

Structure and mechanism of rhomboid protease.
Title: Structure and mechanism of rhomboid protease.

analysis of EGFR-dependent network interactions that pattern Drosophila eggshell appendages; each appendage is derived from a primordium comprising a patch of cells expressing broad (br) and an adjacent stripe of cells expressing rhomboid (rho)
Title: EGFR-dependent network interactions that pattern Drosophila eggshell appendages.

rhomboid may function in R8 cells to activate Epidermal growth factor receptor signaling in R7 cells
Title: rhomboid mediates specification of blue- and green-sensitive R8 photoreceptor cells in Drosophila.

The following rho gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rho cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17157	NM_001274326.1 Latest version!	Drosophila melanogaster rhomboid (rho), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa17157	NM_079159.3 Latest version!	Drosophila melanogaster rhomboid (rho), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rho ( NM_079159.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523883.2 Drosophila melanogaster rhomboid (rho), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa17157
Clone ID Related Accession (Same CDS sequence)	NM_079159.3 , NM_001274326.1
Accession Version	NM_079159.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rhomboid, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079159.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAACT TAACGCAGAA TGTAAACGAA ACCAAGGTGG ATTTGGGCCA GGAGAAGGAG 
AAGGAGGCGT CGCAGGAGGA GGAGCATGCC ACCGCCGCCA AGGAGACCAT CATTGACATT 
CCCGCCGCGT GCTCCAGTTC CTCCAACTCC TCGTCGTACG ACACCGATTG CAGCACGGCG 
AGCAGCACCT GCTGCACCCG CCAAGGCGAG CACATCTACA TGCAACGCGA GGCCATCCCG 
GCCACGCCAC TTCCGGAGTC GGAGGATATC GGCCTGCTGA AGTACGTCCA CCGCCAGCAC 
TGGCCCTGGT TCATCCTAGT GATCTCCATC ATTGAGATTG CCATCTTCGC CTACGACCGC 
TACACAATGC CCGCCCAGAA TTTCGGGCTA CCCGTTCCGA TTCCGTCGGA TTCGGTGCTG 
GTCTATCGGC CGGACCGGCG TCTGCAGGTG TGGCGCTTCT TTAGCTACAT GTTCCTGCAC 
GCCAACTGGT TCCACCTGGG CTTCAATATC GTCATCCAGC TGTTTTTTGG CATTCCCCTG 
GAGGTGATGC ACGGCACGGC CAGGATCGGC GTGATCTACA TGGCGGGCGT TTTTGCCGGA 
TCCCTGGGCA CCAGTGTCGT CGACTCGGAG GTCTTCCTGG TGGGCGCCAG CGGTGGCGTC 
TATGCCCTGT TGGCCGCACA TCTGGCCAAC ATTACACTGA ACTATGCGCA CATGAAGAGC 
GCATCCACGC AACTCGGATC CGTTGTCATC TTTGTCTCCT GCGATCTGGG CTATGCTCTC 
TACACCCAAT ACTTCGATGG AAGCGCCTTC GCCAAGGGTC CCCAGGTGTC GTACATTGCC 
CACCTGACGG GAGCCCTGGC AGGACTAACG ATCGGCTTTC TGGTGCTAAA GAACTTCGGT 
CACCGAGAGT ACGAGCAGCT CATCTGGTGG CTAGCGTTGG GCGTCTACTG TGCCTTCACC 
GTCTTCGCCA TCGTTTTCAA CCTGATCAAC ACGGTGACCG CTCAGCTGAT GGAGGAGCAG 
GGTGAGGTGA TTACCCAGCA TCTGTTGCAC GACCTGGGAG TGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523883.2
CDS	342..1409
Translation	MENLTQNVNETKVDLGQEKEKEASQEEEHATAAKETIIDIPAACSSSSNSSSYDTDCSTASSTCCTRQGEHIYMQREAIPATPLPESEDIGLLKYVHRQHWPWFILVISIIEIAIFAYDRYTMPAQNFGLPVPIPSDSVLVYRPDRRLQVWRFFSYMFLHANWFHLGFNIVIQLFFGIPLEVMHGTARIGVIYMAGVFAGSLGTSVVDSEVFLVGASGGVYALLAAHLANITLNYAHMKSASTQLGSVVIFVSCDLGYALYTQYFDGSAFAKGPQVSYIAHLTGALAGLTIGFLVLKNFGHREYEQLIWWLALGVYCAFTVFAIVFNLINTVTAQLMEEQGEVITQHLLHDLGVS

Target ORF information:

RefSeq Version	NM_079159.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rhomboid (rho), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079159.3

ORF Insert Sequence:

ATGGAGAACT TAACGCAGAA TGTAAACGAA ACCAAGGTGG ATTTGGGCCA GGAGAAGGAG 
AAGGAGGCGT CGCAGGAGGA GGAGCATGCC ACCGCCGCCA AGGAGACCAT CATTGACATT 
CCCGCCGCGT GCTCCAGTTC CTCCAACTCC TCGTCGTACG ACACCGATTG CAGCACGGCG 
AGCAGCACCT GCTGCACCCG CCAAGGCGAG CACATCTACA TGCAACGCGA GGCCATCCCG 
GCCACGCCAC TTCCGGAGTC GGAGGATATC GGCCTGCTGA AGTACGTCCA CCGCCAGCAC 
TGGCCCTGGT TCATCCTAGT GATCTCCATC ATTGAGATTG CCATCTTCGC CTACGACCGC 
TACACAATGC CCGCCCAGAA TTTCGGGCTA CCCGTTCCGA TTCCGTCGGA TTCGGTGCTG 
GTCTATCGGC CGGACCGGCG TCTGCAGGTG TGGCGCTTCT TTAGCTACAT GTTCCTGCAC 
GCCAACTGGT TCCACCTGGG CTTCAATATC GTCATCCAGC TGTTTTTTGG CATTCCCCTG 
GAGGTGATGC ACGGCACGGC CAGGATCGGC GTGATCTACA TGGCGGGCGT TTTTGCCGGA 
TCCCTGGGCA CCAGTGTCGT CGACTCGGAG GTCTTCCTGG TGGGCGCCAG CGGTGGCGTC 
TATGCCCTGT TGGCCGCACA TCTGGCCAAC ATTACACTGA ACTATGCGCA CATGAAGAGC 
GCATCCACGC AACTCGGATC CGTTGTCATC TTTGTCTCCT GCGATCTGGG CTATGCTCTC 
TACACCCAAT ACTTCGATGG AAGCGCCTTC GCCAAGGGTC CCCAGGTGTC GTACATTGCC 
CACCTGACGG GAGCCCTGGC AGGACTAACG ATCGGCTTTC TGGTGCTAAA GAACTTCGGT 
CACCGAGAGT ACGAGCAGCT CATCTGGTGG CTAGCGTTGG GCGTCTACTG TGCCTTCACC 
GTCTTCGCCA TCGTTTTCAA CCTGATCAAC ACGGTGACCG CTCAGCTGAT GGAGGAGCAG 
GGTGAGGTGA TTACCCAGCA TCTGTTGCAC GACCTGGGAG TGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rho ( NM_001274326.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261255.1 Drosophila melanogaster rhomboid (rho), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa17157
Clone ID Related Accession (Same CDS sequence)	NM_079159.3 , NM_001274326.1
Accession Version	NM_001274326.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rhomboid, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAACT TAACGCAGAA TGTAAACGAA ACCAAGGTGG ATTTGGGCCA GGAGAAGGAG 
AAGGAGGCGT CGCAGGAGGA GGAGCATGCC ACCGCCGCCA AGGAGACCAT CATTGACATT 
CCCGCCGCGT GCTCCAGTTC CTCCAACTCC TCGTCGTACG ACACCGATTG CAGCACGGCG 
AGCAGCACCT GCTGCACCCG CCAAGGCGAG CACATCTACA TGCAACGCGA GGCCATCCCG 
GCCACGCCAC TTCCGGAGTC GGAGGATATC GGCCTGCTGA AGTACGTCCA CCGCCAGCAC 
TGGCCCTGGT TCATCCTAGT GATCTCCATC ATTGAGATTG CCATCTTCGC CTACGACCGC 
TACACAATGC CCGCCCAGAA TTTCGGGCTA CCCGTTCCGA TTCCGTCGGA TTCGGTGCTG 
GTCTATCGGC CGGACCGGCG TCTGCAGGTG TGGCGCTTCT TTAGCTACAT GTTCCTGCAC 
GCCAACTGGT TCCACCTGGG CTTCAATATC GTCATCCAGC TGTTTTTTGG CATTCCCCTG 
GAGGTGATGC ACGGCACGGC CAGGATCGGC GTGATCTACA TGGCGGGCGT TTTTGCCGGA 
TCCCTGGGCA CCAGTGTCGT CGACTCGGAG GTCTTCCTGG TGGGCGCCAG CGGTGGCGTC 
TATGCCCTGT TGGCCGCACA TCTGGCCAAC ATTACACTGA ACTATGCGCA CATGAAGAGC 
GCATCCACGC AACTCGGATC CGTTGTCATC TTTGTCTCCT GCGATCTGGG CTATGCTCTC 
TACACCCAAT ACTTCGATGG AAGCGCCTTC GCCAAGGGTC CCCAGGTGTC GTACATTGCC 
CACCTGACGG GAGCCCTGGC AGGACTAACG ATCGGCTTTC TGGTGCTAAA GAACTTCGGT 
CACCGAGAGT ACGAGCAGCT CATCTGGTGG CTAGCGTTGG GCGTCTACTG TGCCTTCACC 
GTCTTCGCCA TCGTTTTCAA CCTGATCAAC ACGGTGACCG CTCAGCTGAT GGAGGAGCAG 
GGTGAGGTGA TTACCCAGCA TCTGTTGCAC GACCTGGGAG TGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261255.1
CDS	342..1409
Translation	MENLTQNVNETKVDLGQEKEKEASQEEEHATAAKETIIDIPAACSSSSNSSSYDTDCSTASSTCCTRQGEHIYMQREAIPATPLPESEDIGLLKYVHRQHWPWFILVISIIEIAIFAYDRYTMPAQNFGLPVPIPSDSVLVYRPDRRLQVWRFFSYMFLHANWFHLGFNIVIQLFFGIPLEVMHGTARIGVIYMAGVFAGSLGTSVVDSEVFLVGASGGVYALLAAHLANITLNYAHMKSASTQLGSVVIFVSCDLGYALYTQYFDGSAFAKGPQVSYIAHLTGALAGLTIGFLVLKNFGHREYEQLIWWLALGVYCAFTVFAIVFNLINTVTAQLMEEQGEVITQHLLHDLGVS

Target ORF information:

RefSeq Version	NM_001274326.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rhomboid (rho), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274326.1

ORF Insert Sequence:

ATGGAGAACT TAACGCAGAA TGTAAACGAA ACCAAGGTGG ATTTGGGCCA GGAGAAGGAG 
AAGGAGGCGT CGCAGGAGGA GGAGCATGCC ACCGCCGCCA AGGAGACCAT CATTGACATT 
CCCGCCGCGT GCTCCAGTTC CTCCAACTCC TCGTCGTACG ACACCGATTG CAGCACGGCG 
AGCAGCACCT GCTGCACCCG CCAAGGCGAG CACATCTACA TGCAACGCGA GGCCATCCCG 
GCCACGCCAC TTCCGGAGTC GGAGGATATC GGCCTGCTGA AGTACGTCCA CCGCCAGCAC 
TGGCCCTGGT TCATCCTAGT GATCTCCATC ATTGAGATTG CCATCTTCGC CTACGACCGC 
TACACAATGC CCGCCCAGAA TTTCGGGCTA CCCGTTCCGA TTCCGTCGGA TTCGGTGCTG 
GTCTATCGGC CGGACCGGCG TCTGCAGGTG TGGCGCTTCT TTAGCTACAT GTTCCTGCAC 
GCCAACTGGT TCCACCTGGG CTTCAATATC GTCATCCAGC TGTTTTTTGG CATTCCCCTG 
GAGGTGATGC ACGGCACGGC CAGGATCGGC GTGATCTACA TGGCGGGCGT TTTTGCCGGA 
TCCCTGGGCA CCAGTGTCGT CGACTCGGAG GTCTTCCTGG TGGGCGCCAG CGGTGGCGTC 
TATGCCCTGT TGGCCGCACA TCTGGCCAAC ATTACACTGA ACTATGCGCA CATGAAGAGC 
GCATCCACGC AACTCGGATC CGTTGTCATC TTTGTCTCCT GCGATCTGGG CTATGCTCTC 
TACACCCAAT ACTTCGATGG AAGCGCCTTC GCCAAGGGTC CCCAGGTGTC GTACATTGCC 
CACCTGACGG GAGCCCTGGC AGGACTAACG ATCGGCTTTC TGGTGCTAAA GAACTTCGGT 
CACCGAGAGT ACGAGCAGCT CATCTGGTGG CTAGCGTTGG GCGTCTACTG TGCCTTCACC 
GTCTTCGCCA TCGTTTTCAA CCTGATCAAC ACGGTGACCG CTCAGCTGAT GGAGGAGCAG 
GGTGAGGTGA TTACCCAGCA TCTGTTGCAC GACCTGGGAG TGTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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rho cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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