Herc4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Herc4
Entrez Gene ID	38151
Full Name	SUMO-related HECT domain and RCC repeat protein for Toll pathway activation
Synonyms	CG9153,Dmel\CG9153,FBgn0035207,NM_167868.1,sherpa
General protein information	Preferred Names SUMO-related HECT domain and RCC repeat protein for Toll pathway activation Names CG9153 gene product from transcript CG9153-RA CG9153-PA CG9153-PB CG9153-PC SUMO-related HECT (homologous to the E6-AP carboxyl terminus) domain and RCC (regulator of chromosome condensation) repeat protein for toll pathway activation SUMO-related HECT domain and RCC repeat protein for toll pathway activation Sherpa-PA Sherpa-PB Sherpa-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 61F6-61F6

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274320.1	NP_001261249.1	HECT and RLD domain containing E3 ubiquitin ligase 4, isoform C
NM_138254.3	NP_612098.2	HECT and RLD domain containing E3 ubiquitin ligase 4, isoform B
NM_167868.2	NP_728591.1	HECT and RLD domain containing E3 ubiquitin ligase 4, isoform A

Pathways from BioSystems

Homologies

Homo sapiens (human)	HERC4	NP_071362.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006994	XP_308776.4
Canis lupus familiaris (dog)	HERC4	XP_005618991.1
Danio rerio (zebrafish)	herc4	XP_685685.5
Drosophila melanogaster (fruit fly)	CG9153	NP_001261249.1
Bos taurus (cattle)	HERC4	NP_001070362.2
Pan troglodytes (chimpanzee)	HERC4	XP_001167753.1
Mus musculus (house mouse)	Herc4	NP_084390.1
Rattus norvegicus (Norway rat)	Herc4	NP_001012074.1
Gallus gallus (chicken)	HERC4	XP_004942077.1
Arabidopsis thaliana (thale cress)	UVR8	NP_201191.1
Oryza sativa (rice)	Os02g0554100	NP_001047115.1
Xenopus tropicalis (tropical clawed frog)	herc4	NP_001122122.1
	HERC4	XP_001087608.1
Oryza sativa (rice)	Os04g0435700	NP_001052849.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Herc4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Herc4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17135	NM_167868.2 Latest version!	Drosophila melanogaster HECT and RLD domain containing E3 ubiquitin ligase 4 (Herc4), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa17135	NM_138254.3 Latest version!	Drosophila melanogaster HECT and RLD domain containing E3 ubiquitin ligase 4 (Herc4), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa20406	NM_001274320.1 Latest version!	Drosophila melanogaster HECT and RLD domain containing E3 ubiquitin ligase 4 (Herc4), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Herc4 ( NM_138254.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_612098.2 Drosophila melanogaster HECT and RLD domain containing E3 ubiquitin ligase 4 (Herc4), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa17135
Clone ID Related Accession (Same CDS sequence)	NM_138254.3 , NM_167868.2
Accession Version	NM_138254.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	HECT and RLD domain containing E3 ubiquitin ligase 4, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_138254.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATCTG GCAAAGAGCT CTATTGCTGG GGAAGCACGT CACATGGTCA ATTGGGACTT 
GGCGGAATCG AGGACGAGCA GATCCTCACA CCTAGTCAGA TACCCTGGAC ACCGGACACT 
GCTGTACAGC AGGTCGCCTG TGGACACCGA CACACTCTCT TTCTGACCGC CACTGGAAAA 
GTCTACGCTT GCGGGAGCAA CGACTACTCC CAGCTGGGCC ACGATCTGCC CACCAAAAGG 
CCACTGCTTA TCCCCGAACT GCAGGATTAC GTGATCATCC AGATCTGCTG CGGCAGCCGC 
CACTCATTGG CGCTCTCCGA TTGGGGCCAG GTCTTAAGCT GGGGCGACAA CGACTGCGGC 
CAGCTGGGCC ATGCCACCGA TAAGGAGATC GTGCAGCTGC CCAAGGTTGT GCGGCAACTG 
GTCACAAAGA CGGTGGTGCA GATCGCCTGC GGCAACAATC ACAGCTTGGC ACTGACCAGT 
TGTGGCGAGC TGTATTCATG GGGTTCCAAC ATCTACGGTC AGCTGGGCGT GAACTCCCCC 
AATGATTTGA CACATTGCAA CTATCCCCTC CGACTAACCA CTCTGCTGGG AATTCCGCTG 
GCCGCAATTG CCTGTGGAGG CAATCACTCG TTCCTCATCT CCAAGTCCGG TGCGGTGTTC 
GGATGGGGCA GAAACAACTG CGGGCAACTG GGACTGAATG ACGAAACGAA CCGCTCGTAT 
CCCACCCAGC TGAAAACGCT GCGCACCCTG GGCGTGCGAT TTGTGGCCTG CGGCGATGAG 
TTCTCCGTTT TTCTGACCAA CGAGGGAGGG GTGTTCACCT GTGGAGCTGG CGCCTATGGG 
CAGTTGGGTC ATGGCTTTAG CTCGAATGAG ATGCTACCGC GAATGGTAAT GGAGCTAATG 
GGCAGCACCA TCACTCAGGT GGCCTGTGGC AATCGGCACA CTCTGGCGCT TGTTCCATCG 
CGCGGCAGAG TTTATGCCTT TGGCTTGGGT AGCTCTGGGC AGCTGGGCAC GAGGAGTACC 
AAGAGCCTGA TGCTGCCGCA GGTGGTCATC GGTCCTTGGG TGTCGCCCAG CGGATCAGCC 
CTGTTGCAAT CCAATGATTC ACAGGTCTCG TTGGTCATCC GACAGATTTT CTCAGGTGGC 
GATCAGTCGA TTGTGACCAC AACTTTGTTT GTAGACAAGG TGCCGCCCGA GGATTTCAGA 
AACTACAACC CCAAATCCCA GATCCTAACC CTAACAGCAG AAGTCACCAA GCAGTGTGCT 
CAATGCAAGC AGGGCCTTCA AAGCGACATG GATCTACTGA GCTCTATTGA ACTAATATTT 
AGAAGCCAGG CGTGTTGGAA TGGTTCCTTT CTGCTGGATC ACGACCGACA CTTTGGATGC 
TCGGTTCGCA ACCATGGACT GGATCTGAAA GCAGCCCAAC TAGCATTCGA CAATCTGCGC 
GCTGTGGAAA ATGAAAGCAT TAGGCAGGTG ATCTGGGACA ATATAACCAA GGAGCTAGTT 
GGTTCGCTGG TTTGCTCACC GGCAGATGTG GAGAGCATGC GATTATATCT TCTGCTCCCA 
CTTTACCACG AGTTCGTCAA CTCCAAGCAC TACAAGTCAC TGCAGGTTCC GTTTGCCAAT 
GCCATATTTA AGCTAGCAGA GAACCCACGG AAAGTGCTGA ACAAGTGGTT GGCCCAAACA 
CCGGCTGAAT ACTTCGAGCA TCTTGTCCAG AACTTCTTGC ATGTTGTCAT CCATATTATA 
AGTTTCAAAA TGGGCTTGGC TGCCGCTAGT CCCAGCGCCG AAAGACGTCA ACAGCTGCTG 
CCATATAACA CTGAACTCGA GATCATTCTA AAGCTGATGA AAACGCTCTG CCAAATTAAC 
AACGAGCGCC ATGATCGCCT AAACTATCAA ATCTTTTACT GGCCTGATCT TTCGGATTAT 
GCAGACGTGC AGCAGGAGTA CGTTAAATGG ATCATGGCCG ACACTGCCAG AGATTTCAAT 
ATTTGCAACT ATTCATTCAT TTTCGACCAG TCTGCTAAAA CAGCGTTACT CCAAGCGGAT 
CAGGCGCTCC AGATGCACTC CGCCATGGCC AATGCGGCTA CCATGCAGGC ATTCTCCTTT 
TTCAACTACG GGATGCCCAT CTCGCAGTTC ATTGTGCTCA ACGTGACGCG TGAGAATCTG 
GTGCAGGATT CCCTTCGAGA GTTGCAGCAT TATTCGCAGA GTGATCTTAA GAAGCCGCTG 
AAAATCAAGT TCCATGGCGA GGAGGCGGAG GACGCGGGTG GCGTGCGCAA GGAGTTCTTC 
ATGTTGCTGC TCAAGGATCT GCTCGATCCC AAGTACGGTA TGTTTAAGGA GTACGAGCAG 
TCGCGTCTGC TCTGGTTTGC AGATCTCACC TTCGAGACGG AAAACATGTA CTTCCTAATT 
GGCGTTCTCT GCGGTCTGGC CATCTATAAC TTCACCATCA TCAACTTGCC ATTCCCACTG 
GCTCTGTTCA AAAAGCTGCT GGGCAAGCCA GTGGATCTCA GTGATCTCCG TCAGCTTTCC 
CCTCCGGAGG CCAACTCCAT GCAATCGCTG CTCGACTACC AGGGCGACGA TTTTAAGGAA 
GTCTTCGATC TGACCTTTGA GATATCGCGG GATGTATTCG GGGAGGCGGA GACGAAGTGC 
CTTAAGCCCA ACGGCAATGA GATAGCCGTA ACACTGGAAA ACAGGCAGGA GTTTGTGGAT 
CTCTATGTTG ACTTCGTATT CAACAAGTCC GTCGAGCTGC ACTACAATGC GTTTCACAAG 
GGCTTTATGA AGGTGTGCTC TGGCCGCGTG ATTCACATAT TCCAGCCGGA GGAGCTGATG 
GCCGTGGTGG TGGGAAATGA GGATTACGAC TGGCAGGCGC TTCAAGACAA CTGCGAGTAC 
CGGGAGGGCT ACACTTCGGT CGATGATACA ATCAAATGGT TCTGGGAGGT TATTCATGAC 
ATGTCCGAGG CGGAAAAGAA GAGTTTTCTC CTCTTTTTAA CTGGCAGCGA TCGCATTCCC 
ATCCAGGGCA TGAAAGCCCT TAAACTCACC ATTCAACCCA CCCCGGACGA ACGATTCCTG 
CCCGTGGCGC ACACGTGCTT CAATCTGCTG GATCTGCCGC GCTACAAGAC CAAGGAGCGC 
CTCAAGTACA AGCTGCTACA GGCCATCCAG CAGACGCAGG GCTTCAGCCT GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_612098.2
CDS	249..3425
Translation	MASGKELYCWGSTSHGQLGLGGIEDEQILTPSQIPWTPDTAVQQVACGHRHTLFLTATGKVYACGSNDYSQLGHDLPTKRPLLIPELQDYVIIQICCGSRHSLALSDWGQVLSWGDNDCGQLGHATDKEIVQLPKVVRQLVTKTVVQIACGNNHSLALTSCGELYSWGSNIYGQLGVNSPNDLTHCNYPLRLTTLLGIPLAAIACGGNHSFLISKSGAVFGWGRNNCGQLGLNDETNRSYPTQLKTLRTLGVRFVACGDEFSVFLTNEGGVFTCGAGAYGQLGHGFSSNEMLPRMVMELMGSTITQVACGNRHTLALVPSRGRVYAFGLGSSGQLGTRSTKSLMLPQVVIGPWVSPSGSALLQSNDSQVSLVIRQIFSGGDQSIVTTTLFVDKVPPEDFRNYNPKSQILTLTAEVTKQCAQCKQGLQSDMDLLSSIELIFRSQACWNGSFLLDHDRHFGCSVRNHGLDLKAAQLAFDNLRAVENESIRQVIWDNITKELVGSLVCSPADVESMRLYLLLPLYHEFVNSKHYKSLQVPFANAIFKLAENPRKVLNKWLAQTPAEYFEHLVQNFLHVVIHIISFKMGLAAASPSAERRQQLLPYNTELEIILKLMKTLCQINNERHDRLNYQIFYWPDLSDYADVQQEYVKWIMADTARDFNICNYSFIFDQSAKTALLQADQALQMHSAMANAATMQAFSFFNYGMPISQFIVLNVTRENLVQDSLRELQHYSQSDLKKPLKIKFHGEEAEDAGGVRKEFFMLLLKDLLDPKYGMFKEYEQSRLLWFADLTFETENMYFLIGVLCGLAIYNFTIINLPFPLALFKKLLGKPVDLSDLRQLSPPEANSMQSLLDYQGDDFKEVFDLTFEISRDVFGEAETKCLKPNGNEIAVTLENRQEFVDLYVDFVFNKSVELHYNAFHKGFMKVCSGRVIHIFQPEELMAVVVGNEDYDWQALQDNCEYREGYTSVDDTIKWFWEVIHDMSEAEKKSFLLFLTGSDRIPIQGMKALKLTIQPTPDERFLPVAHTCFNLLDLPRYKTKERLKYKLLQAIQQTQGFSLV

Target ORF information:

RefSeq Version	NM_138254.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster HECT and RLD domain containing E3 ubiquitin ligase 4 (Herc4), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138254.3

ORF Insert Sequence:

ATGGCATCTG GCAAAGAGCT CTATTGCTGG GGAAGCACGT CACATGGTCA ATTGGGACTT 
GGCGGAATCG AGGACGAGCA GATCCTCACA CCTAGTCAGA TACCCTGGAC ACCGGACACT 
GCTGTACAGC AGGTCGCCTG TGGACACCGA CACACTCTCT TTCTGACCGC CACTGGAAAA 
GTCTACGCTT GCGGGAGCAA CGACTACTCC CAGCTGGGCC ACGATCTGCC CACCAAAAGG 
CCACTGCTTA TCCCCGAACT GCAGGATTAC GTGATCATCC AGATCTGCTG CGGCAGCCGC 
CACTCATTGG CGCTCTCCGA TTGGGGCCAG GTCTTAAGCT GGGGCGACAA CGACTGCGGC 
CAGCTGGGCC ATGCCACCGA TAAGGAGATC GTGCAGCTGC CCAAGGTTGT GCGGCAACTG 
GTCACAAAGA CGGTGGTGCA GATCGCCTGC GGCAACAATC ACAGCTTGGC ACTGACCAGT 
TGTGGCGAGC TGTATTCATG GGGTTCCAAC ATCTACGGTC AGCTGGGCGT GAACTCCCCC 
AATGATTTGA CACATTGCAA CTATCCCCTC CGACTAACCA CTCTGCTGGG AATTCCGCTG 
GCCGCAATTG CCTGTGGAGG CAATCACTCG TTCCTCATCT CCAAGTCCGG TGCGGTGTTC 
GGATGGGGCA GAAACAACTG CGGGCAACTG GGACTGAATG ACGAAACGAA CCGCTCGTAT 
CCCACCCAGC TGAAAACGCT GCGCACCCTG GGCGTGCGAT TTGTGGCCTG CGGCGATGAG 
TTCTCCGTTT TTCTGACCAA CGAGGGAGGG GTGTTCACCT GTGGAGCTGG CGCCTATGGG 
CAGTTGGGTC ATGGCTTTAG CTCGAATGAG ATGCTACCGC GAATGGTAAT GGAGCTAATG 
GGCAGCACCA TCACTCAGGT GGCCTGTGGC AATCGGCACA CTCTGGCGCT TGTTCCATCG 
CGCGGCAGAG TTTATGCCTT TGGCTTGGGT AGCTCTGGGC AGCTGGGCAC GAGGAGTACC 
AAGAGCCTGA TGCTGCCGCA GGTGGTCATC GGTCCTTGGG TGTCGCCCAG CGGATCAGCC 
CTGTTGCAAT CCAATGATTC ACAGGTCTCG TTGGTCATCC GACAGATTTT CTCAGGTGGC 
GATCAGTCGA TTGTGACCAC AACTTTGTTT GTAGACAAGG TGCCGCCCGA GGATTTCAGA 
AACTACAACC CCAAATCCCA GATCCTAACC CTAACAGCAG AAGTCACCAA GCAGTGTGCT 
CAATGCAAGC AGGGCCTTCA AAGCGACATG GATCTACTGA GCTCTATTGA ACTAATATTT 
AGAAGCCAGG CGTGTTGGAA TGGTTCCTTT CTGCTGGATC ACGACCGACA CTTTGGATGC 
TCGGTTCGCA ACCATGGACT GGATCTGAAA GCAGCCCAAC TAGCATTCGA CAATCTGCGC 
GCTGTGGAAA ATGAAAGCAT TAGGCAGGTG ATCTGGGACA ATATAACCAA GGAGCTAGTT 
GGTTCGCTGG TTTGCTCACC GGCAGATGTG GAGAGCATGC GATTATATCT TCTGCTCCCA 
CTTTACCACG AGTTCGTCAA CTCCAAGCAC TACAAGTCAC TGCAGGTTCC GTTTGCCAAT 
GCCATATTTA AGCTAGCAGA GAACCCACGG AAAGTGCTGA ACAAGTGGTT GGCCCAAACA 
CCGGCTGAAT ACTTCGAGCA TCTTGTCCAG AACTTCTTGC ATGTTGTCAT CCATATTATA 
AGTTTCAAAA TGGGCTTGGC TGCCGCTAGT CCCAGCGCCG AAAGACGTCA ACAGCTGCTG 
CCATATAACA CTGAACTCGA GATCATTCTA AAGCTGATGA AAACGCTCTG CCAAATTAAC 
AACGAGCGCC ATGATCGCCT AAACTATCAA ATCTTTTACT GGCCTGATCT TTCGGATTAT 
GCAGACGTGC AGCAGGAGTA CGTTAAATGG ATCATGGCCG ACACTGCCAG AGATTTCAAT 
ATTTGCAACT ATTCATTCAT TTTCGACCAG TCTGCTAAAA CAGCGTTACT CCAAGCGGAT 
CAGGCGCTCC AGATGCACTC CGCCATGGCC AATGCGGCTA CCATGCAGGC ATTCTCCTTT 
TTCAACTACG GGATGCCCAT CTCGCAGTTC ATTGTGCTCA ACGTGACGCG TGAGAATCTG 
GTGCAGGATT CCCTTCGAGA GTTGCAGCAT TATTCGCAGA GTGATCTTAA GAAGCCGCTG 
AAAATCAAGT TCCATGGCGA GGAGGCGGAG GACGCGGGTG GCGTGCGCAA GGAGTTCTTC 
ATGTTGCTGC TCAAGGATCT GCTCGATCCC AAGTACGGTA TGTTTAAGGA GTACGAGCAG 
TCGCGTCTGC TCTGGTTTGC AGATCTCACC TTCGAGACGG AAAACATGTA CTTCCTAATT 
GGCGTTCTCT GCGGTCTGGC CATCTATAAC TTCACCATCA TCAACTTGCC ATTCCCACTG 
GCTCTGTTCA AAAAGCTGCT GGGCAAGCCA GTGGATCTCA GTGATCTCCG TCAGCTTTCC 
CCTCCGGAGG CCAACTCCAT GCAATCGCTG CTCGACTACC AGGGCGACGA TTTTAAGGAA 
GTCTTCGATC TGACCTTTGA GATATCGCGG GATGTATTCG GGGAGGCGGA GACGAAGTGC 
CTTAAGCCCA ACGGCAATGA GATAGCCGTA ACACTGGAAA ACAGGCAGGA GTTTGTGGAT 
CTCTATGTTG ACTTCGTATT CAACAAGTCC GTCGAGCTGC ACTACAATGC GTTTCACAAG 
GGCTTTATGA AGGTGTGCTC TGGCCGCGTG ATTCACATAT TCCAGCCGGA GGAGCTGATG 
GCCGTGGTGG TGGGAAATGA GGATTACGAC TGGCAGGCGC TTCAAGACAA CTGCGAGTAC 
CGGGAGGGCT ACACTTCGGT CGATGATACA ATCAAATGGT TCTGGGAGGT TATTCATGAC 
ATGTCCGAGG CGGAAAAGAA GAGTTTTCTC CTCTTTTTAA CTGGCAGCGA TCGCATTCCC 
ATCCAGGGCA TGAAAGCCCT TAAACTCACC ATTCAACCCA CCCCGGACGA ACGATTCCTG 
CCCGTGGCGC ACACGTGCTT CAATCTGCTG GATCTGCCGC GCTACAAGAC CAAGGAGCGC 
CTCAAGTACA AGCTGCTACA GGCCATCCAG CAGACGCAGG GCTTCAGCCT GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Herc4 ( NM_167868.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728591.1 Drosophila melanogaster HECT and RLD domain containing E3 ubiquitin ligase 4 (Herc4), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa17135
Clone ID Related Accession (Same CDS sequence)	NM_138254.3 , NM_167868.2
Accession Version	NM_167868.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3177bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	HECT and RLD domain containing E3 ubiquitin ligase 4, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_167868.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATCTG GCAAAGAGCT CTATTGCTGG GGAAGCACGT CACATGGTCA ATTGGGACTT 
GGCGGAATCG AGGACGAGCA GATCCTCACA CCTAGTCAGA TACCCTGGAC ACCGGACACT 
GCTGTACAGC AGGTCGCCTG TGGACACCGA CACACTCTCT TTCTGACCGC CACTGGAAAA 
GTCTACGCTT GCGGGAGCAA CGACTACTCC CAGCTGGGCC ACGATCTGCC CACCAAAAGG 
CCACTGCTTA TCCCCGAACT GCAGGATTAC GTGATCATCC AGATCTGCTG CGGCAGCCGC 
CACTCATTGG CGCTCTCCGA TTGGGGCCAG GTCTTAAGCT GGGGCGACAA CGACTGCGGC 
CAGCTGGGCC ATGCCACCGA TAAGGAGATC GTGCAGCTGC CCAAGGTTGT GCGGCAACTG 
GTCACAAAGA CGGTGGTGCA GATCGCCTGC GGCAACAATC ACAGCTTGGC ACTGACCAGT 
TGTGGCGAGC TGTATTCATG GGGTTCCAAC ATCTACGGTC AGCTGGGCGT GAACTCCCCC 
AATGATTTGA CACATTGCAA CTATCCCCTC CGACTAACCA CTCTGCTGGG AATTCCGCTG 
GCCGCAATTG CCTGTGGAGG CAATCACTCG TTCCTCATCT CCAAGTCCGG TGCGGTGTTC 
GGATGGGGCA GAAACAACTG CGGGCAACTG GGACTGAATG ACGAAACGAA CCGCTCGTAT 
CCCACCCAGC TGAAAACGCT GCGCACCCTG GGCGTGCGAT TTGTGGCCTG CGGCGATGAG 
TTCTCCGTTT TTCTGACCAA CGAGGGAGGG GTGTTCACCT GTGGAGCTGG CGCCTATGGG 
CAGTTGGGTC ATGGCTTTAG CTCGAATGAG ATGCTACCGC GAATGGTAAT GGAGCTAATG 
GGCAGCACCA TCACTCAGGT GGCCTGTGGC AATCGGCACA CTCTGGCGCT TGTTCCATCG 
CGCGGCAGAG TTTATGCCTT TGGCTTGGGT AGCTCTGGGC AGCTGGGCAC GAGGAGTACC 
AAGAGCCTGA TGCTGCCGCA GGTGGTCATC GGTCCTTGGG TGTCGCCCAG CGGATCAGCC 
CTGTTGCAAT CCAATGATTC ACAGGTCTCG TTGGTCATCC GACAGATTTT CTCAGGTGGC 
GATCAGTCGA TTGTGACCAC AACTTTGTTT GTAGACAAGG TGCCGCCCGA GGATTTCAGA 
AACTACAACC CCAAATCCCA GATCCTAACC CTAACAGCAG AAGTCACCAA GCAGTGTGCT 
CAATGCAAGC AGGGCCTTCA AAGCGACATG GATCTACTGA GCTCTATTGA ACTAATATTT 
AGAAGCCAGG CGTGTTGGAA TGGTTCCTTT CTGCTGGATC ACGACCGACA CTTTGGATGC 
TCGGTTCGCA ACCATGGACT GGATCTGAAA GCAGCCCAAC TAGCATTCGA CAATCTGCGC 
GCTGTGGAAA ATGAAAGCAT TAGGCAGGTG ATCTGGGACA ATATAACCAA GGAGCTAGTT 
GGTTCGCTGG TTTGCTCACC GGCAGATGTG GAGAGCATGC GATTATATCT TCTGCTCCCA 
CTTTACCACG AGTTCGTCAA CTCCAAGCAC TACAAGTCAC TGCAGGTTCC GTTTGCCAAT 
GCCATATTTA AGCTAGCAGA GAACCCACGG AAAGTGCTGA ACAAGTGGTT GGCCCAAACA 
CCGGCTGAAT ACTTCGAGCA TCTTGTCCAG AACTTCTTGC ATGTTGTCAT CCATATTATA 
AGTTTCAAAA TGGGCTTGGC TGCCGCTAGT CCCAGCGCCG AAAGACGTCA ACAGCTGCTG 
CCATATAACA CTGAACTCGA GATCATTCTA AAGCTGATGA AAACGCTCTG CCAAATTAAC 
AACGAGCGCC ATGATCGCCT AAACTATCAA ATCTTTTACT GGCCTGATCT TTCGGATTAT 
GCAGACGTGC AGCAGGAGTA CGTTAAATGG ATCATGGCCG ACACTGCCAG AGATTTCAAT 
ATTTGCAACT ATTCATTCAT TTTCGACCAG TCTGCTAAAA CAGCGTTACT CCAAGCGGAT 
CAGGCGCTCC AGATGCACTC CGCCATGGCC AATGCGGCTA CCATGCAGGC ATTCTCCTTT 
TTCAACTACG GGATGCCCAT CTCGCAGTTC ATTGTGCTCA ACGTGACGCG TGAGAATCTG 
GTGCAGGATT CCCTTCGAGA GTTGCAGCAT TATTCGCAGA GTGATCTTAA GAAGCCGCTG 
AAAATCAAGT TCCATGGCGA GGAGGCGGAG GACGCGGGTG GCGTGCGCAA GGAGTTCTTC 
ATGTTGCTGC TCAAGGATCT GCTCGATCCC AAGTACGGTA TGTTTAAGGA GTACGAGCAG 
TCGCGTCTGC TCTGGTTTGC AGATCTCACC TTCGAGACGG AAAACATGTA CTTCCTAATT 
GGCGTTCTCT GCGGTCTGGC CATCTATAAC TTCACCATCA TCAACTTGCC ATTCCCACTG 
GCTCTGTTCA AAAAGCTGCT GGGCAAGCCA GTGGATCTCA GTGATCTCCG TCAGCTTTCC 
CCTCCGGAGG CCAACTCCAT GCAATCGCTG CTCGACTACC AGGGCGACGA TTTTAAGGAA 
GTCTTCGATC TGACCTTTGA GATATCGCGG GATGTATTCG GGGAGGCGGA GACGAAGTGC 
CTTAAGCCCA ACGGCAATGA GATAGCCGTA ACACTGGAAA ACAGGCAGGA GTTTGTGGAT 
CTCTATGTTG ACTTCGTATT CAACAAGTCC GTCGAGCTGC ACTACAATGC GTTTCACAAG 
GGCTTTATGA AGGTGTGCTC TGGCCGCGTG ATTCACATAT TCCAGCCGGA GGAGCTGATG 
GCCGTGGTGG TGGGAAATGA GGATTACGAC TGGCAGGCGC TTCAAGACAA CTGCGAGTAC 
CGGGAGGGCT ACACTTCGGT CGATGATACA ATCAAATGGT TCTGGGAGGT TATTCATGAC 
ATGTCCGAGG CGGAAAAGAA GAGTTTTCTC CTCTTTTTAA CTGGCAGCGA TCGCATTCCC 
ATCCAGGGCA TGAAAGCCCT TAAACTCACC ATTCAACCCA CCCCGGACGA ACGATTCCTG 
CCCGTGGCGC ACACGTGCTT CAATCTGCTG GATCTGCCGC GCTACAAGAC CAAGGAGCGC 
CTCAAGTACA AGCTGCTACA GGCCATCCAG CAGACGCAGG GCTTCAGCCT GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728591.1
CDS	315..3491
Translation	MASGKELYCWGSTSHGQLGLGGIEDEQILTPSQIPWTPDTAVQQVACGHRHTLFLTATGKVYACGSNDYSQLGHDLPTKRPLLIPELQDYVIIQICCGSRHSLALSDWGQVLSWGDNDCGQLGHATDKEIVQLPKVVRQLVTKTVVQIACGNNHSLALTSCGELYSWGSNIYGQLGVNSPNDLTHCNYPLRLTTLLGIPLAAIACGGNHSFLISKSGAVFGWGRNNCGQLGLNDETNRSYPTQLKTLRTLGVRFVACGDEFSVFLTNEGGVFTCGAGAYGQLGHGFSSNEMLPRMVMELMGSTITQVACGNRHTLALVPSRGRVYAFGLGSSGQLGTRSTKSLMLPQVVIGPWVSPSGSALLQSNDSQVSLVIRQIFSGGDQSIVTTTLFVDKVPPEDFRNYNPKSQILTLTAEVTKQCAQCKQGLQSDMDLLSSIELIFRSQACWNGSFLLDHDRHFGCSVRNHGLDLKAAQLAFDNLRAVENESIRQVIWDNITKELVGSLVCSPADVESMRLYLLLPLYHEFVNSKHYKSLQVPFANAIFKLAENPRKVLNKWLAQTPAEYFEHLVQNFLHVVIHIISFKMGLAAASPSAERRQQLLPYNTELEIILKLMKTLCQINNERHDRLNYQIFYWPDLSDYADVQQEYVKWIMADTARDFNICNYSFIFDQSAKTALLQADQALQMHSAMANAATMQAFSFFNYGMPISQFIVLNVTRENLVQDSLRELQHYSQSDLKKPLKIKFHGEEAEDAGGVRKEFFMLLLKDLLDPKYGMFKEYEQSRLLWFADLTFETENMYFLIGVLCGLAIYNFTIINLPFPLALFKKLLGKPVDLSDLRQLSPPEANSMQSLLDYQGDDFKEVFDLTFEISRDVFGEAETKCLKPNGNEIAVTLENRQEFVDLYVDFVFNKSVELHYNAFHKGFMKVCSGRVIHIFQPEELMAVVVGNEDYDWQALQDNCEYREGYTSVDDTIKWFWEVIHDMSEAEKKSFLLFLTGSDRIPIQGMKALKLTIQPTPDERFLPVAHTCFNLLDLPRYKTKERLKYKLLQAIQQTQGFSLV

Target ORF information:

RefSeq Version	NM_167868.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster HECT and RLD domain containing E3 ubiquitin ligase 4 (Herc4), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167868.2

ORF Insert Sequence:

ATGGCATCTG GCAAAGAGCT CTATTGCTGG GGAAGCACGT CACATGGTCA ATTGGGACTT 
GGCGGAATCG AGGACGAGCA GATCCTCACA CCTAGTCAGA TACCCTGGAC ACCGGACACT 
GCTGTACAGC AGGTCGCCTG TGGACACCGA CACACTCTCT TTCTGACCGC CACTGGAAAA 
GTCTACGCTT GCGGGAGCAA CGACTACTCC CAGCTGGGCC ACGATCTGCC CACCAAAAGG 
CCACTGCTTA TCCCCGAACT GCAGGATTAC GTGATCATCC AGATCTGCTG CGGCAGCCGC 
CACTCATTGG CGCTCTCCGA TTGGGGCCAG GTCTTAAGCT GGGGCGACAA CGACTGCGGC 
CAGCTGGGCC ATGCCACCGA TAAGGAGATC GTGCAGCTGC CCAAGGTTGT GCGGCAACTG 
GTCACAAAGA CGGTGGTGCA GATCGCCTGC GGCAACAATC ACAGCTTGGC ACTGACCAGT 
TGTGGCGAGC TGTATTCATG GGGTTCCAAC ATCTACGGTC AGCTGGGCGT GAACTCCCCC 
AATGATTTGA CACATTGCAA CTATCCCCTC CGACTAACCA CTCTGCTGGG AATTCCGCTG 
GCCGCAATTG CCTGTGGAGG CAATCACTCG TTCCTCATCT CCAAGTCCGG TGCGGTGTTC 
GGATGGGGCA GAAACAACTG CGGGCAACTG GGACTGAATG ACGAAACGAA CCGCTCGTAT 
CCCACCCAGC TGAAAACGCT GCGCACCCTG GGCGTGCGAT TTGTGGCCTG CGGCGATGAG 
TTCTCCGTTT TTCTGACCAA CGAGGGAGGG GTGTTCACCT GTGGAGCTGG CGCCTATGGG 
CAGTTGGGTC ATGGCTTTAG CTCGAATGAG ATGCTACCGC GAATGGTAAT GGAGCTAATG 
GGCAGCACCA TCACTCAGGT GGCCTGTGGC AATCGGCACA CTCTGGCGCT TGTTCCATCG 
CGCGGCAGAG TTTATGCCTT TGGCTTGGGT AGCTCTGGGC AGCTGGGCAC GAGGAGTACC 
AAGAGCCTGA TGCTGCCGCA GGTGGTCATC GGTCCTTGGG TGTCGCCCAG CGGATCAGCC 
CTGTTGCAAT CCAATGATTC ACAGGTCTCG TTGGTCATCC GACAGATTTT CTCAGGTGGC 
GATCAGTCGA TTGTGACCAC AACTTTGTTT GTAGACAAGG TGCCGCCCGA GGATTTCAGA 
AACTACAACC CCAAATCCCA GATCCTAACC CTAACAGCAG AAGTCACCAA GCAGTGTGCT 
CAATGCAAGC AGGGCCTTCA AAGCGACATG GATCTACTGA GCTCTATTGA ACTAATATTT 
AGAAGCCAGG CGTGTTGGAA TGGTTCCTTT CTGCTGGATC ACGACCGACA CTTTGGATGC 
TCGGTTCGCA ACCATGGACT GGATCTGAAA GCAGCCCAAC TAGCATTCGA CAATCTGCGC 
GCTGTGGAAA ATGAAAGCAT TAGGCAGGTG ATCTGGGACA ATATAACCAA GGAGCTAGTT 
GGTTCGCTGG TTTGCTCACC GGCAGATGTG GAGAGCATGC GATTATATCT TCTGCTCCCA 
CTTTACCACG AGTTCGTCAA CTCCAAGCAC TACAAGTCAC TGCAGGTTCC GTTTGCCAAT 
GCCATATTTA AGCTAGCAGA GAACCCACGG AAAGTGCTGA ACAAGTGGTT GGCCCAAACA 
CCGGCTGAAT ACTTCGAGCA TCTTGTCCAG AACTTCTTGC ATGTTGTCAT CCATATTATA 
AGTTTCAAAA TGGGCTTGGC TGCCGCTAGT CCCAGCGCCG AAAGACGTCA ACAGCTGCTG 
CCATATAACA CTGAACTCGA GATCATTCTA AAGCTGATGA AAACGCTCTG CCAAATTAAC 
AACGAGCGCC ATGATCGCCT AAACTATCAA ATCTTTTACT GGCCTGATCT TTCGGATTAT 
GCAGACGTGC AGCAGGAGTA CGTTAAATGG ATCATGGCCG ACACTGCCAG AGATTTCAAT 
ATTTGCAACT ATTCATTCAT TTTCGACCAG TCTGCTAAAA CAGCGTTACT CCAAGCGGAT 
CAGGCGCTCC AGATGCACTC CGCCATGGCC AATGCGGCTA CCATGCAGGC ATTCTCCTTT 
TTCAACTACG GGATGCCCAT CTCGCAGTTC ATTGTGCTCA ACGTGACGCG TGAGAATCTG 
GTGCAGGATT CCCTTCGAGA GTTGCAGCAT TATTCGCAGA GTGATCTTAA GAAGCCGCTG 
AAAATCAAGT TCCATGGCGA GGAGGCGGAG GACGCGGGTG GCGTGCGCAA GGAGTTCTTC 
ATGTTGCTGC TCAAGGATCT GCTCGATCCC AAGTACGGTA TGTTTAAGGA GTACGAGCAG 
TCGCGTCTGC TCTGGTTTGC AGATCTCACC TTCGAGACGG AAAACATGTA CTTCCTAATT 
GGCGTTCTCT GCGGTCTGGC CATCTATAAC TTCACCATCA TCAACTTGCC ATTCCCACTG 
GCTCTGTTCA AAAAGCTGCT GGGCAAGCCA GTGGATCTCA GTGATCTCCG TCAGCTTTCC 
CCTCCGGAGG CCAACTCCAT GCAATCGCTG CTCGACTACC AGGGCGACGA TTTTAAGGAA 
GTCTTCGATC TGACCTTTGA GATATCGCGG GATGTATTCG GGGAGGCGGA GACGAAGTGC 
CTTAAGCCCA ACGGCAATGA GATAGCCGTA ACACTGGAAA ACAGGCAGGA GTTTGTGGAT 
CTCTATGTTG ACTTCGTATT CAACAAGTCC GTCGAGCTGC ACTACAATGC GTTTCACAAG 
GGCTTTATGA AGGTGTGCTC TGGCCGCGTG ATTCACATAT TCCAGCCGGA GGAGCTGATG 
GCCGTGGTGG TGGGAAATGA GGATTACGAC TGGCAGGCGC TTCAAGACAA CTGCGAGTAC 
CGGGAGGGCT ACACTTCGGT CGATGATACA ATCAAATGGT TCTGGGAGGT TATTCATGAC 
ATGTCCGAGG CGGAAAAGAA GAGTTTTCTC CTCTTTTTAA CTGGCAGCGA TCGCATTCCC 
ATCCAGGGCA TGAAAGCCCT TAAACTCACC ATTCAACCCA CCCCGGACGA ACGATTCCTG 
CCCGTGGCGC ACACGTGCTT CAATCTGCTG GATCTGCCGC GCTACAAGAC CAAGGAGCGC 
CTCAAGTACA AGCTGCTACA GGCCATCCAG CAGACGCAGG GCTTCAGCCT GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Herc4 ( NM_001274320.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261249.1 Drosophila melanogaster HECT and RLD domain containing E3 ubiquitin ligase 4 (Herc4), transcript variant C, mRNA.

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CloneID	OFa20406
Clone ID Related Accession (Same CDS sequence)	NM_001274320.1
Accession Version	NM_001274320.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	HECT and RLD domain containing E3 ubiquitin ligase 4, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATCTG GCAAAGAGCT CTATTGCTGG GGAAGCACGT CACATGGTCA ATTGGGACTT 
GGCGGAATCG AGGACGAGCA GATCCTCACA CCTAGTCAGA TACCCTGGAC ACCGGACACT 
GCTGTACAGC AGGTCGCCTG TGGACACCGA CACACTCTCT TTCTGACCGC CACTGGAAAA 
GTCTACGCTT GCGGGAGCAA CGACTACTCC CAGCTGGGCC ACGATCTGCC CACCAAAAGG 
CCACGTATGT CGCCATTTCT GCTTATCCCC GAACTGCAGG ATTACGTGAT CATCCAGATC 
TGCTGCGGCA GCCGCCACTC ATTGGCGCTC TCCGATTGGG GCCAGGTCTT AAGCTGGGGC 
GACAACGACT GCGGCCAGCT GGGCCATGCC ACCGATAAGG AGATCGTGCA GCTGCCCAAG 
GTTGTGCGGC AACTGGTCAC AAAGACGGTG GTGCAGATCG CCTGCGGCAA CAATCACAGC 
TTGGCACTGA CCAGTTGTGG CGAGCTGTAT TCATGGGGTT CCAACATCTA CGGTCAGCTG 
GGCGTGAACT CCCCCAATGA TTTGACACAT TGCAACTATC CCCTCCGACT AACCACTCTG 
CTGGGAATTC CGCTGGCCGC AATTGCCTGT GGAGGCAATC ACTCGTTCCT CATCTCCAAG 
TCCGGTGCGG TGTTCGGATG GGGCAGAAAC AACTGCGGGC AACTGGGACT GAATGACGAA 
ACGAACCGCT CGTATCCCAC CCAGCTGAAA ACGCTGCGCA CCCTGGGCGT GCGATTTGTG 
GCCTGCGGCG ATGAGTTCTC CGTTTTTCTG ACCAACGAGG GAGGGGTGTT CACCTGTGGA 
GCTGGCGCCT ATGGGCAGTT GGGTCATGGC TTTAGCTCGA ATGAGATGCT ACCGCGAATG 
GTAATGGAGC TAATGGGCAG CACCATCACT CAGGTGGCCT GTGGCAATCG GCACACTCTG 
GCGCTTGTTC CATCGCGCGG CAGAGTTTAT GCCTTTGGCT TGGGTAGCTC TGGGCAGCTG 
GGCACGAGGA GTACCAAGAG CCTGATGCTG CCGCAGGTGG TCATCGGTCC TTGGGTGTCG 
CCCAGCGGAT CAGCCCTGTT GCAATCCAAT GATTCACAGG TCTCGTTGGT CATCCGACAG 
ATTTTCTCAG GTGGCGATCA GTCGATTGTG ACCACAACTT TGTTTGTAGA CAAGGTGCCG 
CCCGAGGATT TCAGAAACTA CAACCCCAAA TCCCAGATCC TAACCCTAAC AGCAGAAGTC 
ACCAAGCAGT GTGCTCAATG CAAGCAGGGC CTTCAAAGCG ACATGGATCT ACTGAGCTCT 
ATTGAACTAA TATTTAGAAG CCAGGCGTGT TGGAATGGTT CCTTTCTGCT GGATCACGAC 
CGACACTTTG GATGCTCGGT TCGCAACCAT GGACTGGATC TGAAAGCAGC CCAACTAGCA 
TTCGACAATC TGCGCGCTGT GGAAAATGAA AGCATTAGGC AGGTGATCTG GGACAATATA 
ACCAAGGAGC TAGTTGGTTC GCTGGTTTGC TCACCGGCAG ATGTGGAGAG CATGCGATTA 
TATCTTCTGC TCCCACTTTA CCACGAGTTC GTCAACTCCA AGCACTACAA GTCACTGCAG 
GTTCCGTTTG CCAATGCCAT ATTTAAGCTA GCAGAGAACC CACGGAAAGT GCTGAACAAG 
TGGTTGGCCC AAACACCGGC TGAATACTTC GAGCATCTTG TCCAGAACTT CTTGCATGTT 
GTCATCCATA TTATAAGTTT CAAAATGGGC TTGGCTGCCG CTAGTCCCAG CGCCGAAAGA 
CGTCAACAGC TGCTGCCATA TAACACTGAA CTCGAGATCA TTCTAAAGCT GATGAAAACG 
CTCTGCCAAA TTAACAACGA GCGCCATGAT CGCCTAAACT ATCAAATCTT TTACTGGCCT 
GATCTTTCGG ATTATGCAGA CGTGCAGCAG GAGTACGTTA AATGGATCAT GGCCGACACT 
GCCAGAGATT TCAATATTTG CAACTATTCA TTCATTTTCG ACCAGTCTGC TAAAACAGCG 
TTACTCCAAG CGGATCAGGC GCTCCAGATG CACTCCGCCA TGGCCAATGC GGCTACCATG 
GCATTCTCCT TTTTCAACTA CGGGATGCCC ATCTCGCAGT TCATTGTGCT CAACGTGACG 
CGTGAGAATC TGGTGCAGGA TTCCCTTCGA GAGTTGCAGC ATTATTCGCA GAGTGATCTT 
AAGAAGCCGC TGAAAATCAA GTTCCATGGC GAGGAGGCGG AGGACGCGGG TGGCGTGCGC 
AAGGAGTTCT TCATGTTGCT GCTCAAGGAT CTGCTCGATC CCAAGTACGG TATGTTTAAG 
GAGTACGAGC AGTCGCGTCT GCTCTGGTTT GCAGATCTCA CCTTCGAGAC GGAAAACATG 
TACTTCCTAA TTGGCGTTCT CTGCGGTCTG GCCATCTATA ACTTCACCAT CATCAACTTG 
CCATTCCCAC TGGCTCTGTT CAAAAAGCTG CTGGGCAAGC CAGTGGATCT CAGTGATCTC 
CGTCAGCTTT CCCCTCCGGA GGCCAACTCC ATGCAATCGC TGCTCGACTA CCAGGGCGAC 
GATTTTAAGG AAGTCTTCGA TCTGACCTTT GAGATATCGC GGGATGTATT CGGGGAGGCG 
GAGACGAAGT GCCTTAAGCC CAACGGCAAT GAGATAGCCG TAACACTGGA AAACAGGCAG 
GAGTTTGTGG ATCTCTATGT TGACTTCGTA TTCAACAAGT CCGTCGAGCT GCACTACAAT 
GCGTTTCACA AGGGCTTTAT GAAGGTGTGC TCTGGCCGCG TGATTCACAT ATTCCAGCCG 
GAGGAGCTGA TGGCCGTGGT GGTGGGAAAT GAGGATTACG ACTGGCAGGC GCTTCAAGAC 
AACTGCGAGT ACCGGGAGGG CTACACTTCG GTCGATGATA CAATCAAATG GTTCTGGGAG 
GTTATTCATG ACATGTCCGA GGCGGAAAAG AAGAGTTTTC TCCTCTTTTT AACTGGCAGC 
GATCGCATTC CCATCCAGGG CATGAAAGCC CTTAAACTCA CCATTCAACC CACCCCGGAC 
GAACGATTCC TGCCCGTGGC GCACACGTGC TTCAATCTGC TGGATCTGCC GCGCTACAAG 
ACCAAGGAGC GCCTCAAGTA CAAGCTGCTA CAGGCCATCC AGCAGACGCA GGGCTTCAGC 
CTGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261249.1
CDS	249..3437
Translation	MASGKELYCWGSTSHGQLGLGGIEDEQILTPSQIPWTPDTAVQQVACGHRHTLFLTATGKVYACGSNDYSQLGHDLPTKRPRMSPFLLIPELQDYVIIQICCGSRHSLALSDWGQVLSWGDNDCGQLGHATDKEIVQLPKVVRQLVTKTVVQIACGNNHSLALTSCGELYSWGSNIYGQLGVNSPNDLTHCNYPLRLTTLLGIPLAAIACGGNHSFLISKSGAVFGWGRNNCGQLGLNDETNRSYPTQLKTLRTLGVRFVACGDEFSVFLTNEGGVFTCGAGAYGQLGHGFSSNEMLPRMVMELMGSTITQVACGNRHTLALVPSRGRVYAFGLGSSGQLGTRSTKSLMLPQVVIGPWVSPSGSALLQSNDSQVSLVIRQIFSGGDQSIVTTTLFVDKVPPEDFRNYNPKSQILTLTAEVTKQCAQCKQGLQSDMDLLSSIELIFRSQACWNGSFLLDHDRHFGCSVRNHGLDLKAAQLAFDNLRAVENESIRQVIWDNITKELVGSLVCSPADVESMRLYLLLPLYHEFVNSKHYKSLQVPFANAIFKLAENPRKVLNKWLAQTPAEYFEHLVQNFLHVVIHIISFKMGLAAASPSAERRQQLLPYNTELEIILKLMKTLCQINNERHDRLNYQIFYWPDLSDYADVQQEYVKWIMADTARDFNICNYSFIFDQSAKTALLQADQALQMHSAMANAATMAFSFFNYGMPISQFIVLNVTRENLVQDSLRELQHYSQSDLKKPLKIKFHGEEAEDAGGVRKEFFMLLLKDLLDPKYGMFKEYEQSRLLWFADLTFETENMYFLIGVLCGLAIYNFTIINLPFPLALFKKLLGKPVDLSDLRQLSPPEANSMQSLLDYQGDDFKEVFDLTFEISRDVFGEAETKCLKPNGNEIAVTLENRQEFVDLYVDFVFNKSVELHYNAFHKGFMKVCSGRVIHIFQPEELMAVVVGNEDYDWQALQDNCEYREGYTSVDDTIKWFWEVIHDMSEAEKKSFLLFLTGSDRIPIQGMKALKLTIQPTPDERFLPVAHTCFNLLDLPRYKTKERLKYKLLQAIQQTQGFSLV

Target ORF information:

RefSeq Version	NM_001274320.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster HECT and RLD domain containing E3 ubiquitin ligase 4 (Herc4), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274320.1

ORF Insert Sequence:

ATGGCATCTG GCAAAGAGCT CTATTGCTGG GGAAGCACGT CACATGGTCA ATTGGGACTT 
GGCGGAATCG AGGACGAGCA GATCCTCACA CCTAGTCAGA TACCCTGGAC ACCGGACACT 
GCTGTACAGC AGGTCGCCTG TGGACACCGA CACACTCTCT TTCTGACCGC CACTGGAAAA 
GTCTACGCTT GCGGGAGCAA CGACTACTCC CAGCTGGGCC ACGATCTGCC CACCAAAAGG 
CCACGTATGT CGCCATTTCT GCTTATCCCC GAACTGCAGG ATTACGTGAT CATCCAGATC 
TGCTGCGGCA GCCGCCACTC ATTGGCGCTC TCCGATTGGG GCCAGGTCTT AAGCTGGGGC 
GACAACGACT GCGGCCAGCT GGGCCATGCC ACCGATAAGG AGATCGTGCA GCTGCCCAAG 
GTTGTGCGGC AACTGGTCAC AAAGACGGTG GTGCAGATCG CCTGCGGCAA CAATCACAGC 
TTGGCACTGA CCAGTTGTGG CGAGCTGTAT TCATGGGGTT CCAACATCTA CGGTCAGCTG 
GGCGTGAACT CCCCCAATGA TTTGACACAT TGCAACTATC CCCTCCGACT AACCACTCTG 
CTGGGAATTC CGCTGGCCGC AATTGCCTGT GGAGGCAATC ACTCGTTCCT CATCTCCAAG 
TCCGGTGCGG TGTTCGGATG GGGCAGAAAC AACTGCGGGC AACTGGGACT GAATGACGAA 
ACGAACCGCT CGTATCCCAC CCAGCTGAAA ACGCTGCGCA CCCTGGGCGT GCGATTTGTG 
GCCTGCGGCG ATGAGTTCTC CGTTTTTCTG ACCAACGAGG GAGGGGTGTT CACCTGTGGA 
GCTGGCGCCT ATGGGCAGTT GGGTCATGGC TTTAGCTCGA ATGAGATGCT ACCGCGAATG 
GTAATGGAGC TAATGGGCAG CACCATCACT CAGGTGGCCT GTGGCAATCG GCACACTCTG 
GCGCTTGTTC CATCGCGCGG CAGAGTTTAT GCCTTTGGCT TGGGTAGCTC TGGGCAGCTG 
GGCACGAGGA GTACCAAGAG CCTGATGCTG CCGCAGGTGG TCATCGGTCC TTGGGTGTCG 
CCCAGCGGAT CAGCCCTGTT GCAATCCAAT GATTCACAGG TCTCGTTGGT CATCCGACAG 
ATTTTCTCAG GTGGCGATCA GTCGATTGTG ACCACAACTT TGTTTGTAGA CAAGGTGCCG 
CCCGAGGATT TCAGAAACTA CAACCCCAAA TCCCAGATCC TAACCCTAAC AGCAGAAGTC 
ACCAAGCAGT GTGCTCAATG CAAGCAGGGC CTTCAAAGCG ACATGGATCT ACTGAGCTCT 
ATTGAACTAA TATTTAGAAG CCAGGCGTGT TGGAATGGTT CCTTTCTGCT GGATCACGAC 
CGACACTTTG GATGCTCGGT TCGCAACCAT GGACTGGATC TGAAAGCAGC CCAACTAGCA 
TTCGACAATC TGCGCGCTGT GGAAAATGAA AGCATTAGGC AGGTGATCTG GGACAATATA 
ACCAAGGAGC TAGTTGGTTC GCTGGTTTGC TCACCGGCAG ATGTGGAGAG CATGCGATTA 
TATCTTCTGC TCCCACTTTA CCACGAGTTC GTCAACTCCA AGCACTACAA GTCACTGCAG 
GTTCCGTTTG CCAATGCCAT ATTTAAGCTA GCAGAGAACC CACGGAAAGT GCTGAACAAG 
TGGTTGGCCC AAACACCGGC TGAATACTTC GAGCATCTTG TCCAGAACTT CTTGCATGTT 
GTCATCCATA TTATAAGTTT CAAAATGGGC TTGGCTGCCG CTAGTCCCAG CGCCGAAAGA 
CGTCAACAGC TGCTGCCATA TAACACTGAA CTCGAGATCA TTCTAAAGCT GATGAAAACG 
CTCTGCCAAA TTAACAACGA GCGCCATGAT CGCCTAAACT ATCAAATCTT TTACTGGCCT 
GATCTTTCGG ATTATGCAGA CGTGCAGCAG GAGTACGTTA AATGGATCAT GGCCGACACT 
GCCAGAGATT TCAATATTTG CAACTATTCA TTCATTTTCG ACCAGTCTGC TAAAACAGCG 
TTACTCCAAG CGGATCAGGC GCTCCAGATG CACTCCGCCA TGGCCAATGC GGCTACCATG 
GCATTCTCCT TTTTCAACTA CGGGATGCCC ATCTCGCAGT TCATTGTGCT CAACGTGACG 
CGTGAGAATC TGGTGCAGGA TTCCCTTCGA GAGTTGCAGC ATTATTCGCA GAGTGATCTT 
AAGAAGCCGC TGAAAATCAA GTTCCATGGC GAGGAGGCGG AGGACGCGGG TGGCGTGCGC 
AAGGAGTTCT TCATGTTGCT GCTCAAGGAT CTGCTCGATC CCAAGTACGG TATGTTTAAG 
GAGTACGAGC AGTCGCGTCT GCTCTGGTTT GCAGATCTCA CCTTCGAGAC GGAAAACATG 
TACTTCCTAA TTGGCGTTCT CTGCGGTCTG GCCATCTATA ACTTCACCAT CATCAACTTG 
CCATTCCCAC TGGCTCTGTT CAAAAAGCTG CTGGGCAAGC CAGTGGATCT CAGTGATCTC 
CGTCAGCTTT CCCCTCCGGA GGCCAACTCC ATGCAATCGC TGCTCGACTA CCAGGGCGAC 
GATTTTAAGG AAGTCTTCGA TCTGACCTTT GAGATATCGC GGGATGTATT CGGGGAGGCG 
GAGACGAAGT GCCTTAAGCC CAACGGCAAT GAGATAGCCG TAACACTGGA AAACAGGCAG 
GAGTTTGTGG ATCTCTATGT TGACTTCGTA TTCAACAAGT CCGTCGAGCT GCACTACAAT 
GCGTTTCACA AGGGCTTTAT GAAGGTGTGC TCTGGCCGCG TGATTCACAT ATTCCAGCCG 
GAGGAGCTGA TGGCCGTGGT GGTGGGAAAT GAGGATTACG ACTGGCAGGC GCTTCAAGAC 
AACTGCGAGT ACCGGGAGGG CTACACTTCG GTCGATGATA CAATCAAATG GTTCTGGGAG 
GTTATTCATG ACATGTCCGA GGCGGAAAAG AAGAGTTTTC TCCTCTTTTT AACTGGCAGC 
GATCGCATTC CCATCCAGGG CATGAAAGCC CTTAAACTCA CCATTCAACC CACCCCGGAC 
GAACGATTCC TGCCCGTGGC GCACACGTGC TTCAATCTGC TGGATCTGCC GCGCTACAAG 
ACCAAGGAGC GCCTCAAGTA CAAGCTGCTA CAGGCCATCC AGCAGACGCA GGGCTTCAGC 
CTGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Herc4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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