Sf3b3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sf3b3
Entrez Gene ID	38093
Full Name	CG13900 gene product from transcript CG13900-RA
Synonyms	Dmel\CG13900,SF3B3
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 61D2-61D2

mRNA and Protein(s)

mRNA	Protein	Name
NM_167842.3	NP_728546.1	splicing factor 3b subunit 3, isoform A
NM_001274294.2	NP_001261223.1	splicing factor 3b subunit 3, isoform C

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SF3B3	XP_536791.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005549	XP_315551.1
Homo sapiens (human)	SF3B3	NP_036558.3
Bos taurus (cattle)	SF3B3	NP_001071319.1
Rattus norvegicus (Norway rat)	Sf3b3	NP_001099657.2
Gallus gallus (chicken)	SF3B3	XP_004944176.1
Danio rerio (zebrafish)	LOC100334114	XP_002667683.2
Magnaporthe oryzae (rice blast fungus)	MGG_06457	XP_003717109.1
Neurospora crassa	NCU00396	XP_957282.2
Xenopus tropicalis (tropical clawed frog)	sf3b3	XP_002931705.1
	SF3B3	XP_002802591.1
Mus musculus (house mouse)	Sf3b3	NP_598714.1
Caenorhabditis elegans (roundworm)	teg-4	NP_491953.1
Arabidopsis thaliana (thale cress)	AT3G55220	NP_567016.1
Pan troglodytes (chimpanzee)	SF3B3	XP_511081.3
Danio rerio (zebrafish)	sf3b3	NP_998668.1
Drosophila melanogaster (fruit fly)	CG13900	NP_728546.1
Schizosaccharomyces pombe (fission yeast)	prp12	NP_594414.1
Arabidopsis thaliana (thale cress)	AT3G55200	NP_567015.1
Oryza sativa (rice)	Os02g0137400	NP_001045829.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Sf3b3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sf3b3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17084	NM_001274294.2 Latest version!	Drosophila melanogaster splicing factor 3b subunit 3, transcript variant C (Sf3b3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa20386	NM_167842.3 Latest version!	Drosophila melanogaster splicing factor 3b subunit 3, transcript variant A (Sf3b3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sf3b3 ( NM_001274294.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261223.1 Drosophila melanogaster splicing factor 3b subunit 3, transcript variant C (Sf3b3), mRNA.

Price & Availability↑

CloneID	OFa17084
Clone ID Related Accession (Same CDS sequence)	NM_001274294.2
Accession Version	NM_001274294.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	splicing factor 3b subunit 3, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_001274294.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTATCTGT ACAACCTGAC CCTGCAGAAG GCCACCGGCG TGACCCACGC GGTCCACGGC 
AACTTCTCCG GCGGCAAGCA GCAGGAGGTC CTCCTGTCGC GTGGCAAGTC GCTGGAGCTA 
CTGCGTCCGG ACTCCAACAC CGGCAAGGTG CACACCCTGC TCTCCACGGA GATATTCGGA 
TGCGTTCGCG CCCTGATGGC CTTTCGACTG ACGGGCGGCA CTAAAGATTA CATAGTTGTG 
GGCTCGGACT CGGGACGCAT CGTGATCCTC GAGTACAATC CCTCCAAGAA TGCTCTTGAA 
AAAGTGCACC AGGAGACGTT CGGAAAGTCG GGATGTCGCA GGATTGTGCC TGGGCAATAC 
TTTGCCATCG ATCCTAAAGG AAGAGCCGTG ATGATTGGCG CCGTGGAGAA GCAAAAGCTG 
GCCTACATCA TGAACCGGGA CACGCAGGCT CGCCTCACGA TCTCATCGCC CCTGGAGGCC 
CACAAATCGA ATACACTGAC ATACCACATG GTCGGCGTGG ATGTGGGCTT CGATAATCCC 
ATGTTCGCCT GCCTAGAGAT CGACTACGAA GAGGCCGACA TGGATCCATC GGGTGACGCC 
GCCCAGCGCA CTCAGCAAAC GCTGACTTTC TACGAGTTGG ATCTCGGCTT AAACCACGTG 
GTTCGCAAAT ACTCGGAGCC CTTGGAAGAG CACGCCAATT TCTTGGTATC CGTGCCTGGG 
GGCAACGATG GTCCTTCGGG CGTGCTGATC TGCTCGGAGA ACTACCTAAC GTACAAGAAT 
CTCGGCGATC AGCACGATAT CCGCTGTCCC ATTCCGCGCA GGAGAAACGA TCTGGATGAT 
CCCGAACGTG GTATGATCTT CATCTGCTCG GCCACACATC GCACCAAGAG CATGTACTTC 
TTCCTGCTGC AGACCGAGCA GGGAGACATC TTTAAGATCA CCCTGGAAAC GGACGATGAT 
GTGGTCTCCG AGATCAAGCT AAAGTACTTC GATACAGTGC CGCCGGCAAC TGCCATGTGC 
GTGCTAAAGA CGGGTTTCCT GTTCGTGGCC AGCGAGTTTG GCAACCACTA TCTATATCAA 
ATCGCTCATC TGGGTGACGA CGATGACGAG CCGGAGTTCA GTTCGGCCAT GCCTTTGGAG 
GAGGGTGAAA CCTTCTTCTT TGCGCCACGT GCCCTAAAGA ACCTGGTGCT TGTGGATGAA 
CTGCCCTCTT TCGCACCGAT CATCACCTCC CAGGTGGCTG ATCTGGCCAA CGAGGACACA 
CCGCAACTGT ACGTTCTTTG CGGACGAGGA CCGCGTTCCA CTCTGCGGGT GCTGCGCCAC 
GGCCTCGAGG TGTCCGAGAT GGCCGTTTCC GAACTGCCTG GTAATCCCAA TGCGGTTTGG 
ACTGTGAAGA AACGAGCTGA TGGCGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261223.1
CDS	162..1571
Translation	MYLYNLTLQKATGVTHAVHGNFSGGKQQEVLLSRGKSLELLRPDSNTGKVHTLLSTEIFGCVRALMAFRLTGGTKDYIVVGSDSGRIVILEYNPSKNALEKVHQETFGKSGCRRIVPGQYFAIDPKGRAVMIGAVEKQKLAYIMNRDTQARLTISSPLEAHKSNTLTYHMVGVDVGFDNPMFACLEIDYEEADMDPSGDAAQRTQQTLTFYELDLGLNHVVRKYSEPLEEHANFLVSVPGGNDGPSGVLICSENYLTYKNLGDQHDIRCPIPRRRNDLDDPERGMIFICSATHRTKSMYFFLLQTEQGDIFKITLETDDDVVSEIKLKYFDTVPPATAMCVLKTGFLFVASEFGNHYLYQIAHLGDDDDEPEFSSAMPLEEGETFFFAPRALKNLVLVDELPSFAPIITSQVADLANEDTPQLYVLCGRGPRSTLRVLRHGLEVSEMAVSELPGNPNAVWTVKKRADGA

Target ORF information:

RefSeq Version	NM_001274294.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster splicing factor 3b subunit 3, transcript variant C (Sf3b3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274294.2

ORF Insert Sequence:

ATGTATCTGT ACAACCTGAC CCTGCAGAAG GCCACCGGCG TGACCCACGC GGTCCACGGC 
AACTTCTCCG GCGGCAAGCA GCAGGAGGTC CTCCTGTCGC GTGGCAAGTC GCTGGAGCTA 
CTGCGTCCGG ACTCCAACAC CGGCAAGGTG CACACCCTGC TCTCCACGGA GATATTCGGA 
TGCGTTCGCG CCCTGATGGC CTTTCGACTG ACGGGCGGCA CTAAAGATTA CATAGTTGTG 
GGCTCGGACT CGGGACGCAT CGTGATCCTC GAGTACAATC CCTCCAAGAA TGCTCTTGAA 
AAAGTGCACC AGGAGACGTT CGGAAAGTCG GGATGTCGCA GGATTGTGCC TGGGCAATAC 
TTTGCCATCG ATCCTAAAGG AAGAGCCGTG ATGATTGGCG CCGTGGAGAA GCAAAAGCTG 
GCCTACATCA TGAACCGGGA CACGCAGGCT CGCCTCACGA TCTCATCGCC CCTGGAGGCC 
CACAAATCGA ATACACTGAC ATACCACATG GTCGGCGTGG ATGTGGGCTT CGATAATCCC 
ATGTTCGCCT GCCTAGAGAT CGACTACGAA GAGGCCGACA TGGATCCATC GGGTGACGCC 
GCCCAGCGCA CTCAGCAAAC GCTGACTTTC TACGAGTTGG ATCTCGGCTT AAACCACGTG 
GTTCGCAAAT ACTCGGAGCC CTTGGAAGAG CACGCCAATT TCTTGGTATC CGTGCCTGGG 
GGCAACGATG GTCCTTCGGG CGTGCTGATC TGCTCGGAGA ACTACCTAAC GTACAAGAAT 
CTCGGCGATC AGCACGATAT CCGCTGTCCC ATTCCGCGCA GGAGAAACGA TCTGGATGAT 
CCCGAACGTG GTATGATCTT CATCTGCTCG GCCACACATC GCACCAAGAG CATGTACTTC 
TTCCTGCTGC AGACCGAGCA GGGAGACATC TTTAAGATCA CCCTGGAAAC GGACGATGAT 
GTGGTCTCCG AGATCAAGCT AAAGTACTTC GATACAGTGC CGCCGGCAAC TGCCATGTGC 
GTGCTAAAGA CGGGTTTCCT GTTCGTGGCC AGCGAGTTTG GCAACCACTA TCTATATCAA 
ATCGCTCATC TGGGTGACGA CGATGACGAG CCGGAGTTCA GTTCGGCCAT GCCTTTGGAG 
GAGGGTGAAA CCTTCTTCTT TGCGCCACGT GCCCTAAAGA ACCTGGTGCT TGTGGATGAA 
CTGCCCTCTT TCGCACCGAT CATCACCTCC CAGGTGGCTG ATCTGGCCAA CGAGGACACA 
CCGCAACTGT ACGTTCTTTG CGGACGAGGA CCGCGTTCCA CTCTGCGGGT GCTGCGCCAC 
GGCCTCGAGG TGTCCGAGAT GGCCGTTTCC GAACTGCCTG GTAATCCCAA TGCGGTTTGG 
ACTGTGAAGA AACGAGCTGA TGGCGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sf3b3 ( NM_167842.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728546.1 Drosophila melanogaster splicing factor 3b subunit 3, transcript variant A (Sf3b3), mRNA.

Price & Availability↑

CloneID	OFa20386
Clone ID Related Accession (Same CDS sequence)	NM_167842.3
Accession Version	NM_167842.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	splicing factor 3b subunit 3, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jul 15, 2014 this sequence version replaced NM_167842.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTATCTGT ACAACCTGAC CCTGCAGAAG GCCACCGGCG TGACCCACGC GGTCCACGGC 
AACTTCTCCG GCGGCAAGCA GCAGGAGGTC CTCCTGTCGC GTGGCAAGTC GCTGGAGCTA 
CTGCGTCCGG ACTCCAACAC CGGCAAGGTG CACACCCTGC TCTCCACGGA GATATTCGGA 
TGCGTTCGCG CCCTGATGGC CTTTCGACTG ACGGGCGGCA CTAAAGATTA CATAGTTGTG 
GGCTCGGACT CGGGACGCAT CGTGATCCTC GAGTACAATC CCTCCAAGAA TGCTCTTGAA 
AAAGTGCACC AGGAGACGTT CGGAAAGTCG GGATGTCGCA GGATTGTGCC TGGGCAATAC 
TTTGCCATCG ATCCTAAAGG AAGAGCCGTG ATGATTGGCG CCGTGGAGAA GCAAAAGCTG 
GCCTACATCA TGAACCGGGA CACGCAGGCT CGCCTCACGA TCTCATCGCC CCTGGAGGCC 
CACAAATCGA ATACACTGAC ATACCACATG GTCGGCGTGG ATGTGGGCTT CGATAATCCC 
ATGTTCGCCT GCCTAGAGAT CGACTACGAA GAGGCCGACA TGGATCCATC GGGTGACGCC 
GCCCAGCGCA CTCAGCAAAC GCTGACTTTC TACGAGTTGG ATCTCGGCTT AAACCACGTG 
GTTCGCAAAT ACTCGGAGCC CTTGGAAGAG CACGCCAATT TCTTGGTATC CGTGCCTGGG 
GGCAACGATG GTCCTTCGGG CGTGCTGATC TGCTCGGAGA ACTACCTAAC GTACAAGAAT 
CTCGGCGATC AGCACGATAT CCGCTGTCCC ATTCCGCGCA GGAGAAACGA TCTGGATGAT 
CCCGAACGTG GTATGATCTT CATCTGCTCG GCCACACATC GCACCAAGAG CATGTACTTC 
TTCCTGCTGC AGACCGAGCA GGGAGACATC TTTAAGATCA CCCTGGAAAC GGACGATGAT 
GTGGTCTCCG AGATCAAGCT AAAGTACTTC GATACAGTGC CGCCGGCAAC TGCCATGTGC 
GTGCTAAAGA CGGGTTTCCT GTTCGTGGCC AGCGAGTTTG GCAACCACTA TCTATATCAA 
ATCGCTCATC TGGGTGACGA CGATGACGAG CCGGAGTTCA GTTCGGCCAT GCCTTTGGAG 
GAGGGTGAAA CCTTCTTCTT TGCGCCACGT GCCCTAAAGA ACCTGGTGCT TGTGGATGAA 
CTGCCCTCTT TCGCACCGAT CATCACCTCC CAGGTGGCTG ATCTGGCCAA CGAGGACACA 
CCGCAACTGT ACGTTCTTTG CGGACGAGGA CCGCGTTCCA CTCTGCGGGT GCTGCGCCAC 
GGCCTCGAGG TGTCCGAGAT GGCCGTTTCC GAACTGCCTG GTAATCCCAA TGCGGTTTGG 
ACTGTGAAGA AACGAGCTGA TGACGAGTTT GATGCCTATA TCATCGTGTC CTTCGTGAAT 
GCCACCCTGG TTTTGAGCAT TGGCGAAACC GTCGAGGAAG TCACGGACAG CGGTTTCCTG 
GGCACCACGC CCACGCTCTG TTGTGCCGCC CTGGGCGATG ATGCGCTGGT TCAGGTTTAC 
CCCGATGGCA TTCGACATAT CCGATCCGAC AAGCGTGTGA ACGAGTGGAA GGCTCCGGGC 
AAGAAGTCCA TTACCAAGTG CGCCGTGAAT CAGCGCCAGG TGGTCATCAC CTTGTCGGGC 
AGGGAGTTGG TCTACTTCGA AATGGATCCG ACTGGGGAGC TGAACGAGTA CACGGAACGT 
TCCGAGATGC CTGCTGAGAT TATGTGCATG GCCTTGGGAA CTGTTCCGGA GGGCGAGCAG 
AGATCCTGGT TCTTGGCCGT CGGCCTGGCG GATAATACAG TGCGCATCTT ATCGCTGGAT 
CCCAACAACT GTCTCACTCC CTGCTCCATG CAGGCCCTGC CTTCGCCAGC CGAATCCCTT 
TGCCTGGTCG AAATGGGTCA CACGGAGAGC ACGACTCAGG GAGGTTTGGA TGACGATGCT 
CCCGCTCAGC GCAGTGGCAA CAATAAGGGA ACCATTTACC TAAACATTGG CTTGAGCAAC 
GGTGTCCTGC TGAGAACTGT GCTCGATCCT GTTTCCGGAG ATTTGGCAGA CACCCGAACG 
AGATATTTGG GTTCCCGACC CGTAAAGCTC TTCCGGATCA AGATGCAGGG ATCCGAAGCG 
GTGCTGGCCA TGTCCAGTCG AACCTGGCTG TCGTACTACC ACCAAAATCG ATTCCATTTG 
ACGCCTCTGT CTTATGAAAC CCTTGAGTAT GCTTCTGGCT TCTCGAGCGA GCAGTGCAGC 
GAAGGTATCG TGGCTATATC CACCAACACC TTGAGGATCT TGGCCCTGGA GAAACTGGGA 
GCCGTCTTCA ATCAAGTAGC GTTCCCCTTG CAGTACACTC CACGTACCTT TGTAATCCAT 
CCGGATACGG GACGCATGCT AATTGCCGAA ACCGATCACA ACGCCTACAC AGAGGATACG 
AAGAGTGCCC GAAAGGAGCA AATGGCGGAG GAGATGCGCA GTGCAGCTGG CGATGAGGAA 
AGGGAGTTGG CCCGCGAAAT GGCCAATGCT TTCATCAACG AAGTCCTTCC CGAGGACGTG 
TTTTCGTCGC CCAAAGCAGG ACTGGGTCTT TGGGCCTCGC AAATCCGTTG CCTGGACGCA 
ATGCATGGTC AGACGATGTT TAGTGTGCCG CTCACCCAAA ACGAGGCCAT TATGTCGATG 
GCCATGCTGA AGTTCTCCAT AGCGGCTGAT GGCCGGTACT ACCTGGCCGT GGGAATCGCC 
AAGGATCTGC AGCTGAATCC CAGGATTTCG CAAGGCGGCT GCATAGATAT CTACAAAATA 
GATCCCACTT GCTCCAGTCT GGAATTCATG CACCGCACGG ACATTGACGA GATTCCTGGA 
GCCCTGTGTG GCTTCCAGGG TCGTCTACTA GCTGGCTGCG GTCGAATGTT GCGAATCTAC 
GATTTCGGCA AGAAGAAAAT GCTGCGCAAA TGCGAGAACA AGCACATTCC CTATCAGATT 
GTCAATATCC AAGCCATGGG TCATCGTGTT TACGTGTCGG ATGTTCAGGA ATCAGTATTT 
TTCATACGCT ATCGTCGCGC GGAGAACCAG CTGATTATAT TTGCCGATGA CACGCATCCT 
CGATGGGTGA CGGCCACCAC CTTGCTGGAC TATGATACCA TAGCCATAGC CGATAAGTTT 
GGCAACCTGA GCATTCAGCG ATTGCCCCAC TCCGTAACGG ACGATGTGGA CGAGGATCCA 
ACGGGCACCA AGTCTCTGTG GGATCGTGGC CTGCTGTCAG GTGCTTCACA GAAGTCGGAG 
AACATTTGCT CCTTCCATGT GGGCGAGATA ATCATGTCGC TGCAGAAGGC AACGCTAATT 
CCTGGAGGAT CGGAAGCCCT TATATACGCC ACGCTAAGTG GCACTGTTGG AGCATTTGTT 
CCGTTCACCA GTCGCGAGGA CTACGACTTC TTCCAACATC TGGAGATGCA CATGCGCAAT 
GAGAATCCTC CACTCTGTGG ACGCGACCAT CTCAGCTACA GGAGCTCGTA TTATCCGGTC 
AAAAACGTAC TCGACGGCGA TCTCTGCGAG CAGTATCTGT CCATTGAGGC AGCCAAGCAG 
AAGAGCATAG CTGGCGACAT GTTCCGCACT CCCAATCAGA TCTGCAAGAA ACTGGAGGAT 
ATTCGCACGC GATACGCCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728546.1
CDS	162..3845
Translation	MYLYNLTLQKATGVTHAVHGNFSGGKQQEVLLSRGKSLELLRPDSNTGKVHTLLSTEIFGCVRALMAFRLTGGTKDYIVVGSDSGRIVILEYNPSKNALEKVHQETFGKSGCRRIVPGQYFAIDPKGRAVMIGAVEKQKLAYIMNRDTQARLTISSPLEAHKSNTLTYHMVGVDVGFDNPMFACLEIDYEEADMDPSGDAAQRTQQTLTFYELDLGLNHVVRKYSEPLEEHANFLVSVPGGNDGPSGVLICSENYLTYKNLGDQHDIRCPIPRRRNDLDDPERGMIFICSATHRTKSMYFFLLQTEQGDIFKITLETDDDVVSEIKLKYFDTVPPATAMCVLKTGFLFVASEFGNHYLYQIAHLGDDDDEPEFSSAMPLEEGETFFFAPRALKNLVLVDELPSFAPIITSQVADLANEDTPQLYVLCGRGPRSTLRVLRHGLEVSEMAVSELPGNPNAVWTVKKRADDEFDAYIIVSFVNATLVLSIGETVEEVTDSGFLGTTPTLCCAALGDDALVQVYPDGIRHIRSDKRVNEWKAPGKKSITKCAVNQRQVVITLSGRELVYFEMDPTGELNEYTERSEMPAEIMCMALGTVPEGEQRSWFLAVGLADNTVRILSLDPNNCLTPCSMQALPSPAESLCLVEMGHTESTTQGGLDDDAPAQRSGNNKGTIYLNIGLSNGVLLRTVLDPVSGDLADTRTRYLGSRPVKLFRIKMQGSEAVLAMSSRTWLSYYHQNRFHLTPLSYETLEYASGFSSEQCSEGIVAISTNTLRILALEKLGAVFNQVAFPLQYTPRTFVIHPDTGRMLIAETDHNAYTEDTKSARKEQMAEEMRSAAGDEERELAREMANAFINEVLPEDVFSSPKAGLGLWASQIRCLDAMHGQTMFSVPLTQNEAIMSMAMLKFSIAADGRYYLAVGIAKDLQLNPRISQGGCIDIYKIDPTCSSLEFMHRTDIDEIPGALCGFQGRLLAGCGRMLRIYDFGKKKMLRKCENKHIPYQIVNIQAMGHRVYVSDVQESVFFIRYRRAENQLIIFADDTHPRWVTATTLLDYDTIAIADKFGNLSIQRLPHSVTDDVDEDPTGTKSLWDRGLLSGASQKSENICSFHVGEIIMSLQKATLIPGGSEALIYATLSGTVGAFVPFTSREDYDFFQHLEMHMRNENPPLCGRDHLSYRSSYYPVKNVLDGDLCEQYLSIEAAKQKSIAGDMFRTPNQICKKLEDIRTRYAF

Target ORF information:

RefSeq Version	NM_167842.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster splicing factor 3b subunit 3, transcript variant A (Sf3b3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167842.3

ORF Insert Sequence:

ATGTATCTGT ACAACCTGAC CCTGCAGAAG GCCACCGGCG TGACCCACGC GGTCCACGGC 
AACTTCTCCG GCGGCAAGCA GCAGGAGGTC CTCCTGTCGC GTGGCAAGTC GCTGGAGCTA 
CTGCGTCCGG ACTCCAACAC CGGCAAGGTG CACACCCTGC TCTCCACGGA GATATTCGGA 
TGCGTTCGCG CCCTGATGGC CTTTCGACTG ACGGGCGGCA CTAAAGATTA CATAGTTGTG 
GGCTCGGACT CGGGACGCAT CGTGATCCTC GAGTACAATC CCTCCAAGAA TGCTCTTGAA 
AAAGTGCACC AGGAGACGTT CGGAAAGTCG GGATGTCGCA GGATTGTGCC TGGGCAATAC 
TTTGCCATCG ATCCTAAAGG AAGAGCCGTG ATGATTGGCG CCGTGGAGAA GCAAAAGCTG 
GCCTACATCA TGAACCGGGA CACGCAGGCT CGCCTCACGA TCTCATCGCC CCTGGAGGCC 
CACAAATCGA ATACACTGAC ATACCACATG GTCGGCGTGG ATGTGGGCTT CGATAATCCC 
ATGTTCGCCT GCCTAGAGAT CGACTACGAA GAGGCCGACA TGGATCCATC GGGTGACGCC 
GCCCAGCGCA CTCAGCAAAC GCTGACTTTC TACGAGTTGG ATCTCGGCTT AAACCACGTG 
GTTCGCAAAT ACTCGGAGCC CTTGGAAGAG CACGCCAATT TCTTGGTATC CGTGCCTGGG 
GGCAACGATG GTCCTTCGGG CGTGCTGATC TGCTCGGAGA ACTACCTAAC GTACAAGAAT 
CTCGGCGATC AGCACGATAT CCGCTGTCCC ATTCCGCGCA GGAGAAACGA TCTGGATGAT 
CCCGAACGTG GTATGATCTT CATCTGCTCG GCCACACATC GCACCAAGAG CATGTACTTC 
TTCCTGCTGC AGACCGAGCA GGGAGACATC TTTAAGATCA CCCTGGAAAC GGACGATGAT 
GTGGTCTCCG AGATCAAGCT AAAGTACTTC GATACAGTGC CGCCGGCAAC TGCCATGTGC 
GTGCTAAAGA CGGGTTTCCT GTTCGTGGCC AGCGAGTTTG GCAACCACTA TCTATATCAA 
ATCGCTCATC TGGGTGACGA CGATGACGAG CCGGAGTTCA GTTCGGCCAT GCCTTTGGAG 
GAGGGTGAAA CCTTCTTCTT TGCGCCACGT GCCCTAAAGA ACCTGGTGCT TGTGGATGAA 
CTGCCCTCTT TCGCACCGAT CATCACCTCC CAGGTGGCTG ATCTGGCCAA CGAGGACACA 
CCGCAACTGT ACGTTCTTTG CGGACGAGGA CCGCGTTCCA CTCTGCGGGT GCTGCGCCAC 
GGCCTCGAGG TGTCCGAGAT GGCCGTTTCC GAACTGCCTG GTAATCCCAA TGCGGTTTGG 
ACTGTGAAGA AACGAGCTGA TGACGAGTTT GATGCCTATA TCATCGTGTC CTTCGTGAAT 
GCCACCCTGG TTTTGAGCAT TGGCGAAACC GTCGAGGAAG TCACGGACAG CGGTTTCCTG 
GGCACCACGC CCACGCTCTG TTGTGCCGCC CTGGGCGATG ATGCGCTGGT TCAGGTTTAC 
CCCGATGGCA TTCGACATAT CCGATCCGAC AAGCGTGTGA ACGAGTGGAA GGCTCCGGGC 
AAGAAGTCCA TTACCAAGTG CGCCGTGAAT CAGCGCCAGG TGGTCATCAC CTTGTCGGGC 
AGGGAGTTGG TCTACTTCGA AATGGATCCG ACTGGGGAGC TGAACGAGTA CACGGAACGT 
TCCGAGATGC CTGCTGAGAT TATGTGCATG GCCTTGGGAA CTGTTCCGGA GGGCGAGCAG 
AGATCCTGGT TCTTGGCCGT CGGCCTGGCG GATAATACAG TGCGCATCTT ATCGCTGGAT 
CCCAACAACT GTCTCACTCC CTGCTCCATG CAGGCCCTGC CTTCGCCAGC CGAATCCCTT 
TGCCTGGTCG AAATGGGTCA CACGGAGAGC ACGACTCAGG GAGGTTTGGA TGACGATGCT 
CCCGCTCAGC GCAGTGGCAA CAATAAGGGA ACCATTTACC TAAACATTGG CTTGAGCAAC 
GGTGTCCTGC TGAGAACTGT GCTCGATCCT GTTTCCGGAG ATTTGGCAGA CACCCGAACG 
AGATATTTGG GTTCCCGACC CGTAAAGCTC TTCCGGATCA AGATGCAGGG ATCCGAAGCG 
GTGCTGGCCA TGTCCAGTCG AACCTGGCTG TCGTACTACC ACCAAAATCG ATTCCATTTG 
ACGCCTCTGT CTTATGAAAC CCTTGAGTAT GCTTCTGGCT TCTCGAGCGA GCAGTGCAGC 
GAAGGTATCG TGGCTATATC CACCAACACC TTGAGGATCT TGGCCCTGGA GAAACTGGGA 
GCCGTCTTCA ATCAAGTAGC GTTCCCCTTG CAGTACACTC CACGTACCTT TGTAATCCAT 
CCGGATACGG GACGCATGCT AATTGCCGAA ACCGATCACA ACGCCTACAC AGAGGATACG 
AAGAGTGCCC GAAAGGAGCA AATGGCGGAG GAGATGCGCA GTGCAGCTGG CGATGAGGAA 
AGGGAGTTGG CCCGCGAAAT GGCCAATGCT TTCATCAACG AAGTCCTTCC CGAGGACGTG 
TTTTCGTCGC CCAAAGCAGG ACTGGGTCTT TGGGCCTCGC AAATCCGTTG CCTGGACGCA 
ATGCATGGTC AGACGATGTT TAGTGTGCCG CTCACCCAAA ACGAGGCCAT TATGTCGATG 
GCCATGCTGA AGTTCTCCAT AGCGGCTGAT GGCCGGTACT ACCTGGCCGT GGGAATCGCC 
AAGGATCTGC AGCTGAATCC CAGGATTTCG CAAGGCGGCT GCATAGATAT CTACAAAATA 
GATCCCACTT GCTCCAGTCT GGAATTCATG CACCGCACGG ACATTGACGA GATTCCTGGA 
GCCCTGTGTG GCTTCCAGGG TCGTCTACTA GCTGGCTGCG GTCGAATGTT GCGAATCTAC 
GATTTCGGCA AGAAGAAAAT GCTGCGCAAA TGCGAGAACA AGCACATTCC CTATCAGATT 
GTCAATATCC AAGCCATGGG TCATCGTGTT TACGTGTCGG ATGTTCAGGA ATCAGTATTT 
TTCATACGCT ATCGTCGCGC GGAGAACCAG CTGATTATAT TTGCCGATGA CACGCATCCT 
CGATGGGTGA CGGCCACCAC CTTGCTGGAC TATGATACCA TAGCCATAGC CGATAAGTTT 
GGCAACCTGA GCATTCAGCG ATTGCCCCAC TCCGTAACGG ACGATGTGGA CGAGGATCCA 
ACGGGCACCA AGTCTCTGTG GGATCGTGGC CTGCTGTCAG GTGCTTCACA GAAGTCGGAG 
AACATTTGCT CCTTCCATGT GGGCGAGATA ATCATGTCGC TGCAGAAGGC AACGCTAATT 
CCTGGAGGAT CGGAAGCCCT TATATACGCC ACGCTAAGTG GCACTGTTGG AGCATTTGTT 
CCGTTCACCA GTCGCGAGGA CTACGACTTC TTCCAACATC TGGAGATGCA CATGCGCAAT 
GAGAATCCTC CACTCTGTGG ACGCGACCAT CTCAGCTACA GGAGCTCGTA TTATCCGGTC 
AAAAACGTAC TCGACGGCGA TCTCTGCGAG CAGTATCTGT CCATTGAGGC AGCCAAGCAG 
AAGAGCATAG CTGGCGACAT GTTCCGCACT CCCAATCAGA TCTGCAAGAA ACTGGAGGAT 
ATTCGCACGC GATACGCCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Sf3b3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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