emc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	emc
Entrez Gene ID	38091
Full Name	extra macrochaetae
Synonyms	0094/26,0203/10,0587/01,0977/09,Ach,CG1007,Dm0688,Dmel\CG1007,Emc,bHLHb28,gov,l(3)04322,l(3)05592,l(3)61Da,l(3)j4E11,ms(3)61CD
General protein information	Preferred Names extra macrochaetae Names CG1007 gene product from transcript CG1007-RA CG1007-PA achetous emc-PA extra macrochaete extra macrocheatae extramachrochaetae extramacrochaetae extramacrochaete extramacrocheate golovan male sterile(3)61CD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 61C9-61C9\|3-0.0 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_079152.4	NP_523876.2	extra macrochaetae

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Notch signaling activates the expression of extramacrochaetae (emc), which encodes a helix-loop-helix (HLH) transcription factor. Emc, in turn, then forms a biochemical complex with Da.
Title: Inhibition of Daughterless by Extramacrochaetae mediates Notch-induced cell proliferation.

Extramacrochaetae functions in dorsal-ventral patterning of Drosophila imaginal discs.
Title: Extramacrochaetae functions in dorsal-ventral patterning of Drosophila imaginal discs.

argue that the role emc, h and sr in development of the flight apparatus preceded their activities for macrochaete patterning
Title: The bristle patterning genes hairy and extramacrochaetae regulate the development of structures required for flight in Diptera.

we show that during the development of epithelial cells in the imaginal discs, the G2/M transition, and hence cell proliferation, is controlled by Emc via Da. In eukaryotic cells, the main activator of this transition is the Cdc25 phosphatase, string
Title: The bHLH factors extramacrochaetae and daughterless control cell cycle in Drosophila imaginal discs through the transcriptional regulation of the Cdc25 phosphatase string.

Hh drives and Emc impedes the progression of the furrow across the developing retina.
Title: Hedgehog and extramacrochaetae in the Drosophila eye: an irresistible force meets an immovable object.

The following emc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the emc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa20384	NM_079152.4 Latest version!	Drosophila melanogaster extra macrochaetae (emc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

emc ( NM_079152.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523876.2 Drosophila melanogaster extra macrochaetae (emc), mRNA.

Price & Availability↑

CloneID	OFa20384
Clone ID Related Accession (Same CDS sequence)	NM_079152.4
Accession Version	NM_079152.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	extra macrochaetae
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079152.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGTCCC TGACGGCCGT CTGCCAGACA GGTGCCTCCG GAATGCCGGC CTTGAATGCC 
AGCGGGCGCA TCCAGCGCCA TCCCACGCAC CGCGGCGACG GGGAGAACGC CGAGATGAAG 
ATGTATCTGT CCAAACTGAA GGACCTCGTT CCGTTCATGC CCAAGAACAG GAAGCTCACC 
AAGCTGGAGA TCATCCAGCA CGTCATCGAC TACATCTGCG ACCTGCAGAC CGAGCTGGAG 
ACGCACCCCG AGATGGGCAA CTTCGATGCG GCAGCCGCTC TGACGGCGGT GAACGGACTC 
CACGAGGACG AGGACAGCGA CATGGAGGAT GCGGATGCCG AGGCAGAAGC GGAAGTCGAT 
CCAGATATCC TCGCCCAGCG CCTGAATGCC GAGCAGCCGG CGAAAGTCTC TAGTCCCGCC 
GCCCGTCTCC CGCTTACCGA TCGCCAAACG CCCAACACTC TTGTGGCGCC CGCCCATCCG 
CAGCAGCATC AGCAGCAGCA GCAACTGCAA CTGCAGCAGC AACAACTGCA ATCACAGCAG 
CAACTGTCCA ACAGTTTAGC AACGCCACAG AATGCGGAGA AAGACAGCAG ACAGTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523876.2
CDS	265..864
Translation	MKSLTAVCQTGASGMPALNASGRIQRHPTHRGDGENAEMKMYLSKLKDLVPFMPKNRKLTKLEIIQHVIDYICDLQTELETHPEMGNFDAAAALTAVNGLHEDEDSDMEDADAEAEAEVDPDILAQRLNAEQPAKVSSPAARLPLTDRQTPNTLVAPAHPQQHQQQQQLQLQQQQLQSQQQLSNSLATPQNAEKDSRQS

Target ORF information:

RefSeq Version	NM_079152.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster extra macrochaetae (emc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079152.4

ORF Insert Sequence:

ATGAAGTCCC TGACGGCCGT CTGCCAGACA GGTGCCTCCG GAATGCCGGC CTTGAATGCC 
AGCGGGCGCA TCCAGCGCCA TCCCACGCAC CGCGGCGACG GGGAGAACGC CGAGATGAAG 
ATGTATCTGT CCAAACTGAA GGACCTCGTT CCGTTCATGC CCAAGAACAG GAAGCTCACC 
AAGCTGGAGA TCATCCAGCA CGTCATCGAC TACATCTGCG ACCTGCAGAC CGAGCTGGAG 
ACGCACCCCG AGATGGGCAA CTTCGATGCG GCAGCCGCTC TGACGGCGGT GAACGGACTC 
CACGAGGACG AGGACAGCGA CATGGAGGAT GCGGATGCCG AGGCAGAAGC GGAAGTCGAT 
CCAGATATCC TCGCCCAGCG CCTGAATGCC GAGCAGCCGG CGAAAGTCTC TAGTCCCGCC 
GCCCGTCTCC CGCTTACCGA TCGCCAAACG CCCAACACTC TTGTGGCGCC CGCCCATCCG 
CAGCAGCATC AGCAGCAGCA GCAACTGCAA CTGCAGCAGC AACAACTGCA ATCACAGCAG 
CAACTGTCCA ACAGTTTAGC AACGCCACAG AATGCGGAGA AAGACAGCAG ACAGTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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emc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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