trh cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	trh
Entrez Gene ID	38065
Full Name	trachealess
Synonyms	3.1,BP1081,CG13885,CG42865,CG6883,DMU42699,Dmel\CG42865,Dmel_CG13885,Dmel_CG6883,HDC07747,TRH,Trh,bHLHe13,l(3)10512
General protein information	Preferred Names trachealess Names CG42865 gene product from transcript CG42865-RF CG42865-PA CG42865-PB CG42865-PC CG42865-PD CG42865-PE CG42865-PF CG42865-PG trh-PA trh-PB trh-PC trh-PD trh-PE trh-PF trh-PG
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 61C1-61C1\|3-1 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274279.1	NP_001261208.1	trachealess, isoform E
NM_001103991.2	NP_001097461.2	trachealess, isoform C
NM_001043110.2	NP_001036575.1	trachealess, isoform B
NM_079148.3	NP_523872.2	trachealess, isoform A
NM_001274281.1	NP_001261210.1	trachealess, isoform G
NM_001274278.1	NP_001261207.1	trachealess, isoform D
NM_001274280.1	NP_001261209.1	trachealess, isoform F

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	trh	NP_001097461.2
Caenorhabditis elegans (roundworm)	hlh-34	NP_506391.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

expression of every tracheal gene we tested was dependent on Trh, suggesting a major role for Trh in activation and maintenance of tracheal gene expression
Title: Trachealess (Trh) regulates all tracheal genes during Drosophila embryogenesis.

Study shows that jing is a direct downstream target of Trh/Tgo and that Vvl and Pnt are also involved in jing tracheal activation.
Title: The Drosophila jing gene is a downstream target in the Trachealess/Tango tracheal pathway.

trachealess and tango, both encoding bHLH-PAS proteins that are required for the formation of the embryonic tracheal system, are essential for forming two adjacent distal segments of the leg
Title: Fate determination of Drosophila leg distal regions by trachealess and tango through repression and stimulation, respectively, of Bar homeobox gene expression in the future pretarsus and tarsus.

Activity of crossveinless-c, a gene coding for a RhoGAP and whose specific transcriptional activation in the tracheal cells is triggered by both the trachealess patterning gene and the EGF Receptor (EGFR) signaling pathway.
Title: The RhoGAP crossveinless-c links trachealess and EGFR signaling to cell shape remodeling in Drosophila tracheal invagination.

competitive interactions between basic helix-loop-helix-PAS proteins (Dysfusion, Trachealess, and possibly Similar) may be important for the proper development of the trachea
Title: The Drosophila dysfusion basic helix-loop-helix (bHLH)-PAS gene controls tracheal fusion and levels of the trachealess bHLH-PAS protein.

The following trh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17069	NM_001274280.1 Latest version!	Drosophila melanogaster trachealess (trh), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa17070	NM_001274281.1 Latest version!	Drosophila melanogaster trachealess (trh), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa17071	NM_001274279.1 Latest version!	Drosophila melanogaster trachealess (trh), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa20352	NM_001103991.2 Latest version!	Drosophila melanogaster trachealess (trh), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa20354	NM_001043110.2 Latest version!	Drosophila melanogaster trachealess (trh), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa20355	NM_079148.3 Latest version!	Drosophila melanogaster trachealess (trh), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa17072	NM_001274278.1 Latest version!	Drosophila melanogaster trachealess (trh), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

trh ( NM_001274280.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261209.1 Drosophila melanogaster trachealess (trh), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa17069
Clone ID Related Accession (Same CDS sequence)	NM_001274280.1
Accession Version	NM_001274280.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2970bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	trachealess, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCATC ACGGCAGTGG GTTCGTGGCT TCGCCATGGG CCGCCGTTCT GGGACACCAC 
TCGATGGCCT CCGATGCCGG GTTCGCCGCT GCGGCTGCTG CGGCGCATGT CCAGAACCAC 
TCGATGCACC ATCCTATACA CTCGCATCAC CACCACCACT CGCACTCGCA TCCACACCCA 
CATCCGCACT CGCATCCGCA CCACCATCCG CACCTGGGAA CCGCCGGAGG GATGCCGATG 
GATCTGCATG TGCCGCAGGG ATTCCCCTAC TACAGATACC GAGAGGACGC ACTGTGCTGG 
GGCGACAGAA AGTCCATGGA GGAGATCGGA GCGGCCCAGT CCTCGGTGAA TGCGCGCATC 
CTGGAGCTGC GCAAGGAGAA GTCGAGGGAC GCGGCGAGAT CGCGGCGCGG AAAGGAGAAC 
TACGAGTTCT ACGAGCTAGC CAAGATGCTC CCTCTGCCGG CAGCCATCAC CAGCCAGCTG 
GACAAGGCCT CGATCATAAG ACTGACCATC AGCTACCTGA AGCTGAGGGA TTTCTCCGGA 
CACGGCGATC CGCCATGGAC TCGGGAAGCC TCTAGCAGCA GTAAGCTAAA AAGTGCCGCC 
ATTCGTCGCA GCCCCGCTGT TGATTTGTTC GAGCAACATC AGGGCACCCA CATATTACAG 
TCGCTGGATG GTTTCGCCCT AGCTGTGGCG GCAGACGGAC GCTTCCTGTA TATTTCCGAG 
ACGGTGTCCA TCTATTTGGG CCTGTCGCAG GTGGAGATGA CGGGTAGCAG CATCTTCGAC 
TATATCCACC AGGCGGATCA CTCGGAGATC GCCGACCAGC TGGGCCTGAG CCTAACAAGC 
GGCGGCGGCG GTGGCGGTGG AAGCTCGAGC AGCGGCGGAG GAGGAGGCGG AGCGGGAGGT 
GGTATGGCCT CACCCACTTC CGGAGCCTCC GACGATGGAA GCGGCACACA CGTTGCGGCC 
TCCATGACCC AAGCTTCGAC CAGCGGCTAC AAGGGATACG ATCGAAGCTT CTGTGTCCGT 
ATGAAGTCCA CGCTGACCAA GCGCGGCTGT CACTTCAAAT CCTCAGGCTA TCGGGCCAGC 
GATGCAACGA GCAATTGCAA CAACGGTAAC AATGCTAGTA ACAATGCTAA AAACGTTAAG 
AATCCGGGCT CAAACTATTC GGTGGTTCTG CTGTTGTGCA AGCTGCGTCC CCAGTACACA 
TTTTCCCACA GTCGCAAATC GCAGCCTCCA CTCCTCGGAA TGGTTGCCCT GGCCATCGCA 
CTGCCTCCGC CATCGGTGCA CGAGATCCGG CTGGAGTGCG ACATGTTCGT CACCAGGGTG 
AACTTTGACC TGCGAGTAGC CCACTGCGAG CCAAGGGTAT CCGATCTGCT GGACTATTCC 
CCGGAGGACC TGGTTAACAA GAGCTTGTAC TCACTGTGTC ACGCCGAGGA TGCCAATCGC 
CTGCGCAAGA GCCACTCAGA CCTGATCGAG AAGGGTCAGG TGTTGACCGG ATACTACCGA 
CTAATGAACA AGAGTGGAGG CTACACTTGG CTCCAGACCT GTGCCACTGT CGTCTGCAGC 
ACAAAGAACG CCGATGAGCA GAACATCATC TGCGTGAACT ATGTGATCAG CAACCGGGAG 
AACGAGAACA TGATACTGGA TTGCTGCCAA CTGGAACCCA GTCCGGATAG CATTAAGCAC 
GAGGAGGGAC TGGGTAACGA CAAAAGCAGC GGGTCGCCAG GAGGAGATGC CAGTGGCGAA 
GGCAACTCTC ACCTGAGTGC CGGAGACATG AAGCTTAACT CACCGAAAAC CGATTCAGAG 
GGCCATTCCC ACCGCGGCAG GGGTCGCAGT GCAGCCGCCT CGCATGGAAG TTCAATGAAC 
AGCCTCACCA TGATCAAGGA TAGCCCCACT CCGCTGGGTG TGGAGATCGA TTCGGGAGTG 
CTGCCCACCA CAGTGGCCAC TCCAGTGCCG GCAGCCACTC CACCAGTGCA GTCCACCAAG 
CGAAAGCGAA AAACCAAAGC GTCGCAGCAC GCGGAGGACC AGGGTCAAGA GCAGGTAATC 
TCCGAGCAGC CTCTGCCCAA ACTACCGACA ATGGAGCAAC GCGATCAGCA GCCCCGCAGC 
CGCTTACCCT CGATTGTGGA TGAGCAGCCC TCGTCCGCGG CCGACTCCGC GGTCAAGGAT 
CTGGAGCAGG CCATGTCCAA GCATCTGCCC TCGCCAGCGG CGGTGGTGTC CGTGGCCCCG 
CCCAATACGG ATTTCAGTGC CGACTCTCTG CTTAAGCAGC AGCAACAACA ACAGCAGCTG 
GATCCCAACG AGAAAAGCAG CACCATTCAA TGGATAGGCA CTCCCTACCA ACAGCCACCG 
GCGCCCATGC CAGCTACCGC CCTTCTCCGG CAGTTATACG CCAACCGGGA GAGCGTGATC 
CGGGCGACGG CTAGGCAAAC ACCAACTGGA GTGGGCCCCG GAGTTTTCTA CGGCGATCAG 
CAAACAGGTC CGCTGCCCAC TCCGCCGGGC AGTGAGTCGT CCTACGAGAA CCAATACCTG 
CAGCTGCACT CTGCCGCTTC CGGTGGACAT CCTGGCGGCC AGAAGACCTC CGCTGATGCC 
TTCACCAATC TGGTGTCAAC CTACGGCGGC TATCACAGCT CCATTGACTA TCACAACGCC 
ATGACCCCGC CCAGTTCAGT GTCTCCCAGG GACTCGAATC AACCGGGTAA GGCGGCTCCA 
GTCCTGGCCT CCAACGGGGG ATACGACTAT GCCCCCGATC CACTCCGAGG GCAGTACGCC 
ACTTCATCCG GAGACGTGGT GCCCGCCACG CTGCCTCTGA AGCCCCAGGC ATCCTATACA 
GCAACAATGC ATCCTTCGGG CAGCACAACC ACCGAGGGTG GAGTTACCTA CAGTAATCTC 
GACCAGCCGC AGTACTTCGC TCCGCATTCG AGTTTCCACC TTTACCACAA GGGCAGCCCG 
GCCAGCGGCT GGTACTCCAC GCCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261209.1
CDS	559..3528
Translation	MEHHGSGFVASPWAAVLGHHSMASDAGFAAAAAAAHVQNHSMHHPIHSHHHHHSHSHPHPHPHSHPHHHPHLGTAGGMPMDLHVPQGFPYYRYREDALCWGDRKSMEEIGAAQSSVNARILELRKEKSRDAARSRRGKENYEFYELAKMLPLPAAITSQLDKASIIRLTISYLKLRDFSGHGDPPWTREASSSSKLKSAAIRRSPAVDLFEQHQGTHILQSLDGFALAVAADGRFLYISETVSIYLGLSQVEMTGSSIFDYIHQADHSEIADQLGLSLTSGGGGGGGSSSSGGGGGGAGGGMASPTSGASDDGSGTHVAASMTQASTSGYKGYDRSFCVRMKSTLTKRGCHFKSSGYRASDATSNCNNGNNASNNAKNVKNPGSNYSVVLLLCKLRPQYTFSHSRKSQPPLLGMVALAIALPPPSVHEIRLECDMFVTRVNFDLRVAHCEPRVSDLLDYSPEDLVNKSLYSLCHAEDANRLRKSHSDLIEKGQVLTGYYRLMNKSGGYTWLQTCATVVCSTKNADEQNIICVNYVISNRENENMILDCCQLEPSPDSIKHEEGLGNDKSSGSPGGDASGEGNSHLSAGDMKLNSPKTDSEGHSHRGRGRSAAASHGSSMNSLTMIKDSPTPLGVEIDSGVLPTTVATPVPAATPPVQSTKRKRKTKASQHAEDQGQEQVISEQPLPKLPTMEQRDQQPRSRLPSIVDEQPSSAADSAVKDLEQAMSKHLPSPAAVVSVAPPNTDFSADSLLKQQQQQQQLDPNEKSSTIQWIGTPYQQPPAPMPATALLRQLYANRESVIRATARQTPTGVGPGVFYGDQQTGPLPTPPGSESSYENQYLQLHSAASGGHPGGQKTSADAFTNLVSTYGGYHSSIDYHNAMTPPSSVSPRDSNQPGKAAPVLASNGGYDYAPDPLRGQYATSSGDVVPATLPLKPQASYTATMHPSGSTTTEGGVTYSNLDQPQYFAPHSSFHLYHKGSPASGWYSTPS

Target ORF information:

RefSeq Version	NM_001274280.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster trachealess (trh), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274280.1

ORF Insert Sequence:

ATGGAGCATC ACGGCAGTGG GTTCGTGGCT TCGCCATGGG CCGCCGTTCT GGGACACCAC 
TCGATGGCCT CCGATGCCGG GTTCGCCGCT GCGGCTGCTG CGGCGCATGT CCAGAACCAC 
TCGATGCACC ATCCTATACA CTCGCATCAC CACCACCACT CGCACTCGCA TCCACACCCA 
CATCCGCACT CGCATCCGCA CCACCATCCG CACCTGGGAA CCGCCGGAGG GATGCCGATG 
GATCTGCATG TGCCGCAGGG ATTCCCCTAC TACAGATACC GAGAGGACGC ACTGTGCTGG 
GGCGACAGAA AGTCCATGGA GGAGATCGGA GCGGCCCAGT CCTCGGTGAA TGCGCGCATC 
CTGGAGCTGC GCAAGGAGAA GTCGAGGGAC GCGGCGAGAT CGCGGCGCGG AAAGGAGAAC 
TACGAGTTCT ACGAGCTAGC CAAGATGCTC CCTCTGCCGG CAGCCATCAC CAGCCAGCTG 
GACAAGGCCT CGATCATAAG ACTGACCATC AGCTACCTGA AGCTGAGGGA TTTCTCCGGA 
CACGGCGATC CGCCATGGAC TCGGGAAGCC TCTAGCAGCA GTAAGCTAAA AAGTGCCGCC 
ATTCGTCGCA GCCCCGCTGT TGATTTGTTC GAGCAACATC AGGGCACCCA CATATTACAG 
TCGCTGGATG GTTTCGCCCT AGCTGTGGCG GCAGACGGAC GCTTCCTGTA TATTTCCGAG 
ACGGTGTCCA TCTATTTGGG CCTGTCGCAG GTGGAGATGA CGGGTAGCAG CATCTTCGAC 
TATATCCACC AGGCGGATCA CTCGGAGATC GCCGACCAGC TGGGCCTGAG CCTAACAAGC 
GGCGGCGGCG GTGGCGGTGG AAGCTCGAGC AGCGGCGGAG GAGGAGGCGG AGCGGGAGGT 
GGTATGGCCT CACCCACTTC CGGAGCCTCC GACGATGGAA GCGGCACACA CGTTGCGGCC 
TCCATGACCC AAGCTTCGAC CAGCGGCTAC AAGGGATACG ATCGAAGCTT CTGTGTCCGT 
ATGAAGTCCA CGCTGACCAA GCGCGGCTGT CACTTCAAAT CCTCAGGCTA TCGGGCCAGC 
GATGCAACGA GCAATTGCAA CAACGGTAAC AATGCTAGTA ACAATGCTAA AAACGTTAAG 
AATCCGGGCT CAAACTATTC GGTGGTTCTG CTGTTGTGCA AGCTGCGTCC CCAGTACACA 
TTTTCCCACA GTCGCAAATC GCAGCCTCCA CTCCTCGGAA TGGTTGCCCT GGCCATCGCA 
CTGCCTCCGC CATCGGTGCA CGAGATCCGG CTGGAGTGCG ACATGTTCGT CACCAGGGTG 
AACTTTGACC TGCGAGTAGC CCACTGCGAG CCAAGGGTAT CCGATCTGCT GGACTATTCC 
CCGGAGGACC TGGTTAACAA GAGCTTGTAC TCACTGTGTC ACGCCGAGGA TGCCAATCGC 
CTGCGCAAGA GCCACTCAGA CCTGATCGAG AAGGGTCAGG TGTTGACCGG ATACTACCGA 
CTAATGAACA AGAGTGGAGG CTACACTTGG CTCCAGACCT GTGCCACTGT CGTCTGCAGC 
ACAAAGAACG CCGATGAGCA GAACATCATC TGCGTGAACT ATGTGATCAG CAACCGGGAG 
AACGAGAACA TGATACTGGA TTGCTGCCAA CTGGAACCCA GTCCGGATAG CATTAAGCAC 
GAGGAGGGAC TGGGTAACGA CAAAAGCAGC GGGTCGCCAG GAGGAGATGC CAGTGGCGAA 
GGCAACTCTC ACCTGAGTGC CGGAGACATG AAGCTTAACT CACCGAAAAC CGATTCAGAG 
GGCCATTCCC ACCGCGGCAG GGGTCGCAGT GCAGCCGCCT CGCATGGAAG TTCAATGAAC 
AGCCTCACCA TGATCAAGGA TAGCCCCACT CCGCTGGGTG TGGAGATCGA TTCGGGAGTG 
CTGCCCACCA CAGTGGCCAC TCCAGTGCCG GCAGCCACTC CACCAGTGCA GTCCACCAAG 
CGAAAGCGAA AAACCAAAGC GTCGCAGCAC GCGGAGGACC AGGGTCAAGA GCAGGTAATC 
TCCGAGCAGC CTCTGCCCAA ACTACCGACA ATGGAGCAAC GCGATCAGCA GCCCCGCAGC 
CGCTTACCCT CGATTGTGGA TGAGCAGCCC TCGTCCGCGG CCGACTCCGC GGTCAAGGAT 
CTGGAGCAGG CCATGTCCAA GCATCTGCCC TCGCCAGCGG CGGTGGTGTC CGTGGCCCCG 
CCCAATACGG ATTTCAGTGC CGACTCTCTG CTTAAGCAGC AGCAACAACA ACAGCAGCTG 
GATCCCAACG AGAAAAGCAG CACCATTCAA TGGATAGGCA CTCCCTACCA ACAGCCACCG 
GCGCCCATGC CAGCTACCGC CCTTCTCCGG CAGTTATACG CCAACCGGGA GAGCGTGATC 
CGGGCGACGG CTAGGCAAAC ACCAACTGGA GTGGGCCCCG GAGTTTTCTA CGGCGATCAG 
CAAACAGGTC CGCTGCCCAC TCCGCCGGGC AGTGAGTCGT CCTACGAGAA CCAATACCTG 
CAGCTGCACT CTGCCGCTTC CGGTGGACAT CCTGGCGGCC AGAAGACCTC CGCTGATGCC 
TTCACCAATC TGGTGTCAAC CTACGGCGGC TATCACAGCT CCATTGACTA TCACAACGCC 
ATGACCCCGC CCAGTTCAGT GTCTCCCAGG GACTCGAATC AACCGGGTAA GGCGGCTCCA 
GTCCTGGCCT CCAACGGGGG ATACGACTAT GCCCCCGATC CACTCCGAGG GCAGTACGCC 
ACTTCATCCG GAGACGTGGT GCCCGCCACG CTGCCTCTGA AGCCCCAGGC ATCCTATACA 
GCAACAATGC ATCCTTCGGG CAGCACAACC ACCGAGGGTG GAGTTACCTA CAGTAATCTC 
GACCAGCCGC AGTACTTCGC TCCGCATTCG AGTTTCCACC TTTACCACAA GGGCAGCCCG 
GCCAGCGGCT GGTACTCCAC GCCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trh ( NM_001274281.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261210.1 Drosophila melanogaster trachealess (trh), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa17070
Clone ID Related Accession (Same CDS sequence)	NM_001274281.1
Accession Version	NM_001274281.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	trachealess, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCCGT ACCAGGCAGC CGTGGCCATG GACTACGCCG GATACCAGAG ACAGCCCACG 
CCCGGACATC CCGGAAGCCA CATGGCCACC ATGGGATCTC TGGGCATGCC CGCGGTGCCC 
TTCACCCACA GCTGGATGGT GCCCACGCAG GACCTTTGCG CCATGCCACC CTACAACAAA 
ATGACCGGAC ACCAGCAGCC GCCAGGTGCC GGAATGCACG CCCAGCAACA GCCCCTCGAG 
CCCGGCATCC TGGAGCTGCG CAAGGAGAAG TCGAGGGACG CGGCGAGATC GCGGCGCGGA 
AAGGAGAACT ACGAGTTCTA CGAGCTAGCC AAGATGCTCC CTCTGCCGGC AGCCATCACC 
AGCCAGCTGG ACAAGGCCTC GATCATAAGA CTGACCATCA GCTACCTGAA GCTGAGGGAT 
TTCTCCGGAC ACGGCGATCC GCCATGGACT CGGGAAGCCT CTAGCAGCAG TAAGCTAAAA 
AGTGCCGCCA TTCGTCGCAG CCCCGCTGTT GATTTGTTCG AGCAACATCA GGGCACCCAC 
ATATTACAGT CGCTGGATGG TTTCGCCCTA GCTGTGGCGG CAGACGGACG CTTCCTGTAT 
ATTTCCGAGA CGGTGTCCAT CTATTTGGGC CTGTCGCAGG TGGAGATGAC GGGTAGCAGC 
ATCTTCGACT ATATCCACCA GGCGGATCAC TCGGAGATCG CCGACCAGCT GGGCCTGAGC 
CTAACAAGCG GCGGCGGCGG TGGCGGTGGA AGCTCGAGCA GCGGCGGAGG AGGAGGCGGA 
GCGGGAGGTG GTATGGCCTC ACCCACTTCC GGAGCCTCCG ACGATGGAAG CGGCACACAC 
GTTGCGGCCT CCATGACCCA AGCTTCGACC AGCGGCTACA AGGGATACGA TCGAAGCTTC 
TGTGTCCGTA TGAAGTCCAC GCTGACCAAG CGCGGCTGTC ACTTCAAATC CTCAGGCTAT 
CGGGTGGTTC TGCTGTTGTG CAAGCTGCGT CCCCAGTACA CATTTTCCCA CAGTCGCAAA 
TCGCAGCCTC CACTCCTCGG AATGGTTGCC CTGGCCATCG CACTGCCTCC GCCATCGGTG 
CACGAGATCC GGCTGGAGTG CGACATGTTC GTCACCAGGG TGAACTTTGA CCTGCGAGTA 
GCCCACTGCG AGCCAAGGGT ATCCGATCTG CTGGACTATT CCCCGGAGGA CCTGGTTAAC 
AAGAGCTTGT ACTCACTGTG TCACGCCGAG GATGCCAATC GCCTGCGCAA GAGCCACTCA 
GACCTGATCG AGAAGGGTCA GGTGTTGACC GGATACTACC GACTAATGAA CAAGAGTGGA 
GGCTACACTT GGCTCCAGAC CTGTGCCACT GTCGTCTGCA GCACAAAGAA CGCCGATGAG 
CAGAACATCA TCTGCGTGAA CTATGTGATC AGCAACCGGG AGAACGAGAA CATGATACTG 
GATTGCTGCC AACTGGAACC CAGTCCGGAT AGCATTAAGC ACGAGGAGGG ACTGGGTAAC 
GACAAAAGCA GCGGGTCGCC AGGAGGAGAT GCCAGTGGCG AAGGCAACTC TCACCTGAGT 
GCCGGAGACA TGAAGCTTAA CTCACCGAAA ACCGATTCAG AGGGCCATTC CCACCGCGGC 
AGGGGTCGCA GTGCAGCCGC CTCGCATGGA AGTTCAATGA ACAGCCTCAC CATGATCAAG 
GATAGCCCCA CTCCGCTGGG TGTGGAGATC GATTCGGGAG TGCTGCCCAC CACAGTGGCC 
ACTCCAGTGC CGGCAGCCAC TCCACCAGTG CAGTCCACCA AGCGAAAGCG AAAAACCAAA 
GCGTCGCAGC ACGCGGAGGA CCAGGGTCAA GAGCAGGTAA TCTCCGAGCA GCCTCTGCCC 
AAACTACCGA CAATGGAGCA ACGCGATCAG CAGCCCCGCA GCCGCTTACC CTCGATTGTG 
GATGAGCAGC CCTCGTCCGC GGCCGACTCC GCGGTCAAGG ATCTGGAGCA GGCCATGTCC 
AAGCATCTGC CCTCGCCAGC GGCGGTGGTG TCCGTGGCCC CGCCCAATAC GGATTTCAGT 
GCCGACTCTC TGCTTAAGCA GCAGCAACAA CAACAGCAGC TGGATCCCAA CGAGAAAAGC 
AGCACCATTC AATGGATAGG CACTCCCTAC CAACAGCCAC CGGCGCCCAT GCCAGCTACC 
GCCCTTCTCC GGCAGTTATA CGCCAACCGG GAGAGCGTGA TCCGGGCGAC GGCTAGGCAA 
ACACCAACTG GAGTGGGCCC CGGAGTTTTC TACGGCGATC AGCAAACAGG TCCGCTGCCC 
ACTCCGCCGG GCAGTGAGTC GTCCTACGAG AACCAATACC TGCAGCTGCA CTCTGCCGCT 
TCCGGTGGAC ATCCTGGCGG CCAGAAGACC TCCGCTGATG CCTTCACCAA TCTGGTGTCA 
ACCTACGGCG GCTATCACAG CTCCATTGAC TATCACAACG CCATGACCCC GCCCAGTTCA 
GTGTCTCCCA GGGACTCGAA TCAACCGGGT AAGGCGGCTC CAGTCCTGGC CTCCAACGGG 
GGATACGACT ATGCCCCCGA TCCACTCCGA GGGCAGTACG CCACTTCATC CGGAGACGTG 
GTGCCCGCCA CGCTGCCTCT GAAGCCCCAG GCATCCTATA CAGCAACAAT GCATCCTTCG 
GGCAGCACAA CCACCGAGGG TGGAGTTACC TACAGTAATC TCGACCAGCC GCAGTACTTC 
GCTCCGCATT CGAGTTTCCA CCTTTACCAC AAGGGCAGCC CGGCCAGCGG CTGGTACTCC 
ACGCCCTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261210.1
CDS	654..3425
Translation	MLPYQAAVAMDYAGYQRQPTPGHPGSHMATMGSLGMPAVPFTHSWMVPTQDLCAMPPYNKMTGHQQPPGAGMHAQQQPLEPGILELRKEKSRDAARSRRGKENYEFYELAKMLPLPAAITSQLDKASIIRLTISYLKLRDFSGHGDPPWTREASSSSKLKSAAIRRSPAVDLFEQHQGTHILQSLDGFALAVAADGRFLYISETVSIYLGLSQVEMTGSSIFDYIHQADHSEIADQLGLSLTSGGGGGGGSSSSGGGGGGAGGGMASPTSGASDDGSGTHVAASMTQASTSGYKGYDRSFCVRMKSTLTKRGCHFKSSGYRVVLLLCKLRPQYTFSHSRKSQPPLLGMVALAIALPPPSVHEIRLECDMFVTRVNFDLRVAHCEPRVSDLLDYSPEDLVNKSLYSLCHAEDANRLRKSHSDLIEKGQVLTGYYRLMNKSGGYTWLQTCATVVCSTKNADEQNIICVNYVISNRENENMILDCCQLEPSPDSIKHEEGLGNDKSSGSPGGDASGEGNSHLSAGDMKLNSPKTDSEGHSHRGRGRSAAASHGSSMNSLTMIKDSPTPLGVEIDSGVLPTTVATPVPAATPPVQSTKRKRKTKASQHAEDQGQEQVISEQPLPKLPTMEQRDQQPRSRLPSIVDEQPSSAADSAVKDLEQAMSKHLPSPAAVVSVAPPNTDFSADSLLKQQQQQQQLDPNEKSSTIQWIGTPYQQPPAPMPATALLRQLYANRESVIRATARQTPTGVGPGVFYGDQQTGPLPTPPGSESSYENQYLQLHSAASGGHPGGQKTSADAFTNLVSTYGGYHSSIDYHNAMTPPSSVSPRDSNQPGKAAPVLASNGGYDYAPDPLRGQYATSSGDVVPATLPLKPQASYTATMHPSGSTTTEGGVTYSNLDQPQYFAPHSSFHLYHKGSPASGWYSTPS

Target ORF information:

RefSeq Version	NM_001274281.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster trachealess (trh), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274281.1

ORF Insert Sequence:

ATGTTGCCGT ACCAGGCAGC CGTGGCCATG GACTACGCCG GATACCAGAG ACAGCCCACG 
CCCGGACATC CCGGAAGCCA CATGGCCACC ATGGGATCTC TGGGCATGCC CGCGGTGCCC 
TTCACCCACA GCTGGATGGT GCCCACGCAG GACCTTTGCG CCATGCCACC CTACAACAAA 
ATGACCGGAC ACCAGCAGCC GCCAGGTGCC GGAATGCACG CCCAGCAACA GCCCCTCGAG 
CCCGGCATCC TGGAGCTGCG CAAGGAGAAG TCGAGGGACG CGGCGAGATC GCGGCGCGGA 
AAGGAGAACT ACGAGTTCTA CGAGCTAGCC AAGATGCTCC CTCTGCCGGC AGCCATCACC 
AGCCAGCTGG ACAAGGCCTC GATCATAAGA CTGACCATCA GCTACCTGAA GCTGAGGGAT 
TTCTCCGGAC ACGGCGATCC GCCATGGACT CGGGAAGCCT CTAGCAGCAG TAAGCTAAAA 
AGTGCCGCCA TTCGTCGCAG CCCCGCTGTT GATTTGTTCG AGCAACATCA GGGCACCCAC 
ATATTACAGT CGCTGGATGG TTTCGCCCTA GCTGTGGCGG CAGACGGACG CTTCCTGTAT 
ATTTCCGAGA CGGTGTCCAT CTATTTGGGC CTGTCGCAGG TGGAGATGAC GGGTAGCAGC 
ATCTTCGACT ATATCCACCA GGCGGATCAC TCGGAGATCG CCGACCAGCT GGGCCTGAGC 
CTAACAAGCG GCGGCGGCGG TGGCGGTGGA AGCTCGAGCA GCGGCGGAGG AGGAGGCGGA 
GCGGGAGGTG GTATGGCCTC ACCCACTTCC GGAGCCTCCG ACGATGGAAG CGGCACACAC 
GTTGCGGCCT CCATGACCCA AGCTTCGACC AGCGGCTACA AGGGATACGA TCGAAGCTTC 
TGTGTCCGTA TGAAGTCCAC GCTGACCAAG CGCGGCTGTC ACTTCAAATC CTCAGGCTAT 
CGGGTGGTTC TGCTGTTGTG CAAGCTGCGT CCCCAGTACA CATTTTCCCA CAGTCGCAAA 
TCGCAGCCTC CACTCCTCGG AATGGTTGCC CTGGCCATCG CACTGCCTCC GCCATCGGTG 
CACGAGATCC GGCTGGAGTG CGACATGTTC GTCACCAGGG TGAACTTTGA CCTGCGAGTA 
GCCCACTGCG AGCCAAGGGT ATCCGATCTG CTGGACTATT CCCCGGAGGA CCTGGTTAAC 
AAGAGCTTGT ACTCACTGTG TCACGCCGAG GATGCCAATC GCCTGCGCAA GAGCCACTCA 
GACCTGATCG AGAAGGGTCA GGTGTTGACC GGATACTACC GACTAATGAA CAAGAGTGGA 
GGCTACACTT GGCTCCAGAC CTGTGCCACT GTCGTCTGCA GCACAAAGAA CGCCGATGAG 
CAGAACATCA TCTGCGTGAA CTATGTGATC AGCAACCGGG AGAACGAGAA CATGATACTG 
GATTGCTGCC AACTGGAACC CAGTCCGGAT AGCATTAAGC ACGAGGAGGG ACTGGGTAAC 
GACAAAAGCA GCGGGTCGCC AGGAGGAGAT GCCAGTGGCG AAGGCAACTC TCACCTGAGT 
GCCGGAGACA TGAAGCTTAA CTCACCGAAA ACCGATTCAG AGGGCCATTC CCACCGCGGC 
AGGGGTCGCA GTGCAGCCGC CTCGCATGGA AGTTCAATGA ACAGCCTCAC CATGATCAAG 
GATAGCCCCA CTCCGCTGGG TGTGGAGATC GATTCGGGAG TGCTGCCCAC CACAGTGGCC 
ACTCCAGTGC CGGCAGCCAC TCCACCAGTG CAGTCCACCA AGCGAAAGCG AAAAACCAAA 
GCGTCGCAGC ACGCGGAGGA CCAGGGTCAA GAGCAGGTAA TCTCCGAGCA GCCTCTGCCC 
AAACTACCGA CAATGGAGCA ACGCGATCAG CAGCCCCGCA GCCGCTTACC CTCGATTGTG 
GATGAGCAGC CCTCGTCCGC GGCCGACTCC GCGGTCAAGG ATCTGGAGCA GGCCATGTCC 
AAGCATCTGC CCTCGCCAGC GGCGGTGGTG TCCGTGGCCC CGCCCAATAC GGATTTCAGT 
GCCGACTCTC TGCTTAAGCA GCAGCAACAA CAACAGCAGC TGGATCCCAA CGAGAAAAGC 
AGCACCATTC AATGGATAGG CACTCCCTAC CAACAGCCAC CGGCGCCCAT GCCAGCTACC 
GCCCTTCTCC GGCAGTTATA CGCCAACCGG GAGAGCGTGA TCCGGGCGAC GGCTAGGCAA 
ACACCAACTG GAGTGGGCCC CGGAGTTTTC TACGGCGATC AGCAAACAGG TCCGCTGCCC 
ACTCCGCCGG GCAGTGAGTC GTCCTACGAG AACCAATACC TGCAGCTGCA CTCTGCCGCT 
TCCGGTGGAC ATCCTGGCGG CCAGAAGACC TCCGCTGATG CCTTCACCAA TCTGGTGTCA 
ACCTACGGCG GCTATCACAG CTCCATTGAC TATCACAACG CCATGACCCC GCCCAGTTCA 
GTGTCTCCCA GGGACTCGAA TCAACCGGGT AAGGCGGCTC CAGTCCTGGC CTCCAACGGG 
GGATACGACT ATGCCCCCGA TCCACTCCGA GGGCAGTACG CCACTTCATC CGGAGACGTG 
GTGCCCGCCA CGCTGCCTCT GAAGCCCCAG GCATCCTATA CAGCAACAAT GCATCCTTCG 
GGCAGCACAA CCACCGAGGG TGGAGTTACC TACAGTAATC TCGACCAGCC GCAGTACTTC 
GCTCCGCATT CGAGTTTCCA CCTTTACCAC AAGGGCAGCC CGGCCAGCGG CTGGTACTCC 
ACGCCCTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trh ( NM_001274279.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261208.1 Drosophila melanogaster trachealess (trh), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa17071
Clone ID Related Accession (Same CDS sequence)	NM_001274279.1
Accession Version	NM_001274279.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2859bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	trachealess, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCCGT ACCAGGCAGC CGTGGCCATG GACTACGCCG GATACCAGAG ACAGCCCACG 
CCCGGACATC CCGGAAGCCA CATGGCCACC ATGGGATCTC TGGGCATGCC CGCGGTGCCC 
TTCACCCACA GCTGGATGGT GCCCACGCAG GACCTTTGCG CCATGCCACC CTACAACAAA 
ATGACCGGAC ACCAGCAGCC GCCAGGTGCC GGAATGCACG CCCAGCAACA GCCCCTCGAG 
CCCGGCATCC TGGAGCTGCG CAAGGAGAAG TCGAGGGACG CGGCGAGATC GCGGCGCGGA 
AAGGAGAACT ACGAGTTCTA CGAGCTAGCC AAGATGCTCC CTCTGCCGGC AGCCATCACC 
AGCCAGCTGG ACAAGGCCTC GATCATAAGA CTGACCATCA GCTACCTGAA GCTGAGGGAT 
TTCTCCGGAC ACGGCGATCC GCCATGGACT CGGGAAGCCT CTAGCAGCAG TAAGCTAAAA 
AGTGCCGCCA TTCGTCGCAG CCCCGCTGTT GATTTGTTCG AGCAACATCA GGGCACCCAC 
ATATTACAGT CGCTGGATGG TTTCGCCCTA GCTGTGGCGG CAGACGGACG CTTCCTGTAT 
ATTTCCGAGA CGGTGTCCAT CTATTTGGGC CTGTCGCAGG TGGAGATGAC GGGTAGCAGC 
ATCTTCGACT ATATCCACCA GGCGGATCAC TCGGAGATCG CCGACCAGCT GGGCCTGAGC 
CTAACAAGCG GCGGCGGCGG TGGCGGTGGA AGCTCGAGCA GCGGCGGAGG AGGAGGCGGA 
GCGGGAGGTG GTATGGCCTC ACCCACTTCC GGAGCCTCCG ACGATGGAAG CGGCACACAC 
GTTGCGGCCT CCATGACCCA AGCTTCGACC AGCGGCTACA AGGGATACGA TCGAAGCTTC 
TGTGTCCGTA TGAAGTCCAC GCTGACCAAG CGCGGCTGTC ACTTCAAATC CTCAGGCTAT 
CGGGCCAGCG ATGCAACGAG CAATTGCAAC AACGGTAACA ATGCTAGTAA CAATGCTAAA 
AACGTTAAGA ATCCGGGCTC AAACTATTCG GTGGTTCTGC TGTTGTGCAA GCTGCGTCCC 
CAGTACACAT TTTCCCACAG TCGCAAATCG CAGCCTCCAC TCCTCGGAAT GGTTGCCCTG 
GCCATCGCAC TGCCTCCGCC ATCGGTGCAC GAGATCCGGC TGGAGTGCGA CATGTTCGTC 
ACCAGGGTGA ACTTTGACCT GCGAGTAGCC CACTGCGAGC CAAGGGTATC CGATCTGCTG 
GACTATTCCC CGGAGGACCT GGTTAACAAG AGCTTGTACT CACTGTGTCA CGCCGAGGAT 
GCCAATCGCC TGCGCAAGAG CCACTCAGAC CTGATCGAGA AGGGTCAGGT GTTGACCGGA 
TACTACCGAC TAATGAACAA GAGTGGAGGC TACACTTGGC TCCAGACCTG TGCCACTGTC 
GTCTGCAGCA CAAAGAACGC CGATGAGCAG AACATCATCT GCGTGAACTA TGTGATCAGC 
AACCGGGAGA ACGAGAACAT GATACTGGAT TGCTGCCAAC TGGAACCCAG TCCGGATAGC 
ATTAAGCACG AGGAGGGACT GGGTAACGAC AAAAGCAGCG GGTCGCCAGG AGGAGATGCC 
AGTGGCGAAG GCAACTCTCA CCTGAGTGCC GGAGACATGA AGCTTAACTC ACCGAAAACC 
GATTCAGAGG GCCATTCCCA CCGCGGCAGG GGTCGCAGTG CAGCCGCCTC GCATGGAAGT 
TCAATGAACA GCCTCACCAT GATCAAGGAT AGCCCCACTC CGCTGGGTGT GGAGATCGAT 
TCGGGAGTGC TGCCCACCAC AGTGGCCACT CCAGTGCCGG CAGCCACTCC ACCAGTGCAG 
TCCACCAAGC GAAAGCGAAA AACCAAAGCG TCGCAGCACG CGGAGGACCA GGGTCAAGAG 
CAGGTAATCT CCGAGCAGCC TCTGCCCAAA CTACCGACAA TGGAGCAACG CGATCAGCAG 
CCCCGCAGCC GCTTACCCTC GATTGTGGAT GAGCAGCCCT CGTCCGCGGC CGACTCCGCG 
GTCAAGGATC TGGAGCAGGC CATGTCCAAG CATCTGCCCT CGCCAGCGGC GGTGGTGTCC 
GTGGCCCCGC CCAATACGGA TTTCAGTGCC GACTCTCTGC TTAAGCAGCA GCAACAACAA 
CAGCAGCTGG ATCCCAACGA GAAAAGCAGC ACCATTCAAT GGATAGGCAC TCCCTACCAA 
CAGCCACCGG CGCCCATGCC AGCTACCGCC CTTCTCCGGC AGTTATACGC CAACCGGGAG 
AGCGTGATCC GGGCGACGGC TAGGCAAACA CCAACTGGAG TGGGCCCCGG AGTTTTCTAC 
GGCGATCAGC AAACAGGTCC GCTGCCCACT CCGCCGGGCA GTGAGTCGTC CTACGAGAAC 
CAATACCTGC AGCTGCACTC TGCCGCTTCC GGTGGACATC CTGGCGGCCA GAAGACCTCC 
GCTGATGCCT TCACCAATCT GGTGTCAACC TACGGCGGCT ATCACAGCTC CATTGACTAT 
CACAACGCCA TGACCCCGCC CAGTTCAGTG TCTCCCAGGG ACTCGAATCA ACCGGGTAAG 
GCGGCTCCAG TCCTGGCCTC CAACGGGGGA TACGACTATG CCCCCGATCC ACTCCGAGGG 
CAGTACGCCA CTTCATCCGG AGACGTGGTG CCCGCCACGC TGCCTCTGAA GCCCCAGGCA 
TCCTATACAG CAACAATGCA TCCTTCGGGC AGCACAACCA CCGAGGGTGG AGTTACCTAC 
AGTAATCTCG ACCAGCCGCA GTACTTCGCT CCGCATTCGA GTTTCCACCT TTACCACAAG 
GGCAGCCCGG CCAGCGGCTG GTACTCCACG CCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261208.1
CDS	654..3512
Translation	MLPYQAAVAMDYAGYQRQPTPGHPGSHMATMGSLGMPAVPFTHSWMVPTQDLCAMPPYNKMTGHQQPPGAGMHAQQQPLEPGILELRKEKSRDAARSRRGKENYEFYELAKMLPLPAAITSQLDKASIIRLTISYLKLRDFSGHGDPPWTREASSSSKLKSAAIRRSPAVDLFEQHQGTHILQSLDGFALAVAADGRFLYISETVSIYLGLSQVEMTGSSIFDYIHQADHSEIADQLGLSLTSGGGGGGGSSSSGGGGGGAGGGMASPTSGASDDGSGTHVAASMTQASTSGYKGYDRSFCVRMKSTLTKRGCHFKSSGYRASDATSNCNNGNNASNNAKNVKNPGSNYSVVLLLCKLRPQYTFSHSRKSQPPLLGMVALAIALPPPSVHEIRLECDMFVTRVNFDLRVAHCEPRVSDLLDYSPEDLVNKSLYSLCHAEDANRLRKSHSDLIEKGQVLTGYYRLMNKSGGYTWLQTCATVVCSTKNADEQNIICVNYVISNRENENMILDCCQLEPSPDSIKHEEGLGNDKSSGSPGGDASGEGNSHLSAGDMKLNSPKTDSEGHSHRGRGRSAAASHGSSMNSLTMIKDSPTPLGVEIDSGVLPTTVATPVPAATPPVQSTKRKRKTKASQHAEDQGQEQVISEQPLPKLPTMEQRDQQPRSRLPSIVDEQPSSAADSAVKDLEQAMSKHLPSPAAVVSVAPPNTDFSADSLLKQQQQQQQLDPNEKSSTIQWIGTPYQQPPAPMPATALLRQLYANRESVIRATARQTPTGVGPGVFYGDQQTGPLPTPPGSESSYENQYLQLHSAASGGHPGGQKTSADAFTNLVSTYGGYHSSIDYHNAMTPPSSVSPRDSNQPGKAAPVLASNGGYDYAPDPLRGQYATSSGDVVPATLPLKPQASYTATMHPSGSTTTEGGVTYSNLDQPQYFAPHSSFHLYHKGSPASGWYSTPS

Target ORF information:

RefSeq Version	NM_001274279.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster trachealess (trh), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274279.1

ORF Insert Sequence:

ATGTTGCCGT ACCAGGCAGC CGTGGCCATG GACTACGCCG GATACCAGAG ACAGCCCACG 
CCCGGACATC CCGGAAGCCA CATGGCCACC ATGGGATCTC TGGGCATGCC CGCGGTGCCC 
TTCACCCACA GCTGGATGGT GCCCACGCAG GACCTTTGCG CCATGCCACC CTACAACAAA 
ATGACCGGAC ACCAGCAGCC GCCAGGTGCC GGAATGCACG CCCAGCAACA GCCCCTCGAG 
CCCGGCATCC TGGAGCTGCG CAAGGAGAAG TCGAGGGACG CGGCGAGATC GCGGCGCGGA 
AAGGAGAACT ACGAGTTCTA CGAGCTAGCC AAGATGCTCC CTCTGCCGGC AGCCATCACC 
AGCCAGCTGG ACAAGGCCTC GATCATAAGA CTGACCATCA GCTACCTGAA GCTGAGGGAT 
TTCTCCGGAC ACGGCGATCC GCCATGGACT CGGGAAGCCT CTAGCAGCAG TAAGCTAAAA 
AGTGCCGCCA TTCGTCGCAG CCCCGCTGTT GATTTGTTCG AGCAACATCA GGGCACCCAC 
ATATTACAGT CGCTGGATGG TTTCGCCCTA GCTGTGGCGG CAGACGGACG CTTCCTGTAT 
ATTTCCGAGA CGGTGTCCAT CTATTTGGGC CTGTCGCAGG TGGAGATGAC GGGTAGCAGC 
ATCTTCGACT ATATCCACCA GGCGGATCAC TCGGAGATCG CCGACCAGCT GGGCCTGAGC 
CTAACAAGCG GCGGCGGCGG TGGCGGTGGA AGCTCGAGCA GCGGCGGAGG AGGAGGCGGA 
GCGGGAGGTG GTATGGCCTC ACCCACTTCC GGAGCCTCCG ACGATGGAAG CGGCACACAC 
GTTGCGGCCT CCATGACCCA AGCTTCGACC AGCGGCTACA AGGGATACGA TCGAAGCTTC 
TGTGTCCGTA TGAAGTCCAC GCTGACCAAG CGCGGCTGTC ACTTCAAATC CTCAGGCTAT 
CGGGCCAGCG ATGCAACGAG CAATTGCAAC AACGGTAACA ATGCTAGTAA CAATGCTAAA 
AACGTTAAGA ATCCGGGCTC AAACTATTCG GTGGTTCTGC TGTTGTGCAA GCTGCGTCCC 
CAGTACACAT TTTCCCACAG TCGCAAATCG CAGCCTCCAC TCCTCGGAAT GGTTGCCCTG 
GCCATCGCAC TGCCTCCGCC ATCGGTGCAC GAGATCCGGC TGGAGTGCGA CATGTTCGTC 
ACCAGGGTGA ACTTTGACCT GCGAGTAGCC CACTGCGAGC CAAGGGTATC CGATCTGCTG 
GACTATTCCC CGGAGGACCT GGTTAACAAG AGCTTGTACT CACTGTGTCA CGCCGAGGAT 
GCCAATCGCC TGCGCAAGAG CCACTCAGAC CTGATCGAGA AGGGTCAGGT GTTGACCGGA 
TACTACCGAC TAATGAACAA GAGTGGAGGC TACACTTGGC TCCAGACCTG TGCCACTGTC 
GTCTGCAGCA CAAAGAACGC CGATGAGCAG AACATCATCT GCGTGAACTA TGTGATCAGC 
AACCGGGAGA ACGAGAACAT GATACTGGAT TGCTGCCAAC TGGAACCCAG TCCGGATAGC 
ATTAAGCACG AGGAGGGACT GGGTAACGAC AAAAGCAGCG GGTCGCCAGG AGGAGATGCC 
AGTGGCGAAG GCAACTCTCA CCTGAGTGCC GGAGACATGA AGCTTAACTC ACCGAAAACC 
GATTCAGAGG GCCATTCCCA CCGCGGCAGG GGTCGCAGTG CAGCCGCCTC GCATGGAAGT 
TCAATGAACA GCCTCACCAT GATCAAGGAT AGCCCCACTC CGCTGGGTGT GGAGATCGAT 
TCGGGAGTGC TGCCCACCAC AGTGGCCACT CCAGTGCCGG CAGCCACTCC ACCAGTGCAG 
TCCACCAAGC GAAAGCGAAA AACCAAAGCG TCGCAGCACG CGGAGGACCA GGGTCAAGAG 
CAGGTAATCT CCGAGCAGCC TCTGCCCAAA CTACCGACAA TGGAGCAACG CGATCAGCAG 
CCCCGCAGCC GCTTACCCTC GATTGTGGAT GAGCAGCCCT CGTCCGCGGC CGACTCCGCG 
GTCAAGGATC TGGAGCAGGC CATGTCCAAG CATCTGCCCT CGCCAGCGGC GGTGGTGTCC 
GTGGCCCCGC CCAATACGGA TTTCAGTGCC GACTCTCTGC TTAAGCAGCA GCAACAACAA 
CAGCAGCTGG ATCCCAACGA GAAAAGCAGC ACCATTCAAT GGATAGGCAC TCCCTACCAA 
CAGCCACCGG CGCCCATGCC AGCTACCGCC CTTCTCCGGC AGTTATACGC CAACCGGGAG 
AGCGTGATCC GGGCGACGGC TAGGCAAACA CCAACTGGAG TGGGCCCCGG AGTTTTCTAC 
GGCGATCAGC AAACAGGTCC GCTGCCCACT CCGCCGGGCA GTGAGTCGTC CTACGAGAAC 
CAATACCTGC AGCTGCACTC TGCCGCTTCC GGTGGACATC CTGGCGGCCA GAAGACCTCC 
GCTGATGCCT TCACCAATCT GGTGTCAACC TACGGCGGCT ATCACAGCTC CATTGACTAT 
CACAACGCCA TGACCCCGCC CAGTTCAGTG TCTCCCAGGG ACTCGAATCA ACCGGGTAAG 
GCGGCTCCAG TCCTGGCCTC CAACGGGGGA TACGACTATG CCCCCGATCC ACTCCGAGGG 
CAGTACGCCA CTTCATCCGG AGACGTGGTG CCCGCCACGC TGCCTCTGAA GCCCCAGGCA 
TCCTATACAG CAACAATGCA TCCTTCGGGC AGCACAACCA CCGAGGGTGG AGTTACCTAC 
AGTAATCTCG ACCAGCCGCA GTACTTCGCT CCGCATTCGA GTTTCCACCT TTACCACAAG 
GGCAGCCCGG CCAGCGGCTG GTACTCCACG CCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trh ( NM_001103991.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097461.2 Drosophila melanogaster trachealess (trh), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa20352
Clone ID Related Accession (Same CDS sequence)	NM_001103991.2
Accession Version	NM_001103991.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2901bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	trachealess, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 31, 2011 this sequence version replaced NM_001103991.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCATC ACGGCAGTGG GTTCGTGGCT TCGCCATGGG CCGCCGTTCT GGGACACCAC 
TCGATGGCCT CCGATGCCGG GTTCGCCGCT GCGGCTGCTG CGGCGCATGT CCAGAACCAC 
TCGATGCACC ATCCTATACA CTCGCATCAC CACCACCACT CGCACTCGCA TCCACACCCA 
CATCCGCACT CGCATCCGCA CCACCATCCG CACCTGGGAA CCGCCGGAGG GATGCCGATG 
GATCTGCATG TGCCGCAGGG ATTCCCCTAC TACAGATACC GAGAGGACGC ACTGTGCTGG 
GGCGACAGAA AGTCCATGGA GGAGATCGGA GCGGCCCAGT CCTCGGTGAA TGCGCGCATC 
CTGGAGCTGC GCAAGGAGAA GTCGAGGGAC GCGGCGAGAT CGCGGCGCGG AAAGGAGAAC 
TACGAGTTCT ACGAGCTAGC CAAGATGCTC CCTCTGCCGG CAGCCATCAC CAGCCAGCTG 
GACAAGGCCT CGATCATAAG ACTGACCATC AGCTACCTGA AGCTGAGGGA TTTCTCCGGA 
CACGGCGATC CGCCATGGAC TCGGGAAGCC TCTAGCAGCA GTAAGCTAAA AAGTGCCGCC 
ATTCGTCGCA GCCCCGCTGT TGATTTGTTC GAGCAACATC AGGGCACCCA CATATTACAG 
TCGCTGGATG GTTTCGCCCT AGCTGTGGCG GCAGACGGAC GCTTCCTGTA TATTTCCGAG 
ACGGTGTCCA TCTATTTGGG CCTGTCGCAG GTGGAGATGA CGGGTAGCAG CATCTTCGAC 
TATATCCACC AGGCGGATCA CTCGGAGATC GCCGACCAGC TGGGCCTGAG CCTAACAAGC 
GGCGGCGGCG GTGGCGGTGG AAGCTCGAGC AGCGGCGGAG GAGGAGGCGG AGCGGGAGGT 
GGTATGGCCT CACCCACTTC CGGAGCCTCC GACGATGGAA GCGGCACACA CGGTACGAAC 
AATCCCGACG TTGCGGCCTC CATGACCCAA GCTTCGACCA GCGGCTACAA GGGATACGAT 
CGAAGCTTCT GTGTCCGTAT GAAGTCCACG CTGACCAAGC GCGGCTGTCA CTTCAAATCC 
TCAGGCTATC GGGTGGTTCT GCTGTTGTGC AAGCTGCGTC CCCAGTACAC ATTTTCCCAC 
AGTCGCAAAT CGCAGCCTCC ACTCCTCGGA ATGGTTGCCC TGGCCATCGC ACTGCCTCCG 
CCATCGGTGC ACGAGATCCG GCTGGAGTGC GACATGTTCG TCACCAGGGT GAACTTTGAC 
CTGCGAGTAG CCCACTGCGA GCCAAGGGTA TCCGATCTGC TGGACTATTC CCCGGAGGAC 
CTGGTTAACA AGAGCTTGTA CTCACTGTGT CACGCCGAGG ATGCCAATCG CCTGCGCAAG 
AGCCACTCAG ACCTGATCGA GAAGGGTCAG GTGTTGACCG GATACTACCG ACTAATGAAC 
AAGAGTGGAG GCTACACTTG GCTCCAGACC TGTGCCACTG TCGTCTGCAG CACAAAGAAC 
GCCGATGAGC AGAACATCAT CTGCGTGAAC TATGTGATCA GCAACCGGGA GAACGAGAAC 
ATGATACTGG ATTGCTGCCA ACTGGAACCC AGTCCGGATA GCATTAAGCA CGAGGAGGGA 
CTGGGTAACG ACAAAAGCAG CGGGTCGCCA GGAGGAGATG CCAGTGGCGA AGGCAACTCT 
CACCTGAGTG CCGGAGACAT GAAGCTTAAC TCACCGAAAA CCGATTCAGA GGGCCATTCC 
CACCGCGGCA GGGGTCGCAG TGCAGCCGCC TCGCATGGAA GTTCAATGAA CAGCCTCACC 
ATGATCAAGG ATAGCCCCAC TCCGCTGGGT GTGGAGATCG ATTCGGGAGT GCTGCCCACC 
ACAGTGGCCA CTCCAGTGCC GGCAGCCACT CCACCAGTGC AGTCCACCAA GCGAAAGCGA 
AAAACCAAAG CGTCGCAGCA CGCGGAGGAC CAGGGTCAAG AGCAGGTAAT CTCCGAGCAG 
CCTCTGCCCA AACTACCGAC AATGGAGCAA CGCGATCAGC AGCCCCGCAG CCGCTTACCC 
TCGATTGTGG ATGAGCAGCC CTCGTCCGCG GCCGACTCCG CGGTCAAGGA TCTGGAGCAG 
GCCATGTCCA AGCATCTGCC CTCGCCAGCG GCGGTGGTGT CCGTGGCCCC GCCCAATACG 
GATTTCAGTG CCGACTCTCT GCTTAAGCAG CAGCAACAAC AACAGCAGCT GGATCCCAAC 
GAGAAAAGCA GCACCATTCA ATGGATAGGC ACTCCCTACC AACAGCCACC GGCGCCCATG 
CCAGCTACCG CCCTTCTCCG GCAGTTATAC GCCAACCGGG AGAGCGTGAT CCGGGCGACG 
GCTAGGCAAA CACCAACTGG AGTGGGCCCC GGAGTTTTCT ACGGCGATCA GCAAACAGGT 
CCGCTGCCCA CTCCGCCGGG CAGTGAGTCG TCCTACGAGA ACCAATACCT GCAGCTGCAC 
TCTGCCGCTT CCGGTGGACA TCCTGGCGGC CAGAAGACCT CCGCTGATGC CTTCACCAAT 
CTGGTGTCAA CCTACGGCGG CTATCACAGC TCCATTGACT ATCACAACGC CATGACCCCG 
CCCAGTTCAG TGTCTCCCAG GGACTCGAAT CAACCGGGTA AGGCGGCTCC AGTCCTGGCC 
TCCAACGGGG GATACGACTA TGCCCCCGAT CCACTCCGAG GGCAGTACGC CACTTCATCC 
GGAGACGTGG TGCCCGCCAC GCTGCCTCTG AAGCCCCAGG CATCCTATAC AGCAACAATG 
CATCCTTCGG GCAGCACAAC CACCGAGGGT GGAGTTACCT ACAGTAATCT CGACCAGCCG 
CAGTACTTCG CTCCGCATTC GAGTTTCCAC CTTTACCACA AGGGCAGCCC GGCCAGCGGC 
TGGTACTCCA CGCCCTCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097461.2
CDS	559..3459
Translation	MEHHGSGFVASPWAAVLGHHSMASDAGFAAAAAAAHVQNHSMHHPIHSHHHHHSHSHPHPHPHSHPHHHPHLGTAGGMPMDLHVPQGFPYYRYREDALCWGDRKSMEEIGAAQSSVNARILELRKEKSRDAARSRRGKENYEFYELAKMLPLPAAITSQLDKASIIRLTISYLKLRDFSGHGDPPWTREASSSSKLKSAAIRRSPAVDLFEQHQGTHILQSLDGFALAVAADGRFLYISETVSIYLGLSQVEMTGSSIFDYIHQADHSEIADQLGLSLTSGGGGGGGSSSSGGGGGGAGGGMASPTSGASDDGSGTHGTNNPDVAASMTQASTSGYKGYDRSFCVRMKSTLTKRGCHFKSSGYRVVLLLCKLRPQYTFSHSRKSQPPLLGMVALAIALPPPSVHEIRLECDMFVTRVNFDLRVAHCEPRVSDLLDYSPEDLVNKSLYSLCHAEDANRLRKSHSDLIEKGQVLTGYYRLMNKSGGYTWLQTCATVVCSTKNADEQNIICVNYVISNRENENMILDCCQLEPSPDSIKHEEGLGNDKSSGSPGGDASGEGNSHLSAGDMKLNSPKTDSEGHSHRGRGRSAAASHGSSMNSLTMIKDSPTPLGVEIDSGVLPTTVATPVPAATPPVQSTKRKRKTKASQHAEDQGQEQVISEQPLPKLPTMEQRDQQPRSRLPSIVDEQPSSAADSAVKDLEQAMSKHLPSPAAVVSVAPPNTDFSADSLLKQQQQQQQLDPNEKSSTIQWIGTPYQQPPAPMPATALLRQLYANRESVIRATARQTPTGVGPGVFYGDQQTGPLPTPPGSESSYENQYLQLHSAASGGHPGGQKTSADAFTNLVSTYGGYHSSIDYHNAMTPPSSVSPRDSNQPGKAAPVLASNGGYDYAPDPLRGQYATSSGDVVPATLPLKPQASYTATMHPSGSTTTEGGVTYSNLDQPQYFAPHSSFHLYHKGSPASGWYSTPS

Target ORF information:

RefSeq Version	NM_001103991.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster trachealess (trh), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103991.2

ORF Insert Sequence:

ATGGAGCATC ACGGCAGTGG GTTCGTGGCT TCGCCATGGG CCGCCGTTCT GGGACACCAC 
TCGATGGCCT CCGATGCCGG GTTCGCCGCT GCGGCTGCTG CGGCGCATGT CCAGAACCAC 
TCGATGCACC ATCCTATACA CTCGCATCAC CACCACCACT CGCACTCGCA TCCACACCCA 
CATCCGCACT CGCATCCGCA CCACCATCCG CACCTGGGAA CCGCCGGAGG GATGCCGATG 
GATCTGCATG TGCCGCAGGG ATTCCCCTAC TACAGATACC GAGAGGACGC ACTGTGCTGG 
GGCGACAGAA AGTCCATGGA GGAGATCGGA GCGGCCCAGT CCTCGGTGAA TGCGCGCATC 
CTGGAGCTGC GCAAGGAGAA GTCGAGGGAC GCGGCGAGAT CGCGGCGCGG AAAGGAGAAC 
TACGAGTTCT ACGAGCTAGC CAAGATGCTC CCTCTGCCGG CAGCCATCAC CAGCCAGCTG 
GACAAGGCCT CGATCATAAG ACTGACCATC AGCTACCTGA AGCTGAGGGA TTTCTCCGGA 
CACGGCGATC CGCCATGGAC TCGGGAAGCC TCTAGCAGCA GTAAGCTAAA AAGTGCCGCC 
ATTCGTCGCA GCCCCGCTGT TGATTTGTTC GAGCAACATC AGGGCACCCA CATATTACAG 
TCGCTGGATG GTTTCGCCCT AGCTGTGGCG GCAGACGGAC GCTTCCTGTA TATTTCCGAG 
ACGGTGTCCA TCTATTTGGG CCTGTCGCAG GTGGAGATGA CGGGTAGCAG CATCTTCGAC 
TATATCCACC AGGCGGATCA CTCGGAGATC GCCGACCAGC TGGGCCTGAG CCTAACAAGC 
GGCGGCGGCG GTGGCGGTGG AAGCTCGAGC AGCGGCGGAG GAGGAGGCGG AGCGGGAGGT 
GGTATGGCCT CACCCACTTC CGGAGCCTCC GACGATGGAA GCGGCACACA CGGTACGAAC 
AATCCCGACG TTGCGGCCTC CATGACCCAA GCTTCGACCA GCGGCTACAA GGGATACGAT 
CGAAGCTTCT GTGTCCGTAT GAAGTCCACG CTGACCAAGC GCGGCTGTCA CTTCAAATCC 
TCAGGCTATC GGGTGGTTCT GCTGTTGTGC AAGCTGCGTC CCCAGTACAC ATTTTCCCAC 
AGTCGCAAAT CGCAGCCTCC ACTCCTCGGA ATGGTTGCCC TGGCCATCGC ACTGCCTCCG 
CCATCGGTGC ACGAGATCCG GCTGGAGTGC GACATGTTCG TCACCAGGGT GAACTTTGAC 
CTGCGAGTAG CCCACTGCGA GCCAAGGGTA TCCGATCTGC TGGACTATTC CCCGGAGGAC 
CTGGTTAACA AGAGCTTGTA CTCACTGTGT CACGCCGAGG ATGCCAATCG CCTGCGCAAG 
AGCCACTCAG ACCTGATCGA GAAGGGTCAG GTGTTGACCG GATACTACCG ACTAATGAAC 
AAGAGTGGAG GCTACACTTG GCTCCAGACC TGTGCCACTG TCGTCTGCAG CACAAAGAAC 
GCCGATGAGC AGAACATCAT CTGCGTGAAC TATGTGATCA GCAACCGGGA GAACGAGAAC 
ATGATACTGG ATTGCTGCCA ACTGGAACCC AGTCCGGATA GCATTAAGCA CGAGGAGGGA 
CTGGGTAACG ACAAAAGCAG CGGGTCGCCA GGAGGAGATG CCAGTGGCGA AGGCAACTCT 
CACCTGAGTG CCGGAGACAT GAAGCTTAAC TCACCGAAAA CCGATTCAGA GGGCCATTCC 
CACCGCGGCA GGGGTCGCAG TGCAGCCGCC TCGCATGGAA GTTCAATGAA CAGCCTCACC 
ATGATCAAGG ATAGCCCCAC TCCGCTGGGT GTGGAGATCG ATTCGGGAGT GCTGCCCACC 
ACAGTGGCCA CTCCAGTGCC GGCAGCCACT CCACCAGTGC AGTCCACCAA GCGAAAGCGA 
AAAACCAAAG CGTCGCAGCA CGCGGAGGAC CAGGGTCAAG AGCAGGTAAT CTCCGAGCAG 
CCTCTGCCCA AACTACCGAC AATGGAGCAA CGCGATCAGC AGCCCCGCAG CCGCTTACCC 
TCGATTGTGG ATGAGCAGCC CTCGTCCGCG GCCGACTCCG CGGTCAAGGA TCTGGAGCAG 
GCCATGTCCA AGCATCTGCC CTCGCCAGCG GCGGTGGTGT CCGTGGCCCC GCCCAATACG 
GATTTCAGTG CCGACTCTCT GCTTAAGCAG CAGCAACAAC AACAGCAGCT GGATCCCAAC 
GAGAAAAGCA GCACCATTCA ATGGATAGGC ACTCCCTACC AACAGCCACC GGCGCCCATG 
CCAGCTACCG CCCTTCTCCG GCAGTTATAC GCCAACCGGG AGAGCGTGAT CCGGGCGACG 
GCTAGGCAAA CACCAACTGG AGTGGGCCCC GGAGTTTTCT ACGGCGATCA GCAAACAGGT 
CCGCTGCCCA CTCCGCCGGG CAGTGAGTCG TCCTACGAGA ACCAATACCT GCAGCTGCAC 
TCTGCCGCTT CCGGTGGACA TCCTGGCGGC CAGAAGACCT CCGCTGATGC CTTCACCAAT 
CTGGTGTCAA CCTACGGCGG CTATCACAGC TCCATTGACT ATCACAACGC CATGACCCCG 
CCCAGTTCAG TGTCTCCCAG GGACTCGAAT CAACCGGGTA AGGCGGCTCC AGTCCTGGCC 
TCCAACGGGG GATACGACTA TGCCCCCGAT CCACTCCGAG GGCAGTACGC CACTTCATCC 
GGAGACGTGG TGCCCGCCAC GCTGCCTCTG AAGCCCCAGG CATCCTATAC AGCAACAATG 
CATCCTTCGG GCAGCACAAC CACCGAGGGT GGAGTTACCT ACAGTAATCT CGACCAGCCG 
CAGTACTTCG CTCCGCATTC GAGTTTCCAC CTTTACCACA AGGGCAGCCC GGCCAGCGGC 
TGGTACTCCA CGCCCTCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trh ( NM_001043110.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036575.1 Drosophila melanogaster trachealess (trh), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa20354
Clone ID Related Accession (Same CDS sequence)	NM_001043110.2
Accession Version	NM_001043110.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	trachealess, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Dec 17, 2009 this sequence version replaced NM_001043110.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCCGT ACCAGGCAGC CGTGGCCATG GACTACGCCG GATACCAGAG ACAGCCCACG 
CCCGGACATC CCGGAAGCCA CATGGCCACC ATGGGATCTC TGGGCATGCC CGCGGTGCCC 
TTCACCCACA GCTGGATGGT GCCCACGCAG GACCTTTGCG CCATGCCACC CTACAACAAA 
ATGACCGGAC ACCAGCAGCC GCCAGGTGCC GGAATGCACG CCCAGCAACA GCCCCTCGAG 
CCCGGCATCC TGGAGCTGCG CAAGGAGAAG TCGAGGGACG CGGCGAGATC GCGGCGCGGA 
AAGGAGAACT ACGAGTTCTA CGAGCTAGCC AAGATGCTCC CTCTGCCGGC AGCCATCACC 
AGCCAGCTGG ACAAGGCCTC GATCATAAGA CTGACCATCA GCTACCTGAA GCTGAGGGAT 
TTCTCCGGAC ACGGCGATCC GCCATGGACT CGGGAAGCCT CTAGCAGCAG TAAGCTAAAA 
AGTGCCGCCA TTCGTCGCAG CCCCGCTGTT GATTTGTTCG AGCAACATCA GGGCACCCAC 
ATATTACAGT CGCTGGATGG TTTCGCCCTA GCTGTGGCGG CAGACGGACG CTTCCTGTAT 
ATTTCCGAGA CGGTGTCCAT CTATTTGGGC CTGTCGCAGG TGGAGATGAC GGGTAGCAGC 
ATCTTCGACT ATATCCACCA GGCGGATCAC TCGGAGATCG CCGACCAGCT GGGCCTGAGC 
CTAACAAGCG GCGGCGGCGG TGGCGGTGGA AGCTCGAGCA GCGGCGGAGG AGGAGGCGGA 
GCGGGAGGTG GTATGGCCTC ACCCACTTCC GGAGCCTCCG ACGATGGAAG CGGCACACAC 
GGTACGAACA ATCCCGACGT TGCGGCCTCC ATGACCCAAG CTTCGACCAG CGGCTACAAG 
GGATACGATC GAAGCTTCTG TGTCCGTATG AAGTCCACGC TGACCAAGCG CGGCTGTCAC 
TTCAAATCCT CAGGCTATCG GGTGGTTCTG CTGTTGTGCA AGCTGCGTCC CCAGTACACA 
TTTTCCCACA GTCGCAAATC GCAGCCTCCA CTCCTCGGAA TGGTTGCCCT GGCCATCGCA 
CTGCCTCCGC CATCGGTGCA CGAGATCCGG CTGGAGTGCG ACATGTTCGT CACCAGGGTG 
AACTTTGACC TGCGAGTAGC CCACTGCGAG CCAAGGGTAT CCGATCTGCT GGACTATTCC 
CCGGAGGACC TGGTTAACAA GAGCTTGTAC TCACTGTGTC ACGCCGAGGA TGCCAATCGC 
CTGCGCAAGA GCCACTCAGA CCTGATCGAG AAGGGTCAGG TGTTGACCGG ATACTACCGA 
CTAATGAACA AGAGTGGAGG CTACACTTGG CTCCAGACCT GTGCCACTGT CGTCTGCAGC 
ACAAAGAACG CCGATGAGCA GAACATCATC TGCGTGAACT ATGTGATCAG CAACCGGGAG 
AACGAGAACA TGATACTGGA TTGCTGCCAA CTGGAACCCA GTCCGGATAG CATTAAGCAC 
GAGGAGGGAC TGGGTAACGA CAAAAGCAGC GGGTCGCCAG GAGGAGATGC CAGTGGCGAA 
GGCAACTCTC ACCTGAGTGC CGGAGACATG AAGCTTAACT CACCGAAAAC CGATTCAGAG 
GGCCATTCCC ACCGCGGCAG GGGTCGCAGT GCAGCCGCCT CGCATGGAAG TTCAATGAAC 
AGCCTCACCA TGATCAAGGA TAGCCCCACT CCGCTGGGTG TGGAGATCGA TTCGGGAGTG 
CTGCCCACCA CAGTGGCCAC TCCAGTGCCG GCAGCCACTC CACCAGTGCA GTCCACCAAG 
CGAAAGCGAA AAACCAAAGC GTCGCAGCAC GCGGAGGACC AGGGTCAAGA GCAGGTAATC 
TCCGAGCAGC CTCTGCCCAA ACTACCGACA ATGGAGCAAC GCGATCAGCA GCCCCGCAGC 
CGCTTACCCT CGATTGTGGA TGAGCAGCCC TCGTCCGCGG CCGACTCCGC GGTCAAGGAT 
CTGGAGCAGG CCATGTCCAA GCATCTGCCC TCGCCAGCGG CGGTGGTGTC CGTGGCCCCG 
CCCAATACGG ATTTCAGTGC CGACTCTCTG CTTAAGCAGC AGCAACAACA ACAGCAGCTG 
GATCCCAACG AGAAAAGCAG CACCATTCAA TGGATAGGCA CTCCCTACCA ACAGCCACCG 
GCGCCCATGC CAGCTACCGC CCTTCTCCGG CAGTTATACG CCAACCGGGA GAGCGTGATC 
CGGGCGACGG CTAGGCAAAC ACCAACTGGA GTGGGCCCCG GAGTTTTCTA CGGCGATCAG 
CAAACAGGTC CGCTGCCCAC TCCGCCGGGC AGTGAGTCGT CCTACGAGAA CCAATACCTG 
CAGCTGCACT CTGCCGCTTC CGGTGGACAT CCTGGCGGCC AGAAGACCTC CGCTGATGCC 
TTCACCAATC TGGTGTCAAC CTACGGCGGC TATCACAGCT CCATTGACTA TCACAACGCC 
ATGACCCCGC CCAGTTCAGT GTCTCCCAGG GACTCGAATC AACCGGGTAA GGCGGCTCCA 
GTCCTGGCCT CCAACGGGGG ATACGACTAT GCCCCCGATC CACTCCGAGG GCAGTACGCC 
ACTTCATCCG GAGACGTGGT GCCCGCCACG CTGCCTCTGA AGCCCCAGGC ATCCTATACA 
GCAACAATGC ATCCTTCGGG CAGCACAACC ACCGAGGGTG GAGTTACCTA CAGTAATCTC 
GACCAGCCGC AGTACTTCGC TCCGCATTCG AGTTTCCACC TTTACCACAA GGGCAGCCCG 
GCCAGCGGCT GGTACTCCAC GCCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036575.1
CDS	654..3443
Translation	MLPYQAAVAMDYAGYQRQPTPGHPGSHMATMGSLGMPAVPFTHSWMVPTQDLCAMPPYNKMTGHQQPPGAGMHAQQQPLEPGILELRKEKSRDAARSRRGKENYEFYELAKMLPLPAAITSQLDKASIIRLTISYLKLRDFSGHGDPPWTREASSSSKLKSAAIRRSPAVDLFEQHQGTHILQSLDGFALAVAADGRFLYISETVSIYLGLSQVEMTGSSIFDYIHQADHSEIADQLGLSLTSGGGGGGGSSSSGGGGGGAGGGMASPTSGASDDGSGTHGTNNPDVAASMTQASTSGYKGYDRSFCVRMKSTLTKRGCHFKSSGYRVVLLLCKLRPQYTFSHSRKSQPPLLGMVALAIALPPPSVHEIRLECDMFVTRVNFDLRVAHCEPRVSDLLDYSPEDLVNKSLYSLCHAEDANRLRKSHSDLIEKGQVLTGYYRLMNKSGGYTWLQTCATVVCSTKNADEQNIICVNYVISNRENENMILDCCQLEPSPDSIKHEEGLGNDKSSGSPGGDASGEGNSHLSAGDMKLNSPKTDSEGHSHRGRGRSAAASHGSSMNSLTMIKDSPTPLGVEIDSGVLPTTVATPVPAATPPVQSTKRKRKTKASQHAEDQGQEQVISEQPLPKLPTMEQRDQQPRSRLPSIVDEQPSSAADSAVKDLEQAMSKHLPSPAAVVSVAPPNTDFSADSLLKQQQQQQQLDPNEKSSTIQWIGTPYQQPPAPMPATALLRQLYANRESVIRATARQTPTGVGPGVFYGDQQTGPLPTPPGSESSYENQYLQLHSAASGGHPGGQKTSADAFTNLVSTYGGYHSSIDYHNAMTPPSSVSPRDSNQPGKAAPVLASNGGYDYAPDPLRGQYATSSGDVVPATLPLKPQASYTATMHPSGSTTTEGGVTYSNLDQPQYFAPHSSFHLYHKGSPASGWYSTPS

Target ORF information:

RefSeq Version	NM_001043110.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster trachealess (trh), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043110.2

ORF Insert Sequence:

ATGTTGCCGT ACCAGGCAGC CGTGGCCATG GACTACGCCG GATACCAGAG ACAGCCCACG 
CCCGGACATC CCGGAAGCCA CATGGCCACC ATGGGATCTC TGGGCATGCC CGCGGTGCCC 
TTCACCCACA GCTGGATGGT GCCCACGCAG GACCTTTGCG CCATGCCACC CTACAACAAA 
ATGACCGGAC ACCAGCAGCC GCCAGGTGCC GGAATGCACG CCCAGCAACA GCCCCTCGAG 
CCCGGCATCC TGGAGCTGCG CAAGGAGAAG TCGAGGGACG CGGCGAGATC GCGGCGCGGA 
AAGGAGAACT ACGAGTTCTA CGAGCTAGCC AAGATGCTCC CTCTGCCGGC AGCCATCACC 
AGCCAGCTGG ACAAGGCCTC GATCATAAGA CTGACCATCA GCTACCTGAA GCTGAGGGAT 
TTCTCCGGAC ACGGCGATCC GCCATGGACT CGGGAAGCCT CTAGCAGCAG TAAGCTAAAA 
AGTGCCGCCA TTCGTCGCAG CCCCGCTGTT GATTTGTTCG AGCAACATCA GGGCACCCAC 
ATATTACAGT CGCTGGATGG TTTCGCCCTA GCTGTGGCGG CAGACGGACG CTTCCTGTAT 
ATTTCCGAGA CGGTGTCCAT CTATTTGGGC CTGTCGCAGG TGGAGATGAC GGGTAGCAGC 
ATCTTCGACT ATATCCACCA GGCGGATCAC TCGGAGATCG CCGACCAGCT GGGCCTGAGC 
CTAACAAGCG GCGGCGGCGG TGGCGGTGGA AGCTCGAGCA GCGGCGGAGG AGGAGGCGGA 
GCGGGAGGTG GTATGGCCTC ACCCACTTCC GGAGCCTCCG ACGATGGAAG CGGCACACAC 
GGTACGAACA ATCCCGACGT TGCGGCCTCC ATGACCCAAG CTTCGACCAG CGGCTACAAG 
GGATACGATC GAAGCTTCTG TGTCCGTATG AAGTCCACGC TGACCAAGCG CGGCTGTCAC 
TTCAAATCCT CAGGCTATCG GGTGGTTCTG CTGTTGTGCA AGCTGCGTCC CCAGTACACA 
TTTTCCCACA GTCGCAAATC GCAGCCTCCA CTCCTCGGAA TGGTTGCCCT GGCCATCGCA 
CTGCCTCCGC CATCGGTGCA CGAGATCCGG CTGGAGTGCG ACATGTTCGT CACCAGGGTG 
AACTTTGACC TGCGAGTAGC CCACTGCGAG CCAAGGGTAT CCGATCTGCT GGACTATTCC 
CCGGAGGACC TGGTTAACAA GAGCTTGTAC TCACTGTGTC ACGCCGAGGA TGCCAATCGC 
CTGCGCAAGA GCCACTCAGA CCTGATCGAG AAGGGTCAGG TGTTGACCGG ATACTACCGA 
CTAATGAACA AGAGTGGAGG CTACACTTGG CTCCAGACCT GTGCCACTGT CGTCTGCAGC 
ACAAAGAACG CCGATGAGCA GAACATCATC TGCGTGAACT ATGTGATCAG CAACCGGGAG 
AACGAGAACA TGATACTGGA TTGCTGCCAA CTGGAACCCA GTCCGGATAG CATTAAGCAC 
GAGGAGGGAC TGGGTAACGA CAAAAGCAGC GGGTCGCCAG GAGGAGATGC CAGTGGCGAA 
GGCAACTCTC ACCTGAGTGC CGGAGACATG AAGCTTAACT CACCGAAAAC CGATTCAGAG 
GGCCATTCCC ACCGCGGCAG GGGTCGCAGT GCAGCCGCCT CGCATGGAAG TTCAATGAAC 
AGCCTCACCA TGATCAAGGA TAGCCCCACT CCGCTGGGTG TGGAGATCGA TTCGGGAGTG 
CTGCCCACCA CAGTGGCCAC TCCAGTGCCG GCAGCCACTC CACCAGTGCA GTCCACCAAG 
CGAAAGCGAA AAACCAAAGC GTCGCAGCAC GCGGAGGACC AGGGTCAAGA GCAGGTAATC 
TCCGAGCAGC CTCTGCCCAA ACTACCGACA ATGGAGCAAC GCGATCAGCA GCCCCGCAGC 
CGCTTACCCT CGATTGTGGA TGAGCAGCCC TCGTCCGCGG CCGACTCCGC GGTCAAGGAT 
CTGGAGCAGG CCATGTCCAA GCATCTGCCC TCGCCAGCGG CGGTGGTGTC CGTGGCCCCG 
CCCAATACGG ATTTCAGTGC CGACTCTCTG CTTAAGCAGC AGCAACAACA ACAGCAGCTG 
GATCCCAACG AGAAAAGCAG CACCATTCAA TGGATAGGCA CTCCCTACCA ACAGCCACCG 
GCGCCCATGC CAGCTACCGC CCTTCTCCGG CAGTTATACG CCAACCGGGA GAGCGTGATC 
CGGGCGACGG CTAGGCAAAC ACCAACTGGA GTGGGCCCCG GAGTTTTCTA CGGCGATCAG 
CAAACAGGTC CGCTGCCCAC TCCGCCGGGC AGTGAGTCGT CCTACGAGAA CCAATACCTG 
CAGCTGCACT CTGCCGCTTC CGGTGGACAT CCTGGCGGCC AGAAGACCTC CGCTGATGCC 
TTCACCAATC TGGTGTCAAC CTACGGCGGC TATCACAGCT CCATTGACTA TCACAACGCC 
ATGACCCCGC CCAGTTCAGT GTCTCCCAGG GACTCGAATC AACCGGGTAA GGCGGCTCCA 
GTCCTGGCCT CCAACGGGGG ATACGACTAT GCCCCCGATC CACTCCGAGG GCAGTACGCC 
ACTTCATCCG GAGACGTGGT GCCCGCCACG CTGCCTCTGA AGCCCCAGGC ATCCTATACA 
GCAACAATGC ATCCTTCGGG CAGCACAACC ACCGAGGGTG GAGTTACCTA CAGTAATCTC 
GACCAGCCGC AGTACTTCGC TCCGCATTCG AGTTTCCACC TTTACCACAA GGGCAGCCCG 
GCCAGCGGCT GGTACTCCAC GCCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trh ( NM_079148.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523872.2 Drosophila melanogaster trachealess (trh), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa20355
Clone ID Related Accession (Same CDS sequence)	NM_079148.3
Accession Version	NM_079148.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	trachealess, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Dec 17, 2009 this sequence version replaced NM_079148.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCCGT ACCAGGCAGC CGTGGCCATG GACTACGCCG GATACCAGAG ACAGCCCACG 
CCCGGACATC CCGGAAGCCA CATGGCCACC ATGGGATCTC TGGGCATGCC CGCGGTGCCC 
TTCACCCACA GCTGGATGGT GCCCACGCAG GACCTTTGCG CCATGCCACC CTACAACAAA 
ATGACCGGAC ACCAGCAGCC GCCAGGTGCC GGAATGCACG CCCAGCAACA GCCCCTCGAG 
CCCGGCATCC TGGAGCTGCG CAAGGAGAAG TCGAGGGACG CGGCGAGATC GCGGCGCGGA 
AAGGAGAACT ACGAGTTCTA CGAGCTAGCC AAGATGCTCC CTCTGCCGGC AGCCATCACC 
AGCCAGCTGG ACAAGGCCTC GATCATAAGA CTGACCATCA GCTACCTGAA GCTGAGGGAT 
TTCTCCGGAC ACGGCGATCC GCCATGGACT CGGGAAGCCT CTAGCAGCAG TAAGCTAAAA 
AGTGCCGCCA TTCGTCGCAG CCCCGCTGTT GATTTGTTCG AGCAACATCA GGGCACCCAC 
ATATTACAGT CGCTGGATGG TTTCGCCCTA GCTGTGGCGG CAGACGGACG CTTCCTGTAT 
ATTTCCGAGA CGGTGTCCAT CTATTTGGGC CTGTCGCAGG TGGAGATGAC GGGTAGCAGC 
ATCTTCGACT ATATCCACCA GGCGGATCAC TCGGAGATCG CCGACCAGCT GGGCCTGAGC 
CTAACAAGCG GCGGCGGCGG TGGCGGTGGA AGCTCGAGCA GCGGCGGAGG AGGAGGCGGA 
GCGGGAGGTG GTATGGCCTC ACCCACTTCC GGAGCCTCCG ACGATGGAAG CGGCACACAC 
GGTACGAACA ATCCCGACGT TGCGGCCTCC ATGACCCAAG CTTCGACCAG CGGCTACAAG 
GGATACGATC GAAGCTTCTG TGTCCGTATG AAGTCCACGC TGACCAAGCG CGGCTGTCAC 
TTCAAATCCT CAGGCTATCG GGCCAGCGAT GCAACGAGCA ATTGCAACAA CGGTAACAAT 
GCTAGTAACA ATGCTAAAAA CGTTAAGAAT CCGGGCTCAA ACTATTCGGT GGTTCTGCTG 
TTGTGCAAGC TGCGTCCCCA GTACACATTT TCCCACAGTC GCAAATCGCA GCCTCCACTC 
CTCGGAATGG TTGCCCTGGC CATCGCACTG CCTCCGCCAT CGGTGCACGA GATCCGGCTG 
GAGTGCGACA TGTTCGTCAC CAGGGTGAAC TTTGACCTGC GAGTAGCCCA CTGCGAGCCA 
AGGGTATCCG ATCTGCTGGA CTATTCCCCG GAGGACCTGG TTAACAAGAG CTTGTACTCA 
CTGTGTCACG CCGAGGATGC CAATCGCCTG CGCAAGAGCC ACTCAGACCT GATCGAGAAG 
GGTCAGGTGT TGACCGGATA CTACCGACTA ATGAACAAGA GTGGAGGCTA CACTTGGCTC 
CAGACCTGTG CCACTGTCGT CTGCAGCACA AAGAACGCCG ATGAGCAGAA CATCATCTGC 
GTGAACTATG TGATCAGCAA CCGGGAGAAC GAGAACATGA TACTGGATTG CTGCCAACTG 
GAACCCAGTC CGGATAGCAT TAAGCACGAG GAGGGACTGG GTAACGACAA AAGCAGCGGG 
TCGCCAGGAG GAGATGCCAG TGGCGAAGGC AACTCTCACC TGAGTGCCGG AGACATGAAG 
CTTAACTCAC CGAAAACCGA TTCAGAGGGC CATTCCCACC GCGGCAGGGG TCGCAGTGCA 
GCCGCCTCGC ATGGAAGTTC AATGAACAGC CTCACCATGA TCAAGGATAG CCCCACTCCG 
CTGGGTGTGG AGATCGATTC GGGAGTGCTG CCCACCACAG TGGCCACTCC AGTGCCGGCA 
GCCACTCCAC CAGTGCAGTC CACCAAGCGA AAGCGAAAAA CCAAAGCGTC GCAGCACGCG 
GAGGACCAGG GTCAAGAGCA GGTAATCTCC GAGCAGCCTC TGCCCAAACT ACCGACAATG 
GAGCAACGCG ATCAGCAGCC CCGCAGCCGC TTACCCTCGA TTGTGGATGA GCAGCCCTCG 
TCCGCGGCCG ACTCCGCGGT CAAGGATCTG GAGCAGGCCA TGTCCAAGCA TCTGCCCTCG 
CCAGCGGCGG TGGTGTCCGT GGCCCCGCCC AATACGGATT TCAGTGCCGA CTCTCTGCTT 
AAGCAGCAGC AACAACAACA GCAGCTGGAT CCCAACGAGA AAAGCAGCAC CATTCAATGG 
ATAGGCACTC CCTACCAACA GCCACCGGCG CCCATGCCAG CTACCGCCCT TCTCCGGCAG 
TTATACGCCA ACCGGGAGAG CGTGATCCGG GCGACGGCTA GGCAAACACC AACTGGAGTG 
GGCCCCGGAG TTTTCTACGG CGATCAGCAA ACAGGTCCGC TGCCCACTCC GCCGGGCAGT 
GAGTCGTCCT ACGAGAACCA ATACCTGCAG CTGCACTCTG CCGCTTCCGG TGGACATCCT 
GGCGGCCAGA AGACCTCCGC TGATGCCTTC ACCAATCTGG TGTCAACCTA CGGCGGCTAT 
CACAGCTCCA TTGACTATCA CAACGCCATG ACCCCGCCCA GTTCAGTGTC TCCCAGGGAC 
TCGAATCAAC CGGGTAAGGC GGCTCCAGTC CTGGCCTCCA ACGGGGGATA CGACTATGCC 
CCCGATCCAC TCCGAGGGCA GTACGCCACT TCATCCGGAG ACGTGGTGCC CGCCACGCTG 
CCTCTGAAGC CCCAGGCATC CTATACAGCA ACAATGCATC CTTCGGGCAG CACAACCACC 
GAGGGTGGAG TTACCTACAG TAATCTCGAC CAGCCGCAGT ACTTCGCTCC GCATTCGAGT 
TTCCACCTTT ACCACAAGGG CAGCCCGGCC AGCGGCTGGT ACTCCACGCC CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523872.2
CDS	654..3530
Translation	MLPYQAAVAMDYAGYQRQPTPGHPGSHMATMGSLGMPAVPFTHSWMVPTQDLCAMPPYNKMTGHQQPPGAGMHAQQQPLEPGILELRKEKSRDAARSRRGKENYEFYELAKMLPLPAAITSQLDKASIIRLTISYLKLRDFSGHGDPPWTREASSSSKLKSAAIRRSPAVDLFEQHQGTHILQSLDGFALAVAADGRFLYISETVSIYLGLSQVEMTGSSIFDYIHQADHSEIADQLGLSLTSGGGGGGGSSSSGGGGGGAGGGMASPTSGASDDGSGTHGTNNPDVAASMTQASTSGYKGYDRSFCVRMKSTLTKRGCHFKSSGYRASDATSNCNNGNNASNNAKNVKNPGSNYSVVLLLCKLRPQYTFSHSRKSQPPLLGMVALAIALPPPSVHEIRLECDMFVTRVNFDLRVAHCEPRVSDLLDYSPEDLVNKSLYSLCHAEDANRLRKSHSDLIEKGQVLTGYYRLMNKSGGYTWLQTCATVVCSTKNADEQNIICVNYVISNRENENMILDCCQLEPSPDSIKHEEGLGNDKSSGSPGGDASGEGNSHLSAGDMKLNSPKTDSEGHSHRGRGRSAAASHGSSMNSLTMIKDSPTPLGVEIDSGVLPTTVATPVPAATPPVQSTKRKRKTKASQHAEDQGQEQVISEQPLPKLPTMEQRDQQPRSRLPSIVDEQPSSAADSAVKDLEQAMSKHLPSPAAVVSVAPPNTDFSADSLLKQQQQQQQLDPNEKSSTIQWIGTPYQQPPAPMPATALLRQLYANRESVIRATARQTPTGVGPGVFYGDQQTGPLPTPPGSESSYENQYLQLHSAASGGHPGGQKTSADAFTNLVSTYGGYHSSIDYHNAMTPPSSVSPRDSNQPGKAAPVLASNGGYDYAPDPLRGQYATSSGDVVPATLPLKPQASYTATMHPSGSTTTEGGVTYSNLDQPQYFAPHSSFHLYHKGSPASGWYSTPS

Target ORF information:

RefSeq Version	NM_079148.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster trachealess (trh), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079148.3

ORF Insert Sequence:

ATGTTGCCGT ACCAGGCAGC CGTGGCCATG GACTACGCCG GATACCAGAG ACAGCCCACG 
CCCGGACATC CCGGAAGCCA CATGGCCACC ATGGGATCTC TGGGCATGCC CGCGGTGCCC 
TTCACCCACA GCTGGATGGT GCCCACGCAG GACCTTTGCG CCATGCCACC CTACAACAAA 
ATGACCGGAC ACCAGCAGCC GCCAGGTGCC GGAATGCACG CCCAGCAACA GCCCCTCGAG 
CCCGGCATCC TGGAGCTGCG CAAGGAGAAG TCGAGGGACG CGGCGAGATC GCGGCGCGGA 
AAGGAGAACT ACGAGTTCTA CGAGCTAGCC AAGATGCTCC CTCTGCCGGC AGCCATCACC 
AGCCAGCTGG ACAAGGCCTC GATCATAAGA CTGACCATCA GCTACCTGAA GCTGAGGGAT 
TTCTCCGGAC ACGGCGATCC GCCATGGACT CGGGAAGCCT CTAGCAGCAG TAAGCTAAAA 
AGTGCCGCCA TTCGTCGCAG CCCCGCTGTT GATTTGTTCG AGCAACATCA GGGCACCCAC 
ATATTACAGT CGCTGGATGG TTTCGCCCTA GCTGTGGCGG CAGACGGACG CTTCCTGTAT 
ATTTCCGAGA CGGTGTCCAT CTATTTGGGC CTGTCGCAGG TGGAGATGAC GGGTAGCAGC 
ATCTTCGACT ATATCCACCA GGCGGATCAC TCGGAGATCG CCGACCAGCT GGGCCTGAGC 
CTAACAAGCG GCGGCGGCGG TGGCGGTGGA AGCTCGAGCA GCGGCGGAGG AGGAGGCGGA 
GCGGGAGGTG GTATGGCCTC ACCCACTTCC GGAGCCTCCG ACGATGGAAG CGGCACACAC 
GGTACGAACA ATCCCGACGT TGCGGCCTCC ATGACCCAAG CTTCGACCAG CGGCTACAAG 
GGATACGATC GAAGCTTCTG TGTCCGTATG AAGTCCACGC TGACCAAGCG CGGCTGTCAC 
TTCAAATCCT CAGGCTATCG GGCCAGCGAT GCAACGAGCA ATTGCAACAA CGGTAACAAT 
GCTAGTAACA ATGCTAAAAA CGTTAAGAAT CCGGGCTCAA ACTATTCGGT GGTTCTGCTG 
TTGTGCAAGC TGCGTCCCCA GTACACATTT TCCCACAGTC GCAAATCGCA GCCTCCACTC 
CTCGGAATGG TTGCCCTGGC CATCGCACTG CCTCCGCCAT CGGTGCACGA GATCCGGCTG 
GAGTGCGACA TGTTCGTCAC CAGGGTGAAC TTTGACCTGC GAGTAGCCCA CTGCGAGCCA 
AGGGTATCCG ATCTGCTGGA CTATTCCCCG GAGGACCTGG TTAACAAGAG CTTGTACTCA 
CTGTGTCACG CCGAGGATGC CAATCGCCTG CGCAAGAGCC ACTCAGACCT GATCGAGAAG 
GGTCAGGTGT TGACCGGATA CTACCGACTA ATGAACAAGA GTGGAGGCTA CACTTGGCTC 
CAGACCTGTG CCACTGTCGT CTGCAGCACA AAGAACGCCG ATGAGCAGAA CATCATCTGC 
GTGAACTATG TGATCAGCAA CCGGGAGAAC GAGAACATGA TACTGGATTG CTGCCAACTG 
GAACCCAGTC CGGATAGCAT TAAGCACGAG GAGGGACTGG GTAACGACAA AAGCAGCGGG 
TCGCCAGGAG GAGATGCCAG TGGCGAAGGC AACTCTCACC TGAGTGCCGG AGACATGAAG 
CTTAACTCAC CGAAAACCGA TTCAGAGGGC CATTCCCACC GCGGCAGGGG TCGCAGTGCA 
GCCGCCTCGC ATGGAAGTTC AATGAACAGC CTCACCATGA TCAAGGATAG CCCCACTCCG 
CTGGGTGTGG AGATCGATTC GGGAGTGCTG CCCACCACAG TGGCCACTCC AGTGCCGGCA 
GCCACTCCAC CAGTGCAGTC CACCAAGCGA AAGCGAAAAA CCAAAGCGTC GCAGCACGCG 
GAGGACCAGG GTCAAGAGCA GGTAATCTCC GAGCAGCCTC TGCCCAAACT ACCGACAATG 
GAGCAACGCG ATCAGCAGCC CCGCAGCCGC TTACCCTCGA TTGTGGATGA GCAGCCCTCG 
TCCGCGGCCG ACTCCGCGGT CAAGGATCTG GAGCAGGCCA TGTCCAAGCA TCTGCCCTCG 
CCAGCGGCGG TGGTGTCCGT GGCCCCGCCC AATACGGATT TCAGTGCCGA CTCTCTGCTT 
AAGCAGCAGC AACAACAACA GCAGCTGGAT CCCAACGAGA AAAGCAGCAC CATTCAATGG 
ATAGGCACTC CCTACCAACA GCCACCGGCG CCCATGCCAG CTACCGCCCT TCTCCGGCAG 
TTATACGCCA ACCGGGAGAG CGTGATCCGG GCGACGGCTA GGCAAACACC AACTGGAGTG 
GGCCCCGGAG TTTTCTACGG CGATCAGCAA ACAGGTCCGC TGCCCACTCC GCCGGGCAGT 
GAGTCGTCCT ACGAGAACCA ATACCTGCAG CTGCACTCTG CCGCTTCCGG TGGACATCCT 
GGCGGCCAGA AGACCTCCGC TGATGCCTTC ACCAATCTGG TGTCAACCTA CGGCGGCTAT 
CACAGCTCCA TTGACTATCA CAACGCCATG ACCCCGCCCA GTTCAGTGTC TCCCAGGGAC 
TCGAATCAAC CGGGTAAGGC GGCTCCAGTC CTGGCCTCCA ACGGGGGATA CGACTATGCC 
CCCGATCCAC TCCGAGGGCA GTACGCCACT TCATCCGGAG ACGTGGTGCC CGCCACGCTG 
CCTCTGAAGC CCCAGGCATC CTATACAGCA ACAATGCATC CTTCGGGCAG CACAACCACC 
GAGGGTGGAG TTACCTACAG TAATCTCGAC CAGCCGCAGT ACTTCGCTCC GCATTCGAGT 
TTCCACCTTT ACCACAAGGG CAGCCCGGCC AGCGGCTGGT ACTCCACGCC CTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trh ( NM_001274278.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261207.1 Drosophila melanogaster trachealess (trh), transcript variant D, mRNA.

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CloneID	OFa17072
Clone ID Related Accession (Same CDS sequence)	NM_001274278.1
Accession Version	NM_001274278.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	trachealess, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCCGT ACCAGGCAGC CGTGGCCATG GACTACGCCG GATACCAGAG ACAGCCCACG 
CCCGGACATC CCGGAAGCCA CATGGCCACC ATGGGATCTC TGGGCATGCC CGCGGTGCCC 
TTCACCCACA GCTGGATGGT GCCCACGCAG GACCTTTGCG CCATGCCACC CTACAACAAA 
ATGACCGGAC ACCAGCAGCC GCCAGGTGCC GGAATGCACG CCCAGCAACA GCCCCTCGAG 
CCCGGCATCC TGGAGCTGCG CAAGGAGAAG TCGAGGGACG CGGCGAGATC GCGGCGCGGA 
AAGGAGAACT ACGAGTTCTA CGAGCTAGCC AAGATGCTCC CTCTGCCGGC AGCCATCACC 
AGCCAGCTGG ACAAGGCCTC GATCATAAGA CTGACCATCA GCTACCTGAA GCTGAGGGAT 
TTCTCCGGAC ACGGCGATCC GCCATGGACT CGGGAAGCCT CTAGCAGCAG TAAGCTAAAA 
AGTGCCGCCA TTCGTCGCAG CCCCGCTGTT GATTTGTTCG AGCAACATCA GGGCACCCAC 
ATATTACAGT CGCTGGATGG TTTCGCCCTA GCTGTGGCGG CAGACGGACG CTTCCTGTAT 
ATTTCCGAGA CGGTGTCCAT CTATTTGGGC CTGTCGCAGG TGGAGATGAC GGGTAGCAGC 
ATCTTCGACT ATATCCACCA GGCGGATCAC TCGGAGATCG CCGACCAGCT GGGCCTGAGC 
CTAACAAGCG GCGGCGGCGG TGGCGGTGGA AGCTCGAGCA GCGGCGGAGG AGGAGGCGGA 
GCGGGAGGTG GTATGGCCTC ACCCACTTCC GGAGCCTCCG ACGATGGAAG CGGCACACAC 
GGTACGAACA ATCCCGACGT TGCGGCCTCC ATGACCCAAG CTTCGACCAG CGGCTACAAG 
GGATACGATC GAAGCTTCTG TGTCCGTATG AAGTCCACGC TGACCAAGCG CGGCTGTCAC 
TTCAAATCCT CAGGCTATCG GGCCAGCGAT GCAACGAGCA ATTGCAACAA CGGTAACAAT 
GCTAGTAACA ATGCTAAAAA CGTTAAGAAT CCGGGCTCAA ACTATTCGGT GGTTCTGCTG 
TTGTGCAAGC TGCGTCCCCA GTACACATTT TCCCACAGTC GCAAATCGCA GCCTCCACTC 
CTCGGAATGG TTGCCCTGGC CATCGCACTG CCTCCGCCAT CGGTGCACGA GATCCGGCTG 
GAGTGCGACA TGTTCGTCAC CAGGGTGAAC TTTGACCTGC GAGTAGCCCA CTGCGAGCCA 
AGGGTATCCG ATCTGCTGGA CTATTCCCCG GAGGACCTGG TTAACAAGAG CTTGTACTCA 
CTGTGTCACG CCGAGGATGC CAATCGCCTG CGCAAGAGCC ACTCAGACCT GATCGAGAAG 
GGTCAGGTGT TGACCGGATA CTACCGACTA ATGAACAAGA GTGGAGGCTA CACTTGGCTC 
CAGACCTGTG CCACTGTCGT CTGCAGCACA AAGAACGCCG ATGAGCAGAA CATCATCTGC 
GTGAACTATG TGATCAGCAA CCGGGAGAAC GAGAACATGA TACTGGATTG CTGCCAACTG 
GAACCCAGTC CGGATAGCAT TAAGCACGAG GAGGGACTGG GTAACGACAA AAGCAGCGGG 
TCGCCAGGAG GAGATGCCAG TGGCGAAGGC AACTCTCACC TGAGTGCCGG AGACATGAAG 
CTTAACTCAC CGAAAACCGA TTCAGAGGGC CATTCCCACC GCGGCAGGGG TCGCAGTGCA 
GCCGCCTCGC ATGGAAGTTC AATGAACAGC CTCACCATGA TCAAGGATAG CCCCACTCCG 
CTGGGTGTGG AGATCGATTC GGGAGTGCTG CCCACCACAG TGGCCACTCC AGTGCCGGCA 
GCCACTCCAC CAGTGCAGTC CACCAAGCGA AAGCGAAAAA CCAAAGCGTC GCAGCACGCG 
GAGGACCAGG GTCAAGAGCA GGTAATCTCC GAGCAGCCTC TGCCCAAACT ACCGACAATG 
GAGCAACGCG ATCAGCAGCC CCGCAGCCGC TTACCCTCGA TTGTGGATGA GCAGCCCTCG 
TCCGCGGCCG ACTCCGCGGT CAAGGATCTG GAGCAGGCCA TGTCCAAGCA TCTGCCCTCG 
CCAGCGGCGG TGGTGTCCGT GGCCCCGCCC AATACGGATT TCAGTGCCGA CTCTCTGCTT 
AAGCAGCAGC AACAACAACA GCAGCTGGAT CCCAACGAGA AAAGCAGCAC CATTCAATGG 
ATAGGCACTC CCTACCAACA GCCACCGGCG CCCATGCCAG CTACCGCCCT TCTCCGGCAG 
TTATACGCCA ACCGGGAGAG CGTGATCCGG GCGACGGCTA GGCAAACACC AACTGGAGTG 
GGCCCCGGAG TTTTCTACGG CGATCAGCAA ACAGGTCCGC TGCCCACTCC GCCGGGCAGT 
GAGTCGTCCT ACGAGAACCA ATACCTGCAG CTGCACTCTG CCGCTTCCGG TGGACATCCT 
GGCGGCCAGA AGACCTCCGC TGATGCCTTC ACCAATCTGG TGTCAACCTA CGGCGGCTAT 
CACAGCTCCA TTGACTATCA CAACGCCATG ACCCCGCCCA GTTCAGTGTC TCCCAGGGAC 
TCGAATCAAC CGGGTAAGGC GGCTCCAGTC CTGGCCTCCA ACGGGGGATA CGACTATGCC 
CCCGATCCAC TCCGAGGGCA GTACGCCACT TCATCCGGAG ACGTGGTGCC CGCCACGCTG 
CCTCTGAAGC CCCAGGCATC CTATACAGCA ACAATGCATC CTTCGGGCAG CACAACCACC 
GAGGGTGGAG TTACCTACAG TAATCTCGAC CAGCCGCAGT ACTTCGCTCC GCATTCGAGT 
TTCCACCTTT ACCACAAGGG CAGCCCGGCC AGCGGCTGGT ACTCCACGCC CTCCTAGGTG 
GTAGACGACC AGGGGCAGGT CCCGCCATCC TGTCAGGACC AGTACCACCA CCACCACCAC 
CACCACCATC ATCAAGACGG ATCAGCGGGA TCCAGTGCAA GCCAGGCGAG CGAGCGATGG 
GACTTTGTGG GTGCGCTGGG CAAGGTGGCC AGGATGTTTT TTAGCGCCAG AAAGGGTAAT 
CCGGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261207.1
CDS	654..3722
Translation	MLPYQAAVAMDYAGYQRQPTPGHPGSHMATMGSLGMPAVPFTHSWMVPTQDLCAMPPYNKMTGHQQPPGAGMHAQQQPLEPGILELRKEKSRDAARSRRGKENYEFYELAKMLPLPAAITSQLDKASIIRLTISYLKLRDFSGHGDPPWTREASSSSKLKSAAIRRSPAVDLFEQHQGTHILQSLDGFALAVAADGRFLYISETVSIYLGLSQVEMTGSSIFDYIHQADHSEIADQLGLSLTSGGGGGGGSSSSGGGGGGAGGGMASPTSGASDDGSGTHGTNNPDVAASMTQASTSGYKGYDRSFCVRMKSTLTKRGCHFKSSGYRASDATSNCNNGNNASNNAKNVKNPGSNYSVVLLLCKLRPQYTFSHSRKSQPPLLGMVALAIALPPPSVHEIRLECDMFVTRVNFDLRVAHCEPRVSDLLDYSPEDLVNKSLYSLCHAEDANRLRKSHSDLIEKGQVLTGYYRLMNKSGGYTWLQTCATVVCSTKNADEQNIICVNYVISNRENENMILDCCQLEPSPDSIKHEEGLGNDKSSGSPGGDASGEGNSHLSAGDMKLNSPKTDSEGHSHRGRGRSAAASHGSSMNSLTMIKDSPTPLGVEIDSGVLPTTVATPVPAATPPVQSTKRKRKTKASQHAEDQGQEQVISEQPLPKLPTMEQRDQQPRSRLPSIVDEQPSSAADSAVKDLEQAMSKHLPSPAAVVSVAPPNTDFSADSLLKQQQQQQQLDPNEKSSTIQWIGTPYQQPPAPMPATALLRQLYANRESVIRATARQTPTGVGPGVFYGDQQTGPLPTPPGSESSYENQYLQLHSAASGGHPGGQKTSADAFTNLVSTYGGYHSSIDYHNAMTPPSSVSPRDSNQPGKAAPVLASNGGYDYAPDPLRGQYATSSGDVVPATLPLKPQASYTATMHPSGSTTTEGGVTYSNLDQPQYFAPHSSFHLYHKGSPASGWYSTPS*VVDDQGQVPPSCQDQYHHHHHHHHHQDGSAGSSASQASERWDFVGALGKVARMFFSARKGNPG

Target ORF information:

RefSeq Version	NM_001274278.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster trachealess (trh), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274278.1

ORF Insert Sequence:

ATGTTGCCGT ACCAGGCAGC CGTGGCCATG GACTACGCCG GATACCAGAG ACAGCCCACG 
CCCGGACATC CCGGAAGCCA CATGGCCACC ATGGGATCTC TGGGCATGCC CGCGGTGCCC 
TTCACCCACA GCTGGATGGT GCCCACGCAG GACCTTTGCG CCATGCCACC CTACAACAAA 
ATGACCGGAC ACCAGCAGCC GCCAGGTGCC GGAATGCACG CCCAGCAACA GCCCCTCGAG 
CCCGGCATCC TGGAGCTGCG CAAGGAGAAG TCGAGGGACG CGGCGAGATC GCGGCGCGGA 
AAGGAGAACT ACGAGTTCTA CGAGCTAGCC AAGATGCTCC CTCTGCCGGC AGCCATCACC 
AGCCAGCTGG ACAAGGCCTC GATCATAAGA CTGACCATCA GCTACCTGAA GCTGAGGGAT 
TTCTCCGGAC ACGGCGATCC GCCATGGACT CGGGAAGCCT CTAGCAGCAG TAAGCTAAAA 
AGTGCCGCCA TTCGTCGCAG CCCCGCTGTT GATTTGTTCG AGCAACATCA GGGCACCCAC 
ATATTACAGT CGCTGGATGG TTTCGCCCTA GCTGTGGCGG CAGACGGACG CTTCCTGTAT 
ATTTCCGAGA CGGTGTCCAT CTATTTGGGC CTGTCGCAGG TGGAGATGAC GGGTAGCAGC 
ATCTTCGACT ATATCCACCA GGCGGATCAC TCGGAGATCG CCGACCAGCT GGGCCTGAGC 
CTAACAAGCG GCGGCGGCGG TGGCGGTGGA AGCTCGAGCA GCGGCGGAGG AGGAGGCGGA 
GCGGGAGGTG GTATGGCCTC ACCCACTTCC GGAGCCTCCG ACGATGGAAG CGGCACACAC 
GGTACGAACA ATCCCGACGT TGCGGCCTCC ATGACCCAAG CTTCGACCAG CGGCTACAAG 
GGATACGATC GAAGCTTCTG TGTCCGTATG AAGTCCACGC TGACCAAGCG CGGCTGTCAC 
TTCAAATCCT CAGGCTATCG GGCCAGCGAT GCAACGAGCA ATTGCAACAA CGGTAACAAT 
GCTAGTAACA ATGCTAAAAA CGTTAAGAAT CCGGGCTCAA ACTATTCGGT GGTTCTGCTG 
TTGTGCAAGC TGCGTCCCCA GTACACATTT TCCCACAGTC GCAAATCGCA GCCTCCACTC 
CTCGGAATGG TTGCCCTGGC CATCGCACTG CCTCCGCCAT CGGTGCACGA GATCCGGCTG 
GAGTGCGACA TGTTCGTCAC CAGGGTGAAC TTTGACCTGC GAGTAGCCCA CTGCGAGCCA 
AGGGTATCCG ATCTGCTGGA CTATTCCCCG GAGGACCTGG TTAACAAGAG CTTGTACTCA 
CTGTGTCACG CCGAGGATGC CAATCGCCTG CGCAAGAGCC ACTCAGACCT GATCGAGAAG 
GGTCAGGTGT TGACCGGATA CTACCGACTA ATGAACAAGA GTGGAGGCTA CACTTGGCTC 
CAGACCTGTG CCACTGTCGT CTGCAGCACA AAGAACGCCG ATGAGCAGAA CATCATCTGC 
GTGAACTATG TGATCAGCAA CCGGGAGAAC GAGAACATGA TACTGGATTG CTGCCAACTG 
GAACCCAGTC CGGATAGCAT TAAGCACGAG GAGGGACTGG GTAACGACAA AAGCAGCGGG 
TCGCCAGGAG GAGATGCCAG TGGCGAAGGC AACTCTCACC TGAGTGCCGG AGACATGAAG 
CTTAACTCAC CGAAAACCGA TTCAGAGGGC CATTCCCACC GCGGCAGGGG TCGCAGTGCA 
GCCGCCTCGC ATGGAAGTTC AATGAACAGC CTCACCATGA TCAAGGATAG CCCCACTCCG 
CTGGGTGTGG AGATCGATTC GGGAGTGCTG CCCACCACAG TGGCCACTCC AGTGCCGGCA 
GCCACTCCAC CAGTGCAGTC CACCAAGCGA AAGCGAAAAA CCAAAGCGTC GCAGCACGCG 
GAGGACCAGG GTCAAGAGCA GGTAATCTCC GAGCAGCCTC TGCCCAAACT ACCGACAATG 
GAGCAACGCG ATCAGCAGCC CCGCAGCCGC TTACCCTCGA TTGTGGATGA GCAGCCCTCG 
TCCGCGGCCG ACTCCGCGGT CAAGGATCTG GAGCAGGCCA TGTCCAAGCA TCTGCCCTCG 
CCAGCGGCGG TGGTGTCCGT GGCCCCGCCC AATACGGATT TCAGTGCCGA CTCTCTGCTT 
AAGCAGCAGC AACAACAACA GCAGCTGGAT CCCAACGAGA AAAGCAGCAC CATTCAATGG 
ATAGGCACTC CCTACCAACA GCCACCGGCG CCCATGCCAG CTACCGCCCT TCTCCGGCAG 
TTATACGCCA ACCGGGAGAG CGTGATCCGG GCGACGGCTA GGCAAACACC AACTGGAGTG 
GGCCCCGGAG TTTTCTACGG CGATCAGCAA ACAGGTCCGC TGCCCACTCC GCCGGGCAGT 
GAGTCGTCCT ACGAGAACCA ATACCTGCAG CTGCACTCTG CCGCTTCCGG TGGACATCCT 
GGCGGCCAGA AGACCTCCGC TGATGCCTTC ACCAATCTGG TGTCAACCTA CGGCGGCTAT 
CACAGCTCCA TTGACTATCA CAACGCCATG ACCCCGCCCA GTTCAGTGTC TCCCAGGGAC 
TCGAATCAAC CGGGTAAGGC GGCTCCAGTC CTGGCCTCCA ACGGGGGATA CGACTATGCC 
CCCGATCCAC TCCGAGGGCA GTACGCCACT TCATCCGGAG ACGTGGTGCC CGCCACGCTG 
CCTCTGAAGC CCCAGGCATC CTATACAGCA ACAATGCATC CTTCGGGCAG CACAACCACC 
GAGGGTGGAG TTACCTACAG TAATCTCGAC CAGCCGCAGT ACTTCGCTCC GCATTCGAGT 
TTCCACCTTT ACCACAAGGG CAGCCCGGCC AGCGGCTGGT ACTCCACGCC CTCCTAGGTG 
GTAGACGACC AGGGGCAGGT CCCGCCATCC TGTCAGGACC AGTACCACCA CCACCACCAC 
CACCACCATC ATCAAGACGG ATCAGCGGGA TCCAGTGCAA GCCAGGCGAG CGAGCGATGG 
GACTTTGTGG GTGCGCTGGG CAAGGTGGCC AGGATGTTTT TTAGCGCCAG AAAGGGTAAT 
CCGGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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trh cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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