Dic61B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dic61B
Entrez Gene ID	38020
Full Name	Dynein intermediate chain at 61B
Synonyms	CG7051,Dmel\CG7051,ms,ms(3)21
General protein information	Preferred Names Dynein intermediate chain at 61B Names CG7051 gene product from transcript CG7051-RA CG7051-PA CG7051-PB Dic61B-PA Dic61B-PB dynein intermediate chain at 61B male sterile (3) 21
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 61B1-61B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_167796.3	NP_728477.1	dynein intermediate chain at 61B, isoform A
NM_138157.3	NP_612001.1	dynein intermediate chain at 61B, isoform B

Pathways from BioSystems

Homologies

	LOC699921	XP_002808265.1
Danio rerio (zebrafish)	wdr78	NP_001018595.1
Drosophila melanogaster (fruit fly)	Dic61B	NP_612001.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006036	XP_001237748.2
Xenopus tropicalis (tropical clawed frog)	wdr78	NP_001106504.1
Homo sapiens (human)	WDR78	NP_079039.4
Gallus gallus (chicken)	WDR78	XP_004936929.1
Pan troglodytes (chimpanzee)	WDR78	XP_003308226.1
Canis lupus familiaris (dog)	WDR78	XP_536676.3
Bos taurus (cattle)	WDR78	XP_002686378.3
Mus musculus (house mouse)	Wdr78	NP_666366.4
Rattus norvegicus (Norway rat)	Wdr78	NP_001019957.1
Drosophila melanogaster (fruit fly)	CG13930	NP_647645.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dic61B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dic61B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17028	NM_167796.3 Latest version!	Drosophila melanogaster dynein intermediate chain at 61B, transcript variant A (Dic61B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa17029	NM_138157.3 Latest version!	Drosophila melanogaster dynein intermediate chain at 61B, transcript variant B (Dic61B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dic61B ( NM_167796.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728477.1 Drosophila melanogaster dynein intermediate chain at 61B, transcript variant A (Dic61B), mRNA.

Price & Availability↑

CloneID	OFa17028
Clone ID Related Accession (Same CDS sequence)	NM_167796.3
Accession Version	NM_167796.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dynein intermediate chain at 61B, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Dec 17, 2009 this sequence version replaced NM_167796.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGCGT CAGTAAGAAA TACAGTCACT AGCCAAGAAA ATGGGAGTAT CTTTAAACGG 
TCCAGTAACT TAGACAACGA ACTCAAAAAG AAGTCCGTGG TGGTTAATCA GACACAAGCC 
GAAGAGTCTA AGGCTAAGAA GAAAGATACT ACGCACTATT ACAGTGTGGA AACACTTGCC 
GACCTGCAGT ATCGATCTCA GATAAAGGTT TTGGAAAAGA TCTATGGCGA GGAGGTTGAT 
ATGACACCTA AGTCGATCCA AGATGACACC TATGTGGAGC AGTTCATGAA GTACAACCTA 
AATGTCATGC ACCAAATGAC TAACTTCACT ACAATGGTAG CGAAGAGCAC GACCAGATCC 
CGCACGCGGT CTAGTGTTCG GGCCGGAATA GGCGGCAATA AGCAGTCCAT TGACGACCTG 
ATGAACGGGA TACTTTATGA AACTATTAAC TGGGAACCGT ACAGCACTGT TGAGGCTCTC 
GAAGAGGCGT ACGAGCCAAA GCCGTATACC AAGTCCTTTT TAAAGATCAC CTTACGCAAG 
TCTGACTTCT TCGAAATTCA CAGCCAGACG CAGACGACGG TGGCCAAGGG ATCGCCCGAG 
GGCGACGATG CGGAGCGAGA CAACCGAAAC TACGAGTATC TGACAATAGG GAAAGGCAAA 
ATTCGCCGGC GCTCAGACAA TGACGCTCAG ACAGATACTC TGCTCTTCAC CACTCGGGCA 
GTCAACACCA TCCTCATAAC CAAGGCCACC GTCAGCTCTT ACGTTTCCAA CTTTGAGATG 
TACGACACGC TGAACAATAT CACCAAGGAG TTCGCAACAG CCACTATGAG TTCGGTAAAG 
GACCTGACCT CGGCTCTAGC GCAGACGGAG GATGCGGATA CCTTTGAGGA CGACGAGGCC 
GTTGCCGAAA AAGACAAGGT CAGCAGCCAG ATAGAGCGCC TGTTCCGGAG CCCCGAGTTT 
CGCAACGCCA TCATGTACAT GGGCCGCACG CTCTCGAGCA ACACAAACGA GGCGGGGTTA 
CGACGCTTCC GTAACTTTGA CCAGGTGGAA CGCTGGAACG CGGAGGCCGA GTACGTCTAC 
TCGATGAACT TGCTCTTTCG GCTGGTACCG GAGCCCAGCA AAGACGAGCG CAAGGCCGTA 
AGCGACATTG ATTTCTGCCG CAGCAATGGT GACATCCTGG CTGTAGCCTA CGGACTGTAC 
TCATTTTCCT CACAGCACGT ACCGACCTCC GGCAGCGTTT TCCTGTGGAG CATTAAAAAT 
CCAGGCGAGC CGGAGCGCGC ATACTACTAC GATGTGCCAG TAACGGCAAT AAACTTCTCG 
CCATTCTTGC CATCCTTGCT CGCCATTGGT CTGTACGACG GCAGCGTGGA AGTGCGAGAT 
GTGAGCAGTT TGCAGGACAC ACCCGTTGCG GTCTCGCAGC GCAGCACTTC GCCAGGCTCC 
TCGCCGGTGG TTGCCATCCG CTGGATCAAA CAAGTTTCAG ACGACGATCA TGAAACAGAC 
ATCGATCCAT TCCTGAGCCT CTCCCAGGAT GGCTCGGTCA CCCGCTTCCG GATTATCAAA 
AGTCCCTTCC TGCTTGGTTT TACCCAAATG ATTCTGGAGC GAGTCGAGGG GCATCCTGAG 
GGCATTTTCG TTCACCCATC GTCGCGTATC CCCTGCGAGT CTAACCGCCA CCCGCAAGGC 
TTAAGCATCA CTACACATCC TCTGCACAAG GACATTTACT ACGTGCTGAC AGATGAGGGC 
TGCATCCACA AGTGCTCAAT CAATTATCAG CATCAGTACT TGGAAGTCCT GCGTTGTCAC 
GACGGGGGTG TCACCGTAAT GGAGTTCTCG CCTTGGTCTC CCAAACTCTT CCTCACCTGC 
GGCAACGATT GGTTCGTACG CATTTGGGTC GATGGCATCA CGCGACCTCT TCTCGAACTG 
CTAGACGAGA TGACCCCAGT GCATTGGGCC AAGTGGAGCC CCACCCACTC TACGATTATT 
GTGACGGTGA ACCGGGAAAC CGCTAACGTC TGGGACTTTC GCCGAAATTT GCTAAGACCC 
ATGTCCAAGC ACAAGATGGA CTCTTCTTTT AATACTGTGG CCAGGTTTTC CCACTGTGGT 
CGCACATTGG TTATTGGCAA CGAGCGCGGG AACACGCTCT TCAACGCCCT GAACGACATG 
CCCTTTTCGC CACACTTCCA ATACGACGAG TTAGAGAAAG CCATTTTCAA GGCAATTGGC 
AACGACCACG AGCTTCTTAT GGAGCTTAAA AGCATTGGTT TCTTTGGGTA CCCAAACAAA 
AAAGCAGTTT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728477.1
CDS	193..2487
Translation	MSASVRNTVTSQENGSIFKRSSNLDNELKKKSVVVNQTQAEESKAKKKDTTHYYSVETLADLQYRSQIKVLEKIYGEEVDMTPKSIQDDTYVEQFMKYNLNVMHQMTNFTTMVAKSTTRSRTRSSVRAGIGGNKQSIDDLMNGILYETINWEPYSTVEALEEAYEPKPYTKSFLKITLRKSDFFEIHSQTQTTVAKGSPEGDDAERDNRNYEYLTIGKGKIRRRSDNDAQTDTLLFTTRAVNTILITKATVSSYVSNFEMYDTLNNITKEFATATMSSVKDLTSALAQTEDADTFEDDEAVAEKDKVSSQIERLFRSPEFRNAIMYMGRTLSSNTNEAGLRRFRNFDQVERWNAEAEYVYSMNLLFRLVPEPSKDERKAVSDIDFCRSNGDILAVAYGLYSFSSQHVPTSGSVFLWSIKNPGEPERAYYYDVPVTAINFSPFLPSLLAIGLYDGSVEVRDVSSLQDTPVAVSQRSTSPGSSPVVAIRWIKQVSDDDHETDIDPFLSLSQDGSVTRFRIIKSPFLLGFTQMILERVEGHPEGIFVHPSSRIPCESNRHPQGLSITTHPLHKDIYYVLTDEGCIHKCSINYQHQYLEVLRCHDGGVTVMEFSPWSPKLFLTCGNDWFVRIWVDGITRPLLELLDEMTPVHWAKWSPTHSTIIVTVNRETANVWDFRRNLLRPMSKHKMDSSFNTVARFSHCGRTLVIGNERGNTLFNALNDMPFSPHFQYDELEKAIFKAIGNDHELLMELKSIGFFGYPNKKAVF

Target ORF information:

RefSeq Version	NM_167796.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dynein intermediate chain at 61B, transcript variant A (Dic61B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167796.3

ORF Insert Sequence:

ATGTCCGCGT CAGTAAGAAA TACAGTCACT AGCCAAGAAA ATGGGAGTAT CTTTAAACGG 
TCCAGTAACT TAGACAACGA ACTCAAAAAG AAGTCCGTGG TGGTTAATCA GACACAAGCC 
GAAGAGTCTA AGGCTAAGAA GAAAGATACT ACGCACTATT ACAGTGTGGA AACACTTGCC 
GACCTGCAGT ATCGATCTCA GATAAAGGTT TTGGAAAAGA TCTATGGCGA GGAGGTTGAT 
ATGACACCTA AGTCGATCCA AGATGACACC TATGTGGAGC AGTTCATGAA GTACAACCTA 
AATGTCATGC ACCAAATGAC TAACTTCACT ACAATGGTAG CGAAGAGCAC GACCAGATCC 
CGCACGCGGT CTAGTGTTCG GGCCGGAATA GGCGGCAATA AGCAGTCCAT TGACGACCTG 
ATGAACGGGA TACTTTATGA AACTATTAAC TGGGAACCGT ACAGCACTGT TGAGGCTCTC 
GAAGAGGCGT ACGAGCCAAA GCCGTATACC AAGTCCTTTT TAAAGATCAC CTTACGCAAG 
TCTGACTTCT TCGAAATTCA CAGCCAGACG CAGACGACGG TGGCCAAGGG ATCGCCCGAG 
GGCGACGATG CGGAGCGAGA CAACCGAAAC TACGAGTATC TGACAATAGG GAAAGGCAAA 
ATTCGCCGGC GCTCAGACAA TGACGCTCAG ACAGATACTC TGCTCTTCAC CACTCGGGCA 
GTCAACACCA TCCTCATAAC CAAGGCCACC GTCAGCTCTT ACGTTTCCAA CTTTGAGATG 
TACGACACGC TGAACAATAT CACCAAGGAG TTCGCAACAG CCACTATGAG TTCGGTAAAG 
GACCTGACCT CGGCTCTAGC GCAGACGGAG GATGCGGATA CCTTTGAGGA CGACGAGGCC 
GTTGCCGAAA AAGACAAGGT CAGCAGCCAG ATAGAGCGCC TGTTCCGGAG CCCCGAGTTT 
CGCAACGCCA TCATGTACAT GGGCCGCACG CTCTCGAGCA ACACAAACGA GGCGGGGTTA 
CGACGCTTCC GTAACTTTGA CCAGGTGGAA CGCTGGAACG CGGAGGCCGA GTACGTCTAC 
TCGATGAACT TGCTCTTTCG GCTGGTACCG GAGCCCAGCA AAGACGAGCG CAAGGCCGTA 
AGCGACATTG ATTTCTGCCG CAGCAATGGT GACATCCTGG CTGTAGCCTA CGGACTGTAC 
TCATTTTCCT CACAGCACGT ACCGACCTCC GGCAGCGTTT TCCTGTGGAG CATTAAAAAT 
CCAGGCGAGC CGGAGCGCGC ATACTACTAC GATGTGCCAG TAACGGCAAT AAACTTCTCG 
CCATTCTTGC CATCCTTGCT CGCCATTGGT CTGTACGACG GCAGCGTGGA AGTGCGAGAT 
GTGAGCAGTT TGCAGGACAC ACCCGTTGCG GTCTCGCAGC GCAGCACTTC GCCAGGCTCC 
TCGCCGGTGG TTGCCATCCG CTGGATCAAA CAAGTTTCAG ACGACGATCA TGAAACAGAC 
ATCGATCCAT TCCTGAGCCT CTCCCAGGAT GGCTCGGTCA CCCGCTTCCG GATTATCAAA 
AGTCCCTTCC TGCTTGGTTT TACCCAAATG ATTCTGGAGC GAGTCGAGGG GCATCCTGAG 
GGCATTTTCG TTCACCCATC GTCGCGTATC CCCTGCGAGT CTAACCGCCA CCCGCAAGGC 
TTAAGCATCA CTACACATCC TCTGCACAAG GACATTTACT ACGTGCTGAC AGATGAGGGC 
TGCATCCACA AGTGCTCAAT CAATTATCAG CATCAGTACT TGGAAGTCCT GCGTTGTCAC 
GACGGGGGTG TCACCGTAAT GGAGTTCTCG CCTTGGTCTC CCAAACTCTT CCTCACCTGC 
GGCAACGATT GGTTCGTACG CATTTGGGTC GATGGCATCA CGCGACCTCT TCTCGAACTG 
CTAGACGAGA TGACCCCAGT GCATTGGGCC AAGTGGAGCC CCACCCACTC TACGATTATT 
GTGACGGTGA ACCGGGAAAC CGCTAACGTC TGGGACTTTC GCCGAAATTT GCTAAGACCC 
ATGTCCAAGC ACAAGATGGA CTCTTCTTTT AATACTGTGG CCAGGTTTTC CCACTGTGGT 
CGCACATTGG TTATTGGCAA CGAGCGCGGG AACACGCTCT TCAACGCCCT GAACGACATG 
CCCTTTTCGC CACACTTCCA ATACGACGAG TTAGAGAAAG CCATTTTCAA GGCAATTGGC 
AACGACCACG AGCTTCTTAT GGAGCTTAAA AGCATTGGTT TCTTTGGGTA CCCAAACAAA 
AAAGCAGTTT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dic61B ( NM_138157.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_612001.1 Drosophila melanogaster dynein intermediate chain at 61B, transcript variant B (Dic61B), mRNA.

Price & Availability↑

CloneID	OFa17029
Clone ID Related Accession (Same CDS sequence)	NM_138157.3
Accession Version	NM_138157.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dynein intermediate chain at 61B, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Dec 17, 2009 this sequence version replaced NM_138157.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACACCTA AGTCGATCCA AGATGACACC TATGTGGAGC AGTTCATGAA GTACAACCTA 
AATGTCATGC ACCAAATGAC TAACTTCACT ACAATGGTAG CGAAGAGCAC GACCAGATCC 
CGCACGCGGT CTAGTGTTCG GGCCGGAATA GGCGGCAATA AGCAGTCCAT TGACGACCTG 
ATGAACGGGA TACTTTATGA AACTATTAAC TGGGAACCGT ACAGCACTGT TGAGGCTCTC 
GAAGAGGCGT ACGAGCCAAA GCCGTATACC AAGTCCTTTT TAAAGATCAC CTTACGCAAG 
TCTGACTTCT TCGAAATTCA CAGCCAGACG CAGACGACGG TGGCCAAGGG ATCGCCCGAG 
GGCGACGATG CGGAGCGAGA CAACCGAAAC TACGAGTATC TGACAATAGG GAAAGGCAAA 
ATTCGCCGGC GCTCAGACAA TGACGCTCAG ACAGATACTC TGCTCTTCAC CACTCGGGCA 
GTCAACACCA TCCTCATAAC CAAGGCCACC GTCAGCTCTT ACGTTTCCAA CTTTGAGATG 
TACGACACGC TGAACAATAT CACCAAGGAG TTCGCAACAG CCACTATGAG TTCGGTAAAG 
GACCTGACCT CGGCTCTAGC GCAGACGGAG GATGCGGATA CCTTTGAGGA CGACGAGGCC 
GTTGCCGAAA AAGACAAGGT CAGCAGCCAG ATAGAGCGCC TGTTCCGGAG CCCCGAGTTT 
CGCAACGCCA TCATGTACAT GGGCCGCACG CTCTCGAGCA ACACAAACGA GGCGGGGTTA 
CGACGCTTCC GTAACTTTGA CCAGGTGGAA CGCTGGAACG CGGAGGCCGA GTACGTCTAC 
TCGATGAACT TGCTCTTTCG GCTGGTACCG GAGCCCAGCA AAGACGAGCG CAAGGCCGTA 
AGCGACATTG ATTTCTGCCG CAGCAATGGT GACATCCTGG CTGTAGCCTA CGGACTGTAC 
TCATTTTCCT CACAGCACGT ACCGACCTCC GGCAGCGTTT TCCTGTGGAG CATTAAAAAT 
CCAGGCGAGC CGGAGCGCGC ATACTACTAC GATGTGCCAG TAACGGCAAT AAACTTCTCG 
CCATTCTTGC CATCCTTGCT CGCCATTGGT CTGTACGACG GCAGCGTGGA AGTGCGAGAT 
GTGAGCAGTT TGCAGGACAC ACCCGTTGCG GTCTCGCAGC GCAGCACTTC GCCAGGCTCC 
TCGCCGGTGG TTGCCATCCG CTGGATCAAA CAAGTTTCAG ACGACGATCA TGAAACAGAC 
ATCGATCCAT TCCTGAGCCT CTCCCAGGAT GGCTCGGTCA CCCGCTTCCG GATTATCAAA 
AGTCCCTTCC TGCTTGGTTT TACCCAAATG ATTCTGGAGC GAGTCGAGGG GCATCCTGAG 
GGCATTTTCG TTCACCCATC GTCGCGTATC CCCTGCGAGT CTAACCGCCA CCCGCAAGGC 
TTAAGCATCA CTACACATCC TCTGCACAAG GACATTTACT ACGTGCTGAC AGATGAGGGC 
TGCATCCACA AGTGCTCAAT CAATTATCAG CATCAGTACT TGGAAGTCCT GCGTTGTCAC 
GACGGGGGTG TCACCGTAAT GGAGTTCTCG CCTTGGTCTC CCAAACTCTT CCTCACCTGC 
GGCAACGATT GGTTCGTACG CATTTGGGTC GATGGCATCA CGCGACCTCT TCTCGAACTG 
CTAGACGAGA TGACCCCAGT GCATTGGGCC AAGTGGAGCC CCACCCACTC TACGATTATT 
GTGACGGTGA ACCGGGAAAC CGCTAACGTC TGGGACTTTC GCCGAAATTT GCTAAGACCC 
ATGTCCAAGC ACAAGATGGA CTCTTCTTTT AATACTGTGG CCAGGTTTTC CCACTGTGGT 
CGCACATTGG TTATTGGCAA CGAGCGCGGG AACACGCTCT TCAACGCCCT GAACGACATG 
CCCTTTTCGC CACACTTCCA ATACGACGAG TTAGAGAAAG CCATTTTCAA GGCAATTGGC 
AACGACCACG AGCTTCTTAT GGAGCTTAAA AGCATTGGTT TCTTTGGGTA CCCAAACAAA 
AAAGCAGTTT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_612001.1
CDS	459..2513
Translation	MTPKSIQDDTYVEQFMKYNLNVMHQMTNFTTMVAKSTTRSRTRSSVRAGIGGNKQSIDDLMNGILYETINWEPYSTVEALEEAYEPKPYTKSFLKITLRKSDFFEIHSQTQTTVAKGSPEGDDAERDNRNYEYLTIGKGKIRRRSDNDAQTDTLLFTTRAVNTILITKATVSSYVSNFEMYDTLNNITKEFATATMSSVKDLTSALAQTEDADTFEDDEAVAEKDKVSSQIERLFRSPEFRNAIMYMGRTLSSNTNEAGLRRFRNFDQVERWNAEAEYVYSMNLLFRLVPEPSKDERKAVSDIDFCRSNGDILAVAYGLYSFSSQHVPTSGSVFLWSIKNPGEPERAYYYDVPVTAINFSPFLPSLLAIGLYDGSVEVRDVSSLQDTPVAVSQRSTSPGSSPVVAIRWIKQVSDDDHETDIDPFLSLSQDGSVTRFRIIKSPFLLGFTQMILERVEGHPEGIFVHPSSRIPCESNRHPQGLSITTHPLHKDIYYVLTDEGCIHKCSINYQHQYLEVLRCHDGGVTVMEFSPWSPKLFLTCGNDWFVRIWVDGITRPLLELLDEMTPVHWAKWSPTHSTIIVTVNRETANVWDFRRNLLRPMSKHKMDSSFNTVARFSHCGRTLVIGNERGNTLFNALNDMPFSPHFQYDELEKAIFKAIGNDHELLMELKSIGFFGYPNKKAVF

Target ORF information:

RefSeq Version	NM_138157.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dynein intermediate chain at 61B, transcript variant B (Dic61B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138157.3

ORF Insert Sequence:

ATGACACCTA AGTCGATCCA AGATGACACC TATGTGGAGC AGTTCATGAA GTACAACCTA 
AATGTCATGC ACCAAATGAC TAACTTCACT ACAATGGTAG CGAAGAGCAC GACCAGATCC 
CGCACGCGGT CTAGTGTTCG GGCCGGAATA GGCGGCAATA AGCAGTCCAT TGACGACCTG 
ATGAACGGGA TACTTTATGA AACTATTAAC TGGGAACCGT ACAGCACTGT TGAGGCTCTC 
GAAGAGGCGT ACGAGCCAAA GCCGTATACC AAGTCCTTTT TAAAGATCAC CTTACGCAAG 
TCTGACTTCT TCGAAATTCA CAGCCAGACG CAGACGACGG TGGCCAAGGG ATCGCCCGAG 
GGCGACGATG CGGAGCGAGA CAACCGAAAC TACGAGTATC TGACAATAGG GAAAGGCAAA 
ATTCGCCGGC GCTCAGACAA TGACGCTCAG ACAGATACTC TGCTCTTCAC CACTCGGGCA 
GTCAACACCA TCCTCATAAC CAAGGCCACC GTCAGCTCTT ACGTTTCCAA CTTTGAGATG 
TACGACACGC TGAACAATAT CACCAAGGAG TTCGCAACAG CCACTATGAG TTCGGTAAAG 
GACCTGACCT CGGCTCTAGC GCAGACGGAG GATGCGGATA CCTTTGAGGA CGACGAGGCC 
GTTGCCGAAA AAGACAAGGT CAGCAGCCAG ATAGAGCGCC TGTTCCGGAG CCCCGAGTTT 
CGCAACGCCA TCATGTACAT GGGCCGCACG CTCTCGAGCA ACACAAACGA GGCGGGGTTA 
CGACGCTTCC GTAACTTTGA CCAGGTGGAA CGCTGGAACG CGGAGGCCGA GTACGTCTAC 
TCGATGAACT TGCTCTTTCG GCTGGTACCG GAGCCCAGCA AAGACGAGCG CAAGGCCGTA 
AGCGACATTG ATTTCTGCCG CAGCAATGGT GACATCCTGG CTGTAGCCTA CGGACTGTAC 
TCATTTTCCT CACAGCACGT ACCGACCTCC GGCAGCGTTT TCCTGTGGAG CATTAAAAAT 
CCAGGCGAGC CGGAGCGCGC ATACTACTAC GATGTGCCAG TAACGGCAAT AAACTTCTCG 
CCATTCTTGC CATCCTTGCT CGCCATTGGT CTGTACGACG GCAGCGTGGA AGTGCGAGAT 
GTGAGCAGTT TGCAGGACAC ACCCGTTGCG GTCTCGCAGC GCAGCACTTC GCCAGGCTCC 
TCGCCGGTGG TTGCCATCCG CTGGATCAAA CAAGTTTCAG ACGACGATCA TGAAACAGAC 
ATCGATCCAT TCCTGAGCCT CTCCCAGGAT GGCTCGGTCA CCCGCTTCCG GATTATCAAA 
AGTCCCTTCC TGCTTGGTTT TACCCAAATG ATTCTGGAGC GAGTCGAGGG GCATCCTGAG 
GGCATTTTCG TTCACCCATC GTCGCGTATC CCCTGCGAGT CTAACCGCCA CCCGCAAGGC 
TTAAGCATCA CTACACATCC TCTGCACAAG GACATTTACT ACGTGCTGAC AGATGAGGGC 
TGCATCCACA AGTGCTCAAT CAATTATCAG CATCAGTACT TGGAAGTCCT GCGTTGTCAC 
GACGGGGGTG TCACCGTAAT GGAGTTCTCG CCTTGGTCTC CCAAACTCTT CCTCACCTGC 
GGCAACGATT GGTTCGTACG CATTTGGGTC GATGGCATCA CGCGACCTCT TCTCGAACTG 
CTAGACGAGA TGACCCCAGT GCATTGGGCC AAGTGGAGCC CCACCCACTC TACGATTATT 
GTGACGGTGA ACCGGGAAAC CGCTAACGTC TGGGACTTTC GCCGAAATTT GCTAAGACCC 
ATGTCCAAGC ACAAGATGGA CTCTTCTTTT AATACTGTGG CCAGGTTTTC CCACTGTGGT 
CGCACATTGG TTATTGGCAA CGAGCGCGGG AACACGCTCT TCAACGCCCT GAACGACATG 
CCCTTTTCGC CACACTTCCA ATACGACGAG TTAGAGAAAG CCATTTTCAA GGCAATTGGC 
AACGACCACG AGCTTCTTAT GGAGCTTAAA AGCATTGGTT TCTTTGGGTA CCCAAACAAA 
AAAGCAGTTT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
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Dic61B cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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