pain cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	pain
Entrez Gene ID	37985
Full Name	painless
Synonyms	CG15860,CT31987,Dmel\CG15860,EP2251,Pain
General protein information	Preferred Names painless Names CG15860 gene product from transcript CG15860-RB CG15860-PA CG15860-PB CG15860-PC pain-PA pain-PB pain-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 60E5-60E5

mRNA and Protein(s)

mRNA	Protein	Name
NM_138135.2	NP_611979.1	painless, isoform A
NM_001274248.1	NP_001261177.1	painless, isoform C
NM_001274247.2	NP_001261176.1	painless, isoform B

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP013463	XP_003436057.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012995	XP_003436056.1
Drosophila melanogaster (fruit fly)	pain	NP_611979.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP013366	XP_003436055.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our findings show that inactivation of these sensory neurons impairs the cellular response against wasp parasitization. We also demonstrate that the nociception genes encoding the mechanosensory receptors Painless and Piezo, both expressed in class IV neurons, are essential for the normal cellular immune response to parasite challenge.
Title: The nociception genes painless and Piezo are required for the cellular immune response of Drosophila larvae to wasp parasitization.

Recent work has uncovered the painless, mediating fly thermosensation.
Title: Temperature sensation in Drosophila.

For the regulation of sexual receptivity through Pain TRP channels.
Title: Insulin-producing cells regulate the sexual receptivity through the painless TRP channel in Drosophila virgin females.

class IV multiple-dendritic(mdIV) nociceptors are also required for a normal larval aversion to locomotion on to a dry surface environment utilizing painless and PPK1
Title: Drosophila nociceptors mediate larval aversion to dry surface environments utilizing both the painless TRP channel and the DEG/ENaC subunit, PPK1.

in the case of Painless, ankryin repeats are important for thermal nociception but not for mechanical nociception
Title: The ankyrin repeat domain of the TRPA protein painless is important for thermal nociception but not mechanical nociception.

The following pain gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pain cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13261	NM_001274247.2 Latest version!	Drosophila melanogaster painless (pain), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa13262	NM_001274248.1 Latest version!	Drosophila melanogaster painless (pain), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa11257	NM_138135.2 Latest version!	Drosophila melanogaster painless (pain), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pain ( NM_001274247.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261176.1 Drosophila melanogaster painless (pain), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa13261
Clone ID Related Accession (Same CDS sequence)	NM_001274247.2
Accession Version	NM_001274247.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	painless, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001274247.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATATCA ACTCGAGACC AGGCAGGGCC AACGAGGCCA ATCTCGTAGA GACGGCCGTG 
ATATACGGTA ACTGGCAGGC GTTGGAGCGA CTGCTTAAGG AGCCAAACCT GCGACTTACT 
CCAGACTCCA AGCTACTAAA TGCAGTAATC GGCCGTCTGG ATGAGCCACC GTATGATGGC 
TCCAGCCACC AGCGCTGCTT TGAATTGCTC ATTAACAGCG ATCGCGTAGA CATCAACGAA 
GCTGATTCCG GACGCCTGGT GCCTCTGTTC TTCGCTGTTA AGTACCGCAA CACGAGTGCG 
ATGCAAAAAC TCCTGAAGAA CGGTGCCTAC ATTGGTTCTA AGAGCGCATT TGGCACACTA 
CCCATCAAGG ACATGCCACC CGAGGTTCTC GAAGAGCACT TCGACTCGTG TATCACCACA 
AACGGAGAGA GGCCTGGTGA CCAGAACTTT GAGATCATCA TCGATTATAA GAACCTAATG 
CGCCAGGAGA GAGACTCCGG ACTCAACCAG CTGCAAGACG AAATGGCCCC GATCGCATTC 
ATCGCCGAGT CGAAGGAGAT GCGCCACCTG CTCCAGCACC CGCTGATCTC GAGCTTTCTA 
TTCCTCAAGT GGCACCGACT TTCCGTGATA TTCTACCTGA ACTTCCTGAT ATACTCGCTT 
TTTACCGCCT CCATAATTAC CTACACGCTC CTCAAGTTCC ACGAAAGCGA TCAAAGGGCT 
CTTACTGCAT TTTTCGGATT GCTTTCCTGG CTGGGAATCA GCTACCTTAT ATTACGGGAG 
TGCATCCAGT GGATAATGTC TCCAGTTCGG TACTTTTGGT CTATAACGAA TATTATGGAG 
GTGGCTCTTA TTACACTATC TATCTTTACC TGCATGGAAT CCAGCTTCGA CAAGGAGACG 
CAGCGCGTCT TAGCCGTATT TACCATCCTA CTCGTCTCCA TGGAGTTTTG TTTACTAGTG 
GGCTCCCTGC CAGTGCTCTC AATTTCGACG CACATGCTGA TGCTGCGAGA GGTGTCAAAC 
AGCTTCTTAA AGAGCTTTAC CCTCTACTCG ATCTTCGTGC TCACCTTCAG CCTGTGTTTC 
TATATCCTCT TCGGCAAGTC AGTGGAGGAA GACCAGTCTA AAAGCGCTAC GCCATGTCCA 
CCTCTGGGGA AGAAGGAGGG GAAGGACGAG GAACAGGGCT TCAACACATT TACCAAGCCT 
ATCGAGGCCG TGATCAAGAC CATTGTGATG CTGACAGGCG AGTTTGACGC CGGAAGCATC 
CAGTTTACCA GCATCTACAC CTACCTGATT TTCCTGCTCT TCGTGATCTT TATGACGATA 
GTGCTGTTCA ACCTTTTGAA CGGTCTTGCA GTGAGCGACA CCCAAGTTAT TAAGGCTCAG 
GCGGAACTGA ACGGAGCCAT TTGCAGAACC AACGTCCTTA GTCGGTACGA GCAGGTTCTC 
ACTGGCCACG GACGCGCTGG GTTTTTGTTG GGCAACCATC TCTTCCGCAG CATCTGCCAA 
CGTTTGATGA ACATCTACCC GAACTACTTA AGTCTGCGTC AGATTTCCGT GCTGCCGAAC 
GATGGAAACA AAGTGCTTAT TCCAATGAGC GATCCCTTCG AAATGAGGAC CCTTAAGAAG 
GCTAGCTTTC AGCAATTGCC CCTGAGTGCT GCAGTGCCCC AGAAGAAGCT GTTGGATCCA 
CCGCTTAGAC TTCTGCCCTG CTGCTGTTCC CTGCTCACCG GAAAGTGCTC CCAGATGAGC 
GGCCGGGTGG TCAAACGGGC CCTCGAGGTA ATCGATCAGA AGAACGCGGC GGAGCAGAGG 
CGGAAACAGG AACAGATCAA CGACAGTCGA CTGAAGCTGA TCGAGTACAA GCTGGAGCAA 
TTAATACAGC TGGTCCAGGA CCGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261176.1
CDS	221..2110
Translation	MDINSRPGRANEANLVETAVIYGNWQALERLLKEPNLRLTPDSKLLNAVIGRLDEPPYDGSSHQRCFELLINSDRVDINEADSGRLVPLFFAVKYRNTSAMQKLLKNGAYIGSKSAFGTLPIKDMPPEVLEEHFDSCITTNGERPGDQNFEIIIDYKNLMRQERDSGLNQLQDEMAPIAFIAESKEMRHLLQHPLISSFLFLKWHRLSVIFYLNFLIYSLFTASIITYTLLKFHESDQRALTAFFGLLSWLGISYLILRECIQWIMSPVRYFWSITNIMEVALITLSIFTCMESSFDKETQRVLAVFTILLVSMEFCLLVGSLPVLSISTHMLMLREVSNSFLKSFTLYSIFVLTFSLCFYILFGKSVEEDQSKSATPCPPLGKKEGKDEEQGFNTFTKPIEAVIKTIVMLTGEFDAGSIQFTSIYTYLIFLLFVIFMTIVLFNLLNGLAVSDTQVIKAQAELNGAICRTNVLSRYEQVLTGHGRAGFLLGNHLFRSICQRLMNIYPNYLSLRQISVLPNDGNKVLIPMSDPFEMRTLKKASFQQLPLSAAVPQKKLLDPPLRLLPCCCSLLTGKCSQMSGRVVKRALEVIDQKNAAEQRRKQEQINDSRLKLIEYKLEQLIQLVQDRK

Target ORF information:

RefSeq Version	NM_001274247.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster painless (pain), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274247.2

ORF Insert Sequence:

ATGGATATCA ACTCGAGACC AGGCAGGGCC AACGAGGCCA ATCTCGTAGA GACGGCCGTG 
ATATACGGTA ACTGGCAGGC GTTGGAGCGA CTGCTTAAGG AGCCAAACCT GCGACTTACT 
CCAGACTCCA AGCTACTAAA TGCAGTAATC GGCCGTCTGG ATGAGCCACC GTATGATGGC 
TCCAGCCACC AGCGCTGCTT TGAATTGCTC ATTAACAGCG ATCGCGTAGA CATCAACGAA 
GCTGATTCCG GACGCCTGGT GCCTCTGTTC TTCGCTGTTA AGTACCGCAA CACGAGTGCG 
ATGCAAAAAC TCCTGAAGAA CGGTGCCTAC ATTGGTTCTA AGAGCGCATT TGGCACACTA 
CCCATCAAGG ACATGCCACC CGAGGTTCTC GAAGAGCACT TCGACTCGTG TATCACCACA 
AACGGAGAGA GGCCTGGTGA CCAGAACTTT GAGATCATCA TCGATTATAA GAACCTAATG 
CGCCAGGAGA GAGACTCCGG ACTCAACCAG CTGCAAGACG AAATGGCCCC GATCGCATTC 
ATCGCCGAGT CGAAGGAGAT GCGCCACCTG CTCCAGCACC CGCTGATCTC GAGCTTTCTA 
TTCCTCAAGT GGCACCGACT TTCCGTGATA TTCTACCTGA ACTTCCTGAT ATACTCGCTT 
TTTACCGCCT CCATAATTAC CTACACGCTC CTCAAGTTCC ACGAAAGCGA TCAAAGGGCT 
CTTACTGCAT TTTTCGGATT GCTTTCCTGG CTGGGAATCA GCTACCTTAT ATTACGGGAG 
TGCATCCAGT GGATAATGTC TCCAGTTCGG TACTTTTGGT CTATAACGAA TATTATGGAG 
GTGGCTCTTA TTACACTATC TATCTTTACC TGCATGGAAT CCAGCTTCGA CAAGGAGACG 
CAGCGCGTCT TAGCCGTATT TACCATCCTA CTCGTCTCCA TGGAGTTTTG TTTACTAGTG 
GGCTCCCTGC CAGTGCTCTC AATTTCGACG CACATGCTGA TGCTGCGAGA GGTGTCAAAC 
AGCTTCTTAA AGAGCTTTAC CCTCTACTCG ATCTTCGTGC TCACCTTCAG CCTGTGTTTC 
TATATCCTCT TCGGCAAGTC AGTGGAGGAA GACCAGTCTA AAAGCGCTAC GCCATGTCCA 
CCTCTGGGGA AGAAGGAGGG GAAGGACGAG GAACAGGGCT TCAACACATT TACCAAGCCT 
ATCGAGGCCG TGATCAAGAC CATTGTGATG CTGACAGGCG AGTTTGACGC CGGAAGCATC 
CAGTTTACCA GCATCTACAC CTACCTGATT TTCCTGCTCT TCGTGATCTT TATGACGATA 
GTGCTGTTCA ACCTTTTGAA CGGTCTTGCA GTGAGCGACA CCCAAGTTAT TAAGGCTCAG 
GCGGAACTGA ACGGAGCCAT TTGCAGAACC AACGTCCTTA GTCGGTACGA GCAGGTTCTC 
ACTGGCCACG GACGCGCTGG GTTTTTGTTG GGCAACCATC TCTTCCGCAG CATCTGCCAA 
CGTTTGATGA ACATCTACCC GAACTACTTA AGTCTGCGTC AGATTTCCGT GCTGCCGAAC 
GATGGAAACA AAGTGCTTAT TCCAATGAGC GATCCCTTCG AAATGAGGAC CCTTAAGAAG 
GCTAGCTTTC AGCAATTGCC CCTGAGTGCT GCAGTGCCCC AGAAGAAGCT GTTGGATCCA 
CCGCTTAGAC TTCTGCCCTG CTGCTGTTCC CTGCTCACCG GAAAGTGCTC CCAGATGAGC 
GGCCGGGTGG TCAAACGGGC CCTCGAGGTA ATCGATCAGA AGAACGCGGC GGAGCAGAGG 
CGGAAACAGG AACAGATCAA CGACAGTCGA CTGAAGCTGA TCGAGTACAA GCTGGAGCAA 
TTAATACAGC TGGTCCAGGA CCGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pain ( NM_001274248.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261177.1 Drosophila melanogaster painless (pain), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa13262
Clone ID Related Accession (Same CDS sequence)	NM_001274248.1
Accession Version	NM_001274248.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	painless, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAAAAAC TCCTGAAGAA CGGTGCCTAC ATTGGTTCTA AGAGCGCATT TGGCACACTA 
CCCATCAAGG ACATGCCACC CGAGGTTCTC GAAGAGCACT TCGACTCGTG TATCACCACA 
AACGGAGAGA GGCCTGGTGA CCAGAACTTT GAGATCATCA TCGATTATAA GAACCTAATG 
CGCCAGGAGA GAGACTCCGG ACTCAACCAG CTGCAAGACG AAATGGCCCC GATCGCATTC 
ATCGCCGAGT CGAAGGAGAT GCGCCACCTG CTCCAGCACC CGCTGATCTC GAGCTTTCTA 
TTCCTCAAGT GGCACCGACT TTCCGTGATA TTCTACCTGA ACTTCCTGAT ATACTCGCTT 
TTTACCGCCT CCATAATTAC CTACACGCTC CTCAAGTTCC ACGAAAGCGA TCAAAGGGCT 
CTTACTGCAT TTTTCGGATT GCTTTCCTGG CTGGGAATCA GCTACCTTAT ATTACGGGAG 
TGCATCCAGT GGATAATGTC TCCAGTTCGG TACTTTTGGT CTATAACGAA TATTATGGAG 
GTGGCTCTTA TTACACTATC TATCTTTACC TGCATGGAAT CCAGCTTCGA CAAGGAGACG 
CAGCGCGTCT TAGCCGTATT TACCATCCTA CTCGTCTCCA TGGAGTTTTG TTTACTAGTG 
GGCTCCCTGC CAGTGCTCTC AATTTCGACG CACATGCTGA TGCTGCGAGA GGTGTCAAAC 
AGCTTCTTAA AGAGCTTTAC CCTCTACTCG ATCTTCGTGC TCACCTTCAG CCTGTGTTTC 
TATATCCTCT TCGGCAAGTC AGTGGAGGAA GACCAGTCTA AAAGCGCTAC GCCATGTCCA 
CCTCTGGGGA AGAAGGAGGG GAAGGACGAG GAACAGGGCT TCAACACATT TACCAAGCCT 
ATCGAGGCCG TGATCAAGAC CATTGTGATG CTGACAGGCG AGTTTGACGC CGGAAGCATC 
CAGTTTACCA GCATCTACAC CTACCTGATT TTCCTGCTCT TCGTGATCTT TATGACGATA 
GTGCTGTTCA ACCTTTTGAA CGGTCTTGCA GTGAGCGACA CCCAAGTTAT TAAGGCTCAG 
GCGGAACTGA ACGGAGCCAT TTGCAGAACC AACGTCCTTA GTCGGTACGA GCAGGTTCTC 
ACTGGCCACG GACGCGCTGG GTTTTTGTTG GGCAACCATC TCTTCCGCAG CATCTGCCAA 
CGTTTGATGA ACATCTACCC GAACTACTTA AGTCTGCGTC AGATTTCCGT GCTGCCGAAC 
GATGGAAACA AAGTGCTTAT TCCAATGAGC GATCCCTTCG AAATGAGGAC CCTTAAGAAG 
GCTAGCTTTC AGCAATTGCC CCTGAGTGCT GCAGTGCCCC AGAAGAAGCT GTTGGATCCA 
CCGCTTAGAC TTCTGCCCTG CTGCTGTTCC CTGCTCACCG GAAAGTGCTC CCAGATGAGC 
GGCCGGGTGG TCAAACGGGC CCTCGAGGTA ATCGATCAGA AGAACGCGGC GGAGCAGAGG 
CGGAAACAGG AACAGATCAA CGACAGTCGA CTGAAGCTGA TCGAGTACAA GCTGGAGCAA 
TTAATACAGC TGGTCCAGGA CCGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261177.1
CDS	177..1766
Translation	MQKLLKNGAYIGSKSAFGTLPIKDMPPEVLEEHFDSCITTNGERPGDQNFEIIIDYKNLMRQERDSGLNQLQDEMAPIAFIAESKEMRHLLQHPLISSFLFLKWHRLSVIFYLNFLIYSLFTASIITYTLLKFHESDQRALTAFFGLLSWLGISYLILRECIQWIMSPVRYFWSITNIMEVALITLSIFTCMESSFDKETQRVLAVFTILLVSMEFCLLVGSLPVLSISTHMLMLREVSNSFLKSFTLYSIFVLTFSLCFYILFGKSVEEDQSKSATPCPPLGKKEGKDEEQGFNTFTKPIEAVIKTIVMLTGEFDAGSIQFTSIYTYLIFLLFVIFMTIVLFNLLNGLAVSDTQVIKAQAELNGAICRTNVLSRYEQVLTGHGRAGFLLGNHLFRSICQRLMNIYPNYLSLRQISVLPNDGNKVLIPMSDPFEMRTLKKASFQQLPLSAAVPQKKLLDPPLRLLPCCCSLLTGKCSQMSGRVVKRALEVIDQKNAAEQRRKQEQINDSRLKLIEYKLEQLIQLVQDRK

Target ORF information:

RefSeq Version	NM_001274248.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster painless (pain), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274248.1

ORF Insert Sequence:

ATGCAAAAAC TCCTGAAGAA CGGTGCCTAC ATTGGTTCTA AGAGCGCATT TGGCACACTA 
CCCATCAAGG ACATGCCACC CGAGGTTCTC GAAGAGCACT TCGACTCGTG TATCACCACA 
AACGGAGAGA GGCCTGGTGA CCAGAACTTT GAGATCATCA TCGATTATAA GAACCTAATG 
CGCCAGGAGA GAGACTCCGG ACTCAACCAG CTGCAAGACG AAATGGCCCC GATCGCATTC 
ATCGCCGAGT CGAAGGAGAT GCGCCACCTG CTCCAGCACC CGCTGATCTC GAGCTTTCTA 
TTCCTCAAGT GGCACCGACT TTCCGTGATA TTCTACCTGA ACTTCCTGAT ATACTCGCTT 
TTTACCGCCT CCATAATTAC CTACACGCTC CTCAAGTTCC ACGAAAGCGA TCAAAGGGCT 
CTTACTGCAT TTTTCGGATT GCTTTCCTGG CTGGGAATCA GCTACCTTAT ATTACGGGAG 
TGCATCCAGT GGATAATGTC TCCAGTTCGG TACTTTTGGT CTATAACGAA TATTATGGAG 
GTGGCTCTTA TTACACTATC TATCTTTACC TGCATGGAAT CCAGCTTCGA CAAGGAGACG 
CAGCGCGTCT TAGCCGTATT TACCATCCTA CTCGTCTCCA TGGAGTTTTG TTTACTAGTG 
GGCTCCCTGC CAGTGCTCTC AATTTCGACG CACATGCTGA TGCTGCGAGA GGTGTCAAAC 
AGCTTCTTAA AGAGCTTTAC CCTCTACTCG ATCTTCGTGC TCACCTTCAG CCTGTGTTTC 
TATATCCTCT TCGGCAAGTC AGTGGAGGAA GACCAGTCTA AAAGCGCTAC GCCATGTCCA 
CCTCTGGGGA AGAAGGAGGG GAAGGACGAG GAACAGGGCT TCAACACATT TACCAAGCCT 
ATCGAGGCCG TGATCAAGAC CATTGTGATG CTGACAGGCG AGTTTGACGC CGGAAGCATC 
CAGTTTACCA GCATCTACAC CTACCTGATT TTCCTGCTCT TCGTGATCTT TATGACGATA 
GTGCTGTTCA ACCTTTTGAA CGGTCTTGCA GTGAGCGACA CCCAAGTTAT TAAGGCTCAG 
GCGGAACTGA ACGGAGCCAT TTGCAGAACC AACGTCCTTA GTCGGTACGA GCAGGTTCTC 
ACTGGCCACG GACGCGCTGG GTTTTTGTTG GGCAACCATC TCTTCCGCAG CATCTGCCAA 
CGTTTGATGA ACATCTACCC GAACTACTTA AGTCTGCGTC AGATTTCCGT GCTGCCGAAC 
GATGGAAACA AAGTGCTTAT TCCAATGAGC GATCCCTTCG AAATGAGGAC CCTTAAGAAG 
GCTAGCTTTC AGCAATTGCC CCTGAGTGCT GCAGTGCCCC AGAAGAAGCT GTTGGATCCA 
CCGCTTAGAC TTCTGCCCTG CTGCTGTTCC CTGCTCACCG GAAAGTGCTC CCAGATGAGC 
GGCCGGGTGG TCAAACGGGC CCTCGAGGTA ATCGATCAGA AGAACGCGGC GGAGCAGAGG 
CGGAAACAGG AACAGATCAA CGACAGTCGA CTGAAGCTGA TCGAGTACAA GCTGGAGCAA 
TTAATACAGC TGGTCCAGGA CCGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pain ( NM_138135.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611979.1 Drosophila melanogaster painless (pain), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa11257
Clone ID Related Accession (Same CDS sequence)	NM_138135.2
Accession Version	NM_138135.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	painless, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_138135.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACTTTA ACAACTGCGG CTTCATTGAT CCGCAGGCCC AGCTAGCTGG AGCTTTGGCC 
AAGCAGGACA TCCGACAGTT CGTTGCTGCC CTGGACAGCG GTGCCCTGGC CGATCTACAA 
GACGACCGCC ATACCAGTAT CTACGAGAAG GCACTCTCAA CACCAGGTTG TCGTGACTTC 
ATTGAAGCCT GCATCGACCA CGGCAGCCAG GTGAACTACA TCAACAAGAA GCTGGACAAG 
GCCGCAATCA GCTATGCGGC TGACTCTAGG GATCCAGGAA ACCTGGCGGC TCTCCTTAAG 
TACCGCCCCG GAAACAAAGT CCAGGTTGAT AGAAAATATG GGCAGCTTAC TCCACTTAAC 
TCACTTGCCA AGAATCTCAC GGATGAAAAT GCCCCAGACG TGTACTCCTG CATGCAACTC 
TTGCTGGACT ACGGCGCCTC GCCGAATATC GTAGACCAGG GCGAGTTCAC ACCCTTGCAC 
CATGTGCTGA GAAAGAGCAA GGTGAAGGCT GGGAAGAAGG AACTGATTCA GCTCTTTCTG 
GACCATCCGG AGCTGGATAT CGATAGTTAC CGAAACGGGG AGGTGCGCAG ACTGCTGCAG 
GCGCAATTTC CGGAGCTTAA GCTGCCGGAA GAGCGTCATA CCGGGCCGGA GATTGACATC 
CAAACTCTTC AAAGGACTCT ACGGGACGGG GACGAAACAC TGTTTGAGCA GCAGTTCGCT 
GAGTACTTGC AGAATCTCAA AGGCGGAGCG GATAACCAAC TAAATGCCCA CCAGGAGGAA 
TACTTCGGAC TGCTGCAGGA GAGCATCAAG AGGGGCAGGC AGCGAGCCTT CGATGTCATT 
TTGTCCACTG GCATGGATAT CAACTCGAGA CCAGGCAGGG CCAACGAGGC CAATCTCGTA 
GAGACGGCCG TGATATACGG TAACTGGCAG GCGTTGGAGC GACTGCTTAA GGAGCCAAAC 
CTGCGACTTA CTCCAGACTC CAAGCTACTA AATGCAGTAA TCGGCCGTCT GGATGAGCCA 
CCGTATGATG GCTCCAGCCA CCAGCGCTGC TTTGAATTGC TCATTAACAG CGATCGCGTA 
GACATCAACG AAGCTGATTC CGGACGCCTG GTGCCTCTGT TCTTCGCTGT TAAGTACCGC 
AACACGAGTG CGATGCAAAA ACTCCTGAAG AACGGTGCCT ACATTGGTTC TAAGAGCGCA 
TTTGGCACAC TACCCATCAA GGACATGCCA CCCGAGGTTC TCGAAGAGCA CTTCGACTCG 
TGTATCACCA CAAACGGAGA GAGGCCTGGT GACCAGAACT TTGAGATCAT CATCGATTAT 
AAGAACCTAA TGCGCCAGGA GAGAGACTCC GGACTCAACC AGCTGCAAGA CGAAATGGCC 
CCGATCGCAT TCATCGCCGA GTCGAAGGAG ATGCGCCACC TGCTCCAGCA CCCGCTGATC 
TCGAGCTTTC TATTCCTCAA GTGGCACCGA CTTTCCGTGA TATTCTACCT GAACTTCCTG 
ATATACTCGC TTTTTACCGC CTCCATAATT ACCTACACGC TCCTCAAGTT CCACGAAAGC 
GATCAAAGGG CTCTTACTGC ATTTTTCGGA TTGCTTTCCT GGCTGGGAAT CAGCTACCTT 
ATATTACGGG AGTGCATCCA GTGGATAATG TCTCCAGTTC GGTACTTTTG GTCTATAACG 
AATATTATGG AGGTGGCTCT TATTACACTA TCTATCTTTA CCTGCATGGA ATCCAGCTTC 
GACAAGGAGA CGCAGCGCGT CTTAGCCGTA TTTACCATCC TACTCGTCTC CATGGAGTTT 
TGTTTACTAG TGGGCTCCCT GCCAGTGCTC TCAATTTCGA CGCACATGCT GATGCTGCGA 
GAGGTGTCAA ACAGCTTCTT AAAGAGCTTT ACCCTCTACT CGATCTTCGT GCTCACCTTC 
AGCCTGTGTT TCTATATCCT CTTCGGCAAG TCAGTGGAGG AAGACCAGTC TAAAAGCGCT 
ACGCCATGTC CACCTCTGGG GAAGAAGGAG GGGAAGGACG AGGAACAGGG CTTCAACACA 
TTTACCAAGC CTATCGAGGC CGTGATCAAG ACCATTGTGA TGCTGACAGG CGAGTTTGAC 
GCCGGAAGCA TCCAGTTTAC CAGCATCTAC ACCTACCTGA TTTTCCTGCT CTTCGTGATC 
TTTATGACGA TAGTGCTGTT CAACCTTTTG AACGGTCTTG CAGTGAGCGA CACCCAAGTT 
ATTAAGGCTC AGGCGGAACT GAACGGAGCC ATTTGCAGAA CCAACGTCCT TAGTCGGTAC 
GAGCAGGTTC TCACTGGCCA CGGACGCGCT GGGTTTTTGT TGGGCAACCA TCTCTTCCGC 
AGCATCTGCC AACGTTTGAT GAACATCTAC CCGAACTACT TAAGTCTGCG TCAGATTTCC 
GTGCTGCCGA ACGATGGAAA CAAAGTGCTT ATTCCAATGA GCGATCCCTT CGAAATGAGG 
ACCCTTAAGA AGGCTAGCTT TCAGCAATTG CCCCTGAGTG CTGCAGTGCC CCAGAAGAAG 
CTGTTGGATC CACCGCTTAG ACTTCTGCCC TGCTGCTGTT CCCTGCTCAC CGGAAAGTGC 
TCCCAGATGA GCGGCCGGGT GGTCAAACGG GCCCTCGAGG TAATCGATCA GAAGAACGCG 
GCGGAGCAGA GGCGGAAACA GGAACAGATC AACGACAGTC GACTGAAGCT GATCGAGTAC 
AAGCTGGAGC AATTAATACA GCTGGTCCAG GACCGGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611979.1
CDS	41..2782
Translation	MDFNNCGFIDPQAQLAGALAKQDIRQFVAALDSGALADLQDDRHTSIYEKALSTPGCRDFIEACIDHGSQVNYINKKLDKAAISYAADSRDPGNLAALLKYRPGNKVQVDRKYGQLTPLNSLAKNLTDENAPDVYSCMQLLLDYGASPNIVDQGEFTPLHHVLRKSKVKAGKKELIQLFLDHPELDIDSYRNGEVRRLLQAQFPELKLPEERHTGPEIDIQTLQRTLRDGDETLFEQQFAEYLQNLKGGADNQLNAHQEEYFGLLQESIKRGRQRAFDVILSTGMDINSRPGRANEANLVETAVIYGNWQALERLLKEPNLRLTPDSKLLNAVIGRLDEPPYDGSSHQRCFELLINSDRVDINEADSGRLVPLFFAVKYRNTSAMQKLLKNGAYIGSKSAFGTLPIKDMPPEVLEEHFDSCITTNGERPGDQNFEIIIDYKNLMRQERDSGLNQLQDEMAPIAFIAESKEMRHLLQHPLISSFLFLKWHRLSVIFYLNFLIYSLFTASIITYTLLKFHESDQRALTAFFGLLSWLGISYLILRECIQWIMSPVRYFWSITNIMEVALITLSIFTCMESSFDKETQRVLAVFTILLVSMEFCLLVGSLPVLSISTHMLMLREVSNSFLKSFTLYSIFVLTFSLCFYILFGKSVEEDQSKSATPCPPLGKKEGKDEEQGFNTFTKPIEAVIKTIVMLTGEFDAGSIQFTSIYTYLIFLLFVIFMTIVLFNLLNGLAVSDTQVIKAQAELNGAICRTNVLSRYEQVLTGHGRAGFLLGNHLFRSICQRLMNIYPNYLSLRQISVLPNDGNKVLIPMSDPFEMRTLKKASFQQLPLSAAVPQKKLLDPPLRLLPCCCSLLTGKCSQMSGRVVKRALEVIDQKNAAEQRRKQEQINDSRLKLIEYKLEQLIQLVQDRK

Target ORF information:

RefSeq Version	NM_138135.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster painless (pain), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138135.2

ORF Insert Sequence:

ATGGACTTTA ACAACTGCGG CTTCATTGAT CCGCAGGCCC AGCTAGCTGG AGCTTTGGCC 
AAGCAGGACA TCCGACAGTT CGTTGCTGCC CTGGACAGCG GTGCCCTGGC CGATCTACAA 
GACGACCGCC ATACCAGTAT CTACGAGAAG GCACTCTCAA CACCAGGTTG TCGTGACTTC 
ATTGAAGCCT GCATCGACCA CGGCAGCCAG GTGAACTACA TCAACAAGAA GCTGGACAAG 
GCCGCAATCA GCTATGCGGC TGACTCTAGG GATCCAGGAA ACCTGGCGGC TCTCCTTAAG 
TACCGCCCCG GAAACAAAGT CCAGGTTGAT AGAAAATATG GGCAGCTTAC TCCACTTAAC 
TCACTTGCCA AGAATCTCAC GGATGAAAAT GCCCCAGACG TGTACTCCTG CATGCAACTC 
TTGCTGGACT ACGGCGCCTC GCCGAATATC GTAGACCAGG GCGAGTTCAC ACCCTTGCAC 
CATGTGCTGA GAAAGAGCAA GGTGAAGGCT GGGAAGAAGG AACTGATTCA GCTCTTTCTG 
GACCATCCGG AGCTGGATAT CGATAGTTAC CGAAACGGGG AGGTGCGCAG ACTGCTGCAG 
GCGCAATTTC CGGAGCTTAA GCTGCCGGAA GAGCGTCATA CCGGGCCGGA GATTGACATC 
CAAACTCTTC AAAGGACTCT ACGGGACGGG GACGAAACAC TGTTTGAGCA GCAGTTCGCT 
GAGTACTTGC AGAATCTCAA AGGCGGAGCG GATAACCAAC TAAATGCCCA CCAGGAGGAA 
TACTTCGGAC TGCTGCAGGA GAGCATCAAG AGGGGCAGGC AGCGAGCCTT CGATGTCATT 
TTGTCCACTG GCATGGATAT CAACTCGAGA CCAGGCAGGG CCAACGAGGC CAATCTCGTA 
GAGACGGCCG TGATATACGG TAACTGGCAG GCGTTGGAGC GACTGCTTAA GGAGCCAAAC 
CTGCGACTTA CTCCAGACTC CAAGCTACTA AATGCAGTAA TCGGCCGTCT GGATGAGCCA 
CCGTATGATG GCTCCAGCCA CCAGCGCTGC TTTGAATTGC TCATTAACAG CGATCGCGTA 
GACATCAACG AAGCTGATTC CGGACGCCTG GTGCCTCTGT TCTTCGCTGT TAAGTACCGC 
AACACGAGTG CGATGCAAAA ACTCCTGAAG AACGGTGCCT ACATTGGTTC TAAGAGCGCA 
TTTGGCACAC TACCCATCAA GGACATGCCA CCCGAGGTTC TCGAAGAGCA CTTCGACTCG 
TGTATCACCA CAAACGGAGA GAGGCCTGGT GACCAGAACT TTGAGATCAT CATCGATTAT 
AAGAACCTAA TGCGCCAGGA GAGAGACTCC GGACTCAACC AGCTGCAAGA CGAAATGGCC 
CCGATCGCAT TCATCGCCGA GTCGAAGGAG ATGCGCCACC TGCTCCAGCA CCCGCTGATC 
TCGAGCTTTC TATTCCTCAA GTGGCACCGA CTTTCCGTGA TATTCTACCT GAACTTCCTG 
ATATACTCGC TTTTTACCGC CTCCATAATT ACCTACACGC TCCTCAAGTT CCACGAAAGC 
GATCAAAGGG CTCTTACTGC ATTTTTCGGA TTGCTTTCCT GGCTGGGAAT CAGCTACCTT 
ATATTACGGG AGTGCATCCA GTGGATAATG TCTCCAGTTC GGTACTTTTG GTCTATAACG 
AATATTATGG AGGTGGCTCT TATTACACTA TCTATCTTTA CCTGCATGGA ATCCAGCTTC 
GACAAGGAGA CGCAGCGCGT CTTAGCCGTA TTTACCATCC TACTCGTCTC CATGGAGTTT 
TGTTTACTAG TGGGCTCCCT GCCAGTGCTC TCAATTTCGA CGCACATGCT GATGCTGCGA 
GAGGTGTCAA ACAGCTTCTT AAAGAGCTTT ACCCTCTACT CGATCTTCGT GCTCACCTTC 
AGCCTGTGTT TCTATATCCT CTTCGGCAAG TCAGTGGAGG AAGACCAGTC TAAAAGCGCT 
ACGCCATGTC CACCTCTGGG GAAGAAGGAG GGGAAGGACG AGGAACAGGG CTTCAACACA 
TTTACCAAGC CTATCGAGGC CGTGATCAAG ACCATTGTGA TGCTGACAGG CGAGTTTGAC 
GCCGGAAGCA TCCAGTTTAC CAGCATCTAC ACCTACCTGA TTTTCCTGCT CTTCGTGATC 
TTTATGACGA TAGTGCTGTT CAACCTTTTG AACGGTCTTG CAGTGAGCGA CACCCAAGTT 
ATTAAGGCTC AGGCGGAACT GAACGGAGCC ATTTGCAGAA CCAACGTCCT TAGTCGGTAC 
GAGCAGGTTC TCACTGGCCA CGGACGCGCT GGGTTTTTGT TGGGCAACCA TCTCTTCCGC 
AGCATCTGCC AACGTTTGAT GAACATCTAC CCGAACTACT TAAGTCTGCG TCAGATTTCC 
GTGCTGCCGA ACGATGGAAA CAAAGTGCTT ATTCCAATGA GCGATCCCTT CGAAATGAGG 
ACCCTTAAGA AGGCTAGCTT TCAGCAATTG CCCCTGAGTG CTGCAGTGCC CCAGAAGAAG 
CTGTTGGATC CACCGCTTAG ACTTCTGCCC TGCTGCTGTT CCCTGCTCAC CGGAAAGTGC 
TCCCAGATGA GCGGCCGGGT GGTCAAACGG GCCCTCGAGG TAATCGATCA GAAGAACGCG 
GCGGAGCAGA GGCGGAAACA GGAACAGATC AACGACAGTC GACTGAAGCT GATCGAGTAC 
AAGCTGGAGC AATTAATACA GCTGGTCCAG GACCGGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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pain cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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