Eps-15 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eps-15
Entrez Gene ID	37961
Full Name	Epidermal growth factor receptor pathway substrate clone 15
Synonyms	CG16932,Dmel\CG16932,EH1,EPS-15,EPS15,Eps15,anon-WO0118547.85,eps-15,eps15,i31
General protein information	Preferred Names Epidermal growth factor receptor pathway substrate clone 15 Names CG16932 gene product from transcript CG16932-RC CG16932-PA CG16932-PB CG16932-PC CG16932-PD Eps-15-PA Eps-15-PB Eps-15-PC Eps-15-PD Eps15 homologue 1 epidermal growth factor receptor pathway substrate clone 15
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 60E1-60E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001259573.1	NP_001246502.1	epidermal growth factor receptor pathway substrate clone 15, isoform D
NM_166687.1	NP_726482.1	epidermal growth factor receptor pathway substrate clone 15, isoform B
NM_138121.2	NP_611965.2	epidermal growth factor receptor pathway substrate clone 15, isoform A
NM_166686.1	NP_726481.1	epidermal growth factor receptor pathway substrate clone 15, isoform C

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	ehs-1	NP_001022499.2
Canis lupus familiaris (dog)	EPS15L1	XP_541965.3
Bos taurus (cattle)	EPS15L1	NP_001095341.1
Danio rerio (zebrafish)	eps15l1a	XP_696575.4
Pan troglodytes (chimpanzee)	EPS15L1	XP_003953451.1
Mus musculus (house mouse)	Eps15l1	NP_031970.2
Gallus gallus (chicken)	EPS15L1	XP_004948994.1
Drosophila melanogaster (fruit fly)	Eps-15	NP_001246502.1
Homo sapiens (human)	EPS15L1	NP_001245303.1
	EPS15L1	XP_001113811.2
Rattus norvegicus (Norway rat)	Eps15l1	NP_001025092.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009189	XP_319960.4
Xenopus tropicalis (tropical clawed frog)	eps15l1	NP_001121513.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Eps-15 controls synaptic vesicle membrane retrieval and synapse development.
Title: Eps15 and Dap160 control synaptic vesicle membrane retrieval and synapse development.

Genetic and biochemical studies of interactions with components of the endocytic machinery suggest that Eps15 has an important role in synaptic vesicle recycling and regulates recruitment of alpha-Adaptin.
Title: Drosophila homologue of Eps15 is essential for synaptic vesicle recycling.

The following Eps-15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eps-15 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13247	NM_001259573.1 Latest version!	Drosophila melanogaster epidermal growth factor receptor pathway substrate clone 15, transcript variant D (Eps-15), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa11232	NM_166686.1 Latest version!	Drosophila melanogaster epidermal growth factor receptor pathway substrate clone 15, transcript variant C (Eps-15), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa11233	NM_166687.1 Latest version!	Drosophila melanogaster epidermal growth factor receptor pathway substrate clone 15, transcript variant B (Eps-15), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa11234	NM_138121.2 Latest version!	Drosophila melanogaster epidermal growth factor receptor pathway substrate clone 15, transcript variant A (Eps-15), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eps-15 ( NM_001259573.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246502.1 Drosophila melanogaster epidermal growth factor receptor pathway substrate clone 15, transcript variant D (Eps-15), mRNA.

Price & Availability↑

CloneID	OFa13247
Clone ID Related Accession (Same CDS sequence)	NM_001259573.1
Accession Version	NM_001259573.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	epidermal growth factor receptor pathway substrate clone 15, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATGTGG ACTTTGCGAG GGTGTGCGGG AAGCACATAG GCGTCTACGA GGCCTACTAC 
AAACTGATTG ACCCCAAAGG CACAGGGGCA ATCGAGGCCA TGACTGCGGC CAAGTTTCTC 
AAGAAGTCGG GCCTTAGCGA CGTTGTGCTA AGCAGGATCT GGGACCTGTC TGATCCGAGC 
GGCAAGGGCT TTCTGGACAA GCCGGGCTTT TTTGTAGCCC TTAAGCTGGT GTCCCTGTCG 
CAGGCGGGAC AGGTGGCCAG CATGGCCAAC ATCTACCTGG ACATTGCCAA CCCGCCCAAG 
TTGGGTGAAC TACCAAAAAC GATGCCGTCG CGTATTCAGA CTGTTCCCGT TGCTAGCGGC 
GGAGTGGCAA ACGGAGACTG GTCCATCGGG GTGATCGATC GGCTCAAGTA CGAGCAGCTC 
TTCGAGTCCC TGCATCCCAG TAATGGAATG CTGCCGGGCA ACAAGGTTAA AGGCGTGCTC 
ATGGACTCCA AGCTGCCCAT GAGCATACTG GGAACTATAT GGGACCTGGC CGACCAGGAC 
AAGGACGGCA ACCTGGATAT GCACGAATTC GTGGTTGCCA TGCACCTGGT CTACCAGACG 
CTGCAGAAGC GCACCATTCC TAGCGTTCTG CCGCCCGAGC TGCGAAAGCC CGGAGGAGCA 
GGTCCGCCAC CGAAGCCAGC CATGCCACCA CCGCCAGCGG GTGCTGCTAT GCCACGTGCT 
CCCAGTGGGG AAGGATTTGG GGACGGCGGA TTTGTAGCAA ATTTTCCCAA GGATATAGCC 
CCGCCGGCGG CTATTCCGCC GCTCCCGGTG GCTGTGCCCC CCATGACGCG CATTCCCCCG 
GTGGGAGCAG TCAGCTCTCA GCCGCTGATT CAGACGGATC CATTGATACC GATTGGGGCT 
CCGGTGATGG CGAATGCCGA CTGGGTTGTC ACCCCAGCGG ACCTAAAGCG GTTCGAGGAG 
ATCTTCCGGC AATCGGATCT GGATAAGGAC GGCCTGGTCT CCGGCCTTGA GGTGAAGGAT 
ATTTTCATCA AGTCGGGTAT TCCGCAGCGA AGCCTAGCAG ACATCTGGGC TCTTTGTGAC 
ACCAATCAGT CCGGTAAGCT GACTGTGGAG CAGTTTGCAC TGGCCATGTG GTTCGTGGAG 
CGAAAGCAGC GCGGTGTGGA CCCGCCACAT GTCCTCAACG CCAATATGGT GCCGCCCTCA 
ATGCGCGCCA CTGTGGCTGG AGTCGATCTG CAGCCGCAGG AGGTCAAGCC CACTTACTCA 
AACCCGGAGC TGGAAATGAT CTCCAAGGAG ATTGAGGAGT TGGCCCGTGA GCGGCGGGTT 
CTGGAGACGG AGATTGCCCA AAAGGAAGCG GATGTGCGCA TCAAAAACGG CGAAGTGCGC 
AGTTTGCAGA GTGAACTGGA TACTCTTACC GCCACACTGA AGCAGCTGGA GAACCAGCGA 
GGAGAGGCCC AGAAGCGACT GGATGACCTG CAGGCTCAAG TGACCAAGAT TCGCGACCAG 
TGCCACATGC AGGAGGTGAC CATCAACGAG CAGGAGGGCG AATTAAACGC AAAACGCTCG 
GAGCTGCAGA AGCTCAAGGA CGAAGAAGCT TCGTTGCAAA AGGAGTACGA CAGCAACAAT 
CGGGAGTTAT CCAAGCTAAC CAACCACCTG CAGGCAACCC AACTGCAAAT CAGCTCGGTC 
CGATCAATGG TCACACAGCT GCTGGAGACG CAGCGACAGA TGACCGACGC TTTGCTCATT 
TGTCGGGCTG CCATGGAGAA CCAAAACGCG GAACTCGTAT CCGAATACCA GTTGAAGATA 
GAGCCGGACT TTGATGAGGC GCGCAAGACC CTGACAAAGG AAGTGCAACT GCCCAAGGAT 
GATCCCTTTG AGGAGAACAA CAGCGGCGCC GCCAATCAAG CTACCAACGG CTTTGGCAGC 
GATCCCTTCT CCGGTCAGCC GGTCAACAAG CCGGCTATTT CTACAGGCTT TGATGACAGC 
TTCAACATGA GCTCAGGCTT CGATAGTGGC TTCGATGCCT TTGGTCAGAG CGGAGCGGGC 
TCAGCCTTTG GTCAGACCCA GCGGGATCCA TTCGGCTCGG ATGCCTTTGC AGCCAACAAG 
AGCAACGCCA TTACTCCTGA GCCCGGCAAG GACGACTTCG GCAGTGATCC GTTTGCTGCC 
TTGCATGCTC CAACCGGCCA GGGTCAGGTG CTCAGTCCCA ACGCCCAGAA GTCCGGACCG 
CCGCCCAGAC CGGAGTCTCC TAGTCCAGCA TTGCCGCCAA AGAAGTCCAA GGTGCCGCCC 
CCACGACCAG CGCCACCACG AGCAGCCCAG CCCACGGGTG GTTTCGGAAG CGGCGGCGGA 
GGAGGATTTG CCGACTTTGA CGACTTCGAC AATAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246502.1
CDS	293..2671
Translation	MNVDFARVCGKHIGVYEAYYKLIDPKGTGAIEAMTAAKFLKKSGLSDVVLSRIWDLSDPSGKGFLDKPGFFVALKLVSLSQAGQVASMANIYLDIANPPKLGELPKTMPSRIQTVPVASGGVANGDWSIGVIDRLKYEQLFESLHPSNGMLPGNKVKGVLMDSKLPMSILGTIWDLADQDKDGNLDMHEFVVAMHLVYQTLQKRTIPSVLPPELRKPGGAGPPPKPAMPPPPAGAAMPRAPSGEGFGDGGFVANFPKDIAPPAAIPPLPVAVPPMTRIPPVGAVSSQPLIQTDPLIPIGAPVMANADWVVTPADLKRFEEIFRQSDLDKDGLVSGLEVKDIFIKSGIPQRSLADIWALCDTNQSGKLTVEQFALAMWFVERKQRGVDPPHVLNANMVPPSMRATVAGVDLQPQEVKPTYSNPELEMISKEIEELARERRVLETEIAQKEADVRIKNGEVRSLQSELDTLTATLKQLENQRGEAQKRLDDLQAQVTKIRDQCHMQEVTINEQEGELNAKRSELQKLKDEEASLQKEYDSNNRELSKLTNHLQATQLQISSVRSMVTQLLETQRQMTDALLICRAAMENQNAELVSEYQLKIEPDFDEARKTLTKEVQLPKDDPFEENNSGAANQATNGFGSDPFSGQPVNKPAISTGFDDSFNMSSGFDSGFDAFGQSGAGSAFGQTQRDPFGSDAFAANKSNAITPEPGKDDFGSDPFAALHAPTGQGQVLSPNAQKSGPPPRPESPSPALPPKKSKVPPPRPAPPRAAQPTGGFGSGGGGGFADFDDFDNK

Target ORF information:

RefSeq Version	NM_001259573.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster epidermal growth factor receptor pathway substrate clone 15, transcript variant D (Eps-15), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259573.1

ORF Insert Sequence:

ATGAATGTGG ACTTTGCGAG GGTGTGCGGG AAGCACATAG GCGTCTACGA GGCCTACTAC 
AAACTGATTG ACCCCAAAGG CACAGGGGCA ATCGAGGCCA TGACTGCGGC CAAGTTTCTC 
AAGAAGTCGG GCCTTAGCGA CGTTGTGCTA AGCAGGATCT GGGACCTGTC TGATCCGAGC 
GGCAAGGGCT TTCTGGACAA GCCGGGCTTT TTTGTAGCCC TTAAGCTGGT GTCCCTGTCG 
CAGGCGGGAC AGGTGGCCAG CATGGCCAAC ATCTACCTGG ACATTGCCAA CCCGCCCAAG 
TTGGGTGAAC TACCAAAAAC GATGCCGTCG CGTATTCAGA CTGTTCCCGT TGCTAGCGGC 
GGAGTGGCAA ACGGAGACTG GTCCATCGGG GTGATCGATC GGCTCAAGTA CGAGCAGCTC 
TTCGAGTCCC TGCATCCCAG TAATGGAATG CTGCCGGGCA ACAAGGTTAA AGGCGTGCTC 
ATGGACTCCA AGCTGCCCAT GAGCATACTG GGAACTATAT GGGACCTGGC CGACCAGGAC 
AAGGACGGCA ACCTGGATAT GCACGAATTC GTGGTTGCCA TGCACCTGGT CTACCAGACG 
CTGCAGAAGC GCACCATTCC TAGCGTTCTG CCGCCCGAGC TGCGAAAGCC CGGAGGAGCA 
GGTCCGCCAC CGAAGCCAGC CATGCCACCA CCGCCAGCGG GTGCTGCTAT GCCACGTGCT 
CCCAGTGGGG AAGGATTTGG GGACGGCGGA TTTGTAGCAA ATTTTCCCAA GGATATAGCC 
CCGCCGGCGG CTATTCCGCC GCTCCCGGTG GCTGTGCCCC CCATGACGCG CATTCCCCCG 
GTGGGAGCAG TCAGCTCTCA GCCGCTGATT CAGACGGATC CATTGATACC GATTGGGGCT 
CCGGTGATGG CGAATGCCGA CTGGGTTGTC ACCCCAGCGG ACCTAAAGCG GTTCGAGGAG 
ATCTTCCGGC AATCGGATCT GGATAAGGAC GGCCTGGTCT CCGGCCTTGA GGTGAAGGAT 
ATTTTCATCA AGTCGGGTAT TCCGCAGCGA AGCCTAGCAG ACATCTGGGC TCTTTGTGAC 
ACCAATCAGT CCGGTAAGCT GACTGTGGAG CAGTTTGCAC TGGCCATGTG GTTCGTGGAG 
CGAAAGCAGC GCGGTGTGGA CCCGCCACAT GTCCTCAACG CCAATATGGT GCCGCCCTCA 
ATGCGCGCCA CTGTGGCTGG AGTCGATCTG CAGCCGCAGG AGGTCAAGCC CACTTACTCA 
AACCCGGAGC TGGAAATGAT CTCCAAGGAG ATTGAGGAGT TGGCCCGTGA GCGGCGGGTT 
CTGGAGACGG AGATTGCCCA AAAGGAAGCG GATGTGCGCA TCAAAAACGG CGAAGTGCGC 
AGTTTGCAGA GTGAACTGGA TACTCTTACC GCCACACTGA AGCAGCTGGA GAACCAGCGA 
GGAGAGGCCC AGAAGCGACT GGATGACCTG CAGGCTCAAG TGACCAAGAT TCGCGACCAG 
TGCCACATGC AGGAGGTGAC CATCAACGAG CAGGAGGGCG AATTAAACGC AAAACGCTCG 
GAGCTGCAGA AGCTCAAGGA CGAAGAAGCT TCGTTGCAAA AGGAGTACGA CAGCAACAAT 
CGGGAGTTAT CCAAGCTAAC CAACCACCTG CAGGCAACCC AACTGCAAAT CAGCTCGGTC 
CGATCAATGG TCACACAGCT GCTGGAGACG CAGCGACAGA TGACCGACGC TTTGCTCATT 
TGTCGGGCTG CCATGGAGAA CCAAAACGCG GAACTCGTAT CCGAATACCA GTTGAAGATA 
GAGCCGGACT TTGATGAGGC GCGCAAGACC CTGACAAAGG AAGTGCAACT GCCCAAGGAT 
GATCCCTTTG AGGAGAACAA CAGCGGCGCC GCCAATCAAG CTACCAACGG CTTTGGCAGC 
GATCCCTTCT CCGGTCAGCC GGTCAACAAG CCGGCTATTT CTACAGGCTT TGATGACAGC 
TTCAACATGA GCTCAGGCTT CGATAGTGGC TTCGATGCCT TTGGTCAGAG CGGAGCGGGC 
TCAGCCTTTG GTCAGACCCA GCGGGATCCA TTCGGCTCGG ATGCCTTTGC AGCCAACAAG 
AGCAACGCCA TTACTCCTGA GCCCGGCAAG GACGACTTCG GCAGTGATCC GTTTGCTGCC 
TTGCATGCTC CAACCGGCCA GGGTCAGGTG CTCAGTCCCA ACGCCCAGAA GTCCGGACCG 
CCGCCCAGAC CGGAGTCTCC TAGTCCAGCA TTGCCGCCAA AGAAGTCCAA GGTGCCGCCC 
CCACGACCAG CGCCACCACG AGCAGCCCAG CCCACGGGTG GTTTCGGAAG CGGCGGCGGA 
GGAGGATTTG CCGACTTTGA CGACTTCGAC AATAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eps-15 ( NM_166686.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726481.1 Drosophila melanogaster epidermal growth factor receptor pathway substrate clone 15, transcript variant C (Eps-15), mRNA.

Price & Availability↑

CloneID	OFa11232
Clone ID Related Accession (Same CDS sequence)	NM_166686.1
Accession Version	NM_166686.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	epidermal growth factor receptor pathway substrate clone 15, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATGTGG ACTTTGCGAG GGTGTGCGGG AAGCACATAG GCGTCTACGA GGCCTACTAC 
AAACTGATTG ACCCCAAAGG CACAGGGGCA ATCGAGGCCA TGACTGCGGC CAAGTTTCTC 
AAGAAGTCGG GCCTTAGCGA CGTTGTGCTA AGCAGGATCT GGGACCTGTC TGATCCGAGC 
GGCAAGGGCT TTCTGGACAA GCCGGGCTTT TTTGTAGCCC TTAAGCTGGT GTCCCTGTCG 
CAGGCGGGAC AGGTGGCCAG CATGGCCAAC ATCTACCTGG ACATTGCCAA CCCGCCCAAG 
TTGGGTGAAC TACCAAAAAC GATGCCGTCG CGTATTCAGA CTGTTCCCGT TGCTAGCGGC 
GGAGTGGCAA ACGGAGACTG GTCCATCGGG GTGATCGATC GGCTCAAGTA CGAGCAGCTC 
TTCGAGTCCC TGCATCCCAG TAATGGAATG CTGCCGGGCA ACAAGGTTAA AGGCGTGCTC 
ATGGACTCCA AGCTGCCCAT GAGCATACTG GGAACTATAT GGGACCTGGC CGACCAGGAC 
AAGGACGGCA ACCTGGATAT GCACGAATTC GTGGTTGCCA TGCACCTGGT CTACCAGACG 
CTGCAGAAGC GCACCATTCC TAGCGTTCTG CCGCCCGAGC TGCGAAAGCC CGGAGGAGCA 
GGTCCGCCAC CGAAGCCAGC CATGCCACCA CCGCCAGCGG GTGCTGCTAT GCCACGTGCT 
CCCAGTGGGG AAGGATTTGG GGACGGCGGA TTTGTAGCAA ATTTTCCCAA GGATATAGCC 
CCGCCGGCGG CTATTCCGCC GCTCCCGGTG GCTGTGCCCC CCATGACGCG CATTCCCCCG 
GTGGGAGCAG TCAGCTCTCA GCCGCTGATT CAGACGGATC CATTGATACC GATTGGGGCT 
CCGGTGATGG CGAATGCCGA CTGGGTTGTC ACCCCAGCGG ACCTAAAGCG GTTCGAGGAG 
ATCTTCCGGC AATCGGATCT GGATAAGGAC GGCCTGGTCT CCGGCCTTGA GGTGAAGGAT 
ATTTTCATCA AGTCGGGTAT TCCGCAGCGA AGCCTAGCAG ACATCTGGGC TCTTTGTGAC 
ACCAATCAGT CCGGTAAGCT GACTGTGGAG CAGTTTGCAC TGGCCATGTG GTTCGTGGAG 
CGAAAGCAGC GCGGTGTGGA CCCGCCACAT GTCCTCAACG CCAATATGGT GCCGCCCTCA 
ATGCGCGCCA CTGTGGCTGG AGTCGATCTG CAGCCGCAGG AGGTCAAGCC CACTTACTCA 
AACCCGGAGC TGGAAATGAT CTCCAAGGAG ATTGAGGAGT TGGCCCGTGA GCGGCGGGTT 
CTGGAGACGG AGATTGCCCA AAAGGAAGCG GATGTGCGCA TCAAAAACGG CGAAGTGCGC 
AGTTTGCAGA GTGAACTGGA TACTCTTACC GCCACACTGA AGCAGCTGGA GAACCAGCGA 
GGAGAGGCCC AGAAGCGACT GGATGACCTG CAGGCTCAAG TGACCAAGAT TCGCGACCAG 
TGCCACATGC AGGAGGTGAC CATCAACGAG CAGGAGGGCG AATTAAACGC AAAACGCTCG 
GAGCTGCAGA AGCTCAAGGA CGAAGAAGCT TCGTTGCAAA AGGAGTACGA CAGCAACAAT 
CGGGAGTTAT CCAAGCTAAC CAACCACCTG CAGGCAACCC AACTGCAAAT CAGCTCGGTC 
CGATCAATGG TCACACAGCT GCTGGAGACG CAGCGACAGA TGACCGACGC TTTGCTCATT 
TGTCGGGCTG CCATGGAGAA CCAAAACGCG GAACTCGTAT CCGAATACCA GTTGAAGATA 
GAGCCGGACT TTGATGAGGC GCGCAAGACC CTGACAAAGG AAGTGCAACT GCCCAAGGAT 
GATCCCTTTG AGGAGAACAA CAGCGGCGCC GCCAATCAAG CTACCAACGG CTTTGGCAGC 
GATCCCTTCT CCGGTCAGCC GGTCAACAAG CCGGCTATTT CTACAGGCTT TGATGACAGC 
TTCAACATGA GCTCAGGCTT CGATAGTGGC TTCGATGCCT TTGGTCAGAG CGGAGCGGGC 
TCAGCCTTTG GTCAGACCCA GCGGGATCCA TTCGGCTCGG ATGCCTTTGC AGCCAACAAG 
AGCAACGCCA TTACTCCTGA GCCCGGCAAG GACGACTTCG GCAGTGATCC GTTTGCTGCC 
TTGCATGCTC CAACCGGCCA GGGTCAGGTG CTCAGTCCCA ACGCCCAGAA GTCCGGACCG 
CCGCCCAGAC CGGAGTCTCC TAGTCCAGCA TTGCCGCCAA AGAAGTCCAA GGTGCCGCCC 
CCACGACCAG CGCCACCACG AGCAGCCCAG CCCACGGGTG GTTTCGGAAG CGGCGGCGGA 
GGAGGATTTG CCGACTTTGA CGACTTCGAC AATAAGAACC AGAGGACGGC GGATGTGAAA 
TTGGAGACCA AGGCGGTAGT CAGTGTGTTT GATGCATTTG GAGAGATTGG AACTCGGAAG 
GCCCCGACTC CCAGTCTCAT TACAGGGCCA ACCGACTTCA AGGATGACCC CTTCAAGGAT 
TACCGTTACG AAGATCCATT TAGCATCAAG GATCCTTTTG CCGAAGAGGG CGAGGAGGAG 
TTGTCAGAAG GCAAGGGTGC TGACCACAAG AGGAACTTTG CGGAGGACTT TAGCTCGGGT 
GATGAGATTG GATCAGCCGC CAAAACTCTG AGTAACATCG TGAACAATAA TAGCATTAAG 
CCCAAGGCGG CCACGCCGCT AAACAACGAC CTGCTGCAGC AGTTCGACGC CTTCAACTTG 
AACTCGAGTC CGGCGCCGTC GCACCACTCA GGCACTTCGT ACCACTCCAA CTCCAAGCCG 
AGCCACGACT CGCAGACGGC GCTGGACGCA TTCGCTGACT TCGACGACTT CGCAGCTACG 
CTGGCAACCA CTGGTGGATC TGGGGCTGCA ACCACGGCCA CAACCACTGG CAATACGAAA 
CTCAACAATT CCTTTTTTGA TGCATTTAAC GACAACTTCA GTGTGATCCA GAGCTCATCA 
GTACCCACTG GCGTGGTCAG GCCCAACGAT CAAAGGGCAA AGGCATTTGA CGCCTTCAAC 
GACAATTTCG AGGACCAATT CAAGACAAGC CCCAACGCCA ACTTCGCCAA GTTCGATGGC 
TTCGAGGCAC ACAACTTCTC GAGTGACTTC GGAAACGCCT CCTTCGGAAC GACGGGCAAG 
GAGAAGCAGA ACGGGCAGGT GGACAAGAAG TTTCAGAGCA AGGAGCCGGT CAAGGACAAG 
TTTGCTGCAG ACTATTCCAA GCCGGAGAGC TTCGACGCGG ACCTGGAGGA GGCGCTGAAG 
CGCAGCGTAC TGGACAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726481.1
CDS	293..3613
Translation	MNVDFARVCGKHIGVYEAYYKLIDPKGTGAIEAMTAAKFLKKSGLSDVVLSRIWDLSDPSGKGFLDKPGFFVALKLVSLSQAGQVASMANIYLDIANPPKLGELPKTMPSRIQTVPVASGGVANGDWSIGVIDRLKYEQLFESLHPSNGMLPGNKVKGVLMDSKLPMSILGTIWDLADQDKDGNLDMHEFVVAMHLVYQTLQKRTIPSVLPPELRKPGGAGPPPKPAMPPPPAGAAMPRAPSGEGFGDGGFVANFPKDIAPPAAIPPLPVAVPPMTRIPPVGAVSSQPLIQTDPLIPIGAPVMANADWVVTPADLKRFEEIFRQSDLDKDGLVSGLEVKDIFIKSGIPQRSLADIWALCDTNQSGKLTVEQFALAMWFVERKQRGVDPPHVLNANMVPPSMRATVAGVDLQPQEVKPTYSNPELEMISKEIEELARERRVLETEIAQKEADVRIKNGEVRSLQSELDTLTATLKQLENQRGEAQKRLDDLQAQVTKIRDQCHMQEVTINEQEGELNAKRSELQKLKDEEASLQKEYDSNNRELSKLTNHLQATQLQISSVRSMVTQLLETQRQMTDALLICRAAMENQNAELVSEYQLKIEPDFDEARKTLTKEVQLPKDDPFEENNSGAANQATNGFGSDPFSGQPVNKPAISTGFDDSFNMSSGFDSGFDAFGQSGAGSAFGQTQRDPFGSDAFAANKSNAITPEPGKDDFGSDPFAALHAPTGQGQVLSPNAQKSGPPPRPESPSPALPPKKSKVPPPRPAPPRAAQPTGGFGSGGGGGFADFDDFDNKNQRTADVKLETKAVVSVFDAFGEIGTRKAPTPSLITGPTDFKDDPFKDYRYEDPFSIKDPFAEEGEEELSEGKGADHKRNFAEDFSSGDEIGSAAKTLSNIVNNNSIKPKAATPLNNDLLQQFDAFNLNSSPAPSHHSGTSYHSNSKPSHDSQTALDAFADFDDFAATLATTGGSGAATTATTTGNTKLNNSFFDAFNDNFSVIQSSSVPTGVVRPNDQRAKAFDAFNDNFEDQFKTSPNANFAKFDGFEAHNFSSDFGNASFGTTGKEKQNGQVDKKFQSKEPVKDKFAADYSKPESFDADLEEALKRSVLDN

Target ORF information:

RefSeq Version	NM_166686.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster epidermal growth factor receptor pathway substrate clone 15, transcript variant C (Eps-15), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166686.1

ORF Insert Sequence:

ATGAATGTGG ACTTTGCGAG GGTGTGCGGG AAGCACATAG GCGTCTACGA GGCCTACTAC 
AAACTGATTG ACCCCAAAGG CACAGGGGCA ATCGAGGCCA TGACTGCGGC CAAGTTTCTC 
AAGAAGTCGG GCCTTAGCGA CGTTGTGCTA AGCAGGATCT GGGACCTGTC TGATCCGAGC 
GGCAAGGGCT TTCTGGACAA GCCGGGCTTT TTTGTAGCCC TTAAGCTGGT GTCCCTGTCG 
CAGGCGGGAC AGGTGGCCAG CATGGCCAAC ATCTACCTGG ACATTGCCAA CCCGCCCAAG 
TTGGGTGAAC TACCAAAAAC GATGCCGTCG CGTATTCAGA CTGTTCCCGT TGCTAGCGGC 
GGAGTGGCAA ACGGAGACTG GTCCATCGGG GTGATCGATC GGCTCAAGTA CGAGCAGCTC 
TTCGAGTCCC TGCATCCCAG TAATGGAATG CTGCCGGGCA ACAAGGTTAA AGGCGTGCTC 
ATGGACTCCA AGCTGCCCAT GAGCATACTG GGAACTATAT GGGACCTGGC CGACCAGGAC 
AAGGACGGCA ACCTGGATAT GCACGAATTC GTGGTTGCCA TGCACCTGGT CTACCAGACG 
CTGCAGAAGC GCACCATTCC TAGCGTTCTG CCGCCCGAGC TGCGAAAGCC CGGAGGAGCA 
GGTCCGCCAC CGAAGCCAGC CATGCCACCA CCGCCAGCGG GTGCTGCTAT GCCACGTGCT 
CCCAGTGGGG AAGGATTTGG GGACGGCGGA TTTGTAGCAA ATTTTCCCAA GGATATAGCC 
CCGCCGGCGG CTATTCCGCC GCTCCCGGTG GCTGTGCCCC CCATGACGCG CATTCCCCCG 
GTGGGAGCAG TCAGCTCTCA GCCGCTGATT CAGACGGATC CATTGATACC GATTGGGGCT 
CCGGTGATGG CGAATGCCGA CTGGGTTGTC ACCCCAGCGG ACCTAAAGCG GTTCGAGGAG 
ATCTTCCGGC AATCGGATCT GGATAAGGAC GGCCTGGTCT CCGGCCTTGA GGTGAAGGAT 
ATTTTCATCA AGTCGGGTAT TCCGCAGCGA AGCCTAGCAG ACATCTGGGC TCTTTGTGAC 
ACCAATCAGT CCGGTAAGCT GACTGTGGAG CAGTTTGCAC TGGCCATGTG GTTCGTGGAG 
CGAAAGCAGC GCGGTGTGGA CCCGCCACAT GTCCTCAACG CCAATATGGT GCCGCCCTCA 
ATGCGCGCCA CTGTGGCTGG AGTCGATCTG CAGCCGCAGG AGGTCAAGCC CACTTACTCA 
AACCCGGAGC TGGAAATGAT CTCCAAGGAG ATTGAGGAGT TGGCCCGTGA GCGGCGGGTT 
CTGGAGACGG AGATTGCCCA AAAGGAAGCG GATGTGCGCA TCAAAAACGG CGAAGTGCGC 
AGTTTGCAGA GTGAACTGGA TACTCTTACC GCCACACTGA AGCAGCTGGA GAACCAGCGA 
GGAGAGGCCC AGAAGCGACT GGATGACCTG CAGGCTCAAG TGACCAAGAT TCGCGACCAG 
TGCCACATGC AGGAGGTGAC CATCAACGAG CAGGAGGGCG AATTAAACGC AAAACGCTCG 
GAGCTGCAGA AGCTCAAGGA CGAAGAAGCT TCGTTGCAAA AGGAGTACGA CAGCAACAAT 
CGGGAGTTAT CCAAGCTAAC CAACCACCTG CAGGCAACCC AACTGCAAAT CAGCTCGGTC 
CGATCAATGG TCACACAGCT GCTGGAGACG CAGCGACAGA TGACCGACGC TTTGCTCATT 
TGTCGGGCTG CCATGGAGAA CCAAAACGCG GAACTCGTAT CCGAATACCA GTTGAAGATA 
GAGCCGGACT TTGATGAGGC GCGCAAGACC CTGACAAAGG AAGTGCAACT GCCCAAGGAT 
GATCCCTTTG AGGAGAACAA CAGCGGCGCC GCCAATCAAG CTACCAACGG CTTTGGCAGC 
GATCCCTTCT CCGGTCAGCC GGTCAACAAG CCGGCTATTT CTACAGGCTT TGATGACAGC 
TTCAACATGA GCTCAGGCTT CGATAGTGGC TTCGATGCCT TTGGTCAGAG CGGAGCGGGC 
TCAGCCTTTG GTCAGACCCA GCGGGATCCA TTCGGCTCGG ATGCCTTTGC AGCCAACAAG 
AGCAACGCCA TTACTCCTGA GCCCGGCAAG GACGACTTCG GCAGTGATCC GTTTGCTGCC 
TTGCATGCTC CAACCGGCCA GGGTCAGGTG CTCAGTCCCA ACGCCCAGAA GTCCGGACCG 
CCGCCCAGAC CGGAGTCTCC TAGTCCAGCA TTGCCGCCAA AGAAGTCCAA GGTGCCGCCC 
CCACGACCAG CGCCACCACG AGCAGCCCAG CCCACGGGTG GTTTCGGAAG CGGCGGCGGA 
GGAGGATTTG CCGACTTTGA CGACTTCGAC AATAAGAACC AGAGGACGGC GGATGTGAAA 
TTGGAGACCA AGGCGGTAGT CAGTGTGTTT GATGCATTTG GAGAGATTGG AACTCGGAAG 
GCCCCGACTC CCAGTCTCAT TACAGGGCCA ACCGACTTCA AGGATGACCC CTTCAAGGAT 
TACCGTTACG AAGATCCATT TAGCATCAAG GATCCTTTTG CCGAAGAGGG CGAGGAGGAG 
TTGTCAGAAG GCAAGGGTGC TGACCACAAG AGGAACTTTG CGGAGGACTT TAGCTCGGGT 
GATGAGATTG GATCAGCCGC CAAAACTCTG AGTAACATCG TGAACAATAA TAGCATTAAG 
CCCAAGGCGG CCACGCCGCT AAACAACGAC CTGCTGCAGC AGTTCGACGC CTTCAACTTG 
AACTCGAGTC CGGCGCCGTC GCACCACTCA GGCACTTCGT ACCACTCCAA CTCCAAGCCG 
AGCCACGACT CGCAGACGGC GCTGGACGCA TTCGCTGACT TCGACGACTT CGCAGCTACG 
CTGGCAACCA CTGGTGGATC TGGGGCTGCA ACCACGGCCA CAACCACTGG CAATACGAAA 
CTCAACAATT CCTTTTTTGA TGCATTTAAC GACAACTTCA GTGTGATCCA GAGCTCATCA 
GTACCCACTG GCGTGGTCAG GCCCAACGAT CAAAGGGCAA AGGCATTTGA CGCCTTCAAC 
GACAATTTCG AGGACCAATT CAAGACAAGC CCCAACGCCA ACTTCGCCAA GTTCGATGGC 
TTCGAGGCAC ACAACTTCTC GAGTGACTTC GGAAACGCCT CCTTCGGAAC GACGGGCAAG 
GAGAAGCAGA ACGGGCAGGT GGACAAGAAG TTTCAGAGCA AGGAGCCGGT CAAGGACAAG 
TTTGCTGCAG ACTATTCCAA GCCGGAGAGC TTCGACGCGG ACCTGGAGGA GGCGCTGAAG 
CGCAGCGTAC TGGACAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eps-15 ( NM_166687.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726482.1 Drosophila melanogaster epidermal growth factor receptor pathway substrate clone 15, transcript variant B (Eps-15), mRNA.

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CloneID	OFa11233
Clone ID Related Accession (Same CDS sequence)	NM_166687.1
Accession Version	NM_166687.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	epidermal growth factor receptor pathway substrate clone 15, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATGTGG ACTTTGCGAG GGTGTGCGGG AAGCACATAG GCGTCTACGA GGCCTACTAC 
AAACTGATTG ACCCCAAAGG CACAGGGGCA ATCGAGGCCA TGACTGCGGC CAAGTTTCTC 
AAGAAGTCGG GCCTTAGCGA CGTTGTGCTA AGCAGGATCT GGGACCTGTC TGATCCGAGC 
GGCAAGGGCT TTCTGGACAA GCCGGGCTTT TTTGTAGCCC TTAAGCTGGT GTCCCTGTCG 
CAGGCGGGAC AGGTGGCCAG CATGGCCAAC ATCTACCTGG ACATTGCCAA CCCGCCCAAG 
TTGGGTGAAC TACCAAAAAC GATGCCGTCG CGTATTCAGA CTGTTCCCGT TGCTAGCGGC 
GGAGTGGCAA ACGGAGACTG GTCCATCGGG GTGATCGATC GGCTCAAGTA CGAGCAGCTC 
TTCGAGTCCC TGCATCCCAG TAATGGAATG CTGCCGGGCA ACAAGGTTAA AGGCGTGCTC 
ATGGACTCCA AGCTGCCCAT GAGCATACTG GGAACTATAT GGGACCTGGC CGACCAGGAC 
AAGGACGGCA ACCTGGATAT GCACGAATTC GTGGTTGCCA TGCACCTGGT CTACCAGACG 
CTGCAGAAGC GCACCATTCC TAGCGTTCTG CCGCCCGAGC TGCGAAAGCC CGGAGGAGCA 
GGTCCGCCAC CGAAGCCAGC CATGCCACCA CCGCCAGCGG GTGCTGCTAT GCCACGTGCT 
CCCAGTGGGG AAGGATTTGG GGACGGCGGA TTTGTAGCAA ATTTTCCCAA GGATATAGCC 
CCGCCGGCGG CTATTCCGCC GCTCCCGGTG GCTGTGCCCC CCATGACGCG CATTCCCCCG 
GTGGGAGCAG TCAGCTCTCA GCCGCTGATT CAGACGGATC CATTGATACC GATTGGGGCT 
CCGGTGATGG CGAATGCCGA CTGGGTTGTC ACCCCAGCGG ACCTAAAGCG GTTCGAGGAG 
ATCTTCCGGC AATCGGATCT GGATAAGGAC GGCCTGGTCT CCGGCCTTGA GGTGAAGGAT 
ATTTTCATCA AGTCGGGTAT TCCGCAGCGA AGCCTAGCAG ACATCTGGGC TCTTTGTGAC 
ACCAATCAGT CCGGTAAGCT GACTGTGGAG CAGTTTGCAC TGGCCATGTG GTTCGTGGAG 
CGAAAGCAGC GCGGTGTGGA CCCGCCACAT GTCCTCAACG CCAATATGGT GCCGCCCTCA 
ATGCGCGCCA CTGTGGCTGG AGTCGATCTG CAGCCGCAGG AGGTCAAGCC CACTTACTCA 
AACCCGGAGC TGGAAATGAT CTCCAAGGAG ATTGAGGAGT TGGCCCGTGA GCGGCGGGTT 
CTGGAGACGG AGATTGCCCA AAAGGAAGCG GATGTGCGCA TCAAAAACGG CGAAGTGCGC 
AGTTTGCAGA GTGAACTGGA TACTCTTACC GCCACACTGA AGCAGCTGGA GAACCAGCGA 
GGAGAGGCCC AGAAGCGACT GGATGACCTG CAGGCTCAAG TGACCAAGAT TCGCGACCAG 
TGCCACATGC AGGAGGTGAC CATCAACGAG CAGGAGGGCG AATTAAACGC AAAACGCTCG 
GAGCTGCAGA AGCTCAAGGA CGAAGAAGCT TCGTTGCAAA AGGAGTACGA CAGCAACAAT 
CGGGAGTTAT CCAAGCTAAC CAACCACCTG CAGGCAACCC AACTGCAAAT CAGCTCGGTC 
CGATCAATGG TCACACAGCT GCTGGAGACG CAGCGACAGA TGACCGACGC TTTGCTCATT 
TGTCGGGCTG CCATGGAGAA CCAAAACGCG GAACTCGTAT CCGAATACCA GTTGAAGATA 
GAGCCGGACT TTGATGAGGC GCGCAAGACC CTGACAAAGG AAGTGCAACT GCCCAAGGAT 
GATCCCTTTG AGGAGAACAA CAGCGGCGCC GCCAATCAAG CTACCAACGG CTTTGGCAGC 
GATCCCTTCT CCGGTCAGCC GGTCAACAAG CCGGCTATTT CTACAGGCTT TGATGACAGC 
TTCAACATGA GCTCAGGCTT CGATAGTGGC TTCGATGCCT TTGGTCAGAG CGGAGCGGGC 
TCAGCCTTTG GTCAGACCCA GCGGGATCCA TTCGGCTCGG ATGCCTTTGC AGCCAACAAG 
AGCAACGCCA TTACTCCTGA GCCCGGCAAG GACGACTTCG GCAGTGATCC GTTTGCTGCC 
TTGCATGCTC CAACCGGCCA GGGTCAGGTG CTCAGTCCCA ACGCCCAGAA GTCCGGACCG 
CCGCCCAGAC CGGAGTCTCC TAGTCCAGCA TTGCCGCCAA AGAAGTCCAA GGTGCCGCCC 
CCACGACCAG CGCCACCACG AGCAGCCCAG CCCACGGGTG GTTTCGGAAG CGGCGGCGGA 
GGAGGATTTG CCGACTTTGA CGACTTCGAC AATAAGCTCC ACCACATTCC AAGCGCACCC 
TCGCCCTCGG CCACTGCACT GTCCCCGCTC CCACCCACGC TCCCAGCCCC CATTCCGGTC 
GTCAGTGGAT CGTCATTGCT AGACAGCTTC ACGCTATTCG ACGATCCCGG CCAAATCCAC 
AAGCAGGCGG CAAGCACGCC CAATCCCACG CCCATCACTG TCCCCACCGT ACACACCCTG 
CTCCAGACAT CTTTATCCAC GCCAGCAGCC CCATCCCCGG CATTGGCTTC TCTATCAACA 
TCAGGATCGG GATCGGTAGC GGGAGCGGGG GCAGACCTCC CCAGTAGCGT CACCATTACC 
ACCGCCCCCA GCTTGAATAA CCAGCATCTG TCGCGTTCCA ATACACCGCT GCAGAACCAG 
AGGACGGCGG ATGTGAAATT GGAGACCAAG GCGGTAGTCA GTGTGTTTGA TGCATTTGGA 
GAGATTGGAA CTCGGAAGGC CCCGACTCCC AGTCTCATTA CAGGGCCAAC CGACTTCAAG 
GATGACCCCT TCAAGGATTA CCGTTACGAA GATCCATTTA GCATCAAGGA TCCTTTTGCC 
GAAGAGGGCG AGGAGGAGTT GTCAGAAGGC AAGGGTGCTG ACCACAAGAG GAACTTTGCG 
GAGGACTTTA GCTCGGGTGA TGAGATTGGA TCAGCCGCCA AAACTCTGAG TAACATCGTG 
AACAATAATA GCATTAAGCC CAAGGCGGCC ACGCCGCTAA ACAACGACCT GCTGCAGCAG 
TTCGACGCCT TCAACTTGAA CTCGAGTCCG GCGCCGTCGC ACCACTCAGG CACTTCGTAC 
CACTCCAACT CCAAGCCGAG CCACGACTCG CAGACGGCGC TGGACGCATT CGCTGACTTC 
GACGACTTCG CAGCTACGCT GGCAACCACT GGTGGATCTG GGGCTGCAAC CACGGCCACA 
ACCACTGGCA ATACGAAACT CAACAATTCC TTTTTTGATG CATTTAACGA CAACTTCAGT 
GTGATCCAGA GCTCATCAGT ACCCACTGGC GTGGTCAGGC CCAACGATCA AAGGGCAAAG 
GCATTTGACG CCTTCAACGA CAATTTCGAG GACCAATTCA AGACAAGCCC CAACGCCAAC 
TTCGCCAAGT TCGATGGCTT CGAGGCACAC AACTTCTCGA GTGACTTCGG AAACGCCTCC 
TTCGGAACGA CGGGCAAGGA GAAGCAGAAC GGGCAGGTGG ACAAGAAGTT TCAGAGCAAG 
GAGCCGGTCA AGGACAAGTT TGCTGCAGAC TATTCCAAGC CGGAGAGCTT CGACGCGGAC 
CTGGAGGAGG CGCTGAAGCG CAGCGTACTG GACAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726482.1
CDS	293..3991
Translation	MNVDFARVCGKHIGVYEAYYKLIDPKGTGAIEAMTAAKFLKKSGLSDVVLSRIWDLSDPSGKGFLDKPGFFVALKLVSLSQAGQVASMANIYLDIANPPKLGELPKTMPSRIQTVPVASGGVANGDWSIGVIDRLKYEQLFESLHPSNGMLPGNKVKGVLMDSKLPMSILGTIWDLADQDKDGNLDMHEFVVAMHLVYQTLQKRTIPSVLPPELRKPGGAGPPPKPAMPPPPAGAAMPRAPSGEGFGDGGFVANFPKDIAPPAAIPPLPVAVPPMTRIPPVGAVSSQPLIQTDPLIPIGAPVMANADWVVTPADLKRFEEIFRQSDLDKDGLVSGLEVKDIFIKSGIPQRSLADIWALCDTNQSGKLTVEQFALAMWFVERKQRGVDPPHVLNANMVPPSMRATVAGVDLQPQEVKPTYSNPELEMISKEIEELARERRVLETEIAQKEADVRIKNGEVRSLQSELDTLTATLKQLENQRGEAQKRLDDLQAQVTKIRDQCHMQEVTINEQEGELNAKRSELQKLKDEEASLQKEYDSNNRELSKLTNHLQATQLQISSVRSMVTQLLETQRQMTDALLICRAAMENQNAELVSEYQLKIEPDFDEARKTLTKEVQLPKDDPFEENNSGAANQATNGFGSDPFSGQPVNKPAISTGFDDSFNMSSGFDSGFDAFGQSGAGSAFGQTQRDPFGSDAFAANKSNAITPEPGKDDFGSDPFAALHAPTGQGQVLSPNAQKSGPPPRPESPSPALPPKKSKVPPPRPAPPRAAQPTGGFGSGGGGGFADFDDFDNKLHHIPSAPSPSATALSPLPPTLPAPIPVVSGSSLLDSFTLFDDPGQIHKQAASTPNPTPITVPTVHTLLQTSLSTPAAPSPALASLSTSGSGSVAGAGADLPSSVTITTAPSLNNQHLSRSNTPLQNQRTADVKLETKAVVSVFDAFGEIGTRKAPTPSLITGPTDFKDDPFKDYRYEDPFSIKDPFAEEGEEELSEGKGADHKRNFAEDFSSGDEIGSAAKTLSNIVNNNSIKPKAATPLNNDLLQQFDAFNLNSSPAPSHHSGTSYHSNSKPSHDSQTALDAFADFDDFAATLATTGGSGAATTATTTGNTKLNNSFFDAFNDNFSVIQSSSVPTGVVRPNDQRAKAFDAFNDNFEDQFKTSPNANFAKFDGFEAHNFSSDFGNASFGTTGKEKQNGQVDKKFQSKEPVKDKFAADYSKPESFDADLEEALKRSVLDN

Target ORF information:

RefSeq Version	NM_166687.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster epidermal growth factor receptor pathway substrate clone 15, transcript variant B (Eps-15), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166687.1

ORF Insert Sequence:

ATGAATGTGG ACTTTGCGAG GGTGTGCGGG AAGCACATAG GCGTCTACGA GGCCTACTAC 
AAACTGATTG ACCCCAAAGG CACAGGGGCA ATCGAGGCCA TGACTGCGGC CAAGTTTCTC 
AAGAAGTCGG GCCTTAGCGA CGTTGTGCTA AGCAGGATCT GGGACCTGTC TGATCCGAGC 
GGCAAGGGCT TTCTGGACAA GCCGGGCTTT TTTGTAGCCC TTAAGCTGGT GTCCCTGTCG 
CAGGCGGGAC AGGTGGCCAG CATGGCCAAC ATCTACCTGG ACATTGCCAA CCCGCCCAAG 
TTGGGTGAAC TACCAAAAAC GATGCCGTCG CGTATTCAGA CTGTTCCCGT TGCTAGCGGC 
GGAGTGGCAA ACGGAGACTG GTCCATCGGG GTGATCGATC GGCTCAAGTA CGAGCAGCTC 
TTCGAGTCCC TGCATCCCAG TAATGGAATG CTGCCGGGCA ACAAGGTTAA AGGCGTGCTC 
ATGGACTCCA AGCTGCCCAT GAGCATACTG GGAACTATAT GGGACCTGGC CGACCAGGAC 
AAGGACGGCA ACCTGGATAT GCACGAATTC GTGGTTGCCA TGCACCTGGT CTACCAGACG 
CTGCAGAAGC GCACCATTCC TAGCGTTCTG CCGCCCGAGC TGCGAAAGCC CGGAGGAGCA 
GGTCCGCCAC CGAAGCCAGC CATGCCACCA CCGCCAGCGG GTGCTGCTAT GCCACGTGCT 
CCCAGTGGGG AAGGATTTGG GGACGGCGGA TTTGTAGCAA ATTTTCCCAA GGATATAGCC 
CCGCCGGCGG CTATTCCGCC GCTCCCGGTG GCTGTGCCCC CCATGACGCG CATTCCCCCG 
GTGGGAGCAG TCAGCTCTCA GCCGCTGATT CAGACGGATC CATTGATACC GATTGGGGCT 
CCGGTGATGG CGAATGCCGA CTGGGTTGTC ACCCCAGCGG ACCTAAAGCG GTTCGAGGAG 
ATCTTCCGGC AATCGGATCT GGATAAGGAC GGCCTGGTCT CCGGCCTTGA GGTGAAGGAT 
ATTTTCATCA AGTCGGGTAT TCCGCAGCGA AGCCTAGCAG ACATCTGGGC TCTTTGTGAC 
ACCAATCAGT CCGGTAAGCT GACTGTGGAG CAGTTTGCAC TGGCCATGTG GTTCGTGGAG 
CGAAAGCAGC GCGGTGTGGA CCCGCCACAT GTCCTCAACG CCAATATGGT GCCGCCCTCA 
ATGCGCGCCA CTGTGGCTGG AGTCGATCTG CAGCCGCAGG AGGTCAAGCC CACTTACTCA 
AACCCGGAGC TGGAAATGAT CTCCAAGGAG ATTGAGGAGT TGGCCCGTGA GCGGCGGGTT 
CTGGAGACGG AGATTGCCCA AAAGGAAGCG GATGTGCGCA TCAAAAACGG CGAAGTGCGC 
AGTTTGCAGA GTGAACTGGA TACTCTTACC GCCACACTGA AGCAGCTGGA GAACCAGCGA 
GGAGAGGCCC AGAAGCGACT GGATGACCTG CAGGCTCAAG TGACCAAGAT TCGCGACCAG 
TGCCACATGC AGGAGGTGAC CATCAACGAG CAGGAGGGCG AATTAAACGC AAAACGCTCG 
GAGCTGCAGA AGCTCAAGGA CGAAGAAGCT TCGTTGCAAA AGGAGTACGA CAGCAACAAT 
CGGGAGTTAT CCAAGCTAAC CAACCACCTG CAGGCAACCC AACTGCAAAT CAGCTCGGTC 
CGATCAATGG TCACACAGCT GCTGGAGACG CAGCGACAGA TGACCGACGC TTTGCTCATT 
TGTCGGGCTG CCATGGAGAA CCAAAACGCG GAACTCGTAT CCGAATACCA GTTGAAGATA 
GAGCCGGACT TTGATGAGGC GCGCAAGACC CTGACAAAGG AAGTGCAACT GCCCAAGGAT 
GATCCCTTTG AGGAGAACAA CAGCGGCGCC GCCAATCAAG CTACCAACGG CTTTGGCAGC 
GATCCCTTCT CCGGTCAGCC GGTCAACAAG CCGGCTATTT CTACAGGCTT TGATGACAGC 
TTCAACATGA GCTCAGGCTT CGATAGTGGC TTCGATGCCT TTGGTCAGAG CGGAGCGGGC 
TCAGCCTTTG GTCAGACCCA GCGGGATCCA TTCGGCTCGG ATGCCTTTGC AGCCAACAAG 
AGCAACGCCA TTACTCCTGA GCCCGGCAAG GACGACTTCG GCAGTGATCC GTTTGCTGCC 
TTGCATGCTC CAACCGGCCA GGGTCAGGTG CTCAGTCCCA ACGCCCAGAA GTCCGGACCG 
CCGCCCAGAC CGGAGTCTCC TAGTCCAGCA TTGCCGCCAA AGAAGTCCAA GGTGCCGCCC 
CCACGACCAG CGCCACCACG AGCAGCCCAG CCCACGGGTG GTTTCGGAAG CGGCGGCGGA 
GGAGGATTTG CCGACTTTGA CGACTTCGAC AATAAGCTCC ACCACATTCC AAGCGCACCC 
TCGCCCTCGG CCACTGCACT GTCCCCGCTC CCACCCACGC TCCCAGCCCC CATTCCGGTC 
GTCAGTGGAT CGTCATTGCT AGACAGCTTC ACGCTATTCG ACGATCCCGG CCAAATCCAC 
AAGCAGGCGG CAAGCACGCC CAATCCCACG CCCATCACTG TCCCCACCGT ACACACCCTG 
CTCCAGACAT CTTTATCCAC GCCAGCAGCC CCATCCCCGG CATTGGCTTC TCTATCAACA 
TCAGGATCGG GATCGGTAGC GGGAGCGGGG GCAGACCTCC CCAGTAGCGT CACCATTACC 
ACCGCCCCCA GCTTGAATAA CCAGCATCTG TCGCGTTCCA ATACACCGCT GCAGAACCAG 
AGGACGGCGG ATGTGAAATT GGAGACCAAG GCGGTAGTCA GTGTGTTTGA TGCATTTGGA 
GAGATTGGAA CTCGGAAGGC CCCGACTCCC AGTCTCATTA CAGGGCCAAC CGACTTCAAG 
GATGACCCCT TCAAGGATTA CCGTTACGAA GATCCATTTA GCATCAAGGA TCCTTTTGCC 
GAAGAGGGCG AGGAGGAGTT GTCAGAAGGC AAGGGTGCTG ACCACAAGAG GAACTTTGCG 
GAGGACTTTA GCTCGGGTGA TGAGATTGGA TCAGCCGCCA AAACTCTGAG TAACATCGTG 
AACAATAATA GCATTAAGCC CAAGGCGGCC ACGCCGCTAA ACAACGACCT GCTGCAGCAG 
TTCGACGCCT TCAACTTGAA CTCGAGTCCG GCGCCGTCGC ACCACTCAGG CACTTCGTAC 
CACTCCAACT CCAAGCCGAG CCACGACTCG CAGACGGCGC TGGACGCATT CGCTGACTTC 
GACGACTTCG CAGCTACGCT GGCAACCACT GGTGGATCTG GGGCTGCAAC CACGGCCACA 
ACCACTGGCA ATACGAAACT CAACAATTCC TTTTTTGATG CATTTAACGA CAACTTCAGT 
GTGATCCAGA GCTCATCAGT ACCCACTGGC GTGGTCAGGC CCAACGATCA AAGGGCAAAG 
GCATTTGACG CCTTCAACGA CAATTTCGAG GACCAATTCA AGACAAGCCC CAACGCCAAC 
TTCGCCAAGT TCGATGGCTT CGAGGCACAC AACTTCTCGA GTGACTTCGG AAACGCCTCC 
TTCGGAACGA CGGGCAAGGA GAAGCAGAAC GGGCAGGTGG ACAAGAAGTT TCAGAGCAAG 
GAGCCGGTCA AGGACAAGTT TGCTGCAGAC TATTCCAAGC CGGAGAGCTT CGACGCGGAC 
CTGGAGGAGG CGCTGAAGCG CAGCGTACTG GACAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eps-15 ( NM_138121.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611965.2 Drosophila melanogaster epidermal growth factor receptor pathway substrate clone 15, transcript variant A (Eps-15), mRNA.

Price & Availability↑

CloneID	OFa11234
Clone ID Related Accession (Same CDS sequence)	NM_138121.2
Accession Version	NM_138121.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	epidermal growth factor receptor pathway substrate clone 15, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 31, 2002 this sequence version replaced NM_138121.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATGTGG ACTTTGCGAG GGTGTGCGGG AAGCACATAG GCGTCTACGA GGCCTACTAC 
AAACTGATTG ACCCCAAAGG CACAGGGGCA ATCGAGGCCA TGACTGCGGC CAAGTTTCTC 
AAGAAGTCGG GCCTTAGCGA CGTTGTGCTA AGCAGGATCT GGGACCTGTC TGATCCGAGC 
GGCAAGGGCT TTCTGGACAA GCCGGGCTTT TTTGTAGCCC TTAAGCTGGT GTCCCTGTCG 
CAGGCGGGAC AGGTGGCCAG CATGGCCAAC ATCTACCTGG ACATTGCCAA CCCGCCCAAG 
TTGGGTGAAC TACCAAAAAC GATGCCGTCG CGTATTCAGA CTGTTCCCGT TGCTAGCGGC 
GGAGTGGCAA ACGGAGACTG GTCCATCGGG GTGATCGATC GGCTCAAGTA CGAGCAGCTC 
TTCGAGTCCC TGCATCCCAG TAATGGAATG CTGCCGGGCA ACAAGGTTAA AGGCGTGCTC 
ATGGACTCCA AGCTGCCCAT GAGCATACTG GGAACTATAT GGGACCTGGC CGACCAGGAC 
AAGGACGGCA ACCTGGATAT GCACGAATTC GTGGTTGCCA TGCACCTGGT CTACCAGACG 
CTGCAGAAGC GCACCATTCC TAGCGTTCTG CCGCCCGAGC TGCGAAAGCC CGGAGGAGCA 
GGTCCGCCAC CGAAGCCAGC CATGCCACCA CCGCCAGCGG GTGCTGCTAT GCCACGTGCT 
CCCAGTGGGG AAGGATTTGG GGACGGCGGA TTTGTAGCAA ATTTTCCCAA GGATATAGCC 
CCGCCGGCGG CTATTCCGCC GCTCCCGGTG GCTGTGCCCC CCATGACGCG CATTCCCCCG 
GTGGGAGCAG TCAGCTCTCA GCCGCTGATT CAGACGGATC CATTGATACC GATTGGGGCT 
CCGGTGATGG CGAATGCCGA CTGGGTTGTC ACCCCAGCGG ACCTAAAGCG GTTCGAGGAG 
ATCTTCCGGC AATCGGATCT GGATAAGGAC GGCCTGGTCT CCGGCCTTGA GGTGAAGGAT 
ATTTTCATCA AGTCGGGTAT TCCGCAGCGA AGCCTAGCAG ACATCTGGGC TCTTTGTGAC 
ACCAATCAGT CCGGTAAGCT GACTGTGGAG CAGTTTGCAC TGGCCATGTG GTTCGTGGAG 
CGAAAGCAGC GCGGTGTGGA CCCGCCACAT GTCCTCAACG CCAATATGGT GCCGCCCTCA 
ATGCGCGCCA CTGTGGCTGG AGTCGATCTG CAGCCGCAGG AGGTCAAGCC CACTTACTCA 
AACCCGGAGC TGGAAATGAT CTCCAAGGAG ATTGAGGAGT TGGCCCGTGA GCGGCGGGTT 
CTGGAGACGG AGATTGCCCA AAAGGAAGCG GATGTGCGCA TCAAAAACGG CGAAGTGCGC 
AGTTTGCAGA GTGAACTGGA TACTCTTACC GCCACACTGA AGCAGCTGGA GAACCAGCGA 
GGAGAGGCCC AGAAGCGACT GGATGACCTG CAGGCTCAAG TTAGCCACAA TACGGCCGTG 
CTGGCCAACG TAAGTCTTGA CATATCGCGC ACCAACGAGC AGGTGACCAA GATTCGCGAC 
CAGTGCCACA TGCAGGAGGT GACCATCAAC GAGCAGGAGG GCGAATTAAA CGCAAAACGC 
TCGGAGCTGC AGAAGCTCAA GGACGAAGAA GCTTCGTTGC AAAAGGAGTA CGACAGCAAC 
AATCGGGAGT TATCCAAGCT AACCAACCAC CTGCAGGCAA CCCAACTGCA AATCAGCTCG 
GTCCGATCAA TGGTCACACA GCTGCTGGAG ACGCAGCGAC AGATGACCGA CGCTTTGCTC 
ATTTGTCGGG CTGCCATGGA GAACCAAAAC GCGGAACTCG TATCCGAATA CCAGTTGAAG 
ATAGAGCCGG ACTTTGATGA GGCGCGCAAG ACCCTGACAA AGGAAGTGCA ACTGCCCAAG 
GATGATCCCT TTGAGGAGAA CAACAGCGGC GCCGCCAATC AAGCTACCAA CGGCTTTGGC 
AGCGATCCCT TCTCCGGTCA GCCGGTCAAC AAGCCGGCTA TTTCTACAGG CTTTGATGAC 
AGCTTCAACA TGAGCTCAGG CTTCGATAGT GGCTTCGATG CCTTTGGTCA GAGCGGAGCG 
GGCTCAGCCT TTGGTCAGAC CCAGCGGGAT CCATTCGGCT CGGATGCCTT TGCAGCCAAC 
AAGAGCAACG CCATTACTCC TGAGCCCGGC AAGGACGACT TCGGCAGTGA TCCGTTTGCT 
GCCTTGCATG CTCCAACCGG CCAGGGTCAG GTGCTCAGTC CCAACGCCCA GAAGTCCGGA 
CCGCCGCCCA GACCGGAGTC TCCTAGTCCA GCATTGCCGC CAAAGAAGTC CAAGGTGCCG 
CCCCCACGAC CAGCGCCACC ACGAGCAGCC CAGCCCACGG GTGGTTTCGG AAGCGGCGGC 
GGAGGAGGAT TTGCCGACTT TGACGACTTC GACAATAAGC TCCACCACAT TCCAAGCGCA 
CCCTCGCCCT CGGCCACTGC ACTGTCCCCG CTCCCACCCA CGCTCCCAGC CCCCATTCCG 
GTCGTCAGTG GATCGTCATT GCTAGACAGC TTCACGCTAT TCGACGATCC CGGCCAAATC 
CACAAGCAGG CGGCAAGCAC GCCCAATCCC ACGCCCATCA CTGTCCCCAC CGTACACACC 
CTGCTCCAGA CATCTTTATC CACGCCAGCA GCCCCATCCC CGGCATTGGC TTCTCTATCA 
ACATCAGGAT CGGGATCGGT AGCGGGAGCG GGGGCAGACC TCCCCAGTAG CGTCACCATT 
ACCACCGCCC CCAGCTTGAA TAACCAGCAT CTGTCGCGTT CCAATACACC GCTGCAGAAC 
CAGAGGACGG CGGATGTGAA ATTGGAGACC AAGGCGGTAG TCAGTGTGTT TGATGCATTT 
GGAGAGATTG GAACTCGGAA GGCCCCGACT CCCAGTCTCA TTACAGGGCC AACCGACTTC 
AAGGATGACC CCTTCAAGGA TTACCGTTAC GAAGATCCAT TTAGCATCAA GGATCCTTTT 
GCCGAAGAGG GCGAGGAGGA GTTGTCAGAA GGCAAGGGTG CTGACCACAA GAGGAACTTT 
GCGGAGGACT TTAGCTCGGG TGATGAGATT GGATCAGCCG CCAAAACTCT GAGTAACATC 
GTGAACAATA ATAGCATTAA GCCCAAGGCG GCCACGCCGC TAAACAACGA CCTGCTGCAG 
CAGTTCGACG CCTTCAACTT GAACTCGAGT CCGGCGCCGT CGCACCACTC AGGCACTTCG 
TACCACTCCA ACTCCAAGCC GAGCCACGAC TCGCAGACGG CGCTGGACGC ATTCGCTGAC 
TTCGACGACT TCGCAGCTAC GCTGGCAACC ACTGGTGGAT CTGGGGCTGC AACCACGGCC 
ACAACCACTG GCAATACGAA ACTCAACAAT TCCTTTTTTG ATGCATTTAA CGACAACTTC 
AGTGTGATCC AGAGCTCATC AGTACCCACT GGCGTGGTCA GGCCCAACGA TCAAAGGGCA 
AAGGCATTTG ACGCCTTCAA CGACAATTTC GAGGACCAAT TCAAGACAAG CCCCAACGCC 
AACTTCGCCA AGTTCGATGG CTTCGAGGCA CACAACTTCT CGAGTGACTT CGGAAACGCC 
TCCTTCGGAA CGACGGGCAA GGAGAAGCAG AACGGGCAGG TGGACAAGAA GTTTCAGAGC 
AAGGAGCCGG TCAAGGACAA GTTTGCTGCA GACTATTCCA AGCCGGAGAG CTTCGACGCG 
GACCTGGAGG AGGCGCTGAA GCGCAGCGTA CTGGACAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611965.2
CDS	293..4054
Translation	MNVDFARVCGKHIGVYEAYYKLIDPKGTGAIEAMTAAKFLKKSGLSDVVLSRIWDLSDPSGKGFLDKPGFFVALKLVSLSQAGQVASMANIYLDIANPPKLGELPKTMPSRIQTVPVASGGVANGDWSIGVIDRLKYEQLFESLHPSNGMLPGNKVKGVLMDSKLPMSILGTIWDLADQDKDGNLDMHEFVVAMHLVYQTLQKRTIPSVLPPELRKPGGAGPPPKPAMPPPPAGAAMPRAPSGEGFGDGGFVANFPKDIAPPAAIPPLPVAVPPMTRIPPVGAVSSQPLIQTDPLIPIGAPVMANADWVVTPADLKRFEEIFRQSDLDKDGLVSGLEVKDIFIKSGIPQRSLADIWALCDTNQSGKLTVEQFALAMWFVERKQRGVDPPHVLNANMVPPSMRATVAGVDLQPQEVKPTYSNPELEMISKEIEELARERRVLETEIAQKEADVRIKNGEVRSLQSELDTLTATLKQLENQRGEAQKRLDDLQAQVSHNTAVLANVSLDISRTNEQVTKIRDQCHMQEVTINEQEGELNAKRSELQKLKDEEASLQKEYDSNNRELSKLTNHLQATQLQISSVRSMVTQLLETQRQMTDALLICRAAMENQNAELVSEYQLKIEPDFDEARKTLTKEVQLPKDDPFEENNSGAANQATNGFGSDPFSGQPVNKPAISTGFDDSFNMSSGFDSGFDAFGQSGAGSAFGQTQRDPFGSDAFAANKSNAITPEPGKDDFGSDPFAALHAPTGQGQVLSPNAQKSGPPPRPESPSPALPPKKSKVPPPRPAPPRAAQPTGGFGSGGGGGFADFDDFDNKLHHIPSAPSPSATALSPLPPTLPAPIPVVSGSSLLDSFTLFDDPGQIHKQAASTPNPTPITVPTVHTLLQTSLSTPAAPSPALASLSTSGSGSVAGAGADLPSSVTITTAPSLNNQHLSRSNTPLQNQRTADVKLETKAVVSVFDAFGEIGTRKAPTPSLITGPTDFKDDPFKDYRYEDPFSIKDPFAEEGEEELSEGKGADHKRNFAEDFSSGDEIGSAAKTLSNIVNNNSIKPKAATPLNNDLLQQFDAFNLNSSPAPSHHSGTSYHSNSKPSHDSQTALDAFADFDDFAATLATTGGSGAATTATTTGNTKLNNSFFDAFNDNFSVIQSSSVPTGVVRPNDQRAKAFDAFNDNFEDQFKTSPNANFAKFDGFEAHNFSSDFGNASFGTTGKEKQNGQVDKKFQSKEPVKDKFAADYSKPESFDADLEEALKRSVLDN

Target ORF information:

RefSeq Version	NM_138121.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster epidermal growth factor receptor pathway substrate clone 15, transcript variant A (Eps-15), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138121.2

ORF Insert Sequence:

ATGAATGTGG ACTTTGCGAG GGTGTGCGGG AAGCACATAG GCGTCTACGA GGCCTACTAC 
AAACTGATTG ACCCCAAAGG CACAGGGGCA ATCGAGGCCA TGACTGCGGC CAAGTTTCTC 
AAGAAGTCGG GCCTTAGCGA CGTTGTGCTA AGCAGGATCT GGGACCTGTC TGATCCGAGC 
GGCAAGGGCT TTCTGGACAA GCCGGGCTTT TTTGTAGCCC TTAAGCTGGT GTCCCTGTCG 
CAGGCGGGAC AGGTGGCCAG CATGGCCAAC ATCTACCTGG ACATTGCCAA CCCGCCCAAG 
TTGGGTGAAC TACCAAAAAC GATGCCGTCG CGTATTCAGA CTGTTCCCGT TGCTAGCGGC 
GGAGTGGCAA ACGGAGACTG GTCCATCGGG GTGATCGATC GGCTCAAGTA CGAGCAGCTC 
TTCGAGTCCC TGCATCCCAG TAATGGAATG CTGCCGGGCA ACAAGGTTAA AGGCGTGCTC 
ATGGACTCCA AGCTGCCCAT GAGCATACTG GGAACTATAT GGGACCTGGC CGACCAGGAC 
AAGGACGGCA ACCTGGATAT GCACGAATTC GTGGTTGCCA TGCACCTGGT CTACCAGACG 
CTGCAGAAGC GCACCATTCC TAGCGTTCTG CCGCCCGAGC TGCGAAAGCC CGGAGGAGCA 
GGTCCGCCAC CGAAGCCAGC CATGCCACCA CCGCCAGCGG GTGCTGCTAT GCCACGTGCT 
CCCAGTGGGG AAGGATTTGG GGACGGCGGA TTTGTAGCAA ATTTTCCCAA GGATATAGCC 
CCGCCGGCGG CTATTCCGCC GCTCCCGGTG GCTGTGCCCC CCATGACGCG CATTCCCCCG 
GTGGGAGCAG TCAGCTCTCA GCCGCTGATT CAGACGGATC CATTGATACC GATTGGGGCT 
CCGGTGATGG CGAATGCCGA CTGGGTTGTC ACCCCAGCGG ACCTAAAGCG GTTCGAGGAG 
ATCTTCCGGC AATCGGATCT GGATAAGGAC GGCCTGGTCT CCGGCCTTGA GGTGAAGGAT 
ATTTTCATCA AGTCGGGTAT TCCGCAGCGA AGCCTAGCAG ACATCTGGGC TCTTTGTGAC 
ACCAATCAGT CCGGTAAGCT GACTGTGGAG CAGTTTGCAC TGGCCATGTG GTTCGTGGAG 
CGAAAGCAGC GCGGTGTGGA CCCGCCACAT GTCCTCAACG CCAATATGGT GCCGCCCTCA 
ATGCGCGCCA CTGTGGCTGG AGTCGATCTG CAGCCGCAGG AGGTCAAGCC CACTTACTCA 
AACCCGGAGC TGGAAATGAT CTCCAAGGAG ATTGAGGAGT TGGCCCGTGA GCGGCGGGTT 
CTGGAGACGG AGATTGCCCA AAAGGAAGCG GATGTGCGCA TCAAAAACGG CGAAGTGCGC 
AGTTTGCAGA GTGAACTGGA TACTCTTACC GCCACACTGA AGCAGCTGGA GAACCAGCGA 
GGAGAGGCCC AGAAGCGACT GGATGACCTG CAGGCTCAAG TTAGCCACAA TACGGCCGTG 
CTGGCCAACG TAAGTCTTGA CATATCGCGC ACCAACGAGC AGGTGACCAA GATTCGCGAC 
CAGTGCCACA TGCAGGAGGT GACCATCAAC GAGCAGGAGG GCGAATTAAA CGCAAAACGC 
TCGGAGCTGC AGAAGCTCAA GGACGAAGAA GCTTCGTTGC AAAAGGAGTA CGACAGCAAC 
AATCGGGAGT TATCCAAGCT AACCAACCAC CTGCAGGCAA CCCAACTGCA AATCAGCTCG 
GTCCGATCAA TGGTCACACA GCTGCTGGAG ACGCAGCGAC AGATGACCGA CGCTTTGCTC 
ATTTGTCGGG CTGCCATGGA GAACCAAAAC GCGGAACTCG TATCCGAATA CCAGTTGAAG 
ATAGAGCCGG ACTTTGATGA GGCGCGCAAG ACCCTGACAA AGGAAGTGCA ACTGCCCAAG 
GATGATCCCT TTGAGGAGAA CAACAGCGGC GCCGCCAATC AAGCTACCAA CGGCTTTGGC 
AGCGATCCCT TCTCCGGTCA GCCGGTCAAC AAGCCGGCTA TTTCTACAGG CTTTGATGAC 
AGCTTCAACA TGAGCTCAGG CTTCGATAGT GGCTTCGATG CCTTTGGTCA GAGCGGAGCG 
GGCTCAGCCT TTGGTCAGAC CCAGCGGGAT CCATTCGGCT CGGATGCCTT TGCAGCCAAC 
AAGAGCAACG CCATTACTCC TGAGCCCGGC AAGGACGACT TCGGCAGTGA TCCGTTTGCT 
GCCTTGCATG CTCCAACCGG CCAGGGTCAG GTGCTCAGTC CCAACGCCCA GAAGTCCGGA 
CCGCCGCCCA GACCGGAGTC TCCTAGTCCA GCATTGCCGC CAAAGAAGTC CAAGGTGCCG 
CCCCCACGAC CAGCGCCACC ACGAGCAGCC CAGCCCACGG GTGGTTTCGG AAGCGGCGGC 
GGAGGAGGAT TTGCCGACTT TGACGACTTC GACAATAAGC TCCACCACAT TCCAAGCGCA 
CCCTCGCCCT CGGCCACTGC ACTGTCCCCG CTCCCACCCA CGCTCCCAGC CCCCATTCCG 
GTCGTCAGTG GATCGTCATT GCTAGACAGC TTCACGCTAT TCGACGATCC CGGCCAAATC 
CACAAGCAGG CGGCAAGCAC GCCCAATCCC ACGCCCATCA CTGTCCCCAC CGTACACACC 
CTGCTCCAGA CATCTTTATC CACGCCAGCA GCCCCATCCC CGGCATTGGC TTCTCTATCA 
ACATCAGGAT CGGGATCGGT AGCGGGAGCG GGGGCAGACC TCCCCAGTAG CGTCACCATT 
ACCACCGCCC CCAGCTTGAA TAACCAGCAT CTGTCGCGTT CCAATACACC GCTGCAGAAC 
CAGAGGACGG CGGATGTGAA ATTGGAGACC AAGGCGGTAG TCAGTGTGTT TGATGCATTT 
GGAGAGATTG GAACTCGGAA GGCCCCGACT CCCAGTCTCA TTACAGGGCC AACCGACTTC 
AAGGATGACC CCTTCAAGGA TTACCGTTAC GAAGATCCAT TTAGCATCAA GGATCCTTTT 
GCCGAAGAGG GCGAGGAGGA GTTGTCAGAA GGCAAGGGTG CTGACCACAA GAGGAACTTT 
GCGGAGGACT TTAGCTCGGG TGATGAGATT GGATCAGCCG CCAAAACTCT GAGTAACATC 
GTGAACAATA ATAGCATTAA GCCCAAGGCG GCCACGCCGC TAAACAACGA CCTGCTGCAG 
CAGTTCGACG CCTTCAACTT GAACTCGAGT CCGGCGCCGT CGCACCACTC AGGCACTTCG 
TACCACTCCA ACTCCAAGCC GAGCCACGAC TCGCAGACGG CGCTGGACGC ATTCGCTGAC 
TTCGACGACT TCGCAGCTAC GCTGGCAACC ACTGGTGGAT CTGGGGCTGC AACCACGGCC 
ACAACCACTG GCAATACGAA ACTCAACAAT TCCTTTTTTG ATGCATTTAA CGACAACTTC 
AGTGTGATCC AGAGCTCATC AGTACCCACT GGCGTGGTCA GGCCCAACGA TCAAAGGGCA 
AAGGCATTTG ACGCCTTCAA CGACAATTTC GAGGACCAAT TCAAGACAAG CCCCAACGCC 
AACTTCGCCA AGTTCGATGG CTTCGAGGCA CACAACTTCT CGAGTGACTT CGGAAACGCC 
TCCTTCGGAA CGACGGGCAA GGAGAAGCAG AACGGGCAGG TGGACAAGAA GTTTCAGAGC 
AAGGAGCCGG TCAAGGACAA GTTTGCTGCA GACTATTCCA AGCCGGAGAG CTTCGACGCG 
GACCTGGAGG AGGCGCTGAA GCGCAGCGTA CTGGACAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Eps-15 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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