pio cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	pio
Entrez Gene ID	37929
Full Name	piopio
Synonyms	CG30167,CG3541,Dmel\CG3541,Pio
General protein information	Preferred Names piopio Names CG3541 gene product from transcript CG3541-RD CG3541-PB CG3541-PC CG3541-PD CG3541-PE pio-PB pio-PC pio-PD pio-PE
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 60D7-60D8

mRNA and Protein(s)

mRNA	Protein	Name
NM_138094.4	NP_611938.2	piopio, isoform C
NM_166677.3	NP_726461.1	piopio, isoform B
NM_001299920.1	NP_001286849.1	piopio, isoform E
NM_001169824.2	NP_001163295.1	piopio, isoform D

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007051	XP_308712.4
Drosophila melanogaster (fruit fly)	pio	NP_001163295.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pio gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pio cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa11200	NM_001169824.2 Latest version!	Drosophila melanogaster piopio (pio), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa11200	NM_001299920.1 Latest version!	Drosophila melanogaster piopio (pio), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa11200	NM_166677.3 Latest version!	Drosophila melanogaster piopio (pio), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa11201	NM_138094.4 Latest version!	Drosophila melanogaster piopio (pio), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pio ( NM_166677.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726461.1 Drosophila melanogaster piopio (pio), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa11200
Clone ID Related Accession (Same CDS sequence)	NM_166677.3 , NM_001299920.1 , NM_001169824.2
Accession Version	NM_166677.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	piopio, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166677.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGACAG GCACTCGAAT GGACGCTTTC CACACGGCGC TGCACTTAAT CACAATCGCA 
GCTCTGACGA CGCACGCGGC GCAGATTCCA ACGGCAATGA AAGATGCCCA GAGCTCGCTG 
AGCGATGCGA TTGCGGCGGC GGAGGCGGAG GTGGCCTCCA CCTCTAAGCC GGCGGTGGAG 
CCGAGTGTGC GGATCAAGTG CCTCTCCGGA TCGATGCTGA TCACCATTAA AGACGCTCCT 
CCGAACCACG AAACGGGCCT GTTCAGCGGC ATGATCTACC CGAAAGGCCT GTCCAAGAAC 
TCCACCTGCT TGAGCGAGTA CAGAGATCAT GTGGGCTCCC TGCGATATAA GCTGCCCCTG 
AGATCTTGCA ACACGATGCC GAAGGAAACG GATGACGGTG GCATTGAGTT CTTCAACACC 
ATCGTGCTGC AGCCCCACCT AAAGCTCATC ACGGACCTGG GACGTGGCTA CCACGTCCGC 
TGTGCGTACA AGAGTCGGGA CGCAGCCATG AAGCCCAAAA AGTACCTGCG CAAGCACGCC 
CAGAAGCCAC AGGCCTTCCG CAGCGATGAT CGGCGGGAGT ACGGACGATC GCTGGACAAG 
CAGCAGGACG ACGACCTGGA CGAGGAGGAT GTCTACGATG CGAATGCCCC GACGCAGGAG 
GAGGACGTGA CCAACAACGA GATCCCCATG CCAGGCTGCC ACATGAAGAT CTACAACGAC 
GAGCACAAAA TTGCCGACGA TGTGAAGATC GGTGATCCTC TGACCATTGT CATCAGCATC 
GATAAGCAGA AGGTTTACGG ACTCCATGTC ACGGACTGCA TAGTTCGCGA TGGTCTGGGC 
TGGGGAGAGC AGCGACTGGT GGGCGAAGAT GGCTGCCCCA TGGACAACGA GATCATGGGT 
CAGTTCAACT ACACCCAAGA CCGATTGGCA GCCAACGTGA CCTTTCCGGC GCACAAGTTC 
CCCTACACCA CCTCCGTGTA CTACCAGTGC AACGTGCGAC TGTGCGCCCT GGAGGACCCC 
ACCTGCCAGG AGGCTCCCCA GTGCAGCGGC AAGAGGCCCA AGCGCCAAGC CGCCGCGGAC 
AGCAAGGAGG AGGACGGTCT GCCCGCCACC ATTGAGGTCT TCTCTGGCCT ATATGTGAAT 
GAGAACGAAA ACGCCAACGA CAGCGACGAG GACGCGGTTT ACAAGGAGAA GACTCTGGAC 
GACGCCCTGT GCGTTTCGCA ACGCACCTTC GCCATAGCCA TTGCCATCGC CGGATTGATC 
CTTATGCTAG CCGTGGTCGC CGCCGTTCTC TGCATCATGG CGCGCAGGAG CACCAAGACG 
GTGTCCAACT CTGGCAGCTC CATCTACAGT GGACCCTACA CGAACACCGC CTTCTCGCAC 
AGCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726461.1
CDS	371..1759
Translation	MKTGTRMDAFHTALHLITIAALTTHAAQIPTAMKDAQSSLSDAIAAAEAEVASTSKPAVEPSVRIKCLSGSMLITIKDAPPNHETGLFSGMIYPKGLSKNSTCLSEYRDHVGSLRYKLPLRSCNTMPKETDDGGIEFFNTIVLQPHLKLITDLGRGYHVRCAYKSRDAAMKPKKYLRKHAQKPQAFRSDDRREYGRSLDKQQDDDLDEEDVYDANAPTQEEDVTNNEIPMPGCHMKIYNDEHKIADDVKIGDPLTIVISIDKQKVYGLHVTDCIVRDGLGWGEQRLVGEDGCPMDNEIMGQFNYTQDRLAANVTFPAHKFPYTTSVYYQCNVRLCALEDPTCQEAPQCSGKRPKRQAAADSKEEDGLPATIEVFSGLYVNENENANDSDEDAVYKEKTLDDALCVSQRTFAIAIAIAGLILMLAVVAAVLCIMARRSTKTVSNSGSSIYSGPYTNTAFSHSS

Target ORF information:

RefSeq Version	NM_166677.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster piopio (pio), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166677.3

ORF Insert Sequence:

ATGAAGACAG GCACTCGAAT GGACGCTTTC CACACGGCGC TGCACTTAAT CACAATCGCA 
GCTCTGACGA CGCACGCGGC GCAGATTCCA ACGGCAATGA AAGATGCCCA GAGCTCGCTG 
AGCGATGCGA TTGCGGCGGC GGAGGCGGAG GTGGCCTCCA CCTCTAAGCC GGCGGTGGAG 
CCGAGTGTGC GGATCAAGTG CCTCTCCGGA TCGATGCTGA TCACCATTAA AGACGCTCCT 
CCGAACCACG AAACGGGCCT GTTCAGCGGC ATGATCTACC CGAAAGGCCT GTCCAAGAAC 
TCCACCTGCT TGAGCGAGTA CAGAGATCAT GTGGGCTCCC TGCGATATAA GCTGCCCCTG 
AGATCTTGCA ACACGATGCC GAAGGAAACG GATGACGGTG GCATTGAGTT CTTCAACACC 
ATCGTGCTGC AGCCCCACCT AAAGCTCATC ACGGACCTGG GACGTGGCTA CCACGTCCGC 
TGTGCGTACA AGAGTCGGGA CGCAGCCATG AAGCCCAAAA AGTACCTGCG CAAGCACGCC 
CAGAAGCCAC AGGCCTTCCG CAGCGATGAT CGGCGGGAGT ACGGACGATC GCTGGACAAG 
CAGCAGGACG ACGACCTGGA CGAGGAGGAT GTCTACGATG CGAATGCCCC GACGCAGGAG 
GAGGACGTGA CCAACAACGA GATCCCCATG CCAGGCTGCC ACATGAAGAT CTACAACGAC 
GAGCACAAAA TTGCCGACGA TGTGAAGATC GGTGATCCTC TGACCATTGT CATCAGCATC 
GATAAGCAGA AGGTTTACGG ACTCCATGTC ACGGACTGCA TAGTTCGCGA TGGTCTGGGC 
TGGGGAGAGC AGCGACTGGT GGGCGAAGAT GGCTGCCCCA TGGACAACGA GATCATGGGT 
CAGTTCAACT ACACCCAAGA CCGATTGGCA GCCAACGTGA CCTTTCCGGC GCACAAGTTC 
CCCTACACCA CCTCCGTGTA CTACCAGTGC AACGTGCGAC TGTGCGCCCT GGAGGACCCC 
ACCTGCCAGG AGGCTCCCCA GTGCAGCGGC AAGAGGCCCA AGCGCCAAGC CGCCGCGGAC 
AGCAAGGAGG AGGACGGTCT GCCCGCCACC ATTGAGGTCT TCTCTGGCCT ATATGTGAAT 
GAGAACGAAA ACGCCAACGA CAGCGACGAG GACGCGGTTT ACAAGGAGAA GACTCTGGAC 
GACGCCCTGT GCGTTTCGCA ACGCACCTTC GCCATAGCCA TTGCCATCGC CGGATTGATC 
CTTATGCTAG CCGTGGTCGC CGCCGTTCTC TGCATCATGG CGCGCAGGAG CACCAAGACG 
GTGTCCAACT CTGGCAGCTC CATCTACAGT GGACCCTACA CGAACACCGC CTTCTCGCAC 
AGCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pio ( NM_001299920.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286849.1 Drosophila melanogaster piopio (pio), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa11200
Clone ID Related Accession (Same CDS sequence)	NM_166677.3 , NM_001299920.1 , NM_001169824.2
Accession Version	NM_001299920.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	piopio, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGACAG GCACTCGAAT GGACGCTTTC CACACGGCGC TGCACTTAAT CACAATCGCA 
GCTCTGACGA CGCACGCGGC GCAGATTCCA ACGGCAATGA AAGATGCCCA GAGCTCGCTG 
AGCGATGCGA TTGCGGCGGC GGAGGCGGAG GTGGCCTCCA CCTCTAAGCC GGCGGTGGAG 
CCGAGTGTGC GGATCAAGTG CCTCTCCGGA TCGATGCTGA TCACCATTAA AGACGCTCCT 
CCGAACCACG AAACGGGCCT GTTCAGCGGC ATGATCTACC CGAAAGGCCT GTCCAAGAAC 
TCCACCTGCT TGAGCGAGTA CAGAGATCAT GTGGGCTCCC TGCGATATAA GCTGCCCCTG 
AGATCTTGCA ACACGATGCC GAAGGAAACG GATGACGGTG GCATTGAGTT CTTCAACACC 
ATCGTGCTGC AGCCCCACCT AAAGCTCATC ACGGACCTGG GACGTGGCTA CCACGTCCGC 
TGTGCGTACA AGAGTCGGGA CGCAGCCATG AAGCCCAAAA AGTACCTGCG CAAGCACGCC 
CAGAAGCCAC AGGCCTTCCG CAGCGATGAT CGGCGGGAGT ACGGACGATC GCTGGACAAG 
CAGCAGGACG ACGACCTGGA CGAGGAGGAT GTCTACGATG CGAATGCCCC GACGCAGGAG 
GAGGACGTGA CCAACAACGA GATCCCCATG CCAGGCTGCC ACATGAAGAT CTACAACGAC 
GAGCACAAAA TTGCCGACGA TGTGAAGATC GGTGATCCTC TGACCATTGT CATCAGCATC 
GATAAGCAGA AGGTTTACGG ACTCCATGTC ACGGACTGCA TAGTTCGCGA TGGTCTGGGC 
TGGGGAGAGC AGCGACTGGT GGGCGAAGAT GGCTGCCCCA TGGACAACGA GATCATGGGT 
CAGTTCAACT ACACCCAAGA CCGATTGGCA GCCAACGTGA CCTTTCCGGC GCACAAGTTC 
CCCTACACCA CCTCCGTGTA CTACCAGTGC AACGTGCGAC TGTGCGCCCT GGAGGACCCC 
ACCTGCCAGG AGGCTCCCCA GTGCAGCGGC AAGAGGCCCA AGCGCCAAGC CGCCGCGGAC 
AGCAAGGAGG AGGACGGTCT GCCCGCCACC ATTGAGGTCT TCTCTGGCCT ATATGTGAAT 
GAGAACGAAA ACGCCAACGA CAGCGACGAG GACGCGGTTT ACAAGGAGAA GACTCTGGAC 
GACGCCCTGT GCGTTTCGCA ACGCACCTTC GCCATAGCCA TTGCCATCGC CGGATTGATC 
CTTATGCTAG CCGTGGTCGC CGCCGTTCTC TGCATCATGG CGCGCAGGAG CACCAAGACG 
GTGTCCAACT CTGGCAGCTC CATCTACAGT GGACCCTACA CGAACACCGC CTTCTCGCAC 
AGCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286849.1
CDS	89..1477
Translation	MKTGTRMDAFHTALHLITIAALTTHAAQIPTAMKDAQSSLSDAIAAAEAEVASTSKPAVEPSVRIKCLSGSMLITIKDAPPNHETGLFSGMIYPKGLSKNSTCLSEYRDHVGSLRYKLPLRSCNTMPKETDDGGIEFFNTIVLQPHLKLITDLGRGYHVRCAYKSRDAAMKPKKYLRKHAQKPQAFRSDDRREYGRSLDKQQDDDLDEEDVYDANAPTQEEDVTNNEIPMPGCHMKIYNDEHKIADDVKIGDPLTIVISIDKQKVYGLHVTDCIVRDGLGWGEQRLVGEDGCPMDNEIMGQFNYTQDRLAANVTFPAHKFPYTTSVYYQCNVRLCALEDPTCQEAPQCSGKRPKRQAAADSKEEDGLPATIEVFSGLYVNENENANDSDEDAVYKEKTLDDALCVSQRTFAIAIAIAGLILMLAVVAAVLCIMARRSTKTVSNSGSSIYSGPYTNTAFSHSS

Target ORF information:

RefSeq Version	NM_001299920.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster piopio (pio), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299920.1

ORF Insert Sequence:

ATGAAGACAG GCACTCGAAT GGACGCTTTC CACACGGCGC TGCACTTAAT CACAATCGCA 
GCTCTGACGA CGCACGCGGC GCAGATTCCA ACGGCAATGA AAGATGCCCA GAGCTCGCTG 
AGCGATGCGA TTGCGGCGGC GGAGGCGGAG GTGGCCTCCA CCTCTAAGCC GGCGGTGGAG 
CCGAGTGTGC GGATCAAGTG CCTCTCCGGA TCGATGCTGA TCACCATTAA AGACGCTCCT 
CCGAACCACG AAACGGGCCT GTTCAGCGGC ATGATCTACC CGAAAGGCCT GTCCAAGAAC 
TCCACCTGCT TGAGCGAGTA CAGAGATCAT GTGGGCTCCC TGCGATATAA GCTGCCCCTG 
AGATCTTGCA ACACGATGCC GAAGGAAACG GATGACGGTG GCATTGAGTT CTTCAACACC 
ATCGTGCTGC AGCCCCACCT AAAGCTCATC ACGGACCTGG GACGTGGCTA CCACGTCCGC 
TGTGCGTACA AGAGTCGGGA CGCAGCCATG AAGCCCAAAA AGTACCTGCG CAAGCACGCC 
CAGAAGCCAC AGGCCTTCCG CAGCGATGAT CGGCGGGAGT ACGGACGATC GCTGGACAAG 
CAGCAGGACG ACGACCTGGA CGAGGAGGAT GTCTACGATG CGAATGCCCC GACGCAGGAG 
GAGGACGTGA CCAACAACGA GATCCCCATG CCAGGCTGCC ACATGAAGAT CTACAACGAC 
GAGCACAAAA TTGCCGACGA TGTGAAGATC GGTGATCCTC TGACCATTGT CATCAGCATC 
GATAAGCAGA AGGTTTACGG ACTCCATGTC ACGGACTGCA TAGTTCGCGA TGGTCTGGGC 
TGGGGAGAGC AGCGACTGGT GGGCGAAGAT GGCTGCCCCA TGGACAACGA GATCATGGGT 
CAGTTCAACT ACACCCAAGA CCGATTGGCA GCCAACGTGA CCTTTCCGGC GCACAAGTTC 
CCCTACACCA CCTCCGTGTA CTACCAGTGC AACGTGCGAC TGTGCGCCCT GGAGGACCCC 
ACCTGCCAGG AGGCTCCCCA GTGCAGCGGC AAGAGGCCCA AGCGCCAAGC CGCCGCGGAC 
AGCAAGGAGG AGGACGGTCT GCCCGCCACC ATTGAGGTCT TCTCTGGCCT ATATGTGAAT 
GAGAACGAAA ACGCCAACGA CAGCGACGAG GACGCGGTTT ACAAGGAGAA GACTCTGGAC 
GACGCCCTGT GCGTTTCGCA ACGCACCTTC GCCATAGCCA TTGCCATCGC CGGATTGATC 
CTTATGCTAG CCGTGGTCGC CGCCGTTCTC TGCATCATGG CGCGCAGGAG CACCAAGACG 
GTGTCCAACT CTGGCAGCTC CATCTACAGT GGACCCTACA CGAACACCGC CTTCTCGCAC 
AGCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pio ( NM_001169824.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163295.1 Drosophila melanogaster piopio (pio), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa11200
Clone ID Related Accession (Same CDS sequence)	NM_166677.3 , NM_001299920.1 , NM_001169824.2
Accession Version	NM_001169824.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	piopio, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001169824.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGACAG GCACTCGAAT GGACGCTTTC CACACGGCGC TGCACTTAAT CACAATCGCA 
GCTCTGACGA CGCACGCGGC GCAGATTCCA ACGGCAATGA AAGATGCCCA GAGCTCGCTG 
AGCGATGCGA TTGCGGCGGC GGAGGCGGAG GTGGCCTCCA CCTCTAAGCC GGCGGTGGAG 
CCGAGTGTGC GGATCAAGTG CCTCTCCGGA TCGATGCTGA TCACCATTAA AGACGCTCCT 
CCGAACCACG AAACGGGCCT GTTCAGCGGC ATGATCTACC CGAAAGGCCT GTCCAAGAAC 
TCCACCTGCT TGAGCGAGTA CAGAGATCAT GTGGGCTCCC TGCGATATAA GCTGCCCCTG 
AGATCTTGCA ACACGATGCC GAAGGAAACG GATGACGGTG GCATTGAGTT CTTCAACACC 
ATCGTGCTGC AGCCCCACCT AAAGCTCATC ACGGACCTGG GACGTGGCTA CCACGTCCGC 
TGTGCGTACA AGAGTCGGGA CGCAGCCATG AAGCCCAAAA AGTACCTGCG CAAGCACGCC 
CAGAAGCCAC AGGCCTTCCG CAGCGATGAT CGGCGGGAGT ACGGACGATC GCTGGACAAG 
CAGCAGGACG ACGACCTGGA CGAGGAGGAT GTCTACGATG CGAATGCCCC GACGCAGGAG 
GAGGACGTGA CCAACAACGA GATCCCCATG CCAGGCTGCC ACATGAAGAT CTACAACGAC 
GAGCACAAAA TTGCCGACGA TGTGAAGATC GGTGATCCTC TGACCATTGT CATCAGCATC 
GATAAGCAGA AGGTTTACGG ACTCCATGTC ACGGACTGCA TAGTTCGCGA TGGTCTGGGC 
TGGGGAGAGC AGCGACTGGT GGGCGAAGAT GGCTGCCCCA TGGACAACGA GATCATGGGT 
CAGTTCAACT ACACCCAAGA CCGATTGGCA GCCAACGTGA CCTTTCCGGC GCACAAGTTC 
CCCTACACCA CCTCCGTGTA CTACCAGTGC AACGTGCGAC TGTGCGCCCT GGAGGACCCC 
ACCTGCCAGG AGGCTCCCCA GTGCAGCGGC AAGAGGCCCA AGCGCCAAGC CGCCGCGGAC 
AGCAAGGAGG AGGACGGTCT GCCCGCCACC ATTGAGGTCT TCTCTGGCCT ATATGTGAAT 
GAGAACGAAA ACGCCAACGA CAGCGACGAG GACGCGGTTT ACAAGGAGAA GACTCTGGAC 
GACGCCCTGT GCGTTTCGCA ACGCACCTTC GCCATAGCCA TTGCCATCGC CGGATTGATC 
CTTATGCTAG CCGTGGTCGC CGCCGTTCTC TGCATCATGG CGCGCAGGAG CACCAAGACG 
GTGTCCAACT CTGGCAGCTC CATCTACAGT GGACCCTACA CGAACACCGC CTTCTCGCAC 
AGCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163295.1
CDS	843..2231
Translation	MKTGTRMDAFHTALHLITIAALTTHAAQIPTAMKDAQSSLSDAIAAAEAEVASTSKPAVEPSVRIKCLSGSMLITIKDAPPNHETGLFSGMIYPKGLSKNSTCLSEYRDHVGSLRYKLPLRSCNTMPKETDDGGIEFFNTIVLQPHLKLITDLGRGYHVRCAYKSRDAAMKPKKYLRKHAQKPQAFRSDDRREYGRSLDKQQDDDLDEEDVYDANAPTQEEDVTNNEIPMPGCHMKIYNDEHKIADDVKIGDPLTIVISIDKQKVYGLHVTDCIVRDGLGWGEQRLVGEDGCPMDNEIMGQFNYTQDRLAANVTFPAHKFPYTTSVYYQCNVRLCALEDPTCQEAPQCSGKRPKRQAAADSKEEDGLPATIEVFSGLYVNENENANDSDEDAVYKEKTLDDALCVSQRTFAIAIAIAGLILMLAVVAAVLCIMARRSTKTVSNSGSSIYSGPYTNTAFSHSS

Target ORF information:

RefSeq Version	NM_001169824.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster piopio (pio), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169824.2

ORF Insert Sequence:

ATGAAGACAG GCACTCGAAT GGACGCTTTC CACACGGCGC TGCACTTAAT CACAATCGCA 
GCTCTGACGA CGCACGCGGC GCAGATTCCA ACGGCAATGA AAGATGCCCA GAGCTCGCTG 
AGCGATGCGA TTGCGGCGGC GGAGGCGGAG GTGGCCTCCA CCTCTAAGCC GGCGGTGGAG 
CCGAGTGTGC GGATCAAGTG CCTCTCCGGA TCGATGCTGA TCACCATTAA AGACGCTCCT 
CCGAACCACG AAACGGGCCT GTTCAGCGGC ATGATCTACC CGAAAGGCCT GTCCAAGAAC 
TCCACCTGCT TGAGCGAGTA CAGAGATCAT GTGGGCTCCC TGCGATATAA GCTGCCCCTG 
AGATCTTGCA ACACGATGCC GAAGGAAACG GATGACGGTG GCATTGAGTT CTTCAACACC 
ATCGTGCTGC AGCCCCACCT AAAGCTCATC ACGGACCTGG GACGTGGCTA CCACGTCCGC 
TGTGCGTACA AGAGTCGGGA CGCAGCCATG AAGCCCAAAA AGTACCTGCG CAAGCACGCC 
CAGAAGCCAC AGGCCTTCCG CAGCGATGAT CGGCGGGAGT ACGGACGATC GCTGGACAAG 
CAGCAGGACG ACGACCTGGA CGAGGAGGAT GTCTACGATG CGAATGCCCC GACGCAGGAG 
GAGGACGTGA CCAACAACGA GATCCCCATG CCAGGCTGCC ACATGAAGAT CTACAACGAC 
GAGCACAAAA TTGCCGACGA TGTGAAGATC GGTGATCCTC TGACCATTGT CATCAGCATC 
GATAAGCAGA AGGTTTACGG ACTCCATGTC ACGGACTGCA TAGTTCGCGA TGGTCTGGGC 
TGGGGAGAGC AGCGACTGGT GGGCGAAGAT GGCTGCCCCA TGGACAACGA GATCATGGGT 
CAGTTCAACT ACACCCAAGA CCGATTGGCA GCCAACGTGA CCTTTCCGGC GCACAAGTTC 
CCCTACACCA CCTCCGTGTA CTACCAGTGC AACGTGCGAC TGTGCGCCCT GGAGGACCCC 
ACCTGCCAGG AGGCTCCCCA GTGCAGCGGC AAGAGGCCCA AGCGCCAAGC CGCCGCGGAC 
AGCAAGGAGG AGGACGGTCT GCCCGCCACC ATTGAGGTCT TCTCTGGCCT ATATGTGAAT 
GAGAACGAAA ACGCCAACGA CAGCGACGAG GACGCGGTTT ACAAGGAGAA GACTCTGGAC 
GACGCCCTGT GCGTTTCGCA ACGCACCTTC GCCATAGCCA TTGCCATCGC CGGATTGATC 
CTTATGCTAG CCGTGGTCGC CGCCGTTCTC TGCATCATGG CGCGCAGGAG CACCAAGACG 
GTGTCCAACT CTGGCAGCTC CATCTACAGT GGACCCTACA CGAACACCGC CTTCTCGCAC 
AGCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pio ( NM_138094.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611938.2 Drosophila melanogaster piopio (pio), transcript variant C, mRNA.

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CloneID	OFa11201
Clone ID Related Accession (Same CDS sequence)	NM_138094.4
Accession Version	NM_138094.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	piopio, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_138094.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGACAG GCACTCGAAT GGACGCTTTC CACACGGCGC TGCACTTAAT CACAATCGCA 
GCTCTGACGA CGCACGCGGC GCAGATTCCA ACGGCAATGA AAGATGCCCA GAGCTCGCTG 
AGCGATGCGA TTGCGGCGGC GGAGGCGGAG GTGGCCTCCA CCTCTAAGCC GGCGGTGGAG 
CCGAGTGTGC GGATCAAGTG CCTCTCCGGA TCGATGCTGA TCACCATTAA AGACGCTCCT 
CCGAACCACG AAACGGGCCT GTTCAGCGGC ATGATCTACC CGAAAGGCCT GTCCAAGAAC 
TCCACCTGCT TGAGCGAGTA CAGAGATCAT GTGGGCTCCC TGCGATATAA GCTGCCCCTG 
AGATCTTGCA ACACGATGCC GAAGGAAACG GTGACGCCAC GTACCGGGCA ACTCGTTCTG 
CCATTTTGGC ATGCAAATAA AAAATTAAAC ATTAAGTTTA AGCACTTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611938.2
CDS	367..837
Translation	MKTGTRMDAFHTALHLITIAALTTHAAQIPTAMKDAQSSLSDAIAAAEAEVASTSKPAVEPSVRIKCLSGSMLITIKDAPPNHETGLFSGMIYPKGLSKNSTCLSEYRDHVGSLRYKLPLRSCNTMPKETVTPRTGQLVLPFWHANKKLNIKFKHL

Target ORF information:

RefSeq Version	NM_138094.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster piopio (pio), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138094.4

ORF Insert Sequence:

ATGAAGACAG GCACTCGAAT GGACGCTTTC CACACGGCGC TGCACTTAAT CACAATCGCA 
GCTCTGACGA CGCACGCGGC GCAGATTCCA ACGGCAATGA AAGATGCCCA GAGCTCGCTG 
AGCGATGCGA TTGCGGCGGC GGAGGCGGAG GTGGCCTCCA CCTCTAAGCC GGCGGTGGAG 
CCGAGTGTGC GGATCAAGTG CCTCTCCGGA TCGATGCTGA TCACCATTAA AGACGCTCCT 
CCGAACCACG AAACGGGCCT GTTCAGCGGC ATGATCTACC CGAAAGGCCT GTCCAAGAAC 
TCCACCTGCT TGAGCGAGTA CAGAGATCAT GTGGGCTCCC TGCGATATAA GCTGCCCCTG 
AGATCTTGCA ACACGATGCC GAAGGAAACG GTGACGCCAC GTACCGGGCA ACTCGTTCTG 
CCATTTTGGC ATGCAAATAA AAAATTAAAC ATTAAGTTTA AGCACTTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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pio cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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