Start1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Start1
Entrez Gene ID	37927
Full Name	CG3522 gene product from transcript CG3522-RB
Synonyms	CG3522,Dmel\CG3522,StART1,Start,start1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 60D5-60D5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001299919.1	NP_001286848.1	Start1, isoform D
NM_206217.3	NP_995939.1	Start1, isoform B
NM_138092.3	NP_611936.2	Start1, isoform A
NM_001259566.2	NP_001246495.1	Start1, isoform C

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Stard3	NP_001014251.1
Gallus gallus (chicken)	STARD3	XP_001232168.1
Danio rerio (zebrafish)	stard3	NP_571737.1
	STARD3	XP_001089498.1
Canis lupus familiaris (dog)	STARD3	XP_005624614.1
Xenopus tropicalis (tropical clawed frog)	stard3	NP_001006799.1
Homo sapiens (human)	STARD3	NP_006795.3
Pan troglodytes (chimpanzee)	STARD3	XP_001172256.1
Bos taurus (cattle)	STARD3	NP_001180072.1
Mus musculus (house mouse)	Stard3	NP_067522.1
Drosophila melanogaster (fruit fly)	Start1	NP_611936.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007057	XP_308707.4
Caenorhabditis elegans (roundworm)	tag-340	NP_498027.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

plays a key role in the regulation of ecdysteroid synthesis
Title: The Drosophila gene Start1: a putative cholesterol transporter and key regulator of ecdysteroid synthesis.

The following Start1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Start1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa11197	NM_206217.3 Latest version!	Drosophila melanogaster Start1, transcript variant B (Start1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa11198	NM_138092.3 Latest version!	Drosophila melanogaster Start1, transcript variant A (Start1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa11198	NM_001259566.2 Latest version!	Drosophila melanogaster Start1, transcript variant C (Start1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa21953	NM_001299919.1 Latest version!	Drosophila melanogaster Start1, transcript variant D (Start1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Start1 ( NM_206217.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995939.1 Drosophila melanogaster Start1, transcript variant B (Start1), mRNA.

Price & Availability↑

CloneID	OFa11197
Clone ID Related Accession (Same CDS sequence)	NM_206217.3
Accession Version	NM_206217.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Start1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_206217.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAATA TGGATCCAAG TGATGTCCGC AGTACCGCCC AGCTCATCCT GGCCAACGCT 
CGGCAGGGAA ATAGCGCTTA CAACATGCAG TATGATATGT CACGGGCACA TTCCATAAAC 
CTGATTACGG AGGACTTCTT GGCCGGTTAT ATGCAGGATG GCAGGATGTC CGTGGTTCGA 
AGGTTTTTCT GCCTCTTCGT CACATTCGAC TTGGTCTTCG TTTCGCTGCT GTGGCTCATT 
TGCATTGTGA TCAATGGAGA CAATATATTC ACTGCCTTCC ACAAACAAAT CGTGGAGTAC 
ACCATCTACA AATCGCTCTT TGATGTTGTG GCAGTCGCTA TCTGCCGATT TTTGGTGCTC 
ATATTTTTCT ACGCCATATT GTACATCAAT CACTGGTCCA TCATAGCGCT CTCTACAAGT 
GGGTCTTGCT TGTTCCTCAT CTCGAAGGTG TTTGTGTTCG ATTGGCTGGA TTCAAAGCAG 
CAGGTATTTG AGGTAATCCT CATAATAACC TCGTTCATAC TGGCTTGGGG AGAAGCCTGG 
TTCCTGGACT GTAGGGTGAT TCCTCAAGAG CGACATGCCC AACACTATTT CCGGACTATG 
ACTTCAAATG ATCGCACACC CATGGAACAG CCTGCCATTT TGATTGAGCA AGAACGGCCT 
CCGCAAAGTG TAACCGATTT TTATTCACTC ATGGACACGG CTCGTCATTC CGACGAGGAG 
GATGAGTTGG ATGATGAGTA CACACAAATG GGATTGGATT GCCTTCGAAA GGCCTACGAG 
ATCATCGAGT CAAGTGACTG GAAGGTGGAA AAAGTTAACC AGAAAGGCGA CACCATACAC 
AGCACTCAGC GCGACAAGAT TGGAAAGATC TACAAGTTGA CGGCCCGCAT CAAGTATCCT 
GCAAAGGCTC TGATGGAAGA TCTGTTCTAT CGCATTGAAG ACTGTCCCAA GTGGAATCCT 
GCTCTTTTGG AGTCCAAGAT AGTACGCAAA ATCAACTCCT ACACCGATAT TACCTATCAG 
GTATCCGTGG GCGGAGGAGG TGGCATGGTG AAGAGCCGCG ACTTCGTGAA CTTGCGGTCT 
TGTAGGCTCT TTTACAATGG TCAAATCTGC GATGACGATG AGACGGCTCA GCTCAGCAGC 
GATGATGGGA ACAGCAGTCT AAATCGGTCT TGCGAGGGTA GTGTTAGTAC CATTTCCGAT 
GGTGACTCAA ACACCCCACT GCTGCCCAGT AGCGTGTCTA GTTGCAAGGC AACGTTTCCC 
ACTTCATCCA AGGGAGCCGC TATGCCTTTT GACACCCTGG GCAACAGCTT GGGCGCCAAG 
AGCCTAGGTC CCATCGTGAA CTTTGACGAG GAGCCACCGC CATTGGATCA GGACGAGTTC 
GAGGATGCTA AGGACAAGGT CGACGGCGAA GCGAATAACA TGACGAAACC AAATGTACCC 
AGCGTGGGAA AAACCAAGGA CAGGGTTTGG GTCACTTCGG CGGTTAGTGT GCAATATGCT 
GCCGTTCCAC CTTCACCCAA ATACACAAGT CGGTTGACTC TTCAATTTTC AGAGGGCAGA 
ACATTGTCAG TGGGTTTGCG TTTCGTGAAA TCGTTGGAAA ATCGGATAGC TGCATTGTGG 
AGTGGGTGCT TTGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995939.1
CDS	374..2011
Translation	MSNMDPSDVRSTAQLILANARQGNSAYNMQYDMSRAHSINLITEDFLAGYMQDGRMSVVRRFFCLFVTFDLVFVSLLWLICIVINGDNIFTAFHKQIVEYTIYKSLFDVVAVAICRFLVLIFFYAILYINHWSIIALSTSGSCLFLISKVFVFDWLDSKQQVFEVILIITSFILAWGEAWFLDCRVIPQERHAQHYFRTMTSNDRTPMEQPAILIEQERPPQSVTDFYSLMDTARHSDEEDELDDEYTQMGLDCLRKAYEIIESSDWKVEKVNQKGDTIHSTQRDKIGKIYKLTARIKYPAKALMEDLFYRIEDCPKWNPALLESKIVRKINSYTDITYQVSVGGGGGMVKSRDFVNLRSCRLFYNGQICDDDETAQLSSDDGNSSLNRSCEGSVSTISDGDSNTPLLPSSVSSCKATFPTSSKGAAMPFDTLGNSLGAKSLGPIVNFDEEPPPLDQDEFEDAKDKVDGEANNMTKPNVPSVGKTKDRVWVTSAVSVQYAAVPPSPKYTSRLTLQFSEGRTLSVGLRFVKSLENRIAALWSGCFA

Target ORF information:

RefSeq Version	NM_206217.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Start1, transcript variant B (Start1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206217.3

ORF Insert Sequence:

ATGTCCAATA TGGATCCAAG TGATGTCCGC AGTACCGCCC AGCTCATCCT GGCCAACGCT 
CGGCAGGGAA ATAGCGCTTA CAACATGCAG TATGATATGT CACGGGCACA TTCCATAAAC 
CTGATTACGG AGGACTTCTT GGCCGGTTAT ATGCAGGATG GCAGGATGTC CGTGGTTCGA 
AGGTTTTTCT GCCTCTTCGT CACATTCGAC TTGGTCTTCG TTTCGCTGCT GTGGCTCATT 
TGCATTGTGA TCAATGGAGA CAATATATTC ACTGCCTTCC ACAAACAAAT CGTGGAGTAC 
ACCATCTACA AATCGCTCTT TGATGTTGTG GCAGTCGCTA TCTGCCGATT TTTGGTGCTC 
ATATTTTTCT ACGCCATATT GTACATCAAT CACTGGTCCA TCATAGCGCT CTCTACAAGT 
GGGTCTTGCT TGTTCCTCAT CTCGAAGGTG TTTGTGTTCG ATTGGCTGGA TTCAAAGCAG 
CAGGTATTTG AGGTAATCCT CATAATAACC TCGTTCATAC TGGCTTGGGG AGAAGCCTGG 
TTCCTGGACT GTAGGGTGAT TCCTCAAGAG CGACATGCCC AACACTATTT CCGGACTATG 
ACTTCAAATG ATCGCACACC CATGGAACAG CCTGCCATTT TGATTGAGCA AGAACGGCCT 
CCGCAAAGTG TAACCGATTT TTATTCACTC ATGGACACGG CTCGTCATTC CGACGAGGAG 
GATGAGTTGG ATGATGAGTA CACACAAATG GGATTGGATT GCCTTCGAAA GGCCTACGAG 
ATCATCGAGT CAAGTGACTG GAAGGTGGAA AAAGTTAACC AGAAAGGCGA CACCATACAC 
AGCACTCAGC GCGACAAGAT TGGAAAGATC TACAAGTTGA CGGCCCGCAT CAAGTATCCT 
GCAAAGGCTC TGATGGAAGA TCTGTTCTAT CGCATTGAAG ACTGTCCCAA GTGGAATCCT 
GCTCTTTTGG AGTCCAAGAT AGTACGCAAA ATCAACTCCT ACACCGATAT TACCTATCAG 
GTATCCGTGG GCGGAGGAGG TGGCATGGTG AAGAGCCGCG ACTTCGTGAA CTTGCGGTCT 
TGTAGGCTCT TTTACAATGG TCAAATCTGC GATGACGATG AGACGGCTCA GCTCAGCAGC 
GATGATGGGA ACAGCAGTCT AAATCGGTCT TGCGAGGGTA GTGTTAGTAC CATTTCCGAT 
GGTGACTCAA ACACCCCACT GCTGCCCAGT AGCGTGTCTA GTTGCAAGGC AACGTTTCCC 
ACTTCATCCA AGGGAGCCGC TATGCCTTTT GACACCCTGG GCAACAGCTT GGGCGCCAAG 
AGCCTAGGTC CCATCGTGAA CTTTGACGAG GAGCCACCGC CATTGGATCA GGACGAGTTC 
GAGGATGCTA AGGACAAGGT CGACGGCGAA GCGAATAACA TGACGAAACC AAATGTACCC 
AGCGTGGGAA AAACCAAGGA CAGGGTTTGG GTCACTTCGG CGGTTAGTGT GCAATATGCT 
GCCGTTCCAC CTTCACCCAA ATACACAAGT CGGTTGACTC TTCAATTTTC AGAGGGCAGA 
ACATTGTCAG TGGGTTTGCG TTTCGTGAAA TCGTTGGAAA ATCGGATAGC TGCATTGTGG 
AGTGGGTGCT TTGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Start1 ( NM_138092.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611936.2 Drosophila melanogaster Start1, transcript variant A (Start1), mRNA.

Price & Availability↑

CloneID	OFa11198
Clone ID Related Accession (Same CDS sequence)	NM_138092.3 , NM_001259566.2
Accession Version	NM_138092.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Start1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_138092.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAATA TGGATCCAAG TGATGTCCGC AGTACCGCCC AGCTCATCCT GGCCAACGCT 
CGGCAGGGAA ATAGCGCTTA CAACATGCAG TATGATATGT CACGGGCACA TTCCATAAAC 
CTGATTACGG AGGACTTCTT GGCCGGTTAT ATGCAGGATG GCAGGATGTC CGTGGTTCGA 
AGGTTTTTCT GCCTCTTCGT CACATTCGAC TTGGTCTTCG TTTCGCTGCT GTGGCTCATT 
TGCATTGTGA TCAATGGAGA CAATATATTC ACTGCCTTCC ACAAACAAAT CGTGGAGTAC 
ACCATCTACA AATCGCTCTT TGATGTTGTG GCAGTCGCTA TCTGCCGATT TTTGGTGCTC 
ATATTTTTCT ACGCCATATT GTACATCAAT CACTGGTCCA TCATAGCGCT CTCTACAAGT 
GGGTCTTGCT TGTTCCTCAT CTCGAAGGTG TTTGTGTTCG ATTGGCTGGA TTCAAAGCAG 
CAGGTATTTG AGGTAATCCT CATAATAACC TCGTTCATAC TGGCTTGGGG AGAAGCCTGG 
TTCCTGGACT GTAGGGTGAT TCCTCAAGAG CGACATGCCC AACACTATTT CCGGACTATG 
ACTTCAAATG ATCGCACACC CATGGAACAG CCTGCCATTT TGATTGAGCA AGAACGGCCT 
CCGCAAAGTG TAACCGATTT TTATTCACTC ATGGACACGG CTCGTCATTC CGACGAGGAG 
GATGAGTTGG ATGATGAGTA CACACAAATG GGATTGGATT GCCTTCGAAA GGCCTACGAG 
ATCATCGAGT CAAGTGACTG GAAGGTGGAA AAAGTTAACC AGAAAGGCGA CACCATACAC 
AGCACTCAGC GCGACAAGAT TGGAAAGATC TACAAGTTGA CGGCCCGCAT CAAGTATCCT 
GCAAAGGCTC TGATGGAAGA TCTGTTCTAT CGCATTGAAG ACTGTCCCAA GTGGAATCCT 
GCTCTTTTGG AGTCCAAGAT AGTACGCAAA ATCAACTCCT ACACCGATAT TACCTATCAG 
GTATCCGTGG GCGGAGGAGG TGGCATGGTG AAGAGCCGCG ACTTCGTGAA CTTGCGGTCT 
TGTAGGCTCT TTTACAATGG TCAAATCTGC GATGACGATG AGACGGCTCA GCTCAGCAGC 
GATGATGGGA ACAGCAGTCT AAATCGGTCT TGCGAGGGTA GTGTTAGTAC CATTTCCGAT 
GGTGACTCAA ACACCCCACT GCTGCCCAGT AGCGTGTCTA GTTGCAAGGC AACGTTTCCC 
ACTTCATCCA AGGGAGCCGC TATGCCTTTT GACACCCTGG GCAACAGCTT GGGCGCCAAG 
AGCCTAGGTC CCATCGTGAA CTTTGACGAG GAGCCACCGC CATTGGATCA GGACGAGTTC 
GAGGATGCTA AGGACAAGGT CGACGGCGAA GCGAATAACA TGACGAAACC AAATGTACCC 
AGCGTGGGAA AAACCAAGGA CAGGGTTTGG GTCACTTCGG CGGTTAGTGT GCAATATGCT 
GCCGTTCCAC CTTCACCCAA ATACACAAGA GGGCAGAACA TTGTCAGTGG GTTTGCGTTT 
CGTGAAATCG TTGGAAAATC GGATAGCTGC ATTGTGGAGT GGGTGCTTTG CCTAGACCTA 
AAAGGCTATA TTCCTCGCTA TGTCCTGGAT GCAGCCCTCA CCTCGTCCAT GACCGACTAC 
ATTAGCAATC TGCGCAAGCA TGTCAACGAA CTGAGGCAGA AGGGGCGTGG TCGAGCCCCC 
AGGACCCACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611936.2
CDS	207..1958
Translation	MSNMDPSDVRSTAQLILANARQGNSAYNMQYDMSRAHSINLITEDFLAGYMQDGRMSVVRRFFCLFVTFDLVFVSLLWLICIVINGDNIFTAFHKQIVEYTIYKSLFDVVAVAICRFLVLIFFYAILYINHWSIIALSTSGSCLFLISKVFVFDWLDSKQQVFEVILIITSFILAWGEAWFLDCRVIPQERHAQHYFRTMTSNDRTPMEQPAILIEQERPPQSVTDFYSLMDTARHSDEEDELDDEYTQMGLDCLRKAYEIIESSDWKVEKVNQKGDTIHSTQRDKIGKIYKLTARIKYPAKALMEDLFYRIEDCPKWNPALLESKIVRKINSYTDITYQVSVGGGGGMVKSRDFVNLRSCRLFYNGQICDDDETAQLSSDDGNSSLNRSCEGSVSTISDGDSNTPLLPSSVSSCKATFPTSSKGAAMPFDTLGNSLGAKSLGPIVNFDEEPPPLDQDEFEDAKDKVDGEANNMTKPNVPSVGKTKDRVWVTSAVSVQYAAVPPSPKYTRGQNIVSGFAFREIVGKSDSCIVEWVLCLDLKGYIPRYVLDAALTSSMTDYISNLRKHVNELRQKGRGRAPRTH

Target ORF information:

RefSeq Version	NM_138092.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Start1, transcript variant A (Start1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138092.3

ORF Insert Sequence:

ATGTCCAATA TGGATCCAAG TGATGTCCGC AGTACCGCCC AGCTCATCCT GGCCAACGCT 
CGGCAGGGAA ATAGCGCTTA CAACATGCAG TATGATATGT CACGGGCACA TTCCATAAAC 
CTGATTACGG AGGACTTCTT GGCCGGTTAT ATGCAGGATG GCAGGATGTC CGTGGTTCGA 
AGGTTTTTCT GCCTCTTCGT CACATTCGAC TTGGTCTTCG TTTCGCTGCT GTGGCTCATT 
TGCATTGTGA TCAATGGAGA CAATATATTC ACTGCCTTCC ACAAACAAAT CGTGGAGTAC 
ACCATCTACA AATCGCTCTT TGATGTTGTG GCAGTCGCTA TCTGCCGATT TTTGGTGCTC 
ATATTTTTCT ACGCCATATT GTACATCAAT CACTGGTCCA TCATAGCGCT CTCTACAAGT 
GGGTCTTGCT TGTTCCTCAT CTCGAAGGTG TTTGTGTTCG ATTGGCTGGA TTCAAAGCAG 
CAGGTATTTG AGGTAATCCT CATAATAACC TCGTTCATAC TGGCTTGGGG AGAAGCCTGG 
TTCCTGGACT GTAGGGTGAT TCCTCAAGAG CGACATGCCC AACACTATTT CCGGACTATG 
ACTTCAAATG ATCGCACACC CATGGAACAG CCTGCCATTT TGATTGAGCA AGAACGGCCT 
CCGCAAAGTG TAACCGATTT TTATTCACTC ATGGACACGG CTCGTCATTC CGACGAGGAG 
GATGAGTTGG ATGATGAGTA CACACAAATG GGATTGGATT GCCTTCGAAA GGCCTACGAG 
ATCATCGAGT CAAGTGACTG GAAGGTGGAA AAAGTTAACC AGAAAGGCGA CACCATACAC 
AGCACTCAGC GCGACAAGAT TGGAAAGATC TACAAGTTGA CGGCCCGCAT CAAGTATCCT 
GCAAAGGCTC TGATGGAAGA TCTGTTCTAT CGCATTGAAG ACTGTCCCAA GTGGAATCCT 
GCTCTTTTGG AGTCCAAGAT AGTACGCAAA ATCAACTCCT ACACCGATAT TACCTATCAG 
GTATCCGTGG GCGGAGGAGG TGGCATGGTG AAGAGCCGCG ACTTCGTGAA CTTGCGGTCT 
TGTAGGCTCT TTTACAATGG TCAAATCTGC GATGACGATG AGACGGCTCA GCTCAGCAGC 
GATGATGGGA ACAGCAGTCT AAATCGGTCT TGCGAGGGTA GTGTTAGTAC CATTTCCGAT 
GGTGACTCAA ACACCCCACT GCTGCCCAGT AGCGTGTCTA GTTGCAAGGC AACGTTTCCC 
ACTTCATCCA AGGGAGCCGC TATGCCTTTT GACACCCTGG GCAACAGCTT GGGCGCCAAG 
AGCCTAGGTC CCATCGTGAA CTTTGACGAG GAGCCACCGC CATTGGATCA GGACGAGTTC 
GAGGATGCTA AGGACAAGGT CGACGGCGAA GCGAATAACA TGACGAAACC AAATGTACCC 
AGCGTGGGAA AAACCAAGGA CAGGGTTTGG GTCACTTCGG CGGTTAGTGT GCAATATGCT 
GCCGTTCCAC CTTCACCCAA ATACACAAGA GGGCAGAACA TTGTCAGTGG GTTTGCGTTT 
CGTGAAATCG TTGGAAAATC GGATAGCTGC ATTGTGGAGT GGGTGCTTTG CCTAGACCTA 
AAAGGCTATA TTCCTCGCTA TGTCCTGGAT GCAGCCCTCA CCTCGTCCAT GACCGACTAC 
ATTAGCAATC TGCGCAAGCA TGTCAACGAA CTGAGGCAGA AGGGGCGTGG TCGAGCCCCC 
AGGACCCACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Start1 ( NM_001259566.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246495.1 Drosophila melanogaster Start1, transcript variant C (Start1), mRNA.

Price & Availability↑

CloneID	OFa11198
Clone ID Related Accession (Same CDS sequence)	NM_138092.3 , NM_001259566.2
Accession Version	NM_001259566.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Start1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001259566.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAATA TGGATCCAAG TGATGTCCGC AGTACCGCCC AGCTCATCCT GGCCAACGCT 
CGGCAGGGAA ATAGCGCTTA CAACATGCAG TATGATATGT CACGGGCACA TTCCATAAAC 
CTGATTACGG AGGACTTCTT GGCCGGTTAT ATGCAGGATG GCAGGATGTC CGTGGTTCGA 
AGGTTTTTCT GCCTCTTCGT CACATTCGAC TTGGTCTTCG TTTCGCTGCT GTGGCTCATT 
TGCATTGTGA TCAATGGAGA CAATATATTC ACTGCCTTCC ACAAACAAAT CGTGGAGTAC 
ACCATCTACA AATCGCTCTT TGATGTTGTG GCAGTCGCTA TCTGCCGATT TTTGGTGCTC 
ATATTTTTCT ACGCCATATT GTACATCAAT CACTGGTCCA TCATAGCGCT CTCTACAAGT 
GGGTCTTGCT TGTTCCTCAT CTCGAAGGTG TTTGTGTTCG ATTGGCTGGA TTCAAAGCAG 
CAGGTATTTG AGGTAATCCT CATAATAACC TCGTTCATAC TGGCTTGGGG AGAAGCCTGG 
TTCCTGGACT GTAGGGTGAT TCCTCAAGAG CGACATGCCC AACACTATTT CCGGACTATG 
ACTTCAAATG ATCGCACACC CATGGAACAG CCTGCCATTT TGATTGAGCA AGAACGGCCT 
CCGCAAAGTG TAACCGATTT TTATTCACTC ATGGACACGG CTCGTCATTC CGACGAGGAG 
GATGAGTTGG ATGATGAGTA CACACAAATG GGATTGGATT GCCTTCGAAA GGCCTACGAG 
ATCATCGAGT CAAGTGACTG GAAGGTGGAA AAAGTTAACC AGAAAGGCGA CACCATACAC 
AGCACTCAGC GCGACAAGAT TGGAAAGATC TACAAGTTGA CGGCCCGCAT CAAGTATCCT 
GCAAAGGCTC TGATGGAAGA TCTGTTCTAT CGCATTGAAG ACTGTCCCAA GTGGAATCCT 
GCTCTTTTGG AGTCCAAGAT AGTACGCAAA ATCAACTCCT ACACCGATAT TACCTATCAG 
GTATCCGTGG GCGGAGGAGG TGGCATGGTG AAGAGCCGCG ACTTCGTGAA CTTGCGGTCT 
TGTAGGCTCT TTTACAATGG TCAAATCTGC GATGACGATG AGACGGCTCA GCTCAGCAGC 
GATGATGGGA ACAGCAGTCT AAATCGGTCT TGCGAGGGTA GTGTTAGTAC CATTTCCGAT 
GGTGACTCAA ACACCCCACT GCTGCCCAGT AGCGTGTCTA GTTGCAAGGC AACGTTTCCC 
ACTTCATCCA AGGGAGCCGC TATGCCTTTT GACACCCTGG GCAACAGCTT GGGCGCCAAG 
AGCCTAGGTC CCATCGTGAA CTTTGACGAG GAGCCACCGC CATTGGATCA GGACGAGTTC 
GAGGATGCTA AGGACAAGGT CGACGGCGAA GCGAATAACA TGACGAAACC AAATGTACCC 
AGCGTGGGAA AAACCAAGGA CAGGGTTTGG GTCACTTCGG CGGTTAGTGT GCAATATGCT 
GCCGTTCCAC CTTCACCCAA ATACACAAGA GGGCAGAACA TTGTCAGTGG GTTTGCGTTT 
CGTGAAATCG TTGGAAAATC GGATAGCTGC ATTGTGGAGT GGGTGCTTTG CCTAGACCTA 
AAAGGCTATA TTCCTCGCTA TGTCCTGGAT GCAGCCCTCA CCTCGTCCAT GACCGACTAC 
ATTAGCAATC TGCGCAAGCA TGTCAACGAA CTGAGGCAGA AGGGGCGTGG TCGAGCCCCC 
AGGACCCACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246495.1
CDS	374..2125
Translation	MSNMDPSDVRSTAQLILANARQGNSAYNMQYDMSRAHSINLITEDFLAGYMQDGRMSVVRRFFCLFVTFDLVFVSLLWLICIVINGDNIFTAFHKQIVEYTIYKSLFDVVAVAICRFLVLIFFYAILYINHWSIIALSTSGSCLFLISKVFVFDWLDSKQQVFEVILIITSFILAWGEAWFLDCRVIPQERHAQHYFRTMTSNDRTPMEQPAILIEQERPPQSVTDFYSLMDTARHSDEEDELDDEYTQMGLDCLRKAYEIIESSDWKVEKVNQKGDTIHSTQRDKIGKIYKLTARIKYPAKALMEDLFYRIEDCPKWNPALLESKIVRKINSYTDITYQVSVGGGGGMVKSRDFVNLRSCRLFYNGQICDDDETAQLSSDDGNSSLNRSCEGSVSTISDGDSNTPLLPSSVSSCKATFPTSSKGAAMPFDTLGNSLGAKSLGPIVNFDEEPPPLDQDEFEDAKDKVDGEANNMTKPNVPSVGKTKDRVWVTSAVSVQYAAVPPSPKYTRGQNIVSGFAFREIVGKSDSCIVEWVLCLDLKGYIPRYVLDAALTSSMTDYISNLRKHVNELRQKGRGRAPRTH

Target ORF information:

RefSeq Version	NM_001259566.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Start1, transcript variant C (Start1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259566.2

ORF Insert Sequence:

ATGTCCAATA TGGATCCAAG TGATGTCCGC AGTACCGCCC AGCTCATCCT GGCCAACGCT 
CGGCAGGGAA ATAGCGCTTA CAACATGCAG TATGATATGT CACGGGCACA TTCCATAAAC 
CTGATTACGG AGGACTTCTT GGCCGGTTAT ATGCAGGATG GCAGGATGTC CGTGGTTCGA 
AGGTTTTTCT GCCTCTTCGT CACATTCGAC TTGGTCTTCG TTTCGCTGCT GTGGCTCATT 
TGCATTGTGA TCAATGGAGA CAATATATTC ACTGCCTTCC ACAAACAAAT CGTGGAGTAC 
ACCATCTACA AATCGCTCTT TGATGTTGTG GCAGTCGCTA TCTGCCGATT TTTGGTGCTC 
ATATTTTTCT ACGCCATATT GTACATCAAT CACTGGTCCA TCATAGCGCT CTCTACAAGT 
GGGTCTTGCT TGTTCCTCAT CTCGAAGGTG TTTGTGTTCG ATTGGCTGGA TTCAAAGCAG 
CAGGTATTTG AGGTAATCCT CATAATAACC TCGTTCATAC TGGCTTGGGG AGAAGCCTGG 
TTCCTGGACT GTAGGGTGAT TCCTCAAGAG CGACATGCCC AACACTATTT CCGGACTATG 
ACTTCAAATG ATCGCACACC CATGGAACAG CCTGCCATTT TGATTGAGCA AGAACGGCCT 
CCGCAAAGTG TAACCGATTT TTATTCACTC ATGGACACGG CTCGTCATTC CGACGAGGAG 
GATGAGTTGG ATGATGAGTA CACACAAATG GGATTGGATT GCCTTCGAAA GGCCTACGAG 
ATCATCGAGT CAAGTGACTG GAAGGTGGAA AAAGTTAACC AGAAAGGCGA CACCATACAC 
AGCACTCAGC GCGACAAGAT TGGAAAGATC TACAAGTTGA CGGCCCGCAT CAAGTATCCT 
GCAAAGGCTC TGATGGAAGA TCTGTTCTAT CGCATTGAAG ACTGTCCCAA GTGGAATCCT 
GCTCTTTTGG AGTCCAAGAT AGTACGCAAA ATCAACTCCT ACACCGATAT TACCTATCAG 
GTATCCGTGG GCGGAGGAGG TGGCATGGTG AAGAGCCGCG ACTTCGTGAA CTTGCGGTCT 
TGTAGGCTCT TTTACAATGG TCAAATCTGC GATGACGATG AGACGGCTCA GCTCAGCAGC 
GATGATGGGA ACAGCAGTCT AAATCGGTCT TGCGAGGGTA GTGTTAGTAC CATTTCCGAT 
GGTGACTCAA ACACCCCACT GCTGCCCAGT AGCGTGTCTA GTTGCAAGGC AACGTTTCCC 
ACTTCATCCA AGGGAGCCGC TATGCCTTTT GACACCCTGG GCAACAGCTT GGGCGCCAAG 
AGCCTAGGTC CCATCGTGAA CTTTGACGAG GAGCCACCGC CATTGGATCA GGACGAGTTC 
GAGGATGCTA AGGACAAGGT CGACGGCGAA GCGAATAACA TGACGAAACC AAATGTACCC 
AGCGTGGGAA AAACCAAGGA CAGGGTTTGG GTCACTTCGG CGGTTAGTGT GCAATATGCT 
GCCGTTCCAC CTTCACCCAA ATACACAAGA GGGCAGAACA TTGTCAGTGG GTTTGCGTTT 
CGTGAAATCG TTGGAAAATC GGATAGCTGC ATTGTGGAGT GGGTGCTTTG CCTAGACCTA 
AAAGGCTATA TTCCTCGCTA TGTCCTGGAT GCAGCCCTCA CCTCGTCCAT GACCGACTAC 
ATTAGCAATC TGCGCAAGCA TGTCAACGAA CTGAGGCAGA AGGGGCGTGG TCGAGCCCCC 
AGGACCCACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Start1 ( NM_001299919.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286848.1 Drosophila melanogaster Start1, transcript variant D (Start1), mRNA.

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CloneID	OFa21953
Clone ID Related Accession (Same CDS sequence)	NM_001299919.1
Accession Version	NM_001299919.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Start1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACATCGG ATGATGAGTA CACACAAATG GGATTGGATT GCCTTCGAAA GGCCTACGAG 
ATCATCGAGT CAAGTGACTG GAAGGTGGAA AAAGTTAACC AGAAAGGCGA CACCATACAC 
AGCACTCAGC GCGACAAGAT TGGAAAGATC TACAAGTTGA CGGCCCGCAT CAAGTATCCT 
GCAAAGGCTC TGATGGAAGA TCTGTTCTAT CGCATTGAAG ACTGTCCCAA GTGGAATCCT 
GCTCTTTTGG AGTCCAAGAT AGTACGCAAA ATCAACTCCT ACACCGATAT TACCTATCAG 
GTATCCGTGG GCGGAGGAGG TGGCATGGTG AAGAGCCGCG ACTTCGTGAA CTTGCGGTCT 
TGTAGGCTCT TTTACAATGG TCAAATCTGC GATGACGATG AGACGGCTCA GCTCAGCAGC 
GATGATGGGA ACAGCAGTCT AAATCGGTCT TGCGAGGGTA GTGTTAGTAC CATTTCCGAT 
GGTGACTCAA ACACCCCACT GCTGCCCAGT AGCGTGTCTA GTTGCAAGGC AACGTTTCCC 
ACTTCATCCA AGGGAGCCGC TATGCCTTTT GACACCCTGG GCAACAGCTT GGGCGCCAAG 
AGCCTAGGTC CCATCGTGAA CTTTGACGAG GAGCCACCGC CATTGGATCA GGACGAGTTC 
GAGGATGCTA AGGACAAGGT CGACGGCGAA GCGAATAACA TGACGAAACC AAATGTACCC 
AGCGTGGGAA AAACCAAGGA CAGGGTTTGG GTCACTTCGG CGGTTAGTGT GCAATATGCT 
GCCGTTCCAC CTTCACCCAA ATACACAAGA GGGCAGAACA TTGTCAGTGG GTTTGCGTTT 
CGTGAAATCG TTGGAAAATC GGATAGCTGC ATTGTGGAGT GGGTGCTTTG CCTAGACCTA 
AAAGGCTATA TTCCTCGCTA TGTCCTGGAT GCAGCCCTCA CCTCGTCCAT GACCGACTAC 
ATTAGCAATC TGCGCAAGCA TGTCAACGAA CTGAGGCAGA AGGGGCGTGG TCGAGCCCCC 
AGGACCCACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286848.1
CDS	27..1058
Translation	MTSDDEYTQMGLDCLRKAYEIIESSDWKVEKVNQKGDTIHSTQRDKIGKIYKLTARIKYPAKALMEDLFYRIEDCPKWNPALLESKIVRKINSYTDITYQVSVGGGGGMVKSRDFVNLRSCRLFYNGQICDDDETAQLSSDDGNSSLNRSCEGSVSTISDGDSNTPLLPSSVSSCKATFPTSSKGAAMPFDTLGNSLGAKSLGPIVNFDEEPPPLDQDEFEDAKDKVDGEANNMTKPNVPSVGKTKDRVWVTSAVSVQYAAVPPSPKYTRGQNIVSGFAFREIVGKSDSCIVEWVLCLDLKGYIPRYVLDAALTSSMTDYISNLRKHVNELRQKGRGRAPRTH

Target ORF information:

RefSeq Version	NM_001299919.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Start1, transcript variant D (Start1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299919.1

ORF Insert Sequence:

ATGACATCGG ATGATGAGTA CACACAAATG GGATTGGATT GCCTTCGAAA GGCCTACGAG 
ATCATCGAGT CAAGTGACTG GAAGGTGGAA AAAGTTAACC AGAAAGGCGA CACCATACAC 
AGCACTCAGC GCGACAAGAT TGGAAAGATC TACAAGTTGA CGGCCCGCAT CAAGTATCCT 
GCAAAGGCTC TGATGGAAGA TCTGTTCTAT CGCATTGAAG ACTGTCCCAA GTGGAATCCT 
GCTCTTTTGG AGTCCAAGAT AGTACGCAAA ATCAACTCCT ACACCGATAT TACCTATCAG 
GTATCCGTGG GCGGAGGAGG TGGCATGGTG AAGAGCCGCG ACTTCGTGAA CTTGCGGTCT 
TGTAGGCTCT TTTACAATGG TCAAATCTGC GATGACGATG AGACGGCTCA GCTCAGCAGC 
GATGATGGGA ACAGCAGTCT AAATCGGTCT TGCGAGGGTA GTGTTAGTAC CATTTCCGAT 
GGTGACTCAA ACACCCCACT GCTGCCCAGT AGCGTGTCTA GTTGCAAGGC AACGTTTCCC 
ACTTCATCCA AGGGAGCCGC TATGCCTTTT GACACCCTGG GCAACAGCTT GGGCGCCAAG 
AGCCTAGGTC CCATCGTGAA CTTTGACGAG GAGCCACCGC CATTGGATCA GGACGAGTTC 
GAGGATGCTA AGGACAAGGT CGACGGCGAA GCGAATAACA TGACGAAACC AAATGTACCC 
AGCGTGGGAA AAACCAAGGA CAGGGTTTGG GTCACTTCGG CGGTTAGTGT GCAATATGCT 
GCCGTTCCAC CTTCACCCAA ATACACAAGA GGGCAGAACA TTGTCAGTGG GTTTGCGTTT 
CGTGAAATCG TTGGAAAATC GGATAGCTGC ATTGTGGAGT GGGTGCTTTG CCTAGACCTA 
AAAGGCTATA TTCCTCGCTA TGTCCTGGAT GCAGCCCTCA CCTCGTCCAT GACCGACTAC 
ATTAGCAATC TGCGCAAGCA TGTCAACGAA CTGAGGCAGA AGGGGCGTGG TCGAGCCCCC 
AGGACCCACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Start1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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