SerT cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	SerT
Entrez Gene ID	37895
Full Name	Serotonin transporter
Synonyms	CG4545,DmSERT,DmSerT,Dmel\CG4545,SERT,Sert,dSERT,dSERT1,dSerT,dSert,dmSERT,dsert,serT
General protein information	Preferred Names Serotonin transporter Names CG4545 gene product from transcript CG4545-RB CG4545-PA CG4545-PB SerT-PA SerT-PB cocaine-sensitive serotonin transporter drosophila serotonin transporter serotonin transporter
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 60C8-60C8

mRNA and Protein(s)

mRNA	Protein	Name
NM_079122.3	NP_523846.2	serotonin transporter, isoform A
NM_001299910.1	NP_001286839.1	serotonin transporter, isoform B

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010621	XP_310113.4
Xenopus tropicalis (tropical clawed frog)	LOC100496361	XP_002932130.2
Gallus gallus (chicken)	LOC427700	XP_425275.3
Caenorhabditis elegans (roundworm)	mod-5	NP_491095.3
Danio rerio (zebrafish)	slc6a4b	NP_001170930.1
Drosophila melanogaster (fruit fly)	SerT	NP_523846.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that functional differences between human, and Drosophila melanogaster, and Caenorhabditis elegans serotonin transporters (SERT) in recognition of MDMA (3,4-methylenedioxy-methamphetamine, "ecstasy") as substrate are due to a single amino acid in transmembrane domain 10 (Glu394 in hSERT; Asn484 in dmSERT; Asp517 in ceSERT).
Title: The external gate of the human and Drosophila serotonin transporters requires a basic/acidic amino acid pair for 3,4-methylenedioxymethamphetamine (MDMA) translocation and the induction of substrate efflux.

These data suggest that a small number of neurons selectively express factor(s) required for 5-HT storage, and potentially for function of dSERT.
Title: Cell-type-specific limitation on in vivo serotonin storage following ectopic expression of the Drosophila serotonin transporter, dSERT.

The following SerT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SerT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11166	NM_001299910.1 Latest version!	Drosophila melanogaster serotonin transporter, transcript variant B (SerT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa11166	NM_079122.3 Latest version!	Drosophila melanogaster serotonin transporter, transcript variant A (SerT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SerT ( NM_079122.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523846.2 Drosophila melanogaster serotonin transporter, transcript variant A (SerT), mRNA.

Price & Availability↑

CloneID	OFa11166
Clone ID Related Accession (Same CDS sequence)	NM_079122.3 , NM_001299910.1
Accession Version	NM_079122.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	serotonin transporter, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_079122.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACCGCA GCGGGAGCTC CGATTTCGCA GGAGCAGCTG CCACGACGGG CCGCTCGAAT 
CCCGCGCCCT GGAGCGACGA CAAAGAGTCG CCAAACAACG AGGACGACTC CAACGAGGAC 
GATGGCGACC ACACGACGCC CGCCAAGGTC ACCGATCCAC TGGCCCCCAA GCTGGCCAAC 
AACGAGCGCA TTCTGGTGGT CTCCGTGACG GAGAGGACTC GCGAGACCTG GGGACAGAAG 
GCGGAGTTCC TCCTGGCCGT GATTGGGTTC GCAGTGGATC TGGGCAATGT GTGGCGCTTC 
CCGTACATCT GCTATCAGAA CGGAGGCGGC GCCTTCCTGG TGCCCTACTG CCTCTTCCTC 
ATCTTCGGTG GACTGCCCCT GTTCTACATG GAGCTGGCGC TGGGCCAGTT CCACCGATGT 
GGTTGCCTCA GCATCTGGAA GCGCATCTGT CCGGCCCTAA AAGGCGTGGG CTATGCGATC 
TGCCTAATCG ACATTTATAT GGGCATGTAC TACAACACGA TTATCGGCTG GGCGGTATAC 
TACCTCTTCG CCTCGTTCAC ATCCAAGTTG CCGTGGACGT CGTGCGATAA TCCCTGGAAC 
ACTGAGAACT GCATGCAGGT GACCAGTGAG AACTTCACGG AACTGGCCAC GTCGCCGGCC 
AAGGAGTTCT TTGAGCGAAA GGTTTTGGAG AGCTACAAGG GCAACGGGCT GGACTTCATG 
GGTCCGGTGA AGCCCACGCT GGCACTCTGC GTCTTCGGGG TCTTTGTCCT GGTCTACTTC 
TCCCTGTGGA AGGGAGTGCG CAGTGCCGGA AAGGTGGTGT GGGTCACCGC TCTGGCTCCT 
TATGTGGTGC TAATCATCCT GCTGGTTAGA GGAGTTTCCC TGCCCGGCGC GGACGAGGGC 
ATCAAGTACT ACCTAACCCC GGAGTGGCAC AAACTGAAGA ACTCCAAAGT CTGGATCGAC 
GCCGCATCCC AGATTTTCTT CTCCCTTGGC CCCGGATTCG GAACCCTGCT GGCCCTGTCC 
AGCTACAACA AGTTCAACAA CAACTGCTAT CGCGATGCTC TCATCACCAG CAGCATCAAC 
TGCTTGACCA GTTTCCTGGC CGGCTTTGTC ATCTTCTCCG TTTTGGGGTA CATGGCCTAC 
GTCCAAAAGA CCTCCATCGA CAAGGTGGGC CTGGAGGGTC CGGGGCTGGT GTTCATCGTT 
TACCCGGAGG CCATAGCCAC GATGAGTGGC TCGGTGTTCT GGAGCATCAT CTTCTTCCTG 
ATGCTGATTA CCCTGGGACT GGACAGCACC TTTGGCGGAT TGGAGGCGAT GATAACAGCG 
CTGTGCGACG AGTATCCGCG AGTGATTGGC AGGCGCAGGG AGCTGTTCGT CCTGCTGCTC 
CTGGCTTTCA TCTTCCTGTG CGCCCTTCCC ACGATGACCT ACGGTGGCGT GGTGCTGGTC 
AACTTCCTGA ATGTCTACGG ACCCGGGTTG GCCATCCTCT TTGTGGTCTT CGTGGAGGCA 
GCCGGAGTCT TTTGGTTCTA CGGAGTGGAC CGCTTCAGCT CGGATGTGGA ACAGATGCTG 
GGCAGCAAGC CAGGCTTATT CTGGCGGATC TGCTGGACGT ACATCAGCCC TGTGTTCCTG 
CTGACCATAT TCATTTTCTC CATCATGGGC TACAAGGAGA TGCTCGGCGA GGAGTACTAC 
TACCCGGACT GGAGCTACCA GGTGGGCTGG GCGGTCACCT GCTCGTCGGT GCTCTGCATC 
CCCATGTACA TTATCTACAA GTTCTTCTTC GCCTCGAAAG GCGGCTGCCG CCAGAGGCTG 
CAGGAGTCCT TCCAGCCGGA GGACAACTGC GGATCGGTGG TGCCCGGCCA ACAGGGCACC 
TCGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523846.2
CDS	458..2326
Translation	MDRSGSSDFAGAAATTGRSNPAPWSDDKESPNNEDDSNEDDGDHTTPAKVTDPLAPKLANNERILVVSVTERTRETWGQKAEFLLAVIGFAVDLGNVWRFPYICYQNGGGAFLVPYCLFLIFGGLPLFYMELALGQFHRCGCLSIWKRICPALKGVGYAICLIDIYMGMYYNTIIGWAVYYLFASFTSKLPWTSCDNPWNTENCMQVTSENFTELATSPAKEFFERKVLESYKGNGLDFMGPVKPTLALCVFGVFVLVYFSLWKGVRSAGKVVWVTALAPYVVLIILLVRGVSLPGADEGIKYYLTPEWHKLKNSKVWIDAASQIFFSLGPGFGTLLALSSYNKFNNNCYRDALITSSINCLTSFLAGFVIFSVLGYMAYVQKTSIDKVGLEGPGLVFIVYPEAIATMSGSVFWSIIFFLMLITLGLDSTFGGLEAMITALCDEYPRVIGRRRELFVLLLLAFIFLCALPTMTYGGVVLVNFLNVYGPGLAILFVVFVEAAGVFWFYGVDRFSSDVEQMLGSKPGLFWRICWTYISPVFLLTIFIFSIMGYKEMLGEEYYYPDWSYQVGWAVTCSSVLCIPMYIIYKFFFASKGGCRQRLQESFQPEDNCGSVVPGQQGTSV

Target ORF information:

RefSeq Version	NM_079122.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster serotonin transporter, transcript variant A (SerT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079122.3

ORF Insert Sequence:

ATGGACCGCA GCGGGAGCTC CGATTTCGCA GGAGCAGCTG CCACGACGGG CCGCTCGAAT 
CCCGCGCCCT GGAGCGACGA CAAAGAGTCG CCAAACAACG AGGACGACTC CAACGAGGAC 
GATGGCGACC ACACGACGCC CGCCAAGGTC ACCGATCCAC TGGCCCCCAA GCTGGCCAAC 
AACGAGCGCA TTCTGGTGGT CTCCGTGACG GAGAGGACTC GCGAGACCTG GGGACAGAAG 
GCGGAGTTCC TCCTGGCCGT GATTGGGTTC GCAGTGGATC TGGGCAATGT GTGGCGCTTC 
CCGTACATCT GCTATCAGAA CGGAGGCGGC GCCTTCCTGG TGCCCTACTG CCTCTTCCTC 
ATCTTCGGTG GACTGCCCCT GTTCTACATG GAGCTGGCGC TGGGCCAGTT CCACCGATGT 
GGTTGCCTCA GCATCTGGAA GCGCATCTGT CCGGCCCTAA AAGGCGTGGG CTATGCGATC 
TGCCTAATCG ACATTTATAT GGGCATGTAC TACAACACGA TTATCGGCTG GGCGGTATAC 
TACCTCTTCG CCTCGTTCAC ATCCAAGTTG CCGTGGACGT CGTGCGATAA TCCCTGGAAC 
ACTGAGAACT GCATGCAGGT GACCAGTGAG AACTTCACGG AACTGGCCAC GTCGCCGGCC 
AAGGAGTTCT TTGAGCGAAA GGTTTTGGAG AGCTACAAGG GCAACGGGCT GGACTTCATG 
GGTCCGGTGA AGCCCACGCT GGCACTCTGC GTCTTCGGGG TCTTTGTCCT GGTCTACTTC 
TCCCTGTGGA AGGGAGTGCG CAGTGCCGGA AAGGTGGTGT GGGTCACCGC TCTGGCTCCT 
TATGTGGTGC TAATCATCCT GCTGGTTAGA GGAGTTTCCC TGCCCGGCGC GGACGAGGGC 
ATCAAGTACT ACCTAACCCC GGAGTGGCAC AAACTGAAGA ACTCCAAAGT CTGGATCGAC 
GCCGCATCCC AGATTTTCTT CTCCCTTGGC CCCGGATTCG GAACCCTGCT GGCCCTGTCC 
AGCTACAACA AGTTCAACAA CAACTGCTAT CGCGATGCTC TCATCACCAG CAGCATCAAC 
TGCTTGACCA GTTTCCTGGC CGGCTTTGTC ATCTTCTCCG TTTTGGGGTA CATGGCCTAC 
GTCCAAAAGA CCTCCATCGA CAAGGTGGGC CTGGAGGGTC CGGGGCTGGT GTTCATCGTT 
TACCCGGAGG CCATAGCCAC GATGAGTGGC TCGGTGTTCT GGAGCATCAT CTTCTTCCTG 
ATGCTGATTA CCCTGGGACT GGACAGCACC TTTGGCGGAT TGGAGGCGAT GATAACAGCG 
CTGTGCGACG AGTATCCGCG AGTGATTGGC AGGCGCAGGG AGCTGTTCGT CCTGCTGCTC 
CTGGCTTTCA TCTTCCTGTG CGCCCTTCCC ACGATGACCT ACGGTGGCGT GGTGCTGGTC 
AACTTCCTGA ATGTCTACGG ACCCGGGTTG GCCATCCTCT TTGTGGTCTT CGTGGAGGCA 
GCCGGAGTCT TTTGGTTCTA CGGAGTGGAC CGCTTCAGCT CGGATGTGGA ACAGATGCTG 
GGCAGCAAGC CAGGCTTATT CTGGCGGATC TGCTGGACGT ACATCAGCCC TGTGTTCCTG 
CTGACCATAT TCATTTTCTC CATCATGGGC TACAAGGAGA TGCTCGGCGA GGAGTACTAC 
TACCCGGACT GGAGCTACCA GGTGGGCTGG GCGGTCACCT GCTCGTCGGT GCTCTGCATC 
CCCATGTACA TTATCTACAA GTTCTTCTTC GCCTCGAAAG GCGGCTGCCG CCAGAGGCTG 
CAGGAGTCCT TCCAGCCGGA GGACAACTGC GGATCGGTGG TGCCCGGCCA ACAGGGCACC 
TCGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SerT ( NM_001299910.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286839.1 Drosophila melanogaster serotonin transporter, transcript variant B (SerT), mRNA.

Price & Availability↑

CloneID	OFa11166
Clone ID Related Accession (Same CDS sequence)	NM_079122.3 , NM_001299910.1
Accession Version	NM_001299910.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	serotonin transporter, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACCGCA GCGGGAGCTC CGATTTCGCA GGAGCAGCTG CCACGACGGG CCGCTCGAAT 
CCCGCGCCCT GGAGCGACGA CAAAGAGTCG CCAAACAACG AGGACGACTC CAACGAGGAC 
GATGGCGACC ACACGACGCC CGCCAAGGTC ACCGATCCAC TGGCCCCCAA GCTGGCCAAC 
AACGAGCGCA TTCTGGTGGT CTCCGTGACG GAGAGGACTC GCGAGACCTG GGGACAGAAG 
GCGGAGTTCC TCCTGGCCGT GATTGGGTTC GCAGTGGATC TGGGCAATGT GTGGCGCTTC 
CCGTACATCT GCTATCAGAA CGGAGGCGGC GCCTTCCTGG TGCCCTACTG CCTCTTCCTC 
ATCTTCGGTG GACTGCCCCT GTTCTACATG GAGCTGGCGC TGGGCCAGTT CCACCGATGT 
GGTTGCCTCA GCATCTGGAA GCGCATCTGT CCGGCCCTAA AAGGCGTGGG CTATGCGATC 
TGCCTAATCG ACATTTATAT GGGCATGTAC TACAACACGA TTATCGGCTG GGCGGTATAC 
TACCTCTTCG CCTCGTTCAC ATCCAAGTTG CCGTGGACGT CGTGCGATAA TCCCTGGAAC 
ACTGAGAACT GCATGCAGGT GACCAGTGAG AACTTCACGG AACTGGCCAC GTCGCCGGCC 
AAGGAGTTCT TTGAGCGAAA GGTTTTGGAG AGCTACAAGG GCAACGGGCT GGACTTCATG 
GGTCCGGTGA AGCCCACGCT GGCACTCTGC GTCTTCGGGG TCTTTGTCCT GGTCTACTTC 
TCCCTGTGGA AGGGAGTGCG CAGTGCCGGA AAGGTGGTGT GGGTCACCGC TCTGGCTCCT 
TATGTGGTGC TAATCATCCT GCTGGTTAGA GGAGTTTCCC TGCCCGGCGC GGACGAGGGC 
ATCAAGTACT ACCTAACCCC GGAGTGGCAC AAACTGAAGA ACTCCAAAGT CTGGATCGAC 
GCCGCATCCC AGATTTTCTT CTCCCTTGGC CCCGGATTCG GAACCCTGCT GGCCCTGTCC 
AGCTACAACA AGTTCAACAA CAACTGCTAT CGCGATGCTC TCATCACCAG CAGCATCAAC 
TGCTTGACCA GTTTCCTGGC CGGCTTTGTC ATCTTCTCCG TTTTGGGGTA CATGGCCTAC 
GTCCAAAAGA CCTCCATCGA CAAGGTGGGC CTGGAGGGTC CGGGGCTGGT GTTCATCGTT 
TACCCGGAGG CCATAGCCAC GATGAGTGGC TCGGTGTTCT GGAGCATCAT CTTCTTCCTG 
ATGCTGATTA CCCTGGGACT GGACAGCACC TTTGGCGGAT TGGAGGCGAT GATAACAGCG 
CTGTGCGACG AGTATCCGCG AGTGATTGGC AGGCGCAGGG AGCTGTTCGT CCTGCTGCTC 
CTGGCTTTCA TCTTCCTGTG CGCCCTTCCC ACGATGACCT ACGGTGGCGT GGTGCTGGTC 
AACTTCCTGA ATGTCTACGG ACCCGGGTTG GCCATCCTCT TTGTGGTCTT CGTGGAGGCA 
GCCGGAGTCT TTTGGTTCTA CGGAGTGGAC CGCTTCAGCT CGGATGTGGA ACAGATGCTG 
GGCAGCAAGC CAGGCTTATT CTGGCGGATC TGCTGGACGT ACATCAGCCC TGTGTTCCTG 
CTGACCATAT TCATTTTCTC CATCATGGGC TACAAGGAGA TGCTCGGCGA GGAGTACTAC 
TACCCGGACT GGAGCTACCA GGTGGGCTGG GCGGTCACCT GCTCGTCGGT GCTCTGCATC 
CCCATGTACA TTATCTACAA GTTCTTCTTC GCCTCGAAAG GCGGCTGCCG CCAGAGGCTG 
CAGGAGTCCT TCCAGCCGGA GGACAACTGC GGATCGGTGG TGCCCGGCCA ACAGGGCACC 
TCGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286839.1
CDS	1000..2868
Translation	MDRSGSSDFAGAAATTGRSNPAPWSDDKESPNNEDDSNEDDGDHTTPAKVTDPLAPKLANNERILVVSVTERTRETWGQKAEFLLAVIGFAVDLGNVWRFPYICYQNGGGAFLVPYCLFLIFGGLPLFYMELALGQFHRCGCLSIWKRICPALKGVGYAICLIDIYMGMYYNTIIGWAVYYLFASFTSKLPWTSCDNPWNTENCMQVTSENFTELATSPAKEFFERKVLESYKGNGLDFMGPVKPTLALCVFGVFVLVYFSLWKGVRSAGKVVWVTALAPYVVLIILLVRGVSLPGADEGIKYYLTPEWHKLKNSKVWIDAASQIFFSLGPGFGTLLALSSYNKFNNNCYRDALITSSINCLTSFLAGFVIFSVLGYMAYVQKTSIDKVGLEGPGLVFIVYPEAIATMSGSVFWSIIFFLMLITLGLDSTFGGLEAMITALCDEYPRVIGRRRELFVLLLLAFIFLCALPTMTYGGVVLVNFLNVYGPGLAILFVVFVEAAGVFWFYGVDRFSSDVEQMLGSKPGLFWRICWTYISPVFLLTIFIFSIMGYKEMLGEEYYYPDWSYQVGWAVTCSSVLCIPMYIIYKFFFASKGGCRQRLQESFQPEDNCGSVVPGQQGTSV

Target ORF information:

RefSeq Version	NM_001299910.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster serotonin transporter, transcript variant B (SerT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299910.1

ORF Insert Sequence:

ATGGACCGCA GCGGGAGCTC CGATTTCGCA GGAGCAGCTG CCACGACGGG CCGCTCGAAT 
CCCGCGCCCT GGAGCGACGA CAAAGAGTCG CCAAACAACG AGGACGACTC CAACGAGGAC 
GATGGCGACC ACACGACGCC CGCCAAGGTC ACCGATCCAC TGGCCCCCAA GCTGGCCAAC 
AACGAGCGCA TTCTGGTGGT CTCCGTGACG GAGAGGACTC GCGAGACCTG GGGACAGAAG 
GCGGAGTTCC TCCTGGCCGT GATTGGGTTC GCAGTGGATC TGGGCAATGT GTGGCGCTTC 
CCGTACATCT GCTATCAGAA CGGAGGCGGC GCCTTCCTGG TGCCCTACTG CCTCTTCCTC 
ATCTTCGGTG GACTGCCCCT GTTCTACATG GAGCTGGCGC TGGGCCAGTT CCACCGATGT 
GGTTGCCTCA GCATCTGGAA GCGCATCTGT CCGGCCCTAA AAGGCGTGGG CTATGCGATC 
TGCCTAATCG ACATTTATAT GGGCATGTAC TACAACACGA TTATCGGCTG GGCGGTATAC 
TACCTCTTCG CCTCGTTCAC ATCCAAGTTG CCGTGGACGT CGTGCGATAA TCCCTGGAAC 
ACTGAGAACT GCATGCAGGT GACCAGTGAG AACTTCACGG AACTGGCCAC GTCGCCGGCC 
AAGGAGTTCT TTGAGCGAAA GGTTTTGGAG AGCTACAAGG GCAACGGGCT GGACTTCATG 
GGTCCGGTGA AGCCCACGCT GGCACTCTGC GTCTTCGGGG TCTTTGTCCT GGTCTACTTC 
TCCCTGTGGA AGGGAGTGCG CAGTGCCGGA AAGGTGGTGT GGGTCACCGC TCTGGCTCCT 
TATGTGGTGC TAATCATCCT GCTGGTTAGA GGAGTTTCCC TGCCCGGCGC GGACGAGGGC 
ATCAAGTACT ACCTAACCCC GGAGTGGCAC AAACTGAAGA ACTCCAAAGT CTGGATCGAC 
GCCGCATCCC AGATTTTCTT CTCCCTTGGC CCCGGATTCG GAACCCTGCT GGCCCTGTCC 
AGCTACAACA AGTTCAACAA CAACTGCTAT CGCGATGCTC TCATCACCAG CAGCATCAAC 
TGCTTGACCA GTTTCCTGGC CGGCTTTGTC ATCTTCTCCG TTTTGGGGTA CATGGCCTAC 
GTCCAAAAGA CCTCCATCGA CAAGGTGGGC CTGGAGGGTC CGGGGCTGGT GTTCATCGTT 
TACCCGGAGG CCATAGCCAC GATGAGTGGC TCGGTGTTCT GGAGCATCAT CTTCTTCCTG 
ATGCTGATTA CCCTGGGACT GGACAGCACC TTTGGCGGAT TGGAGGCGAT GATAACAGCG 
CTGTGCGACG AGTATCCGCG AGTGATTGGC AGGCGCAGGG AGCTGTTCGT CCTGCTGCTC 
CTGGCTTTCA TCTTCCTGTG CGCCCTTCCC ACGATGACCT ACGGTGGCGT GGTGCTGGTC 
AACTTCCTGA ATGTCTACGG ACCCGGGTTG GCCATCCTCT TTGTGGTCTT CGTGGAGGCA 
GCCGGAGTCT TTTGGTTCTA CGGAGTGGAC CGCTTCAGCT CGGATGTGGA ACAGATGCTG 
GGCAGCAAGC CAGGCTTATT CTGGCGGATC TGCTGGACGT ACATCAGCCC TGTGTTCCTG 
CTGACCATAT TCATTTTCTC CATCATGGGC TACAAGGAGA TGCTCGGCGA GGAGTACTAC 
TACCCGGACT GGAGCTACCA GGTGGGCTGG GCGGTCACCT GCTCGTCGGT GCTCTGCATC 
CCCATGTACA TTATCTACAA GTTCTTCTTC GCCTCGAAAG GCGGCTGCCG CCAGAGGCTG 
CAGGAGTCCT TCCAGCCGGA GGACAACTGC GGATCGGTGG TGCCCGGCCA ACAGGGCACC 
TCGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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SerT cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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