Rpn8 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rpn8
Entrez Gene ID	37894
Full Name	Regulatory particle non-ATPase 8
Synonyms	CG3416,Dmel\CG3416,Mov34,P26270,PRSC_DROME,PSMD7,RPN8,S12,l(2)k08003,p39B
General protein information	Preferred Names Regulatory particle non-ATPase 8 Names CG3416 gene product from transcript CG3416-RC CG3416-PA CG3416-PB CG3416-PC Mov34 Rpn8-PA Rpn8-PB Rpn8-PC regulatory particle non-ATPase 8
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 60C8-60C8

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274238.1	NP_001261167.1	regulatory particle non-ATPase 8, isoform B
NM_001274239.1	NP_001261168.1	regulatory particle non-ATPase 8, isoform C
NM_079121.3	NP_523845.2	regulatory particle non-ATPase 8, isoform A

Pathways from BioSystems

Homologies

Homo sapiens (human)	PSMD7	NP_002802.2
	PSMD7	XP_001102147.1
Eremothecium gossypii	AGOS_AAL116W	NP_982426.1
Canis lupus familiaris (dog)	PSMD7	XP_536802.1
Bos taurus (cattle)	PSMD7	NP_001029595.1
Rattus norvegicus (Norway rat)	Psmd7	NP_001100896.1
Schizosaccharomyces pombe (fission yeast)	rpn8	NP_587803.1
Arabidopsis thaliana (thale cress)	RPN8A	NP_196197.1
Danio rerio (zebrafish)	psmd7	NP_956083.1
Saccharomyces cerevisiae (baker's yeast)	RPN8	NP_014904.3
Kluyveromyces lactis	KLLA0C09526g	XP_452622.1
Magnaporthe oryzae (rice blast fungus)	MGG_01059	XP_003717880.1
Neurospora crassa	NCU01547	XP_956250.1
Oryza sativa (rice)	Os04g0661900	NP_001054152.1
Gallus gallus (chicken)	PSMD7	XP_414229.4
Drosophila melanogaster (fruit fly)	Mov34	NP_001261168.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012135	XP_320392.4
Arabidopsis thaliana (thale cress)	MEE34	NP_187736.1
Xenopus tropicalis (tropical clawed frog)	psmd7	NP_001032349.1
Pan troglodytes (chimpanzee)	PSMD7	XP_511103.2
Mus musculus (house mouse)	Psmd7	NP_034947.1
Caenorhabditis elegans (roundworm)	rpn-8	NP_491319.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rpn8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rpn8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa11165	NM_079121.3 Latest version!	Drosophila melanogaster regulatory particle non-ATPase 8, transcript variant A (Rpn8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa11165	NM_001274239.1 Latest version!	Drosophila melanogaster regulatory particle non-ATPase 8, transcript variant C (Rpn8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa13235	NM_001274238.1 Latest version!	Drosophila melanogaster regulatory particle non-ATPase 8, transcript variant B (Rpn8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rpn8 ( NM_001274239.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261168.1 Drosophila melanogaster regulatory particle non-ATPase 8, transcript variant C (Rpn8), mRNA.

Price & Availability↑

CloneID	OFa11165
Clone ID Related Accession (Same CDS sequence)	NM_001274239.1 , NM_079121.3
Accession Version	NM_001274239.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	regulatory particle non-ATPase 8, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGTCGC AGGAGGTGAG CGTAAACAAA GTGATAGTGC ATCCATTGGT TCTGCTGTCC 
GTGGTGGATC ACTTCAACCG GATGGGAAAG ATTGGCAACC AGAAGCGCGT AGTGGGAGTC 
CTTCTGGGCT GCTGGCGATC CAAGGGAGTG CTCGATGTGT CCAACAGCTT CGCAGTGCCC 
TTCGACGAGG ATGACAAGGA CAAGTCGGTG TGGTTCCTGG ACCACGACTA CCTGGAGAAT 
ATGTACGGCA TGTTCAAAAA GGTGAACGCC AGGGAACGGG TTGTGGGATG GTATCACACA 
GGTCCCAAGC TCCACCAAAA CGACATAGCC ATCAACGAGC TGGTCCGGCG CTATTGTCCC 
AACTCCGTGC TGGTCATCAT CGACGCCAAG CCCAAGGATT TGGGCCTGCC CACAGAGGCG 
TACATATCGG TGGAGGAAGT CCATGACGAC GGCTCTCCGA CCAGCAAAAC TTTCGAGCAT 
GTGCCCAGCG AGATTGGCGC CGAGGAGGCG GAGGAGGTGG GCGTGGAGCA TCTGCTGCGT 
GACATCAAGG ACACAACCGT GGGCAGCCTG TCGCAAAAGA TCACCAACCA GCTCATGGGT 
CTGAAAGGCC TGAATGCCCA GCTGCGGGAC ATAAAGCAGT ATCTGCAGCG CGTCGGCGAC 
AGCAAGATGC CAATCAACCA CCAGATTGTC TACCAGCTGC AGGACATCTT CAACCTGCTG 
CCCGATATAA CCAATGACCA GTTCACGGGC ACAATGTATG TGAAGACCAA CGACCAAATG 
CTAGTCGTCT ACCTGGCCTC CATGGTGCGC TCGATCATTG CCCTGCACAA CCTGATCAAC 
AACAAGCTGG CCAACCGCGA CGCCGAGGAG GGCAAGAGCG ACAGCAAGGA GGCCAAGGAG 
AAGAATAAGG ATAGCAAGGA TAAGGACAAC AAGGAGACCA AGGACAAGGA CGGCAAGAAG 
GCGGAGGAGA AGGCCGACAA GGGCAAGGAC GAAGGCGGCA AGGGTTCGCG CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261168.1
CDS	203..1219
Translation	MPSQEVSVNKVIVHPLVLLSVVDHFNRMGKIGNQKRVVGVLLGCWRSKGVLDVSNSFAVPFDEDDKDKSVWFLDHDYLENMYGMFKKVNARERVVGWYHTGPKLHQNDIAINELVRRYCPNSVLVIIDAKPKDLGLPTEAYISVEEVHDDGSPTSKTFEHVPSEIGAEEAEEVGVEHLLRDIKDTTVGSLSQKITNQLMGLKGLNAQLRDIKQYLQRVGDSKMPINHQIVYQLQDIFNLLPDITNDQFTGTMYVKTNDQMLVVYLASMVRSIIALHNLINNKLANRDAEEGKSDSKEAKEKNKDSKDKDNKETKDKDGKKAEEKADKGKDEGGKGSRK

Target ORF information:

RefSeq Version	NM_001274239.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster regulatory particle non-ATPase 8, transcript variant C (Rpn8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274239.1

ORF Insert Sequence:

ATGCCGTCGC AGGAGGTGAG CGTAAACAAA GTGATAGTGC ATCCATTGGT TCTGCTGTCC 
GTGGTGGATC ACTTCAACCG GATGGGAAAG ATTGGCAACC AGAAGCGCGT AGTGGGAGTC 
CTTCTGGGCT GCTGGCGATC CAAGGGAGTG CTCGATGTGT CCAACAGCTT CGCAGTGCCC 
TTCGACGAGG ATGACAAGGA CAAGTCGGTG TGGTTCCTGG ACCACGACTA CCTGGAGAAT 
ATGTACGGCA TGTTCAAAAA GGTGAACGCC AGGGAACGGG TTGTGGGATG GTATCACACA 
GGTCCCAAGC TCCACCAAAA CGACATAGCC ATCAACGAGC TGGTCCGGCG CTATTGTCCC 
AACTCCGTGC TGGTCATCAT CGACGCCAAG CCCAAGGATT TGGGCCTGCC CACAGAGGCG 
TACATATCGG TGGAGGAAGT CCATGACGAC GGCTCTCCGA CCAGCAAAAC TTTCGAGCAT 
GTGCCCAGCG AGATTGGCGC CGAGGAGGCG GAGGAGGTGG GCGTGGAGCA TCTGCTGCGT 
GACATCAAGG ACACAACCGT GGGCAGCCTG TCGCAAAAGA TCACCAACCA GCTCATGGGT 
CTGAAAGGCC TGAATGCCCA GCTGCGGGAC ATAAAGCAGT ATCTGCAGCG CGTCGGCGAC 
AGCAAGATGC CAATCAACCA CCAGATTGTC TACCAGCTGC AGGACATCTT CAACCTGCTG 
CCCGATATAA CCAATGACCA GTTCACGGGC ACAATGTATG TGAAGACCAA CGACCAAATG 
CTAGTCGTCT ACCTGGCCTC CATGGTGCGC TCGATCATTG CCCTGCACAA CCTGATCAAC 
AACAAGCTGG CCAACCGCGA CGCCGAGGAG GGCAAGAGCG ACAGCAAGGA GGCCAAGGAG 
AAGAATAAGG ATAGCAAGGA TAAGGACAAC AAGGAGACCA AGGACAAGGA CGGCAAGAAG 
GCGGAGGAGA AGGCCGACAA GGGCAAGGAC GAAGGCGGCA AGGGTTCGCG CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rpn8 ( NM_079121.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523845.2 Drosophila melanogaster regulatory particle non-ATPase 8, transcript variant A (Rpn8), mRNA.

Price & Availability↑

CloneID	OFa11165
Clone ID Related Accession (Same CDS sequence)	NM_001274239.1 , NM_079121.3
Accession Version	NM_079121.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	regulatory particle non-ATPase 8, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_079121.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGTCGC AGGAGGTGAG CGTAAACAAA GTGATAGTGC ATCCATTGGT TCTGCTGTCC 
GTGGTGGATC ACTTCAACCG GATGGGAAAG ATTGGCAACC AGAAGCGCGT AGTGGGAGTC 
CTTCTGGGCT GCTGGCGATC CAAGGGAGTG CTCGATGTGT CCAACAGCTT CGCAGTGCCC 
TTCGACGAGG ATGACAAGGA CAAGTCGGTG TGGTTCCTGG ACCACGACTA CCTGGAGAAT 
ATGTACGGCA TGTTCAAAAA GGTGAACGCC AGGGAACGGG TTGTGGGATG GTATCACACA 
GGTCCCAAGC TCCACCAAAA CGACATAGCC ATCAACGAGC TGGTCCGGCG CTATTGTCCC 
AACTCCGTGC TGGTCATCAT CGACGCCAAG CCCAAGGATT TGGGCCTGCC CACAGAGGCG 
TACATATCGG TGGAGGAAGT CCATGACGAC GGCTCTCCGA CCAGCAAAAC TTTCGAGCAT 
GTGCCCAGCG AGATTGGCGC CGAGGAGGCG GAGGAGGTGG GCGTGGAGCA TCTGCTGCGT 
GACATCAAGG ACACAACCGT GGGCAGCCTG TCGCAAAAGA TCACCAACCA GCTCATGGGT 
CTGAAAGGCC TGAATGCCCA GCTGCGGGAC ATAAAGCAGT ATCTGCAGCG CGTCGGCGAC 
AGCAAGATGC CAATCAACCA CCAGATTGTC TACCAGCTGC AGGACATCTT CAACCTGCTG 
CCCGATATAA CCAATGACCA GTTCACGGGC ACAATGTATG TGAAGACCAA CGACCAAATG 
CTAGTCGTCT ACCTGGCCTC CATGGTGCGC TCGATCATTG CCCTGCACAA CCTGATCAAC 
AACAAGCTGG CCAACCGCGA CGCCGAGGAG GGCAAGAGCG ACAGCAAGGA GGCCAAGGAG 
AAGAATAAGG ATAGCAAGGA TAAGGACAAC AAGGAGACCA AGGACAAGGA CGGCAAGAAG 
GCGGAGGAGA AGGCCGACAA GGGCAAGGAC GAAGGCGGCA AGGGTTCGCG CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523845.2
CDS	186..1202
Translation	MPSQEVSVNKVIVHPLVLLSVVDHFNRMGKIGNQKRVVGVLLGCWRSKGVLDVSNSFAVPFDEDDKDKSVWFLDHDYLENMYGMFKKVNARERVVGWYHTGPKLHQNDIAINELVRRYCPNSVLVIIDAKPKDLGLPTEAYISVEEVHDDGSPTSKTFEHVPSEIGAEEAEEVGVEHLLRDIKDTTVGSLSQKITNQLMGLKGLNAQLRDIKQYLQRVGDSKMPINHQIVYQLQDIFNLLPDITNDQFTGTMYVKTNDQMLVVYLASMVRSIIALHNLINNKLANRDAEEGKSDSKEAKEKNKDSKDKDNKETKDKDGKKAEEKADKGKDEGGKGSRK

Target ORF information:

RefSeq Version	NM_079121.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster regulatory particle non-ATPase 8, transcript variant A (Rpn8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079121.3

ORF Insert Sequence:

ATGCCGTCGC AGGAGGTGAG CGTAAACAAA GTGATAGTGC ATCCATTGGT TCTGCTGTCC 
GTGGTGGATC ACTTCAACCG GATGGGAAAG ATTGGCAACC AGAAGCGCGT AGTGGGAGTC 
CTTCTGGGCT GCTGGCGATC CAAGGGAGTG CTCGATGTGT CCAACAGCTT CGCAGTGCCC 
TTCGACGAGG ATGACAAGGA CAAGTCGGTG TGGTTCCTGG ACCACGACTA CCTGGAGAAT 
ATGTACGGCA TGTTCAAAAA GGTGAACGCC AGGGAACGGG TTGTGGGATG GTATCACACA 
GGTCCCAAGC TCCACCAAAA CGACATAGCC ATCAACGAGC TGGTCCGGCG CTATTGTCCC 
AACTCCGTGC TGGTCATCAT CGACGCCAAG CCCAAGGATT TGGGCCTGCC CACAGAGGCG 
TACATATCGG TGGAGGAAGT CCATGACGAC GGCTCTCCGA CCAGCAAAAC TTTCGAGCAT 
GTGCCCAGCG AGATTGGCGC CGAGGAGGCG GAGGAGGTGG GCGTGGAGCA TCTGCTGCGT 
GACATCAAGG ACACAACCGT GGGCAGCCTG TCGCAAAAGA TCACCAACCA GCTCATGGGT 
CTGAAAGGCC TGAATGCCCA GCTGCGGGAC ATAAAGCAGT ATCTGCAGCG CGTCGGCGAC 
AGCAAGATGC CAATCAACCA CCAGATTGTC TACCAGCTGC AGGACATCTT CAACCTGCTG 
CCCGATATAA CCAATGACCA GTTCACGGGC ACAATGTATG TGAAGACCAA CGACCAAATG 
CTAGTCGTCT ACCTGGCCTC CATGGTGCGC TCGATCATTG CCCTGCACAA CCTGATCAAC 
AACAAGCTGG CCAACCGCGA CGCCGAGGAG GGCAAGAGCG ACAGCAAGGA GGCCAAGGAG 
AAGAATAAGG ATAGCAAGGA TAAGGACAAC AAGGAGACCA AGGACAAGGA CGGCAAGAAG 
GCGGAGGAGA AGGCCGACAA GGGCAAGGAC GAAGGCGGCA AGGGTTCGCG CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rpn8 ( NM_001274238.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261167.1 Drosophila melanogaster regulatory particle non-ATPase 8, transcript variant B (Rpn8), mRNA.

Price & Availability↑

CloneID	OFa13235
Clone ID Related Accession (Same CDS sequence)	NM_001274238.1
Accession Version	NM_001274238.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	regulatory particle non-ATPase 8, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGTCGC AGGAGGTGAG CGTAAACAAA GTGATAGTGC ATCCATTGGT TCTGCTGTCC 
GTGGTGGATC ACTTCAACCG GATGGGAAAG ATTGGCAACC AGAAGCGCGT AGTGGGAGTC 
CTTCTGGGCT GCTGGCGATC CAAGGGAGTG CTCGATGTGT CCAACAGCTT CGCAGTGCCC 
TTCGACGAGG ATGACAAGGA CAAGTCGGTG TGGTTCCTGG ACCACGACTA CCTGGAGAAT 
ATGTACGGCA TGTTCAAAAA GGTGAACGCC AGGGAACGGG TTGTGGGATG GTATCACACA 
GGTCCCAAGC TCCACCAAAA CGACATAGCC ATCAACGAGC TGGTCCGGCG CTATTGTCCC 
AACTCCGTGC TGGTCATCAT CGACGCCAAG CCCAAGGATT TGGGCCTGCC CACAGAGGCG 
TACATATCGG TGGAGGAAGT CCATGACGAC GGCTCTCCGA CCAGCAAAAC TTTCGAGCAT 
GTGCCCAGCG AGATTGGCGC CGAGGAGGCG GAGGAGGTGG GCGTGGAGCA TCTGCTGCGT 
GACATCAAGG ACACAACCGT GGGCAGCCTG TCGCAAAAGA TCACCAACCA GCTCATGGGT 
CTGAAAGGCC TGAATGCCCA GCTGCGGGAC ATAAAGCAGT ATCTGCAGCG CGTCGGCGAC 
AGCAAGATGC CAATCAACCA CCAGATTGTC TACCAGCTGC AGGACATCTT CAACCTGCTG 
CCCGATATAA CCAATGACCA GTTCACGGGC ACAATGTATG TGAAGACCAA CGACCAAATG 
CTAGTCGTCT ACCTGGCCTC CATGGTGCGC TCGATCATTG CCCTGCACAA CCTGATCAAC 
AACAAGCTGG CCAACCGCGA CGCCGAGGAG GGCAAGAGCG ACAGCAAGGA GGCCAAGGAG 
AAGAATAAGG ATAGCAAGGA TAAGGACAAC AAGGAGACCA AGGACAAGGA CGGCAAGAAG 
GCGGAGGAGA AGGCCGACAA GGGCAAGGAC GAAGGCGGCA AGGGTTCGCG CAAATAGGAG 
CGGAAGAAAA AGCGCAAACC CCGTCGCAAA CGAAAGAAGC AGCGGGCGCA GGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261167.1
CDS	186..1262
Translation	MPSQEVSVNKVIVHPLVLLSVVDHFNRMGKIGNQKRVVGVLLGCWRSKGVLDVSNSFAVPFDEDDKDKSVWFLDHDYLENMYGMFKKVNARERVVGWYHTGPKLHQNDIAINELVRRYCPNSVLVIIDAKPKDLGLPTEAYISVEEVHDDGSPTSKTFEHVPSEIGAEEAEEVGVEHLLRDIKDTTVGSLSQKITNQLMGLKGLNAQLRDIKQYLQRVGDSKMPINHQIVYQLQDIFNLLPDITNDQFTGTMYVKTNDQMLVVYLASMVRSIIALHNLINNKLANRDAEEGKSDSKEAKEKNKDSKDKDNKETKDKDGKKAEEKADKGKDEGGKGSRK*ERKKKRKPRRKRKKQRAQD

Target ORF information:

RefSeq Version	NM_001274238.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster regulatory particle non-ATPase 8, transcript variant B (Rpn8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274238.1

ORF Insert Sequence:

ATGCCGTCGC AGGAGGTGAG CGTAAACAAA GTGATAGTGC ATCCATTGGT TCTGCTGTCC 
GTGGTGGATC ACTTCAACCG GATGGGAAAG ATTGGCAACC AGAAGCGCGT AGTGGGAGTC 
CTTCTGGGCT GCTGGCGATC CAAGGGAGTG CTCGATGTGT CCAACAGCTT CGCAGTGCCC 
TTCGACGAGG ATGACAAGGA CAAGTCGGTG TGGTTCCTGG ACCACGACTA CCTGGAGAAT 
ATGTACGGCA TGTTCAAAAA GGTGAACGCC AGGGAACGGG TTGTGGGATG GTATCACACA 
GGTCCCAAGC TCCACCAAAA CGACATAGCC ATCAACGAGC TGGTCCGGCG CTATTGTCCC 
AACTCCGTGC TGGTCATCAT CGACGCCAAG CCCAAGGATT TGGGCCTGCC CACAGAGGCG 
TACATATCGG TGGAGGAAGT CCATGACGAC GGCTCTCCGA CCAGCAAAAC TTTCGAGCAT 
GTGCCCAGCG AGATTGGCGC CGAGGAGGCG GAGGAGGTGG GCGTGGAGCA TCTGCTGCGT 
GACATCAAGG ACACAACCGT GGGCAGCCTG TCGCAAAAGA TCACCAACCA GCTCATGGGT 
CTGAAAGGCC TGAATGCCCA GCTGCGGGAC ATAAAGCAGT ATCTGCAGCG CGTCGGCGAC 
AGCAAGATGC CAATCAACCA CCAGATTGTC TACCAGCTGC AGGACATCTT CAACCTGCTG 
CCCGATATAA CCAATGACCA GTTCACGGGC ACAATGTATG TGAAGACCAA CGACCAAATG 
CTAGTCGTCT ACCTGGCCTC CATGGTGCGC TCGATCATTG CCCTGCACAA CCTGATCAAC 
AACAAGCTGG CCAACCGCGA CGCCGAGGAG GGCAAGAGCG ACAGCAAGGA GGCCAAGGAG 
AAGAATAAGG ATAGCAAGGA TAAGGACAAC AAGGAGACCA AGGACAAGGA CGGCAAGAAG 
GCGGAGGAGA AGGCCGACAA GGGCAAGGAC GAAGGCGGCA AGGGTTCGCG CAAATAGGAG 
CGGAAGAAAA AGCGCAAACC CCGTCGCAAA CGAAAGAAGC AGCGGGCGCA GGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Rpn8 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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