Slik cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slik
Entrez Gene ID	37893
Full Name	Sterile20-like kinase
Synonyms	CG4527,DPlkk1,Dmel\CG4527,PLKK1,Plkk/Slik,Plkk1,SLIK,SLK,Slik1,Slk,dPlkk,dPlkk/Slik,slik,slk
General protein information	Preferred Names Sterile20-like kinase Names CG4527 gene product from transcript CG4527-RB CG4527-PA CG4527-PB CG4527-PD CG4527-PE CG4527-PF CG4527-PG Slik-PA Slik-PB Slik-PD Slik-PE Slik-PF Slik-PG Ste20 kinase Sterile-20 dPlkk/Slik threonine kinase polo kinase kinase 1 sterile 20-like kinase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 60C7-60C8

mRNA and Protein(s)

mRNA	Protein	Name
NM_138064.4	NP_611908.2	Sterile20-like kinase, isoform A
NM_166669.4	NP_726441.1	Sterile20-like kinase, isoform B
NM_001014549.3	NP_001014549.1	Sterile20-like kinase, isoform E
NM_206214.4	NP_995936.2	Sterile20-like kinase, isoform G
NM_206213.3	NP_995935.1	Sterile20-like kinase, isoform D
NM_001144280.2	NP_001137752.1	Sterile20-like kinase, isoform F

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	STK10	XP_546239.2
Bos taurus (cattle)	STK10	NP_001179556.1
Rattus norvegicus (Norway rat)	Stk10	NP_062079.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010551	XP_314524.4
Homo sapiens (human)	STK10	NP_005981.3
	STK10	XP_002804682.1
Gallus gallus (chicken)	STK10	XP_414529.4
Caenorhabditis elegans (roundworm)	gck-4	NP_510290.1
Pan troglodytes (chimpanzee)	STK10	XP_518098.4
Mus musculus (house mouse)	Stk10	NP_033314.2
Danio rerio (zebrafish)	stk10	NP_957427.1
Drosophila melanogaster (fruit fly)	slik	NP_001014549.1
Xenopus tropicalis (tropical clawed frog)	stk10	NP_001072623.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The conserved C-terminal coiled-coil domain of Slik, which is necessary and sufficient for apical localization of the kinase in epithelial cells, is not required for Moesin phosphorylation but is critical for the growth-promoting function of Slik.
Title: Regulation of Catalytic and Non-catalytic Functions of the Drosophila Ste20 Kinase Slik by Activation Segment Phosphorylation.

Slik acts via distinct pathways to coordinate cell proliferation with epithelial cell behavior during tissue growth.
Title: Slik Sterile-20 kinase regulates Moesin activity to promote epithelial integrity during tissue growth.

Slik mediates growth and survival cues to promote cell proliferation and control cell survival during Drosophila development
Title: The Drosophila sterile-20 kinase slik controls cell proliferation and apoptosis during imaginal disc development.

The following Slik gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slik cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa11164	NM_138064.4 Latest version!	Drosophila melanogaster Sterile20-like kinase (Slik), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa11161	NM_206213.3 Latest version!	Drosophila melanogaster Sterile20-like kinase (Slik), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa11161	NM_166669.4 Latest version!	Drosophila melanogaster Sterile20-like kinase (Slik), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa11162	NM_206214.4 Latest version!	Drosophila melanogaster Sterile20-like kinase (Slik), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa11163	NM_001014549.3 Latest version!	Drosophila melanogaster Sterile20-like kinase (Slik), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa13241	NM_001144280.2 Latest version!	Drosophila melanogaster Sterile20-like kinase (Slik), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slik ( NM_138064.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611908.2 Drosophila melanogaster Sterile20-like kinase (Slik), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa11164
Clone ID Related Accession (Same CDS sequence)	NM_138064.4
Accession Version	NM_138064.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Sterile20-like kinase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_138064.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTTTA TCACCAACCT GAAGAAGGTC TTCCACCTCG GCGGGGGTGA GGCCAAGAAG 
AAGCGCCTGT ACAACAACAT CAAGATGGAC ACGGATCCGG CGGAGTTCTG GGAAATGGTC 
GGCGAACTGG GCGACGGAGC CTTTGGCAAG GTTTACAAGG CTCAGCACAA GGAGCAGAAG 
CGATTTGCTG CCGCAAAAAT GTGTCAGCTG GAGGACGAGG AGAACCTTAG CGATCACATG 
GTCGAGATCG ACATTCTGTC GGAGATCAAG CACCCAAATA TAGTGGAGCT GTATGAGGCC 
TTTTCCATCG ATGATAAGCT GTGGATGCTC ATCGAGTACT GCGACGGAGG GGCCCTGGAT 
AGCATTATGG TGGAGCTGGA AAAGCCGCTG ACTGAGCCGC AGATTGCCTA TGTTTGCAAG 
CACATGACCG AGGGTCTCAC CTTCCTGCAT CGCAACAAAG TCATCCATCG TGATTTGAAG 
GCAGGAAACG TGCTTCTCAC AATGGAAGGT GGCGTCAAGT TGGCGGACTT TGGCGTTTCG 
GCCAAAAACA AGCACACCAT GCAAAAGCAC GACACCTTCA TTGGCACTCC CTACTGGATG 
GCCCCGGAGC TGGTGCTTTG CGAAACTTTC CGGGACAACC CGTACGATCA CAAAGTGGAC 
ATCTGGTCAC TGGGCATCAC GCTCATTGAG CTGGCGCAGA TGGAGCCGCC CAACAGCGAG 
ATGTCGCCCA TGCGAGTGCT CTTGAAAATA CAAAAGAGCG AGCCGCCCAA GCTGGAGCAG 
CCTTCTCGAT GGAGCAAGGA GTTTAACGAC TTCCTGAAGA AGTCCTTAGT CAAAGATCCG 
CAGGTGAGGC CCACGACGGA TGTGCTGATG CAGCACGCTT TCATCAACAG AAATCTGGAT 
GCCAAGCCTA TCAAGGATCT GCTGCTTGAG TACAAGGCCG AGGTCGTGGA AGAGGTGGTG 
GATGATGAGA CCGAGGAACC CCGCAACTCG GCGCTCCAGC TCGACCTGGA CGATGACTCT 
GCTTCGCTGC AGAGCCAAGA CATTGACAAA CTTCCAGGTA CACCTACATC CATTTTGCGG 
GATGCCAAAG AGCAGTCCCA ACCAAGTAGC AGTTTACCAA TCGCAGCAGC GGCAACTGCA 
GCAGCAGCAG CTACAACCAC AACTAAAGCA ACCACTCCGG ACAGACCAAA CCACACAAAG 
GATGACAATG CTGAAGCGGC AGCCCAGCAG CCGCCTCATA CCAAAGTGCC TGCTCCAGCG 
CCGCCGTCGT CCCAACAAAC GCCACCACCG CAAGTTCAGC AGCCACCTAC ACCACCAGCC 
CAACCCACAG CGGCTGTGCT GCAAAAGAAA CCCGAAGATG TGGCAGCTGT TGCAGAAACA 
TCTGAGAAAA CCGAATCCGA CAAGAAGCAC TTTGTCAAAA AGGGTAAGGC CCCGCCCCCG 
CCGTCCCCAT TGGGTCTTGC AAATGCCAAG CCGGCTGCCA GCGACTCCCA GACGTCTCCC 
AAAAAACTGG CCACTCCCGA GCCCACTTCT CCAGTCACCA CGGCTATTGA GGTGGCCATT 
GGCCAAGAAG CGATGGAGCC CAAACCGCAG CCGCCATCGC CCACAGCCTC CTCCATTGTG 
TCTGTGCAAT CGGTGGCCTC TTCCAGCTCT TCGGGCAGTG TTTCAAATGC TGTTCTCAGC 
TCGAGCACTT CCCTTATTAC CATCAACAGC GATCCACCCA CACCGCATCA TCACCAACCG 
CTGCCACCTC AACCGCAGCA CTTGATTTTG CCAAACAGCT TGGAGTCGGT GAGTCAGATA 
ACAGTCGTGA CCAGCACCCA TCCGCCGGTA ATCATCGACA ACTCGGTGAT GCCACCGCAA 
AACGAGGTGA TCATCGTATC CAATGATATG AACAAGAGCA CTCACCTGCA TGAATCTTCG 
ACGGACGATG ATTTTCCATC GCTGGACGAC AGTTTGGGTG ATGCCACCAC GCCGCCCCAC 
AAGCAATCCT CAATGATATT GGCTGTTAAT GAGCCAGCAG GTGTGGTTCC TGCTCCTCCG 
TCGCAACCGC AAACGTCTTC CACTGTTCAC GCACGCAAAC TGGATGAAAG TGAGGTGTTA 
ATCGTCAGTC CGTCCTACGC GGATGATGAC TCGGCCTATA ATACGGCATC TGGCAGTCAT 
AGCCACGATC ACAGTGACCA TCTGATGGAC ACGAGCCACG TCTCCGTGGT CACCGTGGGC 
GATGAGGGAG TTAAGGTGAA GGACAGCAGC AATGAGCTGG TCAAGCGTCA GCCCAATGGA 
GTTGGCATTG TGCCGGAAGA CGTAAACATA ATTGTAAACC GATTCAAACA GGAGAAGCGG 
TCGCCGGACA GCTCGCTGAG CGAGAATGGC TCGGTGCGCG GACGGCGTGG TATTGAGGTG 
CTGGTTGGCG GCAGCGGAGG ATCCGACGTG GACAGCATTG GCACAAACAC TAGTCAGGAA 
AGCCGGCACG AAACGGATCA CAACAACAAG CAGCAGTATC CAGCGGCCGC GCTGATGCCT 
CCACCACCTC CCTCGTTGAC GAATAACCAC AATCACGAGA CCATTGATGA GGAAGAGGAG 
GTGGTGATCC GACCCAAGGC TAGGGTTCCG GCCGTAGTTA AGTCTGCAAA TGCTCAAGGT 
CTTACCAAGG AAGAGATTGA GCTGCGCAAT CTGCGCAAGA AGACACGCAA GCGCACCCGC 
AAATTCGAGA TCGATGGCGT GCAAATGACC ACCACCACAA GCCGAGTGAT CTACGGGGAC 
GATGAGAACG GACGAATCTA CGATGATCAC GACTTCCGTA AGCAGGAGCT ACGTGAATTA 
AAGCTGCTGC AGAAGCAGGA GAAGAAACAG CAGACGGAAC TGCACTTAAA GGAGCAGCAG 
GCCAAGGAAC AGCAGGATCG TCGCTTTGAG CAGGAGCGCT CCTCGCTGGA AAAGACCTAC 
GAAGCTGACA TGGATATGTT GGCGCGACAG CACAAGCAGC TGGTGGAAAA GACAGAGCAG 
ACGCAGGAGA ACGAGCTGCG CTCCTCATCT AAACGCATTC GCTCCGAGCA GGAGCAAGAG 
TTGAAGATCT TCCGAGAGAA TCTCAAGCAG GAGATCCGAC TGCTCAAGCA GGAAGTTGAC 
CTGTTTCCCA AGGACAAGCG CAAGGATGAG TTCAAGCAGC GGCGCTCAGC CATGGAACTA 
GACCACGAAG AGAAGGAACG CGCCTTCCTG GATTCTTTAA AGGAGAGGCA TGAGCTTCTA 
CTAAGGCGAT TAAGCGAGAA GCATCGGGAC CACCTGGCCA CCATCAACCG CAACTTCTTG 
CAGCAGAAAC AGAACGCCAT GAGGACGCGG GAGGCACTTC TCTGGGAACT AGAGGAGAAG 
CAACTTCACG AACGACATCA GTTGTCCAAG CGGCATGTAA AGGAACTATG CTTCATGCAG 
CGACATCAGA TGATAATCCG GCACGAGAAG GAGCTGGACC AGGTGAAGCG TATGTTGCAG 
CGTAAGGAGG AGGACATGGT CAAGAAACAG ACGATGGAAA AGCGGGCGCT GCCAAAGCGC 
ATCCGTGCGG AACGCAAGGC GCGAGATCTT ATGTTCCGTG AATCCCTTCG AATTTCCACG 
AACCTAGATC CTGAAATCGA ACGCGATCGC TTGAAGAAGT TCCAGGAGCA AGAGAAAAAG 
CGTTATATGC AGGAGGAGCG ACGCTTCGAA GTCAAGCATC AAAAGCAATT GGAAGAGCTA 
CGCGCTACAC GCGAAAGCGC CATAAAAGAG CTGGAGCAAC TTCAGAACGA AAAGCGAAGG 
GCTCTGGTGG AGCACGAACA CTCCAAGCTT TCCGAGATCG ATGAGCGACT GAAGGGGGAG 
CTGCGCGAGT GGCGCGAACA GTTGGTGCCC CGTAAGCAGG TGTGTATGCA AATCCCCGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611908.2
CDS	383..4285
Translation	MSFITNLKKVFHLGGGEAKKKRLYNNIKMDTDPAEFWEMVGELGDGAFGKVYKAQHKEQKRFAAAKMCQLEDEENLSDHMVEIDILSEIKHPNIVELYEAFSIDDKLWMLIEYCDGGALDSIMVELEKPLTEPQIAYVCKHMTEGLTFLHRNKVIHRDLKAGNVLLTMEGGVKLADFGVSAKNKHTMQKHDTFIGTPYWMAPELVLCETFRDNPYDHKVDIWSLGITLIELAQMEPPNSEMSPMRVLLKIQKSEPPKLEQPSRWSKEFNDFLKKSLVKDPQVRPTTDVLMQHAFINRNLDAKPIKDLLLEYKAEVVEEVVDDETEEPRNSALQLDLDDDSASLQSQDIDKLPGTPTSILRDAKEQSQPSSSLPIAAAATAAAAATTTTKATTPDRPNHTKDDNAEAAAQQPPHTKVPAPAPPSSQQTPPPQVQQPPTPPAQPTAAVLQKKPEDVAAVAETSEKTESDKKHFVKKGKAPPPPSPLGLANAKPAASDSQTSPKKLATPEPTSPVTTAIEVAIGQEAMEPKPQPPSPTASSIVSVQSVASSSSSGSVSNAVLSSSTSLITINSDPPTPHHHQPLPPQPQHLILPNSLESVSQITVVTSTHPPVIIDNSVMPPQNEVIIVSNDMNKSTHLHESSTDDDFPSLDDSLGDATTPPHKQSSMILAVNEPAGVVPAPPSQPQTSSTVHARKLDESEVLIVSPSYADDDSAYNTASGSHSHDHSDHLMDTSHVSVVTVGDEGVKVKDSSNELVKRQPNGVGIVPEDVNIIVNRFKQEKRSPDSSLSENGSVRGRRGIEVLVGGSGGSDVDSIGTNTSQESRHETDHNNKQQYPAAALMPPPPPSLTNNHNHETIDEEEEVVIRPKARVPAVVKSANAQGLTKEEIELRNLRKKTRKRTRKFEIDGVQMTTTTSRVIYGDDENGRIYDDHDFRKQELRELKLLQKQEKKQQTELHLKEQQAKEQQDRRFEQERSSLEKTYEADMDMLARQHKQLVEKTEQTQENELRSSSKRIRSEQEQELKIFRENLKQEIRLLKQEVDLFPKDKRKDEFKQRRSAMELDHEEKERAFLDSLKERHELLLRRLSEKHRDHLATINRNFLQQKQNAMRTREALLWELEEKQLHERHQLSKRHVKELCFMQRHQMIIRHEKELDQVKRMLQRKEEDMVKKQTMEKRALPKRIRAERKARDLMFRESLRISTNLDPEIERDRLKKFQEQEKKRYMQEERRFEVKHQKQLEELRATRESAIKELEQLQNEKRRALVEHEHSKLSEIDERLKGELREWREQLVPRKQVCMQIPV

Target ORF information:

RefSeq Version	NM_138064.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Sterile20-like kinase (Slik), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138064.4

ORF Insert Sequence:

ATGTCGTTTA TCACCAACCT GAAGAAGGTC TTCCACCTCG GCGGGGGTGA GGCCAAGAAG 
AAGCGCCTGT ACAACAACAT CAAGATGGAC ACGGATCCGG CGGAGTTCTG GGAAATGGTC 
GGCGAACTGG GCGACGGAGC CTTTGGCAAG GTTTACAAGG CTCAGCACAA GGAGCAGAAG 
CGATTTGCTG CCGCAAAAAT GTGTCAGCTG GAGGACGAGG AGAACCTTAG CGATCACATG 
GTCGAGATCG ACATTCTGTC GGAGATCAAG CACCCAAATA TAGTGGAGCT GTATGAGGCC 
TTTTCCATCG ATGATAAGCT GTGGATGCTC ATCGAGTACT GCGACGGAGG GGCCCTGGAT 
AGCATTATGG TGGAGCTGGA AAAGCCGCTG ACTGAGCCGC AGATTGCCTA TGTTTGCAAG 
CACATGACCG AGGGTCTCAC CTTCCTGCAT CGCAACAAAG TCATCCATCG TGATTTGAAG 
GCAGGAAACG TGCTTCTCAC AATGGAAGGT GGCGTCAAGT TGGCGGACTT TGGCGTTTCG 
GCCAAAAACA AGCACACCAT GCAAAAGCAC GACACCTTCA TTGGCACTCC CTACTGGATG 
GCCCCGGAGC TGGTGCTTTG CGAAACTTTC CGGGACAACC CGTACGATCA CAAAGTGGAC 
ATCTGGTCAC TGGGCATCAC GCTCATTGAG CTGGCGCAGA TGGAGCCGCC CAACAGCGAG 
ATGTCGCCCA TGCGAGTGCT CTTGAAAATA CAAAAGAGCG AGCCGCCCAA GCTGGAGCAG 
CCTTCTCGAT GGAGCAAGGA GTTTAACGAC TTCCTGAAGA AGTCCTTAGT CAAAGATCCG 
CAGGTGAGGC CCACGACGGA TGTGCTGATG CAGCACGCTT TCATCAACAG AAATCTGGAT 
GCCAAGCCTA TCAAGGATCT GCTGCTTGAG TACAAGGCCG AGGTCGTGGA AGAGGTGGTG 
GATGATGAGA CCGAGGAACC CCGCAACTCG GCGCTCCAGC TCGACCTGGA CGATGACTCT 
GCTTCGCTGC AGAGCCAAGA CATTGACAAA CTTCCAGGTA CACCTACATC CATTTTGCGG 
GATGCCAAAG AGCAGTCCCA ACCAAGTAGC AGTTTACCAA TCGCAGCAGC GGCAACTGCA 
GCAGCAGCAG CTACAACCAC AACTAAAGCA ACCACTCCGG ACAGACCAAA CCACACAAAG 
GATGACAATG CTGAAGCGGC AGCCCAGCAG CCGCCTCATA CCAAAGTGCC TGCTCCAGCG 
CCGCCGTCGT CCCAACAAAC GCCACCACCG CAAGTTCAGC AGCCACCTAC ACCACCAGCC 
CAACCCACAG CGGCTGTGCT GCAAAAGAAA CCCGAAGATG TGGCAGCTGT TGCAGAAACA 
TCTGAGAAAA CCGAATCCGA CAAGAAGCAC TTTGTCAAAA AGGGTAAGGC CCCGCCCCCG 
CCGTCCCCAT TGGGTCTTGC AAATGCCAAG CCGGCTGCCA GCGACTCCCA GACGTCTCCC 
AAAAAACTGG CCACTCCCGA GCCCACTTCT CCAGTCACCA CGGCTATTGA GGTGGCCATT 
GGCCAAGAAG CGATGGAGCC CAAACCGCAG CCGCCATCGC CCACAGCCTC CTCCATTGTG 
TCTGTGCAAT CGGTGGCCTC TTCCAGCTCT TCGGGCAGTG TTTCAAATGC TGTTCTCAGC 
TCGAGCACTT CCCTTATTAC CATCAACAGC GATCCACCCA CACCGCATCA TCACCAACCG 
CTGCCACCTC AACCGCAGCA CTTGATTTTG CCAAACAGCT TGGAGTCGGT GAGTCAGATA 
ACAGTCGTGA CCAGCACCCA TCCGCCGGTA ATCATCGACA ACTCGGTGAT GCCACCGCAA 
AACGAGGTGA TCATCGTATC CAATGATATG AACAAGAGCA CTCACCTGCA TGAATCTTCG 
ACGGACGATG ATTTTCCATC GCTGGACGAC AGTTTGGGTG ATGCCACCAC GCCGCCCCAC 
AAGCAATCCT CAATGATATT GGCTGTTAAT GAGCCAGCAG GTGTGGTTCC TGCTCCTCCG 
TCGCAACCGC AAACGTCTTC CACTGTTCAC GCACGCAAAC TGGATGAAAG TGAGGTGTTA 
ATCGTCAGTC CGTCCTACGC GGATGATGAC TCGGCCTATA ATACGGCATC TGGCAGTCAT 
AGCCACGATC ACAGTGACCA TCTGATGGAC ACGAGCCACG TCTCCGTGGT CACCGTGGGC 
GATGAGGGAG TTAAGGTGAA GGACAGCAGC AATGAGCTGG TCAAGCGTCA GCCCAATGGA 
GTTGGCATTG TGCCGGAAGA CGTAAACATA ATTGTAAACC GATTCAAACA GGAGAAGCGG 
TCGCCGGACA GCTCGCTGAG CGAGAATGGC TCGGTGCGCG GACGGCGTGG TATTGAGGTG 
CTGGTTGGCG GCAGCGGAGG ATCCGACGTG GACAGCATTG GCACAAACAC TAGTCAGGAA 
AGCCGGCACG AAACGGATCA CAACAACAAG CAGCAGTATC CAGCGGCCGC GCTGATGCCT 
CCACCACCTC CCTCGTTGAC GAATAACCAC AATCACGAGA CCATTGATGA GGAAGAGGAG 
GTGGTGATCC GACCCAAGGC TAGGGTTCCG GCCGTAGTTA AGTCTGCAAA TGCTCAAGGT 
CTTACCAAGG AAGAGATTGA GCTGCGCAAT CTGCGCAAGA AGACACGCAA GCGCACCCGC 
AAATTCGAGA TCGATGGCGT GCAAATGACC ACCACCACAA GCCGAGTGAT CTACGGGGAC 
GATGAGAACG GACGAATCTA CGATGATCAC GACTTCCGTA AGCAGGAGCT ACGTGAATTA 
AAGCTGCTGC AGAAGCAGGA GAAGAAACAG CAGACGGAAC TGCACTTAAA GGAGCAGCAG 
GCCAAGGAAC AGCAGGATCG TCGCTTTGAG CAGGAGCGCT CCTCGCTGGA AAAGACCTAC 
GAAGCTGACA TGGATATGTT GGCGCGACAG CACAAGCAGC TGGTGGAAAA GACAGAGCAG 
ACGCAGGAGA ACGAGCTGCG CTCCTCATCT AAACGCATTC GCTCCGAGCA GGAGCAAGAG 
TTGAAGATCT TCCGAGAGAA TCTCAAGCAG GAGATCCGAC TGCTCAAGCA GGAAGTTGAC 
CTGTTTCCCA AGGACAAGCG CAAGGATGAG TTCAAGCAGC GGCGCTCAGC CATGGAACTA 
GACCACGAAG AGAAGGAACG CGCCTTCCTG GATTCTTTAA AGGAGAGGCA TGAGCTTCTA 
CTAAGGCGAT TAAGCGAGAA GCATCGGGAC CACCTGGCCA CCATCAACCG CAACTTCTTG 
CAGCAGAAAC AGAACGCCAT GAGGACGCGG GAGGCACTTC TCTGGGAACT AGAGGAGAAG 
CAACTTCACG AACGACATCA GTTGTCCAAG CGGCATGTAA AGGAACTATG CTTCATGCAG 
CGACATCAGA TGATAATCCG GCACGAGAAG GAGCTGGACC AGGTGAAGCG TATGTTGCAG 
CGTAAGGAGG AGGACATGGT CAAGAAACAG ACGATGGAAA AGCGGGCGCT GCCAAAGCGC 
ATCCGTGCGG AACGCAAGGC GCGAGATCTT ATGTTCCGTG AATCCCTTCG AATTTCCACG 
AACCTAGATC CTGAAATCGA ACGCGATCGC TTGAAGAAGT TCCAGGAGCA AGAGAAAAAG 
CGTTATATGC AGGAGGAGCG ACGCTTCGAA GTCAAGCATC AAAAGCAATT GGAAGAGCTA 
CGCGCTACAC GCGAAAGCGC CATAAAAGAG CTGGAGCAAC TTCAGAACGA AAAGCGAAGG 
GCTCTGGTGG AGCACGAACA CTCCAAGCTT TCCGAGATCG ATGAGCGACT GAAGGGGGAG 
CTGCGCGAGT GGCGCGAACA GTTGGTGCCC CGTAAGCAGG TGTGTATGCA AATCCCCGTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slik ( NM_166669.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726441.1 Drosophila melanogaster Sterile20-like kinase (Slik), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa11161
Clone ID Related Accession (Same CDS sequence)	NM_166669.4 , NM_206213.3
Accession Version	NM_166669.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Sterile20-like kinase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166669.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTTTA TCACCAACCT GAAGAAGGTC TTCCACCTCG GCGGGGGTGA GGCCAAGAAG 
AAGCGCCTGT ACAACAACAT CAAGATGGAC ACGGATCCGG CGGAGTTCTG GGAAATGGTC 
GGCGAACTGG GCGACGGAGC CTTTGGCAAG GTTTACAAGG CTCAGCACAA GGAGCAGAAG 
CGATTTGCTG CCGCAAAAAT GTGTCAGCTG GAGGACGAGG AGAACCTTAG CGATCACATG 
GTCGAGATCG ACATTCTGTC GGAGATCAAG CACCCAAATA TAGTGGAGCT GTATGAGGCC 
TTTTCCATCG ATGATAAGCT GTGGATGCTC ATCGAGTACT GCGACGGAGG GGCCCTGGAT 
AGCATTATGG TGGAGCTGGA AAAGCCGCTG ACTGAGCCGC AGATTGCCTA TGTTTGCAAG 
CACATGACCG AGGGTCTCAC CTTCCTGCAT CGCAACAAAG TCATCCATCG TGATTTGAAG 
GCAGGAAACG TGCTTCTCAC AATGGAAGGT GGCGTCAAGT TGGCGGACTT TGGCGTTTCG 
GCCAAAAACA AGCACACCAT GCAAAAGCAC GACACCTTCA TTGGCACTCC CTACTGGATG 
GCCCCGGAGC TGGTGCTTTG CGAAACTTTC CGGGACAACC CGTACGATCA CAAAGTGGAC 
ATCTGGTCAC TGGGCATCAC GCTCATTGAG CTGGCGCAGA TGGAGCCGCC CAACAGCGAG 
ATGTCGCCCA TGCGAGTGCT CTTGAAAATA CAAAAGAGCG AGCCGCCCAA GCTGGAGCAG 
CCTTCTCGAT GGAGCAAGGA GTTTAACGAC TTCCTGAAGA AGTCCTTAGT CAAAGATCCG 
CAGGTGAGGC CCACGACGGA TGTGCTGATG CAGCACGCTT TCATCAACAG AAATCTGGAT 
GCCAAGCCTA TCAAGGATCT GCTGCTTGAG TACAAGGCCG AGGTCGTGGA AGAGGTGGTG 
GATGATGAGA CCGAGGAACC CCGCAACTCG GCGCTCCAGC TCGACCTGGA CGATGACTCT 
GCTTCGCTGC AGAGCCAAGA CATTGACAAA CTTCCAGGTA CACCTACATC CATTTTGCGG 
GATGCCAAAG AGCAGTCCCA ACCAAGTAGC AGTTTACCAA TCGCAGCAGC GGCAACTGCA 
GCAGCAGCAG CTACAACCAC AACTAAAGCA ACCACTCCGG ACAGACCAAA CCACACAAAG 
GATGACAATG CTGAAGCGGC AGCCCAGCAG CCGCCTCATA CCAAAGTGCC TGCTCCAGCG 
CCGCCGTCGT CCCAACAAAC GCCACCACCG CAAGTTCAGC AGCCACCTAC ACCACCAGCC 
CAACCCACAG CGGCTGTGCT GCAAAAGAAA CCCGAAGATG TGGCAGCTGT TGCAGAAACA 
TCTGAGAAAA CCGAATCCGA CAAGAAGCAC TTTGTCAAAA AGGGTAAGGC CCCGCCCCCG 
CCGTCCCCAT TGGGTCTTGC AAATGCCAAG CCGGCTGCCA GCGACTCCCA GACGTCTCCC 
AAAAAACTGG CCACTCCCGA GCCCACTTCT CCAGTCACCA CGGCTATTGA GGTGGCCATT 
GGCCAAGAAG CGATGGAGCC CAAACCGCAG CCGCCATCGC CCACAGCCTC CTCCATTGTG 
TCTGTGCAAT CGGTGGCCTC TTCCAGCTCT TCGGGCAGTG TTTCAAATGC TGTTCTCAGC 
TCGAGCACTT CCCTTATTAC CATCAACAGC GATCCACCCA CACCGCATCA TCACCAACCG 
CTGCCACCTC AACCGCAGCA CTTGATTTTG CCAAACAGCT TGGAGTCGGT GAGTCAGATA 
ACAGTCGTGA CCAGCACCCA TCCGCCGGTA ATCATCGACA ACTCGGTGAT GCCACCGCAA 
AACGAGGTGA TCATCGTATC CAATGATATG AACAAGAGCA CTCACCTGCA TGAATCTTCG 
ACGGACGATG ATTTTCCATC GCTGGACGAC AGTTTGGGTG ATGCCACCAC GCCGCCCCAC 
AAGCAATCCT CAATGATATT GGCTGTTAAT GAGCCAGCAG GTGTGGTTCC TGCTCCTCCG 
TCGCAACCGC AAACGTCTTC CACTGTTCAC GCACGCAAAC TGGATGAAAG TGAGGTGTTA 
ATCGTCAGTC CGTCCTACGC GGATGATGAC TCGGCCTATA ATACGGCATC TGGCAGTCAT 
AGCCACGATC ACAGTGACCA TCTGATGGAC ACGAGCCACG TCTCCGTGGT CACCGTGGGC 
GATGAGGGAG TTAAGGTGAA GGACAGCAGC AATGAGCTGG TCAAGCGTCA GCCCAATGGA 
GTTGGCATTG TGCCGGAAGA CGTAAACATA ATTGTAAACC GATTCAAACA GGAGAAGCGG 
TCGCCGGACA GCTCGCTGAG CGAGAATGGC TCGGTGCGCG GACGGCGTGG TATTGAGGTG 
CTGGTTGGCG GCAGCGGAGG ATCCGACGTG GACAGCATTG GCACAAACAC TAGTCAGGAA 
AGCCGGCACG AAACGGATCA CAACAACAAG CAGCAGTATC CAGCGGCCGC GCTGATGCCT 
CCACCACCTC CCTCGTTGAC GAATAACCAC AATCACGAGA CCATTGATGA GGAAGAGGAG 
GTGGTGATCC GACCCAAGGC TAGGGTTCCG GCCGTAGTTA AGTCTGCAAA TGCTCAAGGT 
CTTACCAAGG AAGAGATTGA GCTGCGCAAT CTGCGCAAGA AGACACGCAA GCGCACCCGC 
AAATTCGAGA TCGATGGCGT GCAAATGACC ACCACCACAA GCCGAGTGAT CTACGGGGAC 
GATGAGAACG GACGAATCTA CGATGATCAC GACTTCCGTA AGCAGGAGCT ACGTGAATTA 
AAGCTGCTGC AGAAGCAGGA GAAGAAACAG CAGACGGAAC TGCACTTAAA GGAGCAGCAG 
GCCAAGGAAC AGCAGGATCG TCGCTTTGAG CAGGAGCGCT CCTCGCTGGA AAAGACCTAC 
GAAGCTGACA TGGATATGTT GGCGCGACAG CACAAGCAGC TGGTGGAAAA GACAGAGCAG 
ACGCAGGAGA ACGAGCTGCG CTCCTCATCT AAACGCATTC GCTCCGAGCA GGAGCAAGAG 
TTGAAGATCT TCCGAGAGAA TCTCAAGCAG GAGATCCGAC TGCTCAAGCA GGAAGTTGAC 
CTGTTTCCCA AGGACAAGCG CAAGGATGAG TTCAAGCAGC GGCGCTCAGC CATGGAACTA 
GACCACGAAG AGAAGGAACG CGCCTTCCTG GATTCTTTAA AGGAGAGGCA TGAGCTTCTA 
CTAAGGCGAT TAAGCGAGAA GCATCGGGAC CACCTGGCCA CCATCAACCG CAACTTCTTG 
CAGCAGAAAC AGAACGCCAT GAGGACGCGG GAGGCACTTC TCTGGGAACT AGAGGAGAAG 
CAACTTCACG AACGACATCA GTTGTCCAAG CGGCATGTAA AGGAACTATG CTTCATGCAG 
CGACATCAGA TGATAATCCG GCACGAGAAG GAGCTGGACC AGGTGAAGCG TATGTTGCAG 
CGTAAGGAGG AGGACATGGT CAAGAAACAG ACGATGGAAA AGCGGGCGCT GCCAAAGCGC 
ATCCGTGCGG AACGCAAGGC GCGAGATCTT ATGTTCCGTG AATCCCTTCG AATTTCCACG 
AACCTAGATC CTGAAATCGA ACGCGATCGC TTGAAGAAGT TCCAGGAGCA AGAGAAAAAG 
CGTTATATGC AGGAGGAGCG ACGCTTCGAA GTCAAGCATC AAAAGCAATT GGAAGAGCTA 
CGCGCTACAC GCGAAAGCGC CATAAAAGAG CTGGAGCAAC TTCAGAACGA AAAGCGAAGG 
GCTCTGGTGG AGCACGAACA CTCCAAGCTT TCCGAGATCG ATGAGCGACT GAAGGGGGAG 
CTGCGCGAGT GGCGCGAACA GTTGGTGCCC CGTAAGCAGG AGCTGAATCG ACAGATCAAA 
CTGGCCATTG ATCAGCACGA AAAGCGTTTT GGTCTTGTGA CCAATCGCGA GGAGTTCGAG 
GATCAGGAGG TCAAGCTGCC CGCCCACTTG CGAAACATTT ACAACGAGCG ATCCTCACGC 
ATTCTGCCCC GCAACACCTT TATCGACCCT CAAAACATGC CGCGCTCTCG CTCCTCGCTC 
CTAATGATGG GGGGTGGTGG CAACCGACTG TCCAACTTCC GCGGATCAGC TCCAGATCTT 
AGCCGCAGTG TGCCCAACAC TCCTATTATG CACAAACAAC AGAGATCGCA GATGATAGGC 
GATCAGGTGC TAGAAGAGGA CGAGGAGCAG ATGCTGAGCG AGCAGATGAA GCGGGCCAGC 
GTCGCCACGC TTCCGGCGCA AAACCAACTT CGCCTTATTA CTCAGCTTCC GGCAAACGAG 
CTCGTGAAGG TAGTGACCTC CACTCCACCA CTTAACAGCG CGGAGGATGT GAACTCCAAG 
CCATCCATGA GCACATTCAG AGGCAGTACG CCGCCCAAAA CACCAGATGA TCTTGGCTTG 
GTCACCAATC GATTCAGCCG CCTGGACACC AAAGCTGCAG CGGAGGCCAG CGGCGTGGAG 
TTGCGCATTC ACGAGGGACC AGTGCTAACC GCTCACACTC AGCGGCTGCT GCACACCACC 
TCCTTTGCTA TCAAGCGACC CTCACTGGCC AGCCGGAGCA GCGGCAGATC CTCCCTGAGC 
AGTGCGCAGT CCATGTACAA CATAAATGAG CTGTCCACGC GGTTCACCAG TGATGCGGAC 
CTGATCTTTG ACTCCGGACG CAAGGCTGCC GCCCTGCTAG ACGAAGTGCA ACCGCAGCTA 
CGGTCCTCCG TTAAGCCAGG ACATAGTCGC GCCAATGGAA GCGTCGTCTC AGCCGACTCG 
GCCGCCTCTT CCGAGGATAT CTACTACGAC CTGTATGCGG CGCAGTACAA ACTGCCGAGA 
CAACTACAGC AACGGCTGCA ACAAGAGGTT AGTCCTTTGA TCTATCCCCA GTGTGCTTCC 
CAGCCATTCG ATACGACCCA GGGCCCTGAA ACTGGAGCTC GCCGCCTTGA GGAAACCTTC 
GCCCAACAGC TGGACGAGAT GGAGACCCTG TACGGCGGCG CCCTGATTGT GTCCATGCCC 
TCGGACACGC TGCAGCGAGA TCACTTCACC GGCTCGACGC GCAGCAGCCT CAGCTCGTAT 
TCCGAGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726441.1
CDS	383..5494
Translation	MSFITNLKKVFHLGGGEAKKKRLYNNIKMDTDPAEFWEMVGELGDGAFGKVYKAQHKEQKRFAAAKMCQLEDEENLSDHMVEIDILSEIKHPNIVELYEAFSIDDKLWMLIEYCDGGALDSIMVELEKPLTEPQIAYVCKHMTEGLTFLHRNKVIHRDLKAGNVLLTMEGGVKLADFGVSAKNKHTMQKHDTFIGTPYWMAPELVLCETFRDNPYDHKVDIWSLGITLIELAQMEPPNSEMSPMRVLLKIQKSEPPKLEQPSRWSKEFNDFLKKSLVKDPQVRPTTDVLMQHAFINRNLDAKPIKDLLLEYKAEVVEEVVDDETEEPRNSALQLDLDDDSASLQSQDIDKLPGTPTSILRDAKEQSQPSSSLPIAAAATAAAAATTTTKATTPDRPNHTKDDNAEAAAQQPPHTKVPAPAPPSSQQTPPPQVQQPPTPPAQPTAAVLQKKPEDVAAVAETSEKTESDKKHFVKKGKAPPPPSPLGLANAKPAASDSQTSPKKLATPEPTSPVTTAIEVAIGQEAMEPKPQPPSPTASSIVSVQSVASSSSSGSVSNAVLSSSTSLITINSDPPTPHHHQPLPPQPQHLILPNSLESVSQITVVTSTHPPVIIDNSVMPPQNEVIIVSNDMNKSTHLHESSTDDDFPSLDDSLGDATTPPHKQSSMILAVNEPAGVVPAPPSQPQTSSTVHARKLDESEVLIVSPSYADDDSAYNTASGSHSHDHSDHLMDTSHVSVVTVGDEGVKVKDSSNELVKRQPNGVGIVPEDVNIIVNRFKQEKRSPDSSLSENGSVRGRRGIEVLVGGSGGSDVDSIGTNTSQESRHETDHNNKQQYPAAALMPPPPPSLTNNHNHETIDEEEEVVIRPKARVPAVVKSANAQGLTKEEIELRNLRKKTRKRTRKFEIDGVQMTTTTSRVIYGDDENGRIYDDHDFRKQELRELKLLQKQEKKQQTELHLKEQQAKEQQDRRFEQERSSLEKTYEADMDMLARQHKQLVEKTEQTQENELRSSSKRIRSEQEQELKIFRENLKQEIRLLKQEVDLFPKDKRKDEFKQRRSAMELDHEEKERAFLDSLKERHELLLRRLSEKHRDHLATINRNFLQQKQNAMRTREALLWELEEKQLHERHQLSKRHVKELCFMQRHQMIIRHEKELDQVKRMLQRKEEDMVKKQTMEKRALPKRIRAERKARDLMFRESLRISTNLDPEIERDRLKKFQEQEKKRYMQEERRFEVKHQKQLEELRATRESAIKELEQLQNEKRRALVEHEHSKLSEIDERLKGELREWREQLVPRKQELNRQIKLAIDQHEKRFGLVTNREEFEDQEVKLPAHLRNIYNERSSRILPRNTFIDPQNMPRSRSSLLMMGGGGNRLSNFRGSAPDLSRSVPNTPIMHKQQRSQMIGDQVLEEDEEQMLSEQMKRASVATLPAQNQLRLITQLPANELVKVVTSTPPLNSAEDVNSKPSMSTFRGSTPPKTPDDLGLVTNRFSRLDTKAAAEASGVELRIHEGPVLTAHTQRLLHTTSFAIKRPSLASRSSGRSSLSSAQSMYNINELSTRFTSDADLIFDSGRKAAALLDEVQPQLRSSVKPGHSRANGSVVSADSAASSEDIYYDLYAAQYKLPRQLQQRLQQEVSPLIYPQCASQPFDTTQGPETGARRLEETFAQQLDEMETLYGGALIVSMPSDTLQRDHFTGSTRSSLSSYSEG

Target ORF information:

RefSeq Version	NM_166669.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Sterile20-like kinase (Slik), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166669.4

ORF Insert Sequence:

ATGTCGTTTA TCACCAACCT GAAGAAGGTC TTCCACCTCG GCGGGGGTGA GGCCAAGAAG 
AAGCGCCTGT ACAACAACAT CAAGATGGAC ACGGATCCGG CGGAGTTCTG GGAAATGGTC 
GGCGAACTGG GCGACGGAGC CTTTGGCAAG GTTTACAAGG CTCAGCACAA GGAGCAGAAG 
CGATTTGCTG CCGCAAAAAT GTGTCAGCTG GAGGACGAGG AGAACCTTAG CGATCACATG 
GTCGAGATCG ACATTCTGTC GGAGATCAAG CACCCAAATA TAGTGGAGCT GTATGAGGCC 
TTTTCCATCG ATGATAAGCT GTGGATGCTC ATCGAGTACT GCGACGGAGG GGCCCTGGAT 
AGCATTATGG TGGAGCTGGA AAAGCCGCTG ACTGAGCCGC AGATTGCCTA TGTTTGCAAG 
CACATGACCG AGGGTCTCAC CTTCCTGCAT CGCAACAAAG TCATCCATCG TGATTTGAAG 
GCAGGAAACG TGCTTCTCAC AATGGAAGGT GGCGTCAAGT TGGCGGACTT TGGCGTTTCG 
GCCAAAAACA AGCACACCAT GCAAAAGCAC GACACCTTCA TTGGCACTCC CTACTGGATG 
GCCCCGGAGC TGGTGCTTTG CGAAACTTTC CGGGACAACC CGTACGATCA CAAAGTGGAC 
ATCTGGTCAC TGGGCATCAC GCTCATTGAG CTGGCGCAGA TGGAGCCGCC CAACAGCGAG 
ATGTCGCCCA TGCGAGTGCT CTTGAAAATA CAAAAGAGCG AGCCGCCCAA GCTGGAGCAG 
CCTTCTCGAT GGAGCAAGGA GTTTAACGAC TTCCTGAAGA AGTCCTTAGT CAAAGATCCG 
CAGGTGAGGC CCACGACGGA TGTGCTGATG CAGCACGCTT TCATCAACAG AAATCTGGAT 
GCCAAGCCTA TCAAGGATCT GCTGCTTGAG TACAAGGCCG AGGTCGTGGA AGAGGTGGTG 
GATGATGAGA CCGAGGAACC CCGCAACTCG GCGCTCCAGC TCGACCTGGA CGATGACTCT 
GCTTCGCTGC AGAGCCAAGA CATTGACAAA CTTCCAGGTA CACCTACATC CATTTTGCGG 
GATGCCAAAG AGCAGTCCCA ACCAAGTAGC AGTTTACCAA TCGCAGCAGC GGCAACTGCA 
GCAGCAGCAG CTACAACCAC AACTAAAGCA ACCACTCCGG ACAGACCAAA CCACACAAAG 
GATGACAATG CTGAAGCGGC AGCCCAGCAG CCGCCTCATA CCAAAGTGCC TGCTCCAGCG 
CCGCCGTCGT CCCAACAAAC GCCACCACCG CAAGTTCAGC AGCCACCTAC ACCACCAGCC 
CAACCCACAG CGGCTGTGCT GCAAAAGAAA CCCGAAGATG TGGCAGCTGT TGCAGAAACA 
TCTGAGAAAA CCGAATCCGA CAAGAAGCAC TTTGTCAAAA AGGGTAAGGC CCCGCCCCCG 
CCGTCCCCAT TGGGTCTTGC AAATGCCAAG CCGGCTGCCA GCGACTCCCA GACGTCTCCC 
AAAAAACTGG CCACTCCCGA GCCCACTTCT CCAGTCACCA CGGCTATTGA GGTGGCCATT 
GGCCAAGAAG CGATGGAGCC CAAACCGCAG CCGCCATCGC CCACAGCCTC CTCCATTGTG 
TCTGTGCAAT CGGTGGCCTC TTCCAGCTCT TCGGGCAGTG TTTCAAATGC TGTTCTCAGC 
TCGAGCACTT CCCTTATTAC CATCAACAGC GATCCACCCA CACCGCATCA TCACCAACCG 
CTGCCACCTC AACCGCAGCA CTTGATTTTG CCAAACAGCT TGGAGTCGGT GAGTCAGATA 
ACAGTCGTGA CCAGCACCCA TCCGCCGGTA ATCATCGACA ACTCGGTGAT GCCACCGCAA 
AACGAGGTGA TCATCGTATC CAATGATATG AACAAGAGCA CTCACCTGCA TGAATCTTCG 
ACGGACGATG ATTTTCCATC GCTGGACGAC AGTTTGGGTG ATGCCACCAC GCCGCCCCAC 
AAGCAATCCT CAATGATATT GGCTGTTAAT GAGCCAGCAG GTGTGGTTCC TGCTCCTCCG 
TCGCAACCGC AAACGTCTTC CACTGTTCAC GCACGCAAAC TGGATGAAAG TGAGGTGTTA 
ATCGTCAGTC CGTCCTACGC GGATGATGAC TCGGCCTATA ATACGGCATC TGGCAGTCAT 
AGCCACGATC ACAGTGACCA TCTGATGGAC ACGAGCCACG TCTCCGTGGT CACCGTGGGC 
GATGAGGGAG TTAAGGTGAA GGACAGCAGC AATGAGCTGG TCAAGCGTCA GCCCAATGGA 
GTTGGCATTG TGCCGGAAGA CGTAAACATA ATTGTAAACC GATTCAAACA GGAGAAGCGG 
TCGCCGGACA GCTCGCTGAG CGAGAATGGC TCGGTGCGCG GACGGCGTGG TATTGAGGTG 
CTGGTTGGCG GCAGCGGAGG ATCCGACGTG GACAGCATTG GCACAAACAC TAGTCAGGAA 
AGCCGGCACG AAACGGATCA CAACAACAAG CAGCAGTATC CAGCGGCCGC GCTGATGCCT 
CCACCACCTC CCTCGTTGAC GAATAACCAC AATCACGAGA CCATTGATGA GGAAGAGGAG 
GTGGTGATCC GACCCAAGGC TAGGGTTCCG GCCGTAGTTA AGTCTGCAAA TGCTCAAGGT 
CTTACCAAGG AAGAGATTGA GCTGCGCAAT CTGCGCAAGA AGACACGCAA GCGCACCCGC 
AAATTCGAGA TCGATGGCGT GCAAATGACC ACCACCACAA GCCGAGTGAT CTACGGGGAC 
GATGAGAACG GACGAATCTA CGATGATCAC GACTTCCGTA AGCAGGAGCT ACGTGAATTA 
AAGCTGCTGC AGAAGCAGGA GAAGAAACAG CAGACGGAAC TGCACTTAAA GGAGCAGCAG 
GCCAAGGAAC AGCAGGATCG TCGCTTTGAG CAGGAGCGCT CCTCGCTGGA AAAGACCTAC 
GAAGCTGACA TGGATATGTT GGCGCGACAG CACAAGCAGC TGGTGGAAAA GACAGAGCAG 
ACGCAGGAGA ACGAGCTGCG CTCCTCATCT AAACGCATTC GCTCCGAGCA GGAGCAAGAG 
TTGAAGATCT TCCGAGAGAA TCTCAAGCAG GAGATCCGAC TGCTCAAGCA GGAAGTTGAC 
CTGTTTCCCA AGGACAAGCG CAAGGATGAG TTCAAGCAGC GGCGCTCAGC CATGGAACTA 
GACCACGAAG AGAAGGAACG CGCCTTCCTG GATTCTTTAA AGGAGAGGCA TGAGCTTCTA 
CTAAGGCGAT TAAGCGAGAA GCATCGGGAC CACCTGGCCA CCATCAACCG CAACTTCTTG 
CAGCAGAAAC AGAACGCCAT GAGGACGCGG GAGGCACTTC TCTGGGAACT AGAGGAGAAG 
CAACTTCACG AACGACATCA GTTGTCCAAG CGGCATGTAA AGGAACTATG CTTCATGCAG 
CGACATCAGA TGATAATCCG GCACGAGAAG GAGCTGGACC AGGTGAAGCG TATGTTGCAG 
CGTAAGGAGG AGGACATGGT CAAGAAACAG ACGATGGAAA AGCGGGCGCT GCCAAAGCGC 
ATCCGTGCGG AACGCAAGGC GCGAGATCTT ATGTTCCGTG AATCCCTTCG AATTTCCACG 
AACCTAGATC CTGAAATCGA ACGCGATCGC TTGAAGAAGT TCCAGGAGCA AGAGAAAAAG 
CGTTATATGC AGGAGGAGCG ACGCTTCGAA GTCAAGCATC AAAAGCAATT GGAAGAGCTA 
CGCGCTACAC GCGAAAGCGC CATAAAAGAG CTGGAGCAAC TTCAGAACGA AAAGCGAAGG 
GCTCTGGTGG AGCACGAACA CTCCAAGCTT TCCGAGATCG ATGAGCGACT GAAGGGGGAG 
CTGCGCGAGT GGCGCGAACA GTTGGTGCCC CGTAAGCAGG AGCTGAATCG ACAGATCAAA 
CTGGCCATTG ATCAGCACGA AAAGCGTTTT GGTCTTGTGA CCAATCGCGA GGAGTTCGAG 
GATCAGGAGG TCAAGCTGCC CGCCCACTTG CGAAACATTT ACAACGAGCG ATCCTCACGC 
ATTCTGCCCC GCAACACCTT TATCGACCCT CAAAACATGC CGCGCTCTCG CTCCTCGCTC 
CTAATGATGG GGGGTGGTGG CAACCGACTG TCCAACTTCC GCGGATCAGC TCCAGATCTT 
AGCCGCAGTG TGCCCAACAC TCCTATTATG CACAAACAAC AGAGATCGCA GATGATAGGC 
GATCAGGTGC TAGAAGAGGA CGAGGAGCAG ATGCTGAGCG AGCAGATGAA GCGGGCCAGC 
GTCGCCACGC TTCCGGCGCA AAACCAACTT CGCCTTATTA CTCAGCTTCC GGCAAACGAG 
CTCGTGAAGG TAGTGACCTC CACTCCACCA CTTAACAGCG CGGAGGATGT GAACTCCAAG 
CCATCCATGA GCACATTCAG AGGCAGTACG CCGCCCAAAA CACCAGATGA TCTTGGCTTG 
GTCACCAATC GATTCAGCCG CCTGGACACC AAAGCTGCAG CGGAGGCCAG CGGCGTGGAG 
TTGCGCATTC ACGAGGGACC AGTGCTAACC GCTCACACTC AGCGGCTGCT GCACACCACC 
TCCTTTGCTA TCAAGCGACC CTCACTGGCC AGCCGGAGCA GCGGCAGATC CTCCCTGAGC 
AGTGCGCAGT CCATGTACAA CATAAATGAG CTGTCCACGC GGTTCACCAG TGATGCGGAC 
CTGATCTTTG ACTCCGGACG CAAGGCTGCC GCCCTGCTAG ACGAAGTGCA ACCGCAGCTA 
CGGTCCTCCG TTAAGCCAGG ACATAGTCGC GCCAATGGAA GCGTCGTCTC AGCCGACTCG 
GCCGCCTCTT CCGAGGATAT CTACTACGAC CTGTATGCGG CGCAGTACAA ACTGCCGAGA 
CAACTACAGC AACGGCTGCA ACAAGAGGTT AGTCCTTTGA TCTATCCCCA GTGTGCTTCC 
CAGCCATTCG ATACGACCCA GGGCCCTGAA ACTGGAGCTC GCCGCCTTGA GGAAACCTTC 
GCCCAACAGC TGGACGAGAT GGAGACCCTG TACGGCGGCG CCCTGATTGT GTCCATGCCC 
TCGGACACGC TGCAGCGAGA TCACTTCACC GGCTCGACGC GCAGCAGCCT CAGCTCGTAT 
TCCGAGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slik ( NM_206213.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995935.1 Drosophila melanogaster Sterile20-like kinase (Slik), transcript variant D, mRNA.

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CloneID	OFa11161
Clone ID Related Accession (Same CDS sequence)	NM_166669.4 , NM_206213.3
Accession Version	NM_206213.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Sterile20-like kinase, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_206213.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTTTA TCACCAACCT GAAGAAGGTC TTCCACCTCG GCGGGGGTGA GGCCAAGAAG 
AAGCGCCTGT ACAACAACAT CAAGATGGAC ACGGATCCGG CGGAGTTCTG GGAAATGGTC 
GGCGAACTGG GCGACGGAGC CTTTGGCAAG GTTTACAAGG CTCAGCACAA GGAGCAGAAG 
CGATTTGCTG CCGCAAAAAT GTGTCAGCTG GAGGACGAGG AGAACCTTAG CGATCACATG 
GTCGAGATCG ACATTCTGTC GGAGATCAAG CACCCAAATA TAGTGGAGCT GTATGAGGCC 
TTTTCCATCG ATGATAAGCT GTGGATGCTC ATCGAGTACT GCGACGGAGG GGCCCTGGAT 
AGCATTATGG TGGAGCTGGA AAAGCCGCTG ACTGAGCCGC AGATTGCCTA TGTTTGCAAG 
CACATGACCG AGGGTCTCAC CTTCCTGCAT CGCAACAAAG TCATCCATCG TGATTTGAAG 
GCAGGAAACG TGCTTCTCAC AATGGAAGGT GGCGTCAAGT TGGCGGACTT TGGCGTTTCG 
GCCAAAAACA AGCACACCAT GCAAAAGCAC GACACCTTCA TTGGCACTCC CTACTGGATG 
GCCCCGGAGC TGGTGCTTTG CGAAACTTTC CGGGACAACC CGTACGATCA CAAAGTGGAC 
ATCTGGTCAC TGGGCATCAC GCTCATTGAG CTGGCGCAGA TGGAGCCGCC CAACAGCGAG 
ATGTCGCCCA TGCGAGTGCT CTTGAAAATA CAAAAGAGCG AGCCGCCCAA GCTGGAGCAG 
CCTTCTCGAT GGAGCAAGGA GTTTAACGAC TTCCTGAAGA AGTCCTTAGT CAAAGATCCG 
CAGGTGAGGC CCACGACGGA TGTGCTGATG CAGCACGCTT TCATCAACAG AAATCTGGAT 
GCCAAGCCTA TCAAGGATCT GCTGCTTGAG TACAAGGCCG AGGTCGTGGA AGAGGTGGTG 
GATGATGAGA CCGAGGAACC CCGCAACTCG GCGCTCCAGC TCGACCTGGA CGATGACTCT 
GCTTCGCTGC AGAGCCAAGA CATTGACAAA CTTCCAGGTA CACCTACATC CATTTTGCGG 
GATGCCAAAG AGCAGTCCCA ACCAAGTAGC AGTTTACCAA TCGCAGCAGC GGCAACTGCA 
GCAGCAGCAG CTACAACCAC AACTAAAGCA ACCACTCCGG ACAGACCAAA CCACACAAAG 
GATGACAATG CTGAAGCGGC AGCCCAGCAG CCGCCTCATA CCAAAGTGCC TGCTCCAGCG 
CCGCCGTCGT CCCAACAAAC GCCACCACCG CAAGTTCAGC AGCCACCTAC ACCACCAGCC 
CAACCCACAG CGGCTGTGCT GCAAAAGAAA CCCGAAGATG TGGCAGCTGT TGCAGAAACA 
TCTGAGAAAA CCGAATCCGA CAAGAAGCAC TTTGTCAAAA AGGGTAAGGC CCCGCCCCCG 
CCGTCCCCAT TGGGTCTTGC AAATGCCAAG CCGGCTGCCA GCGACTCCCA GACGTCTCCC 
AAAAAACTGG CCACTCCCGA GCCCACTTCT CCAGTCACCA CGGCTATTGA GGTGGCCATT 
GGCCAAGAAG CGATGGAGCC CAAACCGCAG CCGCCATCGC CCACAGCCTC CTCCATTGTG 
TCTGTGCAAT CGGTGGCCTC TTCCAGCTCT TCGGGCAGTG TTTCAAATGC TGTTCTCAGC 
TCGAGCACTT CCCTTATTAC CATCAACAGC GATCCACCCA CACCGCATCA TCACCAACCG 
CTGCCACCTC AACCGCAGCA CTTGATTTTG CCAAACAGCT TGGAGTCGGT GAGTCAGATA 
ACAGTCGTGA CCAGCACCCA TCCGCCGGTA ATCATCGACA ACTCGGTGAT GCCACCGCAA 
AACGAGGTGA TCATCGTATC CAATGATATG AACAAGAGCA CTCACCTGCA TGAATCTTCG 
ACGGACGATG ATTTTCCATC GCTGGACGAC AGTTTGGGTG ATGCCACCAC GCCGCCCCAC 
AAGCAATCCT CAATGATATT GGCTGTTAAT GAGCCAGCAG GTGTGGTTCC TGCTCCTCCG 
TCGCAACCGC AAACGTCTTC CACTGTTCAC GCACGCAAAC TGGATGAAAG TGAGGTGTTA 
ATCGTCAGTC CGTCCTACGC GGATGATGAC TCGGCCTATA ATACGGCATC TGGCAGTCAT 
AGCCACGATC ACAGTGACCA TCTGATGGAC ACGAGCCACG TCTCCGTGGT CACCGTGGGC 
GATGAGGGAG TTAAGGTGAA GGACAGCAGC AATGAGCTGG TCAAGCGTCA GCCCAATGGA 
GTTGGCATTG TGCCGGAAGA CGTAAACATA ATTGTAAACC GATTCAAACA GGAGAAGCGG 
TCGCCGGACA GCTCGCTGAG CGAGAATGGC TCGGTGCGCG GACGGCGTGG TATTGAGGTG 
CTGGTTGGCG GCAGCGGAGG ATCCGACGTG GACAGCATTG GCACAAACAC TAGTCAGGAA 
AGCCGGCACG AAACGGATCA CAACAACAAG CAGCAGTATC CAGCGGCCGC GCTGATGCCT 
CCACCACCTC CCTCGTTGAC GAATAACCAC AATCACGAGA CCATTGATGA GGAAGAGGAG 
GTGGTGATCC GACCCAAGGC TAGGGTTCCG GCCGTAGTTA AGTCTGCAAA TGCTCAAGGT 
CTTACCAAGG AAGAGATTGA GCTGCGCAAT CTGCGCAAGA AGACACGCAA GCGCACCCGC 
AAATTCGAGA TCGATGGCGT GCAAATGACC ACCACCACAA GCCGAGTGAT CTACGGGGAC 
GATGAGAACG GACGAATCTA CGATGATCAC GACTTCCGTA AGCAGGAGCT ACGTGAATTA 
AAGCTGCTGC AGAAGCAGGA GAAGAAACAG CAGACGGAAC TGCACTTAAA GGAGCAGCAG 
GCCAAGGAAC AGCAGGATCG TCGCTTTGAG CAGGAGCGCT CCTCGCTGGA AAAGACCTAC 
GAAGCTGACA TGGATATGTT GGCGCGACAG CACAAGCAGC TGGTGGAAAA GACAGAGCAG 
ACGCAGGAGA ACGAGCTGCG CTCCTCATCT AAACGCATTC GCTCCGAGCA GGAGCAAGAG 
TTGAAGATCT TCCGAGAGAA TCTCAAGCAG GAGATCCGAC TGCTCAAGCA GGAAGTTGAC 
CTGTTTCCCA AGGACAAGCG CAAGGATGAG TTCAAGCAGC GGCGCTCAGC CATGGAACTA 
GACCACGAAG AGAAGGAACG CGCCTTCCTG GATTCTTTAA AGGAGAGGCA TGAGCTTCTA 
CTAAGGCGAT TAAGCGAGAA GCATCGGGAC CACCTGGCCA CCATCAACCG CAACTTCTTG 
CAGCAGAAAC AGAACGCCAT GAGGACGCGG GAGGCACTTC TCTGGGAACT AGAGGAGAAG 
CAACTTCACG AACGACATCA GTTGTCCAAG CGGCATGTAA AGGAACTATG CTTCATGCAG 
CGACATCAGA TGATAATCCG GCACGAGAAG GAGCTGGACC AGGTGAAGCG TATGTTGCAG 
CGTAAGGAGG AGGACATGGT CAAGAAACAG ACGATGGAAA AGCGGGCGCT GCCAAAGCGC 
ATCCGTGCGG AACGCAAGGC GCGAGATCTT ATGTTCCGTG AATCCCTTCG AATTTCCACG 
AACCTAGATC CTGAAATCGA ACGCGATCGC TTGAAGAAGT TCCAGGAGCA AGAGAAAAAG 
CGTTATATGC AGGAGGAGCG ACGCTTCGAA GTCAAGCATC AAAAGCAATT GGAAGAGCTA 
CGCGCTACAC GCGAAAGCGC CATAAAAGAG CTGGAGCAAC TTCAGAACGA AAAGCGAAGG 
GCTCTGGTGG AGCACGAACA CTCCAAGCTT TCCGAGATCG ATGAGCGACT GAAGGGGGAG 
CTGCGCGAGT GGCGCGAACA GTTGGTGCCC CGTAAGCAGG AGCTGAATCG ACAGATCAAA 
CTGGCCATTG ATCAGCACGA AAAGCGTTTT GGTCTTGTGA CCAATCGCGA GGAGTTCGAG 
GATCAGGAGG TCAAGCTGCC CGCCCACTTG CGAAACATTT ACAACGAGCG ATCCTCACGC 
ATTCTGCCCC GCAACACCTT TATCGACCCT CAAAACATGC CGCGCTCTCG CTCCTCGCTC 
CTAATGATGG GGGGTGGTGG CAACCGACTG TCCAACTTCC GCGGATCAGC TCCAGATCTT 
AGCCGCAGTG TGCCCAACAC TCCTATTATG CACAAACAAC AGAGATCGCA GATGATAGGC 
GATCAGGTGC TAGAAGAGGA CGAGGAGCAG ATGCTGAGCG AGCAGATGAA GCGGGCCAGC 
GTCGCCACGC TTCCGGCGCA AAACCAACTT CGCCTTATTA CTCAGCTTCC GGCAAACGAG 
CTCGTGAAGG TAGTGACCTC CACTCCACCA CTTAACAGCG CGGAGGATGT GAACTCCAAG 
CCATCCATGA GCACATTCAG AGGCAGTACG CCGCCCAAAA CACCAGATGA TCTTGGCTTG 
GTCACCAATC GATTCAGCCG CCTGGACACC AAAGCTGCAG CGGAGGCCAG CGGCGTGGAG 
TTGCGCATTC ACGAGGGACC AGTGCTAACC GCTCACACTC AGCGGCTGCT GCACACCACC 
TCCTTTGCTA TCAAGCGACC CTCACTGGCC AGCCGGAGCA GCGGCAGATC CTCCCTGAGC 
AGTGCGCAGT CCATGTACAA CATAAATGAG CTGTCCACGC GGTTCACCAG TGATGCGGAC 
CTGATCTTTG ACTCCGGACG CAAGGCTGCC GCCCTGCTAG ACGAAGTGCA ACCGCAGCTA 
CGGTCCTCCG TTAAGCCAGG ACATAGTCGC GCCAATGGAA GCGTCGTCTC AGCCGACTCG 
GCCGCCTCTT CCGAGGATAT CTACTACGAC CTGTATGCGG CGCAGTACAA ACTGCCGAGA 
CAACTACAGC AACGGCTGCA ACAAGAGGTT AGTCCTTTGA TCTATCCCCA GTGTGCTTCC 
CAGCCATTCG ATACGACCCA GGGCCCTGAA ACTGGAGCTC GCCGCCTTGA GGAAACCTTC 
GCCCAACAGC TGGACGAGAT GGAGACCCTG TACGGCGGCG CCCTGATTGT GTCCATGCCC 
TCGGACACGC TGCAGCGAGA TCACTTCACC GGCTCGACGC GCAGCAGCCT CAGCTCGTAT 
TCCGAGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995935.1
CDS	776..5887
Translation	MSFITNLKKVFHLGGGEAKKKRLYNNIKMDTDPAEFWEMVGELGDGAFGKVYKAQHKEQKRFAAAKMCQLEDEENLSDHMVEIDILSEIKHPNIVELYEAFSIDDKLWMLIEYCDGGALDSIMVELEKPLTEPQIAYVCKHMTEGLTFLHRNKVIHRDLKAGNVLLTMEGGVKLADFGVSAKNKHTMQKHDTFIGTPYWMAPELVLCETFRDNPYDHKVDIWSLGITLIELAQMEPPNSEMSPMRVLLKIQKSEPPKLEQPSRWSKEFNDFLKKSLVKDPQVRPTTDVLMQHAFINRNLDAKPIKDLLLEYKAEVVEEVVDDETEEPRNSALQLDLDDDSASLQSQDIDKLPGTPTSILRDAKEQSQPSSSLPIAAAATAAAAATTTTKATTPDRPNHTKDDNAEAAAQQPPHTKVPAPAPPSSQQTPPPQVQQPPTPPAQPTAAVLQKKPEDVAAVAETSEKTESDKKHFVKKGKAPPPPSPLGLANAKPAASDSQTSPKKLATPEPTSPVTTAIEVAIGQEAMEPKPQPPSPTASSIVSVQSVASSSSSGSVSNAVLSSSTSLITINSDPPTPHHHQPLPPQPQHLILPNSLESVSQITVVTSTHPPVIIDNSVMPPQNEVIIVSNDMNKSTHLHESSTDDDFPSLDDSLGDATTPPHKQSSMILAVNEPAGVVPAPPSQPQTSSTVHARKLDESEVLIVSPSYADDDSAYNTASGSHSHDHSDHLMDTSHVSVVTVGDEGVKVKDSSNELVKRQPNGVGIVPEDVNIIVNRFKQEKRSPDSSLSENGSVRGRRGIEVLVGGSGGSDVDSIGTNTSQESRHETDHNNKQQYPAAALMPPPPPSLTNNHNHETIDEEEEVVIRPKARVPAVVKSANAQGLTKEEIELRNLRKKTRKRTRKFEIDGVQMTTTTSRVIYGDDENGRIYDDHDFRKQELRELKLLQKQEKKQQTELHLKEQQAKEQQDRRFEQERSSLEKTYEADMDMLARQHKQLVEKTEQTQENELRSSSKRIRSEQEQELKIFRENLKQEIRLLKQEVDLFPKDKRKDEFKQRRSAMELDHEEKERAFLDSLKERHELLLRRLSEKHRDHLATINRNFLQQKQNAMRTREALLWELEEKQLHERHQLSKRHVKELCFMQRHQMIIRHEKELDQVKRMLQRKEEDMVKKQTMEKRALPKRIRAERKARDLMFRESLRISTNLDPEIERDRLKKFQEQEKKRYMQEERRFEVKHQKQLEELRATRESAIKELEQLQNEKRRALVEHEHSKLSEIDERLKGELREWREQLVPRKQELNRQIKLAIDQHEKRFGLVTNREEFEDQEVKLPAHLRNIYNERSSRILPRNTFIDPQNMPRSRSSLLMMGGGGNRLSNFRGSAPDLSRSVPNTPIMHKQQRSQMIGDQVLEEDEEQMLSEQMKRASVATLPAQNQLRLITQLPANELVKVVTSTPPLNSAEDVNSKPSMSTFRGSTPPKTPDDLGLVTNRFSRLDTKAAAEASGVELRIHEGPVLTAHTQRLLHTTSFAIKRPSLASRSSGRSSLSSAQSMYNINELSTRFTSDADLIFDSGRKAAALLDEVQPQLRSSVKPGHSRANGSVVSADSAASSEDIYYDLYAAQYKLPRQLQQRLQQEVSPLIYPQCASQPFDTTQGPETGARRLEETFAQQLDEMETLYGGALIVSMPSDTLQRDHFTGSTRSSLSSYSEG

Target ORF information:

RefSeq Version	NM_206213.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Sterile20-like kinase (Slik), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206213.3

ORF Insert Sequence:

ATGTCGTTTA TCACCAACCT GAAGAAGGTC TTCCACCTCG GCGGGGGTGA GGCCAAGAAG 
AAGCGCCTGT ACAACAACAT CAAGATGGAC ACGGATCCGG CGGAGTTCTG GGAAATGGTC 
GGCGAACTGG GCGACGGAGC CTTTGGCAAG GTTTACAAGG CTCAGCACAA GGAGCAGAAG 
CGATTTGCTG CCGCAAAAAT GTGTCAGCTG GAGGACGAGG AGAACCTTAG CGATCACATG 
GTCGAGATCG ACATTCTGTC GGAGATCAAG CACCCAAATA TAGTGGAGCT GTATGAGGCC 
TTTTCCATCG ATGATAAGCT GTGGATGCTC ATCGAGTACT GCGACGGAGG GGCCCTGGAT 
AGCATTATGG TGGAGCTGGA AAAGCCGCTG ACTGAGCCGC AGATTGCCTA TGTTTGCAAG 
CACATGACCG AGGGTCTCAC CTTCCTGCAT CGCAACAAAG TCATCCATCG TGATTTGAAG 
GCAGGAAACG TGCTTCTCAC AATGGAAGGT GGCGTCAAGT TGGCGGACTT TGGCGTTTCG 
GCCAAAAACA AGCACACCAT GCAAAAGCAC GACACCTTCA TTGGCACTCC CTACTGGATG 
GCCCCGGAGC TGGTGCTTTG CGAAACTTTC CGGGACAACC CGTACGATCA CAAAGTGGAC 
ATCTGGTCAC TGGGCATCAC GCTCATTGAG CTGGCGCAGA TGGAGCCGCC CAACAGCGAG 
ATGTCGCCCA TGCGAGTGCT CTTGAAAATA CAAAAGAGCG AGCCGCCCAA GCTGGAGCAG 
CCTTCTCGAT GGAGCAAGGA GTTTAACGAC TTCCTGAAGA AGTCCTTAGT CAAAGATCCG 
CAGGTGAGGC CCACGACGGA TGTGCTGATG CAGCACGCTT TCATCAACAG AAATCTGGAT 
GCCAAGCCTA TCAAGGATCT GCTGCTTGAG TACAAGGCCG AGGTCGTGGA AGAGGTGGTG 
GATGATGAGA CCGAGGAACC CCGCAACTCG GCGCTCCAGC TCGACCTGGA CGATGACTCT 
GCTTCGCTGC AGAGCCAAGA CATTGACAAA CTTCCAGGTA CACCTACATC CATTTTGCGG 
GATGCCAAAG AGCAGTCCCA ACCAAGTAGC AGTTTACCAA TCGCAGCAGC GGCAACTGCA 
GCAGCAGCAG CTACAACCAC AACTAAAGCA ACCACTCCGG ACAGACCAAA CCACACAAAG 
GATGACAATG CTGAAGCGGC AGCCCAGCAG CCGCCTCATA CCAAAGTGCC TGCTCCAGCG 
CCGCCGTCGT CCCAACAAAC GCCACCACCG CAAGTTCAGC AGCCACCTAC ACCACCAGCC 
CAACCCACAG CGGCTGTGCT GCAAAAGAAA CCCGAAGATG TGGCAGCTGT TGCAGAAACA 
TCTGAGAAAA CCGAATCCGA CAAGAAGCAC TTTGTCAAAA AGGGTAAGGC CCCGCCCCCG 
CCGTCCCCAT TGGGTCTTGC AAATGCCAAG CCGGCTGCCA GCGACTCCCA GACGTCTCCC 
AAAAAACTGG CCACTCCCGA GCCCACTTCT CCAGTCACCA CGGCTATTGA GGTGGCCATT 
GGCCAAGAAG CGATGGAGCC CAAACCGCAG CCGCCATCGC CCACAGCCTC CTCCATTGTG 
TCTGTGCAAT CGGTGGCCTC TTCCAGCTCT TCGGGCAGTG TTTCAAATGC TGTTCTCAGC 
TCGAGCACTT CCCTTATTAC CATCAACAGC GATCCACCCA CACCGCATCA TCACCAACCG 
CTGCCACCTC AACCGCAGCA CTTGATTTTG CCAAACAGCT TGGAGTCGGT GAGTCAGATA 
ACAGTCGTGA CCAGCACCCA TCCGCCGGTA ATCATCGACA ACTCGGTGAT GCCACCGCAA 
AACGAGGTGA TCATCGTATC CAATGATATG AACAAGAGCA CTCACCTGCA TGAATCTTCG 
ACGGACGATG ATTTTCCATC GCTGGACGAC AGTTTGGGTG ATGCCACCAC GCCGCCCCAC 
AAGCAATCCT CAATGATATT GGCTGTTAAT GAGCCAGCAG GTGTGGTTCC TGCTCCTCCG 
TCGCAACCGC AAACGTCTTC CACTGTTCAC GCACGCAAAC TGGATGAAAG TGAGGTGTTA 
ATCGTCAGTC CGTCCTACGC GGATGATGAC TCGGCCTATA ATACGGCATC TGGCAGTCAT 
AGCCACGATC ACAGTGACCA TCTGATGGAC ACGAGCCACG TCTCCGTGGT CACCGTGGGC 
GATGAGGGAG TTAAGGTGAA GGACAGCAGC AATGAGCTGG TCAAGCGTCA GCCCAATGGA 
GTTGGCATTG TGCCGGAAGA CGTAAACATA ATTGTAAACC GATTCAAACA GGAGAAGCGG 
TCGCCGGACA GCTCGCTGAG CGAGAATGGC TCGGTGCGCG GACGGCGTGG TATTGAGGTG 
CTGGTTGGCG GCAGCGGAGG ATCCGACGTG GACAGCATTG GCACAAACAC TAGTCAGGAA 
AGCCGGCACG AAACGGATCA CAACAACAAG CAGCAGTATC CAGCGGCCGC GCTGATGCCT 
CCACCACCTC CCTCGTTGAC GAATAACCAC AATCACGAGA CCATTGATGA GGAAGAGGAG 
GTGGTGATCC GACCCAAGGC TAGGGTTCCG GCCGTAGTTA AGTCTGCAAA TGCTCAAGGT 
CTTACCAAGG AAGAGATTGA GCTGCGCAAT CTGCGCAAGA AGACACGCAA GCGCACCCGC 
AAATTCGAGA TCGATGGCGT GCAAATGACC ACCACCACAA GCCGAGTGAT CTACGGGGAC 
GATGAGAACG GACGAATCTA CGATGATCAC GACTTCCGTA AGCAGGAGCT ACGTGAATTA 
AAGCTGCTGC AGAAGCAGGA GAAGAAACAG CAGACGGAAC TGCACTTAAA GGAGCAGCAG 
GCCAAGGAAC AGCAGGATCG TCGCTTTGAG CAGGAGCGCT CCTCGCTGGA AAAGACCTAC 
GAAGCTGACA TGGATATGTT GGCGCGACAG CACAAGCAGC TGGTGGAAAA GACAGAGCAG 
ACGCAGGAGA ACGAGCTGCG CTCCTCATCT AAACGCATTC GCTCCGAGCA GGAGCAAGAG 
TTGAAGATCT TCCGAGAGAA TCTCAAGCAG GAGATCCGAC TGCTCAAGCA GGAAGTTGAC 
CTGTTTCCCA AGGACAAGCG CAAGGATGAG TTCAAGCAGC GGCGCTCAGC CATGGAACTA 
GACCACGAAG AGAAGGAACG CGCCTTCCTG GATTCTTTAA AGGAGAGGCA TGAGCTTCTA 
CTAAGGCGAT TAAGCGAGAA GCATCGGGAC CACCTGGCCA CCATCAACCG CAACTTCTTG 
CAGCAGAAAC AGAACGCCAT GAGGACGCGG GAGGCACTTC TCTGGGAACT AGAGGAGAAG 
CAACTTCACG AACGACATCA GTTGTCCAAG CGGCATGTAA AGGAACTATG CTTCATGCAG 
CGACATCAGA TGATAATCCG GCACGAGAAG GAGCTGGACC AGGTGAAGCG TATGTTGCAG 
CGTAAGGAGG AGGACATGGT CAAGAAACAG ACGATGGAAA AGCGGGCGCT GCCAAAGCGC 
ATCCGTGCGG AACGCAAGGC GCGAGATCTT ATGTTCCGTG AATCCCTTCG AATTTCCACG 
AACCTAGATC CTGAAATCGA ACGCGATCGC TTGAAGAAGT TCCAGGAGCA AGAGAAAAAG 
CGTTATATGC AGGAGGAGCG ACGCTTCGAA GTCAAGCATC AAAAGCAATT GGAAGAGCTA 
CGCGCTACAC GCGAAAGCGC CATAAAAGAG CTGGAGCAAC TTCAGAACGA AAAGCGAAGG 
GCTCTGGTGG AGCACGAACA CTCCAAGCTT TCCGAGATCG ATGAGCGACT GAAGGGGGAG 
CTGCGCGAGT GGCGCGAACA GTTGGTGCCC CGTAAGCAGG AGCTGAATCG ACAGATCAAA 
CTGGCCATTG ATCAGCACGA AAAGCGTTTT GGTCTTGTGA CCAATCGCGA GGAGTTCGAG 
GATCAGGAGG TCAAGCTGCC CGCCCACTTG CGAAACATTT ACAACGAGCG ATCCTCACGC 
ATTCTGCCCC GCAACACCTT TATCGACCCT CAAAACATGC CGCGCTCTCG CTCCTCGCTC 
CTAATGATGG GGGGTGGTGG CAACCGACTG TCCAACTTCC GCGGATCAGC TCCAGATCTT 
AGCCGCAGTG TGCCCAACAC TCCTATTATG CACAAACAAC AGAGATCGCA GATGATAGGC 
GATCAGGTGC TAGAAGAGGA CGAGGAGCAG ATGCTGAGCG AGCAGATGAA GCGGGCCAGC 
GTCGCCACGC TTCCGGCGCA AAACCAACTT CGCCTTATTA CTCAGCTTCC GGCAAACGAG 
CTCGTGAAGG TAGTGACCTC CACTCCACCA CTTAACAGCG CGGAGGATGT GAACTCCAAG 
CCATCCATGA GCACATTCAG AGGCAGTACG CCGCCCAAAA CACCAGATGA TCTTGGCTTG 
GTCACCAATC GATTCAGCCG CCTGGACACC AAAGCTGCAG CGGAGGCCAG CGGCGTGGAG 
TTGCGCATTC ACGAGGGACC AGTGCTAACC GCTCACACTC AGCGGCTGCT GCACACCACC 
TCCTTTGCTA TCAAGCGACC CTCACTGGCC AGCCGGAGCA GCGGCAGATC CTCCCTGAGC 
AGTGCGCAGT CCATGTACAA CATAAATGAG CTGTCCACGC GGTTCACCAG TGATGCGGAC 
CTGATCTTTG ACTCCGGACG CAAGGCTGCC GCCCTGCTAG ACGAAGTGCA ACCGCAGCTA 
CGGTCCTCCG TTAAGCCAGG ACATAGTCGC GCCAATGGAA GCGTCGTCTC AGCCGACTCG 
GCCGCCTCTT CCGAGGATAT CTACTACGAC CTGTATGCGG CGCAGTACAA ACTGCCGAGA 
CAACTACAGC AACGGCTGCA ACAAGAGGTT AGTCCTTTGA TCTATCCCCA GTGTGCTTCC 
CAGCCATTCG ATACGACCCA GGGCCCTGAA ACTGGAGCTC GCCGCCTTGA GGAAACCTTC 
GCCCAACAGC TGGACGAGAT GGAGACCCTG TACGGCGGCG CCCTGATTGT GTCCATGCCC 
TCGGACACGC TGCAGCGAGA TCACTTCACC GGCTCGACGC GCAGCAGCCT CAGCTCGTAT 
TCCGAGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slik ( NM_206214.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995936.2 Drosophila melanogaster Sterile20-like kinase (Slik), transcript variant G, mRNA.

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CloneID	OFa11162
Clone ID Related Accession (Same CDS sequence)	NM_206214.4
Accession Version	NM_206214.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Sterile20-like kinase, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_206214.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTTTA TCACCAACCT GAAGAAGGTC TTCCACCTCG GCGGGGGTGA GGCCAAGAAG 
AAGCGCCTGT ACAACAACAT CAAGATGGAC ACGGATCCGG CGGAGTTCTG GGAAATGGTC 
GGCGAACTGG GCGACGGAGC CTTTGGCAAG GTTTACAAGG CTCAGCACAA GGAGCAGAAG 
CGATTTGCTG CCGCAAAAAT GTGTCAGCTG GAGGACGAGG AGAACCTTAG CGATCACATG 
GTCGAGATCG ACATTCTGTC GGAGATCAAG CACCCAAATA TAGTGGAGCT GTATGAGGCC 
TTTTCCATCG ATGATAAGCT GTGGATGCTC ATCGAGTACT GCGACGGAGG GGCCCTGGAT 
AGCATTATGG TGGAGCTGGA AAAGCCGCTG ACTGAGCCGC AGATTGCCTA TGTTTGCAAG 
CACATGACCG AGGGTCTCAC CTTCCTGCAT CGCAACAAAG TCATCCATCG TGATTTGAAG 
GCAGGAAACG TGCTTCTCAC AATGGAAGGT GGCGTCAAGT TGGCGGACTT TGGCGTTTCG 
GCCAAAAACA AGCACACCAT GCAAAAGCAC GACACCTTCA TTGGCACTCC CTACTGGATG 
GCCCCGGAGC TGGTGCTTTG CGAAACTTTC CGGGACAACC CGTACGATCA CAAAGTGGAC 
ATCTGGTCAC TGGGCATCAC GCTCATTGAG CTGGCGCAGA TGGAGCCGCC CAACAGCGAG 
ATGTCGCCCA TGCGAGTGCT CTTGAAAATA CAAAAGAGCG AGCCGCCCAA GCTGGAGCAG 
CCTTCTCGAT GGAGCAAGGA GTTTAACGAC TTCCTGAAGA AGTCCTTAGT CAAAGATCCG 
CAGGTGAGGC CCACGACGGA TGTGCTGATG CAGCACGCTT TCATCAACAG AAATCTGGAT 
GCCAAGCCTA TCAAGGATCT GCTGCTTGAG TACAAGGCCG AGGTCGTGGA AGAGGTGGTG 
GATGATGAGA CCGAGAAGCT TAAACGCCAA GTGAAGCGGC GCTCGCTCTA TCAGAGGGAA 
CCCCGCAACT CGGCGCTCCA GCTCGACCTG GACGATGACT CTGCTTCGCT GCAGAGCCAA 
GACATTGACA AACTTCCAGG TACACCTACA TCCATTTTGC GGGATGCCAA AGAGCAGTCC 
CAACCAAGTA GCAGTTTACC AATCGCAGCA GCGGCAACTG CAGCAGCAGC AGCTACAACC 
ACAACTAAAG CAACCACTCC GGACAGACCA AACCACACAA AGGATGACAA TGCTGAAGCG 
GCAGCCCAGC AGCCGCCTCA TACCAAAGTG CCTGCTCCAG CGCCGCCGTC GTCCCAACAA 
ACGCCACCAC CGCAAGTTCA GCAGCCACCT ACACCACCAG CCCAACCCAC AGCGGCTGTG 
CTGCAAAAGA AACCCGAAGA TGTGGCAGCT GTTGCAGAAA CATCTGAGAA AACCGAATCC 
GACAAGAAGC ACTTTGTCAA AAAGGGTAAG GCCCCGCCCC CGCCGTCCCC ATTGGGTCTT 
GCAAATGCCA AGCCGGCTGC CAGCGACTCC CAGACGTCTC CCAAAAAACT GGCCACTCCC 
GAGCCCACTT CTCCAGTCAC CACGGCTATT GAGGTGGCCA TTGGCCAAGA AGCGATGGAG 
CCCAAACCGC AGCCGCCATC GCCCACAGCC TCCTCCATTG TGTCTGTGCA ATCGGTGGCC 
TCTTCCAGCT CTTCGGGCAG TGTTTCAAAT GCTGTTCTCA GCTCGAGCAC TTCCCTTATT 
ACCATCAACA GCGATCCACC CACACCGCAT CATCACCAAC CGCTGCCACC TCAACCGCAG 
CACTTGATTT TGCCAAACAG CTTGGAGTCG GTGAGTCAGA TAACAGTCGT GACCAGCACC 
CATCCGCCGG TAATCATCGA CAACTCGGTG ATGCCACCGC AAAACGAGGT GATCATCGTA 
TCCAATGATA TGAACAAGAG CACTCACCTG CATGAATCTT CGACGGACGA TGATTTTCCA 
TCGCTGGACG ACAGTTTGGG TGATGCCACC ACGCCGCCCC ACAAGCAATC CTCAATGATA 
TTGGCTGTTA ATGAGCCAGC AGGTGTGGTT CCTGCTCCTC CGTCGCAACC GCAAACGTCT 
TCCACTGTTC ACGCACGCAA ACTGGATGAA AGTGAGGTGT TAATCGTCAG TCCGTCCTAC 
GCGGATGATG ACTCGGCCTA TAATACGGCA TCTGGCAGTC ATAGCCACGA TCACAGTGAC 
CATCTGATGG ACACGAGCCA CGTCTCCGTG GTCACCGTGG GCGATGAGGG AGTTAAGGTG 
AAGGACAGCA GCAATGAGCT GGTCAAGCGT CAGCCCAATG GAGTTGGCAT TGTGCCGGAA 
GACGTAAACA TAATTGTAAA CCGATTCAAA CAGGAGAAGC GGTCGCCGGA CAGCTCGCTG 
AGCGAGAATG GCTCGGTGCG CGGACGGCGT GGTATTGAGG TGCTGGTTGG CGGCAGCGGA 
GGATCCGACG TGGACAGCAT TGGCACAAAC ACTAGTCAGG AAAGCCGGCA CGAAACGGAT 
CACAACAACA AGCAGCAGTA TCCAGCGGCC GCGCTGATGC CTCCACCACC TCCCTCGTTG 
ACGAATAACC ACAATCACGA GACCATTGAT GAGGAAGAGG AGGTGGTGAT CCGACCCAAG 
GCTAGGGTTC CGGCCGTAGT TAAGTCTGCA AATGCTCAAG GTCTTACCAA GGAAGAGATT 
GAGCTGCGCA ATCTGCGCAA GAAGACACGC AAGCGCACCC GCAAATTCGA GATCGATGGC 
GTGCAAATGA CCACCACCAC AAGCCGAGTG ATCTACGGGG ACGATGAGAA CGGACGAATC 
TACGATGATC ACGACTTCCG TAAGCAGGAG CTACGTGAAT TAAAGCTGCT GCAGAAGCAG 
GAGAAGAAAC AGCAGACGGA ACTGCACTTA AAGGAGCAGC AGGCCAAGGA ACAGCAGGAT 
CGTCGCTTTG AGCAGGAGCG CTCCTCGCTG GAAAAGACCT ACGAAGCTGA CATGGATATG 
TTGGCGCGAC AGCACAAGCA GCTGGTGGAA AAGACAGAGC AGACGCAGGA GAACGAGCTG 
CGCTCCTCAT CTAAACGCAT TCGCTCCGAG CAGGAGCAAG AGTTGAAGAT CTTCCGAGAG 
AATCTCAAGC AGGAGATCCG ACTGCTCAAG CAGGAAGTTG ACCTGTTTCC CAAGGACAAG 
CGCAAGGATG AGTTCAAGCA GCGGCGCTCA GCCATGGAAC TAGACCACGA AGAGAAGGAA 
CGCGCCTTCC TGGATTCTTT AAAGGAGAGG CATGAGCTTC TACTAAGGCG ATTAAGCGAG 
AAGCATCGGG ACCACCTGGC CACCATCAAC CGCAACTTCT TGCAGCAGAA ACAGAACGCC 
ATGAGGACGC GGGAGGCACT TCTCTGGGAA CTAGAGGAGA AGCAACTTCA CGAACGACAT 
CAGTTGTCCA AGCGGCATGT AAAGGAACTA TGCTTCATGC AGCGACATCA GATGATAATC 
CGGCACGAGA AGGAGCTGGA CCAGGTGAAG CGTATGTTGC AGCGTAAGGA GGAGGACATG 
GTCAAGAAAC AGACGATGGA AAAGCGGGCG CTGCCAAAGC GCATCCGTGC GGAACGCAAG 
GCGCGAGATC TTATGTTCCG TGAATCCCTT CGAATTTCCA CGAACCTAGA TCCTGAAATC 
GAACGCGATC GCTTGAAGAA GTTCCAGGAG CAAGAGAAAA AGCGTTATAT GCAGGAGGAG 
CGACGCTTCG AAGTCAAGCA TCAAAAGCAA TTGGAAGAGC TACGCGCTAC ACGCGAAAGC 
GCCATAAAAG AGCTGGAGCA ACTTCAGAAC GAAAAGCGAA GGGCTCTGGT GGAGCACGAA 
CACTCCAAGC TTTCCGAGAT CGATGAGCGA CTGAAGGGGG AGCTGCGCGA GTGGCGCGAA 
CAGTTGGTGC CCCGTAAGCA GCAACTACAG CAACGGCTGC AACAAGAGCG CCTTGAGGAA 
ACCTTCGCCC AACAGCTGGA CGAGATGGAG ACCCTGTACG GCGGCGCCCT GATTGTGTCC 
ATGCCCTCGG ACACGCTGCA GCGAGATCAC TTCACCGGCT CGACGCGCAG CAGCCTCAGC 
TCGTATTCCG AGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995936.2
CDS	383..4480
Translation	MSFITNLKKVFHLGGGEAKKKRLYNNIKMDTDPAEFWEMVGELGDGAFGKVYKAQHKEQKRFAAAKMCQLEDEENLSDHMVEIDILSEIKHPNIVELYEAFSIDDKLWMLIEYCDGGALDSIMVELEKPLTEPQIAYVCKHMTEGLTFLHRNKVIHRDLKAGNVLLTMEGGVKLADFGVSAKNKHTMQKHDTFIGTPYWMAPELVLCETFRDNPYDHKVDIWSLGITLIELAQMEPPNSEMSPMRVLLKIQKSEPPKLEQPSRWSKEFNDFLKKSLVKDPQVRPTTDVLMQHAFINRNLDAKPIKDLLLEYKAEVVEEVVDDETEKLKRQVKRRSLYQREPRNSALQLDLDDDSASLQSQDIDKLPGTPTSILRDAKEQSQPSSSLPIAAAATAAAAATTTTKATTPDRPNHTKDDNAEAAAQQPPHTKVPAPAPPSSQQTPPPQVQQPPTPPAQPTAAVLQKKPEDVAAVAETSEKTESDKKHFVKKGKAPPPPSPLGLANAKPAASDSQTSPKKLATPEPTSPVTTAIEVAIGQEAMEPKPQPPSPTASSIVSVQSVASSSSSGSVSNAVLSSSTSLITINSDPPTPHHHQPLPPQPQHLILPNSLESVSQITVVTSTHPPVIIDNSVMPPQNEVIIVSNDMNKSTHLHESSTDDDFPSLDDSLGDATTPPHKQSSMILAVNEPAGVVPAPPSQPQTSSTVHARKLDESEVLIVSPSYADDDSAYNTASGSHSHDHSDHLMDTSHVSVVTVGDEGVKVKDSSNELVKRQPNGVGIVPEDVNIIVNRFKQEKRSPDSSLSENGSVRGRRGIEVLVGGSGGSDVDSIGTNTSQESRHETDHNNKQQYPAAALMPPPPPSLTNNHNHETIDEEEEVVIRPKARVPAVVKSANAQGLTKEEIELRNLRKKTRKRTRKFEIDGVQMTTTTSRVIYGDDENGRIYDDHDFRKQELRELKLLQKQEKKQQTELHLKEQQAKEQQDRRFEQERSSLEKTYEADMDMLARQHKQLVEKTEQTQENELRSSSKRIRSEQEQELKIFRENLKQEIRLLKQEVDLFPKDKRKDEFKQRRSAMELDHEEKERAFLDSLKERHELLLRRLSEKHRDHLATINRNFLQQKQNAMRTREALLWELEEKQLHERHQLSKRHVKELCFMQRHQMIIRHEKELDQVKRMLQRKEEDMVKKQTMEKRALPKRIRAERKARDLMFRESLRISTNLDPEIERDRLKKFQEQEKKRYMQEERRFEVKHQKQLEELRATRESAIKELEQLQNEKRRALVEHEHSKLSEIDERLKGELREWREQLVPRKQQLQQRLQQERLEETFAQQLDEMETLYGGALIVSMPSDTLQRDHFTGSTRSSLSSYSEG

Target ORF information:

RefSeq Version	NM_206214.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Sterile20-like kinase (Slik), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206214.4

ORF Insert Sequence:

ATGTCGTTTA TCACCAACCT GAAGAAGGTC TTCCACCTCG GCGGGGGTGA GGCCAAGAAG 
AAGCGCCTGT ACAACAACAT CAAGATGGAC ACGGATCCGG CGGAGTTCTG GGAAATGGTC 
GGCGAACTGG GCGACGGAGC CTTTGGCAAG GTTTACAAGG CTCAGCACAA GGAGCAGAAG 
CGATTTGCTG CCGCAAAAAT GTGTCAGCTG GAGGACGAGG AGAACCTTAG CGATCACATG 
GTCGAGATCG ACATTCTGTC GGAGATCAAG CACCCAAATA TAGTGGAGCT GTATGAGGCC 
TTTTCCATCG ATGATAAGCT GTGGATGCTC ATCGAGTACT GCGACGGAGG GGCCCTGGAT 
AGCATTATGG TGGAGCTGGA AAAGCCGCTG ACTGAGCCGC AGATTGCCTA TGTTTGCAAG 
CACATGACCG AGGGTCTCAC CTTCCTGCAT CGCAACAAAG TCATCCATCG TGATTTGAAG 
GCAGGAAACG TGCTTCTCAC AATGGAAGGT GGCGTCAAGT TGGCGGACTT TGGCGTTTCG 
GCCAAAAACA AGCACACCAT GCAAAAGCAC GACACCTTCA TTGGCACTCC CTACTGGATG 
GCCCCGGAGC TGGTGCTTTG CGAAACTTTC CGGGACAACC CGTACGATCA CAAAGTGGAC 
ATCTGGTCAC TGGGCATCAC GCTCATTGAG CTGGCGCAGA TGGAGCCGCC CAACAGCGAG 
ATGTCGCCCA TGCGAGTGCT CTTGAAAATA CAAAAGAGCG AGCCGCCCAA GCTGGAGCAG 
CCTTCTCGAT GGAGCAAGGA GTTTAACGAC TTCCTGAAGA AGTCCTTAGT CAAAGATCCG 
CAGGTGAGGC CCACGACGGA TGTGCTGATG CAGCACGCTT TCATCAACAG AAATCTGGAT 
GCCAAGCCTA TCAAGGATCT GCTGCTTGAG TACAAGGCCG AGGTCGTGGA AGAGGTGGTG 
GATGATGAGA CCGAGAAGCT TAAACGCCAA GTGAAGCGGC GCTCGCTCTA TCAGAGGGAA 
CCCCGCAACT CGGCGCTCCA GCTCGACCTG GACGATGACT CTGCTTCGCT GCAGAGCCAA 
GACATTGACA AACTTCCAGG TACACCTACA TCCATTTTGC GGGATGCCAA AGAGCAGTCC 
CAACCAAGTA GCAGTTTACC AATCGCAGCA GCGGCAACTG CAGCAGCAGC AGCTACAACC 
ACAACTAAAG CAACCACTCC GGACAGACCA AACCACACAA AGGATGACAA TGCTGAAGCG 
GCAGCCCAGC AGCCGCCTCA TACCAAAGTG CCTGCTCCAG CGCCGCCGTC GTCCCAACAA 
ACGCCACCAC CGCAAGTTCA GCAGCCACCT ACACCACCAG CCCAACCCAC AGCGGCTGTG 
CTGCAAAAGA AACCCGAAGA TGTGGCAGCT GTTGCAGAAA CATCTGAGAA AACCGAATCC 
GACAAGAAGC ACTTTGTCAA AAAGGGTAAG GCCCCGCCCC CGCCGTCCCC ATTGGGTCTT 
GCAAATGCCA AGCCGGCTGC CAGCGACTCC CAGACGTCTC CCAAAAAACT GGCCACTCCC 
GAGCCCACTT CTCCAGTCAC CACGGCTATT GAGGTGGCCA TTGGCCAAGA AGCGATGGAG 
CCCAAACCGC AGCCGCCATC GCCCACAGCC TCCTCCATTG TGTCTGTGCA ATCGGTGGCC 
TCTTCCAGCT CTTCGGGCAG TGTTTCAAAT GCTGTTCTCA GCTCGAGCAC TTCCCTTATT 
ACCATCAACA GCGATCCACC CACACCGCAT CATCACCAAC CGCTGCCACC TCAACCGCAG 
CACTTGATTT TGCCAAACAG CTTGGAGTCG GTGAGTCAGA TAACAGTCGT GACCAGCACC 
CATCCGCCGG TAATCATCGA CAACTCGGTG ATGCCACCGC AAAACGAGGT GATCATCGTA 
TCCAATGATA TGAACAAGAG CACTCACCTG CATGAATCTT CGACGGACGA TGATTTTCCA 
TCGCTGGACG ACAGTTTGGG TGATGCCACC ACGCCGCCCC ACAAGCAATC CTCAATGATA 
TTGGCTGTTA ATGAGCCAGC AGGTGTGGTT CCTGCTCCTC CGTCGCAACC GCAAACGTCT 
TCCACTGTTC ACGCACGCAA ACTGGATGAA AGTGAGGTGT TAATCGTCAG TCCGTCCTAC 
GCGGATGATG ACTCGGCCTA TAATACGGCA TCTGGCAGTC ATAGCCACGA TCACAGTGAC 
CATCTGATGG ACACGAGCCA CGTCTCCGTG GTCACCGTGG GCGATGAGGG AGTTAAGGTG 
AAGGACAGCA GCAATGAGCT GGTCAAGCGT CAGCCCAATG GAGTTGGCAT TGTGCCGGAA 
GACGTAAACA TAATTGTAAA CCGATTCAAA CAGGAGAAGC GGTCGCCGGA CAGCTCGCTG 
AGCGAGAATG GCTCGGTGCG CGGACGGCGT GGTATTGAGG TGCTGGTTGG CGGCAGCGGA 
GGATCCGACG TGGACAGCAT TGGCACAAAC ACTAGTCAGG AAAGCCGGCA CGAAACGGAT 
CACAACAACA AGCAGCAGTA TCCAGCGGCC GCGCTGATGC CTCCACCACC TCCCTCGTTG 
ACGAATAACC ACAATCACGA GACCATTGAT GAGGAAGAGG AGGTGGTGAT CCGACCCAAG 
GCTAGGGTTC CGGCCGTAGT TAAGTCTGCA AATGCTCAAG GTCTTACCAA GGAAGAGATT 
GAGCTGCGCA ATCTGCGCAA GAAGACACGC AAGCGCACCC GCAAATTCGA GATCGATGGC 
GTGCAAATGA CCACCACCAC AAGCCGAGTG ATCTACGGGG ACGATGAGAA CGGACGAATC 
TACGATGATC ACGACTTCCG TAAGCAGGAG CTACGTGAAT TAAAGCTGCT GCAGAAGCAG 
GAGAAGAAAC AGCAGACGGA ACTGCACTTA AAGGAGCAGC AGGCCAAGGA ACAGCAGGAT 
CGTCGCTTTG AGCAGGAGCG CTCCTCGCTG GAAAAGACCT ACGAAGCTGA CATGGATATG 
TTGGCGCGAC AGCACAAGCA GCTGGTGGAA AAGACAGAGC AGACGCAGGA GAACGAGCTG 
CGCTCCTCAT CTAAACGCAT TCGCTCCGAG CAGGAGCAAG AGTTGAAGAT CTTCCGAGAG 
AATCTCAAGC AGGAGATCCG ACTGCTCAAG CAGGAAGTTG ACCTGTTTCC CAAGGACAAG 
CGCAAGGATG AGTTCAAGCA GCGGCGCTCA GCCATGGAAC TAGACCACGA AGAGAAGGAA 
CGCGCCTTCC TGGATTCTTT AAAGGAGAGG CATGAGCTTC TACTAAGGCG ATTAAGCGAG 
AAGCATCGGG ACCACCTGGC CACCATCAAC CGCAACTTCT TGCAGCAGAA ACAGAACGCC 
ATGAGGACGC GGGAGGCACT TCTCTGGGAA CTAGAGGAGA AGCAACTTCA CGAACGACAT 
CAGTTGTCCA AGCGGCATGT AAAGGAACTA TGCTTCATGC AGCGACATCA GATGATAATC 
CGGCACGAGA AGGAGCTGGA CCAGGTGAAG CGTATGTTGC AGCGTAAGGA GGAGGACATG 
GTCAAGAAAC AGACGATGGA AAAGCGGGCG CTGCCAAAGC GCATCCGTGC GGAACGCAAG 
GCGCGAGATC TTATGTTCCG TGAATCCCTT CGAATTTCCA CGAACCTAGA TCCTGAAATC 
GAACGCGATC GCTTGAAGAA GTTCCAGGAG CAAGAGAAAA AGCGTTATAT GCAGGAGGAG 
CGACGCTTCG AAGTCAAGCA TCAAAAGCAA TTGGAAGAGC TACGCGCTAC ACGCGAAAGC 
GCCATAAAAG AGCTGGAGCA ACTTCAGAAC GAAAAGCGAA GGGCTCTGGT GGAGCACGAA 
CACTCCAAGC TTTCCGAGAT CGATGAGCGA CTGAAGGGGG AGCTGCGCGA GTGGCGCGAA 
CAGTTGGTGC CCCGTAAGCA GCAACTACAG CAACGGCTGC AACAAGAGCG CCTTGAGGAA 
ACCTTCGCCC AACAGCTGGA CGAGATGGAG ACCCTGTACG GCGGCGCCCT GATTGTGTCC 
ATGCCCTCGG ACACGCTGCA GCGAGATCAC TTCACCGGCT CGACGCGCAG CAGCCTCAGC 
TCGTATTCCG AGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slik ( NM_001014549.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014549.1 Drosophila melanogaster Sterile20-like kinase (Slik), transcript variant E, mRNA.

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CloneID	OFa11163
Clone ID Related Accession (Same CDS sequence)	NM_001014549.3
Accession Version	NM_001014549.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Sterile20-like kinase, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001014549.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTTTA TCACCAACCT GAAGAAGGTC TTCCACCTCG GCGGGGGTGA GGCCAAGAAG 
AAGCGCCTGT ACAACAACAT CAAGATGGAC ACGGATCCGG CGGAGTTCTG GGAAATGGTC 
GGCGAACTGG GCGACGGAGC CTTTGGCAAG GTTTACAAGG CTCAGCACAA GGAGCAGAAG 
CGATTTGCTG CCGCAAAAAT GTGTCAGCTG GAGGACGAGG AGAACCTTAG CGATCACATG 
GTCGAGATCG ACATTCTGTC GGAGATCAAG CACCCAAATA TAGTGGAGCT GTATGAGGCC 
TTTTCCATCG ATGATAAGCT GTGGATGCTC ATCGAGTACT GCGACGGAGG GGCCCTGGAT 
AGCATTATGG TGGAGCTGGA AAAGCCGCTG ACTGAGCCGC AGATTGCCTA TGTTTGCAAG 
CACATGACCG AGGGTCTCAC CTTCCTGCAT CGCAACAAAG TCATCCATCG TGATTTGAAG 
GCAGGAAACG TGCTTCTCAC AATGGAAGGT GGCGTCAAGT TGGCGGACTT TGGCGTTTCG 
GCCAAAAACA AGCACACCAT GCAAAAGCAC GACACCTTCA TTGGCACTCC CTACTGGATG 
GCCCCGGAGC TGGTGCTTTG CGAAACTTTC CGGGACAACC CGTACGATCA CAAAGTGGAC 
ATCTGGTCAC TGGGCATCAC GCTCATTGAG CTGGCGCAGA TGGAGCCGCC CAACAGCGAG 
ATGTCGCCCA TGCGAGTGCT CTTGAAAATA CAAAAGAGCG AGCCGCCCAA GCTGGAGCAG 
CCTTCTCGAT GGAGCAAGGA GTTTAACGAC TTCCTGAAGA AGTCCTTAGT CAAAGATCCG 
CAGGTGAGGC CCACGACGGA TGTGCTGATG CAGCACGCTT TCATCAACAG AAATCTGGAT 
GCCAAGCCTA TCAAGGATCT GCTGCTTGAG TACAAGGCCG AGGTCGTGGA AGAGGTGGTG 
GATGATGAGA CCGAGGAACC CCGCAACTCG GCGCTCCAGC TCGACCTGGA CGATGACTCT 
GCTTCGCTGC AGAGCCAAGA CATTGACAAA CTTCCAGGTA CACCTACATC CATTTTGCGG 
GATGCCAAAG AGCAGTCCCA ACCAAGTAGC AGTTTACCAA TCGCAGCAGC GGCAACTGCA 
GCAGCAGCAG CTACAACCAC AACTAAAGCA ACCACTCCGG ACAGACCAAA CCACACAAAG 
GATGACAATG CTGAAGCGGC AGCCCAGCAG CCGCCTCATA CCAAAGTGCC TGCTCCAGCG 
CCGCCGTCGT CCCAACAAAC GCCACCACCG CAAGTTCAGC AGCCACCTAC ACCACCAGCC 
CAACCCACAG CGGCTGTGCT GCAAAAGAAA CCCGAAGATG TGGCAGCTGT TGCAGAAACA 
TCTGAGAAAA CCGAATCCGA CAAGAAGCAC TTTGTCAAAA AGGGTAAGGC CCCGCCCCCG 
CCGTCCCCAT TGGGTCTTGC AAATGCCAAG CCGGCTGCCA GCGACTCCCA GACGTCTCCC 
AAAAAACTGG CCACTCCCGA GCCCACTTCT CCAGTCACCA CGGCTATTGA GGTGGCCATT 
GGCCAAGAAG CGATGGAGCC CAAACCGCAG CCGCCATCGC CCACAGCCTC CTCCATTGTG 
TCTGTGCAAT CGGTGGCCTC TTCCAGCTCT TCGGGCAGTG TTTCAAATGC TGTTCTCAGC 
TCGAGCACTT CCCTTATTAC CATCAACAGC GATCCACCCA CACCGCATCA TCACCAACCG 
CTGCCACCTC AACCGCAGCA CTTGATTTTG CCAAACAGCT TGGAGTCGGT GAGTCAGATA 
ACAGTCGTGA CCAGCACCCA TCCGCCGGTA ATCATCGACA ACTCGGTGAT GCCACCGCAA 
AACGAGGTGA TCATCGTATC CAATGATATG AACAAGAGCA CTCACCTGCA TGAATCTTCG 
ACGGACGATG ATTTTCCATC GCTGGACGAC AGTTTGGGTG ATGCCACCAC GCCGCCCCAC 
AAGCAATCCT CAATGATATT GGCTGTTAAT GAGCCAGCAG GTGTGGTTCC TGCTCCTCCG 
TCGCAACCGC AAACGTCTTC CACTGTTCAC GCACGCAAAC TGGATGAAAG TGAGGTGTTA 
ATCGTCAGTC CGTCCTACGC GGATGATGAC TCGGCCTATA ATACGGCATC TGGCAGTCAT 
AGCCACGATC ACAGTGACCA TCTGATGGAC ACGAGCCACG TCTCCGTGGT CACCGTGGGC 
GATGAGGGAG TTAAGGTGAA GGACAGCAGC AATGAGCTGG TCAAGCGTCA GCCCAATGGA 
GTTGGCATTG TGCCGGAAGA CGTAAACATA ATTGTAAACC GATTCAAACA GGAGAAGCGG 
TCGCCGGACA GCTCGCTGAG CGAGAATGGC TCGGTGCGCG GACGGCGTGG TATTGAGGTG 
CTGGTTGGCG GCAGCGGAGG ATCCGACGTG GACAGCATTG GCACAAACAC TAGTCAGGAA 
AGCCGGCACG AAACGGATCA CAACAACAAG CAGCAGTATC CAGCGGCCGC GCTGATGCCT 
CCACCACCTC CCTCGTTGAC GAATAACCAC AATCACGAGA CCATTGATGA GGAAGAGGAG 
GTGGTGATCC GACCCAAGGC TAGGGTTCCG GCCGTAGTTA AGTCTGCAAA TGCTCAAGGT 
CTTACCAAGG AAGAGATTGA GCTGCGCAAT CTGCGCAAGA AGACACGCAA GCGCACCCGC 
AAATTCGAGA TCGATGGCGT GCAAATGACC ACCACCACAA GCCGAGTGAT CTACGGGGAC 
GATGAGAACG GACGAATCTA CGATGATCAC GACTTCCGTA AGCAGGAGCT ACGTGAATTA 
AAGCTGCTGC AGAAGCAGGA GAAGAAACAG CAGACGGAAC TGCACTTAAA GGAGCAGCAG 
GCCAAGGAAC AGCAGGATCG TCGCTTTGAG CAGGAGCGCT CCTCGCTGGA AAAGACCTAC 
GAAGCTGACA TGGATATGTT GGCGCGACAG CACAAGCAGC TGGTGGAAAA GACAGAGCAG 
ACGCAGGAGA ACGAGCTGCG CTCCTCATCT AAACGCATTC GCTCCGAGCA GGAGCAAGAG 
TTGAAGATCT TCCGAGAGAA TCTCAAGCAG GAGATCCGAC TGCTCAAGCA GGAAGTTGAC 
CTGTTTCCCA AGGACAAGCG CAAGGATGAG TTCAAGCAGC GGCGCTCAGC CATGGAACTA 
GACCACGAAG AGAAGGAACG CGCCTTCCTG GATTCTTTAA AGGAGAGGCA TGAGCTTCTA 
CTAAGGCGAT TAAGCGAGAA GCATCGGGAC CACCTGGCCA CCATCAACCG CAACTTCTTG 
CAGCAGAAAC AGAACGCCAT GAGGACGCGG GAGGCACTTC TCTGGGAACT AGAGGAGAAG 
CAACTTCACG AACGACATCA GTTGTCCAAG CGGCATGTAA AGGAACTATG CTTCATGCAG 
CGACATCAGA TGATAATCCG GCACGAGAAG GAGCTGGACC AGGTGAAGCG TATGTTGCAG 
CGTAAGGAGG AGGACATGGT CAAGAAACAG ACGATGGAAA AGCGGGCGCT GCCAAAGCGC 
ATCCGTGCGG AACGCAAGGC GCGAGATCTT ATGTTCCGTG AATCCCTTCG AATTTCCACG 
AACCTAGATC CTGAAATCGA ACGCGATCGC TTGAAGAAGT TCCAGGAGCA AGAGAAAAAG 
CGTTATATGC AGGAGGAGCG ACGCTTCGAA GTCAAGCATC AAAAGCAATT GGAAGAGCTA 
CGCGCTACAC GCGAAAGCGC CATAAAAGAG CTGGAGCAAC TTCAGAACGA AAAGCGAAGG 
GCTCTGGTGG AGCACGAACA CTCCAAGCTT TCCGAGATCG ATGAGCGACT GAAGGGGGAG 
CTGCGCGAGT GGCGCGAACA GTTGGTGCCC CGTAAGCAGC GCCTTGAGGA AACCTTCGCC 
CAACAGCTGG ACGAGATGGA GACCCTGTAC GGCGGCGCCC TGATTGTGTC CATGCCCTCG 
GACACGCTGC AGCGAGATCA CTTCACCGGC TCGACGCGCA GCAGCCTCAG CTCGTATTCC 
GAGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014549.1
CDS	383..4411
Translation	MSFITNLKKVFHLGGGEAKKKRLYNNIKMDTDPAEFWEMVGELGDGAFGKVYKAQHKEQKRFAAAKMCQLEDEENLSDHMVEIDILSEIKHPNIVELYEAFSIDDKLWMLIEYCDGGALDSIMVELEKPLTEPQIAYVCKHMTEGLTFLHRNKVIHRDLKAGNVLLTMEGGVKLADFGVSAKNKHTMQKHDTFIGTPYWMAPELVLCETFRDNPYDHKVDIWSLGITLIELAQMEPPNSEMSPMRVLLKIQKSEPPKLEQPSRWSKEFNDFLKKSLVKDPQVRPTTDVLMQHAFINRNLDAKPIKDLLLEYKAEVVEEVVDDETEEPRNSALQLDLDDDSASLQSQDIDKLPGTPTSILRDAKEQSQPSSSLPIAAAATAAAAATTTTKATTPDRPNHTKDDNAEAAAQQPPHTKVPAPAPPSSQQTPPPQVQQPPTPPAQPTAAVLQKKPEDVAAVAETSEKTESDKKHFVKKGKAPPPPSPLGLANAKPAASDSQTSPKKLATPEPTSPVTTAIEVAIGQEAMEPKPQPPSPTASSIVSVQSVASSSSSGSVSNAVLSSSTSLITINSDPPTPHHHQPLPPQPQHLILPNSLESVSQITVVTSTHPPVIIDNSVMPPQNEVIIVSNDMNKSTHLHESSTDDDFPSLDDSLGDATTPPHKQSSMILAVNEPAGVVPAPPSQPQTSSTVHARKLDESEVLIVSPSYADDDSAYNTASGSHSHDHSDHLMDTSHVSVVTVGDEGVKVKDSSNELVKRQPNGVGIVPEDVNIIVNRFKQEKRSPDSSLSENGSVRGRRGIEVLVGGSGGSDVDSIGTNTSQESRHETDHNNKQQYPAAALMPPPPPSLTNNHNHETIDEEEEVVIRPKARVPAVVKSANAQGLTKEEIELRNLRKKTRKRTRKFEIDGVQMTTTTSRVIYGDDENGRIYDDHDFRKQELRELKLLQKQEKKQQTELHLKEQQAKEQQDRRFEQERSSLEKTYEADMDMLARQHKQLVEKTEQTQENELRSSSKRIRSEQEQELKIFRENLKQEIRLLKQEVDLFPKDKRKDEFKQRRSAMELDHEEKERAFLDSLKERHELLLRRLSEKHRDHLATINRNFLQQKQNAMRTREALLWELEEKQLHERHQLSKRHVKELCFMQRHQMIIRHEKELDQVKRMLQRKEEDMVKKQTMEKRALPKRIRAERKARDLMFRESLRISTNLDPEIERDRLKKFQEQEKKRYMQEERRFEVKHQKQLEELRATRESAIKELEQLQNEKRRALVEHEHSKLSEIDERLKGELREWREQLVPRKQRLEETFAQQLDEMETLYGGALIVSMPSDTLQRDHFTGSTRSSLSSYSEG

Target ORF information:

RefSeq Version	NM_001014549.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Sterile20-like kinase (Slik), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014549.3

ORF Insert Sequence:

ATGTCGTTTA TCACCAACCT GAAGAAGGTC TTCCACCTCG GCGGGGGTGA GGCCAAGAAG 
AAGCGCCTGT ACAACAACAT CAAGATGGAC ACGGATCCGG CGGAGTTCTG GGAAATGGTC 
GGCGAACTGG GCGACGGAGC CTTTGGCAAG GTTTACAAGG CTCAGCACAA GGAGCAGAAG 
CGATTTGCTG CCGCAAAAAT GTGTCAGCTG GAGGACGAGG AGAACCTTAG CGATCACATG 
GTCGAGATCG ACATTCTGTC GGAGATCAAG CACCCAAATA TAGTGGAGCT GTATGAGGCC 
TTTTCCATCG ATGATAAGCT GTGGATGCTC ATCGAGTACT GCGACGGAGG GGCCCTGGAT 
AGCATTATGG TGGAGCTGGA AAAGCCGCTG ACTGAGCCGC AGATTGCCTA TGTTTGCAAG 
CACATGACCG AGGGTCTCAC CTTCCTGCAT CGCAACAAAG TCATCCATCG TGATTTGAAG 
GCAGGAAACG TGCTTCTCAC AATGGAAGGT GGCGTCAAGT TGGCGGACTT TGGCGTTTCG 
GCCAAAAACA AGCACACCAT GCAAAAGCAC GACACCTTCA TTGGCACTCC CTACTGGATG 
GCCCCGGAGC TGGTGCTTTG CGAAACTTTC CGGGACAACC CGTACGATCA CAAAGTGGAC 
ATCTGGTCAC TGGGCATCAC GCTCATTGAG CTGGCGCAGA TGGAGCCGCC CAACAGCGAG 
ATGTCGCCCA TGCGAGTGCT CTTGAAAATA CAAAAGAGCG AGCCGCCCAA GCTGGAGCAG 
CCTTCTCGAT GGAGCAAGGA GTTTAACGAC TTCCTGAAGA AGTCCTTAGT CAAAGATCCG 
CAGGTGAGGC CCACGACGGA TGTGCTGATG CAGCACGCTT TCATCAACAG AAATCTGGAT 
GCCAAGCCTA TCAAGGATCT GCTGCTTGAG TACAAGGCCG AGGTCGTGGA AGAGGTGGTG 
GATGATGAGA CCGAGGAACC CCGCAACTCG GCGCTCCAGC TCGACCTGGA CGATGACTCT 
GCTTCGCTGC AGAGCCAAGA CATTGACAAA CTTCCAGGTA CACCTACATC CATTTTGCGG 
GATGCCAAAG AGCAGTCCCA ACCAAGTAGC AGTTTACCAA TCGCAGCAGC GGCAACTGCA 
GCAGCAGCAG CTACAACCAC AACTAAAGCA ACCACTCCGG ACAGACCAAA CCACACAAAG 
GATGACAATG CTGAAGCGGC AGCCCAGCAG CCGCCTCATA CCAAAGTGCC TGCTCCAGCG 
CCGCCGTCGT CCCAACAAAC GCCACCACCG CAAGTTCAGC AGCCACCTAC ACCACCAGCC 
CAACCCACAG CGGCTGTGCT GCAAAAGAAA CCCGAAGATG TGGCAGCTGT TGCAGAAACA 
TCTGAGAAAA CCGAATCCGA CAAGAAGCAC TTTGTCAAAA AGGGTAAGGC CCCGCCCCCG 
CCGTCCCCAT TGGGTCTTGC AAATGCCAAG CCGGCTGCCA GCGACTCCCA GACGTCTCCC 
AAAAAACTGG CCACTCCCGA GCCCACTTCT CCAGTCACCA CGGCTATTGA GGTGGCCATT 
GGCCAAGAAG CGATGGAGCC CAAACCGCAG CCGCCATCGC CCACAGCCTC CTCCATTGTG 
TCTGTGCAAT CGGTGGCCTC TTCCAGCTCT TCGGGCAGTG TTTCAAATGC TGTTCTCAGC 
TCGAGCACTT CCCTTATTAC CATCAACAGC GATCCACCCA CACCGCATCA TCACCAACCG 
CTGCCACCTC AACCGCAGCA CTTGATTTTG CCAAACAGCT TGGAGTCGGT GAGTCAGATA 
ACAGTCGTGA CCAGCACCCA TCCGCCGGTA ATCATCGACA ACTCGGTGAT GCCACCGCAA 
AACGAGGTGA TCATCGTATC CAATGATATG AACAAGAGCA CTCACCTGCA TGAATCTTCG 
ACGGACGATG ATTTTCCATC GCTGGACGAC AGTTTGGGTG ATGCCACCAC GCCGCCCCAC 
AAGCAATCCT CAATGATATT GGCTGTTAAT GAGCCAGCAG GTGTGGTTCC TGCTCCTCCG 
TCGCAACCGC AAACGTCTTC CACTGTTCAC GCACGCAAAC TGGATGAAAG TGAGGTGTTA 
ATCGTCAGTC CGTCCTACGC GGATGATGAC TCGGCCTATA ATACGGCATC TGGCAGTCAT 
AGCCACGATC ACAGTGACCA TCTGATGGAC ACGAGCCACG TCTCCGTGGT CACCGTGGGC 
GATGAGGGAG TTAAGGTGAA GGACAGCAGC AATGAGCTGG TCAAGCGTCA GCCCAATGGA 
GTTGGCATTG TGCCGGAAGA CGTAAACATA ATTGTAAACC GATTCAAACA GGAGAAGCGG 
TCGCCGGACA GCTCGCTGAG CGAGAATGGC TCGGTGCGCG GACGGCGTGG TATTGAGGTG 
CTGGTTGGCG GCAGCGGAGG ATCCGACGTG GACAGCATTG GCACAAACAC TAGTCAGGAA 
AGCCGGCACG AAACGGATCA CAACAACAAG CAGCAGTATC CAGCGGCCGC GCTGATGCCT 
CCACCACCTC CCTCGTTGAC GAATAACCAC AATCACGAGA CCATTGATGA GGAAGAGGAG 
GTGGTGATCC GACCCAAGGC TAGGGTTCCG GCCGTAGTTA AGTCTGCAAA TGCTCAAGGT 
CTTACCAAGG AAGAGATTGA GCTGCGCAAT CTGCGCAAGA AGACACGCAA GCGCACCCGC 
AAATTCGAGA TCGATGGCGT GCAAATGACC ACCACCACAA GCCGAGTGAT CTACGGGGAC 
GATGAGAACG GACGAATCTA CGATGATCAC GACTTCCGTA AGCAGGAGCT ACGTGAATTA 
AAGCTGCTGC AGAAGCAGGA GAAGAAACAG CAGACGGAAC TGCACTTAAA GGAGCAGCAG 
GCCAAGGAAC AGCAGGATCG TCGCTTTGAG CAGGAGCGCT CCTCGCTGGA AAAGACCTAC 
GAAGCTGACA TGGATATGTT GGCGCGACAG CACAAGCAGC TGGTGGAAAA GACAGAGCAG 
ACGCAGGAGA ACGAGCTGCG CTCCTCATCT AAACGCATTC GCTCCGAGCA GGAGCAAGAG 
TTGAAGATCT TCCGAGAGAA TCTCAAGCAG GAGATCCGAC TGCTCAAGCA GGAAGTTGAC 
CTGTTTCCCA AGGACAAGCG CAAGGATGAG TTCAAGCAGC GGCGCTCAGC CATGGAACTA 
GACCACGAAG AGAAGGAACG CGCCTTCCTG GATTCTTTAA AGGAGAGGCA TGAGCTTCTA 
CTAAGGCGAT TAAGCGAGAA GCATCGGGAC CACCTGGCCA CCATCAACCG CAACTTCTTG 
CAGCAGAAAC AGAACGCCAT GAGGACGCGG GAGGCACTTC TCTGGGAACT AGAGGAGAAG 
CAACTTCACG AACGACATCA GTTGTCCAAG CGGCATGTAA AGGAACTATG CTTCATGCAG 
CGACATCAGA TGATAATCCG GCACGAGAAG GAGCTGGACC AGGTGAAGCG TATGTTGCAG 
CGTAAGGAGG AGGACATGGT CAAGAAACAG ACGATGGAAA AGCGGGCGCT GCCAAAGCGC 
ATCCGTGCGG AACGCAAGGC GCGAGATCTT ATGTTCCGTG AATCCCTTCG AATTTCCACG 
AACCTAGATC CTGAAATCGA ACGCGATCGC TTGAAGAAGT TCCAGGAGCA AGAGAAAAAG 
CGTTATATGC AGGAGGAGCG ACGCTTCGAA GTCAAGCATC AAAAGCAATT GGAAGAGCTA 
CGCGCTACAC GCGAAAGCGC CATAAAAGAG CTGGAGCAAC TTCAGAACGA AAAGCGAAGG 
GCTCTGGTGG AGCACGAACA CTCCAAGCTT TCCGAGATCG ATGAGCGACT GAAGGGGGAG 
CTGCGCGAGT GGCGCGAACA GTTGGTGCCC CGTAAGCAGC GCCTTGAGGA AACCTTCGCC 
CAACAGCTGG ACGAGATGGA GACCCTGTAC GGCGGCGCCC TGATTGTGTC CATGCCCTCG 
GACACGCTGC AGCGAGATCA CTTCACCGGC TCGACGCGCA GCAGCCTCAG CTCGTATTCC 
GAGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slik ( NM_001144280.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137752.1 Drosophila melanogaster Sterile20-like kinase (Slik), transcript variant F, mRNA.

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CloneID	OFa13241
Clone ID Related Accession (Same CDS sequence)	NM_001144280.2
Accession Version	NM_001144280.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Sterile20-like kinase, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001144280.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTTTA TCACCAACCT GAAGAAGGTC TTCCACCTCG GCGGGGGTGA GGCCAAGAAG 
AAGCGCCTGT ACAACAACAT CAAGATGGAC ACGGATCCGG CGGAGTTCTG GGAAATGGTC 
GGCGAACTGG GCGACGGAGC CTTTGGCAAG GTTTACAAGG CTCAGCACAA GGAGCAGAAG 
CGATTTGCTG CCGCAAAAAT GTGTCAGCTG GAGGACGAGG AGAACCTTAG CGATCACATG 
GTCGAGATCG ACATTCTGTC GGAGATCAAG CACCCAAATA TAGTGGAGCT GTATGAGGCC 
TTTTCCATCG ATGATAAGCT GTGGATGCTC ATCGAGTACT GCGACGGAGG GGCCCTGGAT 
AGCATTATGG TGGAGCTGGA AAAGCCGCTG ACTGAGCCGC AGATTGCCTA TGTTTGCAAG 
CACATGACCG AGGGTCTCAC CTTCCTGCAT CGCAACAAAG TCATCCATCG TGATTTGAAG 
GCAGGAAACG TGCTTCTCAC AATGGAAGGT GGCGTCAAGT TGGCGGACTT TGGCGTTTCG 
GCCAAAAACA AGCACACCAT GCAAAAGCAC GACACCTTCA TTGGCACTCC CTACTGGATG 
GCCCCGGAGC TGGTGCTTTG CGAAACTTTC CGGGACAACC CGTACGATCA CAAAGTGGAC 
ATCTGGTCAC TGGGCATCAC GCTCATTGAG CTGGCGCAGA TGGAGCCGCC CAACAGCGAG 
ATGTCGCCCA TGCGAGTGCT CTTGAAAATA CAAAAGAGCG AGCCGCCCAA GCTGGAGCAG 
CCTTCTCGAT GGAGCAAGGA GTTTAACGAC TTCCTGAAGA AGTCCTTAGT CAAAGATCCG 
CAGGTGAGGC CCACGACGGA TGTGCTGATG CAGCACGCTT TCATCAACAG AAATCTGGAT 
GCCAAGCCTA TCAAGGATCT GCTGCTTGAG TACAAGGCCG AGGTCGTGGA AGAGGTGGTG 
GATGATGAGA CCGAGGAACC CCGCAACTCG GCGCTCCAGC TCGACCTGGA CGATGACTCT 
GCTTCGCTGC AGAGCCAAGA CATTGACAAA CTTCCAGGTA CACCTACATC CATTTTGCGG 
GATGCCAAAG AGCAGTCCCA ACCAAGTAGC AGTTTACCAA TCGCAGCAGC GGCAACTGCA 
GCAGCAGCAG CTACAACCAC AACTAAAGCA ACCACTCCGG ACAGACCAAA CCACACAAAG 
GATGACAATG CTGAAGCGGC AGCCCAGCAG CCGCCTCATA CCAAAGTGCC TGCTCCAGCG 
CCGCCGTCGT CCCAACAAAC GCCACCACCG CAAGTTCAGC AGCCACCTAC ACCACCAGCC 
CAACCCACAG CGGCTGTGCT GCAAAAGAAA CCCGAAGATG TGGCAGCTGT TGCAGAAACA 
TCTGAGAAAA CCGAATCCGA CAAGAAGCAC TTTGTCAAAA AGGGTAAGGC CCCGCCCCCG 
CCGTCCCCAT TGGGTCTTGC AAATGCCAAG CCGGCTGCCA GCGACTCCCA GACGTCTCCC 
AAAAAACTGG CCACTCCCGA GCCCACTTCT CCAGTCACCA CGGCTATTGA GGTGGCCATT 
GGCCAAGAAG CGATGGAGCC CAAACCGCAG CCGCCATCGC CCACAGCCTC CTCCATTGTG 
TCTGTGCAAT CGGTGGCCTC TTCCAGCTCT TCGGGCAGTG TTTCAAATGC TGTTCTCAGC 
TCGAGCACTT CCCTTATTAC CATCAACAGC GATCCACCCA CACCGCATCA TCACCAACCG 
CTGCCACCTC AACCGCAGCA CTTGATTTTG CCAAACAGCT TGGAGTCGGT GAGTCAGATA 
ACAGTCGTGA CCAGCACCCA TCCGCCGGTA ATCATCGACA ACTCGGTGAT GCCACCGCAA 
AACGAGGTGA TCATCGTATC CAATGATATG AACAAGAGCA CTCACCTGCA TGAATCTTCG 
ACGGACGATG ATTTTCCATC GCTGGACGAC AGTTTGGGTG ATGCCACCAC GCCGCCCCAC 
AAGCAATCCT CAATGATATT GGCTGTTAAT GAGCCAGCAG GTGTGGTTCC TGCTCCTCCG 
TCGCAACCGC AAACGTCTTC CACTGTTCAC GCACGCAAAC TGGATGAAAG TGAGGTGTTA 
ATCGTCAGTC CGTCCTACGC GGATGATGAC TCGGCCTATA ATACGGCATC TGGCAGTCAT 
AGCCACGATC ACAGTGACCA TCTGATGGAC ACGAGCCACG TCTCCGTGGT CACCGTGGGC 
GATGAGGGAG TTAAGGTGAA GGACAGCAGC AATGAGCTGG TCAAGCGTCA GCCCAATGGA 
GTTGGCATTG TGCCGGAAGA CGTAAACATA ATTGTAAACC GATTCAAACA GGAGAAGCGG 
TCGCCGGACA GCTCGCTGAG CGAGAATGGC TCGGTGCGCG GACGGCGTGG TATTGAGGTG 
CTGGTTGGCG GCAGCGGAGG ATCCGACGTG GACAGCATTG GCACAAACAC TAGTCAGGAA 
AGCCGGCACG AAACGGATCA CAACAACAAG CAGCAGTATC CAGCGGCCGC GCTGATGCCT 
CCACCACCTC CCTCGTTGAC GAATAACCAC AATCACGAGA CCATTGATGA GGAAGAGGAG 
GTGGTGATCC GACCCAAGGC TAGGGTTCCG GCCGTAGTTA AGTCTGCAAA TGCTCAAGGT 
CTTACCAAGG AAGAGATTGA GCTGCGCAAT CTGCGCAAGA AGACACGCAA GCGCACCCGC 
AAATTCGAGA TCGATGGCGT GCAAATGACC ACCACCACAA GCCGAGTGAT CTACGGGGAC 
GATGAGAACG GACGAATCTA CGATGATCAC GACTTCCGTA AGCAGGAGCT ACGTGAATTA 
AAGCTGCTGC AGAAGCAGGA GAAGAAACAG CAGACGGAAC TGCACTTAAA GGAGCAGCAG 
GCCAAGGAAC AGCAGGATCG TCGCTTTGAG CAGGAGCGCT CCTCGCTGGA AAAGACCTAC 
GAAGCTGACA TGGATATGTT GGCGCGACAG CACAAGCAGC TGGTGGAAAA GACAGAGCAG 
ACGCAGGAGA ACGAGCTGCG CTCCTCATCT AAACGCATTC GCTCCGAGCA GGAGCAAGAG 
TTGAAGATCT TCCGAGAGAA TCTCAAGCAG GAGATCCGAC TGCTCAAGCA GGAAGTTGAC 
CTGTTTCCCA AGGACAAGCG CAAGGATGAG TTCAAGCAGC GGCGCTCAGC CATGGAACTA 
GACCACGAAG AGAAGGAACG CGCCTTCCTG GATTCTTTAA AGGAGAGGCA TGAGCTTCTA 
CTAAGGCGAT TAAGCGAGAA GCATCGGGAC CACCTGGCCA CCATCAACCG CAACTTCTTG 
CAGCAGAAAC AGAACGCCAT GAGGACGCGG GAGGCACTTC TCTGGGAACT AGAGGAGAAG 
CAACTTCACG AACGACATCA GTTGTCCAAG CGGCATGTAA AGGAACTATG CTTCATGCAG 
CGACATCAGA TGATAATCCG GCACGAGAAG GAGCTGGACC AGGTGAAGCG TATGTTGCAG 
CGTAAGGAGG AGGACATGGT CAAGAAACAG ACGATGGAAA AGCGGGCGCT GCCAAAGCGC 
ATCCGTGCGG AACGCAAGGC GCGAGATCTT ATGTTCCGTG AATCCCTTCG AATTTCCACG 
AACCTAGATC CTGAAATCGA ACGCGATCGC TTGAAGAAGT TCCAGGAGCA AGAGAAAAAG 
CGTTATATGC AGGAGGAGCG ACGCTTCGAA GTCAAGCATC AAAAGCAATT GGAAGAGCTA 
CGCGCTACAC GCGAAAGCGC CATAAAAGAG CTGGAGCAAC TTCAGAACGA AAAGCGAAGG 
GCTCTGGTGG AGCACGAACA CTCCAAGCTT TCCGAGATCG ATGAGCGACT GAAGGGGGAG 
CTGCGCGAGT GGCGCGAACA GTTGGTGCCC CGTAAGCAGG AGCTGAATCG ACAGATCAAA 
CTGGCCATTG ATCAGCACGA AAAGCGTTTT GGTCTTGTGA CCAATCGCGA GGAGTTCGAG 
GATCAGGAGG TCAAGCTGCC CGCCCACTTG CGAAACATTT ACAACGAGCG ATCCTCACGC 
ATTCTGCCCC GCAACACCTT TATCGACCCT CAAAACATGC CGCGCTCTCG CTCCTCGCTC 
CTAATGATGG GGGGTGGTGG CAACCGACTG TCCAACTTCC GCGGATCAGC TCCAGATCTT 
AGCCGCAGTG TGCCCAACAC TCCTATTATG CACAAACAAC AGAGATCGCA GATGATAGGC 
GATCAGGTGC TAGAAGAGGA CGAGGAGCAG ATGCTGAGCG AGCAGATGAA GCGGGCCAGC 
GTCGCCACGC TTCCGGCGCA AAACCAACTT CGCCTTATTA CTCAGCTTCC GGCAAACGAG 
CTCGTGAAGG TAGTGACCTC CACTCCACCA CTTAACAGCG CGGAGGATGT GAACTCCAAG 
CCATCCATGA GCACATTCAG AGGCAGTACG CCGCCCAAAA CACCAGATGA TCTTGGCTTG 
GTCACCAATC GATTCAGCCG CCTGGACACC AAAGCTGCAG CGGAGGCCAG CGGCGTGGAG 
TTGCGCATTC ACGAGGGACC AGTGCTAACC GCTCACACTC AGCGGCTGCT GCACACCACC 
TCCTTTGCTA TCAAGCGACC CTCACTGGCC AGCCGGAGCA GCGGCAGATC CTCCCTGAGC 
AGTGCGCAGT CCATGTACAA CATAAATGAG CTGTCCACGC GGTTCACCAG TGATGCGGAC 
CTGATCTTTG ACTCCGGACG CAAGGCTGCC GCCCTGCTAG ACGAAGTGCA ACCGCAGCTA 
CGGTCCTCCG TTAAGCCAGG ACATAGTCGC GCCAATGGAA GCGTCGTCTC AGCCGACTCG 
GCCGCCTCTT CCGAGGATAT CTACTACGAC CTGTATGCGG CGCAGTACAA ACTGCCGAGA 
GTCAATCAGC GGCAGCACAG CTGCGAGGAG GACTCGTCGG CAATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137752.1
CDS	342..5249
Translation	MSFITNLKKVFHLGGGEAKKKRLYNNIKMDTDPAEFWEMVGELGDGAFGKVYKAQHKEQKRFAAAKMCQLEDEENLSDHMVEIDILSEIKHPNIVELYEAFSIDDKLWMLIEYCDGGALDSIMVELEKPLTEPQIAYVCKHMTEGLTFLHRNKVIHRDLKAGNVLLTMEGGVKLADFGVSAKNKHTMQKHDTFIGTPYWMAPELVLCETFRDNPYDHKVDIWSLGITLIELAQMEPPNSEMSPMRVLLKIQKSEPPKLEQPSRWSKEFNDFLKKSLVKDPQVRPTTDVLMQHAFINRNLDAKPIKDLLLEYKAEVVEEVVDDETEEPRNSALQLDLDDDSASLQSQDIDKLPGTPTSILRDAKEQSQPSSSLPIAAAATAAAAATTTTKATTPDRPNHTKDDNAEAAAQQPPHTKVPAPAPPSSQQTPPPQVQQPPTPPAQPTAAVLQKKPEDVAAVAETSEKTESDKKHFVKKGKAPPPPSPLGLANAKPAASDSQTSPKKLATPEPTSPVTTAIEVAIGQEAMEPKPQPPSPTASSIVSVQSVASSSSSGSVSNAVLSSSTSLITINSDPPTPHHHQPLPPQPQHLILPNSLESVSQITVVTSTHPPVIIDNSVMPPQNEVIIVSNDMNKSTHLHESSTDDDFPSLDDSLGDATTPPHKQSSMILAVNEPAGVVPAPPSQPQTSSTVHARKLDESEVLIVSPSYADDDSAYNTASGSHSHDHSDHLMDTSHVSVVTVGDEGVKVKDSSNELVKRQPNGVGIVPEDVNIIVNRFKQEKRSPDSSLSENGSVRGRRGIEVLVGGSGGSDVDSIGTNTSQESRHETDHNNKQQYPAAALMPPPPPSLTNNHNHETIDEEEEVVIRPKARVPAVVKSANAQGLTKEEIELRNLRKKTRKRTRKFEIDGVQMTTTTSRVIYGDDENGRIYDDHDFRKQELRELKLLQKQEKKQQTELHLKEQQAKEQQDRRFEQERSSLEKTYEADMDMLARQHKQLVEKTEQTQENELRSSSKRIRSEQEQELKIFRENLKQEIRLLKQEVDLFPKDKRKDEFKQRRSAMELDHEEKERAFLDSLKERHELLLRRLSEKHRDHLATINRNFLQQKQNAMRTREALLWELEEKQLHERHQLSKRHVKELCFMQRHQMIIRHEKELDQVKRMLQRKEEDMVKKQTMEKRALPKRIRAERKARDLMFRESLRISTNLDPEIERDRLKKFQEQEKKRYMQEERRFEVKHQKQLEELRATRESAIKELEQLQNEKRRALVEHEHSKLSEIDERLKGELREWREQLVPRKQELNRQIKLAIDQHEKRFGLVTNREEFEDQEVKLPAHLRNIYNERSSRILPRNTFIDPQNMPRSRSSLLMMGGGGNRLSNFRGSAPDLSRSVPNTPIMHKQQRSQMIGDQVLEEDEEQMLSEQMKRASVATLPAQNQLRLITQLPANELVKVVTSTPPLNSAEDVNSKPSMSTFRGSTPPKTPDDLGLVTNRFSRLDTKAAAEASGVELRIHEGPVLTAHTQRLLHTTSFAIKRPSLASRSSGRSSLSSAQSMYNINELSTRFTSDADLIFDSGRKAAALLDEVQPQLRSSVKPGHSRANGSVVSADSAASSEDIYYDLYAAQYKLPRVNQRQHSCEEDSSAI

Target ORF information:

RefSeq Version	NM_001144280.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Sterile20-like kinase (Slik), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144280.2

ORF Insert Sequence:

ATGTCGTTTA TCACCAACCT GAAGAAGGTC TTCCACCTCG GCGGGGGTGA GGCCAAGAAG 
AAGCGCCTGT ACAACAACAT CAAGATGGAC ACGGATCCGG CGGAGTTCTG GGAAATGGTC 
GGCGAACTGG GCGACGGAGC CTTTGGCAAG GTTTACAAGG CTCAGCACAA GGAGCAGAAG 
CGATTTGCTG CCGCAAAAAT GTGTCAGCTG GAGGACGAGG AGAACCTTAG CGATCACATG 
GTCGAGATCG ACATTCTGTC GGAGATCAAG CACCCAAATA TAGTGGAGCT GTATGAGGCC 
TTTTCCATCG ATGATAAGCT GTGGATGCTC ATCGAGTACT GCGACGGAGG GGCCCTGGAT 
AGCATTATGG TGGAGCTGGA AAAGCCGCTG ACTGAGCCGC AGATTGCCTA TGTTTGCAAG 
CACATGACCG AGGGTCTCAC CTTCCTGCAT CGCAACAAAG TCATCCATCG TGATTTGAAG 
GCAGGAAACG TGCTTCTCAC AATGGAAGGT GGCGTCAAGT TGGCGGACTT TGGCGTTTCG 
GCCAAAAACA AGCACACCAT GCAAAAGCAC GACACCTTCA TTGGCACTCC CTACTGGATG 
GCCCCGGAGC TGGTGCTTTG CGAAACTTTC CGGGACAACC CGTACGATCA CAAAGTGGAC 
ATCTGGTCAC TGGGCATCAC GCTCATTGAG CTGGCGCAGA TGGAGCCGCC CAACAGCGAG 
ATGTCGCCCA TGCGAGTGCT CTTGAAAATA CAAAAGAGCG AGCCGCCCAA GCTGGAGCAG 
CCTTCTCGAT GGAGCAAGGA GTTTAACGAC TTCCTGAAGA AGTCCTTAGT CAAAGATCCG 
CAGGTGAGGC CCACGACGGA TGTGCTGATG CAGCACGCTT TCATCAACAG AAATCTGGAT 
GCCAAGCCTA TCAAGGATCT GCTGCTTGAG TACAAGGCCG AGGTCGTGGA AGAGGTGGTG 
GATGATGAGA CCGAGGAACC CCGCAACTCG GCGCTCCAGC TCGACCTGGA CGATGACTCT 
GCTTCGCTGC AGAGCCAAGA CATTGACAAA CTTCCAGGTA CACCTACATC CATTTTGCGG 
GATGCCAAAG AGCAGTCCCA ACCAAGTAGC AGTTTACCAA TCGCAGCAGC GGCAACTGCA 
GCAGCAGCAG CTACAACCAC AACTAAAGCA ACCACTCCGG ACAGACCAAA CCACACAAAG 
GATGACAATG CTGAAGCGGC AGCCCAGCAG CCGCCTCATA CCAAAGTGCC TGCTCCAGCG 
CCGCCGTCGT CCCAACAAAC GCCACCACCG CAAGTTCAGC AGCCACCTAC ACCACCAGCC 
CAACCCACAG CGGCTGTGCT GCAAAAGAAA CCCGAAGATG TGGCAGCTGT TGCAGAAACA 
TCTGAGAAAA CCGAATCCGA CAAGAAGCAC TTTGTCAAAA AGGGTAAGGC CCCGCCCCCG 
CCGTCCCCAT TGGGTCTTGC AAATGCCAAG CCGGCTGCCA GCGACTCCCA GACGTCTCCC 
AAAAAACTGG CCACTCCCGA GCCCACTTCT CCAGTCACCA CGGCTATTGA GGTGGCCATT 
GGCCAAGAAG CGATGGAGCC CAAACCGCAG CCGCCATCGC CCACAGCCTC CTCCATTGTG 
TCTGTGCAAT CGGTGGCCTC TTCCAGCTCT TCGGGCAGTG TTTCAAATGC TGTTCTCAGC 
TCGAGCACTT CCCTTATTAC CATCAACAGC GATCCACCCA CACCGCATCA TCACCAACCG 
CTGCCACCTC AACCGCAGCA CTTGATTTTG CCAAACAGCT TGGAGTCGGT GAGTCAGATA 
ACAGTCGTGA CCAGCACCCA TCCGCCGGTA ATCATCGACA ACTCGGTGAT GCCACCGCAA 
AACGAGGTGA TCATCGTATC CAATGATATG AACAAGAGCA CTCACCTGCA TGAATCTTCG 
ACGGACGATG ATTTTCCATC GCTGGACGAC AGTTTGGGTG ATGCCACCAC GCCGCCCCAC 
AAGCAATCCT CAATGATATT GGCTGTTAAT GAGCCAGCAG GTGTGGTTCC TGCTCCTCCG 
TCGCAACCGC AAACGTCTTC CACTGTTCAC GCACGCAAAC TGGATGAAAG TGAGGTGTTA 
ATCGTCAGTC CGTCCTACGC GGATGATGAC TCGGCCTATA ATACGGCATC TGGCAGTCAT 
AGCCACGATC ACAGTGACCA TCTGATGGAC ACGAGCCACG TCTCCGTGGT CACCGTGGGC 
GATGAGGGAG TTAAGGTGAA GGACAGCAGC AATGAGCTGG TCAAGCGTCA GCCCAATGGA 
GTTGGCATTG TGCCGGAAGA CGTAAACATA ATTGTAAACC GATTCAAACA GGAGAAGCGG 
TCGCCGGACA GCTCGCTGAG CGAGAATGGC TCGGTGCGCG GACGGCGTGG TATTGAGGTG 
CTGGTTGGCG GCAGCGGAGG ATCCGACGTG GACAGCATTG GCACAAACAC TAGTCAGGAA 
AGCCGGCACG AAACGGATCA CAACAACAAG CAGCAGTATC CAGCGGCCGC GCTGATGCCT 
CCACCACCTC CCTCGTTGAC GAATAACCAC AATCACGAGA CCATTGATGA GGAAGAGGAG 
GTGGTGATCC GACCCAAGGC TAGGGTTCCG GCCGTAGTTA AGTCTGCAAA TGCTCAAGGT 
CTTACCAAGG AAGAGATTGA GCTGCGCAAT CTGCGCAAGA AGACACGCAA GCGCACCCGC 
AAATTCGAGA TCGATGGCGT GCAAATGACC ACCACCACAA GCCGAGTGAT CTACGGGGAC 
GATGAGAACG GACGAATCTA CGATGATCAC GACTTCCGTA AGCAGGAGCT ACGTGAATTA 
AAGCTGCTGC AGAAGCAGGA GAAGAAACAG CAGACGGAAC TGCACTTAAA GGAGCAGCAG 
GCCAAGGAAC AGCAGGATCG TCGCTTTGAG CAGGAGCGCT CCTCGCTGGA AAAGACCTAC 
GAAGCTGACA TGGATATGTT GGCGCGACAG CACAAGCAGC TGGTGGAAAA GACAGAGCAG 
ACGCAGGAGA ACGAGCTGCG CTCCTCATCT AAACGCATTC GCTCCGAGCA GGAGCAAGAG 
TTGAAGATCT TCCGAGAGAA TCTCAAGCAG GAGATCCGAC TGCTCAAGCA GGAAGTTGAC 
CTGTTTCCCA AGGACAAGCG CAAGGATGAG TTCAAGCAGC GGCGCTCAGC CATGGAACTA 
GACCACGAAG AGAAGGAACG CGCCTTCCTG GATTCTTTAA AGGAGAGGCA TGAGCTTCTA 
CTAAGGCGAT TAAGCGAGAA GCATCGGGAC CACCTGGCCA CCATCAACCG CAACTTCTTG 
CAGCAGAAAC AGAACGCCAT GAGGACGCGG GAGGCACTTC TCTGGGAACT AGAGGAGAAG 
CAACTTCACG AACGACATCA GTTGTCCAAG CGGCATGTAA AGGAACTATG CTTCATGCAG 
CGACATCAGA TGATAATCCG GCACGAGAAG GAGCTGGACC AGGTGAAGCG TATGTTGCAG 
CGTAAGGAGG AGGACATGGT CAAGAAACAG ACGATGGAAA AGCGGGCGCT GCCAAAGCGC 
ATCCGTGCGG AACGCAAGGC GCGAGATCTT ATGTTCCGTG AATCCCTTCG AATTTCCACG 
AACCTAGATC CTGAAATCGA ACGCGATCGC TTGAAGAAGT TCCAGGAGCA AGAGAAAAAG 
CGTTATATGC AGGAGGAGCG ACGCTTCGAA GTCAAGCATC AAAAGCAATT GGAAGAGCTA 
CGCGCTACAC GCGAAAGCGC CATAAAAGAG CTGGAGCAAC TTCAGAACGA AAAGCGAAGG 
GCTCTGGTGG AGCACGAACA CTCCAAGCTT TCCGAGATCG ATGAGCGACT GAAGGGGGAG 
CTGCGCGAGT GGCGCGAACA GTTGGTGCCC CGTAAGCAGG AGCTGAATCG ACAGATCAAA 
CTGGCCATTG ATCAGCACGA AAAGCGTTTT GGTCTTGTGA CCAATCGCGA GGAGTTCGAG 
GATCAGGAGG TCAAGCTGCC CGCCCACTTG CGAAACATTT ACAACGAGCG ATCCTCACGC 
ATTCTGCCCC GCAACACCTT TATCGACCCT CAAAACATGC CGCGCTCTCG CTCCTCGCTC 
CTAATGATGG GGGGTGGTGG CAACCGACTG TCCAACTTCC GCGGATCAGC TCCAGATCTT 
AGCCGCAGTG TGCCCAACAC TCCTATTATG CACAAACAAC AGAGATCGCA GATGATAGGC 
GATCAGGTGC TAGAAGAGGA CGAGGAGCAG ATGCTGAGCG AGCAGATGAA GCGGGCCAGC 
GTCGCCACGC TTCCGGCGCA AAACCAACTT CGCCTTATTA CTCAGCTTCC GGCAAACGAG 
CTCGTGAAGG TAGTGACCTC CACTCCACCA CTTAACAGCG CGGAGGATGT GAACTCCAAG 
CCATCCATGA GCACATTCAG AGGCAGTACG CCGCCCAAAA CACCAGATGA TCTTGGCTTG 
GTCACCAATC GATTCAGCCG CCTGGACACC AAAGCTGCAG CGGAGGCCAG CGGCGTGGAG 
TTGCGCATTC ACGAGGGACC AGTGCTAACC GCTCACACTC AGCGGCTGCT GCACACCACC 
TCCTTTGCTA TCAAGCGACC CTCACTGGCC AGCCGGAGCA GCGGCAGATC CTCCCTGAGC 
AGTGCGCAGT CCATGTACAA CATAAATGAG CTGTCCACGC GGTTCACCAG TGATGCGGAC 
CTGATCTTTG ACTCCGGACG CAAGGCTGCC GCCCTGCTAG ACGAAGTGCA ACCGCAGCTA 
CGGTCCTCCG TTAAGCCAGG ACATAGTCGC GCCAATGGAA GCGTCGTCTC AGCCGACTCG 
GCCGCCTCTT CCGAGGATAT CTACTACGAC CTGTATGCGG CGCAGTACAA ACTGCCGAGA 
GTCAATCAGC GGCAGCACAG CTGCGAGGAG GACTCGTCGG CAATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Slik cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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