mAChR-A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	mAChR-A
Entrez Gene ID	37892
Full Name	muscarinic Acetylcholine Receptor, A-type
Synonyms	Acr60C,AcrC,CG4356,DM1,Dm-mAChR-A,Dm1,Dmel\CG4356,mAChR,mAChR A,mAChR-1,mAcR,mAcR-60C,mAcR60C
General protein information	Preferred Names muscarinic Acetylcholine Receptor, A-type Names CG4356 gene product from transcript CG4356-RA A-type mAChR CG4356-PA CG4356-PB mAChR-A-PA mAChR-A-PB muscarinic acetylcholine Receptor, A-type muscarinic acetylcholine receptor muscarinic acetylcholine receptor 60C muscarinic receptor
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 60C7-60C7

mRNA and Protein(s)

mRNA	Protein	Name
NM_166668.2	NP_726440.1	muscarinic acetylcholine Receptor, A-type, isoform A
NM_079120.3	NP_523844.2	muscarinic acetylcholine Receptor, A-type, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	CHRM1	NP_000729.2
Pan troglodytes (chimpanzee)	CHRM1	XP_508508.2
Mus musculus (house mouse)	Chrm1	NP_001106167.1
Rattus norvegicus (Norway rat)	Chrm1	NP_542951.1
Canis lupus familiaris (dog)	CHRM1	XP_005631654.1
Caenorhabditis elegans (roundworm)	gar-3	NP_001024236.1
Xenopus tropicalis (tropical clawed frog)	LOC100490434	XP_004913718.1
	CHRM1	NP_001028117.1
Bos taurus (cattle)	CHRM1	NP_001231538.1
Drosophila melanogaster (fruit fly)	mAcR-60C	NP_726440.1
Anopheles gambiae (African malaria mosquito)	GPRMAC1	XP_314486.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mAChR-A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mAChR-A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11159	NM_166668.2 Latest version!	Drosophila melanogaster muscarinic acetylcholine Receptor, A-type, transcript variant A (mAChR-A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa11160	NM_079120.3 Latest version!	Drosophila melanogaster muscarinic acetylcholine Receptor, A-type, transcript variant B (mAChR-A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mAChR-A ( NM_166668.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726440.1 Drosophila melanogaster muscarinic acetylcholine Receptor, A-type, transcript variant A (mAChR-A), mRNA.

Price & Availability↑

CloneID	OFa11159
Clone ID Related Accession (Same CDS sequence)	NM_166668.2
Accession Version	NM_166668.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	muscarinic acetylcholine Receptor, A-type, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166668.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCCGG TCATGAGTCT GGCATTGGCC GCCCATGGAC CGCCTAGCAT ACTAGAGCCG 
CTGTTTAAGA CCGTAACCAC GAGCACAACG ACCACCACCA CCACGACGAC CAGCACGACG 
ACGACGACGG CGAGTCCTGC AGGGTATTCG CCAGGATACC CGGGCACCAC TCTTCTGACC 
GCCCTGTTCG AGAACCTGAC GTCCACGGCG GCGAGCGGGC TATACGATCC CTATTCGGGA 
ATGTACGGAA ACCAGACGAA CGGCACCATC GGCTTCGAGA CAAAGGGACC TCGGTACTCA 
CTGGCCTCCA TGGTGGTCAT GGGCTTCGTG GCGGCGATTT TGAGCACGGT GACGGTGGCG 
GGCAATGTCA TGGTGATGAT ATCCTTTAAG ATCGACAAGC AGCTGCAGAC GATCAGCAAC 
TACTTCTTGT TCTCGCTGGC CATCGCGGAC TTCGCCATTG GCGCTATATC GATGCCGCTT 
TTTGCGGTGA CCACAATCCT GGGCTACTGG CCCTTGGGTC CCATCGTCTG CGACACCTGG 
CTGGCCCTGG ACTACCTGGC ATCGAATGCT TCAGTGCTGA ACCTGCTGAT CATCAGTTTC 
GATCGGTACT TCAGTGTCAC TCGACCGCTG ACGTACCGCG CCAAAAGGAC CACCAACCGG 
GCGGCGGTGA TGATTGGCGC CGCCTGGGGC ATCAGCCTGC TCCTCTGGCC GCCCTGGATC 
TACAGCTGGC CCTACATCGA GGGCAAGCGG ACGGTGCCCA AGGACGAGTG CTACATCCAG 
TTCATCGAGA CCAACCAGTA CATCACCTTC GGCACGGCAC TGGCCGCCTT CTACTTCCCG 
GTCACCATCA TGTGCTTCCT CTACTGGCGC ATCTGGCGCG AGACGAAAAA GCGGCAGAAG 
GACCTGCCCA ACCTGCAGGC GGGCAAGAAA GACTCCAGCA AGAGATCAAA CAGCAGTGAC 
GAAAACACGG TGGTGAATCA CGCCTCTGGC GGCCTGCTGG CCTTCGCCCA GGTGGGCGGA 
AACGACCATG ACACCTGGCG ACGTCCGCGC TCCGAGAGTT CACCGGACGC GGAGAGTGTC 
TACATGACCA ACATGGTCAT CGACTCCGGC TACCACGGGA TGCACTCGCG CAAGTCAAGC 
ATCAAAAGCA CGAATACAAT CAAAAAATCG TACACCTGCT TCGGCAGCAT CAAGGAGTGG 
TGCATTGCGT GGTGGCACTC CGGTCGCGAG GACTCCGACG ACTTCGCCTA CGAGCAGGAG 
GAGCCTTCTG ATTTAGGATG CACCTCGATG AACGTGATGC GGGACAACTA CTCGATGGGC 
GGCAGCGTGA GCGGCGTGCG TCCGCCCAGC ATTCTCCTGT CGGATGTCTC GCCCACGCCG 
CTGCCACGCC CACCGCTTGC CTCCATCTCG CAGCTGCAGG AAATGAGTGC GGTTACGGCG 
AGCACAACGG CGAATGTGAA TACCAGTGGC AATGGCAATG GCGCCATCAA CAACAACAAC 
AACGCCAGCC ACAACGGGAA CGGCGCAGTG AACGGGAATG GGGCCGGAAA CGGAAGTGGA 
ATCGGACTGG GTACAACCGG AAATGCGACG CACCGCGACT CGCGCACTCT GCCCGTTATC 
AATCGCATTA ACTCACGGTC GGTGAGCCAG GACTCCGTGT ACACGATACT CATCCGCCTG 
CCTTCCGACG GCGCATCCAG CAACGCGGCC AACGGAGGAG GGGGTGGCCC TGGTGCCGGG 
GCAGCTGCCT CCGCGTCCTT GAGCATGCAA GGCGACTGTG CGCCCTCCAT CAAGATGATA 
CACGAGGACG GGCCCACGAC GACGGCAGCG GCAGCTCCTT TGGCAAGCGC CGCAGCCACA 
CGACGACCTT TGCCCTCGCG AGACTCTGAG TTCAGCCTGC CACTCGGACG GAGGATGTCC 
CATGCCCAGC ACGATGCCAG GCTACTCAAC GCCAAGGTCA TACCCAAGCA GCTGGGCAAG 
GCTGGGGGCG GGGCAGCGGG CGGCGGAGTG GGCGGAGCCC ACGCCCTCAT GAACGCCCGC 
AATGCTGCCA AGAAGAAAAA GAAATCGCAA GAGAAGCGGC AGGAGTCGAA GGCGGCCAAG 
ACGCTGTCGG CTATCCTGCT GAGCTTCATC ATCACGTGGA CTCCGTACAA CATCCTGGTG 
CTGATCAAGC CGCTGACCAC CTGCTCCGAC TGCATACCCA CGGAGCTGTG GGACTTTTTC 
TACGCCCTGT GCTACATCAA CTCGACGATC AACCCCATGT GCTACGCCTT GTGCAACGCC 
ACCTTCCGGA GAACCTACGT CCGGATCCTC ACCTGCAAGT GGCACACCCG GAACCGCGAG 
GGCATGGTAC GCGGCGTCTA CAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726440.1
CDS	867..3233
Translation	MEPVMSLALAAHGPPSILEPLFKTVTTSTTTTTTTTTSTTTTTASPAGYSPGYPGTTLLTALFENLTSTAASGLYDPYSGMYGNQTNGTIGFETKGPRYSLASMVVMGFVAAILSTVTVAGNVMVMISFKIDKQLQTISNYFLFSLAIADFAIGAISMPLFAVTTILGYWPLGPIVCDTWLALDYLASNASVLNLLIISFDRYFSVTRPLTYRAKRTTNRAAVMIGAAWGISLLLWPPWIYSWPYIEGKRTVPKDECYIQFIETNQYITFGTALAAFYFPVTIMCFLYWRIWRETKKRQKDLPNLQAGKKDSSKRSNSSDENTVVNHASGGLLAFAQVGGNDHDTWRRPRSESSPDAESVYMTNMVIDSGYHGMHSRKSSIKSTNTIKKSYTCFGSIKEWCIAWWHSGREDSDDFAYEQEEPSDLGCTSMNVMRDNYSMGGSVSGVRPPSILLSDVSPTPLPRPPLASISQLQEMSAVTASTTANVNTSGNGNGAINNNNNASHNGNGAVNGNGAGNGSGIGLGTTGNATHRDSRTLPVINRINSRSVSQDSVYTILIRLPSDGASSNAANGGGGGPGAGAAASASLSMQGDCAPSIKMIHEDGPTTTAAAAPLASAAATRRPLPSRDSEFSLPLGRRMSHAQHDARLLNAKVIPKQLGKAGGGAAGGGVGGAHALMNARNAAKKKKKSQEKRQESKAAKTLSAILLSFIITWTPYNILVLIKPLTTCSDCIPTELWDFFYALCYINSTINPMCYALCNATFRRTYVRILTCKWHTRNREGMVRGVYN

Target ORF information:

RefSeq Version	NM_166668.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster muscarinic acetylcholine Receptor, A-type, transcript variant A (mAChR-A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166668.2

ORF Insert Sequence:

ATGGAGCCGG TCATGAGTCT GGCATTGGCC GCCCATGGAC CGCCTAGCAT ACTAGAGCCG 
CTGTTTAAGA CCGTAACCAC GAGCACAACG ACCACCACCA CCACGACGAC CAGCACGACG 
ACGACGACGG CGAGTCCTGC AGGGTATTCG CCAGGATACC CGGGCACCAC TCTTCTGACC 
GCCCTGTTCG AGAACCTGAC GTCCACGGCG GCGAGCGGGC TATACGATCC CTATTCGGGA 
ATGTACGGAA ACCAGACGAA CGGCACCATC GGCTTCGAGA CAAAGGGACC TCGGTACTCA 
CTGGCCTCCA TGGTGGTCAT GGGCTTCGTG GCGGCGATTT TGAGCACGGT GACGGTGGCG 
GGCAATGTCA TGGTGATGAT ATCCTTTAAG ATCGACAAGC AGCTGCAGAC GATCAGCAAC 
TACTTCTTGT TCTCGCTGGC CATCGCGGAC TTCGCCATTG GCGCTATATC GATGCCGCTT 
TTTGCGGTGA CCACAATCCT GGGCTACTGG CCCTTGGGTC CCATCGTCTG CGACACCTGG 
CTGGCCCTGG ACTACCTGGC ATCGAATGCT TCAGTGCTGA ACCTGCTGAT CATCAGTTTC 
GATCGGTACT TCAGTGTCAC TCGACCGCTG ACGTACCGCG CCAAAAGGAC CACCAACCGG 
GCGGCGGTGA TGATTGGCGC CGCCTGGGGC ATCAGCCTGC TCCTCTGGCC GCCCTGGATC 
TACAGCTGGC CCTACATCGA GGGCAAGCGG ACGGTGCCCA AGGACGAGTG CTACATCCAG 
TTCATCGAGA CCAACCAGTA CATCACCTTC GGCACGGCAC TGGCCGCCTT CTACTTCCCG 
GTCACCATCA TGTGCTTCCT CTACTGGCGC ATCTGGCGCG AGACGAAAAA GCGGCAGAAG 
GACCTGCCCA ACCTGCAGGC GGGCAAGAAA GACTCCAGCA AGAGATCAAA CAGCAGTGAC 
GAAAACACGG TGGTGAATCA CGCCTCTGGC GGCCTGCTGG CCTTCGCCCA GGTGGGCGGA 
AACGACCATG ACACCTGGCG ACGTCCGCGC TCCGAGAGTT CACCGGACGC GGAGAGTGTC 
TACATGACCA ACATGGTCAT CGACTCCGGC TACCACGGGA TGCACTCGCG CAAGTCAAGC 
ATCAAAAGCA CGAATACAAT CAAAAAATCG TACACCTGCT TCGGCAGCAT CAAGGAGTGG 
TGCATTGCGT GGTGGCACTC CGGTCGCGAG GACTCCGACG ACTTCGCCTA CGAGCAGGAG 
GAGCCTTCTG ATTTAGGATG CACCTCGATG AACGTGATGC GGGACAACTA CTCGATGGGC 
GGCAGCGTGA GCGGCGTGCG TCCGCCCAGC ATTCTCCTGT CGGATGTCTC GCCCACGCCG 
CTGCCACGCC CACCGCTTGC CTCCATCTCG CAGCTGCAGG AAATGAGTGC GGTTACGGCG 
AGCACAACGG CGAATGTGAA TACCAGTGGC AATGGCAATG GCGCCATCAA CAACAACAAC 
AACGCCAGCC ACAACGGGAA CGGCGCAGTG AACGGGAATG GGGCCGGAAA CGGAAGTGGA 
ATCGGACTGG GTACAACCGG AAATGCGACG CACCGCGACT CGCGCACTCT GCCCGTTATC 
AATCGCATTA ACTCACGGTC GGTGAGCCAG GACTCCGTGT ACACGATACT CATCCGCCTG 
CCTTCCGACG GCGCATCCAG CAACGCGGCC AACGGAGGAG GGGGTGGCCC TGGTGCCGGG 
GCAGCTGCCT CCGCGTCCTT GAGCATGCAA GGCGACTGTG CGCCCTCCAT CAAGATGATA 
CACGAGGACG GGCCCACGAC GACGGCAGCG GCAGCTCCTT TGGCAAGCGC CGCAGCCACA 
CGACGACCTT TGCCCTCGCG AGACTCTGAG TTCAGCCTGC CACTCGGACG GAGGATGTCC 
CATGCCCAGC ACGATGCCAG GCTACTCAAC GCCAAGGTCA TACCCAAGCA GCTGGGCAAG 
GCTGGGGGCG GGGCAGCGGG CGGCGGAGTG GGCGGAGCCC ACGCCCTCAT GAACGCCCGC 
AATGCTGCCA AGAAGAAAAA GAAATCGCAA GAGAAGCGGC AGGAGTCGAA GGCGGCCAAG 
ACGCTGTCGG CTATCCTGCT GAGCTTCATC ATCACGTGGA CTCCGTACAA CATCCTGGTG 
CTGATCAAGC CGCTGACCAC CTGCTCCGAC TGCATACCCA CGGAGCTGTG GGACTTTTTC 
TACGCCCTGT GCTACATCAA CTCGACGATC AACCCCATGT GCTACGCCTT GTGCAACGCC 
ACCTTCCGGA GAACCTACGT CCGGATCCTC ACCTGCAAGT GGCACACCCG GAACCGCGAG 
GGCATGGTAC GCGGCGTCTA CAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mAChR-A ( NM_079120.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523844.2 Drosophila melanogaster muscarinic acetylcholine Receptor, A-type, transcript variant B (mAChR-A), mRNA.

Price & Availability↑

CloneID	OFa11160
Clone ID Related Accession (Same CDS sequence)	NM_079120.3
Accession Version	NM_079120.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	muscarinic acetylcholine Receptor, A-type, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_079120.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCCGG TCATGAGTCT GGCATTGGCC GCCCATGGAC CGCCTAGCAT ACTAGAGCCG 
CTGTTTAAGA CCGTAACCAC GAGCACAACG ACCACCACCA CCACGACGAC CAGCACGACG 
ACGACGACGG CGAGTCCTGC AGGGTATTCG CCAGGATACC CGGGCACCAC TCTTCTGACC 
GCCCTGTTCG AGAACCTGAC GTCCACGGCG GCGAGCGGGC TATACGATCC CTATTCGGGA 
ATGTACGGAA ACCAGACGAA CGGCACCATC GGCTTCGAGA CAAAGGGACC TCGGTACTCA 
CTGGCCTCCA TGGTGGTCAT GGGCTTCGTG GCGGCGATTT TGAGCACGGT GACGGTGGCG 
GGCAATGTCA TGGTGATGAT ATCCTTTAAG ATCGACAAGC AGCTGCAGAC GATCAGCAAC 
TACTTCTTGT TCTCGCTGGC CATCGCGGAC TTCGCCATTG GCGCTATATC GATGCCGCTT 
TTTGCGGTGA CCACAATCCT GGGCTACTGG CCCTTGGGTC CCATCGTCTG CGACACCTGG 
CTGGCCCTGG ACTACCTGGC ATCGAATGCT TCAGTGCTGA ACCTGCTGAT CATCAGTTTC 
GATCGGTACT TCAGTGTCAC TCGACCGCTG ACGTACCGCG CCAAAAGGAC CACCAACCGG 
GCGGCGGTGA TGATTGGCGC CGCCTGGGGC ATCAGCCTGC TCCTCTGGCC GCCCTGGATC 
TACAGCTGGC CCTACATCGA GGGCAAGCGG ACGGTGCCCA AGGACGAGTG CTACATCCAG 
TTCATCGAGA CCAACCAGTA CATCACCTTC GGCACGGCAC TGGCCGCCTT CTACTTCCCG 
GTCACCATCA TGTGCTTCCT CTACTGGCGC ATCTGGCGCG AGACGAAAAA GCGGCAGAAG 
GACCTGCCCA ACCTGCAGGC GGGCAAGAAA GACTCCAGCA AGAGATCAAA CAGCAGTGAC 
GAAAACACGG TGGTGAATCA CGCCTCTGGC GGCCTGCTGG CCTTCGCCCA GGTGGGCGGA 
AACGACCATG ACACCTGGCG ACGTCCGCGC TCCGAGAGTT CACCGGACGC GGAGAGTGTC 
TACATGACCA ACATGGTCAT CGACTCCGGC TACCACGGGA TGCACTCGCG CAAGTCAAGC 
ATCAAAAGCA CGAATACAAT CAAAAAATCG TACACCTGCT TCGGCAGCAT CAAGGAGTGG 
TGCATTGCGT GGTGGCACTC CGGTCGCGAG GACTCCGACG ACTTCGCCTA CGAGCAGGAG 
GAGCCTTCTG ATTTAGGGTA TGCAACACCA GTAACAATTG AAACTCCTTT ACAAAGCTCC 
GTCTCCAGAT GCACCTCGAT GAACGTGATG CGGGACAACT ACTCGATGGG CGGCAGCGTG 
AGCGGCGTGC GTCCGCCCAG CATTCTCCTG TCGGATGTCT CGCCCACGCC GCTGCCACGC 
CCACCGCTTG CCTCCATCTC GCAGCTGCAG GAAATGAGTG CGGTTACGGC GAGCACAACG 
GCGAATGTGA ATACCAGTGG CAATGGCAAT GGCGCCATCA ACAACAACAA CAACGCCAGC 
CACAACGGGA ACGGCGCAGT GAACGGGAAT GGGGCCGGAA ACGGAAGTGG AATCGGACTG 
GGTACAACCG GAAATGCGAC GCACCGCGAC TCGCGCACTC TGCCCGTTAT CAATCGCATT 
AACTCACGGT CGGTGAGCCA GGACTCCGTG TACACGATAC TCATCCGCCT GCCTTCCGAC 
GGCGCATCCA GCAACGCGGC CAACGGAGGA GGGGGTGGCC CTGGTGCCGG GGCAGCTGCC 
TCCGCGTCCT TGAGCATGCA AGGCGACTGT GCGCCCTCCA TCAAGATGAT ACACGAGGAC 
GGGCCCACGA CGACGGCAGC GGCAGCTCCT TTGGCAAGCG CCGCAGCCAC ACGACGACCT 
TTGCCCTCGC GAGACTCTGA GTTCAGCCTG CCACTCGGAC GGAGGATGTC CCATGCCCAG 
CACGATGCCA GGCTACTCAA CGCCAAGGTC ATACCCAAGC AGCTGGGCAA GGCTGGGGGC 
GGGGCAGCGG GCGGCGGAGT GGGCGGAGCC CACGCCCTCA TGAACGCCCG CAATGCTGCC 
AAGAAGAAAA AGAAATCGCA AGAGAAGCGG CAGGAGTCGA AGGCGGCCAA GACGCTGTCG 
GCTATCCTGC TGAGCTTCAT CATCACGTGG ACTCCGTACA ACATCCTGGT GCTGATCAAG 
CCGCTGACCA CCTGCTCCGA CTGCATACCC ACGGAGCTGT GGGACTTTTT CTACGCCCTG 
TGCTACATCA ACTCGACGAT CAACCCCATG TGCTACGCCT TGTGCAACGC CACCTTCCGG 
AGAACCTACG TCCGGATCCT CACCTGCAAG TGGCACACCC GGAACCGCGA GGGCATGGTA 
CGCGGCGTCT ACAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523844.2
CDS	871..3288
Translation	MEPVMSLALAAHGPPSILEPLFKTVTTSTTTTTTTTTSTTTTTASPAGYSPGYPGTTLLTALFENLTSTAASGLYDPYSGMYGNQTNGTIGFETKGPRYSLASMVVMGFVAAILSTVTVAGNVMVMISFKIDKQLQTISNYFLFSLAIADFAIGAISMPLFAVTTILGYWPLGPIVCDTWLALDYLASNASVLNLLIISFDRYFSVTRPLTYRAKRTTNRAAVMIGAAWGISLLLWPPWIYSWPYIEGKRTVPKDECYIQFIETNQYITFGTALAAFYFPVTIMCFLYWRIWRETKKRQKDLPNLQAGKKDSSKRSNSSDENTVVNHASGGLLAFAQVGGNDHDTWRRPRSESSPDAESVYMTNMVIDSGYHGMHSRKSSIKSTNTIKKSYTCFGSIKEWCIAWWHSGREDSDDFAYEQEEPSDLGYATPVTIETPLQSSVSRCTSMNVMRDNYSMGGSVSGVRPPSILLSDVSPTPLPRPPLASISQLQEMSAVTASTTANVNTSGNGNGAINNNNNASHNGNGAVNGNGAGNGSGIGLGTTGNATHRDSRTLPVINRINSRSVSQDSVYTILIRLPSDGASSNAANGGGGGPGAGAAASASLSMQGDCAPSIKMIHEDGPTTTAAAAPLASAAATRRPLPSRDSEFSLPLGRRMSHAQHDARLLNAKVIPKQLGKAGGGAAGGGVGGAHALMNARNAAKKKKKSQEKRQESKAAKTLSAILLSFIITWTPYNILVLIKPLTTCSDCIPTELWDFFYALCYINSTINPMCYALCNATFRRTYVRILTCKWHTRNREGMVRGVYN

Target ORF information:

RefSeq Version	NM_079120.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster muscarinic acetylcholine Receptor, A-type, transcript variant B (mAChR-A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079120.3

ORF Insert Sequence:

ATGGAGCCGG TCATGAGTCT GGCATTGGCC GCCCATGGAC CGCCTAGCAT ACTAGAGCCG 
CTGTTTAAGA CCGTAACCAC GAGCACAACG ACCACCACCA CCACGACGAC CAGCACGACG 
ACGACGACGG CGAGTCCTGC AGGGTATTCG CCAGGATACC CGGGCACCAC TCTTCTGACC 
GCCCTGTTCG AGAACCTGAC GTCCACGGCG GCGAGCGGGC TATACGATCC CTATTCGGGA 
ATGTACGGAA ACCAGACGAA CGGCACCATC GGCTTCGAGA CAAAGGGACC TCGGTACTCA 
CTGGCCTCCA TGGTGGTCAT GGGCTTCGTG GCGGCGATTT TGAGCACGGT GACGGTGGCG 
GGCAATGTCA TGGTGATGAT ATCCTTTAAG ATCGACAAGC AGCTGCAGAC GATCAGCAAC 
TACTTCTTGT TCTCGCTGGC CATCGCGGAC TTCGCCATTG GCGCTATATC GATGCCGCTT 
TTTGCGGTGA CCACAATCCT GGGCTACTGG CCCTTGGGTC CCATCGTCTG CGACACCTGG 
CTGGCCCTGG ACTACCTGGC ATCGAATGCT TCAGTGCTGA ACCTGCTGAT CATCAGTTTC 
GATCGGTACT TCAGTGTCAC TCGACCGCTG ACGTACCGCG CCAAAAGGAC CACCAACCGG 
GCGGCGGTGA TGATTGGCGC CGCCTGGGGC ATCAGCCTGC TCCTCTGGCC GCCCTGGATC 
TACAGCTGGC CCTACATCGA GGGCAAGCGG ACGGTGCCCA AGGACGAGTG CTACATCCAG 
TTCATCGAGA CCAACCAGTA CATCACCTTC GGCACGGCAC TGGCCGCCTT CTACTTCCCG 
GTCACCATCA TGTGCTTCCT CTACTGGCGC ATCTGGCGCG AGACGAAAAA GCGGCAGAAG 
GACCTGCCCA ACCTGCAGGC GGGCAAGAAA GACTCCAGCA AGAGATCAAA CAGCAGTGAC 
GAAAACACGG TGGTGAATCA CGCCTCTGGC GGCCTGCTGG CCTTCGCCCA GGTGGGCGGA 
AACGACCATG ACACCTGGCG ACGTCCGCGC TCCGAGAGTT CACCGGACGC GGAGAGTGTC 
TACATGACCA ACATGGTCAT CGACTCCGGC TACCACGGGA TGCACTCGCG CAAGTCAAGC 
ATCAAAAGCA CGAATACAAT CAAAAAATCG TACACCTGCT TCGGCAGCAT CAAGGAGTGG 
TGCATTGCGT GGTGGCACTC CGGTCGCGAG GACTCCGACG ACTTCGCCTA CGAGCAGGAG 
GAGCCTTCTG ATTTAGGGTA TGCAACACCA GTAACAATTG AAACTCCTTT ACAAAGCTCC 
GTCTCCAGAT GCACCTCGAT GAACGTGATG CGGGACAACT ACTCGATGGG CGGCAGCGTG 
AGCGGCGTGC GTCCGCCCAG CATTCTCCTG TCGGATGTCT CGCCCACGCC GCTGCCACGC 
CCACCGCTTG CCTCCATCTC GCAGCTGCAG GAAATGAGTG CGGTTACGGC GAGCACAACG 
GCGAATGTGA ATACCAGTGG CAATGGCAAT GGCGCCATCA ACAACAACAA CAACGCCAGC 
CACAACGGGA ACGGCGCAGT GAACGGGAAT GGGGCCGGAA ACGGAAGTGG AATCGGACTG 
GGTACAACCG GAAATGCGAC GCACCGCGAC TCGCGCACTC TGCCCGTTAT CAATCGCATT 
AACTCACGGT CGGTGAGCCA GGACTCCGTG TACACGATAC TCATCCGCCT GCCTTCCGAC 
GGCGCATCCA GCAACGCGGC CAACGGAGGA GGGGGTGGCC CTGGTGCCGG GGCAGCTGCC 
TCCGCGTCCT TGAGCATGCA AGGCGACTGT GCGCCCTCCA TCAAGATGAT ACACGAGGAC 
GGGCCCACGA CGACGGCAGC GGCAGCTCCT TTGGCAAGCG CCGCAGCCAC ACGACGACCT 
TTGCCCTCGC GAGACTCTGA GTTCAGCCTG CCACTCGGAC GGAGGATGTC CCATGCCCAG 
CACGATGCCA GGCTACTCAA CGCCAAGGTC ATACCCAAGC AGCTGGGCAA GGCTGGGGGC 
GGGGCAGCGG GCGGCGGAGT GGGCGGAGCC CACGCCCTCA TGAACGCCCG CAATGCTGCC 
AAGAAGAAAA AGAAATCGCA AGAGAAGCGG CAGGAGTCGA AGGCGGCCAA GACGCTGTCG 
GCTATCCTGC TGAGCTTCAT CATCACGTGG ACTCCGTACA ACATCCTGGT GCTGATCAAG 
CCGCTGACCA CCTGCTCCGA CTGCATACCC ACGGAGCTGT GGGACTTTTT CTACGCCCTG 
TGCTACATCA ACTCGACGAT CAACCCCATG TGCTACGCCT TGTGCAACGC CACCTTCCGG 
AGAACCTACG TCCGGATCCT CACCTGCAAG TGGCACACCC GGAACCGCGA GGGCATGGTA 
CGCGGCGTCT ACAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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mAChR-A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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