Nop60B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nop60B
Entrez Gene ID	37873
Full Name	Nucleolar protein at 60B
Synonyms	CG333,CG3333,Dmel\CG3333,FBgn0023184,MFL/Nop60B,Nop60,Nop60B/mfl,Nop60B/mnf,Nop60b,Uhg6,dUhg6,l(2)k05318,mfl,minifly,nop60B
General protein information	Preferred Names Nucleolar protein at 60B Names CG3333 gene product from transcript CG3333-RF CG3333-PA CG3333-PB CG3333-PC CG3333-PF CG3333-PG Nop60B-PA Nop60B-PB Nop60B-PC Nop60B-PF Nop60B-PG Uhg6 dUhg-like 6 minifly nucleolar protein at 60B
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 60C1-60C2

mRNA and Protein(s)

mRNA	Protein	Name
NM_080381.5	NP_525120.1	nucleolar protein at 60B, isoform A
NM_001014547.4	NP_001014547.1	nucleolar protein at 60B, isoform B
NM_001169818.3	NP_001163289.1	nucleolar protein at 60B, isoform F
NM_001259561.2	NP_001246490.1	nucleolar protein at 60B, isoform G
NM_001169815.3	NP_001163286.1	nucleolar protein at 60B, isoform C

Pathways from BioSystems

Homologies

	DKC1	XP_001090867.2
Mus musculus (house mouse)	Dkc1	NP_001025478.1
Gallus gallus (chicken)	DKC1	NP_001026286.1
Caenorhabditis elegans (roundworm)	K01G5.5	NP_499370.1
Magnaporthe oryzae (rice blast fungus)	MGG_07518	XP_003711432.1
Oryza sativa (rice)	Os07g0636000	NP_001060391.1
Rattus norvegicus (Norway rat)	Dkc1	NP_596910.1
Drosophila melanogaster (fruit fly)	Nop60B	NP_001163289.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004739	XP_318082.4
Kluyveromyces lactis	KLLA0D04796g	XP_453273.1
Eremothecium gossypii	AGOS_AAL007C	NP_982535.1
Pan troglodytes (chimpanzee)	DKC1	XP_521345.3
Bos taurus (cattle)	DKC1	NP_001098865.1
Saccharomyces cerevisiae (baker's yeast)	CBF5	NP_013276.1
Neurospora crassa	NCU01290	XP_960827.1
Arabidopsis thaliana (thale cress)	NAP57	NP_191274.1
Xenopus tropicalis (tropical clawed frog)	dkc1	XP_004916830.1
Canis lupus familiaris (dog)	DKC1	XP_549382.2
Danio rerio (zebrafish)	dkc1	NP_001028279.2
Schizosaccharomyces pombe (fission yeast)	cbf5	NP_594878.1
Oryza sativa (rice)	Os03g0370500	NP_001050199.1
Homo sapiens (human)	DKC1	NP_001354.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Pseudouridine synthase (diskerin,Mfl)depletion also causes disruption of adherens cell junctions and ectopic induction of JNK (c-Jun N-terminal kinase) and Mmp1 (matrix metalloproteinase-1) activity, leading to a significant epithelial reorganization
Title: Loss of Drosophila pseudouridine synthase triggers apoptosis-induced proliferation and promotes cell-nonautonomous EMT.

results expand repertoire of coding/non-coding transcripts derived from the gene encoding Drosophila pseudouridine synthase It exhibits complex interlaced organization.
Title: The coding/non-coding overlapping architecture of the gene encoding the Drosophila pseudouridine synthase.

Mutations in Nop60B, the Drosophila homolog of human dyskeratosis congenita 1, affect the maintenance of the germ-line stem cell lineage during spermatogenesis.
Title: Mutations in Nop60B, the Drosophila homolog of human dyskeratosis congenita 1, affect the maintenance of the germ-line stem cell lineage during spermatogenesis.

The following Nop60B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nop60B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13230	NM_001259561.2 Latest version!	Drosophila melanogaster nucleolar protein at 60B, transcript variant G (Nop60B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa11139	NM_080381.5 Latest version!	Drosophila melanogaster nucleolar protein at 60B, transcript variant A (Nop60B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa11139	NM_001169815.3 Latest version!	Drosophila melanogaster nucleolar protein at 60B, transcript variant C (Nop60B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa11139	NM_001169818.3 Latest version!	Drosophila melanogaster nucleolar protein at 60B, transcript variant F (Nop60B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa11139	NM_001014547.4 Latest version!	Drosophila melanogaster nucleolar protein at 60B, transcript variant B (Nop60B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nop60B ( NM_001259561.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246490.1 Drosophila melanogaster nucleolar protein at 60B, transcript variant G (Nop60B), mRNA.

Price & Availability↑

CloneID	OFa13230
Clone ID Related Accession (Same CDS sequence)	NM_001259561.2
Accession Version	NM_001259561.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nucleolar protein at 60B, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001259561.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGACG TGGAAGTACG CAAGGAGAAG AAAAAGAAGA AGATCAAGGA GGAGCCCTTG 
GACGGCGATG ACATTGGCAC GCTGCAGAAG CAGGGCAACT TCCAAATCAA GCCCTCCTCC 
AAGATCGCCG AGCTGGACAC CTCGCAATGG CCGCTGCTCC TGAAGAACTT CGACAAGTTG 
AACATCCGTT CCAACCACTA CACTCCGCTG GCGCACGGAT CGTCGCCCCT GAACCGCGAC 
ATCAAAGAGT ACATGAAGAC TGGCTTCATC AACCTGGACA AGCCGTCGAA CCCCAGCTCC 
CACGAGGTGG TGGCCTGGAT CAAGAAAATC CTCAAGGTGG AGAAGACAGG CCACTCCGGC 
ACGCTGGATC CCAAAGTCAC GGGTTGCCTG ATTGTTTGTA TTGACAGGGC CACCCGTCTG 
GTGAAGTCCC AGCAGAGCGC CGGCAAGGAG TACGTGGCCA TCTTCAAGCT GCACGGAGCC 
GTGGAGTCGG TGGCCAAGGT GCGTCAGGGC CTGGAGAAGC TGCGTGGTGC CCTCTTCCAG 
AGACCTCCAC TCATCTCCGC CGTAAAGCGT CAGTTGCGCG TTCGTACTGT CTACGACAGC 
AAGCTGTTGG ACTACGATGA AACCCGAAAT ATGGATTTGT TGATTAGGTT ATGCAATATA 
TGGACTATAA CTTTAATAAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246490.1
CDS	79..762
Translation	MADVEVRKEKKKKKIKEEPLDGDDIGTLQKQGNFQIKPSSKIAELDTSQWPLLLKNFDKLNIRSNHYTPLAHGSSPLNRDIKEYMKTGFINLDKPSNPSSHEVVAWIKKILKVEKTGHSGTLDPKVTGCLIVCIDRATRLVKSQQSAGKEYVAIFKLHGAVESVAKVRQGLEKLRGALFQRPPLISAVKRQLRVRTVYDSKLLDYDETRNMDLLIRLCNIWTITLIK

Target ORF information:

RefSeq Version	NM_001259561.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nucleolar protein at 60B, transcript variant G (Nop60B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259561.2

ORF Insert Sequence:

ATGGCCGACG TGGAAGTACG CAAGGAGAAG AAAAAGAAGA AGATCAAGGA GGAGCCCTTG 
GACGGCGATG ACATTGGCAC GCTGCAGAAG CAGGGCAACT TCCAAATCAA GCCCTCCTCC 
AAGATCGCCG AGCTGGACAC CTCGCAATGG CCGCTGCTCC TGAAGAACTT CGACAAGTTG 
AACATCCGTT CCAACCACTA CACTCCGCTG GCGCACGGAT CGTCGCCCCT GAACCGCGAC 
ATCAAAGAGT ACATGAAGAC TGGCTTCATC AACCTGGACA AGCCGTCGAA CCCCAGCTCC 
CACGAGGTGG TGGCCTGGAT CAAGAAAATC CTCAAGGTGG AGAAGACAGG CCACTCCGGC 
ACGCTGGATC CCAAAGTCAC GGGTTGCCTG ATTGTTTGTA TTGACAGGGC CACCCGTCTG 
GTGAAGTCCC AGCAGAGCGC CGGCAAGGAG TACGTGGCCA TCTTCAAGCT GCACGGAGCC 
GTGGAGTCGG TGGCCAAGGT GCGTCAGGGC CTGGAGAAGC TGCGTGGTGC CCTCTTCCAG 
AGACCTCCAC TCATCTCCGC CGTAAAGCGT CAGTTGCGCG TTCGTACTGT CTACGACAGC 
AAGCTGTTGG ACTACGATGA AACCCGAAAT ATGGATTTGT TGATTAGGTT ATGCAATATA 
TGGACTATAA CTTTAATAAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nop60B ( NM_080381.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_525120.1 Drosophila melanogaster nucleolar protein at 60B, transcript variant A (Nop60B), mRNA.

Price & Availability↑

CloneID	OFa11139
Clone ID Related Accession (Same CDS sequence)	NM_080381.5 , NM_001014547.4 , NM_001169818.3 , NM_001169815.3
Accession Version	NM_080381.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nucleolar protein at 60B, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_080381.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGACG TGGAAGTACG CAAGGAGAAG AAAAAGAAGA AGATCAAGGA GGAGCCCTTG 
GACGGCGATG ACATTGGCAC GCTGCAGAAG CAGGGCAACT TCCAAATCAA GCCCTCCTCC 
AAGATCGCCG AGCTGGACAC CTCGCAATGG CCGCTGCTCC TGAAGAACTT CGACAAGTTG 
AACATCCGTT CCAACCACTA CACTCCGCTG GCGCACGGAT CGTCGCCCCT GAACCGCGAC 
ATCAAAGAGT ACATGAAGAC TGGCTTCATC AACCTGGACA AGCCGTCGAA CCCCAGCTCC 
CACGAGGTGG TGGCCTGGAT CAAGAAAATC CTCAAGGTGG AGAAGACAGG CCACTCCGGC 
ACGCTGGATC CCAAAGTCAC GGGTTGCCTG ATTGTTTGTA TTGACAGGGC CACCCGTCTG 
GTGAAGTCCC AGCAGAGCGC CGGCAAGGAG TACGTGGCCA TCTTCAAGCT GCACGGAGCC 
GTGGAGTCGG TGGCCAAGGT GCGTCAGGGC CTGGAGAAGC TGCGTGGTGC CCTCTTCCAG 
AGACCTCCAC TCATCTCCGC CGTAAAGCGT CAGTTGCGCG TTCGTACTGT CTACGACAGC 
AAGCTGTTGG ACTACGATGA AACCCGAAAT ATGGGTGTTT TTTGGGTTAG TTGCGAGGCC 
GGCTCCTACA TCCGTACCAT GTGCGTCCAT CTTGGTCTCG TACTGGGTGT CGGTGGCCAG 
ATGCTGGAGC TCCGCCGTGT CCGCTCGGGC ATTCAGTCTG AGCGCGATGG TATGGTGACC 
ATGCACGATG TTTTGGACGC CATGTGGCTG TACGAAAACC ACAAGGACGA GTCTATGCTG 
CGACGTGTGA TCAAGCCGCT AGAAGGTCTG CTGGTCAACC ACAAGCGCAT TATTATGAAG 
GACAGTTCGG TCAACGCCGT TTGCTATGGT GCCAAGATTA CATTGCCCGG TGTCCTGCGC 
TACGAGGATG GCATTGAAAT CGATCAGGAG ATCGTCATTT GCACCACCAA GGGTGAGGCC 
ATTTGCCTGG CCATCGCCCT CATGACCACA GCCACTATGG CCTCTTGTGA CCATGGTGTG 
GTGGCCAAGA TCAAGCGAGT GATCATGGAG CGAGACACAT ACCCGCGCAA GTGGGGATTG 
GGACCAAAGG CGTCGGCCAA GAAGGCGCTC ATTGCCGCCG GCAAGCTAGA CAAGTTCGGA 
AGGCCCAATG AAAACACACC TAAGGAGTGG CTGACCGGTT ATGTCGACTA CAATGCCAAG 
AAGCCAGCAG CTCAAGAAGT CTCCCCCACA AATGGCTCCA GTGAACCCAG CAAACGCAAG 
TTAAGCACCT CCAGCGTTGA GGAAACTGCT GCGGCGGCGG TATCCGAGGA GACTCCTTCC 
AAGGACAAAA AGAAGAAGAA AAAGAAGCAC AAGGGCGACG AGGAAGCCCC AGAAGCCGCT 
GAGGAGGAGG CAGAGCCAGT AGAAAAGGAG AAGAAAAAGA AGAAGAAGAA GGACAAGGAT 
AGGGACAGAG ACGAAGCTCA GGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_525120.1
CDS	79..1605
Translation	MADVEVRKEKKKKKIKEEPLDGDDIGTLQKQGNFQIKPSSKIAELDTSQWPLLLKNFDKLNIRSNHYTPLAHGSSPLNRDIKEYMKTGFINLDKPSNPSSHEVVAWIKKILKVEKTGHSGTLDPKVTGCLIVCIDRATRLVKSQQSAGKEYVAIFKLHGAVESVAKVRQGLEKLRGALFQRPPLISAVKRQLRVRTVYDSKLLDYDETRNMGVFWVSCEAGSYIRTMCVHLGLVLGVGGQMLELRRVRSGIQSERDGMVTMHDVLDAMWLYENHKDESMLRRVIKPLEGLLVNHKRIIMKDSSVNAVCYGAKITLPGVLRYEDGIEIDQEIVICTTKGEAICLAIALMTTATMASCDHGVVAKIKRVIMERDTYPRKWGLGPKASAKKALIAAGKLDKFGRPNENTPKEWLTGYVDYNAKKPAAQEVSPTNGSSEPSKRKLSTSSVEETAAAAVSEETPSKDKKKKKKKHKGDEEAPEAAEEEAEPVEKEKKKKKKKDKDRDRDEAQE

Target ORF information:

RefSeq Version	NM_080381.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nucleolar protein at 60B, transcript variant A (Nop60B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080381.5

ORF Insert Sequence:

ATGGCCGACG TGGAAGTACG CAAGGAGAAG AAAAAGAAGA AGATCAAGGA GGAGCCCTTG 
GACGGCGATG ACATTGGCAC GCTGCAGAAG CAGGGCAACT TCCAAATCAA GCCCTCCTCC 
AAGATCGCCG AGCTGGACAC CTCGCAATGG CCGCTGCTCC TGAAGAACTT CGACAAGTTG 
AACATCCGTT CCAACCACTA CACTCCGCTG GCGCACGGAT CGTCGCCCCT GAACCGCGAC 
ATCAAAGAGT ACATGAAGAC TGGCTTCATC AACCTGGACA AGCCGTCGAA CCCCAGCTCC 
CACGAGGTGG TGGCCTGGAT CAAGAAAATC CTCAAGGTGG AGAAGACAGG CCACTCCGGC 
ACGCTGGATC CCAAAGTCAC GGGTTGCCTG ATTGTTTGTA TTGACAGGGC CACCCGTCTG 
GTGAAGTCCC AGCAGAGCGC CGGCAAGGAG TACGTGGCCA TCTTCAAGCT GCACGGAGCC 
GTGGAGTCGG TGGCCAAGGT GCGTCAGGGC CTGGAGAAGC TGCGTGGTGC CCTCTTCCAG 
AGACCTCCAC TCATCTCCGC CGTAAAGCGT CAGTTGCGCG TTCGTACTGT CTACGACAGC 
AAGCTGTTGG ACTACGATGA AACCCGAAAT ATGGGTGTTT TTTGGGTTAG TTGCGAGGCC 
GGCTCCTACA TCCGTACCAT GTGCGTCCAT CTTGGTCTCG TACTGGGTGT CGGTGGCCAG 
ATGCTGGAGC TCCGCCGTGT CCGCTCGGGC ATTCAGTCTG AGCGCGATGG TATGGTGACC 
ATGCACGATG TTTTGGACGC CATGTGGCTG TACGAAAACC ACAAGGACGA GTCTATGCTG 
CGACGTGTGA TCAAGCCGCT AGAAGGTCTG CTGGTCAACC ACAAGCGCAT TATTATGAAG 
GACAGTTCGG TCAACGCCGT TTGCTATGGT GCCAAGATTA CATTGCCCGG TGTCCTGCGC 
TACGAGGATG GCATTGAAAT CGATCAGGAG ATCGTCATTT GCACCACCAA GGGTGAGGCC 
ATTTGCCTGG CCATCGCCCT CATGACCACA GCCACTATGG CCTCTTGTGA CCATGGTGTG 
GTGGCCAAGA TCAAGCGAGT GATCATGGAG CGAGACACAT ACCCGCGCAA GTGGGGATTG 
GGACCAAAGG CGTCGGCCAA GAAGGCGCTC ATTGCCGCCG GCAAGCTAGA CAAGTTCGGA 
AGGCCCAATG AAAACACACC TAAGGAGTGG CTGACCGGTT ATGTCGACTA CAATGCCAAG 
AAGCCAGCAG CTCAAGAAGT CTCCCCCACA AATGGCTCCA GTGAACCCAG CAAACGCAAG 
TTAAGCACCT CCAGCGTTGA GGAAACTGCT GCGGCGGCGG TATCCGAGGA GACTCCTTCC 
AAGGACAAAA AGAAGAAGAA AAAGAAGCAC AAGGGCGACG AGGAAGCCCC AGAAGCCGCT 
GAGGAGGAGG CAGAGCCAGT AGAAAAGGAG AAGAAAAAGA AGAAGAAGAA GGACAAGGAT 
AGGGACAGAG ACGAAGCTCA GGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nop60B ( NM_001014547.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014547.1 Drosophila melanogaster nucleolar protein at 60B, transcript variant B (Nop60B), mRNA.

Price & Availability↑

CloneID	OFa11139
Clone ID Related Accession (Same CDS sequence)	NM_080381.5 , NM_001014547.4 , NM_001169818.3 , NM_001169815.3
Accession Version	NM_001014547.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nucleolar protein at 60B, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001014547.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGACG TGGAAGTACG CAAGGAGAAG AAAAAGAAGA AGATCAAGGA GGAGCCCTTG 
GACGGCGATG ACATTGGCAC GCTGCAGAAG CAGGGCAACT TCCAAATCAA GCCCTCCTCC 
AAGATCGCCG AGCTGGACAC CTCGCAATGG CCGCTGCTCC TGAAGAACTT CGACAAGTTG 
AACATCCGTT CCAACCACTA CACTCCGCTG GCGCACGGAT CGTCGCCCCT GAACCGCGAC 
ATCAAAGAGT ACATGAAGAC TGGCTTCATC AACCTGGACA AGCCGTCGAA CCCCAGCTCC 
CACGAGGTGG TGGCCTGGAT CAAGAAAATC CTCAAGGTGG AGAAGACAGG CCACTCCGGC 
ACGCTGGATC CCAAAGTCAC GGGTTGCCTG ATTGTTTGTA TTGACAGGGC CACCCGTCTG 
GTGAAGTCCC AGCAGAGCGC CGGCAAGGAG TACGTGGCCA TCTTCAAGCT GCACGGAGCC 
GTGGAGTCGG TGGCCAAGGT GCGTCAGGGC CTGGAGAAGC TGCGTGGTGC CCTCTTCCAG 
AGACCTCCAC TCATCTCCGC CGTAAAGCGT CAGTTGCGCG TTCGTACTGT CTACGACAGC 
AAGCTGTTGG ACTACGATGA AACCCGAAAT ATGGGTGTTT TTTGGGTTAG TTGCGAGGCC 
GGCTCCTACA TCCGTACCAT GTGCGTCCAT CTTGGTCTCG TACTGGGTGT CGGTGGCCAG 
ATGCTGGAGC TCCGCCGTGT CCGCTCGGGC ATTCAGTCTG AGCGCGATGG TATGGTGACC 
ATGCACGATG TTTTGGACGC CATGTGGCTG TACGAAAACC ACAAGGACGA GTCTATGCTG 
CGACGTGTGA TCAAGCCGCT AGAAGGTCTG CTGGTCAACC ACAAGCGCAT TATTATGAAG 
GACAGTTCGG TCAACGCCGT TTGCTATGGT GCCAAGATTA CATTGCCCGG TGTCCTGCGC 
TACGAGGATG GCATTGAAAT CGATCAGGAG ATCGTCATTT GCACCACCAA GGGTGAGGCC 
ATTTGCCTGG CCATCGCCCT CATGACCACA GCCACTATGG CCTCTTGTGA CCATGGTGTG 
GTGGCCAAGA TCAAGCGAGT GATCATGGAG CGAGACACAT ACCCGCGCAA GTGGGGATTG 
GGACCAAAGG CGTCGGCCAA GAAGGCGCTC ATTGCCGCCG GCAAGCTAGA CAAGTTCGGA 
AGGCCCAATG AAAACACACC TAAGGAGTGG CTGACCGGTT ATGTCGACTA CAATGCCAAG 
AAGCCAGCAG CTCAAGAAGT CTCCCCCACA AATGGCTCCA GTGAACCCAG CAAACGCAAG 
TTAAGCACCT CCAGCGTTGA GGAAACTGCT GCGGCGGCGG TATCCGAGGA GACTCCTTCC 
AAGGACAAAA AGAAGAAGAA AAAGAAGCAC AAGGGCGACG AGGAAGCCCC AGAAGCCGCT 
GAGGAGGAGG CAGAGCCAGT AGAAAAGGAG AAGAAAAAGA AGAAGAAGAA GGACAAGGAT 
AGGGACAGAG ACGAAGCTCA GGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014547.1
CDS	79..1605
Translation	MADVEVRKEKKKKKIKEEPLDGDDIGTLQKQGNFQIKPSSKIAELDTSQWPLLLKNFDKLNIRSNHYTPLAHGSSPLNRDIKEYMKTGFINLDKPSNPSSHEVVAWIKKILKVEKTGHSGTLDPKVTGCLIVCIDRATRLVKSQQSAGKEYVAIFKLHGAVESVAKVRQGLEKLRGALFQRPPLISAVKRQLRVRTVYDSKLLDYDETRNMGVFWVSCEAGSYIRTMCVHLGLVLGVGGQMLELRRVRSGIQSERDGMVTMHDVLDAMWLYENHKDESMLRRVIKPLEGLLVNHKRIIMKDSSVNAVCYGAKITLPGVLRYEDGIEIDQEIVICTTKGEAICLAIALMTTATMASCDHGVVAKIKRVIMERDTYPRKWGLGPKASAKKALIAAGKLDKFGRPNENTPKEWLTGYVDYNAKKPAAQEVSPTNGSSEPSKRKLSTSSVEETAAAAVSEETPSKDKKKKKKKHKGDEEAPEAAEEEAEPVEKEKKKKKKKDKDRDRDEAQE

Target ORF information:

RefSeq Version	NM_001014547.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nucleolar protein at 60B, transcript variant B (Nop60B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014547.4

ORF Insert Sequence:

ATGGCCGACG TGGAAGTACG CAAGGAGAAG AAAAAGAAGA AGATCAAGGA GGAGCCCTTG 
GACGGCGATG ACATTGGCAC GCTGCAGAAG CAGGGCAACT TCCAAATCAA GCCCTCCTCC 
AAGATCGCCG AGCTGGACAC CTCGCAATGG CCGCTGCTCC TGAAGAACTT CGACAAGTTG 
AACATCCGTT CCAACCACTA CACTCCGCTG GCGCACGGAT CGTCGCCCCT GAACCGCGAC 
ATCAAAGAGT ACATGAAGAC TGGCTTCATC AACCTGGACA AGCCGTCGAA CCCCAGCTCC 
CACGAGGTGG TGGCCTGGAT CAAGAAAATC CTCAAGGTGG AGAAGACAGG CCACTCCGGC 
ACGCTGGATC CCAAAGTCAC GGGTTGCCTG ATTGTTTGTA TTGACAGGGC CACCCGTCTG 
GTGAAGTCCC AGCAGAGCGC CGGCAAGGAG TACGTGGCCA TCTTCAAGCT GCACGGAGCC 
GTGGAGTCGG TGGCCAAGGT GCGTCAGGGC CTGGAGAAGC TGCGTGGTGC CCTCTTCCAG 
AGACCTCCAC TCATCTCCGC CGTAAAGCGT CAGTTGCGCG TTCGTACTGT CTACGACAGC 
AAGCTGTTGG ACTACGATGA AACCCGAAAT ATGGGTGTTT TTTGGGTTAG TTGCGAGGCC 
GGCTCCTACA TCCGTACCAT GTGCGTCCAT CTTGGTCTCG TACTGGGTGT CGGTGGCCAG 
ATGCTGGAGC TCCGCCGTGT CCGCTCGGGC ATTCAGTCTG AGCGCGATGG TATGGTGACC 
ATGCACGATG TTTTGGACGC CATGTGGCTG TACGAAAACC ACAAGGACGA GTCTATGCTG 
CGACGTGTGA TCAAGCCGCT AGAAGGTCTG CTGGTCAACC ACAAGCGCAT TATTATGAAG 
GACAGTTCGG TCAACGCCGT TTGCTATGGT GCCAAGATTA CATTGCCCGG TGTCCTGCGC 
TACGAGGATG GCATTGAAAT CGATCAGGAG ATCGTCATTT GCACCACCAA GGGTGAGGCC 
ATTTGCCTGG CCATCGCCCT CATGACCACA GCCACTATGG CCTCTTGTGA CCATGGTGTG 
GTGGCCAAGA TCAAGCGAGT GATCATGGAG CGAGACACAT ACCCGCGCAA GTGGGGATTG 
GGACCAAAGG CGTCGGCCAA GAAGGCGCTC ATTGCCGCCG GCAAGCTAGA CAAGTTCGGA 
AGGCCCAATG AAAACACACC TAAGGAGTGG CTGACCGGTT ATGTCGACTA CAATGCCAAG 
AAGCCAGCAG CTCAAGAAGT CTCCCCCACA AATGGCTCCA GTGAACCCAG CAAACGCAAG 
TTAAGCACCT CCAGCGTTGA GGAAACTGCT GCGGCGGCGG TATCCGAGGA GACTCCTTCC 
AAGGACAAAA AGAAGAAGAA AAAGAAGCAC AAGGGCGACG AGGAAGCCCC AGAAGCCGCT 
GAGGAGGAGG CAGAGCCAGT AGAAAAGGAG AAGAAAAAGA AGAAGAAGAA GGACAAGGAT 
AGGGACAGAG ACGAAGCTCA GGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nop60B ( NM_001169818.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163289.1 Drosophila melanogaster nucleolar protein at 60B, transcript variant F (Nop60B), mRNA.

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CloneID	OFa11139
Clone ID Related Accession (Same CDS sequence)	NM_080381.5 , NM_001014547.4 , NM_001169818.3 , NM_001169815.3
Accession Version	NM_001169818.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nucleolar protein at 60B, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001169818.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGACG TGGAAGTACG CAAGGAGAAG AAAAAGAAGA AGATCAAGGA GGAGCCCTTG 
GACGGCGATG ACATTGGCAC GCTGCAGAAG CAGGGCAACT TCCAAATCAA GCCCTCCTCC 
AAGATCGCCG AGCTGGACAC CTCGCAATGG CCGCTGCTCC TGAAGAACTT CGACAAGTTG 
AACATCCGTT CCAACCACTA CACTCCGCTG GCGCACGGAT CGTCGCCCCT GAACCGCGAC 
ATCAAAGAGT ACATGAAGAC TGGCTTCATC AACCTGGACA AGCCGTCGAA CCCCAGCTCC 
CACGAGGTGG TGGCCTGGAT CAAGAAAATC CTCAAGGTGG AGAAGACAGG CCACTCCGGC 
ACGCTGGATC CCAAAGTCAC GGGTTGCCTG ATTGTTTGTA TTGACAGGGC CACCCGTCTG 
GTGAAGTCCC AGCAGAGCGC CGGCAAGGAG TACGTGGCCA TCTTCAAGCT GCACGGAGCC 
GTGGAGTCGG TGGCCAAGGT GCGTCAGGGC CTGGAGAAGC TGCGTGGTGC CCTCTTCCAG 
AGACCTCCAC TCATCTCCGC CGTAAAGCGT CAGTTGCGCG TTCGTACTGT CTACGACAGC 
AAGCTGTTGG ACTACGATGA AACCCGAAAT ATGGGTGTTT TTTGGGTTAG TTGCGAGGCC 
GGCTCCTACA TCCGTACCAT GTGCGTCCAT CTTGGTCTCG TACTGGGTGT CGGTGGCCAG 
ATGCTGGAGC TCCGCCGTGT CCGCTCGGGC ATTCAGTCTG AGCGCGATGG TATGGTGACC 
ATGCACGATG TTTTGGACGC CATGTGGCTG TACGAAAACC ACAAGGACGA GTCTATGCTG 
CGACGTGTGA TCAAGCCGCT AGAAGGTCTG CTGGTCAACC ACAAGCGCAT TATTATGAAG 
GACAGTTCGG TCAACGCCGT TTGCTATGGT GCCAAGATTA CATTGCCCGG TGTCCTGCGC 
TACGAGGATG GCATTGAAAT CGATCAGGAG ATCGTCATTT GCACCACCAA GGGTGAGGCC 
ATTTGCCTGG CCATCGCCCT CATGACCACA GCCACTATGG CCTCTTGTGA CCATGGTGTG 
GTGGCCAAGA TCAAGCGAGT GATCATGGAG CGAGACACAT ACCCGCGCAA GTGGGGATTG 
GGACCAAAGG CGTCGGCCAA GAAGGCGCTC ATTGCCGCCG GCAAGCTAGA CAAGTTCGGA 
AGGCCCAATG AAAACACACC TAAGGAGTGG CTGACCGGTT ATGTCGACTA CAATGCCAAG 
AAGCCAGCAG CTCAAGAAGT CTCCCCCACA AATGGCTCCA GTGAACCCAG CAAACGCAAG 
TTAAGCACCT CCAGCGTTGA GGAAACTGCT GCGGCGGCGG TATCCGAGGA GACTCCTTCC 
AAGGACAAAA AGAAGAAGAA AAAGAAGCAC AAGGGCGACG AGGAAGCCCC AGAAGCCGCT 
GAGGAGGAGG CAGAGCCAGT AGAAAAGGAG AAGAAAAAGA AGAAGAAGAA GGACAAGGAT 
AGGGACAGAG ACGAAGCTCA GGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163289.1
CDS	79..1605
Translation	MADVEVRKEKKKKKIKEEPLDGDDIGTLQKQGNFQIKPSSKIAELDTSQWPLLLKNFDKLNIRSNHYTPLAHGSSPLNRDIKEYMKTGFINLDKPSNPSSHEVVAWIKKILKVEKTGHSGTLDPKVTGCLIVCIDRATRLVKSQQSAGKEYVAIFKLHGAVESVAKVRQGLEKLRGALFQRPPLISAVKRQLRVRTVYDSKLLDYDETRNMGVFWVSCEAGSYIRTMCVHLGLVLGVGGQMLELRRVRSGIQSERDGMVTMHDVLDAMWLYENHKDESMLRRVIKPLEGLLVNHKRIIMKDSSVNAVCYGAKITLPGVLRYEDGIEIDQEIVICTTKGEAICLAIALMTTATMASCDHGVVAKIKRVIMERDTYPRKWGLGPKASAKKALIAAGKLDKFGRPNENTPKEWLTGYVDYNAKKPAAQEVSPTNGSSEPSKRKLSTSSVEETAAAAVSEETPSKDKKKKKKKHKGDEEAPEAAEEEAEPVEKEKKKKKKKDKDRDRDEAQE

Target ORF information:

RefSeq Version	NM_001169818.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nucleolar protein at 60B, transcript variant F (Nop60B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169818.3

ORF Insert Sequence:

ATGGCCGACG TGGAAGTACG CAAGGAGAAG AAAAAGAAGA AGATCAAGGA GGAGCCCTTG 
GACGGCGATG ACATTGGCAC GCTGCAGAAG CAGGGCAACT TCCAAATCAA GCCCTCCTCC 
AAGATCGCCG AGCTGGACAC CTCGCAATGG CCGCTGCTCC TGAAGAACTT CGACAAGTTG 
AACATCCGTT CCAACCACTA CACTCCGCTG GCGCACGGAT CGTCGCCCCT GAACCGCGAC 
ATCAAAGAGT ACATGAAGAC TGGCTTCATC AACCTGGACA AGCCGTCGAA CCCCAGCTCC 
CACGAGGTGG TGGCCTGGAT CAAGAAAATC CTCAAGGTGG AGAAGACAGG CCACTCCGGC 
ACGCTGGATC CCAAAGTCAC GGGTTGCCTG ATTGTTTGTA TTGACAGGGC CACCCGTCTG 
GTGAAGTCCC AGCAGAGCGC CGGCAAGGAG TACGTGGCCA TCTTCAAGCT GCACGGAGCC 
GTGGAGTCGG TGGCCAAGGT GCGTCAGGGC CTGGAGAAGC TGCGTGGTGC CCTCTTCCAG 
AGACCTCCAC TCATCTCCGC CGTAAAGCGT CAGTTGCGCG TTCGTACTGT CTACGACAGC 
AAGCTGTTGG ACTACGATGA AACCCGAAAT ATGGGTGTTT TTTGGGTTAG TTGCGAGGCC 
GGCTCCTACA TCCGTACCAT GTGCGTCCAT CTTGGTCTCG TACTGGGTGT CGGTGGCCAG 
ATGCTGGAGC TCCGCCGTGT CCGCTCGGGC ATTCAGTCTG AGCGCGATGG TATGGTGACC 
ATGCACGATG TTTTGGACGC CATGTGGCTG TACGAAAACC ACAAGGACGA GTCTATGCTG 
CGACGTGTGA TCAAGCCGCT AGAAGGTCTG CTGGTCAACC ACAAGCGCAT TATTATGAAG 
GACAGTTCGG TCAACGCCGT TTGCTATGGT GCCAAGATTA CATTGCCCGG TGTCCTGCGC 
TACGAGGATG GCATTGAAAT CGATCAGGAG ATCGTCATTT GCACCACCAA GGGTGAGGCC 
ATTTGCCTGG CCATCGCCCT CATGACCACA GCCACTATGG CCTCTTGTGA CCATGGTGTG 
GTGGCCAAGA TCAAGCGAGT GATCATGGAG CGAGACACAT ACCCGCGCAA GTGGGGATTG 
GGACCAAAGG CGTCGGCCAA GAAGGCGCTC ATTGCCGCCG GCAAGCTAGA CAAGTTCGGA 
AGGCCCAATG AAAACACACC TAAGGAGTGG CTGACCGGTT ATGTCGACTA CAATGCCAAG 
AAGCCAGCAG CTCAAGAAGT CTCCCCCACA AATGGCTCCA GTGAACCCAG CAAACGCAAG 
TTAAGCACCT CCAGCGTTGA GGAAACTGCT GCGGCGGCGG TATCCGAGGA GACTCCTTCC 
AAGGACAAAA AGAAGAAGAA AAAGAAGCAC AAGGGCGACG AGGAAGCCCC AGAAGCCGCT 
GAGGAGGAGG CAGAGCCAGT AGAAAAGGAG AAGAAAAAGA AGAAGAAGAA GGACAAGGAT 
AGGGACAGAG ACGAAGCTCA GGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nop60B ( NM_001169815.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163286.1 Drosophila melanogaster nucleolar protein at 60B, transcript variant C (Nop60B), mRNA.

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CloneID	OFa11139
Clone ID Related Accession (Same CDS sequence)	NM_080381.5 , NM_001014547.4 , NM_001169818.3 , NM_001169815.3
Accession Version	NM_001169815.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nucleolar protein at 60B, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001169815.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGACG TGGAAGTACG CAAGGAGAAG AAAAAGAAGA AGATCAAGGA GGAGCCCTTG 
GACGGCGATG ACATTGGCAC GCTGCAGAAG CAGGGCAACT TCCAAATCAA GCCCTCCTCC 
AAGATCGCCG AGCTGGACAC CTCGCAATGG CCGCTGCTCC TGAAGAACTT CGACAAGTTG 
AACATCCGTT CCAACCACTA CACTCCGCTG GCGCACGGAT CGTCGCCCCT GAACCGCGAC 
ATCAAAGAGT ACATGAAGAC TGGCTTCATC AACCTGGACA AGCCGTCGAA CCCCAGCTCC 
CACGAGGTGG TGGCCTGGAT CAAGAAAATC CTCAAGGTGG AGAAGACAGG CCACTCCGGC 
ACGCTGGATC CCAAAGTCAC GGGTTGCCTG ATTGTTTGTA TTGACAGGGC CACCCGTCTG 
GTGAAGTCCC AGCAGAGCGC CGGCAAGGAG TACGTGGCCA TCTTCAAGCT GCACGGAGCC 
GTGGAGTCGG TGGCCAAGGT GCGTCAGGGC CTGGAGAAGC TGCGTGGTGC CCTCTTCCAG 
AGACCTCCAC TCATCTCCGC CGTAAAGCGT CAGTTGCGCG TTCGTACTGT CTACGACAGC 
AAGCTGTTGG ACTACGATGA AACCCGAAAT ATGGGTGTTT TTTGGGTTAG TTGCGAGGCC 
GGCTCCTACA TCCGTACCAT GTGCGTCCAT CTTGGTCTCG TACTGGGTGT CGGTGGCCAG 
ATGCTGGAGC TCCGCCGTGT CCGCTCGGGC ATTCAGTCTG AGCGCGATGG TATGGTGACC 
ATGCACGATG TTTTGGACGC CATGTGGCTG TACGAAAACC ACAAGGACGA GTCTATGCTG 
CGACGTGTGA TCAAGCCGCT AGAAGGTCTG CTGGTCAACC ACAAGCGCAT TATTATGAAG 
GACAGTTCGG TCAACGCCGT TTGCTATGGT GCCAAGATTA CATTGCCCGG TGTCCTGCGC 
TACGAGGATG GCATTGAAAT CGATCAGGAG ATCGTCATTT GCACCACCAA GGGTGAGGCC 
ATTTGCCTGG CCATCGCCCT CATGACCACA GCCACTATGG CCTCTTGTGA CCATGGTGTG 
GTGGCCAAGA TCAAGCGAGT GATCATGGAG CGAGACACAT ACCCGCGCAA GTGGGGATTG 
GGACCAAAGG CGTCGGCCAA GAAGGCGCTC ATTGCCGCCG GCAAGCTAGA CAAGTTCGGA 
AGGCCCAATG AAAACACACC TAAGGAGTGG CTGACCGGTT ATGTCGACTA CAATGCCAAG 
AAGCCAGCAG CTCAAGAAGT CTCCCCCACA AATGGCTCCA GTGAACCCAG CAAACGCAAG 
TTAAGCACCT CCAGCGTTGA GGAAACTGCT GCGGCGGCGG TATCCGAGGA GACTCCTTCC 
AAGGACAAAA AGAAGAAGAA AAAGAAGCAC AAGGGCGACG AGGAAGCCCC AGAAGCCGCT 
GAGGAGGAGG CAGAGCCAGT AGAAAAGGAG AAGAAAAAGA AGAAGAAGAA GGACAAGGAT 
AGGGACAGAG ACGAAGCTCA GGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163286.1
CDS	79..1605
Translation	MADVEVRKEKKKKKIKEEPLDGDDIGTLQKQGNFQIKPSSKIAELDTSQWPLLLKNFDKLNIRSNHYTPLAHGSSPLNRDIKEYMKTGFINLDKPSNPSSHEVVAWIKKILKVEKTGHSGTLDPKVTGCLIVCIDRATRLVKSQQSAGKEYVAIFKLHGAVESVAKVRQGLEKLRGALFQRPPLISAVKRQLRVRTVYDSKLLDYDETRNMGVFWVSCEAGSYIRTMCVHLGLVLGVGGQMLELRRVRSGIQSERDGMVTMHDVLDAMWLYENHKDESMLRRVIKPLEGLLVNHKRIIMKDSSVNAVCYGAKITLPGVLRYEDGIEIDQEIVICTTKGEAICLAIALMTTATMASCDHGVVAKIKRVIMERDTYPRKWGLGPKASAKKALIAAGKLDKFGRPNENTPKEWLTGYVDYNAKKPAAQEVSPTNGSSEPSKRKLSTSSVEETAAAAVSEETPSKDKKKKKKKHKGDEEAPEAAEEEAEPVEKEKKKKKKKDKDRDRDEAQE

Target ORF information:

RefSeq Version	NM_001169815.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nucleolar protein at 60B, transcript variant C (Nop60B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169815.3

ORF Insert Sequence:

ATGGCCGACG TGGAAGTACG CAAGGAGAAG AAAAAGAAGA AGATCAAGGA GGAGCCCTTG 
GACGGCGATG ACATTGGCAC GCTGCAGAAG CAGGGCAACT TCCAAATCAA GCCCTCCTCC 
AAGATCGCCG AGCTGGACAC CTCGCAATGG CCGCTGCTCC TGAAGAACTT CGACAAGTTG 
AACATCCGTT CCAACCACTA CACTCCGCTG GCGCACGGAT CGTCGCCCCT GAACCGCGAC 
ATCAAAGAGT ACATGAAGAC TGGCTTCATC AACCTGGACA AGCCGTCGAA CCCCAGCTCC 
CACGAGGTGG TGGCCTGGAT CAAGAAAATC CTCAAGGTGG AGAAGACAGG CCACTCCGGC 
ACGCTGGATC CCAAAGTCAC GGGTTGCCTG ATTGTTTGTA TTGACAGGGC CACCCGTCTG 
GTGAAGTCCC AGCAGAGCGC CGGCAAGGAG TACGTGGCCA TCTTCAAGCT GCACGGAGCC 
GTGGAGTCGG TGGCCAAGGT GCGTCAGGGC CTGGAGAAGC TGCGTGGTGC CCTCTTCCAG 
AGACCTCCAC TCATCTCCGC CGTAAAGCGT CAGTTGCGCG TTCGTACTGT CTACGACAGC 
AAGCTGTTGG ACTACGATGA AACCCGAAAT ATGGGTGTTT TTTGGGTTAG TTGCGAGGCC 
GGCTCCTACA TCCGTACCAT GTGCGTCCAT CTTGGTCTCG TACTGGGTGT CGGTGGCCAG 
ATGCTGGAGC TCCGCCGTGT CCGCTCGGGC ATTCAGTCTG AGCGCGATGG TATGGTGACC 
ATGCACGATG TTTTGGACGC CATGTGGCTG TACGAAAACC ACAAGGACGA GTCTATGCTG 
CGACGTGTGA TCAAGCCGCT AGAAGGTCTG CTGGTCAACC ACAAGCGCAT TATTATGAAG 
GACAGTTCGG TCAACGCCGT TTGCTATGGT GCCAAGATTA CATTGCCCGG TGTCCTGCGC 
TACGAGGATG GCATTGAAAT CGATCAGGAG ATCGTCATTT GCACCACCAA GGGTGAGGCC 
ATTTGCCTGG CCATCGCCCT CATGACCACA GCCACTATGG CCTCTTGTGA CCATGGTGTG 
GTGGCCAAGA TCAAGCGAGT GATCATGGAG CGAGACACAT ACCCGCGCAA GTGGGGATTG 
GGACCAAAGG CGTCGGCCAA GAAGGCGCTC ATTGCCGCCG GCAAGCTAGA CAAGTTCGGA 
AGGCCCAATG AAAACACACC TAAGGAGTGG CTGACCGGTT ATGTCGACTA CAATGCCAAG 
AAGCCAGCAG CTCAAGAAGT CTCCCCCACA AATGGCTCCA GTGAACCCAG CAAACGCAAG 
TTAAGCACCT CCAGCGTTGA GGAAACTGCT GCGGCGGCGG TATCCGAGGA GACTCCTTCC 
AAGGACAAAA AGAAGAAGAA AAAGAAGCAC AAGGGCGACG AGGAAGCCCC AGAAGCCGCT 
GAGGAGGAGG CAGAGCCAGT AGAAAAGGAG AAGAAAAAGA AGAAGAAGAA GGACAAGGAT 
AGGGACAGAG ACGAAGCTCA GGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nop60B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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