Naa35 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Naa35
Entrez Gene ID	37866
Full Name	CG4065 gene product from transcript CG4065-RB
Synonyms	Dmel\CG4065,anon-EST:Nelson1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 60B12-60B12

mRNA and Protein(s)

mRNA	Protein	Name
NM_138047.3	NP_611891.1	N(alpha)-acetyltransferase 35, isoform A
NM_001014546.2	NP_001014546.1	N(alpha)-acetyltransferase 35, isoform B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NAA35	XP_533507.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005863	XP_315888.4
Xenopus tropicalis (tropical clawed frog)	naa35	NP_001186425.1
Bos taurus (cattle)	NAA35	NP_001180040.1
Danio rerio (zebrafish)	naa35	NP_955844.1
Mus musculus (house mouse)	Naa35	NP_084429.2
Arabidopsis thaliana (thale cress)	MAK10	NP_001118295.1
Pan troglodytes (chimpanzee)	NAA35	XP_528341.2
Gallus gallus (chicken)	NAA35	NP_001026623.1
Homo sapiens (human)	NAA35	NP_078911.3
	NAA35	XP_001107941.1
Rattus norvegicus (Norway rat)	Naa35	NP_579858.1
Drosophila melanogaster (fruit fly)	CG4065	NP_611891.1
Caenorhabditis elegans (roundworm)	T23B12.4	NP_505179.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Naa35 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Naa35 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11132	NM_001014546.2 Latest version!	Drosophila melanogaster N(alpha)-acetyltransferase 35 (Naa35), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa11133	NM_138047.3 Latest version!	Drosophila melanogaster N(alpha)-acetyltransferase 35 (Naa35), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Naa35 ( NM_001014546.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014546.1 Drosophila melanogaster N(alpha)-acetyltransferase 35 (Naa35), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa11132
Clone ID Related Accession (Same CDS sequence)	NM_001014546.2
Accession Version	NM_001014546.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	N(alpha)-acetyltransferase 35, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001014546.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTATCCCT CGACCTTGCC GGCGGAGGCA GATCCGAGCA CCATGAACGG AAGCGTTGCA 
GAGCCTCCGG ACTTCCAGGC TGCCACGGCG GCCGCGGCGG CAGCTGCCCG GGCCAGTAGC 
TTGGAACAGG ACGAGTGGAG CGTGGGCGAG TGCGGATTTC TGGATCCGGA TGTGCAGCGC 
ACCATGCGGA GCGGCAGTGC TGCGGAGGAC ATCACCCAGT ACCCGATGCA CGGCTGGGTG 
GACGTAACCA AGGAGTTCCA CGATGCCTGC GCGGAGCTGC AGCCCGGGGA ACTGGCCCAG 
GATATGCTGT TCGGTCTTTT TGAGGCCATG TCAGCCATCG AGATCATGGA CCCCAAGATG 
GACGTGGGCA TGGGCTTCGA CAAGCAGGAT CTTCCGCCGC CCTCTTTCGA GGCAGCCATT 
GCCACGGGCG CCATCAAACT GGACGATCTC ACGCCCTCCG AACTGATTGG CATCTATGAT 
GCTCTGTTTT CCTGCCTGGT GTCCTGGCTG GAGGGCAACT CCATGGACCA GGTTCTATTC 
ACCTGCCTCT ACCTGCACGC TCCCGCACAG ATCAAGGATA AGGCGCTGCG CGTCTTCTGC 
ACTGCCGTGC GCAATCTCAT CGTGGTCATC AAGAAAATTA TTGCCGTGGC AGCTGTCAAC 
GAGGAGGAGG ACTTCCAGCT GTACGGAAAT TCTGCTCTTC TGGCTGCAGA GAAGGCACAG 
CCAGCCACTG TTTATAGCTC GCTAAAGGAT GTGGAGGATG AGCTCATACG CAAGTGCAAA 
AAGCTGACTT CCACGGAGGA CTGGATGGCC GTGGTTCACC GGCTGCGTTT CATGCGCCAT 
CTTTTCCAGG TGATCTACCA CGTGGAGCAA ATGGCCAGCA ATGATACCGT CGATGACAAG 
GTAGACATAT ACAAAATCCT GCTTGTGGCC TCCGAGATGC TTCCAGGAAT AAGGAACACT 
TTGGATCGGG GCACACAACC AGAGAAGGGG TCTGACGCTC CCAATCCCAT GGGTTTCTCT 
CCGCGCATCC ACGACCGCAG CCAACCGCCC GCGTTTCCGC GTAGCATTAA GATCAGGGAT 
CGTCCATCCA GTTATCAGTT TCTAGAGGAA ATGATTTCGC GCTTCAAATA CGCCTGCAAA 
GTCACCAAGT ACAAGGATTA CTATTCGGCG CTGAACTTCT TTATCGAGTA CAGCAAAAAG 
TCGGGCCAGT GCATCCTGTC CAGAAGCGTG CTGCAAACCC TGTTCAGCGC CAACATGCGT 
ATGGCGCACG GAAAGCTTCC CATGAAGCAG TTCCTGCGCC ACTCGGTTCA GGTCTTCAAC 
TCGCCACCCG TATTGAATGC CAAGCATCCG GTGGCTGCCG ATCCCAAGGT GCAGCAGCAC 
CTGGAGAACT TCTTCCGTTA CTGCATTAAC ATGAACACCT TCACGCAGTT CATTCGCATC 
TGCGGCTTCA ATCGCGCCAG ACAGCGGGAT AAGCTGGCGC GTCTGATAGA GAACTTCGAC 
ACCATCCAAG TGGATGCAGC GCGATTGGAC TCTATGATGA ACCAACTGGC CAACGAACGA 
GCCATGGAGG GCAACGAGCC CATGGCCACC GCTCTGAAGC ACAGCACCCA CTTCTCCACA 
TGGGTGCTGT ACAACTGTTT CCGTGCGATG CTGATCTTCC TGATGTCCGG CTTTGAGCTG 
GAACTATATG CTGTGCATGA GTTCCTCTAC ATCTACTGGT ATCCATACGA GTTCCTCATT 
GGCTTCCTGG TCTCCGCGCT GACGCGCACG GAGAACATCC TCCTGGCCCA GGAGGAGTAC 
GCCGAGCACC AAAGTAAGAC CCAGTCGGGC GGCAGCGGAG CGGCTAAAAA CCGCAAGGCG 
GCCAAGCCGA AAAAGAACAA AAAGACTCAA AGGCCGTACC GCGCCGAGAT CGTCTTCTAC 
CACGCGCTAC TCAGCCTCTG CGGCGGAATG TATAAAGCAA TGGGCGCCCT GACCAAAGAC 
GGACGCGTGC GACTGCCGCT GTCCAAGTTC GACAACGAGG AGATCCGCTA CAACCGACGC 
TTCCTGCCCT TCGCCACACT CACCAGTCCT CCGCCAGTTT CGTACGCAGA GTTCAAAAAC 
GTCCGGGAGC ACATGATGCG ACATAGCGTC GAGGATCTAT ATACCTATGC GGCCAAGCAC 
TTTGACCAGG CCCGCAATGT TCTCGAAAGC ATTCAAAACC CAGATCAGGA GATGCTGGAC 
CTCCTGCAAA TCGCACGGAC TAACTTTGTG GTGATGAATG TGCTGGCTCG CGGTCACCAG 
AAGGAAGTCA AGCGGCAGCC AGAGTTCGAC TTCTCCAAAC ACAGCTACTT CCCCATCATC 
AAGCTGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014546.1
CDS	138..2489
Translation	MYPSTLPAEADPSTMNGSVAEPPDFQAATAAAAAAARASSLEQDEWSVGECGFLDPDVQRTMRSGSAAEDITQYPMHGWVDVTKEFHDACAELQPGELAQDMLFGLFEAMSAIEIMDPKMDVGMGFDKQDLPPPSFEAAIATGAIKLDDLTPSELIGIYDALFSCLVSWLEGNSMDQVLFTCLYLHAPAQIKDKALRVFCTAVRNLIVVIKKIIAVAAVNEEEDFQLYGNSALLAAEKAQPATVYSSLKDVEDELIRKCKKLTSTEDWMAVVHRLRFMRHLFQVIYHVEQMASNDTVDDKVDIYKILLVASEMLPGIRNTLDRGTQPEKGSDAPNPMGFSPRIHDRSQPPAFPRSIKIRDRPSSYQFLEEMISRFKYACKVTKYKDYYSALNFFIEYSKKSGQCILSRSVLQTLFSANMRMAHGKLPMKQFLRHSVQVFNSPPVLNAKHPVAADPKVQQHLENFFRYCINMNTFTQFIRICGFNRARQRDKLARLIENFDTIQVDAARLDSMMNQLANERAMEGNEPMATALKHSTHFSTWVLYNCFRAMLIFLMSGFELELYAVHEFLYIYWYPYEFLIGFLVSALTRTENILLAQEEYAEHQSKTQSGGSGAAKNRKAAKPKKNKKTQRPYRAEIVFYHALLSLCGGMYKAMGALTKDGRVRLPLSKFDNEEIRYNRRFLPFATLTSPPPVSYAEFKNVREHMMRHSVEDLYTYAAKHFDQARNVLESIQNPDQEMLDLLQIARTNFVVMNVLARGHQKEVKRQPEFDFSKHSYFPIIKLK

Target ORF information:

RefSeq Version	NM_001014546.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster N(alpha)-acetyltransferase 35 (Naa35), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014546.2

ORF Insert Sequence:

ATGTATCCCT CGACCTTGCC GGCGGAGGCA GATCCGAGCA CCATGAACGG AAGCGTTGCA 
GAGCCTCCGG ACTTCCAGGC TGCCACGGCG GCCGCGGCGG CAGCTGCCCG GGCCAGTAGC 
TTGGAACAGG ACGAGTGGAG CGTGGGCGAG TGCGGATTTC TGGATCCGGA TGTGCAGCGC 
ACCATGCGGA GCGGCAGTGC TGCGGAGGAC ATCACCCAGT ACCCGATGCA CGGCTGGGTG 
GACGTAACCA AGGAGTTCCA CGATGCCTGC GCGGAGCTGC AGCCCGGGGA ACTGGCCCAG 
GATATGCTGT TCGGTCTTTT TGAGGCCATG TCAGCCATCG AGATCATGGA CCCCAAGATG 
GACGTGGGCA TGGGCTTCGA CAAGCAGGAT CTTCCGCCGC CCTCTTTCGA GGCAGCCATT 
GCCACGGGCG CCATCAAACT GGACGATCTC ACGCCCTCCG AACTGATTGG CATCTATGAT 
GCTCTGTTTT CCTGCCTGGT GTCCTGGCTG GAGGGCAACT CCATGGACCA GGTTCTATTC 
ACCTGCCTCT ACCTGCACGC TCCCGCACAG ATCAAGGATA AGGCGCTGCG CGTCTTCTGC 
ACTGCCGTGC GCAATCTCAT CGTGGTCATC AAGAAAATTA TTGCCGTGGC AGCTGTCAAC 
GAGGAGGAGG ACTTCCAGCT GTACGGAAAT TCTGCTCTTC TGGCTGCAGA GAAGGCACAG 
CCAGCCACTG TTTATAGCTC GCTAAAGGAT GTGGAGGATG AGCTCATACG CAAGTGCAAA 
AAGCTGACTT CCACGGAGGA CTGGATGGCC GTGGTTCACC GGCTGCGTTT CATGCGCCAT 
CTTTTCCAGG TGATCTACCA CGTGGAGCAA ATGGCCAGCA ATGATACCGT CGATGACAAG 
GTAGACATAT ACAAAATCCT GCTTGTGGCC TCCGAGATGC TTCCAGGAAT AAGGAACACT 
TTGGATCGGG GCACACAACC AGAGAAGGGG TCTGACGCTC CCAATCCCAT GGGTTTCTCT 
CCGCGCATCC ACGACCGCAG CCAACCGCCC GCGTTTCCGC GTAGCATTAA GATCAGGGAT 
CGTCCATCCA GTTATCAGTT TCTAGAGGAA ATGATTTCGC GCTTCAAATA CGCCTGCAAA 
GTCACCAAGT ACAAGGATTA CTATTCGGCG CTGAACTTCT TTATCGAGTA CAGCAAAAAG 
TCGGGCCAGT GCATCCTGTC CAGAAGCGTG CTGCAAACCC TGTTCAGCGC CAACATGCGT 
ATGGCGCACG GAAAGCTTCC CATGAAGCAG TTCCTGCGCC ACTCGGTTCA GGTCTTCAAC 
TCGCCACCCG TATTGAATGC CAAGCATCCG GTGGCTGCCG ATCCCAAGGT GCAGCAGCAC 
CTGGAGAACT TCTTCCGTTA CTGCATTAAC ATGAACACCT TCACGCAGTT CATTCGCATC 
TGCGGCTTCA ATCGCGCCAG ACAGCGGGAT AAGCTGGCGC GTCTGATAGA GAACTTCGAC 
ACCATCCAAG TGGATGCAGC GCGATTGGAC TCTATGATGA ACCAACTGGC CAACGAACGA 
GCCATGGAGG GCAACGAGCC CATGGCCACC GCTCTGAAGC ACAGCACCCA CTTCTCCACA 
TGGGTGCTGT ACAACTGTTT CCGTGCGATG CTGATCTTCC TGATGTCCGG CTTTGAGCTG 
GAACTATATG CTGTGCATGA GTTCCTCTAC ATCTACTGGT ATCCATACGA GTTCCTCATT 
GGCTTCCTGG TCTCCGCGCT GACGCGCACG GAGAACATCC TCCTGGCCCA GGAGGAGTAC 
GCCGAGCACC AAAGTAAGAC CCAGTCGGGC GGCAGCGGAG CGGCTAAAAA CCGCAAGGCG 
GCCAAGCCGA AAAAGAACAA AAAGACTCAA AGGCCGTACC GCGCCGAGAT CGTCTTCTAC 
CACGCGCTAC TCAGCCTCTG CGGCGGAATG TATAAAGCAA TGGGCGCCCT GACCAAAGAC 
GGACGCGTGC GACTGCCGCT GTCCAAGTTC GACAACGAGG AGATCCGCTA CAACCGACGC 
TTCCTGCCCT TCGCCACACT CACCAGTCCT CCGCCAGTTT CGTACGCAGA GTTCAAAAAC 
GTCCGGGAGC ACATGATGCG ACATAGCGTC GAGGATCTAT ATACCTATGC GGCCAAGCAC 
TTTGACCAGG CCCGCAATGT TCTCGAAAGC ATTCAAAACC CAGATCAGGA GATGCTGGAC 
CTCCTGCAAA TCGCACGGAC TAACTTTGTG GTGATGAATG TGCTGGCTCG CGGTCACCAG 
AAGGAAGTCA AGCGGCAGCC AGAGTTCGAC TTCTCCAAAC ACAGCTACTT CCCCATCATC 
AAGCTGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Naa35 ( NM_138047.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611891.1 Drosophila melanogaster N(alpha)-acetyltransferase 35 (Naa35), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa11133
Clone ID Related Accession (Same CDS sequence)	NM_138047.3
Accession Version	NM_138047.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	N(alpha)-acetyltransferase 35, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_138047.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTATCCCT CGACCTTGCC GGCGGAGGCA GATCCGAGCA CCATGAACGG AAGCAGCGTT 
GCAGAGCCTC CGGACTTCCA GGCTGCCACG GCGGCCGCGG CGGCAGCTGC CCGGGCCAGT 
AGCTTGGAAC AGGACGAGTG GAGCGTGGGC GAGTGCGGAT TTCTGGATCC GGATGTGCAG 
CGCACCATGC GGAGCGGCAG TGCTGCGGAG GACATCACCC AGTACCCGAT GCACGGCTGG 
GTGGACGTAA CCAAGGAGTT CCACGATGCC TGCGCGGAGC TGCAGCCCGG GGAACTGGCC 
CAGGATATGC TGTTCGGTCT TTTTGAGGCC ATGTCAGCCA TCGAGATCAT GGACCCCAAG 
ATGGACGTGG GCATGGGCTT CGACAAGCAG GATCTTCCGC CGCCCTCTTT CGAGGCAGCC 
ATTGCCACGG GCGCCATCAA ACTGGACGAT CTCACGCCCT CCGAACTGAT TGGCATCTAT 
GATGCTCTGT TTTCCTGCCT GGTGTCCTGG CTGGAGGGCA ACTCCATGGA CCAGGTTCTA 
TTCACCTGCC TCTACCTGCA CGCTCCCGCA CAGATCAAGG ATAAGGCGCT GCGCGTCTTC 
TGCACTGCCG TGCGCAATCT CATCGTGGTC ATCAAGAAAA TTATTGCCGT GGCAGCTGTC 
AACGAGGAGG AGGACTTCCA GCTGTACGGA AATTCTGCTC TTCTGGCTGC AGAGAAGGCA 
CAGCCAGCCA CTGTTTATAG CTCGCTAAAG GATGTGGAGG ATGAGCTCAT ACGCAAGTGC 
AAAAAGCTGA CTTCCACGGA GGACTGGATG GCCGTGGTTC ACCGGCTGCG TTTCATGCGC 
CATCTTTTCC AGGTGATCTA CCACGTGGAG CAAATGGCCA GCAATGATAC CGTCGATGAC 
AAGGTAGACA TATACAAAAT CCTGCTTGTG GCCTCCGAGA TGCTTCCAGG AATAAGGAAC 
ACTTTGGATC GGGGCACACA ACCAGAGAAG GGGTCTGACG CTCCCAATCC CATGGGTTTC 
TCTCCGCGCA TCCACGACCG CAGCCAACCG CCCGCGTTTC CGCGTAGCAT TAAGATCAGG 
GATCGTCCAT CCAGTTATCA GTTTCTAGAG GAAATGATTT CGCGCTTCAA ATACGCCTGC 
AAAGTCACCA AGTACAAGGA TTACTATTCG GCGCTGAACT TCTTTATCGA GTACAGCAAA 
AAGTCGGGCC AGTGCATCCT GTCCAGAAGC GTGCTGCAAA CCCTGTTCAG CGCCAACATG 
CGTATGGCGC ACGGAAAGCT TCCCATGAAG CAGTTCCTGC GCCACTCGGT TCAGGTCTTC 
AACTCGCCAC CCGTATTGAA TGCCAAGCAT CCGGTGGCTG CCGATCCCAA GGTGCAGCAG 
CACCTGGAGA ACTTCTTCCG TTACTGCATT AACATGAACA CCTTCACGCA GTTCATTCGC 
ATCTGCGGCT TCAATCGCGC CAGACAGCGG GATAAGCTGG CGCGTCTGAT AGAGAACTTC 
GACACCATCC AAGTGGATGC AGCGCGATTG GACTCTATGA TGAACCAACT GGCCAACGAA 
CGAGCCATGG AGGGCAACGA GCCCATGGCC ACCGCTCTGA AGCACAGCAC CCACTTCTCC 
ACATGGGTGC TGTACAACTG TTTCCGTGCG ATGCTGATCT TCCTGATGTC CGGCTTTGAG 
CTGGAACTAT ATGCTGTGCA TGAGTTCCTC TACATCTACT GGTATCCATA CGAGTTCCTC 
ATTGGCTTCC TGGTCTCCGC GCTGACGCGC ACGGAGAACA TCCTCCTGGC CCAGGAGGAG 
TACGCCGAGC ACCAAAGTAA GACCCAGTCG GGCGGCAGCG GAGCGGCTAA AAACCGCAAG 
GCGGCCAAGC CGAAAAAGAA CAAAAAGACT CAAAGGCCGT ACCGCGCCGA GATCGTCTTC 
TACCACGCGC TACTCAGCCT CTGCGGCGGA ATGTATAAAG CAATGGGCGC CCTGACCAAA 
GACGGACGCG TGCGACTGCC GCTGTCCAAG TTCGACAACG AGGAGATCCG CTACAACCGA 
CGCTTCCTGC CCTTCGCCAC ACTCACCAGT CCTCCGCCAG TTTCGTACGC AGAGTTCAAA 
AACGTCCGGG AGCACATGAT GCGACATAGC GTCGAGGATC TATATACCTA TGCGGCCAAG 
CACTTTGACC AGGCCCGCAA TGTTCTCGAA AGCATTCAAA ACCCAGATCA GGAGATGCTG 
GACCTCCTGC AAATCGCACG GACTAACTTT GTGGTGATGA ATGTGCTGGC TCGCGGTCAC 
CAGAAGGAAG TCAAGCGGCA GCCAGAGTTC GACTTCTCCA AACACAGCTA CTTCCCCATC 
ATCAAGCTGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611891.1
CDS	138..2492
Translation	MYPSTLPAEADPSTMNGSSVAEPPDFQAATAAAAAAARASSLEQDEWSVGECGFLDPDVQRTMRSGSAAEDITQYPMHGWVDVTKEFHDACAELQPGELAQDMLFGLFEAMSAIEIMDPKMDVGMGFDKQDLPPPSFEAAIATGAIKLDDLTPSELIGIYDALFSCLVSWLEGNSMDQVLFTCLYLHAPAQIKDKALRVFCTAVRNLIVVIKKIIAVAAVNEEEDFQLYGNSALLAAEKAQPATVYSSLKDVEDELIRKCKKLTSTEDWMAVVHRLRFMRHLFQVIYHVEQMASNDTVDDKVDIYKILLVASEMLPGIRNTLDRGTQPEKGSDAPNPMGFSPRIHDRSQPPAFPRSIKIRDRPSSYQFLEEMISRFKYACKVTKYKDYYSALNFFIEYSKKSGQCILSRSVLQTLFSANMRMAHGKLPMKQFLRHSVQVFNSPPVLNAKHPVAADPKVQQHLENFFRYCINMNTFTQFIRICGFNRARQRDKLARLIENFDTIQVDAARLDSMMNQLANERAMEGNEPMATALKHSTHFSTWVLYNCFRAMLIFLMSGFELELYAVHEFLYIYWYPYEFLIGFLVSALTRTENILLAQEEYAEHQSKTQSGGSGAAKNRKAAKPKKNKKTQRPYRAEIVFYHALLSLCGGMYKAMGALTKDGRVRLPLSKFDNEEIRYNRRFLPFATLTSPPPVSYAEFKNVREHMMRHSVEDLYTYAAKHFDQARNVLESIQNPDQEMLDLLQIARTNFVVMNVLARGHQKEVKRQPEFDFSKHSYFPIIKLK

Target ORF information:

RefSeq Version	NM_138047.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster N(alpha)-acetyltransferase 35 (Naa35), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138047.3

ORF Insert Sequence:

ATGTATCCCT CGACCTTGCC GGCGGAGGCA GATCCGAGCA CCATGAACGG AAGCAGCGTT 
GCAGAGCCTC CGGACTTCCA GGCTGCCACG GCGGCCGCGG CGGCAGCTGC CCGGGCCAGT 
AGCTTGGAAC AGGACGAGTG GAGCGTGGGC GAGTGCGGAT TTCTGGATCC GGATGTGCAG 
CGCACCATGC GGAGCGGCAG TGCTGCGGAG GACATCACCC AGTACCCGAT GCACGGCTGG 
GTGGACGTAA CCAAGGAGTT CCACGATGCC TGCGCGGAGC TGCAGCCCGG GGAACTGGCC 
CAGGATATGC TGTTCGGTCT TTTTGAGGCC ATGTCAGCCA TCGAGATCAT GGACCCCAAG 
ATGGACGTGG GCATGGGCTT CGACAAGCAG GATCTTCCGC CGCCCTCTTT CGAGGCAGCC 
ATTGCCACGG GCGCCATCAA ACTGGACGAT CTCACGCCCT CCGAACTGAT TGGCATCTAT 
GATGCTCTGT TTTCCTGCCT GGTGTCCTGG CTGGAGGGCA ACTCCATGGA CCAGGTTCTA 
TTCACCTGCC TCTACCTGCA CGCTCCCGCA CAGATCAAGG ATAAGGCGCT GCGCGTCTTC 
TGCACTGCCG TGCGCAATCT CATCGTGGTC ATCAAGAAAA TTATTGCCGT GGCAGCTGTC 
AACGAGGAGG AGGACTTCCA GCTGTACGGA AATTCTGCTC TTCTGGCTGC AGAGAAGGCA 
CAGCCAGCCA CTGTTTATAG CTCGCTAAAG GATGTGGAGG ATGAGCTCAT ACGCAAGTGC 
AAAAAGCTGA CTTCCACGGA GGACTGGATG GCCGTGGTTC ACCGGCTGCG TTTCATGCGC 
CATCTTTTCC AGGTGATCTA CCACGTGGAG CAAATGGCCA GCAATGATAC CGTCGATGAC 
AAGGTAGACA TATACAAAAT CCTGCTTGTG GCCTCCGAGA TGCTTCCAGG AATAAGGAAC 
ACTTTGGATC GGGGCACACA ACCAGAGAAG GGGTCTGACG CTCCCAATCC CATGGGTTTC 
TCTCCGCGCA TCCACGACCG CAGCCAACCG CCCGCGTTTC CGCGTAGCAT TAAGATCAGG 
GATCGTCCAT CCAGTTATCA GTTTCTAGAG GAAATGATTT CGCGCTTCAA ATACGCCTGC 
AAAGTCACCA AGTACAAGGA TTACTATTCG GCGCTGAACT TCTTTATCGA GTACAGCAAA 
AAGTCGGGCC AGTGCATCCT GTCCAGAAGC GTGCTGCAAA CCCTGTTCAG CGCCAACATG 
CGTATGGCGC ACGGAAAGCT TCCCATGAAG CAGTTCCTGC GCCACTCGGT TCAGGTCTTC 
AACTCGCCAC CCGTATTGAA TGCCAAGCAT CCGGTGGCTG CCGATCCCAA GGTGCAGCAG 
CACCTGGAGA ACTTCTTCCG TTACTGCATT AACATGAACA CCTTCACGCA GTTCATTCGC 
ATCTGCGGCT TCAATCGCGC CAGACAGCGG GATAAGCTGG CGCGTCTGAT AGAGAACTTC 
GACACCATCC AAGTGGATGC AGCGCGATTG GACTCTATGA TGAACCAACT GGCCAACGAA 
CGAGCCATGG AGGGCAACGA GCCCATGGCC ACCGCTCTGA AGCACAGCAC CCACTTCTCC 
ACATGGGTGC TGTACAACTG TTTCCGTGCG ATGCTGATCT TCCTGATGTC CGGCTTTGAG 
CTGGAACTAT ATGCTGTGCA TGAGTTCCTC TACATCTACT GGTATCCATA CGAGTTCCTC 
ATTGGCTTCC TGGTCTCCGC GCTGACGCGC ACGGAGAACA TCCTCCTGGC CCAGGAGGAG 
TACGCCGAGC ACCAAAGTAA GACCCAGTCG GGCGGCAGCG GAGCGGCTAA AAACCGCAAG 
GCGGCCAAGC CGAAAAAGAA CAAAAAGACT CAAAGGCCGT ACCGCGCCGA GATCGTCTTC 
TACCACGCGC TACTCAGCCT CTGCGGCGGA ATGTATAAAG CAATGGGCGC CCTGACCAAA 
GACGGACGCG TGCGACTGCC GCTGTCCAAG TTCGACAACG AGGAGATCCG CTACAACCGA 
CGCTTCCTGC CCTTCGCCAC ACTCACCAGT CCTCCGCCAG TTTCGTACGC AGAGTTCAAA 
AACGTCCGGG AGCACATGAT GCGACATAGC GTCGAGGATC TATATACCTA TGCGGCCAAG 
CACTTTGACC AGGCCCGCAA TGTTCTCGAA AGCATTCAAA ACCCAGATCA GGAGATGCTG 
GACCTCCTGC AAATCGCACG GACTAACTTT GTGGTGATGA ATGTGCTGGC TCGCGGTCAC 
CAGAAGGAAG TCAAGCGGCA GCCAGAGTTC GACTTCTCCA AACACAGCTA CTTCCCCATC 
ATCAAGCTGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Naa35 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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