yki cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	yki
Entrez Gene ID	37851
Full Name	yorkie
Synonyms	CG4005,Dmel\CG4005,YAP,YKI,Yki,yap
General protein information	Preferred Names yorkie Names CG4005 gene product from transcript CG4005-RG CG4005-PD CG4005-PF CG4005-PG yki-PD yki-PF yki-PG yorki
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 60B7-60B8

mRNA and Protein(s)

mRNA	Protein	Name
NM_138035.3	NP_611879.4	yorkie, isoform D
NM_001043103.2	NP_001036568.2	yorkie, isoform F
NM_166657.5	NP_726414.3	yorkie, isoform G
NM_001363931.1	NP_001350857.1	yorkie, isoform H

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	yki	NP_726414.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006045	XP_556728.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

found that human E2F1 competes with YAP for TEAD1 binding, affecting YAP activity, indicating that this mode of cross-regulation is conserved
Title: A Balance of Yki/Sd Activator and E2F1/Sd Repressor Complexes Controls Cell Survival and Affects Organ Size.

Data show that Yorkie (Yki) and Scalloped (Sd) are required for epidermal wound closure in the larval epidermis.
Title: Yorkie regulates epidermal wound healing in Drosophila larvae independently of cell proliferation and apoptosis.

In ovary escort cells (ECs) both Hh and Yki limit production of BMP ligands to allow germline differentiation. Loss of Yki produced a more severe germarial phenotype than loss of Hh signaling and principally induced a different BMP ligand. Moreover, Yki activity reporters and epistasis tests showed that Yki does not mediate the key actions of Hh signaling in ECs.
Title: Yorkie and Hedgehog independently restrict BMP production in escort cells to permit germline differentiation in the

We demonstrate the efficacy of the MARGE technique by flip-out (clonal and disc-wide) of a Ubi-GFP-carrying construct in imaginal discs, and by inducing a known yki mutant phenotype in the Drosophila ovary
Title: Mutant analysis by rescue gene excision: New tools for mosaic studies in Drosophila.

aken together, these results reveal that inactivation of Rpd3 independently regulates JNK and Yki activities and that both Hippo and JNK signaling pathways contribute to Rpd3 RNAi-induced apoptosis.
Title: Loss of histone deacetylase HDAC1 induces cell death in Drosophila epithelial cells through JNK and Hippo signaling.

The following yki gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the yki cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa11115	NM_138035.3 Latest version!	Drosophila melanogaster yorkie (yki), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa11116	NM_166657.5 Latest version!	Drosophila melanogaster yorkie (yki), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa11116	NM_001043103.2 Latest version!	Drosophila melanogaster yorkie (yki), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa57980	NM_001363931.1 Latest version!	Drosophila melanogaster yorkie (yki), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

yki ( NM_138035.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611879.4 Drosophila melanogaster yorkie (yki), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa11115
Clone ID Related Accession (Same CDS sequence)	NM_138035.3
Accession Version	NM_138035.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	yorkie, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Dec 17, 2009 this sequence version replaced NM_138035.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTAACGA CGATGTCAGC CAGCAGCAAT ACAAACAGCC TGATCGAGAA GGAGATCGAC 
GACGAGGACA TGCTTTCGCC GATCAAGTCC AACAACCTGG TGGTGCGGGT CAACCAGGAC 
ACGGACGACA ACCTGCAGGC GCTATTCGAC AGCGTCCTGA ATCCGGGTGA CGCCAAGCGC 
CCGCTGCAGC TGCCCCTGCG CATGCGGAAG CTGCCCAACT CCTTCTTCAC GCCCCCGGCG 
CCCTCGCACT CGCGGGCCAA CAGCGCCGAC TCCACCTACG ACGCGGGCTC CCAGTCGAGC 
ATCAACATCG GGAACAAGGC GTCCATCGTC CAGCAGCCAG ATGGCCAGTC GCCCATCGCC 
GCCATCCCCC AGCTCCAGAT TCAGCCGTCT CCCCAGCACA GCCGCCTGGC GATACATCAC 
TCCCGAGCCC GCAGCAGCCC CGCCTCGCTG CAGCAGAACT ACAATGTGCG CGCCCGGAGC 
GACGCAGCAG CAGCCAACAA TCCGAATGCC AATCCGAGCA GCCAACAGCA GCCCGCTGGG 
CCCACTTTCC CAGAGAACAG TGCCCAAGAG TTCCCCAGCG GCGCCCCGGC CAGCTCGGCC 
ATTGATCTGG ACGCCATGAA CACCTGCATG TCGCAGGACA TTCCCATGTC CATGCAGACA 
GTGCACAAGA AGCAGCGCTC CTACGACGTC ATCAGCCCCA TTCAGTTGAA CCGCCAACTA 
GGCGCCTTGC CGCCGGGATG GGAGCAAGCC AAGACCAATG ATGGCCAGAT CTACTACTTG 
AATCATACTA CAAAATCTAC GCAGTGGGAG GATCCCAGAA TCCAATATCG CCAGCAGCAG 
CAAATCTTGA TGGCCGAGCG AATAAAGCAG AATGAATCTG GGCTTAGCGT GCTCGACTGC 
CCAGATAACT TAGTGTCTTC CCTCCAGATT GAGGATAATC TTTGCAGTAA CTTGTTCAAT 
GACGCACAGG CCATTGTAAA TCCGCCGTCT TCCCACAAAC CTGACGATTT GGAATGGTAT 
AAAATTAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611879.4
CDS	240..1271
Translation	MLTTMSASSNTNSLIEKEIDDEDMLSPIKSNNLVVRVNQDTDDNLQALFDSVLNPGDAKRPLQLPLRMRKLPNSFFTPPAPSHSRANSADSTYDAGSQSSINIGNKASIVQQPDGQSPIAAIPQLQIQPSPQHSRLAIHHSRARSSPASLQQNYNVRARSDAAAANNPNANPSSQQQPAGPTFPENSAQEFPSGAPASSAIDLDAMNTCMSQDIPMSMQTVHKKQRSYDVISPIQLNRQLGALPPGWEQAKTNDGQIYYLNHTTKSTQWEDPRIQYRQQQQILMAERIKQNESGLSVLDCPDNLVSSLQIEDNLCSNLFNDAQAIVNPPSSHKPDDLEWYKIN

Target ORF information:

RefSeq Version	NM_138035.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster yorkie (yki), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138035.3

ORF Insert Sequence:

ATGTTAACGA CGATGTCAGC CAGCAGCAAT ACAAACAGCC TGATCGAGAA GGAGATCGAC 
GACGAGGACA TGCTTTCGCC GATCAAGTCC AACAACCTGG TGGTGCGGGT CAACCAGGAC 
ACGGACGACA ACCTGCAGGC GCTATTCGAC AGCGTCCTGA ATCCGGGTGA CGCCAAGCGC 
CCGCTGCAGC TGCCCCTGCG CATGCGGAAG CTGCCCAACT CCTTCTTCAC GCCCCCGGCG 
CCCTCGCACT CGCGGGCCAA CAGCGCCGAC TCCACCTACG ACGCGGGCTC CCAGTCGAGC 
ATCAACATCG GGAACAAGGC GTCCATCGTC CAGCAGCCAG ATGGCCAGTC GCCCATCGCC 
GCCATCCCCC AGCTCCAGAT TCAGCCGTCT CCCCAGCACA GCCGCCTGGC GATACATCAC 
TCCCGAGCCC GCAGCAGCCC CGCCTCGCTG CAGCAGAACT ACAATGTGCG CGCCCGGAGC 
GACGCAGCAG CAGCCAACAA TCCGAATGCC AATCCGAGCA GCCAACAGCA GCCCGCTGGG 
CCCACTTTCC CAGAGAACAG TGCCCAAGAG TTCCCCAGCG GCGCCCCGGC CAGCTCGGCC 
ATTGATCTGG ACGCCATGAA CACCTGCATG TCGCAGGACA TTCCCATGTC CATGCAGACA 
GTGCACAAGA AGCAGCGCTC CTACGACGTC ATCAGCCCCA TTCAGTTGAA CCGCCAACTA 
GGCGCCTTGC CGCCGGGATG GGAGCAAGCC AAGACCAATG ATGGCCAGAT CTACTACTTG 
AATCATACTA CAAAATCTAC GCAGTGGGAG GATCCCAGAA TCCAATATCG CCAGCAGCAG 
CAAATCTTGA TGGCCGAGCG AATAAAGCAG AATGAATCTG GGCTTAGCGT GCTCGACTGC 
CCAGATAACT TAGTGTCTTC CCTCCAGATT GAGGATAATC TTTGCAGTAA CTTGTTCAAT 
GACGCACAGG CCATTGTAAA TCCGCCGTCT TCCCACAAAC CTGACGATTT GGAATGGTAT 
AAAATTAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

yki ( NM_001043103.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036568.2 Drosophila melanogaster yorkie (yki), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa11116
Clone ID Related Accession (Same CDS sequence)	NM_001043103.2 , NM_166657.5
Accession Version	NM_001043103.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	yorkie, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Dec 17, 2009 this sequence version replaced NM_001043103.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTAACGA CGATGTCAGC CAGCAGCAAT ACAAACAGCC TGATCGAGAA GGAGATCGAC 
GACGAGGACA TGCTTTCGCC GATCAAGTCC AACAACCTGG TGGTGCGGGT CAACCAGGAC 
ACGGACGACA ACCTGCAGGC GCTATTCGAC AGCGTCCTGA ATCCGGGTGA CGCCAAGCGC 
CCGCTGCAGC TGCCCCTGCG CATGCGGAAG CTGCCCAACT CCTTCTTCAC GCCCCCGGCG 
CCCTCGCACT CGCGGGCCAA CAGCGCCGAC TCCACCTACG ACGCGGGCTC CCAGTCGAGC 
ATCAACATCG GGAACAAGGC GTCCATCGTC CAGCAGCCAG ATGGCCAGTC GCCCATCGCC 
GCCATCCCCC AGCTCCAGAT TCAGCCGTCT CCCCAGCACA GCCGCCTGGC GATACATCAC 
TCCCGAGCCC GCAGCAGCCC CGCCTCGCTG CAGCAGAACT ACAATGTGCG CGCCCGGAGC 
GACGCAGCAG CAGCCAACAA TCCGAATGCC AATCCGAGCA GCCAACAGCA GCCCGCTGGG 
CCCACTTTCC CAGAGAACAG TGCCCAAGAG TTCCCCAGCG GCGCCCCGGC CAGCTCGGCC 
ATTGATCTGG ACGCCATGAA CACCTGCATG TCGCAGGACA TTCCCATGTC CATGCAGACA 
GTGCACAAGA AGCAGCGCTC CTACGACGTC ATCAGCCCCA TTCAGTTGAA CCGCCAACTA 
GGCGCCTTGC CGCCGGGATG GGAGCAAGCC AAGACCAATG ATGGCCAGAT CTACTACTTG 
AATCATACTA CAAAATCTAC GCAGTGGGAG GATCCCAGAA TCCAATATCG CCAGCAGCAG 
CAAATCTTGA TGGCCGAGCG AATAAAGCAG AATGATGTTT TGCAAACTAC AAAACAAACT 
ACCACATCGA CCATTGCTAA CAATTTGGGT CCACTGCCGG ATGGTTGGGA GCAGGCAGTT 
ACCGAGTCCG GAGATCTTTA CTTTATAAAT CACATTGATC GAACGACTTC ATGGAATGAT 
CCCAGAATGC AATCTGGGCT TAGCGTGCTC GACTGCCCAG ATAACTTAGT GTCTTCCCTC 
CAGATTGAGG ATAATCTTTG CAGTAACTTG TTCAATGACG CACAGGCCAT TGTAAATCCG 
CCGTCTTCCC ACAAACCTGA CGATTTGGAA TGGTATAAAA TTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036568.2
CDS	240..1427
Translation	MLTTMSASSNTNSLIEKEIDDEDMLSPIKSNNLVVRVNQDTDDNLQALFDSVLNPGDAKRPLQLPLRMRKLPNSFFTPPAPSHSRANSADSTYDAGSQSSINIGNKASIVQQPDGQSPIAAIPQLQIQPSPQHSRLAIHHSRARSSPASLQQNYNVRARSDAAAANNPNANPSSQQQPAGPTFPENSAQEFPSGAPASSAIDLDAMNTCMSQDIPMSMQTVHKKQRSYDVISPIQLNRQLGALPPGWEQAKTNDGQIYYLNHTTKSTQWEDPRIQYRQQQQILMAERIKQNDVLQTTKQTTTSTIANNLGPLPDGWEQAVTESGDLYFINHIDRTTSWNDPRMQSGLSVLDCPDNLVSSLQIEDNLCSNLFNDAQAIVNPPSSHKPDDLEWYKIN

Target ORF information:

RefSeq Version	NM_001043103.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster yorkie (yki), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043103.2

ORF Insert Sequence:

ATGTTAACGA CGATGTCAGC CAGCAGCAAT ACAAACAGCC TGATCGAGAA GGAGATCGAC 
GACGAGGACA TGCTTTCGCC GATCAAGTCC AACAACCTGG TGGTGCGGGT CAACCAGGAC 
ACGGACGACA ACCTGCAGGC GCTATTCGAC AGCGTCCTGA ATCCGGGTGA CGCCAAGCGC 
CCGCTGCAGC TGCCCCTGCG CATGCGGAAG CTGCCCAACT CCTTCTTCAC GCCCCCGGCG 
CCCTCGCACT CGCGGGCCAA CAGCGCCGAC TCCACCTACG ACGCGGGCTC CCAGTCGAGC 
ATCAACATCG GGAACAAGGC GTCCATCGTC CAGCAGCCAG ATGGCCAGTC GCCCATCGCC 
GCCATCCCCC AGCTCCAGAT TCAGCCGTCT CCCCAGCACA GCCGCCTGGC GATACATCAC 
TCCCGAGCCC GCAGCAGCCC CGCCTCGCTG CAGCAGAACT ACAATGTGCG CGCCCGGAGC 
GACGCAGCAG CAGCCAACAA TCCGAATGCC AATCCGAGCA GCCAACAGCA GCCCGCTGGG 
CCCACTTTCC CAGAGAACAG TGCCCAAGAG TTCCCCAGCG GCGCCCCGGC CAGCTCGGCC 
ATTGATCTGG ACGCCATGAA CACCTGCATG TCGCAGGACA TTCCCATGTC CATGCAGACA 
GTGCACAAGA AGCAGCGCTC CTACGACGTC ATCAGCCCCA TTCAGTTGAA CCGCCAACTA 
GGCGCCTTGC CGCCGGGATG GGAGCAAGCC AAGACCAATG ATGGCCAGAT CTACTACTTG 
AATCATACTA CAAAATCTAC GCAGTGGGAG GATCCCAGAA TCCAATATCG CCAGCAGCAG 
CAAATCTTGA TGGCCGAGCG AATAAAGCAG AATGATGTTT TGCAAACTAC AAAACAAACT 
ACCACATCGA CCATTGCTAA CAATTTGGGT CCACTGCCGG ATGGTTGGGA GCAGGCAGTT 
ACCGAGTCCG GAGATCTTTA CTTTATAAAT CACATTGATC GAACGACTTC ATGGAATGAT 
CCCAGAATGC AATCTGGGCT TAGCGTGCTC GACTGCCCAG ATAACTTAGT GTCTTCCCTC 
CAGATTGAGG ATAATCTTTG CAGTAACTTG TTCAATGACG CACAGGCCAT TGTAAATCCG 
CCGTCTTCCC ACAAACCTGA CGATTTGGAA TGGTATAAAA TTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

yki ( NM_166657.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726414.3 Drosophila melanogaster yorkie (yki), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa11116
Clone ID Related Accession (Same CDS sequence)	NM_001043103.2 , NM_166657.5
Accession Version	NM_166657.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	yorkie, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166657.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTAACGA CGATGTCAGC CAGCAGCAAT ACAAACAGCC TGATCGAGAA GGAGATCGAC 
GACGAGGACA TGCTTTCGCC GATCAAGTCC AACAACCTGG TGGTGCGGGT CAACCAGGAC 
ACGGACGACA ACCTGCAGGC GCTATTCGAC AGCGTCCTGA ATCCGGGTGA CGCCAAGCGC 
CCGCTGCAGC TGCCCCTGCG CATGCGGAAG CTGCCCAACT CCTTCTTCAC GCCCCCGGCG 
CCCTCGCACT CGCGGGCCAA CAGCGCCGAC TCCACCTACG ACGCGGGCTC CCAGTCGAGC 
ATCAACATCG GGAACAAGGC GTCCATCGTC CAGCAGCCAG ATGGCCAGTC GCCCATCGCC 
GCCATCCCCC AGCTCCAGAT TCAGCCGTCT CCCCAGCACA GCCGCCTGGC GATACATCAC 
TCCCGAGCCC GCAGCAGCCC CGCCTCGCTG CAGCAGAACT ACAATGTGCG CGCCCGGAGC 
GACGCAGCAG CAGCCAACAA TCCGAATGCC AATCCGAGCA GCCAACAGCA GCCCGCTGGG 
CCCACTTTCC CAGAGAACAG TGCCCAAGAG TTCCCCAGCG GCGCCCCGGC CAGCTCGGCC 
ATTGATCTGG ACGCCATGAA CACCTGCATG TCGCAGGACA TTCCCATGTC CATGCAGACA 
GTGCACAAGA AGCAGCGCTC CTACGACGTC ATCAGCCCCA TTCAGTTGAA CCGCCAACTA 
GGCGCCTTGC CGCCGGGATG GGAGCAAGCC AAGACCAATG ATGGCCAGAT CTACTACTTG 
AATCATACTA CAAAATCTAC GCAGTGGGAG GATCCCAGAA TCCAATATCG CCAGCAGCAG 
CAAATCTTGA TGGCCGAGCG AATAAAGCAG AATGATGTTT TGCAAACTAC AAAACAAACT 
ACCACATCGA CCATTGCTAA CAATTTGGGT CCACTGCCGG ATGGTTGGGA GCAGGCAGTT 
ACCGAGTCCG GAGATCTTTA CTTTATAAAT CACATTGATC GAACGACTTC ATGGAATGAT 
CCCAGAATGC AATCTGGGCT TAGCGTGCTC GACTGCCCAG ATAACTTAGT GTCTTCCCTC 
CAGATTGAGG ATAATCTTTG CAGTAACTTG TTCAATGACG CACAGGCCAT TGTAAATCCG 
CCGTCTTCCC ACAAACCTGA CGATTTGGAA TGGTATAAAA TTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726414.3
CDS	175..1362
Translation	MLTTMSASSNTNSLIEKEIDDEDMLSPIKSNNLVVRVNQDTDDNLQALFDSVLNPGDAKRPLQLPLRMRKLPNSFFTPPAPSHSRANSADSTYDAGSQSSINIGNKASIVQQPDGQSPIAAIPQLQIQPSPQHSRLAIHHSRARSSPASLQQNYNVRARSDAAAANNPNANPSSQQQPAGPTFPENSAQEFPSGAPASSAIDLDAMNTCMSQDIPMSMQTVHKKQRSYDVISPIQLNRQLGALPPGWEQAKTNDGQIYYLNHTTKSTQWEDPRIQYRQQQQILMAERIKQNDVLQTTKQTTTSTIANNLGPLPDGWEQAVTESGDLYFINHIDRTTSWNDPRMQSGLSVLDCPDNLVSSLQIEDNLCSNLFNDAQAIVNPPSSHKPDDLEWYKIN

Target ORF information:

RefSeq Version	NM_166657.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster yorkie (yki), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166657.5

ORF Insert Sequence:

ATGTTAACGA CGATGTCAGC CAGCAGCAAT ACAAACAGCC TGATCGAGAA GGAGATCGAC 
GACGAGGACA TGCTTTCGCC GATCAAGTCC AACAACCTGG TGGTGCGGGT CAACCAGGAC 
ACGGACGACA ACCTGCAGGC GCTATTCGAC AGCGTCCTGA ATCCGGGTGA CGCCAAGCGC 
CCGCTGCAGC TGCCCCTGCG CATGCGGAAG CTGCCCAACT CCTTCTTCAC GCCCCCGGCG 
CCCTCGCACT CGCGGGCCAA CAGCGCCGAC TCCACCTACG ACGCGGGCTC CCAGTCGAGC 
ATCAACATCG GGAACAAGGC GTCCATCGTC CAGCAGCCAG ATGGCCAGTC GCCCATCGCC 
GCCATCCCCC AGCTCCAGAT TCAGCCGTCT CCCCAGCACA GCCGCCTGGC GATACATCAC 
TCCCGAGCCC GCAGCAGCCC CGCCTCGCTG CAGCAGAACT ACAATGTGCG CGCCCGGAGC 
GACGCAGCAG CAGCCAACAA TCCGAATGCC AATCCGAGCA GCCAACAGCA GCCCGCTGGG 
CCCACTTTCC CAGAGAACAG TGCCCAAGAG TTCCCCAGCG GCGCCCCGGC CAGCTCGGCC 
ATTGATCTGG ACGCCATGAA CACCTGCATG TCGCAGGACA TTCCCATGTC CATGCAGACA 
GTGCACAAGA AGCAGCGCTC CTACGACGTC ATCAGCCCCA TTCAGTTGAA CCGCCAACTA 
GGCGCCTTGC CGCCGGGATG GGAGCAAGCC AAGACCAATG ATGGCCAGAT CTACTACTTG 
AATCATACTA CAAAATCTAC GCAGTGGGAG GATCCCAGAA TCCAATATCG CCAGCAGCAG 
CAAATCTTGA TGGCCGAGCG AATAAAGCAG AATGATGTTT TGCAAACTAC AAAACAAACT 
ACCACATCGA CCATTGCTAA CAATTTGGGT CCACTGCCGG ATGGTTGGGA GCAGGCAGTT 
ACCGAGTCCG GAGATCTTTA CTTTATAAAT CACATTGATC GAACGACTTC ATGGAATGAT 
CCCAGAATGC AATCTGGGCT TAGCGTGCTC GACTGCCCAG ATAACTTAGT GTCTTCCCTC 
CAGATTGAGG ATAATCTTTG CAGTAACTTG TTCAATGACG CACAGGCCAT TGTAAATCCG 
CCGTCTTCCC ACAAACCTGA CGATTTGGAA TGGTATAAAA TTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

yki ( NM_001363931.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001350857.1 Drosophila melanogaster yorkie (yki), transcript variant H, mRNA.

Price & Availability↑

CloneID	OFa57980
Clone ID Related Accession (Same CDS sequence)	NM_001363931.1
Accession Version	NM_001363931.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	yorkie, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGCGCGT GCCTAATCGC TAAGATAATT CTATGTAGTT TTCGTTTGTA TACAATAAGT 
GCCTTTTATA TGTTAACGAC GATGTCAGCC AGCAGCAATA CAAACAGCCT GATCGAGAAG 
GAGATCGACG ACGAGGACAT GCTTTCGCCG ATCAAGTCCA ACAACCTGGT GGTGCGGGTC 
AACCAGGACA CGGACGACAA CCTGCAGGCG CTATTCGACA GCGTCCTGAA TCCGGGTGAC 
GCCAAGCGCC CGCTGCAGCT GCCCCTGCGC ATGCGGAAGC TGCCCAACTC CTTCTTCACG 
CCCCCGGCGC CCTCGCACTC GCGGGCCAAC AGCGCCGACT CCACCTACGA CGCGGGCTCC 
CAGTCGAGCA TCAACATCGG GAACAAGGCG TCCATCGTCC AGCAGCCAGA TGGCCAGTCG 
CCCATCGCCG CCATCCCCCA GCTCCAGATT CAGCCGTCTC CCCAGCACAG CCGCCTGGCG 
ATACATCACT CCCGAGCCCG CAGCAGCCCC GCCTCGCTGC AGCAGAACTA CAATGTGCGC 
GCCCGGAGCG ACGCAGCAGC AGCCAACAAT CCGAATGCCA ATCCGAGCAG CCAACAGCAG 
CCCGCTGGGC CCACTTTCCC AGAGAACAGT GCCCAAGAGT TCCCCAGCGG CGCCCCGGCC 
AGCTCGGCCA TTGATCTGGA CGCCATGAAC ACCTGCATGT CGCAGGACAT TCCCATGTCC 
ATGCAGACAG TGCACAAGAA GCAGCGCTCC TACGACGTCA TCAGCCCCAT TCAGTTGAAC 
CGCCAACTAG GCGCCTTGCC GCCGGGATGG GAGCAAGCCA AGACCAATGA TGGCCAGATC 
TACTACTTGA ATCATACTAC AAAATCTACG CAGTGGGAGG ATCCCAGAAT CCAATATCGC 
CAGCAGCAGC AAATCTTGAT GGCCGAGCGA ATAAAGCAGA ATGATGTTTT GCAAACTACA 
AAACAAACTA CCACATCGAC CATTGCTAAC AATTTGGGTC CACTGCCGGA TGGTTGGGAG 
CAGGCAGTTA CCGAGTCCGG AGATCTTTAC TTTATAAATC ACATTGATCG AACGACTTCA 
TGGAATGATC CCAGAATGCA ATCTGGGCTT AGCGTGCTCG ACTGCCCAGA TAACTTAGTG 
TCTTCCCTCC AGATTGAGGA TAATCTTTGC AGTAACTTGT TCAATGACGC ACAGGCCATT 
GTAAATCCGC CGTCTTCCCA CAAACCTGAC GATTTGGAAT GGTATAAAAT TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350857.1
CDS	171..1427
Translation	MCACLIAKIILCSFRLYTISAFYMLTTMSASSNTNSLIEKEIDDEDMLSPIKSNNLVVRVNQDTDDNLQALFDSVLNPGDAKRPLQLPLRMRKLPNSFFTPPAPSHSRANSADSTYDAGSQSSINIGNKASIVQQPDGQSPIAAIPQLQIQPSPQHSRLAIHHSRARSSPASLQQNYNVRARSDAAAANNPNANPSSQQQPAGPTFPENSAQEFPSGAPASSAIDLDAMNTCMSQDIPMSMQTVHKKQRSYDVISPIQLNRQLGALPPGWEQAKTNDGQIYYLNHTTKSTQWEDPRIQYRQQQQILMAERIKQNDVLQTTKQTTTSTIANNLGPLPDGWEQAVTESGDLYFINHIDRTTSWNDPRMQSGLSVLDCPDNLVSSLQIEDNLCSNLFNDAQAIVNPPSSHKPDDLEWYKIN

Target ORF information:

RefSeq Version	NM_001363931.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster yorkie (yki), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363931.1

ORF Insert Sequence:

ATGTGCGCGT GCCTAATCGC TAAGATAATT CTATGTAGTT TTCGTTTGTA TACAATAAGT 
GCCTTTTATA TGTTAACGAC GATGTCAGCC AGCAGCAATA CAAACAGCCT GATCGAGAAG 
GAGATCGACG ACGAGGACAT GCTTTCGCCG ATCAAGTCCA ACAACCTGGT GGTGCGGGTC 
AACCAGGACA CGGACGACAA CCTGCAGGCG CTATTCGACA GCGTCCTGAA TCCGGGTGAC 
GCCAAGCGCC CGCTGCAGCT GCCCCTGCGC ATGCGGAAGC TGCCCAACTC CTTCTTCACG 
CCCCCGGCGC CCTCGCACTC GCGGGCCAAC AGCGCCGACT CCACCTACGA CGCGGGCTCC 
CAGTCGAGCA TCAACATCGG GAACAAGGCG TCCATCGTCC AGCAGCCAGA TGGCCAGTCG 
CCCATCGCCG CCATCCCCCA GCTCCAGATT CAGCCGTCTC CCCAGCACAG CCGCCTGGCG 
ATACATCACT CCCGAGCCCG CAGCAGCCCC GCCTCGCTGC AGCAGAACTA CAATGTGCGC 
GCCCGGAGCG ACGCAGCAGC AGCCAACAAT CCGAATGCCA ATCCGAGCAG CCAACAGCAG 
CCCGCTGGGC CCACTTTCCC AGAGAACAGT GCCCAAGAGT TCCCCAGCGG CGCCCCGGCC 
AGCTCGGCCA TTGATCTGGA CGCCATGAAC ACCTGCATGT CGCAGGACAT TCCCATGTCC 
ATGCAGACAG TGCACAAGAA GCAGCGCTCC TACGACGTCA TCAGCCCCAT TCAGTTGAAC 
CGCCAACTAG GCGCCTTGCC GCCGGGATGG GAGCAAGCCA AGACCAATGA TGGCCAGATC 
TACTACTTGA ATCATACTAC AAAATCTACG CAGTGGGAGG ATCCCAGAAT CCAATATCGC 
CAGCAGCAGC AAATCTTGAT GGCCGAGCGA ATAAAGCAGA ATGATGTTTT GCAAACTACA 
AAACAAACTA CCACATCGAC CATTGCTAAC AATTTGGGTC CACTGCCGGA TGGTTGGGAG 
CAGGCAGTTA CCGAGTCCGG AGATCTTTAC TTTATAAATC ACATTGATCG AACGACTTCA 
TGGAATGATC CCAGAATGCA ATCTGGGCTT AGCGTGCTCG ACTGCCCAGA TAACTTAGTG 
TCTTCCCTCC AGATTGAGGA TAATCTTTGC AGTAACTTGT TCAATGACGC ACAGGCCATT 
GTAAATCCGC CGTCTTCCCA CAAACCTGAC GATTTGGAAT GGTATAAAAT TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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yki cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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