eEF5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	eEF5
Entrez Gene ID	37846
Full Name	CG3186 gene product from transcript CG3186-RB
Synonyms	1296,CG3186,Dmel\CG3186,anon-EST:fe2H10,chr2R:19566878..19566998,eIF5A,eIF5a,l(2)01296
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 60B7-60B7

mRNA and Protein(s)

mRNA	Protein	Name
NM_138034.3	NP_611878.1	eukaryotic translation elongation factor 5, isoform A
NM_166654.2	NP_726411.1	eukaryotic translation elongation factor 5, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	EIF5AL1	NP_001093162.1
Saccharomyces cerevisiae (baker's yeast)	ANB1	NP_012581.3
Neurospora crassa	NCU05274	XP_961911.2
Caenorhabditis elegans (roundworm)	iff-2	NP_495807.1
Saccharomyces cerevisiae (baker's yeast)	HYP2	NP_010880.3
Eremothecium gossypii	AGOS_AFR356C	NP_985903.1
Oryza sativa (rice)	Os07g0112800	NP_001058746.1
Drosophila melanogaster (fruit fly)	eIF-5A	NP_611878.1
Kluyveromyces lactis	KLLA0E22199g	XP_454956.1
Schizosaccharomyces pombe (fission yeast)	tif51	NP_594457.1
Arabidopsis thaliana (thale cress)	FBR12	NP_173985.1
Arabidopsis thaliana (thale cress)	ELF5A-1	NP_172848.1
Oryza sativa (rice)	Os12g0507200	NP_001066843.1
Danio rerio (zebrafish)	eif5a	NP_998350.1
Caenorhabditis elegans (roundworm)	iff-1	NP_001255020.1
Schizosaccharomyces pombe (fission yeast)	tif512	NP_596130.2
Oryza sativa (rice)	Os03g0758800	NP_001051336.1
Pan troglodytes (chimpanzee)	LOC450554	XP_507873.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011284	XP_564212.2
Magnaporthe oryzae (rice blast fungus)	MGG_16739	XP_003711866.1
Arabidopsis thaliana (thale cress)	ELF5A-3	NP_177100.1
Oryza sativa (rice)	Os07g0597000	NP_001060174.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results describe the identification and characterization of nero, the Drosophila melanogaster deoxyhypusine hydroxylase (DOHH) homologue, and indicate that nero and eIF5A are required for cell growth and affect autophagy and protein synthesis.
Title: The Drosophila deoxyhypusine hydroxylase homologue nero and its target eIF5A are required for cell growth and the regulation of autophagy.

The following eEF5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eEF5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11112	NM_138034.3 Latest version!	Drosophila melanogaster eukaryotic translation elongation factor 5 (eEF5), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa11112	NM_166654.2 Latest version!	Drosophila melanogaster eukaryotic translation elongation factor 5 (eEF5), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eEF5 ( NM_138034.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611878.1 Drosophila melanogaster eukaryotic translation elongation factor 5 (eEF5), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa11112
Clone ID Related Accession (Same CDS sequence)	NM_138034.3 , NM_166654.2
Accession Version	NM_138034.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation elongation factor 5, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_138034.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGAGT TGGACGATCA ATTCGAGACC ACGGATTCCG GCGCATCGAC GACCTATCCC 
ATGCAATGTT CGGCATTGCG CAAAAACGGA TTCGTCATGC TGAAGTCGCG GCCATGCAAG 
ATTGTCGAGA TGTCTACTTC AAAGACTGGA AAGCACGGAC ACGCCAAGGT TCATATGGTT 
GGCATTGATA TTTTCTCTAA CAAGAAATAT GAAGATATTT GCCCATCTAC TCATAACATG 
GATGTGCCCA ATGTCAAAAG GGAGGACCTT CAGTTGATTG CTATTAGTGA CGATAGCTTC 
CTTACATTGA TGACCGAGAG CGGAGATCTG CGTGAAGACT TGAAGGTTCC AGAGGGCGAA 
TTGGGTGAAC AATTGCGTTT AGATTTTGAT AGTGGCAAGG ACTTACTGTG CACCGTTCTC 
AAGGCGTGCG GAGAGGAGTG CGTTATCGCA ATTAAAACCA ATACTGCTCT GGACAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611878.1
CDS	57..536
Translation	MAELDDQFETTDSGASTTYPMQCSALRKNGFVMLKSRPCKIVEMSTSKTGKHGHAKVHMVGIDIFSNKKYEDICPSTHNMDVPNVKREDLQLIAISDDSFLTLMTESGDLREDLKVPEGELGEQLRLDFDSGKDLLCTVLKACGEECVIAIKTNTALDK

Target ORF information:

RefSeq Version	NM_138034.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation elongation factor 5 (eEF5), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138034.3

ORF Insert Sequence:

ATGGCTGAGT TGGACGATCA ATTCGAGACC ACGGATTCCG GCGCATCGAC GACCTATCCC 
ATGCAATGTT CGGCATTGCG CAAAAACGGA TTCGTCATGC TGAAGTCGCG GCCATGCAAG 
ATTGTCGAGA TGTCTACTTC AAAGACTGGA AAGCACGGAC ACGCCAAGGT TCATATGGTT 
GGCATTGATA TTTTCTCTAA CAAGAAATAT GAAGATATTT GCCCATCTAC TCATAACATG 
GATGTGCCCA ATGTCAAAAG GGAGGACCTT CAGTTGATTG CTATTAGTGA CGATAGCTTC 
CTTACATTGA TGACCGAGAG CGGAGATCTG CGTGAAGACT TGAAGGTTCC AGAGGGCGAA 
TTGGGTGAAC AATTGCGTTT AGATTTTGAT AGTGGCAAGG ACTTACTGTG CACCGTTCTC 
AAGGCGTGCG GAGAGGAGTG CGTTATCGCA ATTAAAACCA ATACTGCTCT GGACAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eEF5 ( NM_166654.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726411.1 Drosophila melanogaster eukaryotic translation elongation factor 5 (eEF5), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa11112
Clone ID Related Accession (Same CDS sequence)	NM_138034.3 , NM_166654.2
Accession Version	NM_166654.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	eukaryotic translation elongation factor 5, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166654.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGAGT TGGACGATCA ATTCGAGACC ACGGATTCCG GCGCATCGAC GACCTATCCC 
ATGCAATGTT CGGCATTGCG CAAAAACGGA TTCGTCATGC TGAAGTCGCG GCCATGCAAG 
ATTGTCGAGA TGTCTACTTC AAAGACTGGA AAGCACGGAC ACGCCAAGGT TCATATGGTT 
GGCATTGATA TTTTCTCTAA CAAGAAATAT GAAGATATTT GCCCATCTAC TCATAACATG 
GATGTGCCCA ATGTCAAAAG GGAGGACCTT CAGTTGATTG CTATTAGTGA CGATAGCTTC 
CTTACATTGA TGACCGAGAG CGGAGATCTG CGTGAAGACT TGAAGGTTCC AGAGGGCGAA 
TTGGGTGAAC AATTGCGTTT AGATTTTGAT AGTGGCAAGG ACTTACTGTG CACCGTTCTC 
AAGGCGTGCG GAGAGGAGTG CGTTATCGCA ATTAAAACCA ATACTGCTCT GGACAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726411.1
CDS	108..587
Translation	MAELDDQFETTDSGASTTYPMQCSALRKNGFVMLKSRPCKIVEMSTSKTGKHGHAKVHMVGIDIFSNKKYEDICPSTHNMDVPNVKREDLQLIAISDDSFLTLMTESGDLREDLKVPEGELGEQLRLDFDSGKDLLCTVLKACGEECVIAIKTNTALDK

Target ORF information:

RefSeq Version	NM_166654.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster eukaryotic translation elongation factor 5 (eEF5), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166654.2

ORF Insert Sequence:

ATGGCTGAGT TGGACGATCA ATTCGAGACC ACGGATTCCG GCGCATCGAC GACCTATCCC 
ATGCAATGTT CGGCATTGCG CAAAAACGGA TTCGTCATGC TGAAGTCGCG GCCATGCAAG 
ATTGTCGAGA TGTCTACTTC AAAGACTGGA AAGCACGGAC ACGCCAAGGT TCATATGGTT 
GGCATTGATA TTTTCTCTAA CAAGAAATAT GAAGATATTT GCCCATCTAC TCATAACATG 
GATGTGCCCA ATGTCAAAAG GGAGGACCTT CAGTTGATTG CTATTAGTGA CGATAGCTTC 
CTTACATTGA TGACCGAGAG CGGAGATCTG CGTGAAGACT TGAAGGTTCC AGAGGGCGAA 
TTGGGTGAAC AATTGCGTTT AGATTTTGAT AGTGGCAAGG ACTTACTGTG CACCGTTCTC 
AAGGCGTGCG GAGAGGAGTG CGTTATCGCA ATTAAAACCA ATACTGCTCT GGACAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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eEF5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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