Chi cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chi
Entrez Gene ID	37837
Full Name	Chip
Synonyms	CG3924,CHIP,Dmel\CG3924,LDB,Ldb,chip,dLDB/Chip,dLdb,l(2)04405,l(2)k04405
General protein information	Preferred Names Chip Names CG3924 gene product from transcript CG3924-RA CG3924-PA CG3924-PB Chi-PA Chi-PB chip
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 60B5-60B5\|2-106.8 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_057734.4	NP_477082.1	chip, isoform A
NM_166650.2	NP_726403.2	chip, isoform B

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	ldb2	XP_002938682.1
Homo sapiens (human)	LDB2	NP_001281.1
	LDB2	XP_001101329.1
Mus musculus (house mouse)	Ldb2	NP_034828.3
Rattus norvegicus (Norway rat)	Ldb2	NP_001099479.1
Gallus gallus (chicken)	LDB2	NP_990160.1
Caenorhabditis elegans (roundworm)	ldb-1	NP_509844.3
Danio rerio (zebrafish)	ldb2a	NP_571389.1
Pan troglodytes (chimpanzee)	LDB2	XP_001161726.1
Canis lupus familiaris (dog)	LDB2	XP_005618602.1
Drosophila melanogaster (fruit fly)	Chi	NP_726403.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004691	XP_318175.4
Bos taurus (cattle)	LDB2	NP_001040076.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Drosophila CHIP protects against mitochondrial dysfunction by acting downstream of Pink1 in parallel with Parkin

Chip is required for normal heart development and interacts with tup in this context.
Title: Chip/Ldb1 interacts with Tailup/islet1 to regulate cardiac gene expression in Drosophila.

The function of Chi and dLMO on Notch signaling is dependent on the regulation of fng transcription.
Title: Chi and dLMO function antagonistically on Notch signaling through directly regulation of fng transcription.

The authors use a proteomics approach to discover the Chip/LDB-SSDP (ChiLS) complex as the ligand specifically binding to NPF motif of Pygo proteins.
Title: An ancient Pygo-dependent Wnt enhanceosome integrated by Chip/LDB-SSDP.

Chip is required for posteclosion behavior in Drosophila.
Title: Chip is required for posteclosion behavior in Drosophila.

The following Chi gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chi cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11100	NM_057734.4 Latest version!	Drosophila melanogaster chip, transcript variant A (Chi), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa11101	NM_166650.2 Latest version!	Drosophila melanogaster chip, transcript variant B (Chi), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chi ( NM_057734.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477082.1 Drosophila melanogaster chip, transcript variant A (Chi), mRNA.

Price & Availability↑

CloneID	OFa11100
Clone ID Related Accession (Same CDS sequence)	NM_057734.4
Accession Version	NM_057734.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chip, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_057734.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATCGTA GGGGTTTGAA CGCTGGCAAT ACGATGACTT CTCAAGCGAA CATTGACGAC 
GGCAGCTGGA AGGCGGTCTC CGAGGGCGGA TCAATGCTGC CCGCGTCCAA TTCGGCGGTC 
CTCAATCCGG ACGGGAGCAA CCAGAGCGGC TTCGCGCAGG GCGGCCTGCC GTACAACTCG 
GCCGGGAATC CGTACCCGCC CGCCGGCCAA TCCTCGCCAG CCGGCAACCA GTCCATCGTG 
TTCCAGAACT CCAACCAGCC TGGCTCGAAC ACACCTCAGT ATACCTCCTC ACCTGCTCCC 
TCGGGCTCAT CGACACCCGG ACCTGTTGGT GCGCAGAACA TTCCCGGCAA CTACCCGCAG 
TCGGCGACGG CGGGCAATTT CAATGGTCCA GTTGGCGGGC CCTTCGGCTC GCCATCCTCG 
GGACTCGGCC AGTTCAGCCG GCCGGCCAGC TCGGGCACTC CGTTTAACAG TGGCCAGGCC 
GGGCACTTCT CATCGCCCAC GGTATTCAGC GTTGGCGGGC AATTCAATCC GATGCCGCCG 
GCGTCGCCAT TCGGCCACGG AGGCAACCAC CCGATGATGG GCGGGCCGCA GCAGATGGAA 
CGCATCGACC AGGGCTTCAG GCGGCACAAT TCCTACTTTA GCCACACGGA ACACCGCGTC 
TTTGAGCTAA ACAAGCGGCT GCAGCAGCGC AACGAGGAGA GCGACAACTG CTGGTGGGAC 
TCGTTTACCA CGGAGTTCTT CGAGGATGAT GCCCGGCTGA CCATTCTGTT CTGCCTGGAG 
GACGGACCGA AGCGGTACAC CATCGGGCGC ACGCTCATCC CGCGCTTCTT CCGCAGCATA 
TACGAGGGCG GCGTTTCGGA CCTGTACTTT CAGCTGAAGC ATGCCAAGGA GTCGTTCCAC 
AACACGTCCA TCACGTTGGA CTGCGACCAG TGCACGGTGA TCACGCAGCA CGGCAAGCCC 
TTCTTCACGA AGGTCTGCGC CGACGCAAGA CTGATCTTGG AGTTCATGTA CGACGACTAC 
ATGCGCATCA AGTCATGGCA CATGACCATC AAGGGACACC GCGAGCTCAT TCCAAGGTCC 
GTGATCGGCA CCAGTCTGCC GCCTGACCCG ATGCTGCTAG ATCAAATAAC CAAGAACATC 
ACGCGCGCTG GCATCACCAA CTCCACCCTC AACTATCTGC GCCTCTGCGT CATCCTCGAA 
CCGATGCAGG AGCTGATGTC GCGGCACAAG GCGTACGCAC TGAGTCCGCG CGACTGCCTG 
AAGACAACGT TGTTCCAGAA GTGGCAGCGC ATGGTGGCTC CGCCCGGCAA GAAGGATCCG 
CAACGACCAC CCAACAAGAG GCGCAAACGG AAGGGCTCCA ACTCGGGTGG TGGCAACAAC 
TCGAACACTC CGCCTGTGAC GAACCAGAAG CGTTCCCCCT CCGGTCCCAG TTTCTCCCTC 
TCCTCCCAGG ACGTGATGGT GGTGGGCGAG CCGACGCTGA TGGGCGGCGA GTTTGGTGAG 
GAGGACGAGC GGCTGATCAC CCGGCTAGAG AACACGCAAT ACGACGGAAC CAATGCCGTG 
GAGCACGATA ATCACACCGG CTTTGGACAC GCCGACTCGC CCATATCCGG CTCGAATCCG 
TGGAGCATCG ACCGGGCGGG AGCCATCCCG GCCAGCCCTG GGAACGGAGC TGCCCCGCAG 
AACAACGCGA ATATATCTGA CATAGATAAA AAGAGCCCCA TTGTATCGCA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477082.1
CDS	157..1890
Translation	MNRRGLNAGNTMTSQANIDDGSWKAVSEGGSMLPASNSAVLNPDGSNQSGFAQGGLPYNSAGNPYPPAGQSSPAGNQSIVFQNSNQPGSNTPQYTSSPAPSGSSTPGPVGAQNIPGNYPQSATAGNFNGPVGGPFGSPSSGLGQFSRPASSGTPFNSGQAGHFSSPTVFSVGGQFNPMPPASPFGHGGNHPMMGGPQQMERIDQGFRRHNSYFSHTEHRVFELNKRLQQRNEESDNCWWDSFTTEFFEDDARLTILFCLEDGPKRYTIGRTLIPRFFRSIYEGGVSDLYFQLKHAKESFHNTSITLDCDQCTVITQHGKPFFTKVCADARLILEFMYDDYMRIKSWHMTIKGHRELIPRSVIGTSLPPDPMLLDQITKNITRAGITNSTLNYLRLCVILEPMQELMSRHKAYALSPRDCLKTTLFQKWQRMVAPPGKKDPQRPPNKRRKRKGSNSGGGNNSNTPPVTNQKRSPSGPSFSLSSQDVMVVGEPTLMGGEFGEEDERLITRLENTQYDGTNAVEHDNHTGFGHADSPISGSNPWSIDRAGAIPASPGNGAAPQNNANISDIDKKSPIVSQ

Target ORF information:

RefSeq Version	NM_057734.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chip, transcript variant A (Chi), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057734.4

ORF Insert Sequence:

ATGAATCGTA GGGGTTTGAA CGCTGGCAAT ACGATGACTT CTCAAGCGAA CATTGACGAC 
GGCAGCTGGA AGGCGGTCTC CGAGGGCGGA TCAATGCTGC CCGCGTCCAA TTCGGCGGTC 
CTCAATCCGG ACGGGAGCAA CCAGAGCGGC TTCGCGCAGG GCGGCCTGCC GTACAACTCG 
GCCGGGAATC CGTACCCGCC CGCCGGCCAA TCCTCGCCAG CCGGCAACCA GTCCATCGTG 
TTCCAGAACT CCAACCAGCC TGGCTCGAAC ACACCTCAGT ATACCTCCTC ACCTGCTCCC 
TCGGGCTCAT CGACACCCGG ACCTGTTGGT GCGCAGAACA TTCCCGGCAA CTACCCGCAG 
TCGGCGACGG CGGGCAATTT CAATGGTCCA GTTGGCGGGC CCTTCGGCTC GCCATCCTCG 
GGACTCGGCC AGTTCAGCCG GCCGGCCAGC TCGGGCACTC CGTTTAACAG TGGCCAGGCC 
GGGCACTTCT CATCGCCCAC GGTATTCAGC GTTGGCGGGC AATTCAATCC GATGCCGCCG 
GCGTCGCCAT TCGGCCACGG AGGCAACCAC CCGATGATGG GCGGGCCGCA GCAGATGGAA 
CGCATCGACC AGGGCTTCAG GCGGCACAAT TCCTACTTTA GCCACACGGA ACACCGCGTC 
TTTGAGCTAA ACAAGCGGCT GCAGCAGCGC AACGAGGAGA GCGACAACTG CTGGTGGGAC 
TCGTTTACCA CGGAGTTCTT CGAGGATGAT GCCCGGCTGA CCATTCTGTT CTGCCTGGAG 
GACGGACCGA AGCGGTACAC CATCGGGCGC ACGCTCATCC CGCGCTTCTT CCGCAGCATA 
TACGAGGGCG GCGTTTCGGA CCTGTACTTT CAGCTGAAGC ATGCCAAGGA GTCGTTCCAC 
AACACGTCCA TCACGTTGGA CTGCGACCAG TGCACGGTGA TCACGCAGCA CGGCAAGCCC 
TTCTTCACGA AGGTCTGCGC CGACGCAAGA CTGATCTTGG AGTTCATGTA CGACGACTAC 
ATGCGCATCA AGTCATGGCA CATGACCATC AAGGGACACC GCGAGCTCAT TCCAAGGTCC 
GTGATCGGCA CCAGTCTGCC GCCTGACCCG ATGCTGCTAG ATCAAATAAC CAAGAACATC 
ACGCGCGCTG GCATCACCAA CTCCACCCTC AACTATCTGC GCCTCTGCGT CATCCTCGAA 
CCGATGCAGG AGCTGATGTC GCGGCACAAG GCGTACGCAC TGAGTCCGCG CGACTGCCTG 
AAGACAACGT TGTTCCAGAA GTGGCAGCGC ATGGTGGCTC CGCCCGGCAA GAAGGATCCG 
CAACGACCAC CCAACAAGAG GCGCAAACGG AAGGGCTCCA ACTCGGGTGG TGGCAACAAC 
TCGAACACTC CGCCTGTGAC GAACCAGAAG CGTTCCCCCT CCGGTCCCAG TTTCTCCCTC 
TCCTCCCAGG ACGTGATGGT GGTGGGCGAG CCGACGCTGA TGGGCGGCGA GTTTGGTGAG 
GAGGACGAGC GGCTGATCAC CCGGCTAGAG AACACGCAAT ACGACGGAAC CAATGCCGTG 
GAGCACGATA ATCACACCGG CTTTGGACAC GCCGACTCGC CCATATCCGG CTCGAATCCG 
TGGAGCATCG ACCGGGCGGG AGCCATCCCG GCCAGCCCTG GGAACGGAGC TGCCCCGCAG 
AACAACGCGA ATATATCTGA CATAGATAAA AAGAGCCCCA TTGTATCGCA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chi ( NM_166650.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726403.2 Drosophila melanogaster chip, transcript variant B (Chi), mRNA.

Price & Availability↑

CloneID	OFa11101
Clone ID Related Accession (Same CDS sequence)	NM_166650.2
Accession Version	NM_166650.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chip, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_166650.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGTGCG ACATACAGAC AAGCCGACGC GAATGCCCGA TAGAACCGAC CACAGGCATG 
AATCGTAGGG GTTTGAACGC TGGCAATACG ATGACTTCTC AAGCGAACAT TGACGACGGC 
AGCTGGAAGG CGGTCTCCGA GGGCGGATCA ATGCTGCCCG CGTCCAATTC GGCGGTCCTC 
AATCCGGACG GGAGCAACCA GAGCGGCTTC GCGCAGGGCG GCCTGCCGTA CAACTCGGCC 
GGGAATCCGT ACCCGCCCGC CGGCCAATCC TCGCCAGCCG GCAACCAGTC CATCGTGTTC 
CAGAACTCCA ACCAGCCTGG CTCGAACACA CCTCAGTATA CCTCCTCACC TGCTCCCTCG 
GGCTCATCGA CACCCGGACC TGTTGGTGCG CAGAACATTC CCGGCAACTA CCCGCAGTCG 
GCGACGGCGG GCAATTTCAA TGGTCCAGTT GGCGGGCCCT TCGGCTCGCC ATCCTCGGGA 
CTCGGCCAGT TCAGCCGGCC GGCCAGCTCG GGCACTCCGT TTAACAGTGG CCAGGCCGGG 
CACTTCTCAT CGCCCACGGT ATTCAGCGTT GGCGGGCAAT TCAATCCGAT GCCGCCGGCG 
TCGCCATTCG GCCACGGAGG CAACCACCCG ATGATGGGCG GGCCGCAGCA GATGGAACGC 
ATCGACCAGG GCTTCAGGCG GCACAATTCC TACTTTAGCC ACACGGAACA CCGCGTCTTT 
GAGCTAAACA AGCGGCTGCA GCAGCGCAAC GAGGAGAGCG ACAACTGCTG GTGGGACTCG 
TTTACCACGG AGTTCTTCGA GGATGATGCC CGGCTGACCA TTCTGTTCTG CCTGGAGGAC 
GGACCGAAGC GGTACACCAT CGGGCGCACG CTCATCCCGC GCTTCTTCCG CAGCATATAC 
GAGGGCGGCG TTTCGGACCT GTACTTTCAG CTGAAGCATG CCAAGGAGTC GTTCCACAAC 
ACGTCCATCA CGTTGGACTG CGACCAGTGC ACGGTGATCA CGCAGCACGG CAAGCCCTTC 
TTCACGAAGG TCTGCGCCGA CGCAAGACTG ATCTTGGAGT TCATGTACGA CGACTACATG 
CGCATCAAGT CATGGCACAT GACCATCAAG GGACACCGCG AGCTCATTCC AAGGTCCGTG 
ATCGGCACCA GTCTGCCGCC TGACCCGATG CTGCTAGATC AAATAACCAA GAACATCACG 
CGCGCTGGCA TCACCAACTC CACCCTCAAC TATCTGCGCC TCTGCGTCAT CCTCGAACCG 
ATGCAGGAGC TGATGTCGCG GCACAAGGCG TACGCACTGA GTCCGCGCGA CTGCCTGAAG 
ACAACGTTGT TCCAGAAGTG GCAGCGCATG GTGGCTCCGC CCGGCAAGAA GGATCCGCAA 
CGACCACCCA ACAAGAGGCG CAAACGGAAG GGCTCCAACT CGGGTGGTGG CAACAACTCG 
AACACTCCGC CTGTGACGAA CCAGAAGCGT TCCCCCTCCG GTCCCAGTTT CTCCCTCTCC 
TCCCAGGACG TGATGGTGGT GGGCGAGCCG ACGCTGATGG GCGGCGAGTT TGGTGAGGAG 
GACGAGCGGC TGATCACCCG GCTAGAGAAC ACGCAATACG ACGGAACCAA TGCCGTGGAG 
CACGATAATC ACACCGGCTT TGGACACGCC GACTCGCCCA TATCCGGCTC GAATCCGTGG 
AGCATCGACC GGGCGGGAGC CATCCCGGCC AGCCCTGGGA ACGGAGCTGC CCCGCAGAAC 
AACGCGAATA TATCTGACAT AGATAAAAAG AGCCCCATTG TATCGCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726403.2
CDS	69..1859
Translation	MQCDIQTSRRECPIEPTTGMNRRGLNAGNTMTSQANIDDGSWKAVSEGGSMLPASNSAVLNPDGSNQSGFAQGGLPYNSAGNPYPPAGQSSPAGNQSIVFQNSNQPGSNTPQYTSSPAPSGSSTPGPVGAQNIPGNYPQSATAGNFNGPVGGPFGSPSSGLGQFSRPASSGTPFNSGQAGHFSSPTVFSVGGQFNPMPPASPFGHGGNHPMMGGPQQMERIDQGFRRHNSYFSHTEHRVFELNKRLQQRNEESDNCWWDSFTTEFFEDDARLTILFCLEDGPKRYTIGRTLIPRFFRSIYEGGVSDLYFQLKHAKESFHNTSITLDCDQCTVITQHGKPFFTKVCADARLILEFMYDDYMRIKSWHMTIKGHRELIPRSVIGTSLPPDPMLLDQITKNITRAGITNSTLNYLRLCVILEPMQELMSRHKAYALSPRDCLKTTLFQKWQRMVAPPGKKDPQRPPNKRRKRKGSNSGGGNNSNTPPVTNQKRSPSGPSFSLSSQDVMVVGEPTLMGGEFGEEDERLITRLENTQYDGTNAVEHDNHTGFGHADSPISGSNPWSIDRAGAIPASPGNGAAPQNNANISDIDKKSPIVSQ

Target ORF information:

RefSeq Version	NM_166650.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chip, transcript variant B (Chi), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166650.2

ORF Insert Sequence:

ATGCAGTGCG ACATACAGAC AAGCCGACGC GAATGCCCGA TAGAACCGAC CACAGGCATG 
AATCGTAGGG GTTTGAACGC TGGCAATACG ATGACTTCTC AAGCGAACAT TGACGACGGC 
AGCTGGAAGG CGGTCTCCGA GGGCGGATCA ATGCTGCCCG CGTCCAATTC GGCGGTCCTC 
AATCCGGACG GGAGCAACCA GAGCGGCTTC GCGCAGGGCG GCCTGCCGTA CAACTCGGCC 
GGGAATCCGT ACCCGCCCGC CGGCCAATCC TCGCCAGCCG GCAACCAGTC CATCGTGTTC 
CAGAACTCCA ACCAGCCTGG CTCGAACACA CCTCAGTATA CCTCCTCACC TGCTCCCTCG 
GGCTCATCGA CACCCGGACC TGTTGGTGCG CAGAACATTC CCGGCAACTA CCCGCAGTCG 
GCGACGGCGG GCAATTTCAA TGGTCCAGTT GGCGGGCCCT TCGGCTCGCC ATCCTCGGGA 
CTCGGCCAGT TCAGCCGGCC GGCCAGCTCG GGCACTCCGT TTAACAGTGG CCAGGCCGGG 
CACTTCTCAT CGCCCACGGT ATTCAGCGTT GGCGGGCAAT TCAATCCGAT GCCGCCGGCG 
TCGCCATTCG GCCACGGAGG CAACCACCCG ATGATGGGCG GGCCGCAGCA GATGGAACGC 
ATCGACCAGG GCTTCAGGCG GCACAATTCC TACTTTAGCC ACACGGAACA CCGCGTCTTT 
GAGCTAAACA AGCGGCTGCA GCAGCGCAAC GAGGAGAGCG ACAACTGCTG GTGGGACTCG 
TTTACCACGG AGTTCTTCGA GGATGATGCC CGGCTGACCA TTCTGTTCTG CCTGGAGGAC 
GGACCGAAGC GGTACACCAT CGGGCGCACG CTCATCCCGC GCTTCTTCCG CAGCATATAC 
GAGGGCGGCG TTTCGGACCT GTACTTTCAG CTGAAGCATG CCAAGGAGTC GTTCCACAAC 
ACGTCCATCA CGTTGGACTG CGACCAGTGC ACGGTGATCA CGCAGCACGG CAAGCCCTTC 
TTCACGAAGG TCTGCGCCGA CGCAAGACTG ATCTTGGAGT TCATGTACGA CGACTACATG 
CGCATCAAGT CATGGCACAT GACCATCAAG GGACACCGCG AGCTCATTCC AAGGTCCGTG 
ATCGGCACCA GTCTGCCGCC TGACCCGATG CTGCTAGATC AAATAACCAA GAACATCACG 
CGCGCTGGCA TCACCAACTC CACCCTCAAC TATCTGCGCC TCTGCGTCAT CCTCGAACCG 
ATGCAGGAGC TGATGTCGCG GCACAAGGCG TACGCACTGA GTCCGCGCGA CTGCCTGAAG 
ACAACGTTGT TCCAGAAGTG GCAGCGCATG GTGGCTCCGC CCGGCAAGAA GGATCCGCAA 
CGACCACCCA ACAAGAGGCG CAAACGGAAG GGCTCCAACT CGGGTGGTGG CAACAACTCG 
AACACTCCGC CTGTGACGAA CCAGAAGCGT TCCCCCTCCG GTCCCAGTTT CTCCCTCTCC 
TCCCAGGACG TGATGGTGGT GGGCGAGCCG ACGCTGATGG GCGGCGAGTT TGGTGAGGAG 
GACGAGCGGC TGATCACCCG GCTAGAGAAC ACGCAATACG ACGGAACCAA TGCCGTGGAG 
CACGATAATC ACACCGGCTT TGGACACGCC GACTCGCCCA TATCCGGCTC GAATCCGTGG 
AGCATCGACC GGGCGGGAGC CATCCCGGCC AGCCCTGGGA ACGGAGCTGC CCCGCAGAAC 
AACGCGAATA TATCTGACAT AGATAAAAAG AGCCCCATTG TATCGCAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Chi cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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