thoc5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	thoc5
Entrez Gene ID	37810
Full Name	CG2980 gene product from transcript CG2980-RA
Synonyms	CG2980,Dmel\CG2980,THOC5,Thoc5,garm
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 60A13-60A13

mRNA and Protein(s)

mRNA	Protein	Name
NM_138012.3	NP_611856.1	thoc5

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	THOC5	XP_001135376.2
Canis lupus familiaris (dog)	THOC5	XP_534728.2
Danio rerio (zebrafish)	thoc5	NP_997857.1
Homo sapiens (human)	THOC5	NP_001002877.1
Bos taurus (cattle)	THOC5	NP_001077185.1
Mus musculus (house mouse)	Thoc5	NP_766026.1
Rattus norvegicus (Norway rat)	Thoc5	NP_001012153.1
Gallus gallus (chicken)	THOC5	NP_001006195.1
Drosophila melanogaster (fruit fly)	thoc5	NP_611856.1
	LOC714842	XP_002808014.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004746	XP_001689094.1
Xenopus tropicalis (tropical clawed frog)	thoc5	NP_001016827.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Flies lacking THOC5 showed a meiotic arrest phenotype with severe nucleolar disruption in primary spermatocytes.
Title: The THO complex is required for nucleolar integrity in Drosophila spermatocytes.

The following thoc5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the thoc5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13223	NM_138012.3 Latest version!	Drosophila melanogaster thoc5 (thoc5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

thoc5 ( NM_138012.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611856.1 Drosophila melanogaster thoc5 (thoc5), mRNA.

Price & Availability↑

CloneID	OFa13223
Clone ID Related Accession (Same CDS sequence)	NM_138012.3
Accession Version	NM_138012.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	thoc5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_138012.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAACAA TATTGAACAC GCAGTTCGAT CAGCTGCAAA AGGCCATAGA GGGAATTAAG 
GAGGCGAAGA GCAAGGGGCA ATCGACGGAT GAGGCTCGCA TTGCCACCAC CTTGATGTTC 
GTGGCTCTGA AGCACGCCAA CCGCGAGGTG AAGCACAAGA TCAAAGCGGG GCGCGACAAG 
CTGCACCTGG AAAAGTGCAA GGTGGACCTC AGCCGGCTGC AGCTGCAGAA TCTTCTGTAC 
GAAGTGAGCC ACCTGAAGAA GGAGATTGTG CGCTGCCAGA AGTTCAAGAG CAGGGACACC 
AGCTTGGAGC TGCTCTCGGA AGCCGAGTAC TCCAGCGAGC TGCCAGACAT GCCCAACGAC 
GCGGAGGAGC TGTCCAGCCA CAAGCAGCAC CTTCATCGCC TCGACTGCGA GCTGCGTCTG 
CGGAAGGCAC TCGACAAGCA GTACAGCGCC CTGCTGAGCT CCAAGCAGGA ACTGCTCCAG 
GACAATCTTA GCCACGCCCA GCGGTACGTC TCCTTCGCAC CCGCCTTGCG CACTCTGCAG 
AGCGCCACAA AGCCGCTGCA CGATGCACTC CAGCTATCCC TGGACGTGGA ATGGAAGCTA 
AGCGCAGTGG TCAAGTACCT GCCCAAGCCG CTCTACGTTT TGTTCATTGT CCTGCAATCG 
CTGCAGCGGG TCAGCGAGGA GCAGTCCTTC ACCGCCGAGG TGGTTGGCTA TGAGAGCGAG 
GCTCAAATGC AGGAGTTGCT TAAGGAGAAA AAGTGCTCCA CATTGAACTA CAGACAGACT 
AAAAAGGAAA GTGGAGACCG GGAAAAGATT GCCGATAAAA ACAACTTGGA CGACTCACTC 
GCTCCTCACC CACTACACAT TTGTTTGACG ATTGGAGCGG CTGGATCGAA TCAGCTCGTT 
TTGCAGATTC GCTACTTTGA TTTTATCAAG TGTGCCACTG TCTGGGGCCA ACTTAACTGC 
TCCAGGAATG CCAACGGCCA GGGTGACACA AACTTTGTTC ACCACCTATT GCGGCATTTG 
TACCCCAATG ACTTGGGCAA TGAATTACCC ATTCCGGGAA TCCAATACGA GCTACGAAGC 
TCGGATTTAA CAGCCGAGGA ATGTGTGCGC TACCTCAAGG CCAAGGACTA CGGAAAACCC 
TTCTGTTGGC TGCAAAGTAT GTGCTCGATT GCAACCGTCA ACAGTTCGAT GCTGTACAAA 
CACGAACTGA ACTTGAATAA GAACAACAAG GAGATTATTG ACCGCATTGC CAGGCGCTTG 
AACTCTTGGC AGCGTTTAAG CCAGCAGATT CGCGGGCTGA CCTTCAAGGA TATCGATTTG 
TATAGCTTGC AGGAGAACAT CTATCCAGCC GGCCTGTCGT GCAGTCTCGT CCAATGGACT 
GCCATCACAC ATGAGGAGCT TCACTCGCAA ATTTCAGACG CACTGAATTC TTCGTCAGCG 
GACAAGGGTA TTACGTACAA CTCTTACCGC GCTGTCATCG TCCGGGGATC GGCCAAAATG 
GAGTGCTTTA TTAGAATACC GAGTAACTAT CCACTGGAAA TGCCTCTATG GATTCTGAAT 
GTGCACTGGA ACGGATGCCT TACCGCGCAA AACAATTCGG CGATCAAGAT GATGGAGTTC 
TGGACCAACT CCCTGCAGCC CAAACATTTG GAGCCCAACG ACTGCATCCT GTACGCGCAG 
CTCTTCCGCA CCATCTACAG CTTCGATATA TTCCTCGAAA CCGAGGGATC CATGCAAAAC 
ACCATTGAGT ACAACAAGGA GAAGCCTTAC ATAAGCGCCT TCGCCAAGCG GAGCCGCTCA 
CGCCCCTACA AATACATCAA GAAGGGATCG GTCTACGCGT TCAAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611856.1
CDS	74..1924
Translation	METILNTQFDQLQKAIEGIKEAKSKGQSTDEARIATTLMFVALKHANREVKHKIKAGRDKLHLEKCKVDLSRLQLQNLLYEVSHLKKEIVRCQKFKSRDTSLELLSEAEYSSELPDMPNDAEELSSHKQHLHRLDCELRLRKALDKQYSALLSSKQELLQDNLSHAQRYVSFAPALRTLQSATKPLHDALQLSLDVEWKLSAVVKYLPKPLYVLFIVLQSLQRVSEEQSFTAEVVGYESEAQMQELLKEKKCSTLNYRQTKKESGDREKIADKNNLDDSLAPHPLHICLTIGAAGSNQLVLQIRYFDFIKCATVWGQLNCSRNANGQGDTNFVHHLLRHLYPNDLGNELPIPGIQYELRSSDLTAEECVRYLKAKDYGKPFCWLQSMCSIATVNSSMLYKHELNLNKNNKEIIDRIARRLNSWQRLSQQIRGLTFKDIDLYSLQENIYPAGLSCSLVQWTAITHEELHSQISDALNSSSADKGITYNSYRAVIVRGSAKMECFIRIPSNYPLEMPLWILNVHWNGCLTAQNNSAIKMMEFWTNSLQPKHLEPNDCILYAQLFRTIYSFDIFLETEGSMQNTIEYNKEKPYISAFAKRSRSRPYKYIKKGSVYAFKQ

Target ORF information:

RefSeq Version	NM_138012.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster thoc5 (thoc5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138012.3

ORF Insert Sequence:

ATGGAAACAA TATTGAACAC GCAGTTCGAT CAGCTGCAAA AGGCCATAGA GGGAATTAAG 
GAGGCGAAGA GCAAGGGGCA ATCGACGGAT GAGGCTCGCA TTGCCACCAC CTTGATGTTC 
GTGGCTCTGA AGCACGCCAA CCGCGAGGTG AAGCACAAGA TCAAAGCGGG GCGCGACAAG 
CTGCACCTGG AAAAGTGCAA GGTGGACCTC AGCCGGCTGC AGCTGCAGAA TCTTCTGTAC 
GAAGTGAGCC ACCTGAAGAA GGAGATTGTG CGCTGCCAGA AGTTCAAGAG CAGGGACACC 
AGCTTGGAGC TGCTCTCGGA AGCCGAGTAC TCCAGCGAGC TGCCAGACAT GCCCAACGAC 
GCGGAGGAGC TGTCCAGCCA CAAGCAGCAC CTTCATCGCC TCGACTGCGA GCTGCGTCTG 
CGGAAGGCAC TCGACAAGCA GTACAGCGCC CTGCTGAGCT CCAAGCAGGA ACTGCTCCAG 
GACAATCTTA GCCACGCCCA GCGGTACGTC TCCTTCGCAC CCGCCTTGCG CACTCTGCAG 
AGCGCCACAA AGCCGCTGCA CGATGCACTC CAGCTATCCC TGGACGTGGA ATGGAAGCTA 
AGCGCAGTGG TCAAGTACCT GCCCAAGCCG CTCTACGTTT TGTTCATTGT CCTGCAATCG 
CTGCAGCGGG TCAGCGAGGA GCAGTCCTTC ACCGCCGAGG TGGTTGGCTA TGAGAGCGAG 
GCTCAAATGC AGGAGTTGCT TAAGGAGAAA AAGTGCTCCA CATTGAACTA CAGACAGACT 
AAAAAGGAAA GTGGAGACCG GGAAAAGATT GCCGATAAAA ACAACTTGGA CGACTCACTC 
GCTCCTCACC CACTACACAT TTGTTTGACG ATTGGAGCGG CTGGATCGAA TCAGCTCGTT 
TTGCAGATTC GCTACTTTGA TTTTATCAAG TGTGCCACTG TCTGGGGCCA ACTTAACTGC 
TCCAGGAATG CCAACGGCCA GGGTGACACA AACTTTGTTC ACCACCTATT GCGGCATTTG 
TACCCCAATG ACTTGGGCAA TGAATTACCC ATTCCGGGAA TCCAATACGA GCTACGAAGC 
TCGGATTTAA CAGCCGAGGA ATGTGTGCGC TACCTCAAGG CCAAGGACTA CGGAAAACCC 
TTCTGTTGGC TGCAAAGTAT GTGCTCGATT GCAACCGTCA ACAGTTCGAT GCTGTACAAA 
CACGAACTGA ACTTGAATAA GAACAACAAG GAGATTATTG ACCGCATTGC CAGGCGCTTG 
AACTCTTGGC AGCGTTTAAG CCAGCAGATT CGCGGGCTGA CCTTCAAGGA TATCGATTTG 
TATAGCTTGC AGGAGAACAT CTATCCAGCC GGCCTGTCGT GCAGTCTCGT CCAATGGACT 
GCCATCACAC ATGAGGAGCT TCACTCGCAA ATTTCAGACG CACTGAATTC TTCGTCAGCG 
GACAAGGGTA TTACGTACAA CTCTTACCGC GCTGTCATCG TCCGGGGATC GGCCAAAATG 
GAGTGCTTTA TTAGAATACC GAGTAACTAT CCACTGGAAA TGCCTCTATG GATTCTGAAT 
GTGCACTGGA ACGGATGCCT TACCGCGCAA AACAATTCGG CGATCAAGAT GATGGAGTTC 
TGGACCAACT CCCTGCAGCC CAAACATTTG GAGCCCAACG ACTGCATCCT GTACGCGCAG 
CTCTTCCGCA CCATCTACAG CTTCGATATA TTCCTCGAAA CCGAGGGATC CATGCAAAAC 
ACCATTGAGT ACAACAAGGA GAAGCCTTAC ATAAGCGCCT TCGCCAAGCG GAGCCGCTCA 
CGCCCCTACA AATACATCAA GAAGGGATCG GTCTACGCGT TCAAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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thoc5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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