Galphas cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Galphas
Entrez Gene ID	37805
Full Name	G protein alpha s subunit
Synonyms	CG2835,DGs,DGsalpha,Dmel\CG2835,G-5alpha,G-alpha-60A,G-salpha 60A,G-salpha60A,GNAS/L,GSalpha,G[[S]]alpha,G[[alphas]],G[[s]],G[[s]]-alpha-60A,G[[s]]alpha,Gaalpha,Galpha60A,GalphaS,Galpha[[s]],Galpha[[s]]60A,Galphas60A,Gs,Gs-alpha,Gs-alpha60A,Gsalpha,Gsalpha60A,dG[[alphas]],dgs,dgsalpha,galphas
General protein information	Preferred Names G protein alpha s subunit Names CG2835 gene product from transcript CG2835-RB CG2835-PA CG2835-PB CG2835-PC CG2835-PD CG2835-PE G protein salpha 60A G-alpha s Galphas-PA Galphas-PB Galphas-PC Galphas-PD Galphas-PE stimulatory alpha subunit of G protein
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 60A12-60A13

mRNA and Protein(s)

mRNA	Protein	Name
NM_058157.5	NP_477505.1	G protein alpha s subunit, isoform B
NM_058158.5	NP_477506.1	G protein alpha s subunit, isoform A
NM_166640.3	NP_726388.1	G protein alpha s subunit, isoform C
NM_001169812.2	NP_001163283.1	G protein alpha s subunit, isoform D
NM_001299869.1	NP_001286798.1	G protein alpha s subunit, isoform E

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GNAS	NP_001003263.1
Rattus norvegicus (Norway rat)	Gnas	NP_068617.6
Caenorhabditis elegans (roundworm)	gsa-1	NP_490817.3
Xenopus tropicalis (tropical clawed frog)	gnas	NP_998855.1
Pan troglodytes (chimpanzee)	GNAS	XP_001138016.3
Drosophila melanogaster (fruit fly)	G-salpha60A	NP_726388.1
Anopheles gambiae (African malaria mosquito)	GNAS_ANOGA	XP_320433.1
Bos taurus (cattle)	GNAS	NP_851364.1
Mus musculus (house mouse)	Gnas	NP_034439.2
Gallus gallus (chicken)	GNAS	XP_417485.3
Homo sapiens (human)	GNAS	NP_536350.2
Danio rerio (zebrafish)	gnas	XP_005172181.1
	GNAS	XP_001083687.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DGsalpha is involved in trehalose perception. We suggest that sugar-taste signals are processed through the Gsalpha-mediating signal transduction pathway in sugar-sensitive GRNs in Drosophila.
Title: Gsalpha is involved in sugar perception in Drosophila melanogaster.

dgsR60 mutant synaptic transmission defects were caused predominantly by lack of Gs(alpha) in presynaptic neurons and not in postsynaptic muscles
Title: Presynaptic impairment of synaptic transmission in Drosophila embryos lacking Gs(alpha).

The following Galphas gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Galphas cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa11082	NM_001169812.2 Latest version!	Drosophila melanogaster G protein alpha s subunit (Galphas), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa11082	NM_058157.5 Latest version!	Drosophila melanogaster G protein alpha s subunit (Galphas), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa13219	NM_166640.3 Latest version!	Drosophila melanogaster G protein alpha s subunit (Galphas), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa13219	NM_058158.5 Latest version!	Drosophila melanogaster G protein alpha s subunit (Galphas), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa13219	NM_001299869.1 Latest version!	Drosophila melanogaster G protein alpha s subunit (Galphas), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Galphas ( NM_058157.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477505.1 Drosophila melanogaster G protein alpha s subunit (Galphas), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa11082
Clone ID Related Accession (Same CDS sequence)	NM_058157.5 , NM_001169812.2
Accession Version	NM_058157.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	G protein alpha s subunit, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_058157.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTTGCT TTGGGTCGCC CACCTCCAAG CAGTCGGACG TGAACTCGGA GGACTCGAAG 
AGCCAGAAGC GCCGGAGCGA TGCAATATCT AGACAGTTGC AGAAGGACAA ACAGCTCTAC 
AGGGCCACAC ACAGGCTGCT CCTCCTGGGG GCGGGCGAGT CCGGCAAATC AACCATAGTC 
AAGCAAATGC GAATATTGCA TGTCGACGGA TTTTCTGACT CGGAAAAGAA ACAGAAAATT 
GATGATATTA AAAAGAATAT TCGAGACGCT ATCTTGACTA TTACAGGAGC CATGAGCACA 
CTTAATCCAC CTGTAGCTTT AGAAAAGAAG GAAAATGAAC CCAGAGTGGA GTACATTCAG 
GATTATGCAT CTAGTCCGGA CTTTAATTAT CCTCCTGAAT TTTATGAACA TACAGAAGAA 
CTATGGAAAG ACAAGGGCGT TCTTCAAACC TATGAGAGGT CGAATGAGTA TCAATTAATC 
GATTGTGCGA AATACTTCCT GGACCGAGTG AGCACAATCA AGAATCCAAA CTACACCCCT 
AATGAGCAGG ATATTCTTCG GTGCCGTGTT TTGACTTCTG GAATATTTGA AACAAGATTT 
CAAGTGGACA AAGTAAACTT TCACATGTTC GATGTCGGTG GCCAGCGGGA CGAGCGTAGG 
AAATGGATTC AGTGTTTCAA TGATGTAACT GCTATCATAT TCGTAACTGC GTGCTCAAGT 
TATAACATGG TTTTGCGGGA AGATCCCACC CAGAACCGAC TTCGAGAATC TTTGGATTTG 
TTCAAGAGTA TTTGGAACAA CAGATGGCTT CGCACGATTT CTATTATACT ATTTTTAAAT 
AAGCAAGATT TGTTAGCAGA GAAAATTAAG GCTGGAAAAA GTAAATTGTC GGAATATTTC 
TCCGAGTTTA ACAAATACCA AACGCCAATC GACACAGGTG ACGCAATAAT GGAATCCAAT 
GATGACCCAG AAGTAATACG AGCAAAATAT TTCATACGAG ACGAGTTTCT GCGTATATCT 
ACCGCTAGCG GAGACGGAAA ACACTACTGC TATCCACATT TCACATGCGC CGTTGACACA 
GAAAACATTA AACGTGTGTT TAATGATTGC AGAGACATTA TTCAAAGGAT GCACCTTCGT 
CAATATGAAT TGTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477505.1
CDS	513..1670
Translation	MGCFGSPTSKQSDVNSEDSKSQKRRSDAISRQLQKDKQLYRATHRLLLLGAGESGKSTIVKQMRILHVDGFSDSEKKQKIDDIKKNIRDAILTITGAMSTLNPPVALEKKENEPRVEYIQDYASSPDFNYPPEFYEHTEELWKDKGVLQTYERSNEYQLIDCAKYFLDRVSTIKNPNYTPNEQDILRCRVLTSGIFETRFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVTACSSYNMVLREDPTQNRLRESLDLFKSIWNNRWLRTISIILFLNKQDLLAEKIKAGKSKLSEYFSEFNKYQTPIDTGDAIMESNDDPEVIRAKYFIRDEFLRISTASGDGKHYCYPHFTCAVDTENIKRVFNDCRDIIQRMHLRQYELL

Target ORF information:

RefSeq Version	NM_058157.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster G protein alpha s subunit (Galphas), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058157.5

ORF Insert Sequence:

ATGGGTTGCT TTGGGTCGCC CACCTCCAAG CAGTCGGACG TGAACTCGGA GGACTCGAAG 
AGCCAGAAGC GCCGGAGCGA TGCAATATCT AGACAGTTGC AGAAGGACAA ACAGCTCTAC 
AGGGCCACAC ACAGGCTGCT CCTCCTGGGG GCGGGCGAGT CCGGCAAATC AACCATAGTC 
AAGCAAATGC GAATATTGCA TGTCGACGGA TTTTCTGACT CGGAAAAGAA ACAGAAAATT 
GATGATATTA AAAAGAATAT TCGAGACGCT ATCTTGACTA TTACAGGAGC CATGAGCACA 
CTTAATCCAC CTGTAGCTTT AGAAAAGAAG GAAAATGAAC CCAGAGTGGA GTACATTCAG 
GATTATGCAT CTAGTCCGGA CTTTAATTAT CCTCCTGAAT TTTATGAACA TACAGAAGAA 
CTATGGAAAG ACAAGGGCGT TCTTCAAACC TATGAGAGGT CGAATGAGTA TCAATTAATC 
GATTGTGCGA AATACTTCCT GGACCGAGTG AGCACAATCA AGAATCCAAA CTACACCCCT 
AATGAGCAGG ATATTCTTCG GTGCCGTGTT TTGACTTCTG GAATATTTGA AACAAGATTT 
CAAGTGGACA AAGTAAACTT TCACATGTTC GATGTCGGTG GCCAGCGGGA CGAGCGTAGG 
AAATGGATTC AGTGTTTCAA TGATGTAACT GCTATCATAT TCGTAACTGC GTGCTCAAGT 
TATAACATGG TTTTGCGGGA AGATCCCACC CAGAACCGAC TTCGAGAATC TTTGGATTTG 
TTCAAGAGTA TTTGGAACAA CAGATGGCTT CGCACGATTT CTATTATACT ATTTTTAAAT 
AAGCAAGATT TGTTAGCAGA GAAAATTAAG GCTGGAAAAA GTAAATTGTC GGAATATTTC 
TCCGAGTTTA ACAAATACCA AACGCCAATC GACACAGGTG ACGCAATAAT GGAATCCAAT 
GATGACCCAG AAGTAATACG AGCAAAATAT TTCATACGAG ACGAGTTTCT GCGTATATCT 
ACCGCTAGCG GAGACGGAAA ACACTACTGC TATCCACATT TCACATGCGC CGTTGACACA 
GAAAACATTA AACGTGTGTT TAATGATTGC AGAGACATTA TTCAAAGGAT GCACCTTCGT 
CAATATGAAT TGTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Galphas ( NM_001169812.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163283.1 Drosophila melanogaster G protein alpha s subunit (Galphas), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa11082
Clone ID Related Accession (Same CDS sequence)	NM_058157.5 , NM_001169812.2
Accession Version	NM_001169812.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	G protein alpha s subunit, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001169812.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTTGCT TTGGGTCGCC CACCTCCAAG CAGTCGGACG TGAACTCGGA GGACTCGAAG 
AGCCAGAAGC GCCGGAGCGA TGCAATATCT AGACAGTTGC AGAAGGACAA ACAGCTCTAC 
AGGGCCACAC ACAGGCTGCT CCTCCTGGGG GCGGGCGAGT CCGGCAAATC AACCATAGTC 
AAGCAAATGC GAATATTGCA TGTCGACGGA TTTTCTGACT CGGAAAAGAA ACAGAAAATT 
GATGATATTA AAAAGAATAT TCGAGACGCT ATCTTGACTA TTACAGGAGC CATGAGCACA 
CTTAATCCAC CTGTAGCTTT AGAAAAGAAG GAAAATGAAC CCAGAGTGGA GTACATTCAG 
GATTATGCAT CTAGTCCGGA CTTTAATTAT CCTCCTGAAT TTTATGAACA TACAGAAGAA 
CTATGGAAAG ACAAGGGCGT TCTTCAAACC TATGAGAGGT CGAATGAGTA TCAATTAATC 
GATTGTGCGA AATACTTCCT GGACCGAGTG AGCACAATCA AGAATCCAAA CTACACCCCT 
AATGAGCAGG ATATTCTTCG GTGCCGTGTT TTGACTTCTG GAATATTTGA AACAAGATTT 
CAAGTGGACA AAGTAAACTT TCACATGTTC GATGTCGGTG GCCAGCGGGA CGAGCGTAGG 
AAATGGATTC AGTGTTTCAA TGATGTAACT GCTATCATAT TCGTAACTGC GTGCTCAAGT 
TATAACATGG TTTTGCGGGA AGATCCCACC CAGAACCGAC TTCGAGAATC TTTGGATTTG 
TTCAAGAGTA TTTGGAACAA CAGATGGCTT CGCACGATTT CTATTATACT ATTTTTAAAT 
AAGCAAGATT TGTTAGCAGA GAAAATTAAG GCTGGAAAAA GTAAATTGTC GGAATATTTC 
TCCGAGTTTA ACAAATACCA AACGCCAATC GACACAGGTG ACGCAATAAT GGAATCCAAT 
GATGACCCAG AAGTAATACG AGCAAAATAT TTCATACGAG ACGAGTTTCT GCGTATATCT 
ACCGCTAGCG GAGACGGAAA ACACTACTGC TATCCACATT TCACATGCGC CGTTGACACA 
GAAAACATTA AACGTGTGTT TAATGATTGC AGAGACATTA TTCAAAGGAT GCACCTTCGT 
CAATATGAAT TGTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163283.1
CDS	300..1457
Translation	MGCFGSPTSKQSDVNSEDSKSQKRRSDAISRQLQKDKQLYRATHRLLLLGAGESGKSTIVKQMRILHVDGFSDSEKKQKIDDIKKNIRDAILTITGAMSTLNPPVALEKKENEPRVEYIQDYASSPDFNYPPEFYEHTEELWKDKGVLQTYERSNEYQLIDCAKYFLDRVSTIKNPNYTPNEQDILRCRVLTSGIFETRFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVTACSSYNMVLREDPTQNRLRESLDLFKSIWNNRWLRTISIILFLNKQDLLAEKIKAGKSKLSEYFSEFNKYQTPIDTGDAIMESNDDPEVIRAKYFIRDEFLRISTASGDGKHYCYPHFTCAVDTENIKRVFNDCRDIIQRMHLRQYELL

Target ORF information:

RefSeq Version	NM_001169812.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster G protein alpha s subunit (Galphas), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169812.2

ORF Insert Sequence:

ATGGGTTGCT TTGGGTCGCC CACCTCCAAG CAGTCGGACG TGAACTCGGA GGACTCGAAG 
AGCCAGAAGC GCCGGAGCGA TGCAATATCT AGACAGTTGC AGAAGGACAA ACAGCTCTAC 
AGGGCCACAC ACAGGCTGCT CCTCCTGGGG GCGGGCGAGT CCGGCAAATC AACCATAGTC 
AAGCAAATGC GAATATTGCA TGTCGACGGA TTTTCTGACT CGGAAAAGAA ACAGAAAATT 
GATGATATTA AAAAGAATAT TCGAGACGCT ATCTTGACTA TTACAGGAGC CATGAGCACA 
CTTAATCCAC CTGTAGCTTT AGAAAAGAAG GAAAATGAAC CCAGAGTGGA GTACATTCAG 
GATTATGCAT CTAGTCCGGA CTTTAATTAT CCTCCTGAAT TTTATGAACA TACAGAAGAA 
CTATGGAAAG ACAAGGGCGT TCTTCAAACC TATGAGAGGT CGAATGAGTA TCAATTAATC 
GATTGTGCGA AATACTTCCT GGACCGAGTG AGCACAATCA AGAATCCAAA CTACACCCCT 
AATGAGCAGG ATATTCTTCG GTGCCGTGTT TTGACTTCTG GAATATTTGA AACAAGATTT 
CAAGTGGACA AAGTAAACTT TCACATGTTC GATGTCGGTG GCCAGCGGGA CGAGCGTAGG 
AAATGGATTC AGTGTTTCAA TGATGTAACT GCTATCATAT TCGTAACTGC GTGCTCAAGT 
TATAACATGG TTTTGCGGGA AGATCCCACC CAGAACCGAC TTCGAGAATC TTTGGATTTG 
TTCAAGAGTA TTTGGAACAA CAGATGGCTT CGCACGATTT CTATTATACT ATTTTTAAAT 
AAGCAAGATT TGTTAGCAGA GAAAATTAAG GCTGGAAAAA GTAAATTGTC GGAATATTTC 
TCCGAGTTTA ACAAATACCA AACGCCAATC GACACAGGTG ACGCAATAAT GGAATCCAAT 
GATGACCCAG AAGTAATACG AGCAAAATAT TTCATACGAG ACGAGTTTCT GCGTATATCT 
ACCGCTAGCG GAGACGGAAA ACACTACTGC TATCCACATT TCACATGCGC CGTTGACACA 
GAAAACATTA AACGTGTGTT TAATGATTGC AGAGACATTA TTCAAAGGAT GCACCTTCGT 
CAATATGAAT TGTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Galphas ( NM_058158.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477506.1 Drosophila melanogaster G protein alpha s subunit (Galphas), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13219
Clone ID Related Accession (Same CDS sequence)	NM_058158.5 , NM_166640.3 , NM_001299869.1
Accession Version	NM_058158.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	G protein alpha s subunit, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_058158.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTTGCT TTGGGTCGCC CACCTCCAAG CAGTCGGACG TGAACTCGGA GGACTCGAAG 
AGCCAGAAGC GCCGGAGCGA TGCAATATCT AGACAGTTGC AGAAGGACAA ACAGCTCTAC 
AGGGCCACAC ACAGGCTGCT CCTCCTGGGG GCGGGCGAGT CCGGCAAATC AACCATAGTC 
AAGCAAATGC GAATATTGCA TGTCGACGGA TTTTCTGACT CGGAAAAGAA ACAGAAAATT 
GATGATATTA AAAAGAATAT TCGAGACGCT ATCTTGACTA TTACAGGAGC CATGAGCACA 
CTTAATCCAC CTGTAGCTTT AGAAAAGAAG GAAAATGAAC CCAGAGTGGA GTACATTCAG 
GATTATGCAT CTAGTCCGGA CTTTAATTAT CCTCCTGAAT TTTATGAACA TACAGAAGAA 
CTATGGAAAG ACAAGGGCGT TCTTCAAACC TATGAGAGGT CGAATGAGTA TCAATTAATC 
GATTGTGCGA AATACTTCCT GGACCGAGTG AGCACAATCA AGAATCCAAA CTACACCCCT 
AATGAGCAGG ATATTCTTCG GTGCCGTGTT TTGACTTCTG GAATATTTGA AACAAGATTT 
CAAGTGGACA AAGTAAACTT TCACATGTTC GATGTCGGTG GCCAGCGGGA CGAGCGTAGG 
AAATGGATTC AGTGTTTCAA TGATGTAACT GCTATCATAT TCGTAACTGC GTGCTCAAGT 
TATAACATGG TTTTGCGGGA AGATCCCACC CAGAACCGAC TTCGAGAATC TTTGGATTTG 
TTCAAGAGTA TTTGGAACAA CAGATGGCTT CGCACGATTT CTATTATACT ATTTTTAAAT 
AAGCAAGATT TGTTAGCAGA GAAAATTAAG GCTGGAAAAA GTAAATTGTC GGAATATTTC 
TCCGAGTTTA ACAAATACCA AACGCCAAGT GACGCAATAA TGGAATCCAA TGATGACCCA 
GAAGTAATAC GAGCAAAATA TTTCATACGA GACGAGTTTC TGCGTATATC TACCGCTAGC 
GGAGACGGAA AACACTACTG CTATCCACAT TTCACATGCG CCGTTGACAC AGAAAACATT 
AAACGTGTGT TTAATGATTG CAGAGACATT ATTCAAAGGA TGCACCTTCG TCAATATGAA 
TTGTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477506.1
CDS	513..1661
Translation	MGCFGSPTSKQSDVNSEDSKSQKRRSDAISRQLQKDKQLYRATHRLLLLGAGESGKSTIVKQMRILHVDGFSDSEKKQKIDDIKKNIRDAILTITGAMSTLNPPVALEKKENEPRVEYIQDYASSPDFNYPPEFYEHTEELWKDKGVLQTYERSNEYQLIDCAKYFLDRVSTIKNPNYTPNEQDILRCRVLTSGIFETRFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVTACSSYNMVLREDPTQNRLRESLDLFKSIWNNRWLRTISIILFLNKQDLLAEKIKAGKSKLSEYFSEFNKYQTPSDAIMESNDDPEVIRAKYFIRDEFLRISTASGDGKHYCYPHFTCAVDTENIKRVFNDCRDIIQRMHLRQYELL

Target ORF information:

RefSeq Version	NM_058158.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster G protein alpha s subunit (Galphas), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058158.5

ORF Insert Sequence:

ATGGGTTGCT TTGGGTCGCC CACCTCCAAG CAGTCGGACG TGAACTCGGA GGACTCGAAG 
AGCCAGAAGC GCCGGAGCGA TGCAATATCT AGACAGTTGC AGAAGGACAA ACAGCTCTAC 
AGGGCCACAC ACAGGCTGCT CCTCCTGGGG GCGGGCGAGT CCGGCAAATC AACCATAGTC 
AAGCAAATGC GAATATTGCA TGTCGACGGA TTTTCTGACT CGGAAAAGAA ACAGAAAATT 
GATGATATTA AAAAGAATAT TCGAGACGCT ATCTTGACTA TTACAGGAGC CATGAGCACA 
CTTAATCCAC CTGTAGCTTT AGAAAAGAAG GAAAATGAAC CCAGAGTGGA GTACATTCAG 
GATTATGCAT CTAGTCCGGA CTTTAATTAT CCTCCTGAAT TTTATGAACA TACAGAAGAA 
CTATGGAAAG ACAAGGGCGT TCTTCAAACC TATGAGAGGT CGAATGAGTA TCAATTAATC 
GATTGTGCGA AATACTTCCT GGACCGAGTG AGCACAATCA AGAATCCAAA CTACACCCCT 
AATGAGCAGG ATATTCTTCG GTGCCGTGTT TTGACTTCTG GAATATTTGA AACAAGATTT 
CAAGTGGACA AAGTAAACTT TCACATGTTC GATGTCGGTG GCCAGCGGGA CGAGCGTAGG 
AAATGGATTC AGTGTTTCAA TGATGTAACT GCTATCATAT TCGTAACTGC GTGCTCAAGT 
TATAACATGG TTTTGCGGGA AGATCCCACC CAGAACCGAC TTCGAGAATC TTTGGATTTG 
TTCAAGAGTA TTTGGAACAA CAGATGGCTT CGCACGATTT CTATTATACT ATTTTTAAAT 
AAGCAAGATT TGTTAGCAGA GAAAATTAAG GCTGGAAAAA GTAAATTGTC GGAATATTTC 
TCCGAGTTTA ACAAATACCA AACGCCAAGT GACGCAATAA TGGAATCCAA TGATGACCCA 
GAAGTAATAC GAGCAAAATA TTTCATACGA GACGAGTTTC TGCGTATATC TACCGCTAGC 
GGAGACGGAA AACACTACTG CTATCCACAT TTCACATGCG CCGTTGACAC AGAAAACATT 
AAACGTGTGT TTAATGATTG CAGAGACATT ATTCAAAGGA TGCACCTTCG TCAATATGAA 
TTGTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Galphas ( NM_166640.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726388.1 Drosophila melanogaster G protein alpha s subunit (Galphas), transcript variant C, mRNA.

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CloneID	OFa13219
Clone ID Related Accession (Same CDS sequence)	NM_058158.5 , NM_166640.3 , NM_001299869.1
Accession Version	NM_166640.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	G protein alpha s subunit, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166640.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTTGCT TTGGGTCGCC CACCTCCAAG CAGTCGGACG TGAACTCGGA GGACTCGAAG 
AGCCAGAAGC GCCGGAGCGA TGCAATATCT AGACAGTTGC AGAAGGACAA ACAGCTCTAC 
AGGGCCACAC ACAGGCTGCT CCTCCTGGGG GCGGGCGAGT CCGGCAAATC AACCATAGTC 
AAGCAAATGC GAATATTGCA TGTCGACGGA TTTTCTGACT CGGAAAAGAA ACAGAAAATT 
GATGATATTA AAAAGAATAT TCGAGACGCT ATCTTGACTA TTACAGGAGC CATGAGCACA 
CTTAATCCAC CTGTAGCTTT AGAAAAGAAG GAAAATGAAC CCAGAGTGGA GTACATTCAG 
GATTATGCAT CTAGTCCGGA CTTTAATTAT CCTCCTGAAT TTTATGAACA TACAGAAGAA 
CTATGGAAAG ACAAGGGCGT TCTTCAAACC TATGAGAGGT CGAATGAGTA TCAATTAATC 
GATTGTGCGA AATACTTCCT GGACCGAGTG AGCACAATCA AGAATCCAAA CTACACCCCT 
AATGAGCAGG ATATTCTTCG GTGCCGTGTT TTGACTTCTG GAATATTTGA AACAAGATTT 
CAAGTGGACA AAGTAAACTT TCACATGTTC GATGTCGGTG GCCAGCGGGA CGAGCGTAGG 
AAATGGATTC AGTGTTTCAA TGATGTAACT GCTATCATAT TCGTAACTGC GTGCTCAAGT 
TATAACATGG TTTTGCGGGA AGATCCCACC CAGAACCGAC TTCGAGAATC TTTGGATTTG 
TTCAAGAGTA TTTGGAACAA CAGATGGCTT CGCACGATTT CTATTATACT ATTTTTAAAT 
AAGCAAGATT TGTTAGCAGA GAAAATTAAG GCTGGAAAAA GTAAATTGTC GGAATATTTC 
TCCGAGTTTA ACAAATACCA AACGCCAAGT GACGCAATAA TGGAATCCAA TGATGACCCA 
GAAGTAATAC GAGCAAAATA TTTCATACGA GACGAGTTTC TGCGTATATC TACCGCTAGC 
GGAGACGGAA AACACTACTG CTATCCACAT TTCACATGCG CCGTTGACAC AGAAAACATT 
AAACGTGTGT TTAATGATTG CAGAGACATT ATTCAAAGGA TGCACCTTCG TCAATATGAA 
TTGTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726388.1
CDS	300..1448
Translation	MGCFGSPTSKQSDVNSEDSKSQKRRSDAISRQLQKDKQLYRATHRLLLLGAGESGKSTIVKQMRILHVDGFSDSEKKQKIDDIKKNIRDAILTITGAMSTLNPPVALEKKENEPRVEYIQDYASSPDFNYPPEFYEHTEELWKDKGVLQTYERSNEYQLIDCAKYFLDRVSTIKNPNYTPNEQDILRCRVLTSGIFETRFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVTACSSYNMVLREDPTQNRLRESLDLFKSIWNNRWLRTISIILFLNKQDLLAEKIKAGKSKLSEYFSEFNKYQTPSDAIMESNDDPEVIRAKYFIRDEFLRISTASGDGKHYCYPHFTCAVDTENIKRVFNDCRDIIQRMHLRQYELL

Target ORF information:

RefSeq Version	NM_166640.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster G protein alpha s subunit (Galphas), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166640.3

ORF Insert Sequence:

ATGGGTTGCT TTGGGTCGCC CACCTCCAAG CAGTCGGACG TGAACTCGGA GGACTCGAAG 
AGCCAGAAGC GCCGGAGCGA TGCAATATCT AGACAGTTGC AGAAGGACAA ACAGCTCTAC 
AGGGCCACAC ACAGGCTGCT CCTCCTGGGG GCGGGCGAGT CCGGCAAATC AACCATAGTC 
AAGCAAATGC GAATATTGCA TGTCGACGGA TTTTCTGACT CGGAAAAGAA ACAGAAAATT 
GATGATATTA AAAAGAATAT TCGAGACGCT ATCTTGACTA TTACAGGAGC CATGAGCACA 
CTTAATCCAC CTGTAGCTTT AGAAAAGAAG GAAAATGAAC CCAGAGTGGA GTACATTCAG 
GATTATGCAT CTAGTCCGGA CTTTAATTAT CCTCCTGAAT TTTATGAACA TACAGAAGAA 
CTATGGAAAG ACAAGGGCGT TCTTCAAACC TATGAGAGGT CGAATGAGTA TCAATTAATC 
GATTGTGCGA AATACTTCCT GGACCGAGTG AGCACAATCA AGAATCCAAA CTACACCCCT 
AATGAGCAGG ATATTCTTCG GTGCCGTGTT TTGACTTCTG GAATATTTGA AACAAGATTT 
CAAGTGGACA AAGTAAACTT TCACATGTTC GATGTCGGTG GCCAGCGGGA CGAGCGTAGG 
AAATGGATTC AGTGTTTCAA TGATGTAACT GCTATCATAT TCGTAACTGC GTGCTCAAGT 
TATAACATGG TTTTGCGGGA AGATCCCACC CAGAACCGAC TTCGAGAATC TTTGGATTTG 
TTCAAGAGTA TTTGGAACAA CAGATGGCTT CGCACGATTT CTATTATACT ATTTTTAAAT 
AAGCAAGATT TGTTAGCAGA GAAAATTAAG GCTGGAAAAA GTAAATTGTC GGAATATTTC 
TCCGAGTTTA ACAAATACCA AACGCCAAGT GACGCAATAA TGGAATCCAA TGATGACCCA 
GAAGTAATAC GAGCAAAATA TTTCATACGA GACGAGTTTC TGCGTATATC TACCGCTAGC 
GGAGACGGAA AACACTACTG CTATCCACAT TTCACATGCG CCGTTGACAC AGAAAACATT 
AAACGTGTGT TTAATGATTG CAGAGACATT ATTCAAAGGA TGCACCTTCG TCAATATGAA 
TTGTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Galphas ( NM_001299869.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286798.1 Drosophila melanogaster G protein alpha s subunit (Galphas), transcript variant E, mRNA.

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CloneID	OFa13219
Clone ID Related Accession (Same CDS sequence)	NM_058158.5 , NM_166640.3 , NM_001299869.1
Accession Version	NM_001299869.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	G protein alpha s subunit, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTTGCT TTGGGTCGCC CACCTCCAAG CAGTCGGACG TGAACTCGGA GGACTCGAAG 
AGCCAGAAGC GCCGGAGCGA TGCAATATCT AGACAGTTGC AGAAGGACAA ACAGCTCTAC 
AGGGCCACAC ACAGGCTGCT CCTCCTGGGG GCGGGCGAGT CCGGCAAATC AACCATAGTC 
AAGCAAATGC GAATATTGCA TGTCGACGGA TTTTCTGACT CGGAAAAGAA ACAGAAAATT 
GATGATATTA AAAAGAATAT TCGAGACGCT ATCTTGACTA TTACAGGAGC CATGAGCACA 
CTTAATCCAC CTGTAGCTTT AGAAAAGAAG GAAAATGAAC CCAGAGTGGA GTACATTCAG 
GATTATGCAT CTAGTCCGGA CTTTAATTAT CCTCCTGAAT TTTATGAACA TACAGAAGAA 
CTATGGAAAG ACAAGGGCGT TCTTCAAACC TATGAGAGGT CGAATGAGTA TCAATTAATC 
GATTGTGCGA AATACTTCCT GGACCGAGTG AGCACAATCA AGAATCCAAA CTACACCCCT 
AATGAGCAGG ATATTCTTCG GTGCCGTGTT TTGACTTCTG GAATATTTGA AACAAGATTT 
CAAGTGGACA AAGTAAACTT TCACATGTTC GATGTCGGTG GCCAGCGGGA CGAGCGTAGG 
AAATGGATTC AGTGTTTCAA TGATGTAACT GCTATCATAT TCGTAACTGC GTGCTCAAGT 
TATAACATGG TTTTGCGGGA AGATCCCACC CAGAACCGAC TTCGAGAATC TTTGGATTTG 
TTCAAGAGTA TTTGGAACAA CAGATGGCTT CGCACGATTT CTATTATACT ATTTTTAAAT 
AAGCAAGATT TGTTAGCAGA GAAAATTAAG GCTGGAAAAA GTAAATTGTC GGAATATTTC 
TCCGAGTTTA ACAAATACCA AACGCCAAGT GACGCAATAA TGGAATCCAA TGATGACCCA 
GAAGTAATAC GAGCAAAATA TTTCATACGA GACGAGTTTC TGCGTATATC TACCGCTAGC 
GGAGACGGAA AACACTACTG CTATCCACAT TTCACATGCG CCGTTGACAC AGAAAACATT 
AAACGTGTGT TTAATGATTG CAGAGACATT ATTCAAAGGA TGCACCTTCG TCAATATGAA 
TTGTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286798.1
CDS	489..1637
Translation	MGCFGSPTSKQSDVNSEDSKSQKRRSDAISRQLQKDKQLYRATHRLLLLGAGESGKSTIVKQMRILHVDGFSDSEKKQKIDDIKKNIRDAILTITGAMSTLNPPVALEKKENEPRVEYIQDYASSPDFNYPPEFYEHTEELWKDKGVLQTYERSNEYQLIDCAKYFLDRVSTIKNPNYTPNEQDILRCRVLTSGIFETRFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVTACSSYNMVLREDPTQNRLRESLDLFKSIWNNRWLRTISIILFLNKQDLLAEKIKAGKSKLSEYFSEFNKYQTPSDAIMESNDDPEVIRAKYFIRDEFLRISTASGDGKHYCYPHFTCAVDTENIKRVFNDCRDIIQRMHLRQYELL

Target ORF information:

RefSeq Version	NM_001299869.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster G protein alpha s subunit (Galphas), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299869.1

ORF Insert Sequence:

ATGGGTTGCT TTGGGTCGCC CACCTCCAAG CAGTCGGACG TGAACTCGGA GGACTCGAAG 
AGCCAGAAGC GCCGGAGCGA TGCAATATCT AGACAGTTGC AGAAGGACAA ACAGCTCTAC 
AGGGCCACAC ACAGGCTGCT CCTCCTGGGG GCGGGCGAGT CCGGCAAATC AACCATAGTC 
AAGCAAATGC GAATATTGCA TGTCGACGGA TTTTCTGACT CGGAAAAGAA ACAGAAAATT 
GATGATATTA AAAAGAATAT TCGAGACGCT ATCTTGACTA TTACAGGAGC CATGAGCACA 
CTTAATCCAC CTGTAGCTTT AGAAAAGAAG GAAAATGAAC CCAGAGTGGA GTACATTCAG 
GATTATGCAT CTAGTCCGGA CTTTAATTAT CCTCCTGAAT TTTATGAACA TACAGAAGAA 
CTATGGAAAG ACAAGGGCGT TCTTCAAACC TATGAGAGGT CGAATGAGTA TCAATTAATC 
GATTGTGCGA AATACTTCCT GGACCGAGTG AGCACAATCA AGAATCCAAA CTACACCCCT 
AATGAGCAGG ATATTCTTCG GTGCCGTGTT TTGACTTCTG GAATATTTGA AACAAGATTT 
CAAGTGGACA AAGTAAACTT TCACATGTTC GATGTCGGTG GCCAGCGGGA CGAGCGTAGG 
AAATGGATTC AGTGTTTCAA TGATGTAACT GCTATCATAT TCGTAACTGC GTGCTCAAGT 
TATAACATGG TTTTGCGGGA AGATCCCACC CAGAACCGAC TTCGAGAATC TTTGGATTTG 
TTCAAGAGTA TTTGGAACAA CAGATGGCTT CGCACGATTT CTATTATACT ATTTTTAAAT 
AAGCAAGATT TGTTAGCAGA GAAAATTAAG GCTGGAAAAA GTAAATTGTC GGAATATTTC 
TCCGAGTTTA ACAAATACCA AACGCCAAGT GACGCAATAA TGGAATCCAA TGATGACCCA 
GAAGTAATAC GAGCAAAATA TTTCATACGA GACGAGTTTC TGCGTATATC TACCGCTAGC 
GGAGACGGAA AACACTACTG CTATCCACAT TTCACATGCG CCGTTGACAC AGAAAACATT 
AAACGTGTGT TTAATGATTG CAGAGACATT ATTCAAAGGA TGCACCTTCG TCAATATGAA 
TTGTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Galphas cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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