Nap1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nap1
Entrez Gene ID	37798
Full Name	Nucleosome assembly protein 1
Synonyms	CG5330,Dmel\CG5330,Kette,NAP-1,NAP1,Nap-1,dNAP-1,dNAP1,dNap1,nap-1,nap1,p56/dCAF-4
General protein information	Preferred Names Nucleosome assembly protein 1 Names CG5330 gene product from transcript CG5330-RA CG5330-PA CG5330-PB CG5330-PC Nap1-PA Nap1-PB Nap1-PC nucleosome assembly protein 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 60A9-60A9

mRNA and Protein(s)

mRNA	Protein	Name
NM_057780.4	NP_477128.1	nucleosome assembly protein 1, isoform A
NM_001259557.2	NP_001246486.1	nucleosome assembly protein 1, isoform C
NM_001259556.1	NP_001246485.1	nucleosome assembly protein 1, isoform B

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Nap1l1	NP_056596.1
Eremothecium gossypii	AGOS_AEL109W	NP_984752.2
Schizosaccharomyces pombe (fission yeast)	nap1	NP_587838.1
Neurospora crassa	NCU01438	XP_955998.2
Arabidopsis thaliana (thale cress)	NAP1;2	NP_179538.1
Arabidopsis thaliana (thale cress)	NAP1;1	NP_194341.1
Oryza sativa (rice)	Os05g0539700	NP_001056175.1
Xenopus tropicalis (tropical clawed frog)	nap1l1	NP_001015984.1
Gallus gallus (chicken)	NAP1L1	XP_416106.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001928	XP_321135.5
Magnaporthe oryzae (rice blast fungus)	MGG_06924	XP_003709662.1
Pan troglodytes (chimpanzee)	NAP1L1	XP_509226.2
	NAP1L1	XP_001103652.1
Saccharomyces cerevisiae (baker's yeast)	NAP1	NP_012974.1
Oryza sativa (rice)	Os06g0149400	NP_001056816.1
Homo sapiens (human)	NAP1L1	NP_631946.1
Canis lupus familiaris (dog)	NAP1L1	XP_005629221.1
Rattus norvegicus (Norway rat)	Nap1l1	NP_446013.2
Drosophila melanogaster (fruit fly)	Nap1	NP_001246485.1
Kluyveromyces lactis	KLLA0E15049g	XP_454628.1
Bos taurus (cattle)	NAP1L1	NP_001092685.1
Danio rerio (zebrafish)	nap1l1	NP_958475.1
Arabidopsis thaliana (thale cress)	NAP1;3	NP_568844.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

show that NAP-1 regulates the transcription of the HeT-A retrotransposon, pointing to a positive regulatory role of NAP-1 in telomere expression
Title: NAP-1, Nucleosome assembly protein 1, a histone chaperone involved in Drosophila telomeres.

We find that the assembly of H3.3-containing tetrasomes and nucleosomes by the histone chaperone Nucleosome Assembly Protein 1 (NAP1) occurs in an identical manner to that of H3-containing tetrasomes and nucleosomes.
Title: Comparing the Assembly and Handedness Dynamics of (H3.3-H4)2 Tetrasomes to Canonical Tetrasomes.

Data indicate the essential roles of two Nap family proteins, CG5017/Hanabi and nucleosome assembly protein 1 (Nap1) in spermatogenesis.
Title: The Nap family proteins, CG5017/Hanabi and Nap1, are essential for Drosophila spermiogenesis.

We conclude that the balanced antagonism between NAP1 and PP2A controls cohesin dissociation during mitosis
Title: Histone chaperone NAP1 mediates sister chromatid resolution by counteracting protein phosphatase 2A.

The Drosophila Hem/Kette/Nap1 protein regulates asymmetric division of neural precursor cells by regulating localization of Inscuteable and Numb.
Title: The Drosophila Hem/Kette/Nap1 protein regulates asymmetric division of neural precursor cells by regulating localization of Inscuteable and Numb.

The following Nap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa11081	NM_001259556.1 Latest version!	Drosophila melanogaster nucleosome assembly protein 1, transcript variant B (Nap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa11081	NM_001259557.2 Latest version!	Drosophila melanogaster nucleosome assembly protein 1, transcript variant C (Nap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa11081	NM_057780.4 Latest version!	Drosophila melanogaster nucleosome assembly protein 1, transcript variant A (Nap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nap1 ( NM_057780.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477128.1 Drosophila melanogaster nucleosome assembly protein 1, transcript variant A (Nap1), mRNA.

Price & Availability↑

CloneID	OFa11081
Clone ID Related Accession (Same CDS sequence)	NM_057780.4 , NM_001259557.2 , NM_001259556.1
Accession Version	NM_057780.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nucleosome assembly protein 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057780.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGCCC CAGCCGAAGG ACACGTTGAC CCCGAGTCCT GCAACGAAAT CGAGGACGAG 
AAGTCCGGCT CGGACTGCCA GTCGATGCCC GCCTACATGA ACTCGGTCAT GCGGCGACAG 
TATCTGCAGC AAATGGTCAA GATGCTGCCG GCTCCAGTGC AGAATCGGAT TGTGTTCCTG 
AAAAACCTAC AGCTGCAGCA CCTGAATATC GAGGCCCAGT TCTTTGAGGA CGTCTACAAG 
CTGGAGCAGA AGTACCAGGT GCAGTACCAG CCGTTGTTCG ATAAGCGCAG GGAGATCATC 
GAGGGCAAGG TGGACCCCGC CGAGGAGAAG CCCCAGTGGA AGGAACCGGA GTCGTCGACC 
GACAACGAGG CCGATGCCGA GCACTTCCGC GAGGCCCTGA GCAGTTTGAA AAGTATTCCC 
AAGGATGCCA AGGGCATTCC CGGCTTCTGG CTGACGGTGT TCCGCAACAC GGCCATAATG 
TCTGAGATGG TGCAGCCGCA CGACGAGCCC GCCATCCGCA AGCTAATCGA TATTTCCATC 
AAGTACGACA ACGGGCATTC GTACACCTTG GAGTTTCACT TCGACAAGAA CGAGTACTTC 
TCCAACTCAG TTCTCACAAA GCAGTACGTT TTGAAGTCCA CCGTGGATCC CAACGATCCG 
TTCGCATTTG AAGGCCCAGA GATCTACAAG TGCACGGGGT GCACCATTAA CTGGGAGAAG 
AAGATGAACC TTACTGTGAA GACCATCCGC AAGAAGCAGA AGCACAAGGA GCGCGGCGCA 
GTGCGCACCA TTGTGAAGCA GGTTCCGACG GATTCCTTCT TCAATTTCTT CAGCCCACCA 
GAGGTCCCGA GCGACCAGGA GGAAGTCGAC GACGACTCCC AGCAGATCCT GGCCACCGAC 
TTCGAAATTG GTCACTTCTT GCGCGCCAGA ATTATTCCAA AAGCAGTGCT TTACTACACT 
GGCGACATCG TCGACGATGA GGACGATGAG GACGAAGAGG AGTATGACGA GAACGAGGAG 
GATGAGTACG ACGACGACGA TGCACCGCCA CCAAAGGGCC CCAAATCAGC CGGTATCAAG 
AAGCAGTCGC CCAACGACTG CCCGAATCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477128.1
CDS	110..1222
Translation	MDAPAEGHVDPESCNEIEDEKSGSDCQSMPAYMNSVMRRQYLQQMVKMLPAPVQNRIVFLKNLQLQHLNIEAQFFEDVYKLEQKYQVQYQPLFDKRREIIEGKVDPAEEKPQWKEPESSTDNEADAEHFREALSSLKSIPKDAKGIPGFWLTVFRNTAIMSEMVQPHDEPAIRKLIDISIKYDNGHSYTLEFHFDKNEYFSNSVLTKQYVLKSTVDPNDPFAFEGPEIYKCTGCTINWEKKMNLTVKTIRKKQKHKERGAVRTIVKQVPTDSFFNFFSPPEVPSDQEEVDDDSQQILATDFEIGHFLRARIIPKAVLYYTGDIVDDEDDEDEEEYDENEEDEYDDDDAPPPKGPKSAGIKKQSPNDCPNQ

Target ORF information:

RefSeq Version	NM_057780.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nucleosome assembly protein 1, transcript variant A (Nap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057780.4

ORF Insert Sequence:

ATGGACGCCC CAGCCGAAGG ACACGTTGAC CCCGAGTCCT GCAACGAAAT CGAGGACGAG 
AAGTCCGGCT CGGACTGCCA GTCGATGCCC GCCTACATGA ACTCGGTCAT GCGGCGACAG 
TATCTGCAGC AAATGGTCAA GATGCTGCCG GCTCCAGTGC AGAATCGGAT TGTGTTCCTG 
AAAAACCTAC AGCTGCAGCA CCTGAATATC GAGGCCCAGT TCTTTGAGGA CGTCTACAAG 
CTGGAGCAGA AGTACCAGGT GCAGTACCAG CCGTTGTTCG ATAAGCGCAG GGAGATCATC 
GAGGGCAAGG TGGACCCCGC CGAGGAGAAG CCCCAGTGGA AGGAACCGGA GTCGTCGACC 
GACAACGAGG CCGATGCCGA GCACTTCCGC GAGGCCCTGA GCAGTTTGAA AAGTATTCCC 
AAGGATGCCA AGGGCATTCC CGGCTTCTGG CTGACGGTGT TCCGCAACAC GGCCATAATG 
TCTGAGATGG TGCAGCCGCA CGACGAGCCC GCCATCCGCA AGCTAATCGA TATTTCCATC 
AAGTACGACA ACGGGCATTC GTACACCTTG GAGTTTCACT TCGACAAGAA CGAGTACTTC 
TCCAACTCAG TTCTCACAAA GCAGTACGTT TTGAAGTCCA CCGTGGATCC CAACGATCCG 
TTCGCATTTG AAGGCCCAGA GATCTACAAG TGCACGGGGT GCACCATTAA CTGGGAGAAG 
AAGATGAACC TTACTGTGAA GACCATCCGC AAGAAGCAGA AGCACAAGGA GCGCGGCGCA 
GTGCGCACCA TTGTGAAGCA GGTTCCGACG GATTCCTTCT TCAATTTCTT CAGCCCACCA 
GAGGTCCCGA GCGACCAGGA GGAAGTCGAC GACGACTCCC AGCAGATCCT GGCCACCGAC 
TTCGAAATTG GTCACTTCTT GCGCGCCAGA ATTATTCCAA AAGCAGTGCT TTACTACACT 
GGCGACATCG TCGACGATGA GGACGATGAG GACGAAGAGG AGTATGACGA GAACGAGGAG 
GATGAGTACG ACGACGACGA TGCACCGCCA CCAAAGGGCC CCAAATCAGC CGGTATCAAG 
AAGCAGTCGC CCAACGACTG CCCGAATCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nap1 ( NM_001259557.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246486.1 Drosophila melanogaster nucleosome assembly protein 1, transcript variant C (Nap1), mRNA.

Price & Availability↑

CloneID	OFa11081
Clone ID Related Accession (Same CDS sequence)	NM_057780.4 , NM_001259557.2 , NM_001259556.1
Accession Version	NM_001259557.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nucleosome assembly protein 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001259557.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGCCC CAGCCGAAGG ACACGTTGAC CCCGAGTCCT GCAACGAAAT CGAGGACGAG 
AAGTCCGGCT CGGACTGCCA GTCGATGCCC GCCTACATGA ACTCGGTCAT GCGGCGACAG 
TATCTGCAGC AAATGGTCAA GATGCTGCCG GCTCCAGTGC AGAATCGGAT TGTGTTCCTG 
AAAAACCTAC AGCTGCAGCA CCTGAATATC GAGGCCCAGT TCTTTGAGGA CGTCTACAAG 
CTGGAGCAGA AGTACCAGGT GCAGTACCAG CCGTTGTTCG ATAAGCGCAG GGAGATCATC 
GAGGGCAAGG TGGACCCCGC CGAGGAGAAG CCCCAGTGGA AGGAACCGGA GTCGTCGACC 
GACAACGAGG CCGATGCCGA GCACTTCCGC GAGGCCCTGA GCAGTTTGAA AAGTATTCCC 
AAGGATGCCA AGGGCATTCC CGGCTTCTGG CTGACGGTGT TCCGCAACAC GGCCATAATG 
TCTGAGATGG TGCAGCCGCA CGACGAGCCC GCCATCCGCA AGCTAATCGA TATTTCCATC 
AAGTACGACA ACGGGCATTC GTACACCTTG GAGTTTCACT TCGACAAGAA CGAGTACTTC 
TCCAACTCAG TTCTCACAAA GCAGTACGTT TTGAAGTCCA CCGTGGATCC CAACGATCCG 
TTCGCATTTG AAGGCCCAGA GATCTACAAG TGCACGGGGT GCACCATTAA CTGGGAGAAG 
AAGATGAACC TTACTGTGAA GACCATCCGC AAGAAGCAGA AGCACAAGGA GCGCGGCGCA 
GTGCGCACCA TTGTGAAGCA GGTTCCGACG GATTCCTTCT TCAATTTCTT CAGCCCACCA 
GAGGTCCCGA GCGACCAGGA GGAAGTCGAC GACGACTCCC AGCAGATCCT GGCCACCGAC 
TTCGAAATTG GTCACTTCTT GCGCGCCAGA ATTATTCCAA AAGCAGTGCT TTACTACACT 
GGCGACATCG TCGACGATGA GGACGATGAG GACGAAGAGG AGTATGACGA GAACGAGGAG 
GATGAGTACG ACGACGACGA TGCACCGCCA CCAAAGGGCC CCAAATCAGC CGGTATCAAG 
AAGCAGTCGC CCAACGACTG CCCGAATCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246486.1
CDS	125..1237
Translation	MDAPAEGHVDPESCNEIEDEKSGSDCQSMPAYMNSVMRRQYLQQMVKMLPAPVQNRIVFLKNLQLQHLNIEAQFFEDVYKLEQKYQVQYQPLFDKRREIIEGKVDPAEEKPQWKEPESSTDNEADAEHFREALSSLKSIPKDAKGIPGFWLTVFRNTAIMSEMVQPHDEPAIRKLIDISIKYDNGHSYTLEFHFDKNEYFSNSVLTKQYVLKSTVDPNDPFAFEGPEIYKCTGCTINWEKKMNLTVKTIRKKQKHKERGAVRTIVKQVPTDSFFNFFSPPEVPSDQEEVDDDSQQILATDFEIGHFLRARIIPKAVLYYTGDIVDDEDDEDEEEYDENEEDEYDDDDAPPPKGPKSAGIKKQSPNDCPNQ

Target ORF information:

RefSeq Version	NM_001259557.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nucleosome assembly protein 1, transcript variant C (Nap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259557.2

ORF Insert Sequence:

ATGGACGCCC CAGCCGAAGG ACACGTTGAC CCCGAGTCCT GCAACGAAAT CGAGGACGAG 
AAGTCCGGCT CGGACTGCCA GTCGATGCCC GCCTACATGA ACTCGGTCAT GCGGCGACAG 
TATCTGCAGC AAATGGTCAA GATGCTGCCG GCTCCAGTGC AGAATCGGAT TGTGTTCCTG 
AAAAACCTAC AGCTGCAGCA CCTGAATATC GAGGCCCAGT TCTTTGAGGA CGTCTACAAG 
CTGGAGCAGA AGTACCAGGT GCAGTACCAG CCGTTGTTCG ATAAGCGCAG GGAGATCATC 
GAGGGCAAGG TGGACCCCGC CGAGGAGAAG CCCCAGTGGA AGGAACCGGA GTCGTCGACC 
GACAACGAGG CCGATGCCGA GCACTTCCGC GAGGCCCTGA GCAGTTTGAA AAGTATTCCC 
AAGGATGCCA AGGGCATTCC CGGCTTCTGG CTGACGGTGT TCCGCAACAC GGCCATAATG 
TCTGAGATGG TGCAGCCGCA CGACGAGCCC GCCATCCGCA AGCTAATCGA TATTTCCATC 
AAGTACGACA ACGGGCATTC GTACACCTTG GAGTTTCACT TCGACAAGAA CGAGTACTTC 
TCCAACTCAG TTCTCACAAA GCAGTACGTT TTGAAGTCCA CCGTGGATCC CAACGATCCG 
TTCGCATTTG AAGGCCCAGA GATCTACAAG TGCACGGGGT GCACCATTAA CTGGGAGAAG 
AAGATGAACC TTACTGTGAA GACCATCCGC AAGAAGCAGA AGCACAAGGA GCGCGGCGCA 
GTGCGCACCA TTGTGAAGCA GGTTCCGACG GATTCCTTCT TCAATTTCTT CAGCCCACCA 
GAGGTCCCGA GCGACCAGGA GGAAGTCGAC GACGACTCCC AGCAGATCCT GGCCACCGAC 
TTCGAAATTG GTCACTTCTT GCGCGCCAGA ATTATTCCAA AAGCAGTGCT TTACTACACT 
GGCGACATCG TCGACGATGA GGACGATGAG GACGAAGAGG AGTATGACGA GAACGAGGAG 
GATGAGTACG ACGACGACGA TGCACCGCCA CCAAAGGGCC CCAAATCAGC CGGTATCAAG 
AAGCAGTCGC CCAACGACTG CCCGAATCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nap1 ( NM_001259556.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246485.1 Drosophila melanogaster nucleosome assembly protein 1, transcript variant B (Nap1), mRNA.

Price & Availability↑

CloneID	OFa11081
Clone ID Related Accession (Same CDS sequence)	NM_057780.4 , NM_001259557.2 , NM_001259556.1
Accession Version	NM_001259556.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nucleosome assembly protein 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACGCCC CAGCCGAAGG ACACGTTGAC CCCGAGTCCT GCAACGAAAT CGAGGACGAG 
AAGTCCGGCT CGGACTGCCA GTCGATGCCC GCCTACATGA ACTCGGTCAT GCGGCGACAG 
TATCTGCAGC AAATGGTCAA GATGCTGCCG GCTCCAGTGC AGAATCGGAT TGTGTTCCTG 
AAAAACCTAC AGCTGCAGCA CCTGAATATC GAGGCCCAGT TCTTTGAGGA CGTCTACAAG 
CTGGAGCAGA AGTACCAGGT GCAGTACCAG CCGTTGTTCG ATAAGCGCAG GGAGATCATC 
GAGGGCAAGG TGGACCCCGC CGAGGAGAAG CCCCAGTGGA AGGAACCGGA GTCGTCGACC 
GACAACGAGG CCGATGCCGA GCACTTCCGC GAGGCCCTGA GCAGTTTGAA AAGTATTCCC 
AAGGATGCCA AGGGCATTCC CGGCTTCTGG CTGACGGTGT TCCGCAACAC GGCCATAATG 
TCTGAGATGG TGCAGCCGCA CGACGAGCCC GCCATCCGCA AGCTAATCGA TATTTCCATC 
AAGTACGACA ACGGGCATTC GTACACCTTG GAGTTTCACT TCGACAAGAA CGAGTACTTC 
TCCAACTCAG TTCTCACAAA GCAGTACGTT TTGAAGTCCA CCGTGGATCC CAACGATCCG 
TTCGCATTTG AAGGCCCAGA GATCTACAAG TGCACGGGGT GCACCATTAA CTGGGAGAAG 
AAGATGAACC TTACTGTGAA GACCATCCGC AAGAAGCAGA AGCACAAGGA GCGCGGCGCA 
GTGCGCACCA TTGTGAAGCA GGTTCCGACG GATTCCTTCT TCAATTTCTT CAGCCCACCA 
GAGGTCCCGA GCGACCAGGA GGAAGTCGAC GACGACTCCC AGCAGATCCT GGCCACCGAC 
TTCGAAATTG GTCACTTCTT GCGCGCCAGA ATTATTCCAA AAGCAGTGCT TTACTACACT 
GGCGACATCG TCGACGATGA GGACGATGAG GACGAAGAGG AGTATGACGA GAACGAGGAG 
GATGAGTACG ACGACGACGA TGCACCGCCA CCAAAGGGCC CCAAATCAGC CGGTATCAAG 
AAGCAGTCGC CCAACGACTG CCCGAATCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246485.1
CDS	142..1254
Translation	MDAPAEGHVDPESCNEIEDEKSGSDCQSMPAYMNSVMRRQYLQQMVKMLPAPVQNRIVFLKNLQLQHLNIEAQFFEDVYKLEQKYQVQYQPLFDKRREIIEGKVDPAEEKPQWKEPESSTDNEADAEHFREALSSLKSIPKDAKGIPGFWLTVFRNTAIMSEMVQPHDEPAIRKLIDISIKYDNGHSYTLEFHFDKNEYFSNSVLTKQYVLKSTVDPNDPFAFEGPEIYKCTGCTINWEKKMNLTVKTIRKKQKHKERGAVRTIVKQVPTDSFFNFFSPPEVPSDQEEVDDDSQQILATDFEIGHFLRARIIPKAVLYYTGDIVDDEDDEDEEEYDENEEDEYDDDDAPPPKGPKSAGIKKQSPNDCPNQ

Target ORF information:

RefSeq Version	NM_001259556.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nucleosome assembly protein 1, transcript variant B (Nap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259556.1

ORF Insert Sequence:

ATGGACGCCC CAGCCGAAGG ACACGTTGAC CCCGAGTCCT GCAACGAAAT CGAGGACGAG 
AAGTCCGGCT CGGACTGCCA GTCGATGCCC GCCTACATGA ACTCGGTCAT GCGGCGACAG 
TATCTGCAGC AAATGGTCAA GATGCTGCCG GCTCCAGTGC AGAATCGGAT TGTGTTCCTG 
AAAAACCTAC AGCTGCAGCA CCTGAATATC GAGGCCCAGT TCTTTGAGGA CGTCTACAAG 
CTGGAGCAGA AGTACCAGGT GCAGTACCAG CCGTTGTTCG ATAAGCGCAG GGAGATCATC 
GAGGGCAAGG TGGACCCCGC CGAGGAGAAG CCCCAGTGGA AGGAACCGGA GTCGTCGACC 
GACAACGAGG CCGATGCCGA GCACTTCCGC GAGGCCCTGA GCAGTTTGAA AAGTATTCCC 
AAGGATGCCA AGGGCATTCC CGGCTTCTGG CTGACGGTGT TCCGCAACAC GGCCATAATG 
TCTGAGATGG TGCAGCCGCA CGACGAGCCC GCCATCCGCA AGCTAATCGA TATTTCCATC 
AAGTACGACA ACGGGCATTC GTACACCTTG GAGTTTCACT TCGACAAGAA CGAGTACTTC 
TCCAACTCAG TTCTCACAAA GCAGTACGTT TTGAAGTCCA CCGTGGATCC CAACGATCCG 
TTCGCATTTG AAGGCCCAGA GATCTACAAG TGCACGGGGT GCACCATTAA CTGGGAGAAG 
AAGATGAACC TTACTGTGAA GACCATCCGC AAGAAGCAGA AGCACAAGGA GCGCGGCGCA 
GTGCGCACCA TTGTGAAGCA GGTTCCGACG GATTCCTTCT TCAATTTCTT CAGCCCACCA 
GAGGTCCCGA GCGACCAGGA GGAAGTCGAC GACGACTCCC AGCAGATCCT GGCCACCGAC 
TTCGAAATTG GTCACTTCTT GCGCGCCAGA ATTATTCCAA AAGCAGTGCT TTACTACACT 
GGCGACATCG TCGACGATGA GGACGATGAG GACGAAGAGG AGTATGACGA GAACGAGGAG 
GATGAGTACG ACGACGACGA TGCACCGCCA CCAAAGGGCC CCAAATCAGC CGGTATCAAG 
AAGCAGTCGC CCAACGACTG CCCGAATCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nap1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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