Tyler cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tyler
Entrez Gene ID	3772349
Full Name	CG14208 gene product from transcript CG14208-RF
Synonyms	CG14208,CG33075,Dmel\CG14208
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 18D3-18D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_167671.2	NP_728278.2	tyler, isoform G
NM_134483.3	NP_608327.2	tyler, isoform B
NM_001298518.1	NP_001285447.1	tyler, isoform F

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tyler gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tyler cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa02601	NM_134483.3 Latest version!	Drosophila melanogaster tyler, transcript variant B (Tyler), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa22390	NM_001298518.1 Latest version!	Drosophila melanogaster tyler, transcript variant F (Tyler), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa22391	NM_167671.2 Latest version!	Drosophila melanogaster tyler, transcript variant G (Tyler), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tyler ( NM_134483.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608327.2 Drosophila melanogaster tyler, transcript variant B (Tyler), mRNA.

Price & Availability↑

CloneID	OFa02601
Clone ID Related Accession (Same CDS sequence)	NM_134483.3
Accession Version	NM_134483.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tyler, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_134483.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGCCGC AGCTAATGTC CCAGATGGAA AATGGCGGCG AGGAGGAAGG GTGTCGTTTG 
GAGTGCAAGG AAATGGATCC GCTGCGGCTA ACCACGCTGA TCCTGAGTGC GGATCCACGG 
TATCGCATCA AGCCAATGCA GCAAGTCGTG TCCGCCCTCG TTGGCGGTCT CATCACGACA 
TTTGTGGTGA CGCCCTTGGA GGTGGTGAAA ACGCGCGTCC AGACGCAGCA CGCGATCCGC 
CAGCGACCGA CTGTTTCGAA GCTCTGCTAC GTCTATCACA ACGGCCTGAT GACCCACGTG 
TGCCGCTCCA GTGATATCTG TGTCCCGAAA CCAGGCCGTG ATCCGCAAAA TCTTCGGCCG 
CTACGCGGCG CCATGGATGC CTTTGTCAAG ATCGTGTGCA CAAGTGGATT CAGTGGTCTG 
TGGGCGGGTC TAAGTCCCAC TTTAGTTTCG GCCCTGCCCT CCACGATTAT CTACTTTCTG 
ACGTACGAGT ATATTAAGAA TTCGCTATCC CACATCTACT TGGTCTCGCA GAAGTTTGAG 
GAATCCGGCA TGAAGGACCA AGTGCCTGGA GCCGACGGCG GAGATCCCCT GGACCAGGCA 
ACCAGGGGAA TAAATGTGAG CGCCACCGCA CCGGTATCCA CCGCATCGCT GCCCTACTAC 
GTGCCCATGG CCTCGGGCAT TTGCTCGCGC ACCATTGTGG TCACGGCCAT TACGCCCATC 
GAAATGGTTC GCATCAAGAT GCAGTCGGAG TACATGACCT ACGCCGAGCT GTGGCGTGTG 
CTGCGCTCGT TGATCCGCCA GCACGGCATC CTGGGTCTTT GGCGGGGCTG GCCGCCCACC 
GTGATGCGGG ACGCACCCTT CTCCGGAACC TATTGGGCAG TCTACGAGGC GATTAAGCGG 
GCCTTCAGCG TCACGGAGCC GACTTTCCTC TTTAGCTTCC TTACGGGCGC CATCTCCGGA 
GCGGTGGCTA CCTTTGTGAC CATGCCCTTT GACCTGATCA CCACGCACAC GCAGATTGAG 
CTGGGTCAGG ACGTTCTGTA CGAGGAGATT GGAGCGGGAA CAGGAGCGGG AACGGGAACA 
GGAGCAGGAG CACGACCAAA GACACCGCAA AGTGCCGTCG CCAATTCCAG GCCATCGGTG 
CTCTCGCGAA TGCGTCAAAT TTACAGGCTG CAGGGAGTGC GGGGCCTCTA TGTCGGGGTG 
ATGCCGCGCA TGCTACGTGT GGTGCCCGCC TGTGCCATCA TGATCTCCAC CTTCGAATAC 
AGCAAGTCGT TCTTCTTCCA CTACAATCTG GATCTCCAGG AGGCAGCTTA CCGGCGCTCC 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608327.2
CDS	232..1557
Translation	MPPQLMSQMENGGEEEGCRLECKEMDPLRLTTLILSADPRYRIKPMQQVVSALVGGLITTFVVTPLEVVKTRVQTQHAIRQRPTVSKLCYVYHNGLMTHVCRSSDICVPKPGRDPQNLRPLRGAMDAFVKIVCTSGFSGLWAGLSPTLVSALPSTIIYFLTYEYIKNSLSHIYLVSQKFEESGMKDQVPGADGGDPLDQATRGINVSATAPVSTASLPYYVPMASGICSRTIVVTAITPIEMVRIKMQSEYMTYAELWRVLRSLIRQHGILGLWRGWPPTVMRDAPFSGTYWAVYEAIKRAFSVTEPTFLFSFLTGAISGAVATFVTMPFDLITTHTQIELGQDVLYEEIGAGTGAGTGTGAGARPKTPQSAVANSRPSVLSRMRQIYRLQGVRGLYVGVMPRMLRVVPACAIMISTFEYSKSFFFHYNLDLQEAAYRRSQ

Target ORF information:

RefSeq Version	NM_134483.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tyler, transcript variant B (Tyler), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134483.3

ORF Insert Sequence:

ATGCCGCCGC AGCTAATGTC CCAGATGGAA AATGGCGGCG AGGAGGAAGG GTGTCGTTTG 
GAGTGCAAGG AAATGGATCC GCTGCGGCTA ACCACGCTGA TCCTGAGTGC GGATCCACGG 
TATCGCATCA AGCCAATGCA GCAAGTCGTG TCCGCCCTCG TTGGCGGTCT CATCACGACA 
TTTGTGGTGA CGCCCTTGGA GGTGGTGAAA ACGCGCGTCC AGACGCAGCA CGCGATCCGC 
CAGCGACCGA CTGTTTCGAA GCTCTGCTAC GTCTATCACA ACGGCCTGAT GACCCACGTG 
TGCCGCTCCA GTGATATCTG TGTCCCGAAA CCAGGCCGTG ATCCGCAAAA TCTTCGGCCG 
CTACGCGGCG CCATGGATGC CTTTGTCAAG ATCGTGTGCA CAAGTGGATT CAGTGGTCTG 
TGGGCGGGTC TAAGTCCCAC TTTAGTTTCG GCCCTGCCCT CCACGATTAT CTACTTTCTG 
ACGTACGAGT ATATTAAGAA TTCGCTATCC CACATCTACT TGGTCTCGCA GAAGTTTGAG 
GAATCCGGCA TGAAGGACCA AGTGCCTGGA GCCGACGGCG GAGATCCCCT GGACCAGGCA 
ACCAGGGGAA TAAATGTGAG CGCCACCGCA CCGGTATCCA CCGCATCGCT GCCCTACTAC 
GTGCCCATGG CCTCGGGCAT TTGCTCGCGC ACCATTGTGG TCACGGCCAT TACGCCCATC 
GAAATGGTTC GCATCAAGAT GCAGTCGGAG TACATGACCT ACGCCGAGCT GTGGCGTGTG 
CTGCGCTCGT TGATCCGCCA GCACGGCATC CTGGGTCTTT GGCGGGGCTG GCCGCCCACC 
GTGATGCGGG ACGCACCCTT CTCCGGAACC TATTGGGCAG TCTACGAGGC GATTAAGCGG 
GCCTTCAGCG TCACGGAGCC GACTTTCCTC TTTAGCTTCC TTACGGGCGC CATCTCCGGA 
GCGGTGGCTA CCTTTGTGAC CATGCCCTTT GACCTGATCA CCACGCACAC GCAGATTGAG 
CTGGGTCAGG ACGTTCTGTA CGAGGAGATT GGAGCGGGAA CAGGAGCGGG AACGGGAACA 
GGAGCAGGAG CACGACCAAA GACACCGCAA AGTGCCGTCG CCAATTCCAG GCCATCGGTG 
CTCTCGCGAA TGCGTCAAAT TTACAGGCTG CAGGGAGTGC GGGGCCTCTA TGTCGGGGTG 
ATGCCGCGCA TGCTACGTGT GGTGCCCGCC TGTGCCATCA TGATCTCCAC CTTCGAATAC 
AGCAAGTCGT TCTTCTTCCA CTACAATCTG GATCTCCAGG AGGCAGCTTA CCGGCGCTCC 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tyler ( NM_001298518.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285447.1 Drosophila melanogaster tyler, transcript variant F (Tyler), mRNA.

Price & Availability↑

CloneID	OFa22390
Clone ID Related Accession (Same CDS sequence)	NM_001298518.1
Accession Version	NM_001298518.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tyler, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGCCGC AGCTAATGTC CCAGATGGAA AATGGCGGCG AGGAGGAAGG GTGTCGTTTG 
GAGTGCAAGG AAATGGATCC GCTGCGGCTA ACCACGCTGA TCCTGAGTGC GGATCCACGG 
TATCGCATCA AGCCAATGCA GCAAGTCGTG TCCGCCCTCG TTGGCGGTCT CATCACGACA 
TTTGTGGTGA CGCCCTTGGA GGTGGTGAAA ACGCGCGTCC AGACGCAGCA CGCGATCCGC 
CAGCGACCGA CTGTTTCGAA GCTCTGCTAC GTCTATCACA ACGGCCTGAT GACCCACGTG 
TGCCGCTCCA GTGATATCTG TGTCCCGAAA CCAGGCCGTG ATCCGCAAAA TCTTCGGCCG 
CTACGCGGCG CCATGGATGC CTTTGTCAAG ATCGTGTGCA CAAGTGGATT CAGTGGTCTG 
TGGGCGGGTC TAAGTCCCAC TTTAGTTTCG GCCCTGCCCT CCACGATTAT CTACTTTCTG 
ACGTACGAGT ATATTAAGAA TTCGCTATCC CACATCTACT TGGTCTCGCA GAAGTTTGAG 
GAATCCGGCA TGAAGGACCA AGTGCCTGGA GCCGACGGCG GAGATCCCCT GGACCAGGCA 
ACCAGGGGAA TAAATGTGAG CGCCACCGCA CCGGTATCCA CCGCATCGCT GCCCTACTAC 
GTGCCCATGG CCTCGGGCAT TTGCTCGCGC ACCATTGTGG TCACGGCCAT TACGCCCATC 
GAAATGGTTC GCATCAAGAT GCAGTCGGAG TACATGACCT ACGCCGAGCT GTGGCGTGTG 
CTGCGCTCGT TGATCCGCCA GCACGGCATC CTGGGTCTTT GGCGGGGCTG GCCGCCCACC 
GTGATGCGGG ACGCACCCTT CTCCGGAACC TATTGGGCAG TCTACGAGGC GATTAAGCGG 
GCCTTCAGCG TCACGGAGCC GACTTTCCTC TTTAGCTTCC TTACGGGCGC CATCTCCGGA 
GCGGTGGGTG AGGGTGGCTA CCTTTGTGAC CATGCCCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285447.1
CDS	232..1233
Translation	MPPQLMSQMENGGEEEGCRLECKEMDPLRLTTLILSADPRYRIKPMQQVVSALVGGLITTFVVTPLEVVKTRVQTQHAIRQRPTVSKLCYVYHNGLMTHVCRSSDICVPKPGRDPQNLRPLRGAMDAFVKIVCTSGFSGLWAGLSPTLVSALPSTIIYFLTYEYIKNSLSHIYLVSQKFEESGMKDQVPGADGGDPLDQATRGINVSATAPVSTASLPYYVPMASGICSRTIVVTAITPIEMVRIKMQSEYMTYAELWRVLRSLIRQHGILGLWRGWPPTVMRDAPFSGTYWAVYEAIKRAFSVTEPTFLFSFLTGAISGAVGEGGYLCDHAL

Target ORF information:

RefSeq Version	NM_001298518.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tyler, transcript variant F (Tyler), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298518.1

ORF Insert Sequence:

ATGCCGCCGC AGCTAATGTC CCAGATGGAA AATGGCGGCG AGGAGGAAGG GTGTCGTTTG 
GAGTGCAAGG AAATGGATCC GCTGCGGCTA ACCACGCTGA TCCTGAGTGC GGATCCACGG 
TATCGCATCA AGCCAATGCA GCAAGTCGTG TCCGCCCTCG TTGGCGGTCT CATCACGACA 
TTTGTGGTGA CGCCCTTGGA GGTGGTGAAA ACGCGCGTCC AGACGCAGCA CGCGATCCGC 
CAGCGACCGA CTGTTTCGAA GCTCTGCTAC GTCTATCACA ACGGCCTGAT GACCCACGTG 
TGCCGCTCCA GTGATATCTG TGTCCCGAAA CCAGGCCGTG ATCCGCAAAA TCTTCGGCCG 
CTACGCGGCG CCATGGATGC CTTTGTCAAG ATCGTGTGCA CAAGTGGATT CAGTGGTCTG 
TGGGCGGGTC TAAGTCCCAC TTTAGTTTCG GCCCTGCCCT CCACGATTAT CTACTTTCTG 
ACGTACGAGT ATATTAAGAA TTCGCTATCC CACATCTACT TGGTCTCGCA GAAGTTTGAG 
GAATCCGGCA TGAAGGACCA AGTGCCTGGA GCCGACGGCG GAGATCCCCT GGACCAGGCA 
ACCAGGGGAA TAAATGTGAG CGCCACCGCA CCGGTATCCA CCGCATCGCT GCCCTACTAC 
GTGCCCATGG CCTCGGGCAT TTGCTCGCGC ACCATTGTGG TCACGGCCAT TACGCCCATC 
GAAATGGTTC GCATCAAGAT GCAGTCGGAG TACATGACCT ACGCCGAGCT GTGGCGTGTG 
CTGCGCTCGT TGATCCGCCA GCACGGCATC CTGGGTCTTT GGCGGGGCTG GCCGCCCACC 
GTGATGCGGG ACGCACCCTT CTCCGGAACC TATTGGGCAG TCTACGAGGC GATTAAGCGG 
GCCTTCAGCG TCACGGAGCC GACTTTCCTC TTTAGCTTCC TTACGGGCGC CATCTCCGGA 
GCGGTGGGTG AGGGTGGCTA CCTTTGTGAC CATGCCCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tyler ( NM_167671.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728278.2 Drosophila melanogaster tyler, transcript variant G (Tyler), mRNA.

Price & Availability↑

CloneID	OFa22391
Clone ID Related Accession (Same CDS sequence)	NM_167671.2
Accession Version	NM_167671.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tyler, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167671.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGCCGC AGCTAATGTC CCAGATGGAA AATGGCGGCG AGGAGGAAGG GTGTCGTTTG 
GAGTGCAAGG AAATGGATCC GCTGCGGCTA ACCACGCTGA TCCTGAGTGC GGATCCACGG 
TATCGCATCA AGCCAATGCA GCAAGTCGTG TCCGCCCTCG TTGGCGGTCT CATCACGACA 
TTTGTGGTGA CGCCCTTGGA GGTGGTGAAA ACGCGCGTCC AGACGCAGCA CGCGATCCGC 
CAGCGACCGA CTGTTTCGAA GCTCTGCTAC GTCTATCACA ACGGCCTGAT GACCCACGTG 
TGCCGCTCCA GTGATATCTG TGTCCCGAAA CCAGGCCGTG ATCCGCAAAA TCTTCGGCCG 
CTACGCGGCG CCATGGATGC CTTTGTCAAG ATCGTGTGCA CAAGTGGATT CAGTGGTCTG 
TGGGCGGGTC TAAGTCCCAC TTTAGTTTCG GCCCTGCCCT CCACGATTAT CTACTTTCTG 
ACGTACGAGT ATATTAAGAA TTCGCTATCC CACATCTACT TGGTCTCGCA GAAGTTTGAG 
GAATCCGGCA TGAAGGACCA AGTGCCTGGA GCCGACGGCG GAGATCCCCT GGACCAGGCA 
ACCAGGGGAA TAAATGTGAG CGCCACCGCA CCGGTATCCA CCGCATCGCT GCCCTACTAC 
GTGCCCATGG CCTCGGGCAT TTGCTCGCGC ACCATTGTGG TCACGGCCAT TACGCCCATC 
GAAATGGTTC GCATCAAGAT GCAGTCGGAG TACATGACCT ACGCCGAGCT GTGGCGTGTG 
CTGCGCTCGT TGATCCGCCA GCACGGCATC CTGGGTCTTT GGCGGGGCTG GCCGCCCACC 
GTGATGCGGG ACGCACCCTT CTCCGGAACC TATTGGGCAG TCTACGAGGC GATTAAGCGG 
GCCTTCAGCG TCACGGAGCC GACTTTCCTC TTTAGCTTCC TTACGGGCGC CATCTCCGGA 
GCGGTGGGTG AGGGTGAGCA GTGCTCTGCC ACACACCGAA AACCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728278.2
CDS	232..1239
Translation	MPPQLMSQMENGGEEEGCRLECKEMDPLRLTTLILSADPRYRIKPMQQVVSALVGGLITTFVVTPLEVVKTRVQTQHAIRQRPTVSKLCYVYHNGLMTHVCRSSDICVPKPGRDPQNLRPLRGAMDAFVKIVCTSGFSGLWAGLSPTLVSALPSTIIYFLTYEYIKNSLSHIYLVSQKFEESGMKDQVPGADGGDPLDQATRGINVSATAPVSTASLPYYVPMASGICSRTIVVTAITPIEMVRIKMQSEYMTYAELWRVLRSLIRQHGILGLWRGWPPTVMRDAPFSGTYWAVYEAIKRAFSVTEPTFLFSFLTGAISGAVGEGEQCSATHRKP

Target ORF information:

RefSeq Version	NM_167671.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tyler, transcript variant G (Tyler), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167671.2

ORF Insert Sequence:

ATGCCGCCGC AGCTAATGTC CCAGATGGAA AATGGCGGCG AGGAGGAAGG GTGTCGTTTG 
GAGTGCAAGG AAATGGATCC GCTGCGGCTA ACCACGCTGA TCCTGAGTGC GGATCCACGG 
TATCGCATCA AGCCAATGCA GCAAGTCGTG TCCGCCCTCG TTGGCGGTCT CATCACGACA 
TTTGTGGTGA CGCCCTTGGA GGTGGTGAAA ACGCGCGTCC AGACGCAGCA CGCGATCCGC 
CAGCGACCGA CTGTTTCGAA GCTCTGCTAC GTCTATCACA ACGGCCTGAT GACCCACGTG 
TGCCGCTCCA GTGATATCTG TGTCCCGAAA CCAGGCCGTG ATCCGCAAAA TCTTCGGCCG 
CTACGCGGCG CCATGGATGC CTTTGTCAAG ATCGTGTGCA CAAGTGGATT CAGTGGTCTG 
TGGGCGGGTC TAAGTCCCAC TTTAGTTTCG GCCCTGCCCT CCACGATTAT CTACTTTCTG 
ACGTACGAGT ATATTAAGAA TTCGCTATCC CACATCTACT TGGTCTCGCA GAAGTTTGAG 
GAATCCGGCA TGAAGGACCA AGTGCCTGGA GCCGACGGCG GAGATCCCCT GGACCAGGCA 
ACCAGGGGAA TAAATGTGAG CGCCACCGCA CCGGTATCCA CCGCATCGCT GCCCTACTAC 
GTGCCCATGG CCTCGGGCAT TTGCTCGCGC ACCATTGTGG TCACGGCCAT TACGCCCATC 
GAAATGGTTC GCATCAAGAT GCAGTCGGAG TACATGACCT ACGCCGAGCT GTGGCGTGTG 
CTGCGCTCGT TGATCCGCCA GCACGGCATC CTGGGTCTTT GGCGGGGCTG GCCGCCCACC 
GTGATGCGGG ACGCACCCTT CTCCGGAACC TATTGGGCAG TCTACGAGGC GATTAAGCGG 
GCCTTCAGCG TCACGGAGCC GACTTTCCTC TTTAGCTTCC TTACGGGCGC CATCTCCGGA 
GCGGTGGGTG AGGGTGAGCA GTGCTCTGCC ACACACCGAA AACCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Tyler cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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