sotv cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	sotv
Entrez Gene ID	3772101
Full Name	CG8433 gene product from transcript CG8433-RB
Synonyms	BcDNA:GH02288,CG33038,CG8433,DEXT2,DExt2,Dmel\CG8433,Sotv,dExt2,sotv
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 52E11-52E11

mRNA and Protein(s)

mRNA	Protein	Name
NM_166150.3	NP_725536.1	sister of tout-velu

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Ext2	NP_725536.1
Canis lupus familiaris (dog)	EXT2	XP_005631368.1
Rattus norvegicus (Norway rat)	Ext2	NP_001101221.1
Danio rerio (zebrafish)	ext2	NP_001008400.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000081	XP_311070.5
Homo sapiens (human)	EXT2	NP_001171554.1
Pan troglodytes (chimpanzee)	EXT2	XP_003313041.1
	EXT2	XP_001106468.2
Gallus gallus (chicken)	EXT2	NP_001026520.1
Xenopus tropicalis (tropical clawed frog)	ext2	NP_001120887.1
Bos taurus (cattle)	EXT2	NP_803462.2
Mus musculus (house mouse)	Ext2	NP_034293.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results suggest that all three Drosophila EXT proteins -- tout-velu, brother of tout-velu, and sister of tout-velu -- are required for the biosynthesis of heparan sulfate proteoglycans, and for the gradient formation of morphogens.
Title: Distinct and collaborative roles of Drosophila EXT family proteins in morphogen signalling and gradient formation.

The following sotv gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sotv cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11847	NM_166150.3 Latest version!	Drosophila melanogaster sister of tout-velu (sotv), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sotv ( NM_166150.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725536.1 Drosophila melanogaster sister of tout-velu (sotv), mRNA.

Price & Availability↑

CloneID	OFa11847
Clone ID Related Accession (Same CDS sequence)	NM_166150.3
Accession Version	NM_166150.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sister of tout-velu
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166150.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAAAAA TAAAGAATCT GCTGAGTTTT GTGACCCAGT CACGGGCCAT ATCGCACACC 
AATCCCAGGG AACATATACT TAACTGCCTG ACCTATGGGC TACTAGTCAT TGTGGCGCTC 
TGCGCGGGTT TTCTGCTTTG GGATCTCTCC AGCAGCCCAC GGGATGGATT CTTCCATGGG 
AAAAGGGACT CGCACACATT AATCCTCGAC CTGGAGCACA TCCAGGAGCT TGCCGTAAAT 
CCGGAGGCCG AACAGCGGGC ACGGAATGTA AACTGCACCT TCTGGGACTG CCTGAACATC 
TACAAATGCG AGCACGACCG CCTAAAGGTC TACATCTATC CGCTGCAGGA GTTTGTTGAC 
GAGCAGAGCG ACAAAACGGC CACCACCCTG TCCAGCGAGT ACTTCCAAAT ATTGGAGGCG 
GTACTAAAGA GTCGCTACTA CACGTCCAAT CCGAACGAAG CGTGCCTCTT TCTGCCCAGT 
TTGGATCTCC TGAACCAAAA TGTCTTCGAC AAGCACTTGG CGGGCGCTGC CTTGGCTTCA 
CTGGACTTCT GGGATCGTGG TGCCAACCAC ATCATCTTTA ATATGCTGCC CGGCGGAGCT 
CCATCCTATA ACACTGTGCT GGATGTGAAT ACGGATAATG CCATCATTTT TGGCGGTGGC 
TTTGACTCTT GGTCTTATCG CCCGGGCTTT GATGTCGCCA TTCCAGTCTG GAGTCCACGA 
TTGGTGCGAC AACATGCACA TGCGACGGCC CAGAGGAAGT TCCTATTGGT AGTAGCCCAG 
CTGAACATTC TTCCGCGCTT TGTGAGGACT CTGAGGGAGT TATCTCTGGC TCACAGCGAG 
CAATTGCTTC TTTTGGGAGC CTGCGAGAAC TTGGATCTCA CAATGCGCTG CCCTTTGTCG 
CAGCACCACA AATCGCTGGA GTATCCTCGG CTGCTGTCTC GCGGTAAATT TTGCCTGCTA 
GGTCGTAGTC TTCGCATGGG TCAACCCGAT CTAGTAGAGA TTATGTCTCA GCACTGTATT 
CCCGTAATTG CCGTGGACAA CTATGTGCTA CCCTTCGAAG ATGTGATTGA TTGGTCATTG 
GCCTCCGTTC GTATACGCGA AAACGAACTG CATTCCGTAA TGCAGAAGCT AAAAGCAATA 
TCGAGTGTTA AGATAGTTGA AATGCAGAAG CAAGTGCAGT GGTTGTTCTC CAAGTACTTC 
AAAGATCTGA AGACTGTGAC ACTAACTGCT CTTGAGGTGC TGGAGAGTAG GATCTTTCCG 
CTGCGAGCCC GTAGCAGTCG TCAGTGGAAT ACCATTGATA CCAACGCCCG TTCCACATTC 
AATCCCCTTT TCCTGCCGTC GCTGGCACCA AAGTCACAGG GCTTCACCGC CGTCATCCTC 
ACCTACGATC GCGTGGAGAG TCTGTTCCTG CTGATTCAGA AGCTCGCTGT AGTGCCATCA 
TTGCAAAGCA TTTTAGTGAT TTGGAATAAT CAGAAAAAAT CTCCACCGCA CTTATCCACC 
TTTCCATCTA TATCGAAACC GCTGAAAATT CGGCAAACCA AGGAGAACAA GCTATCCAAT 
CGATTCTATC CATATCCAGA AATAGAAACG GAAGCTATCT TAACCATAGA CGATGACATC 
ATAATGCTGA CCACTGATGA GTTGGATTTT GGCTATGAAG TTTGGCGAGA GTTTCCTGAT 
CACATTGTTG GCTTTCCCAG TCGAATTCAT GTTTGGGAAA ATGTTACGAT GCGCTGGCAC 
TACGAATCGG AGTGGACGAA CCAAATATCA ATGGTTCTTA CCGGCGCTGC TTTCCATCAC 
AAGTACTGGA GTCACATGTA CACACATGCC ATGCCGGGTG ATATCAAGGA TTGGGTGGAT 
GAGCACATGA ATTGCGAGGA CATTGCCATG AATTTTCTGG TGGCCAACAT CACCAACAAT 
CCGCCCATTA AGGTTACTCC TCGAAAGAAG TTTAAGTGCC CGGAGTGCAC CAATACGGAA 
ATGCTCTCCG CTGATCTGAA TCACATGCGT GAACGTTCGG CTTGCATTGA TCGCTTCTCC 
AAAATCTACG GCCGCATGCC ACTTCGCACG GTGGAGTTCA GAGCGGACCC TGTGTTATTC 
CGGGACAATT TCCCGGACAA GTTGAAGCGT TACAATGATA TTGGTAGCTT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725536.1
CDS	63..2216
Translation	MTKIKNLLSFVTQSRAISHTNPREHILNCLTYGLLVIVALCAGFLLWDLSSSPRDGFFHGKRDSHTLILDLEHIQELAVNPEAEQRARNVNCTFWDCLNIYKCEHDRLKVYIYPLQEFVDEQSDKTATTLSSEYFQILEAVLKSRYYTSNPNEACLFLPSLDLLNQNVFDKHLAGAALASLDFWDRGANHIIFNMLPGGAPSYNTVLDVNTDNAIIFGGGFDSWSYRPGFDVAIPVWSPRLVRQHAHATAQRKFLLVVAQLNILPRFVRTLRELSLAHSEQLLLLGACENLDLTMRCPLSQHHKSLEYPRLLSRGKFCLLGRSLRMGQPDLVEIMSQHCIPVIAVDNYVLPFEDVIDWSLASVRIRENELHSVMQKLKAISSVKIVEMQKQVQWLFSKYFKDLKTVTLTALEVLESRIFPLRARSSRQWNTIDTNARSTFNPLFLPSLAPKSQGFTAVILTYDRVESLFLLIQKLAVVPSLQSILVIWNNQKKSPPHLSTFPSISKPLKIRQTKENKLSNRFYPYPEIETEAILTIDDDIIMLTTDELDFGYEVWREFPDHIVGFPSRIHVWENVTMRWHYESEWTNQISMVLTGAAFHHKYWSHMYTHAMPGDIKDWVDEHMNCEDIAMNFLVANITNNPPIKVTPRKKFKCPECTNTEMLSADLNHMRERSACIDRFSKIYGRMPLRTVEFRADPVLFRDNFPDKLKRYNDIGSL

Target ORF information:

RefSeq Version	NM_166150.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sister of tout-velu (sotv), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166150.3

ORF Insert Sequence:

ATGACAAAAA TAAAGAATCT GCTGAGTTTT GTGACCCAGT CACGGGCCAT ATCGCACACC 
AATCCCAGGG AACATATACT TAACTGCCTG ACCTATGGGC TACTAGTCAT TGTGGCGCTC 
TGCGCGGGTT TTCTGCTTTG GGATCTCTCC AGCAGCCCAC GGGATGGATT CTTCCATGGG 
AAAAGGGACT CGCACACATT AATCCTCGAC CTGGAGCACA TCCAGGAGCT TGCCGTAAAT 
CCGGAGGCCG AACAGCGGGC ACGGAATGTA AACTGCACCT TCTGGGACTG CCTGAACATC 
TACAAATGCG AGCACGACCG CCTAAAGGTC TACATCTATC CGCTGCAGGA GTTTGTTGAC 
GAGCAGAGCG ACAAAACGGC CACCACCCTG TCCAGCGAGT ACTTCCAAAT ATTGGAGGCG 
GTACTAAAGA GTCGCTACTA CACGTCCAAT CCGAACGAAG CGTGCCTCTT TCTGCCCAGT 
TTGGATCTCC TGAACCAAAA TGTCTTCGAC AAGCACTTGG CGGGCGCTGC CTTGGCTTCA 
CTGGACTTCT GGGATCGTGG TGCCAACCAC ATCATCTTTA ATATGCTGCC CGGCGGAGCT 
CCATCCTATA ACACTGTGCT GGATGTGAAT ACGGATAATG CCATCATTTT TGGCGGTGGC 
TTTGACTCTT GGTCTTATCG CCCGGGCTTT GATGTCGCCA TTCCAGTCTG GAGTCCACGA 
TTGGTGCGAC AACATGCACA TGCGACGGCC CAGAGGAAGT TCCTATTGGT AGTAGCCCAG 
CTGAACATTC TTCCGCGCTT TGTGAGGACT CTGAGGGAGT TATCTCTGGC TCACAGCGAG 
CAATTGCTTC TTTTGGGAGC CTGCGAGAAC TTGGATCTCA CAATGCGCTG CCCTTTGTCG 
CAGCACCACA AATCGCTGGA GTATCCTCGG CTGCTGTCTC GCGGTAAATT TTGCCTGCTA 
GGTCGTAGTC TTCGCATGGG TCAACCCGAT CTAGTAGAGA TTATGTCTCA GCACTGTATT 
CCCGTAATTG CCGTGGACAA CTATGTGCTA CCCTTCGAAG ATGTGATTGA TTGGTCATTG 
GCCTCCGTTC GTATACGCGA AAACGAACTG CATTCCGTAA TGCAGAAGCT AAAAGCAATA 
TCGAGTGTTA AGATAGTTGA AATGCAGAAG CAAGTGCAGT GGTTGTTCTC CAAGTACTTC 
AAAGATCTGA AGACTGTGAC ACTAACTGCT CTTGAGGTGC TGGAGAGTAG GATCTTTCCG 
CTGCGAGCCC GTAGCAGTCG TCAGTGGAAT ACCATTGATA CCAACGCCCG TTCCACATTC 
AATCCCCTTT TCCTGCCGTC GCTGGCACCA AAGTCACAGG GCTTCACCGC CGTCATCCTC 
ACCTACGATC GCGTGGAGAG TCTGTTCCTG CTGATTCAGA AGCTCGCTGT AGTGCCATCA 
TTGCAAAGCA TTTTAGTGAT TTGGAATAAT CAGAAAAAAT CTCCACCGCA CTTATCCACC 
TTTCCATCTA TATCGAAACC GCTGAAAATT CGGCAAACCA AGGAGAACAA GCTATCCAAT 
CGATTCTATC CATATCCAGA AATAGAAACG GAAGCTATCT TAACCATAGA CGATGACATC 
ATAATGCTGA CCACTGATGA GTTGGATTTT GGCTATGAAG TTTGGCGAGA GTTTCCTGAT 
CACATTGTTG GCTTTCCCAG TCGAATTCAT GTTTGGGAAA ATGTTACGAT GCGCTGGCAC 
TACGAATCGG AGTGGACGAA CCAAATATCA ATGGTTCTTA CCGGCGCTGC TTTCCATCAC 
AAGTACTGGA GTCACATGTA CACACATGCC ATGCCGGGTG ATATCAAGGA TTGGGTGGAT 
GAGCACATGA ATTGCGAGGA CATTGCCATG AATTTTCTGG TGGCCAACAT CACCAACAAT 
CCGCCCATTA AGGTTACTCC TCGAAAGAAG TTTAAGTGCC CGGAGTGCAC CAATACGGAA 
ATGCTCTCCG CTGATCTGAA TCACATGCGT GAACGTTCGG CTTGCATTGA TCGCTTCTCC 
AAAATCTACG GCCGCATGCC ACTTCGCACG GTGGAGTTCA GAGCGGACCC TGTGTTATTC 
CGGGACAATT TCCCGGACAA GTTGAAGCGT TACAATGATA TTGGTAGCTT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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sotv cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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