Klp59D cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Klp59D
Entrez Gene ID	37674
Full Name	Kinesin-like protein at 59D
Synonyms	CG12192,DmKlp59D,Dmel\CG12192,KIF2A/B/C,KLP59D
General protein information	Preferred Names Kinesin-like protein at 59D Names CG12192 gene product from transcript CG12192-RA CG12192-PA Kinesin13A Klp59D-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 59D1-59D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_137918.3	NP_611762.1	Kinesin-like protein at 59D

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Klp59C	NP_611759.1
Drosophila melanogaster (fruit fly)	Klp59D	NP_611762.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results identify KLP59D as a novel and important regulator of spindle microtubule dynamics and chromosome motility, via Pacman and Flux, in Drosophila somatic cells.
Title: The Drosophila kinesin-13, KLP59D, impacts Pacman- and Flux-based chromosome movement.

The following Klp59D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Klp59D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10999	NM_137918.3 Latest version!	Drosophila melanogaster Kinesin-like protein at 59D (Klp59D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Klp59D ( NM_137918.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611762.1 Drosophila melanogaster Kinesin-like protein at 59D (Klp59D), mRNA.

Price & Availability↑

CloneID	OFa10999
Clone ID Related Accession (Same CDS sequence)	NM_137918.3
Accession Version	NM_137918.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Kinesin-like protein at 59D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137918.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCGCA TCAAAATTGG CGAGCAGCTC CTCTTCCAGA GGAGTGACGG GCGTGTCCAT 
CAGGTGGTCT GCACTGCCAA GAACCTGGAA CGGGATTCCA TCACGGGCGA ATGGACCGAG 
GGCACGGTGG TGAAGGGTAA GGAAGTGCCA TTGAGCACGC TGATTGGCAT CAACCATCAT 
ATATTCGCGG AGAATCAACC GCCGATATCG AAGCCACCGA GCTCCTTGCT GCCGAAGCCT 
CGAGGCTCGA GTCCGACCGG AGGACCTAAG ACCCACACTG GAAATCCGTA TGCAGAGCGC 
ATGAGAGTAC AGGACGGTCG CAGTAAAATT GCCACCAGAA AGACGGATCC TGCAGCGGTC 
GGAGCGGGAA ATCATGAGCT GGGTAGAGCG CACACCCCTC GTGTGCCAGC CGGTAGGCCG 
GACCGGGATG CCAGCCCCAC CCAAGGTCGC AAGTCACAGG GTGGCAACAA TAGCAACCAA 
CGCTTTTCCA GTGTAGTGAG GGAGGTGAAT CGCATGAAGG AGCAGAGGGA GAAGCGAAGG 
GCTCGCCAGG CGGAACAGCT CCAGGAGAAG GATGCACTGC GTCGCAATAA TCCGGGGAAT 
CCCAACTGGG AGGTGTCGGT GATGCTGCGC CAATACCGCT CCACCTTGAT CTTTTCTCCA 
CTTCGATGCC TGGATCCCAA TGGAGGCACT GTTCAGCAAA TTACGGTGTG TGTGCGAAAA 
CGACCCATGA GTCGCAAGGA GGAGAACTCC AAGAACCTGG ACATCATCAC AGTTCCCAGT 
GCCGACAGCC TGATCGTCCA TGAGTTGCGC CTCAAGGTGG ATCTCACCAA GTTCCTGGAG 
CACCACAAAT TCCGTTTCGA CTACACGTTC GACGAGGAGT GCTCCAATGC GCTGGTCTAC 
GATCACACTG CTCGTCCGTT GATCAGAACC ATGTTCGAGG GCGGCAATGC CACTTGTTTC 
GCTTACGGAC AAACTGGCAG CGGAAAAACG CACACCATGG GCGGAGAATT CTTCGGAAAG 
GTTCAGGATT GCGGTACCGG GATCTACGCC ATGGCAGCTC GCGATGTCTT CGAGGAGGTA 
TCGCGCCCGG AGTACCGGCA AATGGGTGCC AAGATTACGT GCAGCTTCTT CGAAATCTAT 
GGCACCAAGG TGTTCGATCT CTTGCTACCC AACAAGCCCA TGCTGCGGGT CCTAGAGGAT 
GCCAGGCAGC AGGTCGTGGT GGTGGGCCTA ACGGAGATGC CGGTGACCAA AGTGGAGGAT 
GTCCTGAGAC TGATTGAGCA CGGCAGCAAA GAGCGCACTT CCGGCCAAAC ATCGGCGAAC 
GCCAAGTCAT CGCGTTCCCA CGCCGTCTTT CAAATAGCAC TCCACTTTCC CGATTCCTGG 
GGCCCACACG GCAAGTGCTC CTTTGTGGAC TTGGCGGGCA ATGAACGCGG GGCGGATACG 
CAATCCGCCG ATCGTCAAAC TCGCATCGAG GGAGCCGAGA TCAATAAATC TCTGCTGGCC 
CTCAAGGAGT GCATTCGAGC CCTCAGCCGG CAGTCGAGTC ACCTTCCCTT CCGTGGCTCC 
AAGTTGACCC AAGTGCTGCG CGACTCCTTT GTCGGCGGCA AGAAGAACAA GACCTGCATG 
ATTGCCATGA TATCGCCATC CATGAGCTGC GTGGAGAATA CGCTCAACAC TCTACGTTAC 
GCAGACAGGG TTAAGGAGCT CATAGCCAAG GAAGACGAAC ACTTGCAGTC CGTGGAAGGG 
GATGGAGAGA AGTCTCCCGA TCTCAACGAG GAATCAGAGC CAGAAATGAT GGCCGACGAG 
GAGGGCGATG AGGAGCCGGA GGATGAGGAC AATCAGCATC TAACCATATC CTCGGAGGAG 
GCAAGCTCCT ACAACAACAT GAGTTATGAC ATGAGCTTTA ACCACACCCT CAACATTCTG 
GGTCCCTCAA GGAACGTGGA TATCCAAGAG GTGGCCGAGC AGCACGCACT TTTGGTAGAG 
AATCTAGAGA CTTACGCTCA CAATTTCCGG CAACTGAAAA CGGATAAGGA AATAGAGCAG 
TACACGCAAA ATTCAGAAAG CGCATTGATG AAACTGCTAG CAATGGTGAA TAGAACGCGG 
GATGTAACGC ACAATTACAA CACTCAGAAA TTATTGAAGG AAGAAAACCA AAATGCATAT 
AAGAAGGGCG AGCTGGATGA ATCCGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611762.1
CDS	168..2357
Translation	MDRIKIGEQLLFQRSDGRVHQVVCTAKNLERDSITGEWTEGTVVKGKEVPLSTLIGINHHIFAENQPPISKPPSSLLPKPRGSSPTGGPKTHTGNPYAERMRVQDGRSKIATRKTDPAAVGAGNHELGRAHTPRVPAGRPDRDASPTQGRKSQGGNNSNQRFSSVVREVNRMKEQREKRRARQAEQLQEKDALRRNNPGNPNWEVSVMLRQYRSTLIFSPLRCLDPNGGTVQQITVCVRKRPMSRKEENSKNLDIITVPSADSLIVHELRLKVDLTKFLEHHKFRFDYTFDEECSNALVYDHTARPLIRTMFEGGNATCFAYGQTGSGKTHTMGGEFFGKVQDCGTGIYAMAARDVFEEVSRPEYRQMGAKITCSFFEIYGTKVFDLLLPNKPMLRVLEDARQQVVVVGLTEMPVTKVEDVLRLIEHGSKERTSGQTSANAKSSRSHAVFQIALHFPDSWGPHGKCSFVDLAGNERGADTQSADRQTRIEGAEINKSLLALKECIRALSRQSSHLPFRGSKLTQVLRDSFVGGKKNKTCMIAMISPSMSCVENTLNTLRYADRVKELIAKEDEHLQSVEGDGEKSPDLNEESEPEMMADEEGDEEPEDEDNQHLTISSEEASSYNNMSYDMSFNHTLNILGPSRNVDIQEVAEQHALLVENLETYAHNFRQLKTDKEIEQYTQNSESALMKLLAMVNRTRDVTHNYNTQKLLKEENQNAYKKGELDESE

Target ORF information:

RefSeq Version	NM_137918.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Kinesin-like protein at 59D (Klp59D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137918.3

ORF Insert Sequence:

ATGGATCGCA TCAAAATTGG CGAGCAGCTC CTCTTCCAGA GGAGTGACGG GCGTGTCCAT 
CAGGTGGTCT GCACTGCCAA GAACCTGGAA CGGGATTCCA TCACGGGCGA ATGGACCGAG 
GGCACGGTGG TGAAGGGTAA GGAAGTGCCA TTGAGCACGC TGATTGGCAT CAACCATCAT 
ATATTCGCGG AGAATCAACC GCCGATATCG AAGCCACCGA GCTCCTTGCT GCCGAAGCCT 
CGAGGCTCGA GTCCGACCGG AGGACCTAAG ACCCACACTG GAAATCCGTA TGCAGAGCGC 
ATGAGAGTAC AGGACGGTCG CAGTAAAATT GCCACCAGAA AGACGGATCC TGCAGCGGTC 
GGAGCGGGAA ATCATGAGCT GGGTAGAGCG CACACCCCTC GTGTGCCAGC CGGTAGGCCG 
GACCGGGATG CCAGCCCCAC CCAAGGTCGC AAGTCACAGG GTGGCAACAA TAGCAACCAA 
CGCTTTTCCA GTGTAGTGAG GGAGGTGAAT CGCATGAAGG AGCAGAGGGA GAAGCGAAGG 
GCTCGCCAGG CGGAACAGCT CCAGGAGAAG GATGCACTGC GTCGCAATAA TCCGGGGAAT 
CCCAACTGGG AGGTGTCGGT GATGCTGCGC CAATACCGCT CCACCTTGAT CTTTTCTCCA 
CTTCGATGCC TGGATCCCAA TGGAGGCACT GTTCAGCAAA TTACGGTGTG TGTGCGAAAA 
CGACCCATGA GTCGCAAGGA GGAGAACTCC AAGAACCTGG ACATCATCAC AGTTCCCAGT 
GCCGACAGCC TGATCGTCCA TGAGTTGCGC CTCAAGGTGG ATCTCACCAA GTTCCTGGAG 
CACCACAAAT TCCGTTTCGA CTACACGTTC GACGAGGAGT GCTCCAATGC GCTGGTCTAC 
GATCACACTG CTCGTCCGTT GATCAGAACC ATGTTCGAGG GCGGCAATGC CACTTGTTTC 
GCTTACGGAC AAACTGGCAG CGGAAAAACG CACACCATGG GCGGAGAATT CTTCGGAAAG 
GTTCAGGATT GCGGTACCGG GATCTACGCC ATGGCAGCTC GCGATGTCTT CGAGGAGGTA 
TCGCGCCCGG AGTACCGGCA AATGGGTGCC AAGATTACGT GCAGCTTCTT CGAAATCTAT 
GGCACCAAGG TGTTCGATCT CTTGCTACCC AACAAGCCCA TGCTGCGGGT CCTAGAGGAT 
GCCAGGCAGC AGGTCGTGGT GGTGGGCCTA ACGGAGATGC CGGTGACCAA AGTGGAGGAT 
GTCCTGAGAC TGATTGAGCA CGGCAGCAAA GAGCGCACTT CCGGCCAAAC ATCGGCGAAC 
GCCAAGTCAT CGCGTTCCCA CGCCGTCTTT CAAATAGCAC TCCACTTTCC CGATTCCTGG 
GGCCCACACG GCAAGTGCTC CTTTGTGGAC TTGGCGGGCA ATGAACGCGG GGCGGATACG 
CAATCCGCCG ATCGTCAAAC TCGCATCGAG GGAGCCGAGA TCAATAAATC TCTGCTGGCC 
CTCAAGGAGT GCATTCGAGC CCTCAGCCGG CAGTCGAGTC ACCTTCCCTT CCGTGGCTCC 
AAGTTGACCC AAGTGCTGCG CGACTCCTTT GTCGGCGGCA AGAAGAACAA GACCTGCATG 
ATTGCCATGA TATCGCCATC CATGAGCTGC GTGGAGAATA CGCTCAACAC TCTACGTTAC 
GCAGACAGGG TTAAGGAGCT CATAGCCAAG GAAGACGAAC ACTTGCAGTC CGTGGAAGGG 
GATGGAGAGA AGTCTCCCGA TCTCAACGAG GAATCAGAGC CAGAAATGAT GGCCGACGAG 
GAGGGCGATG AGGAGCCGGA GGATGAGGAC AATCAGCATC TAACCATATC CTCGGAGGAG 
GCAAGCTCCT ACAACAACAT GAGTTATGAC ATGAGCTTTA ACCACACCCT CAACATTCTG 
GGTCCCTCAA GGAACGTGGA TATCCAAGAG GTGGCCGAGC AGCACGCACT TTTGGTAGAG 
AATCTAGAGA CTTACGCTCA CAATTTCCGG CAACTGAAAA CGGATAAGGA AATAGAGCAG 
TACACGCAAA ATTCAGAAAG CGCATTGATG AAACTGCTAG CAATGGTGAA TAGAACGCGG 
GATGTAACGC ACAATTACAA CACTCAGAAA TTATTGAAGG AAGAAAACCA AAATGCATAT 
AAGAAGGGCG AGCTGGATGA ATCCGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Klp59D cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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