YME1L cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	YME1L
Entrez Gene ID	37636
Full Name	CG3499 gene product from transcript CG3499-RD
Synonyms	DmCG3499,Dmel\CG3499
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 59B4-59B4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001259544.2	NP_001246473.1	YME1 like ATPase, isoform C
NM_166568.3	NP_726263.1	YME1 like ATPase, isoform B
NM_001274213.2	NP_001261142.1	YME1 like ATPase, isoform D

Pathways from BioSystems

Homologies

	LOC714687	XP_002805640.1
Canis lupus familiaris (dog)	YME1L1	XP_535172.3
Rattus norvegicus (Norway rat)	Yme1l1	NP_446134.2
Homo sapiens (human)	YME1L1	NP_647473.1
Danio rerio (zebrafish)	yme1l1b	NP_001082983.1
Caenorhabditis elegans (roundworm)	ymel-1	NP_499298.2
Bos taurus (cattle)	YME1L1	NP_001095320.1
Rattus norvegicus (Norway rat)	LOC100910779	XP_003751780.1
Drosophila melanogaster (fruit fly)	CG3499	NP_001261142.1
Xenopus tropicalis (tropical clawed frog)	yme1l1	XP_002933186.2
Pan troglodytes (chimpanzee)	YME1L1	XP_507710.3
Mus musculus (house mouse)	Yme1l1	NP_038799.1
Gallus gallus (chicken)	YME1L1	NP_001026683.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009973	XP_319111.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CG3499 encodes the Drosophila homolog of the human i-AAA protease YME1L.
Title: Loss of Drosophila i-AAA protease, dYME1L, causes abnormal mitochondria and apoptotic degeneration.

The following YME1L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the YME1L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa10975	NM_001274213.2 Latest version!	Drosophila melanogaster YME1 like ATPase (YME1L), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa10976	NM_001259544.2 Latest version!	Drosophila melanogaster YME1 like ATPase (YME1L), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa12603	NM_166568.3 Latest version!	Drosophila melanogaster YME1 like ATPase (YME1L), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

YME1L ( NM_001274213.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261142.1 Drosophila melanogaster YME1 like ATPase (YME1L), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa10975
Clone ID Related Accession (Same CDS sequence)	NM_001274213.2
Accession Version	NM_001274213.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	YME1 like ATPase, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001274213.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTTCCA CCACCACACA CTCCGTGCCC TACCTGTACC TAGGCAACTT CAGCAGAAAG 
CCGCACTACT ACTCCGTTAA TCGAACCAAG CTGCACGGGA GCGCGGGAGC TGCCCGGTTG 
TCCAAGTCCA CGTCCACGTC CAGCCGGAGT CATGACCTAG TGCTCGACCT GCGGAACCTG 
CTGAGCCGTT CGAGCGCCAG TATACAGGGC ATGGTGGAGA GGGCGGCTCG ACTGAATGGC 
ATTCTCGACC GGCGGCTGGT CGATGATGTG CTGGCCAAGG TGACGAGCAT GCTGCCCTCC 
ATGCGCGATG TCCGAGTCAC GTTGGAGGAG TCGGCCACAC AGATCGGTCG TGTCCAGCTG 
CAAAATTATC AGTTCGAAGT TTCGCTAACC GGTGCAGCAG GTTCCGTGCC CACAGGCGCC 
AATGTCAAGG TGATTCCAAC TATTACACCA GGTCTTCTTC GTCCGCTCTT CTCACAACAA 
CAGCTAAATC AGATACGTGG CTTTAAGACG GATCGTTCCA TTGAGGCGGA ACAAAAGCGC 
AATCCCACAA TGACCTCCCG ACTAAAGAAT GCTCTGGCCA ACTCGCCCCA GCGTCTGGAT 
GGGGACACAC CCCTTCAGGC TGAAAAACTG AGACGGCTGC TGGCCAAATC CGAAGAGCAT 
GGATTTAATA AAGCCGAGAG CCTGAAAATT GCCTTTGCCG AGGGCTACTT GGCCGCCGCT 
AACTCGGAGG ACTCTCCAAA ATCAGGAAAA ACTATGAAAT ATCTGAAGAC TTTGCAGACT 
ATCGTCGTTA TCGTCGTGTT CTTGGGCATT TTCCTGAGCT TCTTTACAAC CTCCAATGGA 
TCCGTATTTC GCATACAACT GGGCAACCAG GTTGAAGTGG ACCCTGAGGA GATCAACGTC 
ACTTTTGAGG ATGTCAAGGG TTGCGACGAA GCCAAGCAGG AGCTCAAGGA GGTCGTTGAA 
TTCCTGAAGA GTCCGGAAAA GTTCTCAAAT TTGGGCGGAA AGCTTCCAAA GGGAGTGCTC 
CTGGTTGGCC CGCCGGGTAC TGGCAAAACA CTGCTGGCCC GTGCCGTCGC CGGTGAAGCC 
AAAGTTCCTT TCTTCCATGC TGCCGGACCG GAGTTCGACG AGGTGCTCGT AGGCCAAGGA 
GCCAGACGAG TGCGTGATCT GTTCAAGGCA GCAAAGGCCC GTGCCCCTTG TGTTATCTTC 
ATTGACGAAA TCGACTCGGT GGGCGCCAAG CGAACCAACT CTGTGCTGCA TCCTTACGCC 
AATCAGACCA TCAATCAGCT GCTCTCCGAG ATGGATGGCT TCCATCAGAA CGCTGGCGTC 
ATTGTTTTGG GAGCCACCAA TCGTCGCGAC GATTTGGATC AGGCTCTGCT GCGTCCCGGC 
CGATTTGATG TAGAAGTCAT GGTGTCGACG CCCGATTTCA CTGGACGTAA GGAGATATTG 
TCTCTGTATC TGACGAAGAT TTTGCACGAT GAAATAGACT TGGACATGCT GGCGCGCGGC 
ACCTCTGGTT TTACTGGAGC GGATCTGGAA AACATGATTA ACCAAGCGGC GTTAAGAGCT 
GCCATCGATG GAGCCGAAAC GGTCAGCATG AAGCATTTGG AAACGGCACG AGACAAAGTA 
CTTATGGGAC CCGAGCGCAA GGCTCGTCTA CCGGACGAGG AGGCCAACAC CATAACCGCT 
TACCACGAGG GCGGCCACGC GATTGTGGCC TTCTACACCA AGGAGTCGCA TCCGCTGCAC 
AAGGTGACCA TCATGCCCCG CGGACCCTCC CTTGGACACA CCGCCTACAT ACCGGAGAAG 
GAGCGTTACC ATGTCACAAA GGCACAGCTG CTGGCCATGA TGGACACCAT GATGGGCGGT 
CGTGCTGCTG AGGAACTGGT CTTCGGCACG GACAAGATTA CGTCGGGAGC CAGCAGTGAT 
CTCAAGCAGG CAACCTCGAT CGCGACGCAT ATGGTCAGGG ACTGGGGCAT GTCGGACAAG 
GTCGGTCTTC GCACCATCGA GGCGTCAAAG GGTCTGGGCA CCGGTGACAC CCTTGGTCCC 
AACACCATTG AAGCCGTGGA CGCGGAGATC AAGCGCATCC TCAGCGACAG CTACGAGCGG 
GCGAAGGCTA TCCTGCGAAA ACACACCAGG GAGCACAAAG CGCTTGCGGA GGCGCTTCTC 
AAGTACGAGA CTCTTGACGC CGACGACATC AAGGCAATAC TGAATGAAAG CCAGACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261142.1
CDS	306..2525
Translation	MFSTTTHSVPYLYLGNFSRKPHYYSVNRTKLHGSAGAARLSKSTSTSSRSHDLVLDLRNLLSRSSASIQGMVERAARLNGILDRRLVDDVLAKVTSMLPSMRDVRVTLEESATQIGRVQLQNYQFEVSLTGAAGSVPTGANVKVIPTITPGLLRPLFSQQQLNQIRGFKTDRSIEAEQKRNPTMTSRLKNALANSPQRLDGDTPLQAEKLRRLLAKSEEHGFNKAESLKIAFAEGYLAAANSEDSPKSGKTMKYLKTLQTIVVIVVFLGIFLSFFTTSNGSVFRIQLGNQVEVDPEEINVTFEDVKGCDEAKQELKEVVEFLKSPEKFSNLGGKLPKGVLLVGPPGTGKTLLARAVAGEAKVPFFHAAGPEFDEVLVGQGARRVRDLFKAAKARAPCVIFIDEIDSVGAKRTNSVLHPYANQTINQLLSEMDGFHQNAGVIVLGATNRRDDLDQALLRPGRFDVEVMVSTPDFTGRKEILSLYLTKILHDEIDLDMLARGTSGFTGADLENMINQAALRAAIDGAETVSMKHLETARDKVLMGPERKARLPDEEANTITAYHEGGHAIVAFYTKESHPLHKVTIMPRGPSLGHTAYIPEKERYHVTKAQLLAMMDTMMGGRAAEELVFGTDKITSGASSDLKQATSIATHMVRDWGMSDKVGLRTIEASKGLGTGDTLGPNTIEAVDAEIKRILSDSYERAKAILRKHTREHKALAEALLKYETLDADDIKAILNESQT

Target ORF information:

RefSeq Version	NM_001274213.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster YME1 like ATPase (YME1L), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274213.2

ORF Insert Sequence:

ATGTTTTCCA CCACCACACA CTCCGTGCCC TACCTGTACC TAGGCAACTT CAGCAGAAAG 
CCGCACTACT ACTCCGTTAA TCGAACCAAG CTGCACGGGA GCGCGGGAGC TGCCCGGTTG 
TCCAAGTCCA CGTCCACGTC CAGCCGGAGT CATGACCTAG TGCTCGACCT GCGGAACCTG 
CTGAGCCGTT CGAGCGCCAG TATACAGGGC ATGGTGGAGA GGGCGGCTCG ACTGAATGGC 
ATTCTCGACC GGCGGCTGGT CGATGATGTG CTGGCCAAGG TGACGAGCAT GCTGCCCTCC 
ATGCGCGATG TCCGAGTCAC GTTGGAGGAG TCGGCCACAC AGATCGGTCG TGTCCAGCTG 
CAAAATTATC AGTTCGAAGT TTCGCTAACC GGTGCAGCAG GTTCCGTGCC CACAGGCGCC 
AATGTCAAGG TGATTCCAAC TATTACACCA GGTCTTCTTC GTCCGCTCTT CTCACAACAA 
CAGCTAAATC AGATACGTGG CTTTAAGACG GATCGTTCCA TTGAGGCGGA ACAAAAGCGC 
AATCCCACAA TGACCTCCCG ACTAAAGAAT GCTCTGGCCA ACTCGCCCCA GCGTCTGGAT 
GGGGACACAC CCCTTCAGGC TGAAAAACTG AGACGGCTGC TGGCCAAATC CGAAGAGCAT 
GGATTTAATA AAGCCGAGAG CCTGAAAATT GCCTTTGCCG AGGGCTACTT GGCCGCCGCT 
AACTCGGAGG ACTCTCCAAA ATCAGGAAAA ACTATGAAAT ATCTGAAGAC TTTGCAGACT 
ATCGTCGTTA TCGTCGTGTT CTTGGGCATT TTCCTGAGCT TCTTTACAAC CTCCAATGGA 
TCCGTATTTC GCATACAACT GGGCAACCAG GTTGAAGTGG ACCCTGAGGA GATCAACGTC 
ACTTTTGAGG ATGTCAAGGG TTGCGACGAA GCCAAGCAGG AGCTCAAGGA GGTCGTTGAA 
TTCCTGAAGA GTCCGGAAAA GTTCTCAAAT TTGGGCGGAA AGCTTCCAAA GGGAGTGCTC 
CTGGTTGGCC CGCCGGGTAC TGGCAAAACA CTGCTGGCCC GTGCCGTCGC CGGTGAAGCC 
AAAGTTCCTT TCTTCCATGC TGCCGGACCG GAGTTCGACG AGGTGCTCGT AGGCCAAGGA 
GCCAGACGAG TGCGTGATCT GTTCAAGGCA GCAAAGGCCC GTGCCCCTTG TGTTATCTTC 
ATTGACGAAA TCGACTCGGT GGGCGCCAAG CGAACCAACT CTGTGCTGCA TCCTTACGCC 
AATCAGACCA TCAATCAGCT GCTCTCCGAG ATGGATGGCT TCCATCAGAA CGCTGGCGTC 
ATTGTTTTGG GAGCCACCAA TCGTCGCGAC GATTTGGATC AGGCTCTGCT GCGTCCCGGC 
CGATTTGATG TAGAAGTCAT GGTGTCGACG CCCGATTTCA CTGGACGTAA GGAGATATTG 
TCTCTGTATC TGACGAAGAT TTTGCACGAT GAAATAGACT TGGACATGCT GGCGCGCGGC 
ACCTCTGGTT TTACTGGAGC GGATCTGGAA AACATGATTA ACCAAGCGGC GTTAAGAGCT 
GCCATCGATG GAGCCGAAAC GGTCAGCATG AAGCATTTGG AAACGGCACG AGACAAAGTA 
CTTATGGGAC CCGAGCGCAA GGCTCGTCTA CCGGACGAGG AGGCCAACAC CATAACCGCT 
TACCACGAGG GCGGCCACGC GATTGTGGCC TTCTACACCA AGGAGTCGCA TCCGCTGCAC 
AAGGTGACCA TCATGCCCCG CGGACCCTCC CTTGGACACA CCGCCTACAT ACCGGAGAAG 
GAGCGTTACC ATGTCACAAA GGCACAGCTG CTGGCCATGA TGGACACCAT GATGGGCGGT 
CGTGCTGCTG AGGAACTGGT CTTCGGCACG GACAAGATTA CGTCGGGAGC CAGCAGTGAT 
CTCAAGCAGG CAACCTCGAT CGCGACGCAT ATGGTCAGGG ACTGGGGCAT GTCGGACAAG 
GTCGGTCTTC GCACCATCGA GGCGTCAAAG GGTCTGGGCA CCGGTGACAC CCTTGGTCCC 
AACACCATTG AAGCCGTGGA CGCGGAGATC AAGCGCATCC TCAGCGACAG CTACGAGCGG 
GCGAAGGCTA TCCTGCGAAA ACACACCAGG GAGCACAAAG CGCTTGCGGA GGCGCTTCTC 
AAGTACGAGA CTCTTGACGC CGACGACATC AAGGCAATAC TGAATGAAAG CCAGACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

YME1L ( NM_001259544.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246473.1 Drosophila melanogaster YME1 like ATPase (YME1L), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa10976
Clone ID Related Accession (Same CDS sequence)	NM_001259544.2
Accession Version	NM_001259544.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	YME1 like ATPase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001259544.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTTCCA CCACCACACA CTCCGTGCCC TACCTGTACC TAGGCAACTT CAGCAGAAAG 
CCGCACTACT ACTCCGTTAA TCGAACCAAG CTGCACGGGA GCGCGGGAGC TGCCCGGTTG 
TCCAAGTCCA CGTCCACGTC CAGCCGGAGT CATGACCTAG TGCTCGACCT GCGGAACCTG 
CTGAGCCGTT CGAGCGCCAG TATACAGGGC ATGGTGGAGA GGGCGGCTCG ACTGAATGGC 
ATTCTCGACC GGCGGCTGGT CGATGATGTG CTGGCCAAGG TGACGAGCAT GCTGCCCTCC 
ATGCGCGATG TCCGAGTCAC GTTGGAGGAG TCGGCCACAC AGATCGGTCG TGTCCAGCTG 
CAAAATTATC AGTTCGAAGT TTCGCTAACC GGTGCAGCAG GTTCCGTGCC CACAGGCGCC 
AATGTCAAGG TGATTCCAAC TATTACACCA GGTCTTCTTC GTCCGCTCTT CTCACAACAA 
CAGCTAAATC AGATACGTGG CTTTAAGACG GATCGTTCCA TTGAGGCGGA ACAAAAGCGC 
AATCCCACAA TGACCTCCCG ACTAAAGAAT GCTCTGGCCA ACTCGCCCCA GCGTCTGGAT 
GGGGACACAC CCCTTCAGGC TGAAAAACTG AGACGGCTGC TGGCCAAATC CGAAGAGCAT 
GGATTTAATA AAGCCGAGAG CCTGAAAATT GCCTTTGCCG AGGGCTACTT GGCCGCCGCT 
AACTCGGAGG ACTCTCCAAA ATCAGGAAAA ACTATGAAAT ATCTGAAGAC TTTGCAGACT 
ATCGTCGTTA TCGTCGTGTT CTTGGGCATT TTCCTGAGCT TCTTTACAAC CTCCAATGGA 
TCCGTATTTC GCAGCATACA ACTGGGCAAC CAGGTTGAAG TGGACCCTGA GGAGATCAAC 
GTCACTTTTG AGGATGTCAA GGGTTGCGAC GAAGCCAAGC AGGAGCTCAA GGAGGTCGTT 
GAATTCCTGA AGAGTCCGGA AAAGTTCTCA AATTTGGGCG GAAAGCTTCC AAAGGGAGTG 
CTCCTGGTTG GCCCGCCGGG TACTGGCAAA ACACTGCTGG CCCGTGCCGT CGCCGGTGAA 
GCCAAAGTTC CTTTCTTCCA TGCTGCCGGA CCGGAGTTCG ACGAGGTGCT CGTAGGCCAA 
GGAGCCAGAC GAGTGCGTGA TCTGTTCAAG GCAGCAAAGG CCCGTGCCCC TTGTGTTATC 
TTCATTGACG AAATCGACTC GGTGGGCGCC AAGCGAACCA ACTCTGTGCT GCATCCTTAC 
GCCAATCAGA CCATCAATCA GCTGCTCTCC GAGATGGATG GCTTCCATCA GAACGCTGGC 
GTCATTGTTT TGGGAGCCAC CAATCGTCGC GACGATTTGG ATCAGGCTCT GCTGCGTCCC 
GGCCGATTTG ATGTAGAAGT CATGGTGTCG ACGCCCGATT TCACTGGACG TAAGGAGATA 
TTGTCTCTGT ATCTGACGAA GATTTTGCAC GATGAAATAG ACTTGGACAT GCTGGCGCGC 
GGCACCTCTG GTTTTACTGG AGCGGATCTG GAAAACATGA TTAACCAAGC GGCGTTAAGA 
GCTGCCATCG ATGGAGCCGA AACGGTCAGC ATGAAGCATT TGGAAACGGC ACGAGACAAA 
GTACTTATGG GACCCGAGCG CAAGGCTCGT CTACCGGACG AGGAGGCCAA CACCATAACC 
GCTTACCACG AGGGCGGCCA CGCGATTGTG GCCTTCTACA CCAAGGAGTC GCATCCGCTG 
CACAAGGTGA CCATCATGCC CCGCGGACCC TCCCTTGGAC ACACCGCCTA CATACCGGAG 
AAGGAGCGTT ACCATGTCAC AAAGGCACAG CTGCTGGCCA TGATGGACAC CATGATGGGC 
GGTCGTGCTG CTGAGGAACT GGTCTTCGGC ACGGACAAGA TTACGTCGGG AGCCAGCAGT 
GATCTCAAGC AGGCAACCTC GATCGCGACG CATATGGTCA GGGACTGGGG CATGTCGGAC 
AAGGTCGGTC TTCGCACCAT CGAGGCGTCA AAGGGTCTGG GCACCGGTGA CACCCTTGGT 
CCCAACACCA TTGAAGCCGT GGACGCGGAG ATCAAGCGCA TCCTCAGCGA CAGCTACGAG 
CGGGCGAAGG CTATCCTGCG AAAACACACC AGGGAGCACA AAGCGCTTGC GGAGGCGCTT 
CTCAAGTACG AGACTCTTGA CGCCGACGAC ATCAAGGCAA TACTGAATGA AAGCCAGACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246473.1
CDS	107..2329
Translation	MFSTTTHSVPYLYLGNFSRKPHYYSVNRTKLHGSAGAARLSKSTSTSSRSHDLVLDLRNLLSRSSASIQGMVERAARLNGILDRRLVDDVLAKVTSMLPSMRDVRVTLEESATQIGRVQLQNYQFEVSLTGAAGSVPTGANVKVIPTITPGLLRPLFSQQQLNQIRGFKTDRSIEAEQKRNPTMTSRLKNALANSPQRLDGDTPLQAEKLRRLLAKSEEHGFNKAESLKIAFAEGYLAAANSEDSPKSGKTMKYLKTLQTIVVIVVFLGIFLSFFTTSNGSVFRSIQLGNQVEVDPEEINVTFEDVKGCDEAKQELKEVVEFLKSPEKFSNLGGKLPKGVLLVGPPGTGKTLLARAVAGEAKVPFFHAAGPEFDEVLVGQGARRVRDLFKAAKARAPCVIFIDEIDSVGAKRTNSVLHPYANQTINQLLSEMDGFHQNAGVIVLGATNRRDDLDQALLRPGRFDVEVMVSTPDFTGRKEILSLYLTKILHDEIDLDMLARGTSGFTGADLENMINQAALRAAIDGAETVSMKHLETARDKVLMGPERKARLPDEEANTITAYHEGGHAIVAFYTKESHPLHKVTIMPRGPSLGHTAYIPEKERYHVTKAQLLAMMDTMMGGRAAEELVFGTDKITSGASSDLKQATSIATHMVRDWGMSDKVGLRTIEASKGLGTGDTLGPNTIEAVDAEIKRILSDSYERAKAILRKHTREHKALAEALLKYETLDADDIKAILNESQT

Target ORF information:

RefSeq Version	NM_001259544.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster YME1 like ATPase (YME1L), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259544.2

ORF Insert Sequence:

ATGTTTTCCA CCACCACACA CTCCGTGCCC TACCTGTACC TAGGCAACTT CAGCAGAAAG 
CCGCACTACT ACTCCGTTAA TCGAACCAAG CTGCACGGGA GCGCGGGAGC TGCCCGGTTG 
TCCAAGTCCA CGTCCACGTC CAGCCGGAGT CATGACCTAG TGCTCGACCT GCGGAACCTG 
CTGAGCCGTT CGAGCGCCAG TATACAGGGC ATGGTGGAGA GGGCGGCTCG ACTGAATGGC 
ATTCTCGACC GGCGGCTGGT CGATGATGTG CTGGCCAAGG TGACGAGCAT GCTGCCCTCC 
ATGCGCGATG TCCGAGTCAC GTTGGAGGAG TCGGCCACAC AGATCGGTCG TGTCCAGCTG 
CAAAATTATC AGTTCGAAGT TTCGCTAACC GGTGCAGCAG GTTCCGTGCC CACAGGCGCC 
AATGTCAAGG TGATTCCAAC TATTACACCA GGTCTTCTTC GTCCGCTCTT CTCACAACAA 
CAGCTAAATC AGATACGTGG CTTTAAGACG GATCGTTCCA TTGAGGCGGA ACAAAAGCGC 
AATCCCACAA TGACCTCCCG ACTAAAGAAT GCTCTGGCCA ACTCGCCCCA GCGTCTGGAT 
GGGGACACAC CCCTTCAGGC TGAAAAACTG AGACGGCTGC TGGCCAAATC CGAAGAGCAT 
GGATTTAATA AAGCCGAGAG CCTGAAAATT GCCTTTGCCG AGGGCTACTT GGCCGCCGCT 
AACTCGGAGG ACTCTCCAAA ATCAGGAAAA ACTATGAAAT ATCTGAAGAC TTTGCAGACT 
ATCGTCGTTA TCGTCGTGTT CTTGGGCATT TTCCTGAGCT TCTTTACAAC CTCCAATGGA 
TCCGTATTTC GCAGCATACA ACTGGGCAAC CAGGTTGAAG TGGACCCTGA GGAGATCAAC 
GTCACTTTTG AGGATGTCAA GGGTTGCGAC GAAGCCAAGC AGGAGCTCAA GGAGGTCGTT 
GAATTCCTGA AGAGTCCGGA AAAGTTCTCA AATTTGGGCG GAAAGCTTCC AAAGGGAGTG 
CTCCTGGTTG GCCCGCCGGG TACTGGCAAA ACACTGCTGG CCCGTGCCGT CGCCGGTGAA 
GCCAAAGTTC CTTTCTTCCA TGCTGCCGGA CCGGAGTTCG ACGAGGTGCT CGTAGGCCAA 
GGAGCCAGAC GAGTGCGTGA TCTGTTCAAG GCAGCAAAGG CCCGTGCCCC TTGTGTTATC 
TTCATTGACG AAATCGACTC GGTGGGCGCC AAGCGAACCA ACTCTGTGCT GCATCCTTAC 
GCCAATCAGA CCATCAATCA GCTGCTCTCC GAGATGGATG GCTTCCATCA GAACGCTGGC 
GTCATTGTTT TGGGAGCCAC CAATCGTCGC GACGATTTGG ATCAGGCTCT GCTGCGTCCC 
GGCCGATTTG ATGTAGAAGT CATGGTGTCG ACGCCCGATT TCACTGGACG TAAGGAGATA 
TTGTCTCTGT ATCTGACGAA GATTTTGCAC GATGAAATAG ACTTGGACAT GCTGGCGCGC 
GGCACCTCTG GTTTTACTGG AGCGGATCTG GAAAACATGA TTAACCAAGC GGCGTTAAGA 
GCTGCCATCG ATGGAGCCGA AACGGTCAGC ATGAAGCATT TGGAAACGGC ACGAGACAAA 
GTACTTATGG GACCCGAGCG CAAGGCTCGT CTACCGGACG AGGAGGCCAA CACCATAACC 
GCTTACCACG AGGGCGGCCA CGCGATTGTG GCCTTCTACA CCAAGGAGTC GCATCCGCTG 
CACAAGGTGA CCATCATGCC CCGCGGACCC TCCCTTGGAC ACACCGCCTA CATACCGGAG 
AAGGAGCGTT ACCATGTCAC AAAGGCACAG CTGCTGGCCA TGATGGACAC CATGATGGGC 
GGTCGTGCTG CTGAGGAACT GGTCTTCGGC ACGGACAAGA TTACGTCGGG AGCCAGCAGT 
GATCTCAAGC AGGCAACCTC GATCGCGACG CATATGGTCA GGGACTGGGG CATGTCGGAC 
AAGGTCGGTC TTCGCACCAT CGAGGCGTCA AAGGGTCTGG GCACCGGTGA CACCCTTGGT 
CCCAACACCA TTGAAGCCGT GGACGCGGAG ATCAAGCGCA TCCTCAGCGA CAGCTACGAG 
CGGGCGAAGG CTATCCTGCG AAAACACACC AGGGAGCACA AAGCGCTTGC GGAGGCGCTT 
CTCAAGTACG AGACTCTTGA CGCCGACGAC ATCAAGGCAA TACTGAATGA AAGCCAGACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

YME1L ( NM_166568.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726263.1 Drosophila melanogaster YME1 like ATPase (YME1L), transcript variant B, mRNA.

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CloneID	OFa12603
Clone ID Related Accession (Same CDS sequence)	NM_166568.3
Accession Version	NM_166568.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	YME1 like ATPase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_166568.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTTCCA CCACCACACA CTCCGTGCCC TACCTGTACC TAGGCAACTT CAGCAGAAAG 
CCGCACTACT ACTCCGTTAA TCGAACCAAG CTGCACGGGA GCGCGGGAGC TGCCCGGTTG 
TCCAAGTCCA CGTCCACGTC CAGCCGGAGT CATGACCTAG TGCTCGACCT GCGGAACCTG 
CTGAGCCGTT CGAGCGCCAG TATACAGGGC ATGGTGGAGA GGGCGGCTCG ACTGAATGGC 
ATTCTCGACC GGCGGCTGGT CGATGATGTG CTGGCCAAGG TGACGAGCAT GCTGCCCTCC 
ATGCGCGATG TCCGAGTCAC GTTGGAGGAG TCGGCCACAC AGATCGGTCG TGTCCAGCTG 
CAAAATTATC AGTTCGAAGT TTCGCTAACC GGTGCAGCAG GTTCCGTGCC CACAGGCGCC 
AATGTCAAGG TGATTCCAAC TATTACACCA GGTCTTCTTC GTCCGCTCTT CTCACAACAA 
CAGCTAAATC AGATACGTGG CTTTAAGACG GATCGTTCCA TTGAGGCGGA ACAAAAGCGC 
AATCCCACAA TGACCTCCCG ACTAAAGAAT GCTCTGGCCA ACTCGCCCCA GCGTCTGGAT 
GGGGACACAC CCCTTCAGGC TGAAAAACTG AGACGGCTGC TGGCCAAATC CGAAGAGCAT 
GGATTTAATA AAGCCGAGAG CCTGAAAATT GCCTTTGCCG AGGGCTACTT GGCCGCCGCT 
AACTCGGAGG ACTCTCCAAA ATCAGGAAAA ACTATGAAAT ATCTGAAGAC TATCGTCGTT 
ATCGTCGTGT TCTTGGGCAT TTTCCTGAGC TTCTTTACAA CCTCCAATGG ATCCGTATTT 
CGCATACAAC TGGGCAACCA GGTTGAAGTG GACCCTGAGG AGATCAACGT CACTTTTGAG 
GATGTCAAGG GTTGCGACGA AGCCAAGCAG GAGCTCAAGG AGGTCGTTGA ATTCCTGAAG 
AGTCCGGAAA AGTTCTCAAA TTTGGGCGGA AAGCTTCCAA AGGGAGTGCT CCTGGTTGGC 
CCGCCGGGTA CTGGCAAAAC ACTGCTGGCC CGTGCCGTCG CCGGTGAAGC CAAAGTTCCT 
TTCTTCCATG CTGCCGGACC GGAGTTCGAC GAGGTGCTCG TAGGCCAAGG AGCCAGACGA 
GTGCGTGATC TGTTCAAGGC AGCAAAGGCC CGTGCCCCTT GTGTTATCTT CATTGACGAA 
ATCGACTCGG TGGGCGCCAA GCGAACCAAC TCTGTGCTGC ATCCTTACGC CAATCAGACC 
ATCAATCAGC TGCTCTCCGA GATGGATGGC TTCCATCAGA ACGCTGGCGT CATTGTTTTG 
GGAGCCACCA ATCGTCGCGA CGATTTGGAT CAGGCTCTGC TGCGTCCCGG CCGATTTGAT 
GTAGAAGTCA TGGTGTCGAC GCCCGATTTC ACTGGACGTA AGGAGATATT GTCTCTGTAT 
CTGACGAAGA TTTTGCACGA TGAAATAGAC TTGGACATGC TGGCGCGCGG CACCTCTGGT 
TTTACTGGAG CGGATCTGGA AAACATGATT AACCAAGCGG CGTTAAGAGC TGCCATCGAT 
GGAGCCGAAA CGGTCAGCAT GAAGCATTTG GAAACGGCAC GAGACAAAGT ACTTATGGGA 
CCCGAGCGCA AGGCTCGTCT ACCGGACGAG GAGGCCAACA CCATAACCGC TTACCACGAG 
GGCGGCCACG CGATTGTGGC CTTCTACACC AAGGAGTCGC ATCCGCTGCA CAAGGTGACC 
ATCATGCCCC GCGGACCCTC CCTTGGACAC ACCGCCTACA TACCGGAGAA GGAGCGTTAC 
CATGTCACAA AGGCACAGCT GCTGGCCATG ATGGACACCA TGATGGGCGG TCGTGCTGCT 
GAGGAACTGG TCTTCGGCAC GGACAAGATT ACGTCGGGAG CCAGCAGTGA TCTCAAGCAG 
GCAACCTCGA TCGCGACGCA TATGGTCAGG GACTGGGGCA TGTCGGACAA GGTCGGTCTT 
CGCACCATCG AGGCGTCAAA GGGTCTGGGC ACCGGTGACA CCCTTGGTCC CAACACCATT 
GAAGCCGTGG ACGCGGAGAT CAAGCGCATC CTCAGCGACA GCTACGAGCG GGCGAAGGCT 
ATCCTGCGAA AACACACCAG GGAGCACAAA GCGCTTGCGG AGGCGCTTCT CAAGTACGAG 
ACTCTTGACG CCGACGACAT CAAGGCAATA CTGAATGAAA GCCAGACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726263.1
CDS	107..2317
Translation	MFSTTTHSVPYLYLGNFSRKPHYYSVNRTKLHGSAGAARLSKSTSTSSRSHDLVLDLRNLLSRSSASIQGMVERAARLNGILDRRLVDDVLAKVTSMLPSMRDVRVTLEESATQIGRVQLQNYQFEVSLTGAAGSVPTGANVKVIPTITPGLLRPLFSQQQLNQIRGFKTDRSIEAEQKRNPTMTSRLKNALANSPQRLDGDTPLQAEKLRRLLAKSEEHGFNKAESLKIAFAEGYLAAANSEDSPKSGKTMKYLKTIVVIVVFLGIFLSFFTTSNGSVFRIQLGNQVEVDPEEINVTFEDVKGCDEAKQELKEVVEFLKSPEKFSNLGGKLPKGVLLVGPPGTGKTLLARAVAGEAKVPFFHAAGPEFDEVLVGQGARRVRDLFKAAKARAPCVIFIDEIDSVGAKRTNSVLHPYANQTINQLLSEMDGFHQNAGVIVLGATNRRDDLDQALLRPGRFDVEVMVSTPDFTGRKEILSLYLTKILHDEIDLDMLARGTSGFTGADLENMINQAALRAAIDGAETVSMKHLETARDKVLMGPERKARLPDEEANTITAYHEGGHAIVAFYTKESHPLHKVTIMPRGPSLGHTAYIPEKERYHVTKAQLLAMMDTMMGGRAAEELVFGTDKITSGASSDLKQATSIATHMVRDWGMSDKVGLRTIEASKGLGTGDTLGPNTIEAVDAEIKRILSDSYERAKAILRKHTREHKALAEALLKYETLDADDIKAILNESQT

Target ORF information:

RefSeq Version	NM_166568.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster YME1 like ATPase (YME1L), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166568.3

ORF Insert Sequence:

ATGTTTTCCA CCACCACACA CTCCGTGCCC TACCTGTACC TAGGCAACTT CAGCAGAAAG 
CCGCACTACT ACTCCGTTAA TCGAACCAAG CTGCACGGGA GCGCGGGAGC TGCCCGGTTG 
TCCAAGTCCA CGTCCACGTC CAGCCGGAGT CATGACCTAG TGCTCGACCT GCGGAACCTG 
CTGAGCCGTT CGAGCGCCAG TATACAGGGC ATGGTGGAGA GGGCGGCTCG ACTGAATGGC 
ATTCTCGACC GGCGGCTGGT CGATGATGTG CTGGCCAAGG TGACGAGCAT GCTGCCCTCC 
ATGCGCGATG TCCGAGTCAC GTTGGAGGAG TCGGCCACAC AGATCGGTCG TGTCCAGCTG 
CAAAATTATC AGTTCGAAGT TTCGCTAACC GGTGCAGCAG GTTCCGTGCC CACAGGCGCC 
AATGTCAAGG TGATTCCAAC TATTACACCA GGTCTTCTTC GTCCGCTCTT CTCACAACAA 
CAGCTAAATC AGATACGTGG CTTTAAGACG GATCGTTCCA TTGAGGCGGA ACAAAAGCGC 
AATCCCACAA TGACCTCCCG ACTAAAGAAT GCTCTGGCCA ACTCGCCCCA GCGTCTGGAT 
GGGGACACAC CCCTTCAGGC TGAAAAACTG AGACGGCTGC TGGCCAAATC CGAAGAGCAT 
GGATTTAATA AAGCCGAGAG CCTGAAAATT GCCTTTGCCG AGGGCTACTT GGCCGCCGCT 
AACTCGGAGG ACTCTCCAAA ATCAGGAAAA ACTATGAAAT ATCTGAAGAC TATCGTCGTT 
ATCGTCGTGT TCTTGGGCAT TTTCCTGAGC TTCTTTACAA CCTCCAATGG ATCCGTATTT 
CGCATACAAC TGGGCAACCA GGTTGAAGTG GACCCTGAGG AGATCAACGT CACTTTTGAG 
GATGTCAAGG GTTGCGACGA AGCCAAGCAG GAGCTCAAGG AGGTCGTTGA ATTCCTGAAG 
AGTCCGGAAA AGTTCTCAAA TTTGGGCGGA AAGCTTCCAA AGGGAGTGCT CCTGGTTGGC 
CCGCCGGGTA CTGGCAAAAC ACTGCTGGCC CGTGCCGTCG CCGGTGAAGC CAAAGTTCCT 
TTCTTCCATG CTGCCGGACC GGAGTTCGAC GAGGTGCTCG TAGGCCAAGG AGCCAGACGA 
GTGCGTGATC TGTTCAAGGC AGCAAAGGCC CGTGCCCCTT GTGTTATCTT CATTGACGAA 
ATCGACTCGG TGGGCGCCAA GCGAACCAAC TCTGTGCTGC ATCCTTACGC CAATCAGACC 
ATCAATCAGC TGCTCTCCGA GATGGATGGC TTCCATCAGA ACGCTGGCGT CATTGTTTTG 
GGAGCCACCA ATCGTCGCGA CGATTTGGAT CAGGCTCTGC TGCGTCCCGG CCGATTTGAT 
GTAGAAGTCA TGGTGTCGAC GCCCGATTTC ACTGGACGTA AGGAGATATT GTCTCTGTAT 
CTGACGAAGA TTTTGCACGA TGAAATAGAC TTGGACATGC TGGCGCGCGG CACCTCTGGT 
TTTACTGGAG CGGATCTGGA AAACATGATT AACCAAGCGG CGTTAAGAGC TGCCATCGAT 
GGAGCCGAAA CGGTCAGCAT GAAGCATTTG GAAACGGCAC GAGACAAAGT ACTTATGGGA 
CCCGAGCGCA AGGCTCGTCT ACCGGACGAG GAGGCCAACA CCATAACCGC TTACCACGAG 
GGCGGCCACG CGATTGTGGC CTTCTACACC AAGGAGTCGC ATCCGCTGCA CAAGGTGACC 
ATCATGCCCC GCGGACCCTC CCTTGGACAC ACCGCCTACA TACCGGAGAA GGAGCGTTAC 
CATGTCACAA AGGCACAGCT GCTGGCCATG ATGGACACCA TGATGGGCGG TCGTGCTGCT 
GAGGAACTGG TCTTCGGCAC GGACAAGATT ACGTCGGGAG CCAGCAGTGA TCTCAAGCAG 
GCAACCTCGA TCGCGACGCA TATGGTCAGG GACTGGGGCA TGTCGGACAA GGTCGGTCTT 
CGCACCATCG AGGCGTCAAA GGGTCTGGGC ACCGGTGACA CCCTTGGTCC CAACACCATT 
GAAGCCGTGG ACGCGGAGAT CAAGCGCATC CTCAGCGACA GCTACGAGCG GGCGAAGGCT 
ATCCTGCGAA AACACACCAG GGAGCACAAA GCGCTTGCGG AGGCGCTTCT CAAGTACGAG 
ACTCTTGACG CCGACGACAT CAAGGCAATA CTGAATGAAA GCCAGACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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YME1L cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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