CG13527 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG13527
Entrez Gene ID	37615
Full Name	CG13527 gene product from transcript CG13527-RB
Synonyms	Dmel\CG13527,SPH182
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 59A4-59A4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001299815.1	NP_001286744.1	uncharacterized protein, isoform B
NM_137872.5	NP_611716.3	uncharacterized protein, isoform A

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG13527	NP_611716.3
Drosophila melanogaster (fruit fly)	CG4477	NP_648295.1
Drosophila melanogaster (fruit fly)	CG34171	NP_001097175.2
Drosophila melanogaster (fruit fly)	CG15873	NP_611910.2
Drosophila melanogaster (fruit fly)	CG3795	NP_569939.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006087	XP_316143.4
Drosophila melanogaster (fruit fly)	CG18223	NP_649077.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG13527 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG13527 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa12580	NM_137872.5 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG13527), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa21920	NM_001299815.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG13527), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG13527 ( NM_137872.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611716.3 Drosophila melanogaster uncharacterized protein, transcript variant A (CG13527), mRNA.

Price & Availability↑

CloneID	OFa12580
Clone ID Related Accession (Same CDS sequence)	NM_137872.5
Accession Version	NM_137872.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137872.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAAAGA AATGCCAATA TGTAGCCATT TTAATAACAG TGATGGTAAT CCTTAGTGGA 
GCCCATCGGA TGAAGCGTTT ATCCTCGCCC AAATTTCATG GAGACGAAAC CCTCGAGTTG 
GCCAAGTACG TGGTGTCCAT CAGATCGAGA ACCCCAAACA AGTATTTTGG TGACAATCAC 
TATTGCGGAG GAGGGCTACT GTCCAATCAA TGGGTGATTA CTGCCGCCCA TTGTGTCATG 
GGACAATCCA AGATCATGTA CAAGGCTCGA TGGCTGCTGG TGGTGGCCGG ATCCCCGCAT 
AGGCTGCGCT ATACCCCCGG AAAATCGGTC TGTTCGCCAG TGTCCAGTCT GTACGTCCCA 
AAGAACTTCA CCATGCACAA CACCTTCAAC ATGGCCCTAA TGAAGCTTCA GGAGAAAATG 
CCCTCGAATG ACCCTCGAAT CGGATTCCTT CATCTGCCAA AAGAGGCGCC GAAAATTGGA 
ATCAGACACA CAGTCCTCGG CTGGGGAAGG ATGTATTTTG GCGGACCTTT GGCGGTGCAC 
ATATATCAAG TGGACGTGGT TCTCATGGAC AACGCGGTGT GTAAGACGTA CTTCCGACAC 
TACGGGGATG GAATGATGTG TGCTGGGAAC AATAATTGGA CAATAGACGC AGAACCGTGC 
AGCGGCGACA TCGGATCCCC ATTACTATCC GGAAAAGTGG TTGTCGGCAT AGTTGCCTAT 
CCGATTGGCT GCGGTTGCAC CAATATTCCG TCCGTCTATA CTGATGTATT TAGCGGCTTA 
AGGTGGATCA GGCATACGGC ATATGACTGG GCCAGCATCA CTAAGACAAA TCCAACCCTC 
ATGTTCATAC TGTTGTATGT ACTTCATAGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611716.3
CDS	99..971
Translation	MAKKCQYVAILITVMVILSGAHRMKRLSSPKFHGDETLELAKYVVSIRSRTPNKYFGDNHYCGGGLLSNQWVITAAHCVMGQSKIMYKARWLLVVAGSPHRLRYTPGKSVCSPVSSLYVPKNFTMHNTFNMALMKLQEKMPSNDPRIGFLHLPKEAPKIGIRHTVLGWGRMYFGGPLAVHIYQVDVVLMDNAVCKTYFRHYGDGMMCAGNNNWTIDAEPCSGDIGSPLLSGKVVVGIVAYPIGCGCTNIPSVYTDVFSGLRWIRHTAYDWASITKTNPTLMFILLYVLHR

Target ORF information:

RefSeq Version	NM_137872.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG13527), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137872.5

ORF Insert Sequence:

ATGGCAAAGA AATGCCAATA TGTAGCCATT TTAATAACAG TGATGGTAAT CCTTAGTGGA 
GCCCATCGGA TGAAGCGTTT ATCCTCGCCC AAATTTCATG GAGACGAAAC CCTCGAGTTG 
GCCAAGTACG TGGTGTCCAT CAGATCGAGA ACCCCAAACA AGTATTTTGG TGACAATCAC 
TATTGCGGAG GAGGGCTACT GTCCAATCAA TGGGTGATTA CTGCCGCCCA TTGTGTCATG 
GGACAATCCA AGATCATGTA CAAGGCTCGA TGGCTGCTGG TGGTGGCCGG ATCCCCGCAT 
AGGCTGCGCT ATACCCCCGG AAAATCGGTC TGTTCGCCAG TGTCCAGTCT GTACGTCCCA 
AAGAACTTCA CCATGCACAA CACCTTCAAC ATGGCCCTAA TGAAGCTTCA GGAGAAAATG 
CCCTCGAATG ACCCTCGAAT CGGATTCCTT CATCTGCCAA AAGAGGCGCC GAAAATTGGA 
ATCAGACACA CAGTCCTCGG CTGGGGAAGG ATGTATTTTG GCGGACCTTT GGCGGTGCAC 
ATATATCAAG TGGACGTGGT TCTCATGGAC AACGCGGTGT GTAAGACGTA CTTCCGACAC 
TACGGGGATG GAATGATGTG TGCTGGGAAC AATAATTGGA CAATAGACGC AGAACCGTGC 
AGCGGCGACA TCGGATCCCC ATTACTATCC GGAAAAGTGG TTGTCGGCAT AGTTGCCTAT 
CCGATTGGCT GCGGTTGCAC CAATATTCCG TCCGTCTATA CTGATGTATT TAGCGGCTTA 
AGGTGGATCA GGCATACGGC ATATGACTGG GCCAGCATCA CTAAGACAAA TCCAACCCTC 
ATGTTCATAC TGTTGTATGT ACTTCATAGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG13527 ( NM_001299815.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286744.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG13527), mRNA.

Price & Availability↑

CloneID	OFa21920
Clone ID Related Accession (Same CDS sequence)	NM_001299815.1
Accession Version	NM_001299815.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAAAGA AATGCCAATA TGTAGCCATT TTAATAACAG TGATGGTAAT CCTTAGTGGA 
GCCCATCGGA TGAAGCGTTT ATCCTCGCCC AAATTTCATG GAGACGAAAC CCTCGAGTTG 
GCCAAGTACG TGGTGTCCAT CAGATCGAGA ACCCCAAACA AGTATTTTGG TGACAATCAC 
TATTGCGGAG GAGGGCTACT GTCCAATCAA TGGGTGATTA CTGCCGCCCA TTGTGTCATG 
GGACAATCCA AGATCATGTA CAAGGCTCGA TGGCTGCTGG TGGTGGCCGG ATCCCCGCAT 
AGGCTGCGCT ATACCCCCGG AAAATCGGTC TGTTCGCCAG TGTCCAGTCT GTACGTCCCA 
AAGAACTTCA CCATGCACAA CACCTTCAAC ATGGCCCTAA TGAAGCTTCA GGAGAAAATG 
CCCTCGAATG ACCCTCGAAT CGGATTCCTT CATCTGCCAA AAGAGGCGCC GAAAATTGGA 
ATCAGACACA CAGTCCTCGG CTGGGGAAGG ATGTATTTTG GCGGACCTTT GGCGGTGCAC 
ATATATCAAG TGGACGTGGT TCTCATGGAC AACGCGGTGT GTAAGACGTA CTTCCGACAC 
TACGGGGATG GAATGATGTG TGCTGGGAAC AATAATTGGA CAATAGACGC AGAACCGTGC 
AGCGGCGACA TCGGATCCCC ATTACTATCC GGAAAAGTGG TTGTCGGCAT AGTTGCCTAT 
CCGATTGGCT GCGGTTGCAC CAATATTCCG TCCGTCTATA CTGATGTATT TAGCGGCTTA 
AGGTGGATCA GGCATACGGC ATATGACTGG GCCAGCATCA CTAAGACAAA TCCAACCCTC 
ATGTTCATAC TGTTGTATGT ACTTCATAGA CTAGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286744.1
CDS	99..977
Translation	MAKKCQYVAILITVMVILSGAHRMKRLSSPKFHGDETLELAKYVVSIRSRTPNKYFGDNHYCGGGLLSNQWVITAAHCVMGQSKIMYKARWLLVVAGSPHRLRYTPGKSVCSPVSSLYVPKNFTMHNTFNMALMKLQEKMPSNDPRIGFLHLPKEAPKIGIRHTVLGWGRMYFGGPLAVHIYQVDVVLMDNAVCKTYFRHYGDGMMCAGNNNWTIDAEPCSGDIGSPLLSGKVVVGIVAYPIGCGCTNIPSVYTDVFSGLRWIRHTAYDWASITKTNPTLMFILLYVLHRLG

Target ORF information:

RefSeq Version	NM_001299815.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG13527), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299815.1

ORF Insert Sequence:

ATGGCAAAGA AATGCCAATA TGTAGCCATT TTAATAACAG TGATGGTAAT CCTTAGTGGA 
GCCCATCGGA TGAAGCGTTT ATCCTCGCCC AAATTTCATG GAGACGAAAC CCTCGAGTTG 
GCCAAGTACG TGGTGTCCAT CAGATCGAGA ACCCCAAACA AGTATTTTGG TGACAATCAC 
TATTGCGGAG GAGGGCTACT GTCCAATCAA TGGGTGATTA CTGCCGCCCA TTGTGTCATG 
GGACAATCCA AGATCATGTA CAAGGCTCGA TGGCTGCTGG TGGTGGCCGG ATCCCCGCAT 
AGGCTGCGCT ATACCCCCGG AAAATCGGTC TGTTCGCCAG TGTCCAGTCT GTACGTCCCA 
AAGAACTTCA CCATGCACAA CACCTTCAAC ATGGCCCTAA TGAAGCTTCA GGAGAAAATG 
CCCTCGAATG ACCCTCGAAT CGGATTCCTT CATCTGCCAA AAGAGGCGCC GAAAATTGGA 
ATCAGACACA CAGTCCTCGG CTGGGGAAGG ATGTATTTTG GCGGACCTTT GGCGGTGCAC 
ATATATCAAG TGGACGTGGT TCTCATGGAC AACGCGGTGT GTAAGACGTA CTTCCGACAC 
TACGGGGATG GAATGATGTG TGCTGGGAAC AATAATTGGA CAATAGACGC AGAACCGTGC 
AGCGGCGACA TCGGATCCCC ATTACTATCC GGAAAAGTGG TTGTCGGCAT AGTTGCCTAT 
CCGATTGGCT GCGGTTGCAC CAATATTCCG TCCGTCTATA CTGATGTATT TAGCGGCTTA 
AGGTGGATCA GGCATACGGC ATATGACTGG GCCAGCATCA CTAAGACAAA TCCAACCCTC 
ATGTTCATAC TGTTGTATGT ACTTCATAGA CTAGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG13527 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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