Ppa cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ppa
Entrez Gene ID	37602
Full Name	Partner of paired
Synonyms	CG9952,Dmel\CG9952,FBXL14,I-55,PPA,ppa
General protein information	Preferred Names Partner of paired Names CG9952 gene product from transcript CG9952-RC CG9952-PA CG9952-PB CG9952-PC Ppa-PA Ppa-PB Ppa-PC partner of paired
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 59A1-59A2

mRNA and Protein(s)

mRNA	Protein	Name
NM_079088.3	NP_523812.1	partner of paired, isoform A
NM_001274209.2	NP_001261138.1	partner of paired, isoform B
NM_001299814.1	NP_001286743.1	partner of paired, isoform C

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Fbxl14	NP_001258205.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010289	XP_319486.4
Xenopus tropicalis (tropical clawed frog)	fbxl14	XP_004912788.1
	FBXL14	XP_001117874.2
Canis lupus familiaris (dog)	FBXL14	XP_005637418.1
Danio rerio (zebrafish)	fbxl14b	NP_001015043.1
Homo sapiens (human)	FBXL14	NP_689654.1
Mus musculus (house mouse)	Fbxl14	NP_598701.2
Danio rerio (zebrafish)	fbxl14a	NP_958890.1
Drosophila melanogaster (fruit fly)	ppa	NP_523812.1
Pan troglodytes (chimpanzee)	FBXL14	XP_003952131.1
Gallus gallus (chicken)	FBXL14	XP_003640464.2
Bos taurus (cattle)	FBXL14	NP_001069415.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The F box protein partner of paired (Ppa) mediates CenH3(CID) stability in Drosophila. Ppa depletion results in increased CenH3(CID) levels. Ppa physically interacts with CenH3(CID) through the CATD(CID) that mediates Ppa-dependent CenH3(CID) stability.
Title: The F box protein partner of paired regulates stability of Drosophila centromeric histone H3, CenH3(CID).

Concludes that patterning of ppa mRNA could reflect a constraint on ppa function that is not common among F-box genes.
Title: A conserved F-box gene with unusual transcript localization.

The following Ppa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa10968	NM_001274209.2 Latest version!	Drosophila melanogaster partner of paired, transcript variant B (Ppa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa12567	NM_001299814.1 Latest version!	Drosophila melanogaster partner of paired, transcript variant C (Ppa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa12567	NM_079088.3 Latest version!	Drosophila melanogaster partner of paired, transcript variant A (Ppa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ppa ( NM_001274209.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261138.1 Drosophila melanogaster partner of paired, transcript variant B (Ppa), mRNA.

Price & Availability↑

CloneID	OFa10968
Clone ID Related Accession (Same CDS sequence)	NM_001274209.2
Accession Version	NM_001274209.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	partner of paired, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001274209.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGACCA ACACGGAGCT GCTGCCCTTC CATCATCAAA CAATCGCGGT GTTGAACAAT 
CTGAATAGCC ACTGTGGCGG ATCCGGGAGC AACGGAGCGG CCACCAGTGG CGGCGCGGCG 
ATTGGCGGAG CAGGAGCCGG AGGAGCGCCC AGCTGGGCCA TGGACGAACT CTATCAACAG 
ACTGAGCTGC CCGGTCTGAC GCGAACCGGA GCCACCACCA CCCACCACCA CCTGCTGCGC 
TTCACGCCCT ACGCCCTGCA CCACCGACCG CCGCACCACC AGCTGCAGAC CTTGCCGCCG 
GCCCTCTACT TGCAGCATGC CGCAGCAGCT GCGGCGGCAG CGGCAGCAGC AGCGGCCCAC 
GCCCAGCACC ATCATCACCA TCACCACCAT CTCCCGCACA GACCAGCATC TCCGGAGAGC 
CCGCCGCCGG TGGAGGGCAC CCACATAAGC AACCTGTTCC CAGAACTCCT CGAGCAGATA 
TTCGAGCACC TACCAGTGAG GGATTTGGGT CGGGCAGCTC AGGTTTGCAC CGCATGGCGG 
GATGCGGCCT ATGCCAAGAG CGTTTGGAAG GGAGTCGAGG CCAAGCTGCA CCTTAAGCGT 
TCCAGTCCGA GCCTGTTCAA CTGCCTGGTC AAGCGCGGCA TCAAGAAGGT GCAGATCCTA 
TCGCTGCGCC GCTCGCTTAA GGATTTGGTC CTGGGCGTTC CGGCTCTGAC CTCTCTGAAC 
CTGAGCGGCT GCTTCAACGT GGCGGACATG AACCTGGGCC ATGCCTTTAG CGTGGATCTG 
CCAAACCTCA AGACACTGGA CCTCTCCCTC TGTAAACAGA TCACGGACAC GAGTCTGGGG 
CGCATCGCCC AGCATCTAAG GAATCTTGAG ACCCTGGAAC TGGGCGGCTG TTGCAACATC 
ACGAACACAG GACTGCTGCT CATTGCGTGG GGCCTGAAGA AGCTGAAGCA CCTCAATCTG 
CGCTCCTGCT GGCACATCAG CGACCAGGGA ATCGGACACC TGGCTGGTTT CTCGCGGGAA 
ACGGCCGAAG GCAACCTGCA GCTGGAGTAC TTGGGTCTGC AGGATTGCCA GCGGCTAAGC 
GACGAGGCTT TGGGCCACAT TGCCCAGGGC CTGACCTCGC TGAAGTCGAT CAACCTGAGC 
TTCTGCGTCT CGGTGACGGA CAGCGGACTA AAGCACCTGG CCCGGATGCC CAAGCTCGAG 
CAGCTGAACT TGCGCTCCTG CGACAACATC TCGGATATCG GAATGGCCTA CCTCACGGAA 
GGCGGCAGCG GGATCAACTC GCTGGACGTC AGTTTCTGCG ACAAGATCAG CGACCAGGCC 
CTGACACACA TAGCCCAGGG ACTCTACAGA CTGAGATCCC TGTCCCTCAA TCAGTGCCAG 
ATCACCGATC ACGGCATGCT AAAGATCGCC AAGGCATTGC ACGAGTTGGA GAACCTCAAT 
ATCGGACAGT GCAGCCGGAT AACGGACAAG GGCCTGCAAA CGCTGGCCGA GGATCTGACT 
AATCTCAAGA CCATCGATCT CTACGGCTGC ACGCAACTCA GTTCCAAGGG CATCGACATC 
ATCATGAAGC TGCCCAAGCT GCAGAAGCTC AACCTGGGCC TCTGGCTGGT CAGGTGATGC 
GTCCACCACG ACTGCAACGC CTGTGCGGCG AAGGAGGCCG CTCGCGGCTC TTATAATTCC 
CAACCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261138.1
CDS	267..1955
Translation	MATNTELLPFHHQTIAVLNNLNSHCGGSGSNGAATSGGAAIGGAGAGGAPSWAMDELYQQTELPGLTRTGATTTHHHLLRFTPYALHHRPPHHQLQTLPPALYLQHAAAAAAAAAAAAAHAQHHHHHHHHLPHRPASPESPPPVEGTHISNLFPELLEQIFEHLPVRDLGRAAQVCTAWRDAAYAKSVWKGVEAKLHLKRSSPSLFNCLVKRGIKKVQILSLRRSLKDLVLGVPALTSLNLSGCFNVADMNLGHAFSVDLPNLKTLDLSLCKQITDTSLGRIAQHLRNLETLELGGCCNITNTGLLLIAWGLKKLKHLNLRSCWHISDQGIGHLAGFSRETAEGNLQLEYLGLQDCQRLSDEALGHIAQGLTSLKSINLSFCVSVTDSGLKHLARMPKLEQLNLRSCDNISDIGMAYLTEGGSGINSLDVSFCDKISDQALTHIAQGLYRLRSLSLNQCQITDHGMLKIAKALHELENLNIGQCSRITDKGLQTLAEDLTNLKTIDLYGCTQLSSKGIDIIMKLPKLQKLNLGLWLVR*CVHHDCNACAAKEAARGSYNSQP

Target ORF information:

RefSeq Version	NM_001274209.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster partner of paired, transcript variant B (Ppa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274209.2

ORF Insert Sequence:

ATGGCGACCA ACACGGAGCT GCTGCCCTTC CATCATCAAA CAATCGCGGT GTTGAACAAT 
CTGAATAGCC ACTGTGGCGG ATCCGGGAGC AACGGAGCGG CCACCAGTGG CGGCGCGGCG 
ATTGGCGGAG CAGGAGCCGG AGGAGCGCCC AGCTGGGCCA TGGACGAACT CTATCAACAG 
ACTGAGCTGC CCGGTCTGAC GCGAACCGGA GCCACCACCA CCCACCACCA CCTGCTGCGC 
TTCACGCCCT ACGCCCTGCA CCACCGACCG CCGCACCACC AGCTGCAGAC CTTGCCGCCG 
GCCCTCTACT TGCAGCATGC CGCAGCAGCT GCGGCGGCAG CGGCAGCAGC AGCGGCCCAC 
GCCCAGCACC ATCATCACCA TCACCACCAT CTCCCGCACA GACCAGCATC TCCGGAGAGC 
CCGCCGCCGG TGGAGGGCAC CCACATAAGC AACCTGTTCC CAGAACTCCT CGAGCAGATA 
TTCGAGCACC TACCAGTGAG GGATTTGGGT CGGGCAGCTC AGGTTTGCAC CGCATGGCGG 
GATGCGGCCT ATGCCAAGAG CGTTTGGAAG GGAGTCGAGG CCAAGCTGCA CCTTAAGCGT 
TCCAGTCCGA GCCTGTTCAA CTGCCTGGTC AAGCGCGGCA TCAAGAAGGT GCAGATCCTA 
TCGCTGCGCC GCTCGCTTAA GGATTTGGTC CTGGGCGTTC CGGCTCTGAC CTCTCTGAAC 
CTGAGCGGCT GCTTCAACGT GGCGGACATG AACCTGGGCC ATGCCTTTAG CGTGGATCTG 
CCAAACCTCA AGACACTGGA CCTCTCCCTC TGTAAACAGA TCACGGACAC GAGTCTGGGG 
CGCATCGCCC AGCATCTAAG GAATCTTGAG ACCCTGGAAC TGGGCGGCTG TTGCAACATC 
ACGAACACAG GACTGCTGCT CATTGCGTGG GGCCTGAAGA AGCTGAAGCA CCTCAATCTG 
CGCTCCTGCT GGCACATCAG CGACCAGGGA ATCGGACACC TGGCTGGTTT CTCGCGGGAA 
ACGGCCGAAG GCAACCTGCA GCTGGAGTAC TTGGGTCTGC AGGATTGCCA GCGGCTAAGC 
GACGAGGCTT TGGGCCACAT TGCCCAGGGC CTGACCTCGC TGAAGTCGAT CAACCTGAGC 
TTCTGCGTCT CGGTGACGGA CAGCGGACTA AAGCACCTGG CCCGGATGCC CAAGCTCGAG 
CAGCTGAACT TGCGCTCCTG CGACAACATC TCGGATATCG GAATGGCCTA CCTCACGGAA 
GGCGGCAGCG GGATCAACTC GCTGGACGTC AGTTTCTGCG ACAAGATCAG CGACCAGGCC 
CTGACACACA TAGCCCAGGG ACTCTACAGA CTGAGATCCC TGTCCCTCAA TCAGTGCCAG 
ATCACCGATC ACGGCATGCT AAAGATCGCC AAGGCATTGC ACGAGTTGGA GAACCTCAAT 
ATCGGACAGT GCAGCCGGAT AACGGACAAG GGCCTGCAAA CGCTGGCCGA GGATCTGACT 
AATCTCAAGA CCATCGATCT CTACGGCTGC ACGCAACTCA GTTCCAAGGG CATCGACATC 
ATCATGAAGC TGCCCAAGCT GCAGAAGCTC AACCTGGGCC TCTGGCTGGT CAGGTGATGC 
GTCCACCACG ACTGCAACGC CTGTGCGGCG AAGGAGGCCG CTCGCGGCTC TTATAATTCC 
CAACCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppa ( NM_079088.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523812.1 Drosophila melanogaster partner of paired, transcript variant A (Ppa), mRNA.

Price & Availability↑

CloneID	OFa12567
Clone ID Related Accession (Same CDS sequence)	NM_079088.3 , NM_001299814.1
Accession Version	NM_079088.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	partner of paired, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_079088.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGACCA ACACGGAGCT GCTGCCCTTC CATCATCAAA CAATCGCGGT GTTGAACAAT 
CTGAATAGCC ACTGTGGCGG ATCCGGGAGC AACGGAGCGG CCACCAGTGG CGGCGCGGCG 
ATTGGCGGAG CAGGAGCCGG AGGAGCGCCC AGCTGGGCCA TGGACGAACT CTATCAACAG 
ACTGAGCTGC CCGGTCTGAC GCGAACCGGA GCCACCACCA CCCACCACCA CCTGCTGCGC 
TTCACGCCCT ACGCCCTGCA CCACCGACCG CCGCACCACC AGCTGCAGAC CTTGCCGCCG 
GCCCTCTACT TGCAGCATGC CGCAGCAGCT GCGGCGGCAG CGGCAGCAGC AGCGGCCCAC 
GCCCAGCACC ATCATCACCA TCACCACCAT CTCCCGCACA GACCAGCATC TCCGGAGAGC 
CCGCCGCCGG TGGAGGGCAC CCACATAAGC AACCTGTTCC CAGAACTCCT CGAGCAGATA 
TTCGAGCACC TACCAGTGAG GGATTTGGGT CGGGCAGCTC AGGTTTGCAC CGCATGGCGG 
GATGCGGCCT ATGCCAAGAG CGTTTGGAAG GGAGTCGAGG CCAAGCTGCA CCTTAAGCGT 
TCCAGTCCGA GCCTGTTCAA CTGCCTGGTC AAGCGCGGCA TCAAGAAGGT GCAGATCCTA 
TCGCTGCGCC GCTCGCTTAA GGATTTGGTC CTGGGCGTTC CGGCTCTGAC CTCTCTGAAC 
CTGAGCGGCT GCTTCAACGT GGCGGACATG AACCTGGGCC ATGCCTTTAG CGTGGATCTG 
CCAAACCTCA AGACACTGGA CCTCTCCCTC TGTAAACAGA TCACGGACAC GAGTCTGGGG 
CGCATCGCCC AGCATCTAAG GAATCTTGAG ACCCTGGAAC TGGGCGGCTG TTGCAACATC 
ACGAACACAG GACTGCTGCT CATTGCGTGG GGCCTGAAGA AGCTGAAGCA CCTCAATCTG 
CGCTCCTGCT GGCACATCAG CGACCAGGGA ATCGGACACC TGGCTGGTTT CTCGCGGGAA 
ACGGCCGAAG GCAACCTGCA GCTGGAGTAC TTGGGTCTGC AGGATTGCCA GCGGCTAAGC 
GACGAGGCTT TGGGCCACAT TGCCCAGGGC CTGACCTCGC TGAAGTCGAT CAACCTGAGC 
TTCTGCGTCT CGGTGACGGA CAGCGGACTA AAGCACCTGG CCCGGATGCC CAAGCTCGAG 
CAGCTGAACT TGCGCTCCTG CGACAACATC TCGGATATCG GAATGGCCTA CCTCACGGAA 
GGCGGCAGCG GGATCAACTC GCTGGACGTC AGTTTCTGCG ACAAGATCAG CGACCAGGCC 
CTGACACACA TAGCCCAGGG ACTCTACAGA CTGAGATCCC TGTCCCTCAA TCAGTGCCAG 
ATCACCGATC ACGGCATGCT AAAGATCGCC AAGGCATTGC ACGAGTTGGA GAACCTCAAT 
ATCGGACAGT GCAGCCGGAT AACGGACAAG GGCCTGCAAA CGCTGGCCGA GGATCTGACT 
AATCTCAAGA CCATCGATCT CTACGGCTGC ACGCAACTCA GTTCCAAGGG CATCGACATC 
ATCATGAAGC TGCCCAAGCT GCAGAAGCTC AACCTGGGCC TCTGGCTGGT CAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523812.1
CDS	267..1883
Translation	MATNTELLPFHHQTIAVLNNLNSHCGGSGSNGAATSGGAAIGGAGAGGAPSWAMDELYQQTELPGLTRTGATTTHHHLLRFTPYALHHRPPHHQLQTLPPALYLQHAAAAAAAAAAAAAHAQHHHHHHHHLPHRPASPESPPPVEGTHISNLFPELLEQIFEHLPVRDLGRAAQVCTAWRDAAYAKSVWKGVEAKLHLKRSSPSLFNCLVKRGIKKVQILSLRRSLKDLVLGVPALTSLNLSGCFNVADMNLGHAFSVDLPNLKTLDLSLCKQITDTSLGRIAQHLRNLETLELGGCCNITNTGLLLIAWGLKKLKHLNLRSCWHISDQGIGHLAGFSRETAEGNLQLEYLGLQDCQRLSDEALGHIAQGLTSLKSINLSFCVSVTDSGLKHLARMPKLEQLNLRSCDNISDIGMAYLTEGGSGINSLDVSFCDKISDQALTHIAQGLYRLRSLSLNQCQITDHGMLKIAKALHELENLNIGQCSRITDKGLQTLAEDLTNLKTIDLYGCTQLSSKGIDIIMKLPKLQKLNLGLWLVR

Target ORF information:

RefSeq Version	NM_079088.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster partner of paired, transcript variant A (Ppa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079088.3

ORF Insert Sequence:

ATGGCGACCA ACACGGAGCT GCTGCCCTTC CATCATCAAA CAATCGCGGT GTTGAACAAT 
CTGAATAGCC ACTGTGGCGG ATCCGGGAGC AACGGAGCGG CCACCAGTGG CGGCGCGGCG 
ATTGGCGGAG CAGGAGCCGG AGGAGCGCCC AGCTGGGCCA TGGACGAACT CTATCAACAG 
ACTGAGCTGC CCGGTCTGAC GCGAACCGGA GCCACCACCA CCCACCACCA CCTGCTGCGC 
TTCACGCCCT ACGCCCTGCA CCACCGACCG CCGCACCACC AGCTGCAGAC CTTGCCGCCG 
GCCCTCTACT TGCAGCATGC CGCAGCAGCT GCGGCGGCAG CGGCAGCAGC AGCGGCCCAC 
GCCCAGCACC ATCATCACCA TCACCACCAT CTCCCGCACA GACCAGCATC TCCGGAGAGC 
CCGCCGCCGG TGGAGGGCAC CCACATAAGC AACCTGTTCC CAGAACTCCT CGAGCAGATA 
TTCGAGCACC TACCAGTGAG GGATTTGGGT CGGGCAGCTC AGGTTTGCAC CGCATGGCGG 
GATGCGGCCT ATGCCAAGAG CGTTTGGAAG GGAGTCGAGG CCAAGCTGCA CCTTAAGCGT 
TCCAGTCCGA GCCTGTTCAA CTGCCTGGTC AAGCGCGGCA TCAAGAAGGT GCAGATCCTA 
TCGCTGCGCC GCTCGCTTAA GGATTTGGTC CTGGGCGTTC CGGCTCTGAC CTCTCTGAAC 
CTGAGCGGCT GCTTCAACGT GGCGGACATG AACCTGGGCC ATGCCTTTAG CGTGGATCTG 
CCAAACCTCA AGACACTGGA CCTCTCCCTC TGTAAACAGA TCACGGACAC GAGTCTGGGG 
CGCATCGCCC AGCATCTAAG GAATCTTGAG ACCCTGGAAC TGGGCGGCTG TTGCAACATC 
ACGAACACAG GACTGCTGCT CATTGCGTGG GGCCTGAAGA AGCTGAAGCA CCTCAATCTG 
CGCTCCTGCT GGCACATCAG CGACCAGGGA ATCGGACACC TGGCTGGTTT CTCGCGGGAA 
ACGGCCGAAG GCAACCTGCA GCTGGAGTAC TTGGGTCTGC AGGATTGCCA GCGGCTAAGC 
GACGAGGCTT TGGGCCACAT TGCCCAGGGC CTGACCTCGC TGAAGTCGAT CAACCTGAGC 
TTCTGCGTCT CGGTGACGGA CAGCGGACTA AAGCACCTGG CCCGGATGCC CAAGCTCGAG 
CAGCTGAACT TGCGCTCCTG CGACAACATC TCGGATATCG GAATGGCCTA CCTCACGGAA 
GGCGGCAGCG GGATCAACTC GCTGGACGTC AGTTTCTGCG ACAAGATCAG CGACCAGGCC 
CTGACACACA TAGCCCAGGG ACTCTACAGA CTGAGATCCC TGTCCCTCAA TCAGTGCCAG 
ATCACCGATC ACGGCATGCT AAAGATCGCC AAGGCATTGC ACGAGTTGGA GAACCTCAAT 
ATCGGACAGT GCAGCCGGAT AACGGACAAG GGCCTGCAAA CGCTGGCCGA GGATCTGACT 
AATCTCAAGA CCATCGATCT CTACGGCTGC ACGCAACTCA GTTCCAAGGG CATCGACATC 
ATCATGAAGC TGCCCAAGCT GCAGAAGCTC AACCTGGGCC TCTGGCTGGT CAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppa ( NM_001299814.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286743.1 Drosophila melanogaster partner of paired, transcript variant C (Ppa), mRNA.

Price & Availability↑

CloneID	OFa12567
Clone ID Related Accession (Same CDS sequence)	NM_079088.3 , NM_001299814.1
Accession Version	NM_001299814.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	partner of paired, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGACCA ACACGGAGCT GCTGCCCTTC CATCATCAAA CAATCGCGGT GTTGAACAAT 
CTGAATAGCC ACTGTGGCGG ATCCGGGAGC AACGGAGCGG CCACCAGTGG CGGCGCGGCG 
ATTGGCGGAG CAGGAGCCGG AGGAGCGCCC AGCTGGGCCA TGGACGAACT CTATCAACAG 
ACTGAGCTGC CCGGTCTGAC GCGAACCGGA GCCACCACCA CCCACCACCA CCTGCTGCGC 
TTCACGCCCT ACGCCCTGCA CCACCGACCG CCGCACCACC AGCTGCAGAC CTTGCCGCCG 
GCCCTCTACT TGCAGCATGC CGCAGCAGCT GCGGCGGCAG CGGCAGCAGC AGCGGCCCAC 
GCCCAGCACC ATCATCACCA TCACCACCAT CTCCCGCACA GACCAGCATC TCCGGAGAGC 
CCGCCGCCGG TGGAGGGCAC CCACATAAGC AACCTGTTCC CAGAACTCCT CGAGCAGATA 
TTCGAGCACC TACCAGTGAG GGATTTGGGT CGGGCAGCTC AGGTTTGCAC CGCATGGCGG 
GATGCGGCCT ATGCCAAGAG CGTTTGGAAG GGAGTCGAGG CCAAGCTGCA CCTTAAGCGT 
TCCAGTCCGA GCCTGTTCAA CTGCCTGGTC AAGCGCGGCA TCAAGAAGGT GCAGATCCTA 
TCGCTGCGCC GCTCGCTTAA GGATTTGGTC CTGGGCGTTC CGGCTCTGAC CTCTCTGAAC 
CTGAGCGGCT GCTTCAACGT GGCGGACATG AACCTGGGCC ATGCCTTTAG CGTGGATCTG 
CCAAACCTCA AGACACTGGA CCTCTCCCTC TGTAAACAGA TCACGGACAC GAGTCTGGGG 
CGCATCGCCC AGCATCTAAG GAATCTTGAG ACCCTGGAAC TGGGCGGCTG TTGCAACATC 
ACGAACACAG GACTGCTGCT CATTGCGTGG GGCCTGAAGA AGCTGAAGCA CCTCAATCTG 
CGCTCCTGCT GGCACATCAG CGACCAGGGA ATCGGACACC TGGCTGGTTT CTCGCGGGAA 
ACGGCCGAAG GCAACCTGCA GCTGGAGTAC TTGGGTCTGC AGGATTGCCA GCGGCTAAGC 
GACGAGGCTT TGGGCCACAT TGCCCAGGGC CTGACCTCGC TGAAGTCGAT CAACCTGAGC 
TTCTGCGTCT CGGTGACGGA CAGCGGACTA AAGCACCTGG CCCGGATGCC CAAGCTCGAG 
CAGCTGAACT TGCGCTCCTG CGACAACATC TCGGATATCG GAATGGCCTA CCTCACGGAA 
GGCGGCAGCG GGATCAACTC GCTGGACGTC AGTTTCTGCG ACAAGATCAG CGACCAGGCC 
CTGACACACA TAGCCCAGGG ACTCTACAGA CTGAGATCCC TGTCCCTCAA TCAGTGCCAG 
ATCACCGATC ACGGCATGCT AAAGATCGCC AAGGCATTGC ACGAGTTGGA GAACCTCAAT 
ATCGGACAGT GCAGCCGGAT AACGGACAAG GGCCTGCAAA CGCTGGCCGA GGATCTGACT 
AATCTCAAGA CCATCGATCT CTACGGCTGC ACGCAACTCA GTTCCAAGGG CATCGACATC 
ATCATGAAGC TGCCCAAGCT GCAGAAGCTC AACCTGGGCC TCTGGCTGGT CAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286743.1
CDS	267..1883
Translation	MATNTELLPFHHQTIAVLNNLNSHCGGSGSNGAATSGGAAIGGAGAGGAPSWAMDELYQQTELPGLTRTGATTTHHHLLRFTPYALHHRPPHHQLQTLPPALYLQHAAAAAAAAAAAAAHAQHHHHHHHHLPHRPASPESPPPVEGTHISNLFPELLEQIFEHLPVRDLGRAAQVCTAWRDAAYAKSVWKGVEAKLHLKRSSPSLFNCLVKRGIKKVQILSLRRSLKDLVLGVPALTSLNLSGCFNVADMNLGHAFSVDLPNLKTLDLSLCKQITDTSLGRIAQHLRNLETLELGGCCNITNTGLLLIAWGLKKLKHLNLRSCWHISDQGIGHLAGFSRETAEGNLQLEYLGLQDCQRLSDEALGHIAQGLTSLKSINLSFCVSVTDSGLKHLARMPKLEQLNLRSCDNISDIGMAYLTEGGSGINSLDVSFCDKISDQALTHIAQGLYRLRSLSLNQCQITDHGMLKIAKALHELENLNIGQCSRITDKGLQTLAEDLTNLKTIDLYGCTQLSSKGIDIIMKLPKLQKLNLGLWLVR

Target ORF information:

RefSeq Version	NM_001299814.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster partner of paired, transcript variant C (Ppa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299814.1

ORF Insert Sequence:

ATGGCGACCA ACACGGAGCT GCTGCCCTTC CATCATCAAA CAATCGCGGT GTTGAACAAT 
CTGAATAGCC ACTGTGGCGG ATCCGGGAGC AACGGAGCGG CCACCAGTGG CGGCGCGGCG 
ATTGGCGGAG CAGGAGCCGG AGGAGCGCCC AGCTGGGCCA TGGACGAACT CTATCAACAG 
ACTGAGCTGC CCGGTCTGAC GCGAACCGGA GCCACCACCA CCCACCACCA CCTGCTGCGC 
TTCACGCCCT ACGCCCTGCA CCACCGACCG CCGCACCACC AGCTGCAGAC CTTGCCGCCG 
GCCCTCTACT TGCAGCATGC CGCAGCAGCT GCGGCGGCAG CGGCAGCAGC AGCGGCCCAC 
GCCCAGCACC ATCATCACCA TCACCACCAT CTCCCGCACA GACCAGCATC TCCGGAGAGC 
CCGCCGCCGG TGGAGGGCAC CCACATAAGC AACCTGTTCC CAGAACTCCT CGAGCAGATA 
TTCGAGCACC TACCAGTGAG GGATTTGGGT CGGGCAGCTC AGGTTTGCAC CGCATGGCGG 
GATGCGGCCT ATGCCAAGAG CGTTTGGAAG GGAGTCGAGG CCAAGCTGCA CCTTAAGCGT 
TCCAGTCCGA GCCTGTTCAA CTGCCTGGTC AAGCGCGGCA TCAAGAAGGT GCAGATCCTA 
TCGCTGCGCC GCTCGCTTAA GGATTTGGTC CTGGGCGTTC CGGCTCTGAC CTCTCTGAAC 
CTGAGCGGCT GCTTCAACGT GGCGGACATG AACCTGGGCC ATGCCTTTAG CGTGGATCTG 
CCAAACCTCA AGACACTGGA CCTCTCCCTC TGTAAACAGA TCACGGACAC GAGTCTGGGG 
CGCATCGCCC AGCATCTAAG GAATCTTGAG ACCCTGGAAC TGGGCGGCTG TTGCAACATC 
ACGAACACAG GACTGCTGCT CATTGCGTGG GGCCTGAAGA AGCTGAAGCA CCTCAATCTG 
CGCTCCTGCT GGCACATCAG CGACCAGGGA ATCGGACACC TGGCTGGTTT CTCGCGGGAA 
ACGGCCGAAG GCAACCTGCA GCTGGAGTAC TTGGGTCTGC AGGATTGCCA GCGGCTAAGC 
GACGAGGCTT TGGGCCACAT TGCCCAGGGC CTGACCTCGC TGAAGTCGAT CAACCTGAGC 
TTCTGCGTCT CGGTGACGGA CAGCGGACTA AAGCACCTGG CCCGGATGCC CAAGCTCGAG 
CAGCTGAACT TGCGCTCCTG CGACAACATC TCGGATATCG GAATGGCCTA CCTCACGGAA 
GGCGGCAGCG GGATCAACTC GCTGGACGTC AGTTTCTGCG ACAAGATCAG CGACCAGGCC 
CTGACACACA TAGCCCAGGG ACTCTACAGA CTGAGATCCC TGTCCCTCAA TCAGTGCCAG 
ATCACCGATC ACGGCATGCT AAAGATCGCC AAGGCATTGC ACGAGTTGGA GAACCTCAAT 
ATCGGACAGT GCAGCCGGAT AACGGACAAG GGCCTGCAAA CGCTGGCCGA GGATCTGACT 
AATCTCAAGA CCATCGATCT CTACGGCTGC ACGCAACTCA GTTCCAAGGG CATCGACATC 
ATCATGAAGC TGCCCAAGCT GCAGAAGCTC AACCTGGGCC TCTGGCTGGT CAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ppa cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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