dnr1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	dnr1
Entrez Gene ID	37575
Full Name	defense repressor 1
Synonyms	CG12489,DNR1,Dmel\CG12489,Dnr1
General protein information	Preferred Names defense repressor 1 Names CG12489 gene product from transcript CG12489-RB CG12489-PA CG12489-PB defense repressor I dnr1-PA dnr1-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 58E9-58F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_137836.3	NP_611680.2	defense repressor 1, isoform A
NM_001274208.2	NP_001261137.1	defense repressor 1, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	MYLIP	NP_037394.2
Bos taurus (cattle)	MYLIP	NP_001095723.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001609	XP_551203.4
Danio rerio (zebrafish)	mylipa	NP_956277.1
Xenopus tropicalis (tropical clawed frog)	mylip	NP_001017059.2
Mus musculus (house mouse)	Mylip	NP_722484.2
Gallus gallus (chicken)	MYLIP	NP_001012579.1
Drosophila melanogaster (fruit fly)	dnr1	NP_611680.2
Canis lupus familiaris (dog)	MYLIP	XP_545352.3
Pan troglodytes (chimpanzee)	MYLIP	XP_518252.2
	MYLIP	XP_001094408.1
Rattus norvegicus (Norway rat)	Mylip	NP_001100814.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Dnr1 is a negative regulator of the immune deficiency innate immune-response pathway. Dnr1 mutants exhibit shortened lifespan and progressive, age-dependent neuropathology associated with activation of the Imd pathway and elevated expression of AMP genes.
Title: Dnr1 mutations cause neurodegeneration in Drosophila by activating the innate immune response in the brain.

Dnr1 is a negative regulator of the Imd pathway
Title: Dnr1-dependent regulation of the Drosophila immune deficiency signaling pathway.

A study was made of the interactions of Dnr1 with the apoptotic machinery.
Title: Interactions of DNR1 with the apoptotic machinery of Drosophila melanogaster.

The following dnr1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dnr1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10943	NM_137836.3 Latest version!	Drosophila melanogaster defense repressor 1 (dnr1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
OFa12283	NM_001274208.2 Latest version!	Drosophila melanogaster defense repressor 1 (dnr1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dnr1 ( NM_137836.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611680.2 Drosophila melanogaster defense repressor 1 (dnr1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa10943
Clone ID Related Accession (Same CDS sequence)	NM_137836.3
Accession Version	NM_137836.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	defense repressor 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137836.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGTGCA TTGTCAACCT GCCCAACGGC ACCCAACAAG CCGTCAAGTG GGATCCCAAA 
GCCAATGGTC AGGAGTGTCT GGAGAAGGTC TGTCGCGCAA TGAACATCAT CTGCGAGATG 
GAGTACTTCG GCCTGGAGCA CTGGACGCCC AACCAGAAGG AGTCGCAGAC GCGCCAGTGG 
ATCAATCTGC GCAATCGGCT ATCCGGCGAC AGTGGCAGCA GCGGCAGCGG CATCCAGCTG 
ATGCTGGCGC TGCGGGTCAA GTTCTGGGTG CCGGTGCACT TCATCCTGCA GGAGAGCGTG 
CGTAATTTGT TCTACATGCA GGCCAGGCGG GATCTGCTCG AGGGACGGCT CACTGCGCCG 
GACTGGAGTG GAGCGGCCAA GTTGGCGGCG CTGCTCTGCC AGGCGGATGG TCTGCGATTC 
AATGAGTCGT CGCTGAAGGC CGACTGCCCG ATGCGAATGA GGCGGGAGTT GGCGCAACAG 
CAGCAGCAGC AACAGCAGCA GGCGCAGCAG CATAGGTTGG AGCAGCAGCG CAAGGAGAAG 
GAGCATGTGC TGAGCTTCAA GAAGCGCCGC CTGTCCAAGC AGAAGTCCAT GGAGCACATC 
GAGGTCTGTA CGCTGCCAGC GGCAAGCACC ACCAGCTGCA GTCTCCAGCC CTCGCCATCC 
GCATCCGCCT CAGCATCTGC ATCCGCCTCA GCATCTGCAT CCGCCTCCAC ATCCGCCTGC 
TCGCAAACCC ACTCGAACAG CAGCTCGAGC AGCCCCAGCA ACAGCAGCAG CCAGACGGGC 
CTGGACGAGC GACTGGCCAG CAATCCGCTC CGCGTCTACG AGGAGTACTT CATGCAGCCG 
AGCTGCGAGG GTGAGCCACC GGCGGACTAT CTGCGCCAAA TAGCCGTGGA GCACGGCAAG 
CTGGCCAAGC TGCAGATGAG CCTCAAGACG GCCAAGTACT GGCTGCTCAA GTCCATCCAG 
GATCTCGAAG GCTACGGCGA GGAGCTCTTT AGCGGCGTGA CCACCAACGA GAGTGCCACG 
CGCTGCGACA TTGCGGTGGG CGCCCATGGC ATCACCGTCT GCCGGGGGGG CGAGAAACAG 
AGCATCCCCT TTGGCGCCAT CGCTGCGGCC AAGTCGCTGC GTCGCACTTT CAAGTTGGAG 
TACGTGGATG ACCACAACGA TCGCAAGGAG CTGGAGATCA AGCTGCCCAA GCAGCCGATC 
GCCGCGGGCC TGTACCGCTC CATCACGGAG CGCCACGCCT TCTACGTGTG CGACAAGGTG 
CGCGGAGTGG TGACCAATCA GTTTACCCGG GATCTCAAGG GCACCATCGC CTCCATGTTC 
ATGGAGAATA CGGAGCTGGG CAAGCGCTAT GTCTTCGACA TTCAGCACAC GTGTCGCGAG 
GTGCACGACC AGGCGAGGAG GACTCTCCAC GAGCGGGGCG GCGATCTGGT GGCGGAGGGA 
GCCGAGGGAT GCTCTGCTGT CGCTGGAGGA TTGGGCGCCT CTGCCGTCGG CGAGCCGGGC 
GTCAGCCCAT GGGCCATGGC CCTGACGACT GGTGCCGGCG GCTCCATGGC CGGCAAGATA 
GATTTGGCCA TACGCGAGAA GGAGGCGCGT GAGGCGGCGA TTGAGCGTTG CGTGGACACG 
CGCATCTCGG AGGCCATGCA GTGCAAGATC TGCATGGACC GCGCCATCAA CACAGTGTTC 
AATCCGTGCT GTCACGTCAT CGCCTGCGCC CAGTGCGCTG CACGATGCAG CAACTGTCCC 
AACTGTCGGG TGAAGATCAC CAGCGTGGTC AAAATCTATC TGCCGCCGGA GCTGCGCACC 
AGCCAAACCG GCAGCGGCGC CACAACCACC AGCAGCAGCA GCATCATGGG CGACGGCCAG 
GTGGAGGAGC AGCTGCTCCA ACAGCAGCTG GACGAGATCT CGGCGGCACC CGCATCCCTC 
GAGGCGGGCG CAGATGCAGG AGTGGGAGTT GGCGGCATGG CAGTGGGAGC AGGAGCAGGA 
GCAGGAGCAA CCGGACCTGG CGGACAGCCG AAGGTTACGA CGGCCGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611680.2
CDS	555..2585
Translation	MWCIVNLPNGTQQAVKWDPKANGQECLEKVCRAMNIICEMEYFGLEHWTPNQKESQTRQWINLRNRLSGDSGSSGSGIQLMLALRVKFWVPVHFILQESVRNLFYMQARRDLLEGRLTAPDWSGAAKLAALLCQADGLRFNESSLKADCPMRMRRELAQQQQQQQQQAQQHRLEQQRKEKEHVLSFKKRRLSKQKSMEHIEVCTLPAASTTSCSLQPSPSASASASASASASASASTSACSQTHSNSSSSSPSNSSSQTGLDERLASNPLRVYEEYFMQPSCEGEPPADYLRQIAVEHGKLAKLQMSLKTAKYWLLKSIQDLEGYGEELFSGVTTNESATRCDIAVGAHGITVCRGGEKQSIPFGAIAAAKSLRRTFKLEYVDDHNDRKELEIKLPKQPIAAGLYRSITERHAFYVCDKVRGVVTNQFTRDLKGTIASMFMENTELGKRYVFDIQHTCREVHDQARRTLHERGGDLVAEGAEGCSAVAGGLGASAVGEPGVSPWAMALTTGAGGSMAGKIDLAIREKEAREAAIERCVDTRISEAMQCKICMDRAINTVFNPCCHVIACAQCAARCSNCPNCRVKITSVVKIYLPPELRTSQTGSGATTTSSSSIMGDGQVEEQLLQQQLDEISAAPASLEAGADAGVGVGGMAVGAGAGAGATGPGGQPKVTTAA

Target ORF information:

RefSeq Version	NM_137836.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster defense repressor 1 (dnr1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137836.3

ORF Insert Sequence:

ATGTGGTGCA TTGTCAACCT GCCCAACGGC ACCCAACAAG CCGTCAAGTG GGATCCCAAA 
GCCAATGGTC AGGAGTGTCT GGAGAAGGTC TGTCGCGCAA TGAACATCAT CTGCGAGATG 
GAGTACTTCG GCCTGGAGCA CTGGACGCCC AACCAGAAGG AGTCGCAGAC GCGCCAGTGG 
ATCAATCTGC GCAATCGGCT ATCCGGCGAC AGTGGCAGCA GCGGCAGCGG CATCCAGCTG 
ATGCTGGCGC TGCGGGTCAA GTTCTGGGTG CCGGTGCACT TCATCCTGCA GGAGAGCGTG 
CGTAATTTGT TCTACATGCA GGCCAGGCGG GATCTGCTCG AGGGACGGCT CACTGCGCCG 
GACTGGAGTG GAGCGGCCAA GTTGGCGGCG CTGCTCTGCC AGGCGGATGG TCTGCGATTC 
AATGAGTCGT CGCTGAAGGC CGACTGCCCG ATGCGAATGA GGCGGGAGTT GGCGCAACAG 
CAGCAGCAGC AACAGCAGCA GGCGCAGCAG CATAGGTTGG AGCAGCAGCG CAAGGAGAAG 
GAGCATGTGC TGAGCTTCAA GAAGCGCCGC CTGTCCAAGC AGAAGTCCAT GGAGCACATC 
GAGGTCTGTA CGCTGCCAGC GGCAAGCACC ACCAGCTGCA GTCTCCAGCC CTCGCCATCC 
GCATCCGCCT CAGCATCTGC ATCCGCCTCA GCATCTGCAT CCGCCTCCAC ATCCGCCTGC 
TCGCAAACCC ACTCGAACAG CAGCTCGAGC AGCCCCAGCA ACAGCAGCAG CCAGACGGGC 
CTGGACGAGC GACTGGCCAG CAATCCGCTC CGCGTCTACG AGGAGTACTT CATGCAGCCG 
AGCTGCGAGG GTGAGCCACC GGCGGACTAT CTGCGCCAAA TAGCCGTGGA GCACGGCAAG 
CTGGCCAAGC TGCAGATGAG CCTCAAGACG GCCAAGTACT GGCTGCTCAA GTCCATCCAG 
GATCTCGAAG GCTACGGCGA GGAGCTCTTT AGCGGCGTGA CCACCAACGA GAGTGCCACG 
CGCTGCGACA TTGCGGTGGG CGCCCATGGC ATCACCGTCT GCCGGGGGGG CGAGAAACAG 
AGCATCCCCT TTGGCGCCAT CGCTGCGGCC AAGTCGCTGC GTCGCACTTT CAAGTTGGAG 
TACGTGGATG ACCACAACGA TCGCAAGGAG CTGGAGATCA AGCTGCCCAA GCAGCCGATC 
GCCGCGGGCC TGTACCGCTC CATCACGGAG CGCCACGCCT TCTACGTGTG CGACAAGGTG 
CGCGGAGTGG TGACCAATCA GTTTACCCGG GATCTCAAGG GCACCATCGC CTCCATGTTC 
ATGGAGAATA CGGAGCTGGG CAAGCGCTAT GTCTTCGACA TTCAGCACAC GTGTCGCGAG 
GTGCACGACC AGGCGAGGAG GACTCTCCAC GAGCGGGGCG GCGATCTGGT GGCGGAGGGA 
GCCGAGGGAT GCTCTGCTGT CGCTGGAGGA TTGGGCGCCT CTGCCGTCGG CGAGCCGGGC 
GTCAGCCCAT GGGCCATGGC CCTGACGACT GGTGCCGGCG GCTCCATGGC CGGCAAGATA 
GATTTGGCCA TACGCGAGAA GGAGGCGCGT GAGGCGGCGA TTGAGCGTTG CGTGGACACG 
CGCATCTCGG AGGCCATGCA GTGCAAGATC TGCATGGACC GCGCCATCAA CACAGTGTTC 
AATCCGTGCT GTCACGTCAT CGCCTGCGCC CAGTGCGCTG CACGATGCAG CAACTGTCCC 
AACTGTCGGG TGAAGATCAC CAGCGTGGTC AAAATCTATC TGCCGCCGGA GCTGCGCACC 
AGCCAAACCG GCAGCGGCGC CACAACCACC AGCAGCAGCA GCATCATGGG CGACGGCCAG 
GTGGAGGAGC AGCTGCTCCA ACAGCAGCTG GACGAGATCT CGGCGGCACC CGCATCCCTC 
GAGGCGGGCG CAGATGCAGG AGTGGGAGTT GGCGGCATGG CAGTGGGAGC AGGAGCAGGA 
GCAGGAGCAA CCGGACCTGG CGGACAGCCG AAGGTTACGA CGGCCGCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dnr1 ( NM_001274208.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261137.1 Drosophila melanogaster defense repressor 1 (dnr1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa12283
Clone ID Related Accession (Same CDS sequence)	NM_001274208.2
Accession Version	NM_001274208.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	defense repressor 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001274208.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGTGCA TTGTCAACCT GCCCAACGGC ACCCAACAAG CCGTCAAGTG GGATCCCAAA 
GCCAATGGTC AGGAGTGTCT GGAGAAGGTC TGTCGCGCAA TGAACATCAT CTGCGAGATG 
GAGTACTTCG GCCTGGAGCA CTGGACGCCC AACCAGAAGG AGTCGCAGAC GCGCCAGTGG 
ATCAATCTGC GCAATCGGCT ATCCGGCGAC AGTGGCAGCA GCGGCAGCGG CATCCAGCTG 
ATGCTGGCGC TGCGGGTCAA GTTCTGGGTG CCGGTGCACT TCATCCTGCA GGAGAGCGTG 
CGTAATTTGT TCTACATGCA GGCCAGGCGG GATCTGCTCG AGGGACGGCT CACTGCGCCG 
GACTGGAGTG GAGCGGCCAA GTTGGCGGCG CTGCTCTGCC AGGCGGATGG TCTGCGATTC 
AATGAGTCGT CGCTGAAGGC CGACTGCCCG ATGCGAATGA GGCGGGAGTT GGCGCAACAG 
CAGCAGCAGC AACAGCAGCA GGCGCAGCAG CATAGGTTGG AGCAGCAGCG CAAGGAGAAG 
GAGCATGTGC TGAGCTTCAA GAAGCGCCGC CTGTCCAAGC AGAAGTCCAT GGAGCACATC 
GAGGTCTGTA CGCTGCCAGC GGCAAGCACC ACCAGCTGCA GTCTCCAGCC CTCGCCATCC 
GCATCCGCCT CAGCATCTGC ATCCGCCTCA GCATCTGCAT CCGCCTCCAC ATCCGCCTGC 
TCGCAAACCC ACTCGAACAG CAGCTCGAGC AGCCCCAGCA ACAGCAGCAG CCAGACGGGC 
CTGGACGAGC GACTGGCCAG CAATCCGCTC CGCGTCTACG AGGAGTACTT CATGCAGCCG 
AGCTGCGAGG GTGAGCCACC GGCGGACTAT CTGCGCCAAA TAGCCGTGGA GCACGGCAAG 
CTGGCCAAGC TGCAGATGAG CCTCAAGACG GCCAAGTACT GGCTGCTCAA GTCCATCCAG 
GATCTCGAAG GCTACGGCGA GGAGCTCTTT AGCGGCGTGA CCACCAACGA GAGTGCCACG 
CGCTGCGACA TTGCGGTGGG CGCCCATGGC ATCACCGTCT GCCGGGGGGG CGAGAAACAG 
AGCATCCCCT TTGGCGCCAT CGCTGCGGCC AAGTCGCTGC GTCGCACTTT CAAGTTGGAG 
TACGTGGATG ACCACAACGA TCGCAAGGAG CTGGAGATCA AGCTGCCCAA GCAGCCGATC 
GCCGCGGGCC TGTACCGCTC CATCACGGAG CGCCACGCCT TCTACGTGTG CGACAAGGTG 
CGCGGAGTGG TGACCAATCA GTTTACCCGG GATCTCAAGG GCACCATCGC CTCCATGTTC 
ATGGAGAATA CGGAGCTGGG CAAGCGCTAT GTCTTCGACA TTCAGCACAC GTGTCGCGAG 
GTGCACGACC AGGCGAGGAG GACTCTCCAC GAGCGGGGCG GCGATCTGGT GGCGGAGGGA 
GCCGAGGGAT GCTCTGCTGT CGCTGGAGGA TTGGGCGCCT CTGCCGTCGG CGAGCCGGGC 
GTCAGCCCAT GGGCCATGGC CCTGACGACT GGTGCCGGCG GCTCCATGGC CGGCAAGATA 
GATTTGGCCA TACGCGAGAA GGAGGCGCGT GAGGCGGCGA TTGAGCGTTG CGTGGACACG 
CGCATCTCGG AGGCCATGCA GTGCAAGATC TGCATGGACC GCGCCATCAA CACAGTGTTC 
AATCCGTGCT GTCACGTCAT CGCCTGCGCC CAGTGCGCTG CACGATGCAG CAACTGTCCC 
AACTGTCGGG TGAAGATCAC CAGCGTGGTC AAAATCTATC TGCCGCCGGA GCTGCGCACC 
AGCCAAACCG GCAGCGGCGC CACAACCACC AGCAGCAGCA GCATCATGGG CGACGGCCAG 
GTGGAGGAGC AGCTGCTCCA ACAGCAGCTG GACGAGATCT CGGCGGCACC CGCATCCCTC 
GAGGCGGGCG CAGATGCAGG AGTGGGAGTT GGCGGCATGG CAGTGGGAGC AGGAGCAGGA 
GCAGGAGCAA CCGGACCTGG CGGACAGCCG AAGGTTACGA CGGCCGCCTA GAATTGGTTG 
TGCTCGCTGC TGTTCCGGCT GATGGAATGG GTGTGCGTGC CGATTGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261137.1
CDS	555..2645
Translation	MWCIVNLPNGTQQAVKWDPKANGQECLEKVCRAMNIICEMEYFGLEHWTPNQKESQTRQWINLRNRLSGDSGSSGSGIQLMLALRVKFWVPVHFILQESVRNLFYMQARRDLLEGRLTAPDWSGAAKLAALLCQADGLRFNESSLKADCPMRMRRELAQQQQQQQQQAQQHRLEQQRKEKEHVLSFKKRRLSKQKSMEHIEVCTLPAASTTSCSLQPSPSASASASASASASASASTSACSQTHSNSSSSSPSNSSSQTGLDERLASNPLRVYEEYFMQPSCEGEPPADYLRQIAVEHGKLAKLQMSLKTAKYWLLKSIQDLEGYGEELFSGVTTNESATRCDIAVGAHGITVCRGGEKQSIPFGAIAAAKSLRRTFKLEYVDDHNDRKELEIKLPKQPIAAGLYRSITERHAFYVCDKVRGVVTNQFTRDLKGTIASMFMENTELGKRYVFDIQHTCREVHDQARRTLHERGGDLVAEGAEGCSAVAGGLGASAVGEPGVSPWAMALTTGAGGSMAGKIDLAIREKEAREAAIERCVDTRISEAMQCKICMDRAINTVFNPCCHVIACAQCAARCSNCPNCRVKITSVVKIYLPPELRTSQTGSGATTTSSSSIMGDGQVEEQLLQQQLDEISAAPASLEAGADAGVGVGGMAVGAGAGAGATGPGGQPKVTTAA*NWLCSLLFRLMEWVCVPIV

Target ORF information:

RefSeq Version	NM_001274208.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster defense repressor 1 (dnr1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274208.2

ORF Insert Sequence:

ATGTGGTGCA TTGTCAACCT GCCCAACGGC ACCCAACAAG CCGTCAAGTG GGATCCCAAA 
GCCAATGGTC AGGAGTGTCT GGAGAAGGTC TGTCGCGCAA TGAACATCAT CTGCGAGATG 
GAGTACTTCG GCCTGGAGCA CTGGACGCCC AACCAGAAGG AGTCGCAGAC GCGCCAGTGG 
ATCAATCTGC GCAATCGGCT ATCCGGCGAC AGTGGCAGCA GCGGCAGCGG CATCCAGCTG 
ATGCTGGCGC TGCGGGTCAA GTTCTGGGTG CCGGTGCACT TCATCCTGCA GGAGAGCGTG 
CGTAATTTGT TCTACATGCA GGCCAGGCGG GATCTGCTCG AGGGACGGCT CACTGCGCCG 
GACTGGAGTG GAGCGGCCAA GTTGGCGGCG CTGCTCTGCC AGGCGGATGG TCTGCGATTC 
AATGAGTCGT CGCTGAAGGC CGACTGCCCG ATGCGAATGA GGCGGGAGTT GGCGCAACAG 
CAGCAGCAGC AACAGCAGCA GGCGCAGCAG CATAGGTTGG AGCAGCAGCG CAAGGAGAAG 
GAGCATGTGC TGAGCTTCAA GAAGCGCCGC CTGTCCAAGC AGAAGTCCAT GGAGCACATC 
GAGGTCTGTA CGCTGCCAGC GGCAAGCACC ACCAGCTGCA GTCTCCAGCC CTCGCCATCC 
GCATCCGCCT CAGCATCTGC ATCCGCCTCA GCATCTGCAT CCGCCTCCAC ATCCGCCTGC 
TCGCAAACCC ACTCGAACAG CAGCTCGAGC AGCCCCAGCA ACAGCAGCAG CCAGACGGGC 
CTGGACGAGC GACTGGCCAG CAATCCGCTC CGCGTCTACG AGGAGTACTT CATGCAGCCG 
AGCTGCGAGG GTGAGCCACC GGCGGACTAT CTGCGCCAAA TAGCCGTGGA GCACGGCAAG 
CTGGCCAAGC TGCAGATGAG CCTCAAGACG GCCAAGTACT GGCTGCTCAA GTCCATCCAG 
GATCTCGAAG GCTACGGCGA GGAGCTCTTT AGCGGCGTGA CCACCAACGA GAGTGCCACG 
CGCTGCGACA TTGCGGTGGG CGCCCATGGC ATCACCGTCT GCCGGGGGGG CGAGAAACAG 
AGCATCCCCT TTGGCGCCAT CGCTGCGGCC AAGTCGCTGC GTCGCACTTT CAAGTTGGAG 
TACGTGGATG ACCACAACGA TCGCAAGGAG CTGGAGATCA AGCTGCCCAA GCAGCCGATC 
GCCGCGGGCC TGTACCGCTC CATCACGGAG CGCCACGCCT TCTACGTGTG CGACAAGGTG 
CGCGGAGTGG TGACCAATCA GTTTACCCGG GATCTCAAGG GCACCATCGC CTCCATGTTC 
ATGGAGAATA CGGAGCTGGG CAAGCGCTAT GTCTTCGACA TTCAGCACAC GTGTCGCGAG 
GTGCACGACC AGGCGAGGAG GACTCTCCAC GAGCGGGGCG GCGATCTGGT GGCGGAGGGA 
GCCGAGGGAT GCTCTGCTGT CGCTGGAGGA TTGGGCGCCT CTGCCGTCGG CGAGCCGGGC 
GTCAGCCCAT GGGCCATGGC CCTGACGACT GGTGCCGGCG GCTCCATGGC CGGCAAGATA 
GATTTGGCCA TACGCGAGAA GGAGGCGCGT GAGGCGGCGA TTGAGCGTTG CGTGGACACG 
CGCATCTCGG AGGCCATGCA GTGCAAGATC TGCATGGACC GCGCCATCAA CACAGTGTTC 
AATCCGTGCT GTCACGTCAT CGCCTGCGCC CAGTGCGCTG CACGATGCAG CAACTGTCCC 
AACTGTCGGG TGAAGATCAC CAGCGTGGTC AAAATCTATC TGCCGCCGGA GCTGCGCACC 
AGCCAAACCG GCAGCGGCGC CACAACCACC AGCAGCAGCA GCATCATGGG CGACGGCCAG 
GTGGAGGAGC AGCTGCTCCA ACAGCAGCTG GACGAGATCT CGGCGGCACC CGCATCCCTC 
GAGGCGGGCG CAGATGCAGG AGTGGGAGTT GGCGGCATGG CAGTGGGAGC AGGAGCAGGA 
GCAGGAGCAA CCGGACCTGG CGGACAGCCG AAGGTTACGA CGGCCGCCTA GAATTGGTTG 
TGCTCGCTGC TGTTCCGGCT GATGGAATGG GTGTGCGTGC CGATTGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
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The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
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dnr1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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