rad50 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	rad50
Entrez Gene ID	37564
Full Name	CG6339 gene product from transcript CG6339-RE
Synonyms	6339,CG6339,Dmel\CG6339,Dmrad50,RAD50,Rad50
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 58E1-58E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_166533.4	NP_726199.3	rad50, isoform D
NM_001259532.2	NP_001246461.1	rad50, isoform E

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os02g0497500	NP_001046893.1
Xenopus tropicalis (tropical clawed frog)	rad50	XP_004912891.1
	RAD50	XP_001099384.2
Gallus gallus (chicken)	RAD50	XP_004945072.1
Arabidopsis thaliana (thale cress)	RAD50	NP_565733.1
Homo sapiens (human)	RAD50	NP_005723.2
Rattus norvegicus (Norway rat)	Rad50	NP_071582.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003676	XP_313457.4
Pan troglodytes (chimpanzee)	RAD50	XP_001163412.1
Canis lupus familiaris (dog)	RAD50	XP_531901.2
Neurospora crassa	NCU00901	XP_963818.1
Bos taurus (cattle)	RAD50	NP_001193797.1
Mus musculus (house mouse)	Rad50	NP_033038.2
Danio rerio (zebrafish)	rad50	XP_005167995.1
Drosophila melanogaster (fruit fly)	rad50	NP_001246461.1
Magnaporthe oryzae (rice blast fungus)	MGG_11387	XP_003713570.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study identified a stringent requirement for Mre11-Rad50-Nbs (MRN) function in telomere protection during early embryonic development.
Title: Mre11-Rad50-Nbs complex is required to cap telomeres during Drosophila embryogenesis.

The following rad50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rad50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10930	NM_166533.4 Latest version!	Drosophila melanogaster rad50, transcript variant D (rad50), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa12278	NM_001259532.2 Latest version!	Drosophila melanogaster rad50, transcript variant E (rad50), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rad50 ( NM_166533.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726199.3 Drosophila melanogaster rad50, transcript variant D (rad50), mRNA.

Price & Availability↑

CloneID	OFa10930
Clone ID Related Accession (Same CDS sequence)	NM_166533.4
Accession Version	NM_166533.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rad50, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166533.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTCAA TTGAATCGCT GTCCATCCAG GGAATACGGA GTTTCGGCAC CTATGCCGAC 
GATCTTCAGA GTATAAAGTT TTCCTCGCCG GTTACCCTGA TTCTGGGCGA GAACGGATGC 
GGAAAGACGA CCGTGGTAGA GTGTCTCAAG TACGCCTTGA CCGGCGAGTG TCCGCCGGGC 
AGTGATCGGG GCAAGAGTTT CGTCCATGAC CCTAAGATCT TTGGGCTAAA CGAGGTGCTA 
GCGCAGATCA AGATGCAGGT GCGGGACAGG CGGGGTGCCC AAGTGTCCAT CTGCCGCACC 
ATGAAGGTGT CCAAGAAGCG CAACAAAATG TCCTTTGAAA CAATGGACTC CACCATCAAC 
TTCCTGACCG GCGCTGGACA GTCGAAGCGC GAAAAGCAGG ACTCGCTAAG CGGCCGCTCC 
GTGGATATCG ACGTGGCCAT CTCGGACTTC ATGGGTGTCT CCAAGGCTAT TATCAACAAT 
GTCCTGTTTT GCCATCAAGA GGACTCCAGT TGGCCGCTGG ACGAATCAAA AAAGTTAAAG 
GAAAAGTTCG ATGCCATATT CGGCATCACC GAGTACAATA AGGCGCTGGA TAAAATCATA 
AAGCTTCGCA AGGAGGCTAT GGAAGAGCTA AAAATAAAGG AGGCCAACAT AAAGCATGTG 
GCCTACCTCA AGCAGGAAAT GGAGGTTAAA ACTTTGAACC TACAAAAGGC GCAGAGGAAA 
TGCGACGCAA TTAAGGCGCA GTGCAGTGAG TGCGAGGAGG AAATGAAGCC GATTGAGGCC 
AGGCTGGTGG AAATCCGAAA TGTAGAGTTC GAAATTGGGA AGTATCAAGC GCAGAAAGTC 
GAAATGGACA CCAAGCACAA AAACTGCAAG GATCAGATAT CCACTCTAAC CCTGAAGATC 
AAGAAACCCT TTAGAGGTAC CCTGGATGAA TTGGATCAAG AGATAAGTAA CTTTGATCAG 
CGCATGCTAG AGATGCGTCA GAAACGCACC GAAGTGGAGG GGGATTTGTC GCAGATTAAG 
AGGTCCAGTG TCGCTGAGCA AGAAAAGTTG GGCACACAGG ACCGGAAGCA TTGCCTGGCT 
AAACAACGGC ATCAGAGCGA GCTGGCTTGT CGGGCACAGC TTCTGAAGCG GGTTAAGGAG 
TTCTGCCGGG AACTCCATAT ACCCATCGAT TGTGATCTTG TGGAGCAGCC TGAGAAAATG 
GGAGAAGTCC TTCGGGATAT CGAGGCGATG ATTATAACCA AGCACTGCGA AATCACAGAG 
ATAGTCGAGC AGAACGAGAA GGCCGATCGG AGCCGGCAAG TCAAGATCGA CGAGCTTCGC 
ATTGAGCTGA CCAAGTCGGA GCAAAGTGTA ACCGCGCAGG AGAAGCAGCG GGAGTCCAGC 
AAGCGAGAGA GTGAAACTCT TGGAGTGGAG ATAAAGAAAA TCGAAACATC CATGCAAGAC 
CTAAAGAAGC TTGAGAAAGA AATAAACGAG GTTAATGAGT TGTATGAGAG CGCTACGAAA 
AACATTGACC AACAGGCTAT AAAAGATGCC ATTGCCAGAA AGAAAGCCAG CATTGCCGAG 
AATCAGATTC AGTTTAAGAA GCTGGACGAA CAATTGACAT TCCTAGGCTC CATGGCCAAG 
CTAGTTGCCG AGTGTTCCCT GAAGCAAAAG GAGCTCGATA AAAAGAACCA AGAGGTACAT 
CGCGTGCGCA GCCGACACTC TGATCATTTC GGGAAGTTGT TCAAGGAACC AATTACCTGC 
AATTATCGCC GATCGATGCA GGTGGTCTAC GAAAAACTTC GTCGCGAGAT CCAAGAACTT 
AACGAGAAGG CAAACACTCA GAAACTAAAA GAACAGTCAT ACGAAATCAA GCGCAAAAAC 
CTCATTAGTG ATATTTCGCG AATGGAAAAG GAGTTAAAGG ACAGCGAGGA GTTAATATAT 
CAAAAGTGCC GGTCAACTCC GTATGATGAT CTGCTGGAGC GAAGTAAGAC GACTATTTCC 
AAATTGCAAT TCGACCATGG CGCCCTTAAA TCGTCCGAGG CCCTTTATAA GAAGTATATA 
CAGAAAATGG ATGAGGAGCC AAGTTGCCCA TTATGCCACC ACAACATGAC TAGTGATGAG 
GCGTGCGACT TGACTAGCGA GCTAACGGAT GAGATCCAAA AGTTGCCCGA TAATATCACT 
AGAGCTGAGA AAGCCTTAAA GGCGGAGCAG ATAAAATATG AGAACTTATT GCAACTTAAA 
CCCACCATTC TTAAGGTCAA GGAGCTTAAG GATTCGTTGC CACAAAAGAA GGAGGAACTG 
AAAAAGGTGG AGGAGCTGCT AGGAGACAGC GTCAGCGAAT ATGAAACATT AATTGCTCTT 
ATTGGGGAGC CAACTCATAA TATGGAGTTG GCGAATTCAA TGATGGGTGA TATGTCCCTA 
TTAGATGAGG CTTTAAAAGA TTCGGCGCGG CTCACAAAAG ATCTGGATCT GCAAAAGGGA 
CAGTTACCCG CTTCCTATGA TTCAAGTGTG TCTATGGATG ATCTGCAGGC GGAGAAGAGT 
AAGGTTTCAA AAGAGTTGGA GACGGAACGT AAGGAGTTGG AATCTGCTCA GAATGCAGTC 
CAGCAGCAAA TGGATGCCCT TAATCGTTTG CGCGAAAAGA AGAATAGTTT AAAAGACAGG 
CAAATACATC TTCGAGAGGG TCTTCAAAGC TTGCCACAGT TAAAGGAACG TTTGGAGAAG 
CTCAATAGTT TCCTTACAAC AGTAGCCTCT GAAATCAGCG AATTAAAGGC GAAAATCCAG 
CCTCTTAAGC TGAATTTACG AGCAGCCATA GAAGAAAAAG AGCGCTTGAA GAAAAGCGAA 
AGTGAAAAGT TAGCGCAGCT GAATTCCAAG TACAATTCCT ACAAAAGCAC AGATCACGAC 
ATACAAAGAT TGAACAAAGA AGCTGAGGAC TACGCCAAAT TGGATCTTCG AAATGAAATC 
AAAAAGCTTG ATGAAATCAT AATGGCCTCC AAAGATAAGT TAAGGAAATT GGAAGCCGAA 
ATATCTTTAA AAACTGATGA GCTGGAAACA ATTAAAACAG AGTGCTCCAA TCAGCAGACC 
GTTGAACGGG ATTTGAAGGA TAACCGTGAG CTAAAACAGC TCGAAGATAA AGAAGCCAAG 
CTAAGAGAAA GTTGCCAGGT GCTGGACAAG CAGCTGGGTA ACCTTGACTT TCATAGCGTT 
AGCAAGGAGA AAGTGAATCT AACTAAGCAA AGGGATAAGG CCACAGTGCG TAAGGGCGAA 
CTGCTGGGCC AGCTGGGCGA GATTCACAGC CAGGTGAACA AGTTGCAGCG TGAAATCGAC 
GAGCCCAGAT TTAAGGAGTC TTTGAAAAAC TTCCGGAAGG CCAATTACGA GATAGAGGTG 
ACACGCCTCT GCATTGAGGA CTTGGGCCAA TATCGTTTAG CGTTGGAGTG GGCCCTTATC 
CAGTTTCACT CTGAAAAAAT GGAGATGATT AACCGTCTGA TCCGCGAGTA CTGGCGTAAG 
ATATATCGCG GAAATGACAT TGACTATATT CAGGTGAAGA CTGATGAGGT AAGCTCGGAT 
GCTTCGGCGG ATCGGCGAAA GACATACAAC TACAGAGTGG TCCAGTCGAA GAACTACTCA 
GAGATCGAGA TGCGGGGACG CTGTAGTGCT GGCCAGCGCG TCCTTGCCTC CCTTATTATC 
CGCTTGGCAC TGGCCGAGAC CTTCAGCAGT AATTGTGGTG TCCTAGCTCT GGATGAGCCC 
ACCACGAACT TGGATCGCGC CAATATAAAC TCGCTATGTG AAGCCCTGAA TTGCATCGTG 
GAGGAGCGCC AAAGCCAGTC CAACTTTATG CTCATCATCA TCACTCACGA CGAGAATTTC 
GTTTCGTCTC TGGGCAAGAT AACCTCCTAC CATCGGGTCT TCCGCAACGA GGAGTGCAAG 
TCGGTTATAC GGAGAGTGGA GGCGGGTCCA TCAAAGAAAG CTTTGATAGA TCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726199.3
CDS	87..4043
Translation	MSSIESLSIQGIRSFGTYADDLQSIKFSSPVTLILGENGCGKTTVVECLKYALTGECPPGSDRGKSFVHDPKIFGLNEVLAQIKMQVRDRRGAQVSICRTMKVSKKRNKMSFETMDSTINFLTGAGQSKREKQDSLSGRSVDIDVAISDFMGVSKAIINNVLFCHQEDSSWPLDESKKLKEKFDAIFGITEYNKALDKIIKLRKEAMEELKIKEANIKHVAYLKQEMEVKTLNLQKAQRKCDAIKAQCSECEEEMKPIEARLVEIRNVEFEIGKYQAQKVEMDTKHKNCKDQISTLTLKIKKPFRGTLDELDQEISNFDQRMLEMRQKRTEVEGDLSQIKRSSVAEQEKLGTQDRKHCLAKQRHQSELACRAQLLKRVKEFCRELHIPIDCDLVEQPEKMGEVLRDIEAMIITKHCEITEIVEQNEKADRSRQVKIDELRIELTKSEQSVTAQEKQRESSKRESETLGVEIKKIETSMQDLKKLEKEINEVNELYESATKNIDQQAIKDAIARKKASIAENQIQFKKLDEQLTFLGSMAKLVAECSLKQKELDKKNQEVHRVRSRHSDHFGKLFKEPITCNYRRSMQVVYEKLRREIQELNEKANTQKLKEQSYEIKRKNLISDISRMEKELKDSEELIYQKCRSTPYDDLLERSKTTISKLQFDHGALKSSEALYKKYIQKMDEEPSCPLCHHNMTSDEACDLTSELTDEIQKLPDNITRAEKALKAEQIKYENLLQLKPTILKVKELKDSLPQKKEELKKVEELLGDSVSEYETLIALIGEPTHNMELANSMMGDMSLLDEALKDSARLTKDLDLQKGQLPASYDSSVSMDDLQAEKSKVSKELETERKELESAQNAVQQQMDALNRLREKKNSLKDRQIHLREGLQSLPQLKERLEKLNSFLTTVASEISELKAKIQPLKLNLRAAIEEKERLKKSESEKLAQLNSKYNSYKSTDHDIQRLNKEAEDYAKLDLRNEIKKLDEIIMASKDKLRKLEAEISLKTDELETIKTECSNQQTVERDLKDNRELKQLEDKEAKLRESCQVLDKQLGNLDFHSVSKEKVNLTKQRDKATVRKGELLGQLGEIHSQVNKLQREIDEPRFKESLKNFRKANYEIEVTRLCIEDLGQYRLALEWALIQFHSEKMEMINRLIREYWRKIYRGNDIDYIQVKTDEVSSDASADRRKTYNYRVVQSKNYSEIEMRGRCSAGQRVLASLIIRLALAETFSSNCGVLALDEPTTNLDRANINSLCEALNCIVEERQSQSNFMLIIITHDENFVSSLGKITSYHRVFRNEECKSVIRRVEAGPSKKALIDQ

Target ORF information:

RefSeq Version	NM_166533.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rad50, transcript variant D (rad50), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166533.4

ORF Insert Sequence:

ATGTCGTCAA TTGAATCGCT GTCCATCCAG GGAATACGGA GTTTCGGCAC CTATGCCGAC 
GATCTTCAGA GTATAAAGTT TTCCTCGCCG GTTACCCTGA TTCTGGGCGA GAACGGATGC 
GGAAAGACGA CCGTGGTAGA GTGTCTCAAG TACGCCTTGA CCGGCGAGTG TCCGCCGGGC 
AGTGATCGGG GCAAGAGTTT CGTCCATGAC CCTAAGATCT TTGGGCTAAA CGAGGTGCTA 
GCGCAGATCA AGATGCAGGT GCGGGACAGG CGGGGTGCCC AAGTGTCCAT CTGCCGCACC 
ATGAAGGTGT CCAAGAAGCG CAACAAAATG TCCTTTGAAA CAATGGACTC CACCATCAAC 
TTCCTGACCG GCGCTGGACA GTCGAAGCGC GAAAAGCAGG ACTCGCTAAG CGGCCGCTCC 
GTGGATATCG ACGTGGCCAT CTCGGACTTC ATGGGTGTCT CCAAGGCTAT TATCAACAAT 
GTCCTGTTTT GCCATCAAGA GGACTCCAGT TGGCCGCTGG ACGAATCAAA AAAGTTAAAG 
GAAAAGTTCG ATGCCATATT CGGCATCACC GAGTACAATA AGGCGCTGGA TAAAATCATA 
AAGCTTCGCA AGGAGGCTAT GGAAGAGCTA AAAATAAAGG AGGCCAACAT AAAGCATGTG 
GCCTACCTCA AGCAGGAAAT GGAGGTTAAA ACTTTGAACC TACAAAAGGC GCAGAGGAAA 
TGCGACGCAA TTAAGGCGCA GTGCAGTGAG TGCGAGGAGG AAATGAAGCC GATTGAGGCC 
AGGCTGGTGG AAATCCGAAA TGTAGAGTTC GAAATTGGGA AGTATCAAGC GCAGAAAGTC 
GAAATGGACA CCAAGCACAA AAACTGCAAG GATCAGATAT CCACTCTAAC CCTGAAGATC 
AAGAAACCCT TTAGAGGTAC CCTGGATGAA TTGGATCAAG AGATAAGTAA CTTTGATCAG 
CGCATGCTAG AGATGCGTCA GAAACGCACC GAAGTGGAGG GGGATTTGTC GCAGATTAAG 
AGGTCCAGTG TCGCTGAGCA AGAAAAGTTG GGCACACAGG ACCGGAAGCA TTGCCTGGCT 
AAACAACGGC ATCAGAGCGA GCTGGCTTGT CGGGCACAGC TTCTGAAGCG GGTTAAGGAG 
TTCTGCCGGG AACTCCATAT ACCCATCGAT TGTGATCTTG TGGAGCAGCC TGAGAAAATG 
GGAGAAGTCC TTCGGGATAT CGAGGCGATG ATTATAACCA AGCACTGCGA AATCACAGAG 
ATAGTCGAGC AGAACGAGAA GGCCGATCGG AGCCGGCAAG TCAAGATCGA CGAGCTTCGC 
ATTGAGCTGA CCAAGTCGGA GCAAAGTGTA ACCGCGCAGG AGAAGCAGCG GGAGTCCAGC 
AAGCGAGAGA GTGAAACTCT TGGAGTGGAG ATAAAGAAAA TCGAAACATC CATGCAAGAC 
CTAAAGAAGC TTGAGAAAGA AATAAACGAG GTTAATGAGT TGTATGAGAG CGCTACGAAA 
AACATTGACC AACAGGCTAT AAAAGATGCC ATTGCCAGAA AGAAAGCCAG CATTGCCGAG 
AATCAGATTC AGTTTAAGAA GCTGGACGAA CAATTGACAT TCCTAGGCTC CATGGCCAAG 
CTAGTTGCCG AGTGTTCCCT GAAGCAAAAG GAGCTCGATA AAAAGAACCA AGAGGTACAT 
CGCGTGCGCA GCCGACACTC TGATCATTTC GGGAAGTTGT TCAAGGAACC AATTACCTGC 
AATTATCGCC GATCGATGCA GGTGGTCTAC GAAAAACTTC GTCGCGAGAT CCAAGAACTT 
AACGAGAAGG CAAACACTCA GAAACTAAAA GAACAGTCAT ACGAAATCAA GCGCAAAAAC 
CTCATTAGTG ATATTTCGCG AATGGAAAAG GAGTTAAAGG ACAGCGAGGA GTTAATATAT 
CAAAAGTGCC GGTCAACTCC GTATGATGAT CTGCTGGAGC GAAGTAAGAC GACTATTTCC 
AAATTGCAAT TCGACCATGG CGCCCTTAAA TCGTCCGAGG CCCTTTATAA GAAGTATATA 
CAGAAAATGG ATGAGGAGCC AAGTTGCCCA TTATGCCACC ACAACATGAC TAGTGATGAG 
GCGTGCGACT TGACTAGCGA GCTAACGGAT GAGATCCAAA AGTTGCCCGA TAATATCACT 
AGAGCTGAGA AAGCCTTAAA GGCGGAGCAG ATAAAATATG AGAACTTATT GCAACTTAAA 
CCCACCATTC TTAAGGTCAA GGAGCTTAAG GATTCGTTGC CACAAAAGAA GGAGGAACTG 
AAAAAGGTGG AGGAGCTGCT AGGAGACAGC GTCAGCGAAT ATGAAACATT AATTGCTCTT 
ATTGGGGAGC CAACTCATAA TATGGAGTTG GCGAATTCAA TGATGGGTGA TATGTCCCTA 
TTAGATGAGG CTTTAAAAGA TTCGGCGCGG CTCACAAAAG ATCTGGATCT GCAAAAGGGA 
CAGTTACCCG CTTCCTATGA TTCAAGTGTG TCTATGGATG ATCTGCAGGC GGAGAAGAGT 
AAGGTTTCAA AAGAGTTGGA GACGGAACGT AAGGAGTTGG AATCTGCTCA GAATGCAGTC 
CAGCAGCAAA TGGATGCCCT TAATCGTTTG CGCGAAAAGA AGAATAGTTT AAAAGACAGG 
CAAATACATC TTCGAGAGGG TCTTCAAAGC TTGCCACAGT TAAAGGAACG TTTGGAGAAG 
CTCAATAGTT TCCTTACAAC AGTAGCCTCT GAAATCAGCG AATTAAAGGC GAAAATCCAG 
CCTCTTAAGC TGAATTTACG AGCAGCCATA GAAGAAAAAG AGCGCTTGAA GAAAAGCGAA 
AGTGAAAAGT TAGCGCAGCT GAATTCCAAG TACAATTCCT ACAAAAGCAC AGATCACGAC 
ATACAAAGAT TGAACAAAGA AGCTGAGGAC TACGCCAAAT TGGATCTTCG AAATGAAATC 
AAAAAGCTTG ATGAAATCAT AATGGCCTCC AAAGATAAGT TAAGGAAATT GGAAGCCGAA 
ATATCTTTAA AAACTGATGA GCTGGAAACA ATTAAAACAG AGTGCTCCAA TCAGCAGACC 
GTTGAACGGG ATTTGAAGGA TAACCGTGAG CTAAAACAGC TCGAAGATAA AGAAGCCAAG 
CTAAGAGAAA GTTGCCAGGT GCTGGACAAG CAGCTGGGTA ACCTTGACTT TCATAGCGTT 
AGCAAGGAGA AAGTGAATCT AACTAAGCAA AGGGATAAGG CCACAGTGCG TAAGGGCGAA 
CTGCTGGGCC AGCTGGGCGA GATTCACAGC CAGGTGAACA AGTTGCAGCG TGAAATCGAC 
GAGCCCAGAT TTAAGGAGTC TTTGAAAAAC TTCCGGAAGG CCAATTACGA GATAGAGGTG 
ACACGCCTCT GCATTGAGGA CTTGGGCCAA TATCGTTTAG CGTTGGAGTG GGCCCTTATC 
CAGTTTCACT CTGAAAAAAT GGAGATGATT AACCGTCTGA TCCGCGAGTA CTGGCGTAAG 
ATATATCGCG GAAATGACAT TGACTATATT CAGGTGAAGA CTGATGAGGT AAGCTCGGAT 
GCTTCGGCGG ATCGGCGAAA GACATACAAC TACAGAGTGG TCCAGTCGAA GAACTACTCA 
GAGATCGAGA TGCGGGGACG CTGTAGTGCT GGCCAGCGCG TCCTTGCCTC CCTTATTATC 
CGCTTGGCAC TGGCCGAGAC CTTCAGCAGT AATTGTGGTG TCCTAGCTCT GGATGAGCCC 
ACCACGAACT TGGATCGCGC CAATATAAAC TCGCTATGTG AAGCCCTGAA TTGCATCGTG 
GAGGAGCGCC AAAGCCAGTC CAACTTTATG CTCATCATCA TCACTCACGA CGAGAATTTC 
GTTTCGTCTC TGGGCAAGAT AACCTCCTAC CATCGGGTCT TCCGCAACGA GGAGTGCAAG 
TCGGTTATAC GGAGAGTGGA GGCGGGTCCA TCAAAGAAAG CTTTGATAGA TCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rad50 ( NM_001259532.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246461.1 Drosophila melanogaster rad50, transcript variant E (rad50), mRNA.

Price & Availability↑

CloneID	OFa12278
Clone ID Related Accession (Same CDS sequence)	NM_001259532.2
Accession Version	NM_001259532.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rad50, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001259532.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTCAA TTGAATCGCT GTCCATCCAG GGAATACGGA GTTTCGGCAC CTATGCCGAC 
GATCTTCAGA GTATAAAGTT TTCCTCGCCG GTTACCCTGA TTCTGGGCGA GAACGGATGC 
GGAAAGACGA CCGTGGTAGA GTGTCTCAAG TACGCCTTGA CCGGCGAGTG TCCGCCGGGC 
AGTGATCGGG GCAAGAGTTT CGTCCATGAC CCTAAGATCT TTGGGCTAAA CGAGGTGCTA 
GCGCAGATCA AGATGCAGGT GCGGGACAGG CGGGGTGCCC AAGTGTCCAT CTGCCGCACC 
ATGAAGGTGT CCAAGAAGCG CAACAAAATG TCCTTTGAAA CAATGGACTC CACCATCAAC 
TTCCTGACCG GCGCTGGACA GTCGAAGCGC GAAAAGCAGG ACTCGCTAAG CGGCCGCTCC 
GTGGATATCG ACGTGGCCAT CTCGGACTTC ATGGGTGTCT CCAAGGCTAT TATCAACAAT 
GTCCTGTTTT GCCATCAAGA GGACTCCAGT TGGCCGCTGG ACGAATCAAA AAAGTTAAAG 
GAAAAGTTCG ATGCCATATT CGGCATCACC GAGTACAATA AGGCGCTGGA TAAAATCATA 
AAGCTTCGCA AGGAGGCTAT GGAAGAGCTA AAAATAAAGG AGGCCAACAT AAAGCATGTG 
GCCTACCTCA AGCAGGAAAT GGAGGTTAAA ACTTTGAACC TACAAAAGGC GCAGAGGAAA 
TGCGACGCAA TTAAGGCGCA GTGCAGTGAG TGCGAGGAGG AAATGAAGCC GATTGAGGCC 
AGGCTGGTGG AAATCCGAAA TGTAGAGTTC GAAATTGGGA AGTATCAAGC GCAGAAAGTC 
GAAATGGACA CCAAGCACAA AAACTGCAAG GATCAGATAT CCACTCTAAC CCTGAAGATC 
AAGAAACCCT TTAGAGGTAC CCTGGATGAA TTGGATCAAG AGATAAGTAA CTTTGATCAG 
CGCATGCTAG AGATGCGTCA GAAACGCACC GAAGTGGAGG GGGATTTGTC GCAGATTAAG 
AGGTCCAGTG TCGCTGAGCA AGAAAAGTTG GGCACACAGG ACCGGAAGCA TTGCCTGGCT 
AAACAACGGC ATCAGAGCGA GCTGGCTTGT CGGGCACAGC TTCTGAAGCG GGTTAAGGAG 
TTCTGCCGGG AACTCCATAT ACCCATCGAT TGTGATCTTG TGGAGCAGCC TGAGAAAATG 
GGAGAAGTCC TTCGGGATAT CGAGGCGATG ATTATAACCA AGCACTGCGA AATCACAGAG 
ATAGTCGAGC AGAACGAGAA GGCCGATCGG AGCCGGCAAG TCAAGATCGA CGAGCTTCGC 
ATTGAGCTGA CCAAGTCGGA GCAAAGTGTA ACCGCGCAGG AGAAGCAGCG GGAGTCCAGC 
AAGCGAGAGA GTGAAACTCT TGGAGTGGAG ATAAAGAAAA TCGAAACATC CATGCAAGAC 
CTAAAGAAGC TTGAGAAAGA AATAAACGAG GTTAATGAGT TGTATGAGAG CGCTACGAAA 
AACATTGACC AACAGGCTAT AAAAGATGCC ATTGCCAGAA AGAAAGCCAG CATTGCCGAG 
AATCAGATTC AGTTTAAGAA GCTGGACGAA CAATTGACAT TCCTAGGCTC CATGGCCAAG 
CTAGTTGCCG AGTGTTCCCT GAAGCAAAAG GAGCTCGATA AAAAGAACCA AGAGGTACAT 
CGCGTGCGCA GCCGACACTC TGATCATTTC GGGAAGTTGT TCAAGGAACC AATTACCTGC 
AATTATCGCC GATCGATGCA GGTGGTCTAC GAAAAACTTC GTCGCGAGAT CCAAGAACTT 
AACGAGAAGG CAAACACTCA GAAACTAAAA GAACAGTCAT ACGAAATCAA GCGCAAAAAC 
CTCATTAGTG ATATTTCGCG AATGGAAAAG GAGTTAAAGG ACAGCGAGGA GTTAATATAT 
CAAAAGTGCC GGTCAACTCC GTATGATGAT CTGCTGGAGC GAAGTAAGAC GACTATTTCC 
AAATTGCAAT TCGACCATGG CGCCCTTAAA TCGTCCGAGG CCCTTTATAA GAAGTATATA 
CAGAAAATGG ATGAGGAGCC AAGTTGCCCA TTATGCCACC ACAACATGAC TAGTGATGAG 
GCGTGCGACT TGACTAGCGA GCTAACGGAT GAGATCCAAA AGTTGCCCGA TAATATCACT 
AGAGCTGAGA AAGCCTTAAA GGCGGAGCAG ATAAAATATG AGAACTTATT GCAACTTAAA 
CCCACCATTC TTAAGGTCAA GGAGCTTAAG GATTCGTTGC CACAAAAGAA GGAGGAACTG 
AAAAAGGTGG AGGAGCTGCT AGGAGACAGC GTCAGCGAAT ATGAAACATT AATTGCTCTT 
ATTGGGGAGC CAACTCATAA TATGGAGTTG GCGAATTCAA TGATGGGTGA TATGTCCCTA 
TTAGATGAGG CTTTAAAAGA TTCGGCGCGG CTCACAAAAG ATCTGGATCT GCAAAAGGGA 
CAGTTACCCG CTTCCTATGA TTCAAGTGTG TCTATGGATG ATCTGCAGGC GGAGAAGAGT 
AAGGTTTCAA AAGAGTTGGA GACGGAACGT AAGGAGTTGG AATCTGCTCA GAATGCAGTC 
CAGCAGCAAA TGGATGCCCT TAATCGTTTG CGCGAAAAGA AGAATAGTTT AAAAGACAGG 
CAAATACATC TTCGAGAGGG TCTTCAAAGC TTGCCACAGT TAAAGGAACG TTTGGAGAAG 
CTCAATAGTT TCCTTACAAC AGTAGCCTCT GAAATCAGCG AATTAAAGGC GAAAATCCAG 
CCTCTTAAGC TGAATTTACG AGCAGCCATA GAAGAAAAAG AGCGCTTGAA GAAAAGCGAA 
AGTGAAAAGT TAGCGCAGCT GAATTCCAAG TACAATTCCT ACAAAAGCAC AGATCACGAC 
ATACAAAGAT TGAACAAAGA AGCTGAGGAC TACGCCAAAT TGGATCTTCG AAATGAAATC 
AAAAAGCTTG ATGAAATCAT AATGGCCTCC AAAGATAAGT TAAGGAAATT GGCAACCGAA 
ATATCTTTAA AAACTGATGA GCTGGAAACA ATTAAAACAG AGTGCTCCAA TCAGCAGACC 
GTTGAACGGG ATTTGAAGGA TAACCGTGAG CTAAAACAGC TCGAAGATAA AGAAGCCAAG 
CTAAGAGAAA GTTGCCAGGT GCTGGACAAG CAGCTGGGTA ACCTTGACTT TCATAGCGTT 
AGCAAGGAGA AAGTGAATCT AACTAAGCAA AGGGATAAGG CCACAGTGCG TAAGGGCGAA 
CTGCTGGGCC AGCTGGGCGA GATTCACAGC CAGGTGAACA AGTTGCAGCG TGAAATCGAC 
GAGCCCAGAT TTAAGGAGTC TTTGAAAAAC TTCCGGAAGG CCAATTACGA GATAGAGGTG 
ACACGCCTCT GCATTGAGGA CTTGGGCCAA TATCGTTTAG CGTTGGAGTG GGCCCTTATC 
CAGTTTCACT CTGAAAAAAT GGAGATGATT AACCGTCTGA TCCGCGAGTA CTGGCGTAAG 
ATATATCGCG GAAATGACAT TGACTATATT CAGGTGAAGA CTGATGAGGT AAGCTCGGAT 
GCTTCGGCGG ATCGGCGAAA GACATACAAC TACAGAGTGG TCCAGTCGAA GAACTACTCA 
GAGATCGAGA TGCGGGGACG CTGTAGTGCT GGCCAGCGCG TCCTTGCCTC CCTTATTATC 
CGCTTGGCAC TGGCCGAGAC CTTCAGCAGT AATTGTGGTG TCCTAGCTCT GGATGAGCCC 
ACCACGAACT TGGATCGCGC CAATATAAAC TCGCTATGTG AAGCCCTGAA TTGCATCGTG 
GAGGAGCGCC AAAGCCAGTC CAACTTTATG CTCATCATCA TCACTCACGA CGAGAATTTC 
GTTTCGTCTC TGGGCAAGAT AACCTCCTAC CATCGGGTCT TCCGCAACGA GGAGTGCAAG 
TCGGTTATAC GGAGAGTGGA GGCGGGTCCA TCAAAGAAAG CTTTGATAGA TCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246461.1
CDS	87..4043
Translation	MSSIESLSIQGIRSFGTYADDLQSIKFSSPVTLILGENGCGKTTVVECLKYALTGECPPGSDRGKSFVHDPKIFGLNEVLAQIKMQVRDRRGAQVSICRTMKVSKKRNKMSFETMDSTINFLTGAGQSKREKQDSLSGRSVDIDVAISDFMGVSKAIINNVLFCHQEDSSWPLDESKKLKEKFDAIFGITEYNKALDKIIKLRKEAMEELKIKEANIKHVAYLKQEMEVKTLNLQKAQRKCDAIKAQCSECEEEMKPIEARLVEIRNVEFEIGKYQAQKVEMDTKHKNCKDQISTLTLKIKKPFRGTLDELDQEISNFDQRMLEMRQKRTEVEGDLSQIKRSSVAEQEKLGTQDRKHCLAKQRHQSELACRAQLLKRVKEFCRELHIPIDCDLVEQPEKMGEVLRDIEAMIITKHCEITEIVEQNEKADRSRQVKIDELRIELTKSEQSVTAQEKQRESSKRESETLGVEIKKIETSMQDLKKLEKEINEVNELYESATKNIDQQAIKDAIARKKASIAENQIQFKKLDEQLTFLGSMAKLVAECSLKQKELDKKNQEVHRVRSRHSDHFGKLFKEPITCNYRRSMQVVYEKLRREIQELNEKANTQKLKEQSYEIKRKNLISDISRMEKELKDSEELIYQKCRSTPYDDLLERSKTTISKLQFDHGALKSSEALYKKYIQKMDEEPSCPLCHHNMTSDEACDLTSELTDEIQKLPDNITRAEKALKAEQIKYENLLQLKPTILKVKELKDSLPQKKEELKKVEELLGDSVSEYETLIALIGEPTHNMELANSMMGDMSLLDEALKDSARLTKDLDLQKGQLPASYDSSVSMDDLQAEKSKVSKELETERKELESAQNAVQQQMDALNRLREKKNSLKDRQIHLREGLQSLPQLKERLEKLNSFLTTVASEISELKAKIQPLKLNLRAAIEEKERLKKSESEKLAQLNSKYNSYKSTDHDIQRLNKEAEDYAKLDLRNEIKKLDEIIMASKDKLRKLATEISLKTDELETIKTECSNQQTVERDLKDNRELKQLEDKEAKLRESCQVLDKQLGNLDFHSVSKEKVNLTKQRDKATVRKGELLGQLGEIHSQVNKLQREIDEPRFKESLKNFRKANYEIEVTRLCIEDLGQYRLALEWALIQFHSEKMEMINRLIREYWRKIYRGNDIDYIQVKTDEVSSDASADRRKTYNYRVVQSKNYSEIEMRGRCSAGQRVLASLIIRLALAETFSSNCGVLALDEPTTNLDRANINSLCEALNCIVEERQSQSNFMLIIITHDENFVSSLGKITSYHRVFRNEECKSVIRRVEAGPSKKALIDQ

Target ORF information:

RefSeq Version	NM_001259532.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rad50, transcript variant E (rad50), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259532.2

ORF Insert Sequence:

ATGTCGTCAA TTGAATCGCT GTCCATCCAG GGAATACGGA GTTTCGGCAC CTATGCCGAC 
GATCTTCAGA GTATAAAGTT TTCCTCGCCG GTTACCCTGA TTCTGGGCGA GAACGGATGC 
GGAAAGACGA CCGTGGTAGA GTGTCTCAAG TACGCCTTGA CCGGCGAGTG TCCGCCGGGC 
AGTGATCGGG GCAAGAGTTT CGTCCATGAC CCTAAGATCT TTGGGCTAAA CGAGGTGCTA 
GCGCAGATCA AGATGCAGGT GCGGGACAGG CGGGGTGCCC AAGTGTCCAT CTGCCGCACC 
ATGAAGGTGT CCAAGAAGCG CAACAAAATG TCCTTTGAAA CAATGGACTC CACCATCAAC 
TTCCTGACCG GCGCTGGACA GTCGAAGCGC GAAAAGCAGG ACTCGCTAAG CGGCCGCTCC 
GTGGATATCG ACGTGGCCAT CTCGGACTTC ATGGGTGTCT CCAAGGCTAT TATCAACAAT 
GTCCTGTTTT GCCATCAAGA GGACTCCAGT TGGCCGCTGG ACGAATCAAA AAAGTTAAAG 
GAAAAGTTCG ATGCCATATT CGGCATCACC GAGTACAATA AGGCGCTGGA TAAAATCATA 
AAGCTTCGCA AGGAGGCTAT GGAAGAGCTA AAAATAAAGG AGGCCAACAT AAAGCATGTG 
GCCTACCTCA AGCAGGAAAT GGAGGTTAAA ACTTTGAACC TACAAAAGGC GCAGAGGAAA 
TGCGACGCAA TTAAGGCGCA GTGCAGTGAG TGCGAGGAGG AAATGAAGCC GATTGAGGCC 
AGGCTGGTGG AAATCCGAAA TGTAGAGTTC GAAATTGGGA AGTATCAAGC GCAGAAAGTC 
GAAATGGACA CCAAGCACAA AAACTGCAAG GATCAGATAT CCACTCTAAC CCTGAAGATC 
AAGAAACCCT TTAGAGGTAC CCTGGATGAA TTGGATCAAG AGATAAGTAA CTTTGATCAG 
CGCATGCTAG AGATGCGTCA GAAACGCACC GAAGTGGAGG GGGATTTGTC GCAGATTAAG 
AGGTCCAGTG TCGCTGAGCA AGAAAAGTTG GGCACACAGG ACCGGAAGCA TTGCCTGGCT 
AAACAACGGC ATCAGAGCGA GCTGGCTTGT CGGGCACAGC TTCTGAAGCG GGTTAAGGAG 
TTCTGCCGGG AACTCCATAT ACCCATCGAT TGTGATCTTG TGGAGCAGCC TGAGAAAATG 
GGAGAAGTCC TTCGGGATAT CGAGGCGATG ATTATAACCA AGCACTGCGA AATCACAGAG 
ATAGTCGAGC AGAACGAGAA GGCCGATCGG AGCCGGCAAG TCAAGATCGA CGAGCTTCGC 
ATTGAGCTGA CCAAGTCGGA GCAAAGTGTA ACCGCGCAGG AGAAGCAGCG GGAGTCCAGC 
AAGCGAGAGA GTGAAACTCT TGGAGTGGAG ATAAAGAAAA TCGAAACATC CATGCAAGAC 
CTAAAGAAGC TTGAGAAAGA AATAAACGAG GTTAATGAGT TGTATGAGAG CGCTACGAAA 
AACATTGACC AACAGGCTAT AAAAGATGCC ATTGCCAGAA AGAAAGCCAG CATTGCCGAG 
AATCAGATTC AGTTTAAGAA GCTGGACGAA CAATTGACAT TCCTAGGCTC CATGGCCAAG 
CTAGTTGCCG AGTGTTCCCT GAAGCAAAAG GAGCTCGATA AAAAGAACCA AGAGGTACAT 
CGCGTGCGCA GCCGACACTC TGATCATTTC GGGAAGTTGT TCAAGGAACC AATTACCTGC 
AATTATCGCC GATCGATGCA GGTGGTCTAC GAAAAACTTC GTCGCGAGAT CCAAGAACTT 
AACGAGAAGG CAAACACTCA GAAACTAAAA GAACAGTCAT ACGAAATCAA GCGCAAAAAC 
CTCATTAGTG ATATTTCGCG AATGGAAAAG GAGTTAAAGG ACAGCGAGGA GTTAATATAT 
CAAAAGTGCC GGTCAACTCC GTATGATGAT CTGCTGGAGC GAAGTAAGAC GACTATTTCC 
AAATTGCAAT TCGACCATGG CGCCCTTAAA TCGTCCGAGG CCCTTTATAA GAAGTATATA 
CAGAAAATGG ATGAGGAGCC AAGTTGCCCA TTATGCCACC ACAACATGAC TAGTGATGAG 
GCGTGCGACT TGACTAGCGA GCTAACGGAT GAGATCCAAA AGTTGCCCGA TAATATCACT 
AGAGCTGAGA AAGCCTTAAA GGCGGAGCAG ATAAAATATG AGAACTTATT GCAACTTAAA 
CCCACCATTC TTAAGGTCAA GGAGCTTAAG GATTCGTTGC CACAAAAGAA GGAGGAACTG 
AAAAAGGTGG AGGAGCTGCT AGGAGACAGC GTCAGCGAAT ATGAAACATT AATTGCTCTT 
ATTGGGGAGC CAACTCATAA TATGGAGTTG GCGAATTCAA TGATGGGTGA TATGTCCCTA 
TTAGATGAGG CTTTAAAAGA TTCGGCGCGG CTCACAAAAG ATCTGGATCT GCAAAAGGGA 
CAGTTACCCG CTTCCTATGA TTCAAGTGTG TCTATGGATG ATCTGCAGGC GGAGAAGAGT 
AAGGTTTCAA AAGAGTTGGA GACGGAACGT AAGGAGTTGG AATCTGCTCA GAATGCAGTC 
CAGCAGCAAA TGGATGCCCT TAATCGTTTG CGCGAAAAGA AGAATAGTTT AAAAGACAGG 
CAAATACATC TTCGAGAGGG TCTTCAAAGC TTGCCACAGT TAAAGGAACG TTTGGAGAAG 
CTCAATAGTT TCCTTACAAC AGTAGCCTCT GAAATCAGCG AATTAAAGGC GAAAATCCAG 
CCTCTTAAGC TGAATTTACG AGCAGCCATA GAAGAAAAAG AGCGCTTGAA GAAAAGCGAA 
AGTGAAAAGT TAGCGCAGCT GAATTCCAAG TACAATTCCT ACAAAAGCAC AGATCACGAC 
ATACAAAGAT TGAACAAAGA AGCTGAGGAC TACGCCAAAT TGGATCTTCG AAATGAAATC 
AAAAAGCTTG ATGAAATCAT AATGGCCTCC AAAGATAAGT TAAGGAAATT GGCAACCGAA 
ATATCTTTAA AAACTGATGA GCTGGAAACA ATTAAAACAG AGTGCTCCAA TCAGCAGACC 
GTTGAACGGG ATTTGAAGGA TAACCGTGAG CTAAAACAGC TCGAAGATAA AGAAGCCAAG 
CTAAGAGAAA GTTGCCAGGT GCTGGACAAG CAGCTGGGTA ACCTTGACTT TCATAGCGTT 
AGCAAGGAGA AAGTGAATCT AACTAAGCAA AGGGATAAGG CCACAGTGCG TAAGGGCGAA 
CTGCTGGGCC AGCTGGGCGA GATTCACAGC CAGGTGAACA AGTTGCAGCG TGAAATCGAC 
GAGCCCAGAT TTAAGGAGTC TTTGAAAAAC TTCCGGAAGG CCAATTACGA GATAGAGGTG 
ACACGCCTCT GCATTGAGGA CTTGGGCCAA TATCGTTTAG CGTTGGAGTG GGCCCTTATC 
CAGTTTCACT CTGAAAAAAT GGAGATGATT AACCGTCTGA TCCGCGAGTA CTGGCGTAAG 
ATATATCGCG GAAATGACAT TGACTATATT CAGGTGAAGA CTGATGAGGT AAGCTCGGAT 
GCTTCGGCGG ATCGGCGAAA GACATACAAC TACAGAGTGG TCCAGTCGAA GAACTACTCA 
GAGATCGAGA TGCGGGGACG CTGTAGTGCT GGCCAGCGCG TCCTTGCCTC CCTTATTATC 
CGCTTGGCAC TGGCCGAGAC CTTCAGCAGT AATTGTGGTG TCCTAGCTCT GGATGAGCCC 
ACCACGAACT TGGATCGCGC CAATATAAAC TCGCTATGTG AAGCCCTGAA TTGCATCGTG 
GAGGAGCGCC AAAGCCAGTC CAACTTTATG CTCATCATCA TCACTCACGA CGAGAATTTC 
GTTTCGTCTC TGGGCAAGAT AACCTCCTAC CATCGGGTCT TCCGCAACGA GGAGTGCAAG 
TCGGTTATAC GGAGAGTGGA GGCGGGTCCA TCAAAGAAAG CTTTGATAGA TCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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rad50 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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