Rtf1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rtf1
Entrez Gene ID	37554
Full Name	CG10955 gene product from transcript CG10955-RB
Synonyms	CG10955,Dmel\CG10955,dRtf1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 58D4-58D4

mRNA and Protein(s)

mRNA	Protein	Name
NM_137821.3	NP_611665.1	Rtf1, isoform A
NM_001299788.1	NP_001286717.1	Rtf1, isoform B

Pathways from BioSystems

Homologies

Bos taurus (cattle)	RTF1	NP_001179414.1
Mus musculus (house mouse)	Rtf1	NP_084388.2
Drosophila melanogaster (fruit fly)	Rtf1	NP_611665.1
	RTF1	XP_001100507.1
Gallus gallus (chicken)	RTF1	XP_421139.3
Pan troglodytes (chimpanzee)	RTF1	XP_001142072.1
Canis lupus familiaris (dog)	RTF1	XP_544630.2
Danio rerio (zebrafish)	rtf1	NP_001077046.1
Xenopus tropicalis (tropical clawed frog)	rtf1	NP_001005068.1
Homo sapiens (human)	RTF1	NP_055953.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the dRtf1 component of the Paf1 complex functions in Notch signaling
Title: Drosophila Rtf1 functions in histone methylation, gene expression, and Notch signaling.

The following Rtf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rtf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10919	NM_137821.3 Latest version!	Drosophila melanogaster Rtf1, transcript variant A (Rtf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa10919	NM_001299788.1 Latest version!	Drosophila melanogaster Rtf1, transcript variant B (Rtf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rtf1 ( NM_137821.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611665.1 Drosophila melanogaster Rtf1, transcript variant A (Rtf1), mRNA.

Price & Availability↑

CloneID	OFa10919
Clone ID Related Accession (Same CDS sequence)	NM_137821.3 , NM_001299788.1
Accession Version	NM_137821.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rtf1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137821.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCAAAC GGCGGACGCA ATCCCTGATC GACTCCAACT CCAGCGATAG CGACAGCGAG 
TCCGAGACCA ATCTGGAATC GGACCTGATG TCGCTCGCCA AGAAGCGAAA GAAGCCCCAA 
ACGGCGGCCA AGTCAAGTTC CCGCTCCGAC TCTGATTCCG ACTGGGCCAA CAACAAAGCT 
GGAGCTCCCT CGTCCAAGAA GAAGAAGCGT CAGAAGCCCA GCAGAGACTC CAGCTCCTCG 
GAGTCCAATT GGGATGACGA CAGTCAGGAT GAGAGGCAGC CAGCGAGGCA GAGTCCTGCA 
CAGACGCAAC AGGAGCACAA GCCCCCGGAG CAGGCCTCCC AGCCCGCCCA GCTCAGCGAG 
CAGGAGGAGG GCGAGGTCTC CGATAGCGAT TCCGACAAGT CCAAGTCCAA CTCCTCCTCA 
TCCGGCTCCG ACTCCTCTAG TTCTTCCTCC AGCTCCGACT CGGAATTTGA CGACGGGTTC 
GATGATGACC TCATGGGCGA TGATGAGGAT CGAAGGCGTC TCAATGGTTT GTCCGAGAAG 
GAGCGTGAAA CTGAAATTTA CAAGCGCATT GAGCAGCGCG AGATTATGCG AACGCGCTGG 
GAAATCGAAC GGAAACTGAA GCTGGCAAGG CGCGGCGAGA AAAACCAAGA AAAGTCCAAG 
AACAAAGGAG AACGGGCCAA GAAGAAGAAG GAAAAGCGCG AGAAGAAGGC GAGGAAAGCC 
AGGGAAGCCC AGGCGCCCTT GCCAACTCAA GCTTCCACAT CCACACTTTT AGATGTCGAA 
CCCAAGCCCA GCAACGAAGT ACGGTCAGCG AGTCCGTTGT CCACGCCTGC CCTTAACCGA 
GATGCGGCTT CCACTTCAGC GGCAGTGGCC AGTATTATGC CCGACGATGC AGCTTCCTCC 
GCTGGCGTCT CTGATTACTT TGATCACAAG GAACGTTCCA AGGAGCGCAA GAAGAACGTG 
GAGGCCAACA AGACGGACGA CAAGAGGAGC AATGCCATGG CCTTATTAAA AGCCAAAAGA 
GAGGGCAAGG CTAAAAGAGA AGAGGAGGAA GCCAAACGCA TGGCAGAAAA AGATCGTGAT 
GACGACAAAG AGGAACTCGA TAGCGTATCT GGTTGTAAAT CGGCCGTGAA ACTGAAAGCC 
TCTGAAATAT ACTCTGACGA CTCAGGCAGC AGCGATTGGG ATGAGGAAGA AAAGCCCGCG 
GGCAAACGTT CACGTTCCAA CAGCAGCAAG GCCTCCAGCG AATCCGAGGA CGAAGAAAAG 
GCGCCTCAGC GGCCCGTTTT TATAACCACA CGCGAGGATC TTAACAAATT GCGTCTATCT 
CGCTACAAAA TGGAGCGATT TGTGAACTTG CCAATCTTTG AGAGCACTGT ACTCAACTGC 
TTTGTCCGGA TAAGTATCGG AAACAATGGC CAGAAACCCG TGTATCGAGT GGCCGAAATT 
GTGGGAGTGG TTGAGACGGG GAAGATCTAC AGTCTGGGCA CTACACGAAC CAATCGTGGA 
TTGAGACTTA AGCACGGAAC CCAGGAGCGG GTCTTCCGAC TCGAGTTCAT TTCGAACCAG 
GAGTTTACGG AGAACGAGTT CAACAAGTGG AACGAGGTCT GCCAGCAGTC GCACGTCCAG 
ATGCCAACGA TTGATCTAAT AGCGATCAAG CAGAACGACA TCAAAAAAGC ATTGAACTAC 
GAATTCAAGG ACGAGGACGT GGACAAGATC GTGGAGGAGA AGAACCGCTT CCGGAACCGA 
CCCACCAATT ATGCGATGAA GAAAACCTGC CTGATGAAGG AGCGAGATGC AGCCATGTTG 
CGGGGTGACT ACGACATTGC ACAGGATCTG GGACAGCAAA TCGACGAGCT GGAGAACCGA 
GCCTCTGAGC TCGACAAAAG AAGATCCCAT ACCCTCAATC TGATCTCCTA CATAAACGAC 
AGGAACAGGA AGAAGAATGT GGAGGACGCT GAGAAGGCAA TTTTAGAGGA GGCTCGGGCC 
AACAAAGGCC TCAAGATCTC GGATCCCTTC ACACGTCGCA TTACTCAGCC GCGCATGGGC 
TTCAAGGGTG CCAAGAAGGA CGAAGATGAC ATGCAACTGG CACCACTGCC GCCGCCACCC 
CCAGGAAAAA AGAGGCCCAA CGAAGCAGGA ACTTCGAGTG CAAGCGTCAG GTCCACGGAC 
TCCAAGGACT ACAGTCTCTA CTCACTGCAT GACTTTGACA TTGACCTAGA TGTTCCGCTA 
CCAGTCAATA CGAATTCAGT GCCCAAGCCG GCTAGCAAAC CAGCTGAAAC AGTCTCCAAA 
CGTTCGCTGA ATCTAGAGGA TTACAAAAAA AAGCGCGGTC TTATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611665.1
CDS	103..2430
Translation	MGKRRTQSLIDSNSSDSDSESETNLESDLMSLAKKRKKPQTAAKSSSRSDSDSDWANNKAGAPSSKKKKRQKPSRDSSSSESNWDDDSQDERQPARQSPAQTQQEHKPPEQASQPAQLSEQEEGEVSDSDSDKSKSNSSSSGSDSSSSSSSSDSEFDDGFDDDLMGDDEDRRRLNGLSEKERETEIYKRIEQREIMRTRWEIERKLKLARRGEKNQEKSKNKGERAKKKKEKREKKARKAREAQAPLPTQASTSTLLDVEPKPSNEVRSASPLSTPALNRDAASTSAAVASIMPDDAASSAGVSDYFDHKERSKERKKNVEANKTDDKRSNAMALLKAKREGKAKREEEEAKRMAEKDRDDDKEELDSVSGCKSAVKLKASEIYSDDSGSSDWDEEEKPAGKRSRSNSSKASSESEDEEKAPQRPVFITTREDLNKLRLSRYKMERFVNLPIFESTVLNCFVRISIGNNGQKPVYRVAEIVGVVETGKIYSLGTTRTNRGLRLKHGTQERVFRLEFISNQEFTENEFNKWNEVCQQSHVQMPTIDLIAIKQNDIKKALNYEFKDEDVDKIVEEKNRFRNRPTNYAMKKTCLMKERDAAMLRGDYDIAQDLGQQIDELENRASELDKRRSHTLNLISYINDRNRKKNVEDAEKAILEEARANKGLKISDPFTRRITQPRMGFKGAKKDEDDMQLAPLPPPPPGKKRPNEAGTSSASVRSTDSKDYSLYSLHDFDIDLDVPLPVNTNSVPKPASKPAETVSKRSLNLEDYKKKRGLI

Target ORF information:

RefSeq Version	NM_137821.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rtf1, transcript variant A (Rtf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137821.3

ORF Insert Sequence:

ATGGGCAAAC GGCGGACGCA ATCCCTGATC GACTCCAACT CCAGCGATAG CGACAGCGAG 
TCCGAGACCA ATCTGGAATC GGACCTGATG TCGCTCGCCA AGAAGCGAAA GAAGCCCCAA 
ACGGCGGCCA AGTCAAGTTC CCGCTCCGAC TCTGATTCCG ACTGGGCCAA CAACAAAGCT 
GGAGCTCCCT CGTCCAAGAA GAAGAAGCGT CAGAAGCCCA GCAGAGACTC CAGCTCCTCG 
GAGTCCAATT GGGATGACGA CAGTCAGGAT GAGAGGCAGC CAGCGAGGCA GAGTCCTGCA 
CAGACGCAAC AGGAGCACAA GCCCCCGGAG CAGGCCTCCC AGCCCGCCCA GCTCAGCGAG 
CAGGAGGAGG GCGAGGTCTC CGATAGCGAT TCCGACAAGT CCAAGTCCAA CTCCTCCTCA 
TCCGGCTCCG ACTCCTCTAG TTCTTCCTCC AGCTCCGACT CGGAATTTGA CGACGGGTTC 
GATGATGACC TCATGGGCGA TGATGAGGAT CGAAGGCGTC TCAATGGTTT GTCCGAGAAG 
GAGCGTGAAA CTGAAATTTA CAAGCGCATT GAGCAGCGCG AGATTATGCG AACGCGCTGG 
GAAATCGAAC GGAAACTGAA GCTGGCAAGG CGCGGCGAGA AAAACCAAGA AAAGTCCAAG 
AACAAAGGAG AACGGGCCAA GAAGAAGAAG GAAAAGCGCG AGAAGAAGGC GAGGAAAGCC 
AGGGAAGCCC AGGCGCCCTT GCCAACTCAA GCTTCCACAT CCACACTTTT AGATGTCGAA 
CCCAAGCCCA GCAACGAAGT ACGGTCAGCG AGTCCGTTGT CCACGCCTGC CCTTAACCGA 
GATGCGGCTT CCACTTCAGC GGCAGTGGCC AGTATTATGC CCGACGATGC AGCTTCCTCC 
GCTGGCGTCT CTGATTACTT TGATCACAAG GAACGTTCCA AGGAGCGCAA GAAGAACGTG 
GAGGCCAACA AGACGGACGA CAAGAGGAGC AATGCCATGG CCTTATTAAA AGCCAAAAGA 
GAGGGCAAGG CTAAAAGAGA AGAGGAGGAA GCCAAACGCA TGGCAGAAAA AGATCGTGAT 
GACGACAAAG AGGAACTCGA TAGCGTATCT GGTTGTAAAT CGGCCGTGAA ACTGAAAGCC 
TCTGAAATAT ACTCTGACGA CTCAGGCAGC AGCGATTGGG ATGAGGAAGA AAAGCCCGCG 
GGCAAACGTT CACGTTCCAA CAGCAGCAAG GCCTCCAGCG AATCCGAGGA CGAAGAAAAG 
GCGCCTCAGC GGCCCGTTTT TATAACCACA CGCGAGGATC TTAACAAATT GCGTCTATCT 
CGCTACAAAA TGGAGCGATT TGTGAACTTG CCAATCTTTG AGAGCACTGT ACTCAACTGC 
TTTGTCCGGA TAAGTATCGG AAACAATGGC CAGAAACCCG TGTATCGAGT GGCCGAAATT 
GTGGGAGTGG TTGAGACGGG GAAGATCTAC AGTCTGGGCA CTACACGAAC CAATCGTGGA 
TTGAGACTTA AGCACGGAAC CCAGGAGCGG GTCTTCCGAC TCGAGTTCAT TTCGAACCAG 
GAGTTTACGG AGAACGAGTT CAACAAGTGG AACGAGGTCT GCCAGCAGTC GCACGTCCAG 
ATGCCAACGA TTGATCTAAT AGCGATCAAG CAGAACGACA TCAAAAAAGC ATTGAACTAC 
GAATTCAAGG ACGAGGACGT GGACAAGATC GTGGAGGAGA AGAACCGCTT CCGGAACCGA 
CCCACCAATT ATGCGATGAA GAAAACCTGC CTGATGAAGG AGCGAGATGC AGCCATGTTG 
CGGGGTGACT ACGACATTGC ACAGGATCTG GGACAGCAAA TCGACGAGCT GGAGAACCGA 
GCCTCTGAGC TCGACAAAAG AAGATCCCAT ACCCTCAATC TGATCTCCTA CATAAACGAC 
AGGAACAGGA AGAAGAATGT GGAGGACGCT GAGAAGGCAA TTTTAGAGGA GGCTCGGGCC 
AACAAAGGCC TCAAGATCTC GGATCCCTTC ACACGTCGCA TTACTCAGCC GCGCATGGGC 
TTCAAGGGTG CCAAGAAGGA CGAAGATGAC ATGCAACTGG CACCACTGCC GCCGCCACCC 
CCAGGAAAAA AGAGGCCCAA CGAAGCAGGA ACTTCGAGTG CAAGCGTCAG GTCCACGGAC 
TCCAAGGACT ACAGTCTCTA CTCACTGCAT GACTTTGACA TTGACCTAGA TGTTCCGCTA 
CCAGTCAATA CGAATTCAGT GCCCAAGCCG GCTAGCAAAC CAGCTGAAAC AGTCTCCAAA 
CGTTCGCTGA ATCTAGAGGA TTACAAAAAA AAGCGCGGTC TTATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rtf1 ( NM_001299788.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286717.1 Drosophila melanogaster Rtf1, transcript variant B (Rtf1), mRNA.

Price & Availability↑

CloneID	OFa10919
Clone ID Related Accession (Same CDS sequence)	NM_137821.3 , NM_001299788.1
Accession Version	NM_001299788.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rtf1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCAAAC GGCGGACGCA ATCCCTGATC GACTCCAACT CCAGCGATAG CGACAGCGAG 
TCCGAGACCA ATCTGGAATC GGACCTGATG TCGCTCGCCA AGAAGCGAAA GAAGCCCCAA 
ACGGCGGCCA AGTCAAGTTC CCGCTCCGAC TCTGATTCCG ACTGGGCCAA CAACAAAGCT 
GGAGCTCCCT CGTCCAAGAA GAAGAAGCGT CAGAAGCCCA GCAGAGACTC CAGCTCCTCG 
GAGTCCAATT GGGATGACGA CAGTCAGGAT GAGAGGCAGC CAGCGAGGCA GAGTCCTGCA 
CAGACGCAAC AGGAGCACAA GCCCCCGGAG CAGGCCTCCC AGCCCGCCCA GCTCAGCGAG 
CAGGAGGAGG GCGAGGTCTC CGATAGCGAT TCCGACAAGT CCAAGTCCAA CTCCTCCTCA 
TCCGGCTCCG ACTCCTCTAG TTCTTCCTCC AGCTCCGACT CGGAATTTGA CGACGGGTTC 
GATGATGACC TCATGGGCGA TGATGAGGAT CGAAGGCGTC TCAATGGTTT GTCCGAGAAG 
GAGCGTGAAA CTGAAATTTA CAAGCGCATT GAGCAGCGCG AGATTATGCG AACGCGCTGG 
GAAATCGAAC GGAAACTGAA GCTGGCAAGG CGCGGCGAGA AAAACCAAGA AAAGTCCAAG 
AACAAAGGAG AACGGGCCAA GAAGAAGAAG GAAAAGCGCG AGAAGAAGGC GAGGAAAGCC 
AGGGAAGCCC AGGCGCCCTT GCCAACTCAA GCTTCCACAT CCACACTTTT AGATGTCGAA 
CCCAAGCCCA GCAACGAAGT ACGGTCAGCG AGTCCGTTGT CCACGCCTGC CCTTAACCGA 
GATGCGGCTT CCACTTCAGC GGCAGTGGCC AGTATTATGC CCGACGATGC AGCTTCCTCC 
GCTGGCGTCT CTGATTACTT TGATCACAAG GAACGTTCCA AGGAGCGCAA GAAGAACGTG 
GAGGCCAACA AGACGGACGA CAAGAGGAGC AATGCCATGG CCTTATTAAA AGCCAAAAGA 
GAGGGCAAGG CTAAAAGAGA AGAGGAGGAA GCCAAACGCA TGGCAGAAAA AGATCGTGAT 
GACGACAAAG AGGAACTCGA TAGCGTATCT GGTTGTAAAT CGGCCGTGAA ACTGAAAGCC 
TCTGAAATAT ACTCTGACGA CTCAGGCAGC AGCGATTGGG ATGAGGAAGA AAAGCCCGCG 
GGCAAACGTT CACGTTCCAA CAGCAGCAAG GCCTCCAGCG AATCCGAGGA CGAAGAAAAG 
GCGCCTCAGC GGCCCGTTTT TATAACCACA CGCGAGGATC TTAACAAATT GCGTCTATCT 
CGCTACAAAA TGGAGCGATT TGTGAACTTG CCAATCTTTG AGAGCACTGT ACTCAACTGC 
TTTGTCCGGA TAAGTATCGG AAACAATGGC CAGAAACCCG TGTATCGAGT GGCCGAAATT 
GTGGGAGTGG TTGAGACGGG GAAGATCTAC AGTCTGGGCA CTACACGAAC CAATCGTGGA 
TTGAGACTTA AGCACGGAAC CCAGGAGCGG GTCTTCCGAC TCGAGTTCAT TTCGAACCAG 
GAGTTTACGG AGAACGAGTT CAACAAGTGG AACGAGGTCT GCCAGCAGTC GCACGTCCAG 
ATGCCAACGA TTGATCTAAT AGCGATCAAG CAGAACGACA TCAAAAAAGC ATTGAACTAC 
GAATTCAAGG ACGAGGACGT GGACAAGATC GTGGAGGAGA AGAACCGCTT CCGGAACCGA 
CCCACCAATT ATGCGATGAA GAAAACCTGC CTGATGAAGG AGCGAGATGC AGCCATGTTG 
CGGGGTGACT ACGACATTGC ACAGGATCTG GGACAGCAAA TCGACGAGCT GGAGAACCGA 
GCCTCTGAGC TCGACAAAAG AAGATCCCAT ACCCTCAATC TGATCTCCTA CATAAACGAC 
AGGAACAGGA AGAAGAATGT GGAGGACGCT GAGAAGGCAA TTTTAGAGGA GGCTCGGGCC 
AACAAAGGCC TCAAGATCTC GGATCCCTTC ACACGTCGCA TTACTCAGCC GCGCATGGGC 
TTCAAGGGTG CCAAGAAGGA CGAAGATGAC ATGCAACTGG CACCACTGCC GCCGCCACCC 
CCAGGAAAAA AGAGGCCCAA CGAAGCAGGA ACTTCGAGTG CAAGCGTCAG GTCCACGGAC 
TCCAAGGACT ACAGTCTCTA CTCACTGCAT GACTTTGACA TTGACCTAGA TGTTCCGCTA 
CCAGTCAATA CGAATTCAGT GCCCAAGCCG GCTAGCAAAC CAGCTGAAAC AGTCTCCAAA 
CGTTCGCTGA ATCTAGAGGA TTACAAAAAA AAGCGCGGTC TTATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286717.1
CDS	103..2430
Translation	MGKRRTQSLIDSNSSDSDSESETNLESDLMSLAKKRKKPQTAAKSSSRSDSDSDWANNKAGAPSSKKKKRQKPSRDSSSSESNWDDDSQDERQPARQSPAQTQQEHKPPEQASQPAQLSEQEEGEVSDSDSDKSKSNSSSSGSDSSSSSSSSDSEFDDGFDDDLMGDDEDRRRLNGLSEKERETEIYKRIEQREIMRTRWEIERKLKLARRGEKNQEKSKNKGERAKKKKEKREKKARKAREAQAPLPTQASTSTLLDVEPKPSNEVRSASPLSTPALNRDAASTSAAVASIMPDDAASSAGVSDYFDHKERSKERKKNVEANKTDDKRSNAMALLKAKREGKAKREEEEAKRMAEKDRDDDKEELDSVSGCKSAVKLKASEIYSDDSGSSDWDEEEKPAGKRSRSNSSKASSESEDEEKAPQRPVFITTREDLNKLRLSRYKMERFVNLPIFESTVLNCFVRISIGNNGQKPVYRVAEIVGVVETGKIYSLGTTRTNRGLRLKHGTQERVFRLEFISNQEFTENEFNKWNEVCQQSHVQMPTIDLIAIKQNDIKKALNYEFKDEDVDKIVEEKNRFRNRPTNYAMKKTCLMKERDAAMLRGDYDIAQDLGQQIDELENRASELDKRRSHTLNLISYINDRNRKKNVEDAEKAILEEARANKGLKISDPFTRRITQPRMGFKGAKKDEDDMQLAPLPPPPPGKKRPNEAGTSSASVRSTDSKDYSLYSLHDFDIDLDVPLPVNTNSVPKPASKPAETVSKRSLNLEDYKKKRGLI

Target ORF information:

RefSeq Version	NM_001299788.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rtf1, transcript variant B (Rtf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299788.1

ORF Insert Sequence:

ATGGGCAAAC GGCGGACGCA ATCCCTGATC GACTCCAACT CCAGCGATAG CGACAGCGAG 
TCCGAGACCA ATCTGGAATC GGACCTGATG TCGCTCGCCA AGAAGCGAAA GAAGCCCCAA 
ACGGCGGCCA AGTCAAGTTC CCGCTCCGAC TCTGATTCCG ACTGGGCCAA CAACAAAGCT 
GGAGCTCCCT CGTCCAAGAA GAAGAAGCGT CAGAAGCCCA GCAGAGACTC CAGCTCCTCG 
GAGTCCAATT GGGATGACGA CAGTCAGGAT GAGAGGCAGC CAGCGAGGCA GAGTCCTGCA 
CAGACGCAAC AGGAGCACAA GCCCCCGGAG CAGGCCTCCC AGCCCGCCCA GCTCAGCGAG 
CAGGAGGAGG GCGAGGTCTC CGATAGCGAT TCCGACAAGT CCAAGTCCAA CTCCTCCTCA 
TCCGGCTCCG ACTCCTCTAG TTCTTCCTCC AGCTCCGACT CGGAATTTGA CGACGGGTTC 
GATGATGACC TCATGGGCGA TGATGAGGAT CGAAGGCGTC TCAATGGTTT GTCCGAGAAG 
GAGCGTGAAA CTGAAATTTA CAAGCGCATT GAGCAGCGCG AGATTATGCG AACGCGCTGG 
GAAATCGAAC GGAAACTGAA GCTGGCAAGG CGCGGCGAGA AAAACCAAGA AAAGTCCAAG 
AACAAAGGAG AACGGGCCAA GAAGAAGAAG GAAAAGCGCG AGAAGAAGGC GAGGAAAGCC 
AGGGAAGCCC AGGCGCCCTT GCCAACTCAA GCTTCCACAT CCACACTTTT AGATGTCGAA 
CCCAAGCCCA GCAACGAAGT ACGGTCAGCG AGTCCGTTGT CCACGCCTGC CCTTAACCGA 
GATGCGGCTT CCACTTCAGC GGCAGTGGCC AGTATTATGC CCGACGATGC AGCTTCCTCC 
GCTGGCGTCT CTGATTACTT TGATCACAAG GAACGTTCCA AGGAGCGCAA GAAGAACGTG 
GAGGCCAACA AGACGGACGA CAAGAGGAGC AATGCCATGG CCTTATTAAA AGCCAAAAGA 
GAGGGCAAGG CTAAAAGAGA AGAGGAGGAA GCCAAACGCA TGGCAGAAAA AGATCGTGAT 
GACGACAAAG AGGAACTCGA TAGCGTATCT GGTTGTAAAT CGGCCGTGAA ACTGAAAGCC 
TCTGAAATAT ACTCTGACGA CTCAGGCAGC AGCGATTGGG ATGAGGAAGA AAAGCCCGCG 
GGCAAACGTT CACGTTCCAA CAGCAGCAAG GCCTCCAGCG AATCCGAGGA CGAAGAAAAG 
GCGCCTCAGC GGCCCGTTTT TATAACCACA CGCGAGGATC TTAACAAATT GCGTCTATCT 
CGCTACAAAA TGGAGCGATT TGTGAACTTG CCAATCTTTG AGAGCACTGT ACTCAACTGC 
TTTGTCCGGA TAAGTATCGG AAACAATGGC CAGAAACCCG TGTATCGAGT GGCCGAAATT 
GTGGGAGTGG TTGAGACGGG GAAGATCTAC AGTCTGGGCA CTACACGAAC CAATCGTGGA 
TTGAGACTTA AGCACGGAAC CCAGGAGCGG GTCTTCCGAC TCGAGTTCAT TTCGAACCAG 
GAGTTTACGG AGAACGAGTT CAACAAGTGG AACGAGGTCT GCCAGCAGTC GCACGTCCAG 
ATGCCAACGA TTGATCTAAT AGCGATCAAG CAGAACGACA TCAAAAAAGC ATTGAACTAC 
GAATTCAAGG ACGAGGACGT GGACAAGATC GTGGAGGAGA AGAACCGCTT CCGGAACCGA 
CCCACCAATT ATGCGATGAA GAAAACCTGC CTGATGAAGG AGCGAGATGC AGCCATGTTG 
CGGGGTGACT ACGACATTGC ACAGGATCTG GGACAGCAAA TCGACGAGCT GGAGAACCGA 
GCCTCTGAGC TCGACAAAAG AAGATCCCAT ACCCTCAATC TGATCTCCTA CATAAACGAC 
AGGAACAGGA AGAAGAATGT GGAGGACGCT GAGAAGGCAA TTTTAGAGGA GGCTCGGGCC 
AACAAAGGCC TCAAGATCTC GGATCCCTTC ACACGTCGCA TTACTCAGCC GCGCATGGGC 
TTCAAGGGTG CCAAGAAGGA CGAAGATGAC ATGCAACTGG CACCACTGCC GCCGCCACCC 
CCAGGAAAAA AGAGGCCCAA CGAAGCAGGA ACTTCGAGTG CAAGCGTCAG GTCCACGGAC 
TCCAAGGACT ACAGTCTCTA CTCACTGCAT GACTTTGACA TTGACCTAGA TGTTCCGCTA 
CCAGTCAATA CGAATTCAGT GCCCAAGCCG GCTAGCAAAC CAGCTGAAAC AGTCTCCAAA 
CGTTCGCTGA ATCTAGAGGA TTACAAAAAA AAGCGCGGTC TTATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Rtf1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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