Vps35 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vps35
Entrez Gene ID	37536
Full Name	Vacuolar protein sorting 35
Synonyms	CG5625,DVps35,DmVps35,Dmel\CG5625,Dvps35,VPS35,dVPS35,dvps35,vps35
General protein information	Preferred Names Vacuolar protein sorting 35 Names CG5625 gene product from transcript CG5625-RB CG5625-PA CG5625-PB Vps35-PA Vps35-PB vacuolar protein sorting 35
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 58C7-58C7

mRNA and Protein(s)

mRNA	Protein	Name
NM_166518.3	NP_726175.3	vacuolar protein sorting 35, isoform B
NM_137807.4	NP_611651.4	vacuolar protein sorting 35, isoform A

Pathways from BioSystems

Homologies

Homo sapiens (human)	VPS35	NP_060676.2
Canis lupus familiaris (dog)	VPS35	XP_532570.3
Eremothecium gossypii	AGOS_AEL107W	NP_984754.1
Arabidopsis thaliana (thale cress)	VPS35B	NP_177713.3
Rattus norvegicus (Norway rat)	Vps35	NP_001099188.2
Danio rerio (zebrafish)	vps35	NP_001020688.2
	VPS35	XP_001108828.2
Gallus gallus (chicken)	VPS35	NP_001005842.1
Caenorhabditis elegans (roundworm)	vps-35	NP_495180.2
Saccharomyces cerevisiae (baker's yeast)	VPS35	NP_012381.1
Kluyveromyces lactis	KLLA0B07535g	XP_451864.1
Magnaporthe oryzae (rice blast fungus)	MGG_05089	XP_003712611.1
Bos taurus (cattle)	VPS35	NP_001039723.1
Drosophila melanogaster (fruit fly)	Vps35	NP_611651.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007683	XP_308188.4
Schizosaccharomyces pombe (fission yeast)	vps35	NP_588260.2
Oryza sativa (rice)	Os03g0801600	NP_001051593.1
Pan troglodytes (chimpanzee)	VPS35	XP_001161536.1
Mus musculus (house mouse)	Vps35	NP_075373.1
Neurospora crassa	NCU06284	XP_962546.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The findings of this study indicate that the VPS35 may play a crucial role in alpha-synucle in degradation by modulating the maturation of cathepsin D and might thereby contribute to the pathogenesis of the disease.
Title: VPS35 dysfunction impairs lysosomal degradation of ?-synuclein and exacerbates neurotoxicity in a Drosophila model of Parkinson's disease.

The in vivo effects of P316S, D620N and L774M of VPS35 using Drosophila as a model, were investigated.
Title: In vivo evidence of pathogenicity of VPS35 mutations in the Drosophila.

overexpression of Vps35 ameliorates the pathogenic mutant LRRK2 eye phenotype in a Drosophila model of Parkinson's disease
Title: Vacuolar protein sorting 35 (Vps35) rescues locomotor deficits and shortened lifespan in Drosophila expressing a Parkinson's disease mutant of Leucine-Rich Repeat Kinase 2 (LRRK2).

Vps35 negatively regulates actin polymerisation, and genetic interactions suggest that some of the endocytic and signalling defects of vps35 mutants are due to this function
Title: Drosophila Vps35 function is necessary for normal endocytic trafficking and actin cytoskeleton organisation.

The following Vps35 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vps35 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10897	NM_166518.3 Latest version!	Drosophila melanogaster vacuolar protein sorting 35, transcript variant B (Vps35), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa10898	NM_137807.4 Latest version!	Drosophila melanogaster vacuolar protein sorting 35, transcript variant A (Vps35), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vps35 ( NM_166518.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726175.3 Drosophila melanogaster vacuolar protein sorting 35, transcript variant B (Vps35), mRNA.

Price & Availability↑

CloneID	OFa10897
Clone ID Related Accession (Same CDS sequence)	NM_166518.3
Accession Version	NM_166518.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar protein sorting 35, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166518.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTATCCCT GGAGCTCCTC CTCGTACGGC GGCCAGATTC CCAGTGCCGC CAACGTCCGG 
ACCATGCCGA ATGGTTTGGA TGACCAGGAG AAGCTGCTCG CTGAAGCCAT TGGACTAGCG 
AGGAAGCAGG CCTTTCAAAT GAACCACTTT CTGGATAAGG AGCGTATGCT GGACTCGCTC 
AAGTGCGCCA GCACCATGCT GGGCGAGCTG CGGACCTCCG TCCTTTCCCC CAAAAGTTAC 
TACGAGCTGT ACATGGCCGT CACCAACGAA CTGTGCCACC TGGAGCTGTA CCTCAGCGAG 
AAAATCGACA AGAAAACGGA TTTGTACGAG CTCGTTCAGT ACTCGCATAC TATTGTGCCG 
CGGTTGTACC TCCTCATAAC AGTGGGCATT GTGTATATCA AGAATGACCC TACGCTGAAG 
CGCAGCATTC TAAAGGACCT GGTCGAGATG TGCCGCGGTG TCCAGCATCC GTTGCGAGGC 
CTCTTTCTGC GCAACTACCT GCTGCAGTGC ACCCGGAACA TCCTGCCCGA TGTGATGGTT 
GAGGAGAACG AGCACGAGGG CAATGTCTAT GATGCCATCG ACTTCGTGCT GACCAACTTT 
GCCGAGATGA ACAAGCTGTG GGTCCGCATG CAGCACCAGG GACACTCCAG CGAAAAGACG 
CGTCGCGAGA AGGAGCGTGA GGAACTAAAG ATCCTGGTAG GCACTAATCT GGTGCGGTTG 
TCCCAGCTGG AGTCGGCCAC TCTGGAAATC TACCAGCGAC TCATTCTACC CGGCATCCTG 
GAGCAGGTGG TCAGCTGTCG CGATGCAATT GCACAGGAGT ACCTAATGGA GTGCATCATC 
CAGGTGTTCC CCGACGAGTT CCACCTAAAG ACACTTGACC CCTTCCTAAA GTCCTGCGCT 
CAGCTGGAGA CCGGTGTTAA TGTGAAGAAC ATCATAATAT CGTTAATCGA ACGATTGGCT 
GCCTATAACC AGCGAAGTGG CAAGACAAGT GGTAATGCCA TCGATGCCAT CATACCCGCC 
GAGGTGGAAC TGTTTGAAGT TTTCAGTGTC CAAGTGGCTA ACATCGTGCA GACCCGAATG 
GACATGCCGT TGGAGGACAC CATATCGCTG CAGGTTGCTC TGCTGAGCTT GGCTCAGAAA 
GTGTATCCCG ATCGAGTTGA CTATGTAGAT AAGGTGCTGG GCACAACCGC TCAAATTTTG 
CAGCGCATGA ACATGAACAA CATCTCCCAT TTGCTATCTG TAAACCAGGA GTTATCGCGC 
CTTCTGCGCA TCTGTATCGA CTTCTACAAC AACGCCTTGA CCATAATTCA ACTTCAAAAC 
TTTTGCCCGC TGCTCGAAAA GTTTGACTAC ACATCCCGTA AATCGCTGGC CCTGTACCTA 
GTTATGAACA TCTTGGACAA CGAAACCCTA GTTCCAACAG CTGATCAGGC GGATAGTCTG 
CTGACCATCA TTACGCCGTT GATTAAGGAC GATGATACGA ACAAAGAAAA CGGCGCGGCA 
GCAGGAAATA CGACTCCGGA TGCCGAGGAG TTTGCCGAAG AGCAGGGGGT TGTGGCACGA 
TTTATTCACC TCATGCGCTC AGATGAACCG GACATGCAGT ATAAAATGCT GCAGACCGCC 
CGAAAGCATC TGGGAAATGG GGGCGGGCAA AGGCTAAAGC ATGTCCTACC TCCGCTTGTC 
TTCGCCGCCT ATCAGCTGGC CTTCAAGTAC AAGGCGATCG CCGAGCAGGA TGAGAACTGG 
GACAAGAAGT GTCAGAAAAT CGTGCAATAC TGCCACAGTA CCATCAGTGC GCTGGCCAAA 
GCCGATCTCG CAGACCTAGC CCTGCGTCTA TACCTGCAAG GTGCCCTGGT CATTGGGGAG 
ATCGGTTATA CGAATCACGA AACGGTGGCA TACGAATTCA TGACCCAGGC CTTCTCCCTC 
TACGAAGACG AGATCTCCGA CTCAAAGGCC CAGCTTGCAG CCATAACGCT CATCATGTCC 
ACTTTTGAAC AAATGTCCTG CTTTGGCGAG GAGAACGCTG AGCCGCTTAG GACGAATTGT 
GCGCTGGCTG CTTCAAAATT GCTGAAAAAA CCGGATCAAT GTCGCGGTGT TGTGGCCTGT 
GCGGCGCTTT TCTGGAGTGG AAAGCAAAAC GGCGAGGAGA TGCGTGACGA GAAGCGAACT 
TTGGACTGCT TAAAGAAGGG TGCTCGCATC GCTTCGCAGT GCTTGGATAC CGGAGTGCAA 
GTTCAATTGT ATGTGGAGCT GCTGAATCAC TATCTGTTTT ACTTCGAACG CGGCAACTCG 
CTTATCACTG TGGCTATGCT CAACCAGCTA ATTGCCAAGG TGAATGAGGA GCTGCCCAAC 
CTGGAGCCCA GCGAGGAGAC CAAACAGATC GAGAGCCATT ACAAGAACAC GCTGGCACAC 
ATCCGCAGTC GCATGGAATC CAATGACTCG TCGCTGGAGG TCTCCTTTGC GGGCATAACT 
CTCAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726175.3
CDS	290..2758
Translation	MYPWSSSSYGGQIPSAANVRTMPNGLDDQEKLLAEAIGLARKQAFQMNHFLDKERMLDSLKCASTMLGELRTSVLSPKSYYELYMAVTNELCHLELYLSEKIDKKTDLYELVQYSHTIVPRLYLLITVGIVYIKNDPTLKRSILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDVMVEENEHEGNVYDAIDFVLTNFAEMNKLWVRMQHQGHSSEKTRREKEREELKILVGTNLVRLSQLESATLEIYQRLILPGILEQVVSCRDAIAQEYLMECIIQVFPDEFHLKTLDPFLKSCAQLETGVNVKNIIISLIERLAAYNQRSGKTSGNAIDAIIPAEVELFEVFSVQVANIVQTRMDMPLEDTISLQVALLSLAQKVYPDRVDYVDKVLGTTAQILQRMNMNNISHLLSVNQELSRLLRICIDFYNNALTIIQLQNFCPLLEKFDYTSRKSLALYLVMNILDNETLVPTADQADSLLTIITPLIKDDDTNKENGAAAGNTTPDAEEFAEEQGVVARFIHLMRSDEPDMQYKMLQTARKHLGNGGGQRLKHVLPPLVFAAYQLAFKYKAIAEQDENWDKKCQKIVQYCHSTISALAKADLADLALRLYLQGALVIGEIGYTNHETVAYEFMTQAFSLYEDEISDSKAQLAAITLIMSTFEQMSCFGEENAEPLRTNCALAASKLLKKPDQCRGVVACAALFWSGKQNGEEMRDEKRTLDCLKKGARIASQCLDTGVQVQLYVELLNHYLFYFERGNSLITVAMLNQLIAKVNEELPNLEPSEETKQIESHYKNTLAHIRSRMESNDSSLEVSFAGITLN

Target ORF information:

RefSeq Version	NM_166518.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar protein sorting 35, transcript variant B (Vps35), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166518.3

ORF Insert Sequence:

ATGTATCCCT GGAGCTCCTC CTCGTACGGC GGCCAGATTC CCAGTGCCGC CAACGTCCGG 
ACCATGCCGA ATGGTTTGGA TGACCAGGAG AAGCTGCTCG CTGAAGCCAT TGGACTAGCG 
AGGAAGCAGG CCTTTCAAAT GAACCACTTT CTGGATAAGG AGCGTATGCT GGACTCGCTC 
AAGTGCGCCA GCACCATGCT GGGCGAGCTG CGGACCTCCG TCCTTTCCCC CAAAAGTTAC 
TACGAGCTGT ACATGGCCGT CACCAACGAA CTGTGCCACC TGGAGCTGTA CCTCAGCGAG 
AAAATCGACA AGAAAACGGA TTTGTACGAG CTCGTTCAGT ACTCGCATAC TATTGTGCCG 
CGGTTGTACC TCCTCATAAC AGTGGGCATT GTGTATATCA AGAATGACCC TACGCTGAAG 
CGCAGCATTC TAAAGGACCT GGTCGAGATG TGCCGCGGTG TCCAGCATCC GTTGCGAGGC 
CTCTTTCTGC GCAACTACCT GCTGCAGTGC ACCCGGAACA TCCTGCCCGA TGTGATGGTT 
GAGGAGAACG AGCACGAGGG CAATGTCTAT GATGCCATCG ACTTCGTGCT GACCAACTTT 
GCCGAGATGA ACAAGCTGTG GGTCCGCATG CAGCACCAGG GACACTCCAG CGAAAAGACG 
CGTCGCGAGA AGGAGCGTGA GGAACTAAAG ATCCTGGTAG GCACTAATCT GGTGCGGTTG 
TCCCAGCTGG AGTCGGCCAC TCTGGAAATC TACCAGCGAC TCATTCTACC CGGCATCCTG 
GAGCAGGTGG TCAGCTGTCG CGATGCAATT GCACAGGAGT ACCTAATGGA GTGCATCATC 
CAGGTGTTCC CCGACGAGTT CCACCTAAAG ACACTTGACC CCTTCCTAAA GTCCTGCGCT 
CAGCTGGAGA CCGGTGTTAA TGTGAAGAAC ATCATAATAT CGTTAATCGA ACGATTGGCT 
GCCTATAACC AGCGAAGTGG CAAGACAAGT GGTAATGCCA TCGATGCCAT CATACCCGCC 
GAGGTGGAAC TGTTTGAAGT TTTCAGTGTC CAAGTGGCTA ACATCGTGCA GACCCGAATG 
GACATGCCGT TGGAGGACAC CATATCGCTG CAGGTTGCTC TGCTGAGCTT GGCTCAGAAA 
GTGTATCCCG ATCGAGTTGA CTATGTAGAT AAGGTGCTGG GCACAACCGC TCAAATTTTG 
CAGCGCATGA ACATGAACAA CATCTCCCAT TTGCTATCTG TAAACCAGGA GTTATCGCGC 
CTTCTGCGCA TCTGTATCGA CTTCTACAAC AACGCCTTGA CCATAATTCA ACTTCAAAAC 
TTTTGCCCGC TGCTCGAAAA GTTTGACTAC ACATCCCGTA AATCGCTGGC CCTGTACCTA 
GTTATGAACA TCTTGGACAA CGAAACCCTA GTTCCAACAG CTGATCAGGC GGATAGTCTG 
CTGACCATCA TTACGCCGTT GATTAAGGAC GATGATACGA ACAAAGAAAA CGGCGCGGCA 
GCAGGAAATA CGACTCCGGA TGCCGAGGAG TTTGCCGAAG AGCAGGGGGT TGTGGCACGA 
TTTATTCACC TCATGCGCTC AGATGAACCG GACATGCAGT ATAAAATGCT GCAGACCGCC 
CGAAAGCATC TGGGAAATGG GGGCGGGCAA AGGCTAAAGC ATGTCCTACC TCCGCTTGTC 
TTCGCCGCCT ATCAGCTGGC CTTCAAGTAC AAGGCGATCG CCGAGCAGGA TGAGAACTGG 
GACAAGAAGT GTCAGAAAAT CGTGCAATAC TGCCACAGTA CCATCAGTGC GCTGGCCAAA 
GCCGATCTCG CAGACCTAGC CCTGCGTCTA TACCTGCAAG GTGCCCTGGT CATTGGGGAG 
ATCGGTTATA CGAATCACGA AACGGTGGCA TACGAATTCA TGACCCAGGC CTTCTCCCTC 
TACGAAGACG AGATCTCCGA CTCAAAGGCC CAGCTTGCAG CCATAACGCT CATCATGTCC 
ACTTTTGAAC AAATGTCCTG CTTTGGCGAG GAGAACGCTG AGCCGCTTAG GACGAATTGT 
GCGCTGGCTG CTTCAAAATT GCTGAAAAAA CCGGATCAAT GTCGCGGTGT TGTGGCCTGT 
GCGGCGCTTT TCTGGAGTGG AAAGCAAAAC GGCGAGGAGA TGCGTGACGA GAAGCGAACT 
TTGGACTGCT TAAAGAAGGG TGCTCGCATC GCTTCGCAGT GCTTGGATAC CGGAGTGCAA 
GTTCAATTGT ATGTGGAGCT GCTGAATCAC TATCTGTTTT ACTTCGAACG CGGCAACTCG 
CTTATCACTG TGGCTATGCT CAACCAGCTA ATTGCCAAGG TGAATGAGGA GCTGCCCAAC 
CTGGAGCCCA GCGAGGAGAC CAAACAGATC GAGAGCCATT ACAAGAACAC GCTGGCACAC 
ATCCGCAGTC GCATGGAATC CAATGACTCG TCGCTGGAGG TCTCCTTTGC GGGCATAACT 
CTCAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vps35 ( NM_137807.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611651.4 Drosophila melanogaster vacuolar protein sorting 35, transcript variant A (Vps35), mRNA.

Price & Availability↑

CloneID	OFa10898
Clone ID Related Accession (Same CDS sequence)	NM_137807.4
Accession Version	NM_137807.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar protein sorting 35, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137807.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCATGC CGAATGGTTT GGATGACCAG GAGAAGCTGC TCGCTGAAGC CATTGGACTA 
GCGAGGAAGC AGGCCTTTCA AATGAACCAC TTTCTGGATA AGGAGCGTAT GCTGGACTCG 
CTCAAGTGCG CCAGCACCAT GCTGGGCGAG CTGCGGACCT CCGTCCTTTC CCCCAAAAGT 
TACTACGAGC TGTACATGGC CGTCACCAAC GAACTGTGCC ACCTGGAGCT GTACCTCAGC 
GAGAAAATCG ACAAGAAAAC GGATTTGTAC GAGCTCGTTC AGTACTCGCA TACTATTGTG 
CCGCGGTTGT ACCTCCTCAT AACAGTGGGC ATTGTGTATA TCAAGAATGA CCCTACGCTG 
AAGCGCAGCA TTCTAAAGGA CCTGGTCGAG ATGTGCCGCG GTGTCCAGCA TCCGTTGCGA 
GGCCTCTTTC TGCGCAACTA CCTGCTGCAG TGCACCCGGA ACATCCTGCC CGATGTGATG 
GTTGAGGAGA ACGAGCACGA GGGCAATGTC TATGATGCCA TCGACTTCGT GCTGACCAAC 
TTTGCCGAGA TGAACAAGCT GTGGGTCCGC ATGCAGCACC AGGGACACTC CAGCGAAAAG 
ACGCGTCGCG AGAAGGAGCG TGAGGAACTA AAGATCCTGG TAGGCACTAA TCTGGTGCGG 
TTGTCCCAGC TGGAGTCGGC CACTCTGGAA ATCTACCAGC GACTCATTCT ACCCGGCATC 
CTGGAGCAGG TGGTCAGCTG TCGCGATGCA ATTGCACAGG AGTACCTAAT GGAGTGCATC 
ATCCAGGTGT TCCCCGACGA GTTCCACCTA AAGACACTTG ACCCCTTCCT AAAGTCCTGC 
GCTCAGCTGG AGACCGGTGT TAATGTGAAG AACATCATAA TATCGTTAAT CGAACGATTG 
GCTGCCTATA ACCAGCGAAG TGGCAAGACA AGTGGTAATG CCATCGATGC CATCATACCC 
GCCGAGGTGG AACTGTTTGA AGTTTTCAGT GTCCAAGTGG CTAACATCGT GCAGACCCGA 
ATGGACATGC CGTTGGAGGA CACCATATCG CTGCAGGTTG CTCTGCTGAG CTTGGCTCAG 
AAAGTGTATC CCGATCGAGT TGACTATGTA GATAAGGTGC TGGGCACAAC CGCTCAAATT 
TTGCAGCGCA TGAACATGAA CAACATCTCC CATTTGCTAT CTGTAAACCA GGAGTTATCG 
CGCCTTCTGC GCATCTGTAT CGACTTCTAC AACAACGCCT TGACCATAAT TCAACTTCAA 
AACTTTTGCC CGCTGCTCGA AAAGTTTGAC TACACATCCC GTAAATCGCT GGCCCTGTAC 
CTAGTTATGA ACATCTTGGA CAACGAAACC CTAGTTCCAA CAGCTGATCA GGCGGATAGT 
CTGCTGACCA TCATTACGCC GTTGATTAAG GACGATGATA CGAACAAAGA AAACGGCGCG 
GCAGCAGGAA ATACGACTCC GGATGCCGAG GAGTTTGCCG AAGAGCAGGG GGTTGTGGCA 
CGATTTATTC ACCTCATGCG CTCAGATGAA CCGGACATGC AGTATAAAAT GCTGCAGACC 
GCCCGAAAGC ATCTGGGAAA TGGGGGCGGG CAAAGGCTAA AGCATGTCCT ACCTCCGCTT 
GTCTTCGCCG CCTATCAGCT GGCCTTCAAG TACAAGGCGA TCGCCGAGCA GGATGAGAAC 
TGGGACAAGA AGTGTCAGAA AATCGTGCAA TACTGCCACA GTACCATCAG TGCGCTGGCC 
AAAGCCGATC TCGCAGACCT AGCCCTGCGT CTATACCTGC AAGGTGCCCT GGTCATTGGG 
GAGATCGGTT ATACGAATCA CGAAACGGTG GCATACGAAT TCATGACCCA GGCCTTCTCC 
CTCTACGAAG ACGAGATCTC CGACTCAAAG GCCCAGCTTG CAGCCATAAC GCTCATCATG 
TCCACTTTTG AACAAATGTC CTGCTTTGGC GAGGAGAACG CTGAGCCGCT TAGGACGAAT 
TGTGCGCTGG CTGCTTCAAA ATTGCTGAAA AAACCGGATC AATGTCGCGG TGTTGTGGCC 
TGTGCGGCGC TTTTCTGGAG TGGAAAGCAA AACGGCGAGG AGATGCGTGA CGAGAAGCGA 
ACTTTGGACT GCTTAAAGAA GGGTGCTCGC ATCGCTTCGC AGTGCTTGGA TACCGGAGTG 
CAAGTTCAAT TGTATGTGGA GCTGCTGAAT CACTATCTGT TTTACTTCGA ACGCGGCAAC 
TCGCTTATCA CTGTGGCTAT GCTCAACCAG CTAATTGCCA AGGTGAATGA GGAGCTGCCC 
AACCTGGAGC CCAGCGAGGA GACCAAACAG ATCGAGAGCC ATTACAAGAA CACGCTGGCA 
CACATCCGCA GTCGCATGGA ATCCAATGAC TCGTCGCTGG AGGTCTCCTT TGCGGGCATA 
ACTCTCAATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611651.4
CDS	121..2532
Translation	MTMPNGLDDQEKLLAEAIGLARKQAFQMNHFLDKERMLDSLKCASTMLGELRTSVLSPKSYYELYMAVTNELCHLELYLSEKIDKKTDLYELVQYSHTIVPRLYLLITVGIVYIKNDPTLKRSILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDVMVEENEHEGNVYDAIDFVLTNFAEMNKLWVRMQHQGHSSEKTRREKEREELKILVGTNLVRLSQLESATLEIYQRLILPGILEQVVSCRDAIAQEYLMECIIQVFPDEFHLKTLDPFLKSCAQLETGVNVKNIIISLIERLAAYNQRSGKTSGNAIDAIIPAEVELFEVFSVQVANIVQTRMDMPLEDTISLQVALLSLAQKVYPDRVDYVDKVLGTTAQILQRMNMNNISHLLSVNQELSRLLRICIDFYNNALTIIQLQNFCPLLEKFDYTSRKSLALYLVMNILDNETLVPTADQADSLLTIITPLIKDDDTNKENGAAAGNTTPDAEEFAEEQGVVARFIHLMRSDEPDMQYKMLQTARKHLGNGGGQRLKHVLPPLVFAAYQLAFKYKAIAEQDENWDKKCQKIVQYCHSTISALAKADLADLALRLYLQGALVIGEIGYTNHETVAYEFMTQAFSLYEDEISDSKAQLAAITLIMSTFEQMSCFGEENAEPLRTNCALAASKLLKKPDQCRGVVACAALFWSGKQNGEEMRDEKRTLDCLKKGARIASQCLDTGVQVQLYVELLNHYLFYFERGNSLITVAMLNQLIAKVNEELPNLEPSEETKQIESHYKNTLAHIRSRMESNDSSLEVSFAGITLN

Target ORF information:

RefSeq Version	NM_137807.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar protein sorting 35, transcript variant A (Vps35), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137807.4

ORF Insert Sequence:

ATGACCATGC CGAATGGTTT GGATGACCAG GAGAAGCTGC TCGCTGAAGC CATTGGACTA 
GCGAGGAAGC AGGCCTTTCA AATGAACCAC TTTCTGGATA AGGAGCGTAT GCTGGACTCG 
CTCAAGTGCG CCAGCACCAT GCTGGGCGAG CTGCGGACCT CCGTCCTTTC CCCCAAAAGT 
TACTACGAGC TGTACATGGC CGTCACCAAC GAACTGTGCC ACCTGGAGCT GTACCTCAGC 
GAGAAAATCG ACAAGAAAAC GGATTTGTAC GAGCTCGTTC AGTACTCGCA TACTATTGTG 
CCGCGGTTGT ACCTCCTCAT AACAGTGGGC ATTGTGTATA TCAAGAATGA CCCTACGCTG 
AAGCGCAGCA TTCTAAAGGA CCTGGTCGAG ATGTGCCGCG GTGTCCAGCA TCCGTTGCGA 
GGCCTCTTTC TGCGCAACTA CCTGCTGCAG TGCACCCGGA ACATCCTGCC CGATGTGATG 
GTTGAGGAGA ACGAGCACGA GGGCAATGTC TATGATGCCA TCGACTTCGT GCTGACCAAC 
TTTGCCGAGA TGAACAAGCT GTGGGTCCGC ATGCAGCACC AGGGACACTC CAGCGAAAAG 
ACGCGTCGCG AGAAGGAGCG TGAGGAACTA AAGATCCTGG TAGGCACTAA TCTGGTGCGG 
TTGTCCCAGC TGGAGTCGGC CACTCTGGAA ATCTACCAGC GACTCATTCT ACCCGGCATC 
CTGGAGCAGG TGGTCAGCTG TCGCGATGCA ATTGCACAGG AGTACCTAAT GGAGTGCATC 
ATCCAGGTGT TCCCCGACGA GTTCCACCTA AAGACACTTG ACCCCTTCCT AAAGTCCTGC 
GCTCAGCTGG AGACCGGTGT TAATGTGAAG AACATCATAA TATCGTTAAT CGAACGATTG 
GCTGCCTATA ACCAGCGAAG TGGCAAGACA AGTGGTAATG CCATCGATGC CATCATACCC 
GCCGAGGTGG AACTGTTTGA AGTTTTCAGT GTCCAAGTGG CTAACATCGT GCAGACCCGA 
ATGGACATGC CGTTGGAGGA CACCATATCG CTGCAGGTTG CTCTGCTGAG CTTGGCTCAG 
AAAGTGTATC CCGATCGAGT TGACTATGTA GATAAGGTGC TGGGCACAAC CGCTCAAATT 
TTGCAGCGCA TGAACATGAA CAACATCTCC CATTTGCTAT CTGTAAACCA GGAGTTATCG 
CGCCTTCTGC GCATCTGTAT CGACTTCTAC AACAACGCCT TGACCATAAT TCAACTTCAA 
AACTTTTGCC CGCTGCTCGA AAAGTTTGAC TACACATCCC GTAAATCGCT GGCCCTGTAC 
CTAGTTATGA ACATCTTGGA CAACGAAACC CTAGTTCCAA CAGCTGATCA GGCGGATAGT 
CTGCTGACCA TCATTACGCC GTTGATTAAG GACGATGATA CGAACAAAGA AAACGGCGCG 
GCAGCAGGAA ATACGACTCC GGATGCCGAG GAGTTTGCCG AAGAGCAGGG GGTTGTGGCA 
CGATTTATTC ACCTCATGCG CTCAGATGAA CCGGACATGC AGTATAAAAT GCTGCAGACC 
GCCCGAAAGC ATCTGGGAAA TGGGGGCGGG CAAAGGCTAA AGCATGTCCT ACCTCCGCTT 
GTCTTCGCCG CCTATCAGCT GGCCTTCAAG TACAAGGCGA TCGCCGAGCA GGATGAGAAC 
TGGGACAAGA AGTGTCAGAA AATCGTGCAA TACTGCCACA GTACCATCAG TGCGCTGGCC 
AAAGCCGATC TCGCAGACCT AGCCCTGCGT CTATACCTGC AAGGTGCCCT GGTCATTGGG 
GAGATCGGTT ATACGAATCA CGAAACGGTG GCATACGAAT TCATGACCCA GGCCTTCTCC 
CTCTACGAAG ACGAGATCTC CGACTCAAAG GCCCAGCTTG CAGCCATAAC GCTCATCATG 
TCCACTTTTG AACAAATGTC CTGCTTTGGC GAGGAGAACG CTGAGCCGCT TAGGACGAAT 
TGTGCGCTGG CTGCTTCAAA ATTGCTGAAA AAACCGGATC AATGTCGCGG TGTTGTGGCC 
TGTGCGGCGC TTTTCTGGAG TGGAAAGCAA AACGGCGAGG AGATGCGTGA CGAGAAGCGA 
ACTTTGGACT GCTTAAAGAA GGGTGCTCGC ATCGCTTCGC AGTGCTTGGA TACCGGAGTG 
CAAGTTCAAT TGTATGTGGA GCTGCTGAAT CACTATCTGT TTTACTTCGA ACGCGGCAAC 
TCGCTTATCA CTGTGGCTAT GCTCAACCAG CTAATTGCCA AGGTGAATGA GGAGCTGCCC 
AACCTGGAGC CCAGCGAGGA GACCAAACAG ATCGAGAGCC ATTACAAGAA CACGCTGGCA 
CACATCCGCA GTCGCATGGA ATCCAATGAC TCGTCGCTGG AGGTCTCCTT TGCGGGCATA 
ACTCTCAATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Vps35 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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