CG10543 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG10543
Entrez Gene ID	37379
Full Name	CG10543 gene product from transcript CG10543-RF
Synonyms	Dmel\CG10543
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 57B5-57B5

mRNA and Protein(s)

mRNA	Protein	Name
NM_137690.4	NP_611534.2	uncharacterized protein, isoform A
NM_001274183.1	NP_001261112.1	uncharacterized protein, isoform F
NM_176241.3	NP_788421.2	uncharacterized protein, isoform D
NM_166416.2	NP_726015.2	uncharacterized protein, isoform E

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG10543 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG10543 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa10730	NM_137690.4 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG10543), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa10730	NM_166416.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant E (CG10543), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa10731	NM_176241.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant D (CG10543), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa12093	NM_001274183.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant F (CG10543), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG10543 ( NM_137690.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611534.2 Drosophila melanogaster uncharacterized protein, transcript variant A (CG10543), mRNA.

Price & Availability↑

CloneID	OFa10730
Clone ID Related Accession (Same CDS sequence)	NM_137690.4 , NM_166416.2
Accession Version	NM_137690.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_137690.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATAACA AAACTACACA GTTGGATTTT AAATTACTGG TGGATCAATC ACTAAATCTA 
CTGGACAGCC TCAACACGGC ATTGCGGTGC GATGCTATTC AATTCTTGCT GCAGACGCTT 
AAGTGCAAAT ACCCGGAGTC CTGCGACCAA GTCGTCAGGA ATCTGTTCAG TCACATAGCT 
GCGGCCAATG ACAATGGCGA GTCTGGAGGC AGGGCGCAGC AGCACGAGCT AGCGAGGACG 
AAGCAGCTCC AGCTGCTGCT GACCGCCAAA AAGGAGAAGA AAGAGCTGGG AATCGGTTGC 
GAGGCGGAGA TCAAGCGAGA GAACGATGAG GAGGAGGCGG GCTCGACGGA TCTGAACCAA 
AATTTCGAGA AGATACCCTG CAAAGAGGAG TTCCTGTGCC TCGAGGAGGA AGAGGAGGAC 
TTTCTCGACG ATGCGGACAC GCAGAACTCG TCTTCCCTGC AGTTCCACAC CGGTAACAAT 
GTGGACGCCC TGGCCCTGGG CCCTGGCGAT CCGCTGGAGC TGATCCAGTC CGGAGTTTCG 
CTGCTGGTCA AGCGGAAGTA TGCTGCCACC TTCGATGACG AACTGATCGG GGATGGGGAT 
GGTGATGAGG CCAACAGTAA CAGCAGTGAC GGCAAGATGG TCAAGCGAAA GCGCACCAAC 
AACATGCACC TGACCGTGTC GAGTGTGCGA CGGAAAGAGG AGCACGGCCA GCTGGGCGAT 
GGCTATGTCT TCCACGAGGA CAGCCAGTAT ACGATGCGAT ATCACCACAG CACCGGCGAG 
TTCAGTGAGC GGGCCTTTAA GGCCCAGTTC CGGGCTCTGC TCCGCCTGGC GCTCAGTCAG 
CACCACAAGC CCTTTCTCCG CCAGTTCTAC GAGAACTTTG TTGTGAATGG ACGCTTGCTG 
GGGACCACGC CCTCGATGCG TGTCCTGAAG GAGCTGCGCG AACAGCGGCT GGAGGAGTGG 
CGACGTCAGC GTGAACGCCG CCAACTGGTC ATCCTGATGG AGCAGAGCGA GACTCAGCGG 
GACGAAGAGT TTCAGCAGCA GCAGGAGCTG GTGGAGAACC AAGAGGAGGT CCAGCAGGAG 
CAAGAACAGC AACAGCAGCA GCTGGAGGAT ATTCAGCAGC AGCATCTGGA GGAGATTCAG 
CGGCAGCAGC AATTGCCGGC CATCTCGTTT GGCGACTCCG AATTGGAGTC AGAAACGGAA 
TCGCAGCTGT CGTCGGCTGC CCAAGAGATT ATGAAGTTTG AGGAGTTCAT TGACGAGGGT 
GTAGGCGCTG GCCGTCTGAT CGATGGTCTG GAGATGGAAC CCGAGATCGA TGACATGCTA 
GCTGGCGCCG GCAGTATGAA CAGCGCCAGT GGACACAGCA GCAACAGTAG CAGCAGCGGA 
AGTGGGAGCG CCGGTAATGG GAATGGCATC AGCGGGGTGA TCCTGGAGAA CAATAGTCAT 
CATACGCATC ACCTAAGTAG CAGCAGCAGC GCAAATCTTG TGATCAACGG CCTTACGTCA 
AGCAATAGCA TCAGCAACAA TAATAATAAC AGCAACAATG TCAGTTTACA CCGCCCTCAA 
TTGACGCCAC AGGCGCAGAA TCAGCTGGCA GCGTCGATGA AACCATCAGA CCATATTCCC 
ATATCGATGC CCATGGAGAA CATGGATTTG AAAGCCGGGC AGGCGGCTCA TGGGACTGCC 
AACGCCATCA TGCAGGGCGG TAGTTCGAGC CTCGCGAGCC AGCTACATCA GCAGCAAAAA 
CCCTACAACT CAAGCAATAA TCCGCTGTCT ATGATGGGTG GAATGATGCA GCAGCAGCAG 
CAGCAGCATG TTGCTATGCC GCACCACCAG CGCCAGATGA TGATGATGCC AGAGGATTTC 
CCGCAACACG GAGCCTCAAT GATGAACAGT CGTCAGATGC CCGCTCTGGC CGCCTTGTCG 
AATCTGGGCG ACACACCGGC TATGAGTGGC CAGCTTAACT CCTCCCTAGA GCTGGACGAC 
AATGACCTGT CGGCGGATGA GGACGACGAC GATCTGGACC ATGACCTGGA CGAATTAGAC 
GCGGCCAAGC AACAACTAAT AGATGGCGGC AGCAGTAGCA GCACATCGCT TCAGGCGCCA 
CCATCGCAGC ACGGCAGCGG TAGTGGAGGC AGCGGCGGCC AGACGCCCGG TAGCAAAAAG 
GATAAGCCCA GCTACAACTG CCTGCTCTGC CCCAAGTCGT ATCGCAAGCG CAAGTCCTTG 
CTAGACCACT ACAAAATGCA TCCGGGCTAC TGCCATGACT GCGGTCAGCG CAATGGGAAC 
ACGCTGGAGG AAATAATCCA CCACAACCGG ACAATGCATG TCAAGGAGTT TCCGTTTGTG 
TGCGAGACGT GCGGAGAGTC GTACTCACGC AAACAGCAGT TCCACGCCCA CGTGGAGTCG 
CACAATAAGA AGGAAATCAA AACCTTTCCC TGCGGCGAGT GCGGTCTTAA GTTTCCGCAA 
AAGAAACTGC AGCAACACTT CGAGGAGACG GGCCACAAGG CGGACGGCGC TATCTGCGAG 
GTGTGCGGCG AGGAGTTCCA GTCGAAGAAT GCCCTGTACC AGCACATTAT TCGTGTGCAC 
AAGCGGGACA ACTTCTTCGA GTGCCACATT TGCCATAACC GCTTCACGCT GAAAGCCAAC 
CTGGAGCGGC ACGTCCAGCT GCACACCGAG ATCAAACGGC CCTACGTGTG CGATCTGTGC 
GGCTCGTCCT ACTTCACATA TCCCGCGCTC AAGGAGCACT ACAGCAACGC CCACGTGGAC 
GTGTCCGAGT GCAAATGCAC GCTTTGCGGC AAGCGCTTCG GTTCGGCCAA GTCGCTGCAG 
CGACACCTTC CGTCGCACTC GGAGGAAAGG CCGCACTGCT GCAACTATTG CGATCAGACC 
TTCAAGTGGA AAACGCATCT GGTGCGCCAC AAGCAGACAA TGCACGGAAA CGAGCCGCCT 
CCGCCTAAGA AGGGAAAGCA GCGCTTTCCC AAAACAAGCG AAGAAGATAT GGGTAGCTTA 
CCGGACATGC CGGGTCCACC GCCTGTGAAG GCCAGCAAGA AGGCAGCAAC CAGCAAGGCG 
AAAGCGCAAG CAGCGGCCGC CGCAGCCGCA GCAGCATCAT CCCAGCAACA ACAACAGAAG 
GGTTCTGCAG CAACACCGAC GCCGCCACCG CCGGTCTCCA CCACACCGGG ATGCCTGCAA 
CAGGATCCGT TCAATGCGGC CATGGTTAGC AGCAACAGCT CGCAGTCCTC CACGGCGTCC 
ACGTCGCAAC ACTCGGTGTC GACGAGTGAA TCTCAGCAGA ATTCCATATA CAATCAGAGC 
TTTAATGCGG AGAAACAGCA GCCAGGACAG CAGCAGCCCC AGAATACCTT GCATCAGCAA 
CATCCAACGC CGCCACCTCC GCAGCAGCAG CAACAACAAC AGCAGCAGCA GCAGGCGCTG 
CCACAGTCAC GAGTTGGGCC CGGAGAACTG CATCTGCAGC GGATGAATAG TTTCGGCCAG 
GACGGACAGT TTCACTTTGA GTCCAATCCG GCAGCTGGTG CGTATCAACA GCATCAACTG 
ATCAGCCAGT ATCAGCAACA ACAGCAGCAG CAGCAGCAAC AACAGCAGCA GCAGCCCCAG 
CAGCAGCAAC AACATCATCT CGCCCAACAG CAGCAGCAGC ACATGAGGAG TCACACGCCC 
CAGAGTCCAC ATCCTCCGCA TCCTTCGCAG CTGCAACAAC AGCAGCCCCA ACAGCACTTT 
AGCTCTCCGC ACCACATGCC GCCAATGCAC CATCAACACC AGCTGATGAT GCAACAGCAA 
CATCGCCACA ACCAGCGCTC GCCGCTTCAC CATCATCCTG CGGCGCAGCA GCTTCAGCAG 
CAGCATCAGC AACCATCGCA TTCCCCACAG CATCAGCATG CGAGACTGCA GTCGCCACAA 
CCTGCTCCAG TACAACAACA ACAGCAGCAG CAGCAGCAAC AACAGCAGCA GCAGCAGCAG 
TTCCTGCAGC AACAGGCTGC CGACACGTGG GGCAACATGA ACTTTGGCAA TCCACCGAAC 
AATCAGCAGG TTGAGCTCAA GGATAACAAA TTCTATATTG TGGAATCGGC CGAGTTCCTA 
GGTCTTCCAA TGAATGTGCC GCCATCTCCG CAGCAGCAGC AACAGCAAGC AGTAAGCAAA 
CCCCAACAGC AGCAGCAACA ACAACACCCA CAACCGCAAC AGCCAGATCC AACCCTTGCT 
GGTGATTTGA TGAGCTTCCA GCATATGTGG CCGGCGCCTG GTTTTAGCCA GACTTCACAG 
CAGCAACAGC CACAGCAGCA ACAACAGCAG CAAGCGGCGC AGCAGCAACA GCAGGCGCAA 
AGTCAGGCAA ACTCAAGTGA TCCCCACAAC TACAACAACA TCGGCAGCAT ACTAACGAAT 
CTCATTGACA CAAATCCCGC GCCAATGGAG TACAACTTCG ATCTGATGCA GCAGCAACAA 
ACGCCGTCAG CCCAACAGCA GCAGCAGCAA CAACAACAAC AGCAGGCGGC GCAGCAGCAA 
CATCACAGTG CCGGTGCATA TGGGAGCGGT TTGATGCGCA GCGGAGGCGG CGTTTACGGA 
GGCGCCTACG AGCAGCACTT GAGTGACCAG CTGGTCAGCG AGCAGCAGAA ACAGAACAGC 
TTTCAACCCT ACCACCCACA TGGCCTGCAG GCGCAGCAAC AGCAGCCACT GCATCCGCAT 
CCGCATTTGT TACCCCCGCC AGCGCCGCAT AATAATGTCT ACGATCGCAC GGGAGTTGGA 
GTCGGTGTCG GAGTGGGCGT AGGCGTGGGA GTGGGTGTGG GCTATCCAAT GCTGGGAGAC 
GATACCAAGA GCGTCCTGCC GCCCATGATG GGCGGCATGA TGCAGCAAAT GCCACCGCAC 
GGGCAGCAGC CGGATCTAAT CTACTACCCA GTGAAGAACG ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611534.2
CDS	671..5575
Translation	MDNKTTQLDFKLLVDQSLNLLDSLNTALRCDAIQFLLQTLKCKYPESCDQVVRNLFSHIAAANDNGESGGRAQQHELARTKQLQLLLTAKKEKKELGIGCEAEIKRENDEEEAGSTDLNQNFEKIPCKEEFLCLEEEEEDFLDDADTQNSSSLQFHTGNNVDALALGPGDPLELIQSGVSLLVKRKYAATFDDELIGDGDGDEANSNSSDGKMVKRKRTNNMHLTVSSVRRKEEHGQLGDGYVFHEDSQYTMRYHHSTGEFSERAFKAQFRALLRLALSQHHKPFLRQFYENFVVNGRLLGTTPSMRVLKELREQRLEEWRRQRERRQLVILMEQSETQRDEEFQQQQELVENQEEVQQEQEQQQQQLEDIQQQHLEEIQRQQQLPAISFGDSELESETESQLSSAAQEIMKFEEFIDEGVGAGRLIDGLEMEPEIDDMLAGAGSMNSASGHSSNSSSSGSGSAGNGNGISGVILENNSHHTHHLSSSSSANLVINGLTSSNSISNNNNNSNNVSLHRPQLTPQAQNQLAASMKPSDHIPISMPMENMDLKAGQAAHGTANAIMQGGSSSLASQLHQQQKPYNSSNNPLSMMGGMMQQQQQQHVAMPHHQRQMMMMPEDFPQHGASMMNSRQMPALAALSNLGDTPAMSGQLNSSLELDDNDLSADEDDDDLDHDLDELDAAKQQLIDGGSSSSTSLQAPPSQHGSGSGGSGGQTPGSKKDKPSYNCLLCPKSYRKRKSLLDHYKMHPGYCHDCGQRNGNTLEEIIHHNRTMHVKEFPFVCETCGESYSRKQQFHAHVESHNKKEIKTFPCGECGLKFPQKKLQQHFEETGHKADGAICEVCGEEFQSKNALYQHIIRVHKRDNFFECHICHNRFTLKANLERHVQLHTEIKRPYVCDLCGSSYFTYPALKEHYSNAHVDVSECKCTLCGKRFGSAKSLQRHLPSHSEERPHCCNYCDQTFKWKTHLVRHKQTMHGNEPPPPKKGKQRFPKTSEEDMGSLPDMPGPPPVKASKKAATSKAKAQAAAAAAAAASSQQQQQKGSAATPTPPPPVSTTPGCLQQDPFNAAMVSSNSSQSSTASTSQHSVSTSESQQNSIYNQSFNAEKQQPGQQQPQNTLHQQHPTPPPPQQQQQQQQQQQALPQSRVGPGELHLQRMNSFGQDGQFHFESNPAAGAYQQHQLISQYQQQQQQQQQQQQQQPQQQQQHHLAQQQQQHMRSHTPQSPHPPHPSQLQQQQPQQHFSSPHHMPPMHHQHQLMMQQQHRHNQRSPLHHHPAAQQLQQQHQQPSHSPQHQHARLQSPQPAPVQQQQQQQQQQQQQQQQFLQQQAADTWGNMNFGNPPNNQQVELKDNKFYIVESAEFLGLPMNVPPSPQQQQQQAVSKPQQQQQQQHPQPQQPDPTLAGDLMSFQHMWPAPGFSQTSQQQQPQQQQQQQAAQQQQQAQSQANSSDPHNYNNIGSILTNLIDTNPAPMEYNFDLMQQQQTPSAQQQQQQQQQQQAAQQQHHSAGAYGSGLMRSGGGVYGGAYEQHLSDQLVSEQQKQNSFQPYHPHGLQAQQQQPLHPHPHLLPPPAPHNNVYDRTGVGVGVGVGVGVGVGVGYPMLGDDTKSVLPPMMGGMMQQMPPHGQQPDLIYYPVKND

Target ORF information:

RefSeq Version	NM_137690.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG10543), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137690.4

ORF Insert Sequence:

ATGGATAACA AAACTACACA GTTGGATTTT AAATTACTGG TGGATCAATC ACTAAATCTA 
CTGGACAGCC TCAACACGGC ATTGCGGTGC GATGCTATTC AATTCTTGCT GCAGACGCTT 
AAGTGCAAAT ACCCGGAGTC CTGCGACCAA GTCGTCAGGA ATCTGTTCAG TCACATAGCT 
GCGGCCAATG ACAATGGCGA GTCTGGAGGC AGGGCGCAGC AGCACGAGCT AGCGAGGACG 
AAGCAGCTCC AGCTGCTGCT GACCGCCAAA AAGGAGAAGA AAGAGCTGGG AATCGGTTGC 
GAGGCGGAGA TCAAGCGAGA GAACGATGAG GAGGAGGCGG GCTCGACGGA TCTGAACCAA 
AATTTCGAGA AGATACCCTG CAAAGAGGAG TTCCTGTGCC TCGAGGAGGA AGAGGAGGAC 
TTTCTCGACG ATGCGGACAC GCAGAACTCG TCTTCCCTGC AGTTCCACAC CGGTAACAAT 
GTGGACGCCC TGGCCCTGGG CCCTGGCGAT CCGCTGGAGC TGATCCAGTC CGGAGTTTCG 
CTGCTGGTCA AGCGGAAGTA TGCTGCCACC TTCGATGACG AACTGATCGG GGATGGGGAT 
GGTGATGAGG CCAACAGTAA CAGCAGTGAC GGCAAGATGG TCAAGCGAAA GCGCACCAAC 
AACATGCACC TGACCGTGTC GAGTGTGCGA CGGAAAGAGG AGCACGGCCA GCTGGGCGAT 
GGCTATGTCT TCCACGAGGA CAGCCAGTAT ACGATGCGAT ATCACCACAG CACCGGCGAG 
TTCAGTGAGC GGGCCTTTAA GGCCCAGTTC CGGGCTCTGC TCCGCCTGGC GCTCAGTCAG 
CACCACAAGC CCTTTCTCCG CCAGTTCTAC GAGAACTTTG TTGTGAATGG ACGCTTGCTG 
GGGACCACGC CCTCGATGCG TGTCCTGAAG GAGCTGCGCG AACAGCGGCT GGAGGAGTGG 
CGACGTCAGC GTGAACGCCG CCAACTGGTC ATCCTGATGG AGCAGAGCGA GACTCAGCGG 
GACGAAGAGT TTCAGCAGCA GCAGGAGCTG GTGGAGAACC AAGAGGAGGT CCAGCAGGAG 
CAAGAACAGC AACAGCAGCA GCTGGAGGAT ATTCAGCAGC AGCATCTGGA GGAGATTCAG 
CGGCAGCAGC AATTGCCGGC CATCTCGTTT GGCGACTCCG AATTGGAGTC AGAAACGGAA 
TCGCAGCTGT CGTCGGCTGC CCAAGAGATT ATGAAGTTTG AGGAGTTCAT TGACGAGGGT 
GTAGGCGCTG GCCGTCTGAT CGATGGTCTG GAGATGGAAC CCGAGATCGA TGACATGCTA 
GCTGGCGCCG GCAGTATGAA CAGCGCCAGT GGACACAGCA GCAACAGTAG CAGCAGCGGA 
AGTGGGAGCG CCGGTAATGG GAATGGCATC AGCGGGGTGA TCCTGGAGAA CAATAGTCAT 
CATACGCATC ACCTAAGTAG CAGCAGCAGC GCAAATCTTG TGATCAACGG CCTTACGTCA 
AGCAATAGCA TCAGCAACAA TAATAATAAC AGCAACAATG TCAGTTTACA CCGCCCTCAA 
TTGACGCCAC AGGCGCAGAA TCAGCTGGCA GCGTCGATGA AACCATCAGA CCATATTCCC 
ATATCGATGC CCATGGAGAA CATGGATTTG AAAGCCGGGC AGGCGGCTCA TGGGACTGCC 
AACGCCATCA TGCAGGGCGG TAGTTCGAGC CTCGCGAGCC AGCTACATCA GCAGCAAAAA 
CCCTACAACT CAAGCAATAA TCCGCTGTCT ATGATGGGTG GAATGATGCA GCAGCAGCAG 
CAGCAGCATG TTGCTATGCC GCACCACCAG CGCCAGATGA TGATGATGCC AGAGGATTTC 
CCGCAACACG GAGCCTCAAT GATGAACAGT CGTCAGATGC CCGCTCTGGC CGCCTTGTCG 
AATCTGGGCG ACACACCGGC TATGAGTGGC CAGCTTAACT CCTCCCTAGA GCTGGACGAC 
AATGACCTGT CGGCGGATGA GGACGACGAC GATCTGGACC ATGACCTGGA CGAATTAGAC 
GCGGCCAAGC AACAACTAAT AGATGGCGGC AGCAGTAGCA GCACATCGCT TCAGGCGCCA 
CCATCGCAGC ACGGCAGCGG TAGTGGAGGC AGCGGCGGCC AGACGCCCGG TAGCAAAAAG 
GATAAGCCCA GCTACAACTG CCTGCTCTGC CCCAAGTCGT ATCGCAAGCG CAAGTCCTTG 
CTAGACCACT ACAAAATGCA TCCGGGCTAC TGCCATGACT GCGGTCAGCG CAATGGGAAC 
ACGCTGGAGG AAATAATCCA CCACAACCGG ACAATGCATG TCAAGGAGTT TCCGTTTGTG 
TGCGAGACGT GCGGAGAGTC GTACTCACGC AAACAGCAGT TCCACGCCCA CGTGGAGTCG 
CACAATAAGA AGGAAATCAA AACCTTTCCC TGCGGCGAGT GCGGTCTTAA GTTTCCGCAA 
AAGAAACTGC AGCAACACTT CGAGGAGACG GGCCACAAGG CGGACGGCGC TATCTGCGAG 
GTGTGCGGCG AGGAGTTCCA GTCGAAGAAT GCCCTGTACC AGCACATTAT TCGTGTGCAC 
AAGCGGGACA ACTTCTTCGA GTGCCACATT TGCCATAACC GCTTCACGCT GAAAGCCAAC 
CTGGAGCGGC ACGTCCAGCT GCACACCGAG ATCAAACGGC CCTACGTGTG CGATCTGTGC 
GGCTCGTCCT ACTTCACATA TCCCGCGCTC AAGGAGCACT ACAGCAACGC CCACGTGGAC 
GTGTCCGAGT GCAAATGCAC GCTTTGCGGC AAGCGCTTCG GTTCGGCCAA GTCGCTGCAG 
CGACACCTTC CGTCGCACTC GGAGGAAAGG CCGCACTGCT GCAACTATTG CGATCAGACC 
TTCAAGTGGA AAACGCATCT GGTGCGCCAC AAGCAGACAA TGCACGGAAA CGAGCCGCCT 
CCGCCTAAGA AGGGAAAGCA GCGCTTTCCC AAAACAAGCG AAGAAGATAT GGGTAGCTTA 
CCGGACATGC CGGGTCCACC GCCTGTGAAG GCCAGCAAGA AGGCAGCAAC CAGCAAGGCG 
AAAGCGCAAG CAGCGGCCGC CGCAGCCGCA GCAGCATCAT CCCAGCAACA ACAACAGAAG 
GGTTCTGCAG CAACACCGAC GCCGCCACCG CCGGTCTCCA CCACACCGGG ATGCCTGCAA 
CAGGATCCGT TCAATGCGGC CATGGTTAGC AGCAACAGCT CGCAGTCCTC CACGGCGTCC 
ACGTCGCAAC ACTCGGTGTC GACGAGTGAA TCTCAGCAGA ATTCCATATA CAATCAGAGC 
TTTAATGCGG AGAAACAGCA GCCAGGACAG CAGCAGCCCC AGAATACCTT GCATCAGCAA 
CATCCAACGC CGCCACCTCC GCAGCAGCAG CAACAACAAC AGCAGCAGCA GCAGGCGCTG 
CCACAGTCAC GAGTTGGGCC CGGAGAACTG CATCTGCAGC GGATGAATAG TTTCGGCCAG 
GACGGACAGT TTCACTTTGA GTCCAATCCG GCAGCTGGTG CGTATCAACA GCATCAACTG 
ATCAGCCAGT ATCAGCAACA ACAGCAGCAG CAGCAGCAAC AACAGCAGCA GCAGCCCCAG 
CAGCAGCAAC AACATCATCT CGCCCAACAG CAGCAGCAGC ACATGAGGAG TCACACGCCC 
CAGAGTCCAC ATCCTCCGCA TCCTTCGCAG CTGCAACAAC AGCAGCCCCA ACAGCACTTT 
AGCTCTCCGC ACCACATGCC GCCAATGCAC CATCAACACC AGCTGATGAT GCAACAGCAA 
CATCGCCACA ACCAGCGCTC GCCGCTTCAC CATCATCCTG CGGCGCAGCA GCTTCAGCAG 
CAGCATCAGC AACCATCGCA TTCCCCACAG CATCAGCATG CGAGACTGCA GTCGCCACAA 
CCTGCTCCAG TACAACAACA ACAGCAGCAG CAGCAGCAAC AACAGCAGCA GCAGCAGCAG 
TTCCTGCAGC AACAGGCTGC CGACACGTGG GGCAACATGA ACTTTGGCAA TCCACCGAAC 
AATCAGCAGG TTGAGCTCAA GGATAACAAA TTCTATATTG TGGAATCGGC CGAGTTCCTA 
GGTCTTCCAA TGAATGTGCC GCCATCTCCG CAGCAGCAGC AACAGCAAGC AGTAAGCAAA 
CCCCAACAGC AGCAGCAACA ACAACACCCA CAACCGCAAC AGCCAGATCC AACCCTTGCT 
GGTGATTTGA TGAGCTTCCA GCATATGTGG CCGGCGCCTG GTTTTAGCCA GACTTCACAG 
CAGCAACAGC CACAGCAGCA ACAACAGCAG CAAGCGGCGC AGCAGCAACA GCAGGCGCAA 
AGTCAGGCAA ACTCAAGTGA TCCCCACAAC TACAACAACA TCGGCAGCAT ACTAACGAAT 
CTCATTGACA CAAATCCCGC GCCAATGGAG TACAACTTCG ATCTGATGCA GCAGCAACAA 
ACGCCGTCAG CCCAACAGCA GCAGCAGCAA CAACAACAAC AGCAGGCGGC GCAGCAGCAA 
CATCACAGTG CCGGTGCATA TGGGAGCGGT TTGATGCGCA GCGGAGGCGG CGTTTACGGA 
GGCGCCTACG AGCAGCACTT GAGTGACCAG CTGGTCAGCG AGCAGCAGAA ACAGAACAGC 
TTTCAACCCT ACCACCCACA TGGCCTGCAG GCGCAGCAAC AGCAGCCACT GCATCCGCAT 
CCGCATTTGT TACCCCCGCC AGCGCCGCAT AATAATGTCT ACGATCGCAC GGGAGTTGGA 
GTCGGTGTCG GAGTGGGCGT AGGCGTGGGA GTGGGTGTGG GCTATCCAAT GCTGGGAGAC 
GATACCAAGA GCGTCCTGCC GCCCATGATG GGCGGCATGA TGCAGCAAAT GCCACCGCAC 
GGGCAGCAGC CGGATCTAAT CTACTACCCA GTGAAGAACG ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG10543 ( NM_166416.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726015.2 Drosophila melanogaster uncharacterized protein, transcript variant E (CG10543), mRNA.

Price & Availability↑

CloneID	OFa10730
Clone ID Related Accession (Same CDS sequence)	NM_137690.4 , NM_166416.2
Accession Version	NM_166416.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_166416.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATAACA AAACTACACA GTTGGATTTT AAATTACTGG TGGATCAATC ACTAAATCTA 
CTGGACAGCC TCAACACGGC ATTGCGGTGC GATGCTATTC AATTCTTGCT GCAGACGCTT 
AAGTGCAAAT ACCCGGAGTC CTGCGACCAA GTCGTCAGGA ATCTGTTCAG TCACATAGCT 
GCGGCCAATG ACAATGGCGA GTCTGGAGGC AGGGCGCAGC AGCACGAGCT AGCGAGGACG 
AAGCAGCTCC AGCTGCTGCT GACCGCCAAA AAGGAGAAGA AAGAGCTGGG AATCGGTTGC 
GAGGCGGAGA TCAAGCGAGA GAACGATGAG GAGGAGGCGG GCTCGACGGA TCTGAACCAA 
AATTTCGAGA AGATACCCTG CAAAGAGGAG TTCCTGTGCC TCGAGGAGGA AGAGGAGGAC 
TTTCTCGACG ATGCGGACAC GCAGAACTCG TCTTCCCTGC AGTTCCACAC CGGTAACAAT 
GTGGACGCCC TGGCCCTGGG CCCTGGCGAT CCGCTGGAGC TGATCCAGTC CGGAGTTTCG 
CTGCTGGTCA AGCGGAAGTA TGCTGCCACC TTCGATGACG AACTGATCGG GGATGGGGAT 
GGTGATGAGG CCAACAGTAA CAGCAGTGAC GGCAAGATGG TCAAGCGAAA GCGCACCAAC 
AACATGCACC TGACCGTGTC GAGTGTGCGA CGGAAAGAGG AGCACGGCCA GCTGGGCGAT 
GGCTATGTCT TCCACGAGGA CAGCCAGTAT ACGATGCGAT ATCACCACAG CACCGGCGAG 
TTCAGTGAGC GGGCCTTTAA GGCCCAGTTC CGGGCTCTGC TCCGCCTGGC GCTCAGTCAG 
CACCACAAGC CCTTTCTCCG CCAGTTCTAC GAGAACTTTG TTGTGAATGG ACGCTTGCTG 
GGGACCACGC CCTCGATGCG TGTCCTGAAG GAGCTGCGCG AACAGCGGCT GGAGGAGTGG 
CGACGTCAGC GTGAACGCCG CCAACTGGTC ATCCTGATGG AGCAGAGCGA GACTCAGCGG 
GACGAAGAGT TTCAGCAGCA GCAGGAGCTG GTGGAGAACC AAGAGGAGGT CCAGCAGGAG 
CAAGAACAGC AACAGCAGCA GCTGGAGGAT ATTCAGCAGC AGCATCTGGA GGAGATTCAG 
CGGCAGCAGC AATTGCCGGC CATCTCGTTT GGCGACTCCG AATTGGAGTC AGAAACGGAA 
TCGCAGCTGT CGTCGGCTGC CCAAGAGATT ATGAAGTTTG AGGAGTTCAT TGACGAGGGT 
GTAGGCGCTG GCCGTCTGAT CGATGGTCTG GAGATGGAAC CCGAGATCGA TGACATGCTA 
GCTGGCGCCG GCAGTATGAA CAGCGCCAGT GGACACAGCA GCAACAGTAG CAGCAGCGGA 
AGTGGGAGCG CCGGTAATGG GAATGGCATC AGCGGGGTGA TCCTGGAGAA CAATAGTCAT 
CATACGCATC ACCTAAGTAG CAGCAGCAGC GCAAATCTTG TGATCAACGG CCTTACGTCA 
AGCAATAGCA TCAGCAACAA TAATAATAAC AGCAACAATG TCAGTTTACA CCGCCCTCAA 
TTGACGCCAC AGGCGCAGAA TCAGCTGGCA GCGTCGATGA AACCATCAGA CCATATTCCC 
ATATCGATGC CCATGGAGAA CATGGATTTG AAAGCCGGGC AGGCGGCTCA TGGGACTGCC 
AACGCCATCA TGCAGGGCGG TAGTTCGAGC CTCGCGAGCC AGCTACATCA GCAGCAAAAA 
CCCTACAACT CAAGCAATAA TCCGCTGTCT ATGATGGGTG GAATGATGCA GCAGCAGCAG 
CAGCAGCATG TTGCTATGCC GCACCACCAG CGCCAGATGA TGATGATGCC AGAGGATTTC 
CCGCAACACG GAGCCTCAAT GATGAACAGT CGTCAGATGC CCGCTCTGGC CGCCTTGTCG 
AATCTGGGCG ACACACCGGC TATGAGTGGC CAGCTTAACT CCTCCCTAGA GCTGGACGAC 
AATGACCTGT CGGCGGATGA GGACGACGAC GATCTGGACC ATGACCTGGA CGAATTAGAC 
GCGGCCAAGC AACAACTAAT AGATGGCGGC AGCAGTAGCA GCACATCGCT TCAGGCGCCA 
CCATCGCAGC ACGGCAGCGG TAGTGGAGGC AGCGGCGGCC AGACGCCCGG TAGCAAAAAG 
GATAAGCCCA GCTACAACTG CCTGCTCTGC CCCAAGTCGT ATCGCAAGCG CAAGTCCTTG 
CTAGACCACT ACAAAATGCA TCCGGGCTAC TGCCATGACT GCGGTCAGCG CAATGGGAAC 
ACGCTGGAGG AAATAATCCA CCACAACCGG ACAATGCATG TCAAGGAGTT TCCGTTTGTG 
TGCGAGACGT GCGGAGAGTC GTACTCACGC AAACAGCAGT TCCACGCCCA CGTGGAGTCG 
CACAATAAGA AGGAAATCAA AACCTTTCCC TGCGGCGAGT GCGGTCTTAA GTTTCCGCAA 
AAGAAACTGC AGCAACACTT CGAGGAGACG GGCCACAAGG CGGACGGCGC TATCTGCGAG 
GTGTGCGGCG AGGAGTTCCA GTCGAAGAAT GCCCTGTACC AGCACATTAT TCGTGTGCAC 
AAGCGGGACA ACTTCTTCGA GTGCCACATT TGCCATAACC GCTTCACGCT GAAAGCCAAC 
CTGGAGCGGC ACGTCCAGCT GCACACCGAG ATCAAACGGC CCTACGTGTG CGATCTGTGC 
GGCTCGTCCT ACTTCACATA TCCCGCGCTC AAGGAGCACT ACAGCAACGC CCACGTGGAC 
GTGTCCGAGT GCAAATGCAC GCTTTGCGGC AAGCGCTTCG GTTCGGCCAA GTCGCTGCAG 
CGACACCTTC CGTCGCACTC GGAGGAAAGG CCGCACTGCT GCAACTATTG CGATCAGACC 
TTCAAGTGGA AAACGCATCT GGTGCGCCAC AAGCAGACAA TGCACGGAAA CGAGCCGCCT 
CCGCCTAAGA AGGGAAAGCA GCGCTTTCCC AAAACAAGCG AAGAAGATAT GGGTAGCTTA 
CCGGACATGC CGGGTCCACC GCCTGTGAAG GCCAGCAAGA AGGCAGCAAC CAGCAAGGCG 
AAAGCGCAAG CAGCGGCCGC CGCAGCCGCA GCAGCATCAT CCCAGCAACA ACAACAGAAG 
GGTTCTGCAG CAACACCGAC GCCGCCACCG CCGGTCTCCA CCACACCGGG ATGCCTGCAA 
CAGGATCCGT TCAATGCGGC CATGGTTAGC AGCAACAGCT CGCAGTCCTC CACGGCGTCC 
ACGTCGCAAC ACTCGGTGTC GACGAGTGAA TCTCAGCAGA ATTCCATATA CAATCAGAGC 
TTTAATGCGG AGAAACAGCA GCCAGGACAG CAGCAGCCCC AGAATACCTT GCATCAGCAA 
CATCCAACGC CGCCACCTCC GCAGCAGCAG CAACAACAAC AGCAGCAGCA GCAGGCGCTG 
CCACAGTCAC GAGTTGGGCC CGGAGAACTG CATCTGCAGC GGATGAATAG TTTCGGCCAG 
GACGGACAGT TTCACTTTGA GTCCAATCCG GCAGCTGGTG CGTATCAACA GCATCAACTG 
ATCAGCCAGT ATCAGCAACA ACAGCAGCAG CAGCAGCAAC AACAGCAGCA GCAGCCCCAG 
CAGCAGCAAC AACATCATCT CGCCCAACAG CAGCAGCAGC ACATGAGGAG TCACACGCCC 
CAGAGTCCAC ATCCTCCGCA TCCTTCGCAG CTGCAACAAC AGCAGCCCCA ACAGCACTTT 
AGCTCTCCGC ACCACATGCC GCCAATGCAC CATCAACACC AGCTGATGAT GCAACAGCAA 
CATCGCCACA ACCAGCGCTC GCCGCTTCAC CATCATCCTG CGGCGCAGCA GCTTCAGCAG 
CAGCATCAGC AACCATCGCA TTCCCCACAG CATCAGCATG CGAGACTGCA GTCGCCACAA 
CCTGCTCCAG TACAACAACA ACAGCAGCAG CAGCAGCAAC AACAGCAGCA GCAGCAGCAG 
TTCCTGCAGC AACAGGCTGC CGACACGTGG GGCAACATGA ACTTTGGCAA TCCACCGAAC 
AATCAGCAGG TTGAGCTCAA GGATAACAAA TTCTATATTG TGGAATCGGC CGAGTTCCTA 
GGTCTTCCAA TGAATGTGCC GCCATCTCCG CAGCAGCAGC AACAGCAAGC AGTAAGCAAA 
CCCCAACAGC AGCAGCAACA ACAACACCCA CAACCGCAAC AGCCAGATCC AACCCTTGCT 
GGTGATTTGA TGAGCTTCCA GCATATGTGG CCGGCGCCTG GTTTTAGCCA GACTTCACAG 
CAGCAACAGC CACAGCAGCA ACAACAGCAG CAAGCGGCGC AGCAGCAACA GCAGGCGCAA 
AGTCAGGCAA ACTCAAGTGA TCCCCACAAC TACAACAACA TCGGCAGCAT ACTAACGAAT 
CTCATTGACA CAAATCCCGC GCCAATGGAG TACAACTTCG ATCTGATGCA GCAGCAACAA 
ACGCCGTCAG CCCAACAGCA GCAGCAGCAA CAACAACAAC AGCAGGCGGC GCAGCAGCAA 
CATCACAGTG CCGGTGCATA TGGGAGCGGT TTGATGCGCA GCGGAGGCGG CGTTTACGGA 
GGCGCCTACG AGCAGCACTT GAGTGACCAG CTGGTCAGCG AGCAGCAGAA ACAGAACAGC 
TTTCAACCCT ACCACCCACA TGGCCTGCAG GCGCAGCAAC AGCAGCCACT GCATCCGCAT 
CCGCATTTGT TACCCCCGCC AGCGCCGCAT AATAATGTCT ACGATCGCAC GGGAGTTGGA 
GTCGGTGTCG GAGTGGGCGT AGGCGTGGGA GTGGGTGTGG GCTATCCAAT GCTGGGAGAC 
GATACCAAGA GCGTCCTGCC GCCCATGATG GGCGGCATGA TGCAGCAAAT GCCACCGCAC 
GGGCAGCAGC CGGATCTAAT CTACTACCCA GTGAAGAACG ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726015.2
CDS	250..5154
Translation	MDNKTTQLDFKLLVDQSLNLLDSLNTALRCDAIQFLLQTLKCKYPESCDQVVRNLFSHIAAANDNGESGGRAQQHELARTKQLQLLLTAKKEKKELGIGCEAEIKRENDEEEAGSTDLNQNFEKIPCKEEFLCLEEEEEDFLDDADTQNSSSLQFHTGNNVDALALGPGDPLELIQSGVSLLVKRKYAATFDDELIGDGDGDEANSNSSDGKMVKRKRTNNMHLTVSSVRRKEEHGQLGDGYVFHEDSQYTMRYHHSTGEFSERAFKAQFRALLRLALSQHHKPFLRQFYENFVVNGRLLGTTPSMRVLKELREQRLEEWRRQRERRQLVILMEQSETQRDEEFQQQQELVENQEEVQQEQEQQQQQLEDIQQQHLEEIQRQQQLPAISFGDSELESETESQLSSAAQEIMKFEEFIDEGVGAGRLIDGLEMEPEIDDMLAGAGSMNSASGHSSNSSSSGSGSAGNGNGISGVILENNSHHTHHLSSSSSANLVINGLTSSNSISNNNNNSNNVSLHRPQLTPQAQNQLAASMKPSDHIPISMPMENMDLKAGQAAHGTANAIMQGGSSSLASQLHQQQKPYNSSNNPLSMMGGMMQQQQQQHVAMPHHQRQMMMMPEDFPQHGASMMNSRQMPALAALSNLGDTPAMSGQLNSSLELDDNDLSADEDDDDLDHDLDELDAAKQQLIDGGSSSSTSLQAPPSQHGSGSGGSGGQTPGSKKDKPSYNCLLCPKSYRKRKSLLDHYKMHPGYCHDCGQRNGNTLEEIIHHNRTMHVKEFPFVCETCGESYSRKQQFHAHVESHNKKEIKTFPCGECGLKFPQKKLQQHFEETGHKADGAICEVCGEEFQSKNALYQHIIRVHKRDNFFECHICHNRFTLKANLERHVQLHTEIKRPYVCDLCGSSYFTYPALKEHYSNAHVDVSECKCTLCGKRFGSAKSLQRHLPSHSEERPHCCNYCDQTFKWKTHLVRHKQTMHGNEPPPPKKGKQRFPKTSEEDMGSLPDMPGPPPVKASKKAATSKAKAQAAAAAAAAASSQQQQQKGSAATPTPPPPVSTTPGCLQQDPFNAAMVSSNSSQSSTASTSQHSVSTSESQQNSIYNQSFNAEKQQPGQQQPQNTLHQQHPTPPPPQQQQQQQQQQQALPQSRVGPGELHLQRMNSFGQDGQFHFESNPAAGAYQQHQLISQYQQQQQQQQQQQQQQPQQQQQHHLAQQQQQHMRSHTPQSPHPPHPSQLQQQQPQQHFSSPHHMPPMHHQHQLMMQQQHRHNQRSPLHHHPAAQQLQQQHQQPSHSPQHQHARLQSPQPAPVQQQQQQQQQQQQQQQQFLQQQAADTWGNMNFGNPPNNQQVELKDNKFYIVESAEFLGLPMNVPPSPQQQQQQAVSKPQQQQQQQHPQPQQPDPTLAGDLMSFQHMWPAPGFSQTSQQQQPQQQQQQQAAQQQQQAQSQANSSDPHNYNNIGSILTNLIDTNPAPMEYNFDLMQQQQTPSAQQQQQQQQQQQAAQQQHHSAGAYGSGLMRSGGGVYGGAYEQHLSDQLVSEQQKQNSFQPYHPHGLQAQQQQPLHPHPHLLPPPAPHNNVYDRTGVGVGVGVGVGVGVGVGYPMLGDDTKSVLPPMMGGMMQQMPPHGQQPDLIYYPVKND

Target ORF information:

RefSeq Version	NM_166416.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant E (CG10543), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166416.2

ORF Insert Sequence:

ATGGATAACA AAACTACACA GTTGGATTTT AAATTACTGG TGGATCAATC ACTAAATCTA 
CTGGACAGCC TCAACACGGC ATTGCGGTGC GATGCTATTC AATTCTTGCT GCAGACGCTT 
AAGTGCAAAT ACCCGGAGTC CTGCGACCAA GTCGTCAGGA ATCTGTTCAG TCACATAGCT 
GCGGCCAATG ACAATGGCGA GTCTGGAGGC AGGGCGCAGC AGCACGAGCT AGCGAGGACG 
AAGCAGCTCC AGCTGCTGCT GACCGCCAAA AAGGAGAAGA AAGAGCTGGG AATCGGTTGC 
GAGGCGGAGA TCAAGCGAGA GAACGATGAG GAGGAGGCGG GCTCGACGGA TCTGAACCAA 
AATTTCGAGA AGATACCCTG CAAAGAGGAG TTCCTGTGCC TCGAGGAGGA AGAGGAGGAC 
TTTCTCGACG ATGCGGACAC GCAGAACTCG TCTTCCCTGC AGTTCCACAC CGGTAACAAT 
GTGGACGCCC TGGCCCTGGG CCCTGGCGAT CCGCTGGAGC TGATCCAGTC CGGAGTTTCG 
CTGCTGGTCA AGCGGAAGTA TGCTGCCACC TTCGATGACG AACTGATCGG GGATGGGGAT 
GGTGATGAGG CCAACAGTAA CAGCAGTGAC GGCAAGATGG TCAAGCGAAA GCGCACCAAC 
AACATGCACC TGACCGTGTC GAGTGTGCGA CGGAAAGAGG AGCACGGCCA GCTGGGCGAT 
GGCTATGTCT TCCACGAGGA CAGCCAGTAT ACGATGCGAT ATCACCACAG CACCGGCGAG 
TTCAGTGAGC GGGCCTTTAA GGCCCAGTTC CGGGCTCTGC TCCGCCTGGC GCTCAGTCAG 
CACCACAAGC CCTTTCTCCG CCAGTTCTAC GAGAACTTTG TTGTGAATGG ACGCTTGCTG 
GGGACCACGC CCTCGATGCG TGTCCTGAAG GAGCTGCGCG AACAGCGGCT GGAGGAGTGG 
CGACGTCAGC GTGAACGCCG CCAACTGGTC ATCCTGATGG AGCAGAGCGA GACTCAGCGG 
GACGAAGAGT TTCAGCAGCA GCAGGAGCTG GTGGAGAACC AAGAGGAGGT CCAGCAGGAG 
CAAGAACAGC AACAGCAGCA GCTGGAGGAT ATTCAGCAGC AGCATCTGGA GGAGATTCAG 
CGGCAGCAGC AATTGCCGGC CATCTCGTTT GGCGACTCCG AATTGGAGTC AGAAACGGAA 
TCGCAGCTGT CGTCGGCTGC CCAAGAGATT ATGAAGTTTG AGGAGTTCAT TGACGAGGGT 
GTAGGCGCTG GCCGTCTGAT CGATGGTCTG GAGATGGAAC CCGAGATCGA TGACATGCTA 
GCTGGCGCCG GCAGTATGAA CAGCGCCAGT GGACACAGCA GCAACAGTAG CAGCAGCGGA 
AGTGGGAGCG CCGGTAATGG GAATGGCATC AGCGGGGTGA TCCTGGAGAA CAATAGTCAT 
CATACGCATC ACCTAAGTAG CAGCAGCAGC GCAAATCTTG TGATCAACGG CCTTACGTCA 
AGCAATAGCA TCAGCAACAA TAATAATAAC AGCAACAATG TCAGTTTACA CCGCCCTCAA 
TTGACGCCAC AGGCGCAGAA TCAGCTGGCA GCGTCGATGA AACCATCAGA CCATATTCCC 
ATATCGATGC CCATGGAGAA CATGGATTTG AAAGCCGGGC AGGCGGCTCA TGGGACTGCC 
AACGCCATCA TGCAGGGCGG TAGTTCGAGC CTCGCGAGCC AGCTACATCA GCAGCAAAAA 
CCCTACAACT CAAGCAATAA TCCGCTGTCT ATGATGGGTG GAATGATGCA GCAGCAGCAG 
CAGCAGCATG TTGCTATGCC GCACCACCAG CGCCAGATGA TGATGATGCC AGAGGATTTC 
CCGCAACACG GAGCCTCAAT GATGAACAGT CGTCAGATGC CCGCTCTGGC CGCCTTGTCG 
AATCTGGGCG ACACACCGGC TATGAGTGGC CAGCTTAACT CCTCCCTAGA GCTGGACGAC 
AATGACCTGT CGGCGGATGA GGACGACGAC GATCTGGACC ATGACCTGGA CGAATTAGAC 
GCGGCCAAGC AACAACTAAT AGATGGCGGC AGCAGTAGCA GCACATCGCT TCAGGCGCCA 
CCATCGCAGC ACGGCAGCGG TAGTGGAGGC AGCGGCGGCC AGACGCCCGG TAGCAAAAAG 
GATAAGCCCA GCTACAACTG CCTGCTCTGC CCCAAGTCGT ATCGCAAGCG CAAGTCCTTG 
CTAGACCACT ACAAAATGCA TCCGGGCTAC TGCCATGACT GCGGTCAGCG CAATGGGAAC 
ACGCTGGAGG AAATAATCCA CCACAACCGG ACAATGCATG TCAAGGAGTT TCCGTTTGTG 
TGCGAGACGT GCGGAGAGTC GTACTCACGC AAACAGCAGT TCCACGCCCA CGTGGAGTCG 
CACAATAAGA AGGAAATCAA AACCTTTCCC TGCGGCGAGT GCGGTCTTAA GTTTCCGCAA 
AAGAAACTGC AGCAACACTT CGAGGAGACG GGCCACAAGG CGGACGGCGC TATCTGCGAG 
GTGTGCGGCG AGGAGTTCCA GTCGAAGAAT GCCCTGTACC AGCACATTAT TCGTGTGCAC 
AAGCGGGACA ACTTCTTCGA GTGCCACATT TGCCATAACC GCTTCACGCT GAAAGCCAAC 
CTGGAGCGGC ACGTCCAGCT GCACACCGAG ATCAAACGGC CCTACGTGTG CGATCTGTGC 
GGCTCGTCCT ACTTCACATA TCCCGCGCTC AAGGAGCACT ACAGCAACGC CCACGTGGAC 
GTGTCCGAGT GCAAATGCAC GCTTTGCGGC AAGCGCTTCG GTTCGGCCAA GTCGCTGCAG 
CGACACCTTC CGTCGCACTC GGAGGAAAGG CCGCACTGCT GCAACTATTG CGATCAGACC 
TTCAAGTGGA AAACGCATCT GGTGCGCCAC AAGCAGACAA TGCACGGAAA CGAGCCGCCT 
CCGCCTAAGA AGGGAAAGCA GCGCTTTCCC AAAACAAGCG AAGAAGATAT GGGTAGCTTA 
CCGGACATGC CGGGTCCACC GCCTGTGAAG GCCAGCAAGA AGGCAGCAAC CAGCAAGGCG 
AAAGCGCAAG CAGCGGCCGC CGCAGCCGCA GCAGCATCAT CCCAGCAACA ACAACAGAAG 
GGTTCTGCAG CAACACCGAC GCCGCCACCG CCGGTCTCCA CCACACCGGG ATGCCTGCAA 
CAGGATCCGT TCAATGCGGC CATGGTTAGC AGCAACAGCT CGCAGTCCTC CACGGCGTCC 
ACGTCGCAAC ACTCGGTGTC GACGAGTGAA TCTCAGCAGA ATTCCATATA CAATCAGAGC 
TTTAATGCGG AGAAACAGCA GCCAGGACAG CAGCAGCCCC AGAATACCTT GCATCAGCAA 
CATCCAACGC CGCCACCTCC GCAGCAGCAG CAACAACAAC AGCAGCAGCA GCAGGCGCTG 
CCACAGTCAC GAGTTGGGCC CGGAGAACTG CATCTGCAGC GGATGAATAG TTTCGGCCAG 
GACGGACAGT TTCACTTTGA GTCCAATCCG GCAGCTGGTG CGTATCAACA GCATCAACTG 
ATCAGCCAGT ATCAGCAACA ACAGCAGCAG CAGCAGCAAC AACAGCAGCA GCAGCCCCAG 
CAGCAGCAAC AACATCATCT CGCCCAACAG CAGCAGCAGC ACATGAGGAG TCACACGCCC 
CAGAGTCCAC ATCCTCCGCA TCCTTCGCAG CTGCAACAAC AGCAGCCCCA ACAGCACTTT 
AGCTCTCCGC ACCACATGCC GCCAATGCAC CATCAACACC AGCTGATGAT GCAACAGCAA 
CATCGCCACA ACCAGCGCTC GCCGCTTCAC CATCATCCTG CGGCGCAGCA GCTTCAGCAG 
CAGCATCAGC AACCATCGCA TTCCCCACAG CATCAGCATG CGAGACTGCA GTCGCCACAA 
CCTGCTCCAG TACAACAACA ACAGCAGCAG CAGCAGCAAC AACAGCAGCA GCAGCAGCAG 
TTCCTGCAGC AACAGGCTGC CGACACGTGG GGCAACATGA ACTTTGGCAA TCCACCGAAC 
AATCAGCAGG TTGAGCTCAA GGATAACAAA TTCTATATTG TGGAATCGGC CGAGTTCCTA 
GGTCTTCCAA TGAATGTGCC GCCATCTCCG CAGCAGCAGC AACAGCAAGC AGTAAGCAAA 
CCCCAACAGC AGCAGCAACA ACAACACCCA CAACCGCAAC AGCCAGATCC AACCCTTGCT 
GGTGATTTGA TGAGCTTCCA GCATATGTGG CCGGCGCCTG GTTTTAGCCA GACTTCACAG 
CAGCAACAGC CACAGCAGCA ACAACAGCAG CAAGCGGCGC AGCAGCAACA GCAGGCGCAA 
AGTCAGGCAA ACTCAAGTGA TCCCCACAAC TACAACAACA TCGGCAGCAT ACTAACGAAT 
CTCATTGACA CAAATCCCGC GCCAATGGAG TACAACTTCG ATCTGATGCA GCAGCAACAA 
ACGCCGTCAG CCCAACAGCA GCAGCAGCAA CAACAACAAC AGCAGGCGGC GCAGCAGCAA 
CATCACAGTG CCGGTGCATA TGGGAGCGGT TTGATGCGCA GCGGAGGCGG CGTTTACGGA 
GGCGCCTACG AGCAGCACTT GAGTGACCAG CTGGTCAGCG AGCAGCAGAA ACAGAACAGC 
TTTCAACCCT ACCACCCACA TGGCCTGCAG GCGCAGCAAC AGCAGCCACT GCATCCGCAT 
CCGCATTTGT TACCCCCGCC AGCGCCGCAT AATAATGTCT ACGATCGCAC GGGAGTTGGA 
GTCGGTGTCG GAGTGGGCGT AGGCGTGGGA GTGGGTGTGG GCTATCCAAT GCTGGGAGAC 
GATACCAAGA GCGTCCTGCC GCCCATGATG GGCGGCATGA TGCAGCAAAT GCCACCGCAC 
GGGCAGCAGC CGGATCTAAT CTACTACCCA GTGAAGAACG ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG10543 ( NM_176241.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788421.2 Drosophila melanogaster uncharacterized protein, transcript variant D (CG10543), mRNA.

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CloneID	OFa10731
Clone ID Related Accession (Same CDS sequence)	NM_176241.3
Accession Version	NM_176241.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_176241.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAATGG GCGGCTTGAA GCGGAAGTTG GTGGATCAAT CACTAAATCT ACTGGACAGC 
CTCAACACGG CATTGCGGTG CGATGCTATT CAATTCTTGC TGCAGACGCT TAAGTGCAAA 
TACCCGGAGT CCTGCGACCA AGTCGTCAGG AATCTGTTCA GTCACATAGC TGCGGCCAAT 
GACAATGGCG AGTCTGGAGG CAGGGCGCAG CAGCACGAGC TAGCGAGGAC GAAGCAGCTC 
CAGCTGCTGC TGACCGCCAA AAAGGAGAAG AAAGAGCTGG GAATCGGTTG CGAGGCGGAG 
ATCAAGCGAG AGAACGATGA GGAGGAGGCG GGCTCGACGG ATCTGAACCA AAATTTCGAG 
AAGATACCCT GCAAAGAGGA GTTCCTGTGC CTCGAGGAGG AAGAGGAGGA CTTTCTCGAC 
GATGCGGACA CGCAGAACTC GTCTTCCCTG CAGTTCCACA CCGGTAACAA TGTGGACGCC 
CTGGCCCTGG GCCCTGGCGA TCCGCTGGAG CTGATCCAGT CCGGAGTTTC GCTGCTGGTC 
AAGCGGAAGT ATGCTGCCAC CTTCGATGAC GAACTGATCG GGGATGGGGA TGGTGATGAG 
GCCAACAGTA ACAGCAGTGA CGGCAAGATG GTCAAGCGAA AGCGCACCAA CAACATGCAC 
CTGACCGTGT CGAGTGTGCG ACGGAAAGAG GAGCACGGCC AGCTGGGCGA TGGCTATGTC 
TTCCACGAGG ACAGCCAGTA TACGATGCGA TATCACCACA GCACCGGCGA GTTCAGTGAG 
CGGGCCTTTA AGGCCCAGTT CCGGGCTCTG CTCCGCCTGG CGCTCAGTCA GCACCACAAG 
CCCTTTCTCC GCCAGTTCTA CGAGAACTTT GTTGTGAATG GACGCTTGCT GGGGACCACG 
CCCTCGATGC GTGTCCTGAA GGAGCTGCGC GAACAGCGGC TGGAGGAGTG GCGACGTCAG 
CGTGAACGCC GCCAACTGGT CATCCTGATG GAGCAGAGCG AGACTCAGCG GGACGAAGAG 
TTTCAGCAGC AGCAGGAGCT GGTGGAGAAC CAAGAGGAGG TCCAGCAGGA GCAAGAACAG 
CAACAGCAGC AGCTGGAGGA TATTCAGCAG CAGCATCTGG AGGAGATTCA GCGGCAGCAG 
CAATTGCCGG CCATCTCGTT TGGCGACTCC GAATTGGAGT CAGAAACGGA ATCGCAGCTG 
TCGTCGGCTG CCCAAGAGAT TATGAAGTTT GAGGAGTTCA TTGACGAGGG TGTAGGCGCT 
GGCCGTCTGA TCGATGGTCT GGAGATGGAA CCCGAGATCG ATGACATGCT AGCTGGCGCC 
GGCAGTATGA ACAGCGCCAG TGGACACAGC AGCAACAGTA GCAGCAGCGG AAGTGGGAGC 
GCCGGTAATG GGAATGGCAT CAGCGGGGTG ATCCTGGAGA ACAATAGTCA TCATACGCAT 
CACCTAAGTA GCAGCAGCAG CGCAAATCTT GTGATCAACG GCCTTACGTC AAGCAATAGC 
ATCAGCAACA ATAATAATAA CAGCAACAAT GTCAGTTTAC ACCGCCCTCA ATTGACGCCA 
CAGGCGCAGA ATCAGCTGGC AGCGTCGATG AAACCATCAG ACCATATTCC CATATCGATG 
CCCATGGAGA ACATGGATTT GAAAGCCGGG CAGGCGGCTC ATGGGACTGC CAACGCCATC 
ATGCAGGGCG GTAGTTCGAG CCTCGCGAGC CAGCTACATC AGCAGCAAAA ACCCTACAAC 
TCAAGCAATA ATCCGCTGTC TATGATGGGT GGAATGATGC AGCAGCAGCA GCAGCAGCAT 
GTTGCTATGC CGCACCACCA GCGCCAGATG ATGATGATGC CAGAGGATTT CCCGCAACAC 
GGAGCCTCAA TGATGAACAG TCGTCAGATG CCCGCTCTGG CCGCCTTGTC GAATCTGGGC 
GACACACCGG CTATGAGTGG CCAGCTTAAC TCCTCCCTAG AGCTGGACGA CAATGACCTG 
TCGGCGGATG AGGACGACGA CGATCTGGAC CATGACCTGG ACGAATTAGA CGCGGCCAAG 
CAACAACTAA TAGATGGCGG CAGCAGTAGC AGCACATCGC TTCAGGCGCC ACCATCGCAG 
CACGGCAGCG GTAGTGGAGG CAGCGGCGGC CAGACGCCCG GTAGCAAAAA GGATAAGCCC 
AGCTACAACT GCCTGCTCTG CCCCAAGTCG TATCGCAAGC GCAAGTCCTT GCTAGACCAC 
TACAAAATGC ATCCGGGCTA CTGCCATGAC TGCGGTCAGC GCAATGGGAA CACGCTGGAG 
GAAATAATCC ACCACAACCG GACAATGCAT GTCAAGGAGT TTCCGTTTGT GTGCGAGACG 
TGCGGAGAGT CGTACTCACG CAAACAGCAG TTCCACGCCC ACGTGGAGTC GCACAATAAG 
AAGGAAATCA AAACCTTTCC CTGCGGCGAG TGCGGTCTTA AGTTTCCGCA AAAGAAACTG 
CAGCAACACT TCGAGGAGAC GGGCCACAAG GCGGACGGCG CTATCTGCGA GGTGTGCGGC 
GAGGAGTTCC AGTCGAAGAA TGCCCTGTAC CAGCACATTA TTCGTGTGCA CAAGCGGGAC 
AACTTCTTCG AGTGCCACAT TTGCCATAAC CGCTTCACGC TGAAAGCCAA CCTGGAGCGG 
CACGTCCAGC TGCACACCGA GATCAAACGG CCCTACGTGT GCGATCTGTG CGGCTCGTCC 
TACTTCACAT ATCCCGCGCT CAAGGAGCAC TACAGCAACG CCCACGTGGA CGTGTCCGAG 
TGCAAATGCA CGCTTTGCGG CAAGCGCTTC GGTTCGGCCA AGTCGCTGCA GCGACACCTT 
CCGTCGCACT CGGAGGAAAG GCCGCACTGC TGCAACTATT GCGATCAGAC CTTCAAGTGG 
AAAACGCATC TGGTGCGCCA CAAGCAGACA ATGCACGGAA ACGAGCCGCC TCCGCCTAAG 
AAGGGAAAGC AGCGCTTTCC CAAAACAAGC GAAGAAGATA TGGGTAGCTT ACCGGACATG 
CCGGGTCCAC CGCCTGTGAA GGCCAGCAAG AAGGCAGCAA CCAGCAAGGC GAAAGCGCAA 
GCAGCGGCCG CCGCAGCCGC AGCAGCATCA TCCCAGCAAC AACAACAGAA GGGTTCTGCA 
GCAACACCGA CGCCGCCACC GCCGGTCTCC ACCACACCGG GATGCCTGCA ACAGGATCCG 
TTCAATGCGG CCATGGTTAG CAGCAACAGC TCGCAGTCCT CCACGGCGTC CACGTCGCAA 
CACTCGGTGT CGACGAGTGA ATCTCAGCAG AATTCCATAT ACAATCAGAG CTTTAATGCG 
GAGAAACAGC AGCCAGGACA GCAGCAGCCC CAGAATACCT TGCATCAGCA ACATCCAACG 
CCGCCACCTC CGCAGCAGCA GCAACAACAA CAGCAGCAGC AGCAGGCGCT GCCACAGTCA 
CGAGTTGGGC CCGGAGAACT GCATCTGCAG CGGATGAATA GTTTCGGCCA GGACGGACAG 
TTTCACTTTG AGTCCAATCC GGCAGCTGGT GCGTATCAAC AGCATCAACT GATCAGCCAG 
TATCAGCAAC AACAGCAGCA GCAGCAGCAA CAACAGCAGC AGCAGCCCCA GCAGCAGCAA 
CAACATCATC TCGCCCAACA GCAGCAGCAG CACATGAGGA GTCACACGCC CCAGAGTCCA 
CATCCTCCGC ATCCTTCGCA GCTGCAACAA CAGCAGCCCC AACAGCACTT TAGCTCTCCG 
CACCACATGC CGCCAATGCA CCATCAACAC CAGCTGATGA TGCAACAGCA ACATCGCCAC 
AACCAGCGCT CGCCGCTTCA CCATCATCCT GCGGCGCAGC AGCTTCAGCA GCAGCATCAG 
CAACCATCGC ATTCCCCACA GCATCAGCAT GCGAGACTGC AGTCGCCACA ACCTGCTCCA 
GTACAACAAC AACAGCAGCA GCAGCAGCAA CAACAGCAGC AGCAGCAGCA GTTCCTGCAG 
CAACAGGCTG CCGACACGTG GGGCAACATG AACTTTGGCA ATCCACCGAA CAATCAGCAG 
GTTGAGCTCA AGGATAACAA ATTCTATATT GTGGAATCGG CCGAGTTCCT AGGTCTTCCA 
ATGAATGTGC CGCCATCTCC GCAGCAGCAG CAACAGCAAG CAGTAAGCAA ACCCCAACAG 
CAGCAGCAAC AACAACACCC ACAACCGCAA CAGCCAGATC CAACCCTTGC TGGTGATTTG 
ATGAGCTTCC AGCATATGTG GCCGGCGCCT GGTTTTAGCC AGACTTCACA GCAGCAACAG 
CCACAGCAGC AACAACAGCA GCAAGCGGCG CAGCAGCAAC AGCAGGCGCA AAGTCAGGCA 
AACTCAAGTG ATCCCCACAA CTACAACAAC ATCGGCAGCA TACTAACGAA TCTCATTGAC 
ACAAATCCCG CGCCAATGGA GTACAACTTC GATCTGATGC AGCAGCAACA AACGCCGTCA 
GCCCAACAGC AGCAGCAGCA ACAACAACAA CAGCAGGCGG CGCAGCAGCA ACATCACAGT 
GCCGGTGCAT ATGGGAGCGG TTTGATGCGC AGCGGAGGCG GCGTTTACGG AGGCGCCTAC 
GAGCAGCACT TGAGTGACCA GCTGGTCAGC GAGCAGCAGA AACAGAACAG CTTTCAACCC 
TACCACCCAC ATGGCCTGCA GGCGCAGCAA CAGCAGCCAC TGCATCCGCA TCCGCATTTG 
TTACCCCCGC CAGCGCCGCA TAATAATGTC TACGATCGCA CGGGAGTTGG AGTCGGTGTC 
GGAGTGGGCG TAGGCGTGGG AGTGGGTGTG GGCTATCCAA TGCTGGGAGA CGATACCAAG 
AGCGTCCTGC CGCCCATGAT GGGCGGCATG ATGCAGCAAA TGCCACCGCA CGGGCAGCAG 
CCGGATCTAA TCTACTACCC AGTGAAGAAC GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788421.2
CDS	327..5222
Translation	MEMGGLKRKLVDQSLNLLDSLNTALRCDAIQFLLQTLKCKYPESCDQVVRNLFSHIAAANDNGESGGRAQQHELARTKQLQLLLTAKKEKKELGIGCEAEIKRENDEEEAGSTDLNQNFEKIPCKEEFLCLEEEEEDFLDDADTQNSSSLQFHTGNNVDALALGPGDPLELIQSGVSLLVKRKYAATFDDELIGDGDGDEANSNSSDGKMVKRKRTNNMHLTVSSVRRKEEHGQLGDGYVFHEDSQYTMRYHHSTGEFSERAFKAQFRALLRLALSQHHKPFLRQFYENFVVNGRLLGTTPSMRVLKELREQRLEEWRRQRERRQLVILMEQSETQRDEEFQQQQELVENQEEVQQEQEQQQQQLEDIQQQHLEEIQRQQQLPAISFGDSELESETESQLSSAAQEIMKFEEFIDEGVGAGRLIDGLEMEPEIDDMLAGAGSMNSASGHSSNSSSSGSGSAGNGNGISGVILENNSHHTHHLSSSSSANLVINGLTSSNSISNNNNNSNNVSLHRPQLTPQAQNQLAASMKPSDHIPISMPMENMDLKAGQAAHGTANAIMQGGSSSLASQLHQQQKPYNSSNNPLSMMGGMMQQQQQQHVAMPHHQRQMMMMPEDFPQHGASMMNSRQMPALAALSNLGDTPAMSGQLNSSLELDDNDLSADEDDDDLDHDLDELDAAKQQLIDGGSSSSTSLQAPPSQHGSGSGGSGGQTPGSKKDKPSYNCLLCPKSYRKRKSLLDHYKMHPGYCHDCGQRNGNTLEEIIHHNRTMHVKEFPFVCETCGESYSRKQQFHAHVESHNKKEIKTFPCGECGLKFPQKKLQQHFEETGHKADGAICEVCGEEFQSKNALYQHIIRVHKRDNFFECHICHNRFTLKANLERHVQLHTEIKRPYVCDLCGSSYFTYPALKEHYSNAHVDVSECKCTLCGKRFGSAKSLQRHLPSHSEERPHCCNYCDQTFKWKTHLVRHKQTMHGNEPPPPKKGKQRFPKTSEEDMGSLPDMPGPPPVKASKKAATSKAKAQAAAAAAAAASSQQQQQKGSAATPTPPPPVSTTPGCLQQDPFNAAMVSSNSSQSSTASTSQHSVSTSESQQNSIYNQSFNAEKQQPGQQQPQNTLHQQHPTPPPPQQQQQQQQQQQALPQSRVGPGELHLQRMNSFGQDGQFHFESNPAAGAYQQHQLISQYQQQQQQQQQQQQQQPQQQQQHHLAQQQQQHMRSHTPQSPHPPHPSQLQQQQPQQHFSSPHHMPPMHHQHQLMMQQQHRHNQRSPLHHHPAAQQLQQQHQQPSHSPQHQHARLQSPQPAPVQQQQQQQQQQQQQQQQFLQQQAADTWGNMNFGNPPNNQQVELKDNKFYIVESAEFLGLPMNVPPSPQQQQQQAVSKPQQQQQQQHPQPQQPDPTLAGDLMSFQHMWPAPGFSQTSQQQQPQQQQQQQAAQQQQQAQSQANSSDPHNYNNIGSILTNLIDTNPAPMEYNFDLMQQQQTPSAQQQQQQQQQQQAAQQQHHSAGAYGSGLMRSGGGVYGGAYEQHLSDQLVSEQQKQNSFQPYHPHGLQAQQQQPLHPHPHLLPPPAPHNNVYDRTGVGVGVGVGVGVGVGVGYPMLGDDTKSVLPPMMGGMMQQMPPHGQQPDLIYYPVKND

Target ORF information:

RefSeq Version	NM_176241.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant D (CG10543), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176241.3

ORF Insert Sequence:

ATGGAAATGG GCGGCTTGAA GCGGAAGTTG GTGGATCAAT CACTAAATCT ACTGGACAGC 
CTCAACACGG CATTGCGGTG CGATGCTATT CAATTCTTGC TGCAGACGCT TAAGTGCAAA 
TACCCGGAGT CCTGCGACCA AGTCGTCAGG AATCTGTTCA GTCACATAGC TGCGGCCAAT 
GACAATGGCG AGTCTGGAGG CAGGGCGCAG CAGCACGAGC TAGCGAGGAC GAAGCAGCTC 
CAGCTGCTGC TGACCGCCAA AAAGGAGAAG AAAGAGCTGG GAATCGGTTG CGAGGCGGAG 
ATCAAGCGAG AGAACGATGA GGAGGAGGCG GGCTCGACGG ATCTGAACCA AAATTTCGAG 
AAGATACCCT GCAAAGAGGA GTTCCTGTGC CTCGAGGAGG AAGAGGAGGA CTTTCTCGAC 
GATGCGGACA CGCAGAACTC GTCTTCCCTG CAGTTCCACA CCGGTAACAA TGTGGACGCC 
CTGGCCCTGG GCCCTGGCGA TCCGCTGGAG CTGATCCAGT CCGGAGTTTC GCTGCTGGTC 
AAGCGGAAGT ATGCTGCCAC CTTCGATGAC GAACTGATCG GGGATGGGGA TGGTGATGAG 
GCCAACAGTA ACAGCAGTGA CGGCAAGATG GTCAAGCGAA AGCGCACCAA CAACATGCAC 
CTGACCGTGT CGAGTGTGCG ACGGAAAGAG GAGCACGGCC AGCTGGGCGA TGGCTATGTC 
TTCCACGAGG ACAGCCAGTA TACGATGCGA TATCACCACA GCACCGGCGA GTTCAGTGAG 
CGGGCCTTTA AGGCCCAGTT CCGGGCTCTG CTCCGCCTGG CGCTCAGTCA GCACCACAAG 
CCCTTTCTCC GCCAGTTCTA CGAGAACTTT GTTGTGAATG GACGCTTGCT GGGGACCACG 
CCCTCGATGC GTGTCCTGAA GGAGCTGCGC GAACAGCGGC TGGAGGAGTG GCGACGTCAG 
CGTGAACGCC GCCAACTGGT CATCCTGATG GAGCAGAGCG AGACTCAGCG GGACGAAGAG 
TTTCAGCAGC AGCAGGAGCT GGTGGAGAAC CAAGAGGAGG TCCAGCAGGA GCAAGAACAG 
CAACAGCAGC AGCTGGAGGA TATTCAGCAG CAGCATCTGG AGGAGATTCA GCGGCAGCAG 
CAATTGCCGG CCATCTCGTT TGGCGACTCC GAATTGGAGT CAGAAACGGA ATCGCAGCTG 
TCGTCGGCTG CCCAAGAGAT TATGAAGTTT GAGGAGTTCA TTGACGAGGG TGTAGGCGCT 
GGCCGTCTGA TCGATGGTCT GGAGATGGAA CCCGAGATCG ATGACATGCT AGCTGGCGCC 
GGCAGTATGA ACAGCGCCAG TGGACACAGC AGCAACAGTA GCAGCAGCGG AAGTGGGAGC 
GCCGGTAATG GGAATGGCAT CAGCGGGGTG ATCCTGGAGA ACAATAGTCA TCATACGCAT 
CACCTAAGTA GCAGCAGCAG CGCAAATCTT GTGATCAACG GCCTTACGTC AAGCAATAGC 
ATCAGCAACA ATAATAATAA CAGCAACAAT GTCAGTTTAC ACCGCCCTCA ATTGACGCCA 
CAGGCGCAGA ATCAGCTGGC AGCGTCGATG AAACCATCAG ACCATATTCC CATATCGATG 
CCCATGGAGA ACATGGATTT GAAAGCCGGG CAGGCGGCTC ATGGGACTGC CAACGCCATC 
ATGCAGGGCG GTAGTTCGAG CCTCGCGAGC CAGCTACATC AGCAGCAAAA ACCCTACAAC 
TCAAGCAATA ATCCGCTGTC TATGATGGGT GGAATGATGC AGCAGCAGCA GCAGCAGCAT 
GTTGCTATGC CGCACCACCA GCGCCAGATG ATGATGATGC CAGAGGATTT CCCGCAACAC 
GGAGCCTCAA TGATGAACAG TCGTCAGATG CCCGCTCTGG CCGCCTTGTC GAATCTGGGC 
GACACACCGG CTATGAGTGG CCAGCTTAAC TCCTCCCTAG AGCTGGACGA CAATGACCTG 
TCGGCGGATG AGGACGACGA CGATCTGGAC CATGACCTGG ACGAATTAGA CGCGGCCAAG 
CAACAACTAA TAGATGGCGG CAGCAGTAGC AGCACATCGC TTCAGGCGCC ACCATCGCAG 
CACGGCAGCG GTAGTGGAGG CAGCGGCGGC CAGACGCCCG GTAGCAAAAA GGATAAGCCC 
AGCTACAACT GCCTGCTCTG CCCCAAGTCG TATCGCAAGC GCAAGTCCTT GCTAGACCAC 
TACAAAATGC ATCCGGGCTA CTGCCATGAC TGCGGTCAGC GCAATGGGAA CACGCTGGAG 
GAAATAATCC ACCACAACCG GACAATGCAT GTCAAGGAGT TTCCGTTTGT GTGCGAGACG 
TGCGGAGAGT CGTACTCACG CAAACAGCAG TTCCACGCCC ACGTGGAGTC GCACAATAAG 
AAGGAAATCA AAACCTTTCC CTGCGGCGAG TGCGGTCTTA AGTTTCCGCA AAAGAAACTG 
CAGCAACACT TCGAGGAGAC GGGCCACAAG GCGGACGGCG CTATCTGCGA GGTGTGCGGC 
GAGGAGTTCC AGTCGAAGAA TGCCCTGTAC CAGCACATTA TTCGTGTGCA CAAGCGGGAC 
AACTTCTTCG AGTGCCACAT TTGCCATAAC CGCTTCACGC TGAAAGCCAA CCTGGAGCGG 
CACGTCCAGC TGCACACCGA GATCAAACGG CCCTACGTGT GCGATCTGTG CGGCTCGTCC 
TACTTCACAT ATCCCGCGCT CAAGGAGCAC TACAGCAACG CCCACGTGGA CGTGTCCGAG 
TGCAAATGCA CGCTTTGCGG CAAGCGCTTC GGTTCGGCCA AGTCGCTGCA GCGACACCTT 
CCGTCGCACT CGGAGGAAAG GCCGCACTGC TGCAACTATT GCGATCAGAC CTTCAAGTGG 
AAAACGCATC TGGTGCGCCA CAAGCAGACA ATGCACGGAA ACGAGCCGCC TCCGCCTAAG 
AAGGGAAAGC AGCGCTTTCC CAAAACAAGC GAAGAAGATA TGGGTAGCTT ACCGGACATG 
CCGGGTCCAC CGCCTGTGAA GGCCAGCAAG AAGGCAGCAA CCAGCAAGGC GAAAGCGCAA 
GCAGCGGCCG CCGCAGCCGC AGCAGCATCA TCCCAGCAAC AACAACAGAA GGGTTCTGCA 
GCAACACCGA CGCCGCCACC GCCGGTCTCC ACCACACCGG GATGCCTGCA ACAGGATCCG 
TTCAATGCGG CCATGGTTAG CAGCAACAGC TCGCAGTCCT CCACGGCGTC CACGTCGCAA 
CACTCGGTGT CGACGAGTGA ATCTCAGCAG AATTCCATAT ACAATCAGAG CTTTAATGCG 
GAGAAACAGC AGCCAGGACA GCAGCAGCCC CAGAATACCT TGCATCAGCA ACATCCAACG 
CCGCCACCTC CGCAGCAGCA GCAACAACAA CAGCAGCAGC AGCAGGCGCT GCCACAGTCA 
CGAGTTGGGC CCGGAGAACT GCATCTGCAG CGGATGAATA GTTTCGGCCA GGACGGACAG 
TTTCACTTTG AGTCCAATCC GGCAGCTGGT GCGTATCAAC AGCATCAACT GATCAGCCAG 
TATCAGCAAC AACAGCAGCA GCAGCAGCAA CAACAGCAGC AGCAGCCCCA GCAGCAGCAA 
CAACATCATC TCGCCCAACA GCAGCAGCAG CACATGAGGA GTCACACGCC CCAGAGTCCA 
CATCCTCCGC ATCCTTCGCA GCTGCAACAA CAGCAGCCCC AACAGCACTT TAGCTCTCCG 
CACCACATGC CGCCAATGCA CCATCAACAC CAGCTGATGA TGCAACAGCA ACATCGCCAC 
AACCAGCGCT CGCCGCTTCA CCATCATCCT GCGGCGCAGC AGCTTCAGCA GCAGCATCAG 
CAACCATCGC ATTCCCCACA GCATCAGCAT GCGAGACTGC AGTCGCCACA ACCTGCTCCA 
GTACAACAAC AACAGCAGCA GCAGCAGCAA CAACAGCAGC AGCAGCAGCA GTTCCTGCAG 
CAACAGGCTG CCGACACGTG GGGCAACATG AACTTTGGCA ATCCACCGAA CAATCAGCAG 
GTTGAGCTCA AGGATAACAA ATTCTATATT GTGGAATCGG CCGAGTTCCT AGGTCTTCCA 
ATGAATGTGC CGCCATCTCC GCAGCAGCAG CAACAGCAAG CAGTAAGCAA ACCCCAACAG 
CAGCAGCAAC AACAACACCC ACAACCGCAA CAGCCAGATC CAACCCTTGC TGGTGATTTG 
ATGAGCTTCC AGCATATGTG GCCGGCGCCT GGTTTTAGCC AGACTTCACA GCAGCAACAG 
CCACAGCAGC AACAACAGCA GCAAGCGGCG CAGCAGCAAC AGCAGGCGCA AAGTCAGGCA 
AACTCAAGTG ATCCCCACAA CTACAACAAC ATCGGCAGCA TACTAACGAA TCTCATTGAC 
ACAAATCCCG CGCCAATGGA GTACAACTTC GATCTGATGC AGCAGCAACA AACGCCGTCA 
GCCCAACAGC AGCAGCAGCA ACAACAACAA CAGCAGGCGG CGCAGCAGCA ACATCACAGT 
GCCGGTGCAT ATGGGAGCGG TTTGATGCGC AGCGGAGGCG GCGTTTACGG AGGCGCCTAC 
GAGCAGCACT TGAGTGACCA GCTGGTCAGC GAGCAGCAGA AACAGAACAG CTTTCAACCC 
TACCACCCAC ATGGCCTGCA GGCGCAGCAA CAGCAGCCAC TGCATCCGCA TCCGCATTTG 
TTACCCCCGC CAGCGCCGCA TAATAATGTC TACGATCGCA CGGGAGTTGG AGTCGGTGTC 
GGAGTGGGCG TAGGCGTGGG AGTGGGTGTG GGCTATCCAA TGCTGGGAGA CGATACCAAG 
AGCGTCCTGC CGCCCATGAT GGGCGGCATG ATGCAGCAAA TGCCACCGCA CGGGCAGCAG 
CCGGATCTAA TCTACTACCC AGTGAAGAAC GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG10543 ( NM_001274183.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261112.1 Drosophila melanogaster uncharacterized protein, transcript variant F (CG10543), mRNA.

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CloneID	OFa12093
Clone ID Related Accession (Same CDS sequence)	NM_001274183.1
Accession Version	NM_001274183.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATAACA AAACTACACA GTTGGATTTT AAATTACTGG TGGATCAATC ACTAAATCTA 
CTGGACAGCC TCAACACGGC ATTGCGGTGC GATGCTATTC AATTCTTGCT GCAGACGCTT 
AAGTGCAAAT ACCCGGAGTC CTGCGACCAA GTCGTCAGGA ATCTGTTCAG TCACATAGCT 
GCGGCCAATG ACAATGGCGA GTCTGGAGGC AGGGCGCAGC AGCACGAGCT AGCGAGGACG 
AAGCAGCTCC AGCTGCTGCT GACCGCCAAA AAGGAGAAGA AAGAGCTGGG AATCGGTTGC 
GAGGCGGAGA TCAAGCGAGA GAACGATGAG GAGGAGGCGG GCTCGACGGA TCTGAACCAA 
AATTTCGAGA AGATACCCTG CAAAGAGGAG TTCCTGTGCC TCGAGGAGGA AGAGGAGGAC 
TTTCTCGACG ATGCGGACAC GCAGAACTCG TCTTCCCTGC AGTTCCACAC CGGTAACAAT 
GTGGACGCCC TGGCCCTGGG CCCTGGCGAT CCGCTGGAGC TGATCCAGTC CGGAGTTTCG 
CTGCTGGTCA AGCGGAAGTA TGCTGCCACC TTCGATGACG AACTGATCGG GGATGGGGAT 
GGTGATGAGG CCAACAGTAA CAGCAGTGAC GGCAAGATGG TCAAGCGAAA GCGCACCAAC 
AACATGCACC TGACCGTGTC GAGTGTGCGA CGGAAAGAGG AGCACGGCCA GCTGGGCGAT 
GGCTATGTCT TCCACGAGGA CAGCCAGTAT ACGATGCGAT ATCACCACAG CACCGGCGAG 
TTCAGTGAGC GGGCCTTTAA GGCCCAGTTC CGGGCTCTGC TCCGCCTGGC GCTCAGTCAG 
CACCACAAGC CCTTTCTCCG CCAGTTCTAC GAGAACTTTG TTGTGAATGG ACGCTTGCTG 
GGGACCACGC CCTCGATGCG TGTCCTGAAG GAGCTGCGCG AACAGCGGCT GGAGGAGTGG 
CGACGTCAGC GTGAACGCCG CCAACTGGTC ATCCTGATGG AGCAGAGCGA GACTCAGCGG 
GACGAAGAGT TTCAGCAGCA GCAGGAGCTG GTGGAGAACC AAGAGGAGGT CCAGCAGGAG 
CAAGAACAGC AACAGCAGCA GCTGGAGGAT ATTCAGCAGC AGCATCTGGA GGAGATTCAG 
CGGCAGCAGC AATTGCCGGC CATCTCGTTT GGCGACTCCG AATTGGAGTC AGAAACGGAA 
TCGCAGCTGT CGTCGGCTGC CCAAGAGATT ATGAAGTTTG AGGAGTTCAT TGACGAGGGT 
GTAGGCGCTG GCCGTCTGAT CGATGGTCTG GAGATGGAAC CCGAGATCGA TGACATGCTA 
GCTGGCGCCG GCAGTATGAA CAGCGCCAGT GGACACAGCA GCAACAGTAG CAGCAGCGGA 
AGTGGGAGCG CCGGTAATGG GAATGGCATC AGCGGGGTGA TCCTGGAGAA CAATAGTCAT 
CATACGCATC ACCTAAGTAG CAGCAGCAGC GCAAATCTTG TGATCAACGG CCTTACGTCA 
AGCAATAGCA TCAGCAACAA TAATAATAAC AGCAACAATG TCAGTTTACA CCGCCCTCAA 
TTGACGCCAC AGGCGCAGAA TCAGCTGGCA GCGTCGATGA AACCATCAGA CCATATTCCC 
ATATCGATGC CCATGGAGAA CATGGATTTG AAAGCCGGGC AGGCGGCTCA TGGGACTGCC 
AACGCCATCA TGCAGGGCGG TAGTTCGAGC CTCGCGAGCC AGCTACATCA GCAGCAAAAA 
CCCTACAACT CAAGCAATAA TCCGCTGTCT ATGATGGGTG GAATGATGCA GCAGCAGCAG 
CAGCAGCATG TTGCTATGCC GCACCACCAG CGCCAGATGA TGATGATGCC AGAGGATTTC 
CCGCAACACG GAGCCTCAAT GATGAACAGT CGTCAGATGC CCGCTCTGGC CGCCTTGTCG 
AATCTGGGCG ACACACCGGC TATGAGTGGC CAGCTTAACT CCTCCCTAGA GCTGGACGAC 
AATGACCTGT CGGCGGATGA GGACGACGAC GATCTGGACC ATGACCTGGA CGAATTAGAC 
GCGGCCAAGC AACAACTAAT AGATGGCGGC AGCAGTAGCA GCACATCGCT TCAGGCGCCA 
CCATCGCAGC ACGGCAGCGG TAGTGGAGGC AGCGGCGGCC AGACGCCCGG TAGCAAAAAG 
GATAAGCCCA GCTACAACTG CCTGCTCTGC CCCAAGTCGT ATCGCAAGCG CAAGTCCTTG 
CTAGACCACT ACAAAATGCA TCCGGGCTAC TGCCATGACT GCGGTCAGCG CAATGGGAAC 
ACGCTGGAGG AAATAATCCA CCACAACCGG ACAATGCATG TCAAGGAGTT TCCGTTTGTG 
TGCGAGACGT GCGGAGAGTC GTACTCACGC AAACAGCAGT TCCACGCCCA CGTGGAGTCG 
CACAATAAGA AGGAAATCAA AACCTTTCCC TGCGGCGAGT GCGGTCTTAA GTTTCCGCAA 
AAGAAACTGC AGCAACACTT CGAGGAGACG GGCCACAAGG CGGACGGCGC TATCTGCGAG 
GTGTGCGGCG AGGAGTTCCA GTCGAAGAAT GCCCTGTACC AGCACATTAT TCGTGTGCAC 
AAGCGGGACA ACTTCTTCGA GTGCCACATT TGCCATAACC GCTTCACGCT GAAAGCCAAC 
CTGGAGCGGC ACGTCCAGCT GCACACCGAG ATCAAACGGC CCTACGTGTG CGATCTGTGC 
GGCTCGTCCT ACTTCACATA TCCCGCGCTC AAGGAGCACT ACAGCAACGC CCACGTGGAC 
GTGTCCGAGT GCAAATGCAC GCTTTGCGGC AAGCGCTTCG GTTCGGCCAA GTCGCTGCAG 
CGACACCTTC CGTCGCACTC GGAGGAAAGG CCGCACTGCT GCAACTATTG CGATCAGACC 
TTCAAGTGGA AAACGCATCT GGTGCGCCAC AAGCAGACAA TGCACGGAAA CGAGCCGCCT 
CCGCCTAAGA AGGGAAAGCA GCGCTTTCCC AAAACAAGCG AAGAAGCAGA TATGGGTAGC 
TTACCGGACA TGCCGGGTCC ACCGCCTGTG AAGGCCAGCA AGAAGGCAGC AACCAGCAAG 
GCGAAAGCGC AAGCAGCGGC CGCCGCAGCC GCAGCAGCAT CATCCCAGCA ACAACAACAG 
AAGGGTTCTG CAGCAACACC GACGCCGCCA CCGCCGGTCT CCACCACACC GGGATGCCTG 
CAACAGGATC CGTTCAATGC GGCCATGGTT AGCAGCAACA GCTCGCAGTC CTCCACGGCG 
TCCACGTCGC AACACTCGGT GTCGACGAGT GAATCTCAGC AGAATTCCAT ATACAATCAG 
AGCTTTAATG CGGAGAAACA GCAGCCAGGA CAGCAGCAGC CCCAGAATAC CTTGCATCAG 
CAACATCCAA CGCCGCCACC TCCGCAGCAG CAGCAACAAC AACAGCAGCA GCAGCAGGCG 
CTGCCACAGT CACGAGTTGG GCCCGGAGAA CTGCATCTGC AGCGGATGAA TAGTTTCGGC 
CAGGACGGAC AGTTTCACTT TGAGTCCAAT CCGGCAGCTG GTGCGTATCA ACAGCATCAA 
CTGATCAGCC AGTATCAGCA ACAACAGCAG CAGCAGCAGC AACAACAGCA GCAGCAGCCC 
CAGCAGCAGC AACAACATCA TCTCGCCCAA CAGCAGCAGC AGCACATGAG GAGTCACACG 
CCCCAGAGTC CACATCCTCC GCATCCTTCG CAGCTGCAAC AACAGCAGCC CCAACAGCAC 
TTTAGCTCTC CGCACCACAT GCCGCCAATG CACCATCAAC ACCAGCTGAT GATGCAACAG 
CAACATCGCC ACAACCAGCG CTCGCCGCTT CACCATCATC CTGCGGCGCA GCAGCTTCAG 
CAGCAGCATC AGCAACCATC GCATTCCCCA CAGCATCAGC ATGCGAGACT GCAGTCGCCA 
CAACCTGCTC CAGTACAACA ACAACAGCAG CAGCAGCAGC AACAACAGCA GCAGCAGCAG 
CAGTTCCTGC AGCAACAGGC TGCCGACACG TGGGGCAACA TGAACTTTGG CAATCCACCG 
AACAATCAGC AGGTTGAGCT CAAGGATAAC AAATTCTATA TTGTGGAATC GGCCGAGTTC 
CTAGGTCTTC CAATGAATGT GCCGCCATCT CCGCAGCAGC AGCAACAGCA AGCAGTAAGC 
AAACCCCAAC AGCAGCAGCA ACAACAACAC CCACAACCGC AACAGCCAGA TCCAACCCTT 
GCTGGTGATT TGATGAGCTT CCAGCATATG TGGCCGGCGC CTGGTTTTAG CCAGACTTCA 
CAGCAGCAAC AGCCACAGCA GCAACAACAG CAGCAAGCGG CGCAGCAGCA ACAGCAGGCG 
CAAAGTCAGG CAAACTCAAG TGATCCCCAC AACTACAACA ACATCGGCAG CATACTAACG 
AATCTCATTG ACACAAATCC CGCGCCAATG GAGTACAACT TCGATCTGAT GCAGCAGCAA 
CAAACGCCGT CAGCCCAACA GCAGCAGCAG CAACAACAAC AACAGCAGGC GGCGCAGCAG 
CAACATCACA GTGCCGGTGC ATATGGGAGC GGTTTGATGC GCAGCGGAGG CGGCGTTTAC 
GGAGGCGCCT ACGAGCAGCA CTTGAGTGAC CAGCTGGTCA GCGAGCAGCA GAAACAGAAC 
AGCTTTCAAC CCTACCACCC ACATGGCCTG CAGGCGCAGC AACAGCAGCC ACTGCATCCG 
CATCCGCATT TGTTACCCCC GCCAGCGCCG CATAATAATG TCTACGATCG CACGGGAGTT 
GGAGTCGGTG TCGGAGTGGG CGTAGGCGTG GGAGTGGGTG TGGGCTATCC AATGCTGGGA 
GACGATACCA AGAGCGTCCT GCCGCCCATG ATGGGCGGCA TGATGCAGCA AATGCCACCG 
CACGGGCAGC AGCCGGATCT AATCTACTAC CCAGTGAAGA ACGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261112.1
CDS	671..5578
Translation	MDNKTTQLDFKLLVDQSLNLLDSLNTALRCDAIQFLLQTLKCKYPESCDQVVRNLFSHIAAANDNGESGGRAQQHELARTKQLQLLLTAKKEKKELGIGCEAEIKRENDEEEAGSTDLNQNFEKIPCKEEFLCLEEEEEDFLDDADTQNSSSLQFHTGNNVDALALGPGDPLELIQSGVSLLVKRKYAATFDDELIGDGDGDEANSNSSDGKMVKRKRTNNMHLTVSSVRRKEEHGQLGDGYVFHEDSQYTMRYHHSTGEFSERAFKAQFRALLRLALSQHHKPFLRQFYENFVVNGRLLGTTPSMRVLKELREQRLEEWRRQRERRQLVILMEQSETQRDEEFQQQQELVENQEEVQQEQEQQQQQLEDIQQQHLEEIQRQQQLPAISFGDSELESETESQLSSAAQEIMKFEEFIDEGVGAGRLIDGLEMEPEIDDMLAGAGSMNSASGHSSNSSSSGSGSAGNGNGISGVILENNSHHTHHLSSSSSANLVINGLTSSNSISNNNNNSNNVSLHRPQLTPQAQNQLAASMKPSDHIPISMPMENMDLKAGQAAHGTANAIMQGGSSSLASQLHQQQKPYNSSNNPLSMMGGMMQQQQQQHVAMPHHQRQMMMMPEDFPQHGASMMNSRQMPALAALSNLGDTPAMSGQLNSSLELDDNDLSADEDDDDLDHDLDELDAAKQQLIDGGSSSSTSLQAPPSQHGSGSGGSGGQTPGSKKDKPSYNCLLCPKSYRKRKSLLDHYKMHPGYCHDCGQRNGNTLEEIIHHNRTMHVKEFPFVCETCGESYSRKQQFHAHVESHNKKEIKTFPCGECGLKFPQKKLQQHFEETGHKADGAICEVCGEEFQSKNALYQHIIRVHKRDNFFECHICHNRFTLKANLERHVQLHTEIKRPYVCDLCGSSYFTYPALKEHYSNAHVDVSECKCTLCGKRFGSAKSLQRHLPSHSEERPHCCNYCDQTFKWKTHLVRHKQTMHGNEPPPPKKGKQRFPKTSEEADMGSLPDMPGPPPVKASKKAATSKAKAQAAAAAAAAASSQQQQQKGSAATPTPPPPVSTTPGCLQQDPFNAAMVSSNSSQSSTASTSQHSVSTSESQQNSIYNQSFNAEKQQPGQQQPQNTLHQQHPTPPPPQQQQQQQQQQQALPQSRVGPGELHLQRMNSFGQDGQFHFESNPAAGAYQQHQLISQYQQQQQQQQQQQQQQPQQQQQHHLAQQQQQHMRSHTPQSPHPPHPSQLQQQQPQQHFSSPHHMPPMHHQHQLMMQQQHRHNQRSPLHHHPAAQQLQQQHQQPSHSPQHQHARLQSPQPAPVQQQQQQQQQQQQQQQQFLQQQAADTWGNMNFGNPPNNQQVELKDNKFYIVESAEFLGLPMNVPPSPQQQQQQAVSKPQQQQQQQHPQPQQPDPTLAGDLMSFQHMWPAPGFSQTSQQQQPQQQQQQQAAQQQQQAQSQANSSDPHNYNNIGSILTNLIDTNPAPMEYNFDLMQQQQTPSAQQQQQQQQQQQAAQQQHHSAGAYGSGLMRSGGGVYGGAYEQHLSDQLVSEQQKQNSFQPYHPHGLQAQQQQPLHPHPHLLPPPAPHNNVYDRTGVGVGVGVGVGVGVGVGYPMLGDDTKSVLPPMMGGMMQQMPPHGQQPDLIYYPVKND

Target ORF information:

RefSeq Version	NM_001274183.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant F (CG10543), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274183.1

ORF Insert Sequence:

ATGGATAACA AAACTACACA GTTGGATTTT AAATTACTGG TGGATCAATC ACTAAATCTA 
CTGGACAGCC TCAACACGGC ATTGCGGTGC GATGCTATTC AATTCTTGCT GCAGACGCTT 
AAGTGCAAAT ACCCGGAGTC CTGCGACCAA GTCGTCAGGA ATCTGTTCAG TCACATAGCT 
GCGGCCAATG ACAATGGCGA GTCTGGAGGC AGGGCGCAGC AGCACGAGCT AGCGAGGACG 
AAGCAGCTCC AGCTGCTGCT GACCGCCAAA AAGGAGAAGA AAGAGCTGGG AATCGGTTGC 
GAGGCGGAGA TCAAGCGAGA GAACGATGAG GAGGAGGCGG GCTCGACGGA TCTGAACCAA 
AATTTCGAGA AGATACCCTG CAAAGAGGAG TTCCTGTGCC TCGAGGAGGA AGAGGAGGAC 
TTTCTCGACG ATGCGGACAC GCAGAACTCG TCTTCCCTGC AGTTCCACAC CGGTAACAAT 
GTGGACGCCC TGGCCCTGGG CCCTGGCGAT CCGCTGGAGC TGATCCAGTC CGGAGTTTCG 
CTGCTGGTCA AGCGGAAGTA TGCTGCCACC TTCGATGACG AACTGATCGG GGATGGGGAT 
GGTGATGAGG CCAACAGTAA CAGCAGTGAC GGCAAGATGG TCAAGCGAAA GCGCACCAAC 
AACATGCACC TGACCGTGTC GAGTGTGCGA CGGAAAGAGG AGCACGGCCA GCTGGGCGAT 
GGCTATGTCT TCCACGAGGA CAGCCAGTAT ACGATGCGAT ATCACCACAG CACCGGCGAG 
TTCAGTGAGC GGGCCTTTAA GGCCCAGTTC CGGGCTCTGC TCCGCCTGGC GCTCAGTCAG 
CACCACAAGC CCTTTCTCCG CCAGTTCTAC GAGAACTTTG TTGTGAATGG ACGCTTGCTG 
GGGACCACGC CCTCGATGCG TGTCCTGAAG GAGCTGCGCG AACAGCGGCT GGAGGAGTGG 
CGACGTCAGC GTGAACGCCG CCAACTGGTC ATCCTGATGG AGCAGAGCGA GACTCAGCGG 
GACGAAGAGT TTCAGCAGCA GCAGGAGCTG GTGGAGAACC AAGAGGAGGT CCAGCAGGAG 
CAAGAACAGC AACAGCAGCA GCTGGAGGAT ATTCAGCAGC AGCATCTGGA GGAGATTCAG 
CGGCAGCAGC AATTGCCGGC CATCTCGTTT GGCGACTCCG AATTGGAGTC AGAAACGGAA 
TCGCAGCTGT CGTCGGCTGC CCAAGAGATT ATGAAGTTTG AGGAGTTCAT TGACGAGGGT 
GTAGGCGCTG GCCGTCTGAT CGATGGTCTG GAGATGGAAC CCGAGATCGA TGACATGCTA 
GCTGGCGCCG GCAGTATGAA CAGCGCCAGT GGACACAGCA GCAACAGTAG CAGCAGCGGA 
AGTGGGAGCG CCGGTAATGG GAATGGCATC AGCGGGGTGA TCCTGGAGAA CAATAGTCAT 
CATACGCATC ACCTAAGTAG CAGCAGCAGC GCAAATCTTG TGATCAACGG CCTTACGTCA 
AGCAATAGCA TCAGCAACAA TAATAATAAC AGCAACAATG TCAGTTTACA CCGCCCTCAA 
TTGACGCCAC AGGCGCAGAA TCAGCTGGCA GCGTCGATGA AACCATCAGA CCATATTCCC 
ATATCGATGC CCATGGAGAA CATGGATTTG AAAGCCGGGC AGGCGGCTCA TGGGACTGCC 
AACGCCATCA TGCAGGGCGG TAGTTCGAGC CTCGCGAGCC AGCTACATCA GCAGCAAAAA 
CCCTACAACT CAAGCAATAA TCCGCTGTCT ATGATGGGTG GAATGATGCA GCAGCAGCAG 
CAGCAGCATG TTGCTATGCC GCACCACCAG CGCCAGATGA TGATGATGCC AGAGGATTTC 
CCGCAACACG GAGCCTCAAT GATGAACAGT CGTCAGATGC CCGCTCTGGC CGCCTTGTCG 
AATCTGGGCG ACACACCGGC TATGAGTGGC CAGCTTAACT CCTCCCTAGA GCTGGACGAC 
AATGACCTGT CGGCGGATGA GGACGACGAC GATCTGGACC ATGACCTGGA CGAATTAGAC 
GCGGCCAAGC AACAACTAAT AGATGGCGGC AGCAGTAGCA GCACATCGCT TCAGGCGCCA 
CCATCGCAGC ACGGCAGCGG TAGTGGAGGC AGCGGCGGCC AGACGCCCGG TAGCAAAAAG 
GATAAGCCCA GCTACAACTG CCTGCTCTGC CCCAAGTCGT ATCGCAAGCG CAAGTCCTTG 
CTAGACCACT ACAAAATGCA TCCGGGCTAC TGCCATGACT GCGGTCAGCG CAATGGGAAC 
ACGCTGGAGG AAATAATCCA CCACAACCGG ACAATGCATG TCAAGGAGTT TCCGTTTGTG 
TGCGAGACGT GCGGAGAGTC GTACTCACGC AAACAGCAGT TCCACGCCCA CGTGGAGTCG 
CACAATAAGA AGGAAATCAA AACCTTTCCC TGCGGCGAGT GCGGTCTTAA GTTTCCGCAA 
AAGAAACTGC AGCAACACTT CGAGGAGACG GGCCACAAGG CGGACGGCGC TATCTGCGAG 
GTGTGCGGCG AGGAGTTCCA GTCGAAGAAT GCCCTGTACC AGCACATTAT TCGTGTGCAC 
AAGCGGGACA ACTTCTTCGA GTGCCACATT TGCCATAACC GCTTCACGCT GAAAGCCAAC 
CTGGAGCGGC ACGTCCAGCT GCACACCGAG ATCAAACGGC CCTACGTGTG CGATCTGTGC 
GGCTCGTCCT ACTTCACATA TCCCGCGCTC AAGGAGCACT ACAGCAACGC CCACGTGGAC 
GTGTCCGAGT GCAAATGCAC GCTTTGCGGC AAGCGCTTCG GTTCGGCCAA GTCGCTGCAG 
CGACACCTTC CGTCGCACTC GGAGGAAAGG CCGCACTGCT GCAACTATTG CGATCAGACC 
TTCAAGTGGA AAACGCATCT GGTGCGCCAC AAGCAGACAA TGCACGGAAA CGAGCCGCCT 
CCGCCTAAGA AGGGAAAGCA GCGCTTTCCC AAAACAAGCG AAGAAGCAGA TATGGGTAGC 
TTACCGGACA TGCCGGGTCC ACCGCCTGTG AAGGCCAGCA AGAAGGCAGC AACCAGCAAG 
GCGAAAGCGC AAGCAGCGGC CGCCGCAGCC GCAGCAGCAT CATCCCAGCA ACAACAACAG 
AAGGGTTCTG CAGCAACACC GACGCCGCCA CCGCCGGTCT CCACCACACC GGGATGCCTG 
CAACAGGATC CGTTCAATGC GGCCATGGTT AGCAGCAACA GCTCGCAGTC CTCCACGGCG 
TCCACGTCGC AACACTCGGT GTCGACGAGT GAATCTCAGC AGAATTCCAT ATACAATCAG 
AGCTTTAATG CGGAGAAACA GCAGCCAGGA CAGCAGCAGC CCCAGAATAC CTTGCATCAG 
CAACATCCAA CGCCGCCACC TCCGCAGCAG CAGCAACAAC AACAGCAGCA GCAGCAGGCG 
CTGCCACAGT CACGAGTTGG GCCCGGAGAA CTGCATCTGC AGCGGATGAA TAGTTTCGGC 
CAGGACGGAC AGTTTCACTT TGAGTCCAAT CCGGCAGCTG GTGCGTATCA ACAGCATCAA 
CTGATCAGCC AGTATCAGCA ACAACAGCAG CAGCAGCAGC AACAACAGCA GCAGCAGCCC 
CAGCAGCAGC AACAACATCA TCTCGCCCAA CAGCAGCAGC AGCACATGAG GAGTCACACG 
CCCCAGAGTC CACATCCTCC GCATCCTTCG CAGCTGCAAC AACAGCAGCC CCAACAGCAC 
TTTAGCTCTC CGCACCACAT GCCGCCAATG CACCATCAAC ACCAGCTGAT GATGCAACAG 
CAACATCGCC ACAACCAGCG CTCGCCGCTT CACCATCATC CTGCGGCGCA GCAGCTTCAG 
CAGCAGCATC AGCAACCATC GCATTCCCCA CAGCATCAGC ATGCGAGACT GCAGTCGCCA 
CAACCTGCTC CAGTACAACA ACAACAGCAG CAGCAGCAGC AACAACAGCA GCAGCAGCAG 
CAGTTCCTGC AGCAACAGGC TGCCGACACG TGGGGCAACA TGAACTTTGG CAATCCACCG 
AACAATCAGC AGGTTGAGCT CAAGGATAAC AAATTCTATA TTGTGGAATC GGCCGAGTTC 
CTAGGTCTTC CAATGAATGT GCCGCCATCT CCGCAGCAGC AGCAACAGCA AGCAGTAAGC 
AAACCCCAAC AGCAGCAGCA ACAACAACAC CCACAACCGC AACAGCCAGA TCCAACCCTT 
GCTGGTGATT TGATGAGCTT CCAGCATATG TGGCCGGCGC CTGGTTTTAG CCAGACTTCA 
CAGCAGCAAC AGCCACAGCA GCAACAACAG CAGCAAGCGG CGCAGCAGCA ACAGCAGGCG 
CAAAGTCAGG CAAACTCAAG TGATCCCCAC AACTACAACA ACATCGGCAG CATACTAACG 
AATCTCATTG ACACAAATCC CGCGCCAATG GAGTACAACT TCGATCTGAT GCAGCAGCAA 
CAAACGCCGT CAGCCCAACA GCAGCAGCAG CAACAACAAC AACAGCAGGC GGCGCAGCAG 
CAACATCACA GTGCCGGTGC ATATGGGAGC GGTTTGATGC GCAGCGGAGG CGGCGTTTAC 
GGAGGCGCCT ACGAGCAGCA CTTGAGTGAC CAGCTGGTCA GCGAGCAGCA GAAACAGAAC 
AGCTTTCAAC CCTACCACCC ACATGGCCTG CAGGCGCAGC AACAGCAGCC ACTGCATCCG 
CATCCGCATT TGTTACCCCC GCCAGCGCCG CATAATAATG TCTACGATCG CACGGGAGTT 
GGAGTCGGTG TCGGAGTGGG CGTAGGCGTG GGAGTGGGTG TGGGCTATCC AATGCTGGGA 
GACGATACCA AGAGCGTCCT GCCGCCCATG ATGGGCGGCA TGATGCAGCA AATGCCACCG 
CACGGGCAGC AGCCGGATCT AATCTACTAC CCAGTGAAGA ACGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG10543 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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