CG3216 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG3216
Entrez Gene ID	37376
Full Name	CG3216 gene product from transcript CG3216-RD
Synonyms	Dmel\CG3216,GYC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 57B5-57B5

mRNA and Protein(s)

mRNA	Protein	Name
NM_166415.2	NP_726013.2	uncharacterized protein, isoform C
NM_137688.2	NP_611532.2	uncharacterized protein, isoform D

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NPR1	XP_003308543.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008691	XP_314790.4
Canis lupus familiaris (dog)	NPR1	XP_547577.2
Danio rerio (zebrafish)	npr1a	NP_001038402.1
	NPR1	XP_002808282.1
Mus musculus (house mouse)	Npr1	NP_032753.5
Homo sapiens (human)	NPR1	NP_000897.3
Bos taurus (cattle)	NPR1	NP_001179680.1
Rattus norvegicus (Norway rat)	Npr1	NP_036745.1
Drosophila melanogaster (fruit fly)	CG3216	NP_726013.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG3216 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG3216 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa10726	NM_137688.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant D (CG3216), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa10727	NM_166415.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG3216), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG3216 ( NM_137688.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_611532.2 Drosophila melanogaster uncharacterized protein, transcript variant D (CG3216), partial mRNA.

Price & Availability↑

CloneID	OFa10726
Clone ID Related Accession (Same CDS sequence)	NM_137688.2
Accession Version	NM_137688.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 26, 2009 this sequence version replaced NM_137688.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCATCTGC TTGGGATCAG CATCCACTTC TTCTTCCTGA TGTACGTCAA CTGCTTTAGT 
GCCCATCCGA ATCCCAGGCG CAATGATATA ACCTGGGATG ATCTGAACAA GGATATTAGT 
TTGGACTCCA CTACTTCCTT GGCGGGGCTC AATGCCAGTG ATGCGGGCCT AGAGCAAAGA 
ATGTACGAGA GGAGCAGGGA ATCCAAGTCT ACGCAGCTTT CTAGATACAC TGAGGTGGGC 
GAAATGGGCT CCACAATGCG TGTCTATAAC GTTGGCGTTC TGATGGCTTC TCATTTGGAT 
TCTCCATTCG ATCTGGAGCG TTGTGGACCG GCCGTTGACT TGGCCTTGGA TGAGATTAAC 
AAGGTTTTCC TGAAGCCTCA CAACATAACA CTACTGAAAA AGAAGGGAAG CTATCCATCC 
TGTTCGGGTG CTCGGGCTCC TGGACTGGCT GCGGACATGT ACTTCCAGGA CGACGTAATC 
GCGTTTATTG GACCAGCCTG CGCCTTTGCC CTTGAGCCGG TGGCACGTTT GGCCGCCTAT 
TGGAACAAGC CCATCATCAC CGGCATGGGA GATCAGCCGC CTTCCTCGGA AGGTGAGCTG 
ACGGTCACTT CCGGAATCCT GGGAAGGATA CACAAGTGGA AGAATGAGAA TACGGGCATG 
TTCAAGGACA AGTCCAAGTA CCCGACGCTC ACCCGGATGT CCTACTGCCA GTGCCGCCTC 
ATCCTGGTCT TTGCCAGCGT CATTCGGCAG TTCAACTGGA ATCACGTTGC CCTGCTGGTG 
GACAGATCGG AGCTCTTCTC GTGGACGGTG GGCAAGAATC TGGAGTACGG TCTGCGGCAG 
GAGGGACTCT TGAGCTTCGT CAAGGAGCTC AACGGCAACG AGGAGGAGGT GTACGAAAAC 
TATCTAAAGG ACGCCAGCAT GTATGCCAGA GTGGTCATCC TGTCGGTTCG CGGCGTTCTT 
GTGCGAAAAT TCATGTTGGC AGCCCACAGC CTGGGCATGA CCAATGGCGA GTGGGTGTTC 
CTGGACGTGG AAATCTTTCA GAGCGAATAC TGGGGCGACA AGGGTTGGGA GATGAAGGAT 
GAGCACGATG CCAAGGCGCG GAAGGCATAC GAAGCCCTCC TGAGAGTCAG TCTCCTGCAG 
CCGACAAGTC CCAAGTTCCA GGACTTTGCC GACAATGTGA GGGAGAATGC GCTCTACGAT 
TACAACTACA CGTTTGGCGA GGGCGAGGAG GTGAACTTCT TTATTGGAGC CTTCTACGAT 
GGCGTCTACC TGCTGGGAAT GGCCTTGAAC GAGACCCTCA CCGAGGGTGG TGACATCCGG 
GATGGGGTCA ACATCACCAG GCGGATGTGG AATCGCACCT TCGAAGGTAT CACGGGGCAC 
GTGCGCATCG ACGACAACGG TGACCGGGAT GCTGATTACT CCATTCTCGA TCTGGATCCC 
ATCAATGGAA AGTTCTCCGT TGTGGCCCAT TATTCCGGTG TTCACAAGCA GATGAAGCTC 
AGCAAGGAGC TGAATAATAT GTCCTGGCGC GTACGACCCG ATGATGTCCT CATCGAGATG 
GGCGGCATGT TTGGTTCGAA GGGAGGCTTG CAGCGCCTGG ATGTGGAGAA CATATCGCTG 
CAGCAGTTCG GTATCCATTC GGGACGCGCA TCGATAGCCA GCTTCACGTC GCTGCCGCCG 
CAAGTGTACA CCACCATTGG CCAGTTCAAG GGCGAACGGG TGGCCATCAA GAAGGTGAAC 
GTGAAGAAGG TGGACCTGAC GCCGCAGCTC CTTTGGGAGA TCAAGCAGGC GCGGGATGTG 
AGCCACGAGA ATACGGTGCG TTTCGTGGGC GCCTGCATCG ATCTGCCGCG ACCCACCGTG 
CTCATCCTCA CCGAATATTG CTCGAGGGGC AGTCTGAAGG ATGTGCTGGA GAACGAGGCC 
ATCGAGTTGG ACTGGAACTT TCGCATGTCT CTCATCCACG ACATTGTCAA GGGCATGAAC 
TATCTGCACA ATAGCGATGT GGCTGCCCAT GGAAAGCTGA GGTCTTGCAA TTGCCTGATA 
GACGGACGAT TCGTTCTCAA GATCTCCGAC TTTGGACTGA GAACGCTGAC CACGCCCTCT 
GATTTTGTGC GGGATCAGAA CTACTACCTC AAGCTCTTGT GGATTGCTCC CGAACTGCTT 
CCACTGACCA CCATACCTGG TTGCTGTCCA GCCACGCAGC GCGGCGATGT GTACTCCTTT 
GGCATTATTC TGGAGGAGAT CGTCAACCGT GGTGGACCCT ACCAGGAGGC GCGCCAGCAG 
ATGGACGTTC ACACGATCCT GCACAAGGTG CGCCAGTGCA ATGGATTCCG TCCGCTCATC 
AGAGAACGCG AGTGTCCACC TGATCTGCTG GAGCTAATGG AGAAGTGCTG GGCAGACAAT 
CAGGAGGAGC GGCCCACTTT CTCCACCATA CGCAGTAATA TTCGCACCAT AATGAAAGGC 
TTCTGTGAGA ATCTAATGGA TGATCTACTG AATCGCATGG AGCAGTATGC CAACAATCTC 
GAGAGTCTCG TGGAGGAGAA AACCCGCCAG TTGAGCCTGG AAAAGCAGCG TACGGAGGAG 
CTGCTCTACC AAATCCTGCC GCGTCCTGTG GCCCAGCAAC TGATGGCCGG CGATCTTGTG 
GAGCCGGAGG AGTTCAGCAG CGTGACCATA TACTTTAGTG ACATCGTTGG CTTCACCGAA 
CTGTGCGCCC GTAGCAGTCC CATGGACGTG GTCAACTTCC TGAATGACTT GTATAGCACA 
TTTGACCGAA TCATTGGCTT CTACGACGTC TACAAAGTGG AAACGATTGG TGATGCCTAT 
CTGGTGGTTT CTGGATTGCC GGAGCCCAAT GGTGACAAGC ATGCCCGCGA GATTGCTCTA 
ATGGCCCTGG ACATCCTACG AGCAGTCAGC TCCTTCAATC TCCGCCATAA ACCGGAATAC 
AAGATACAGA TTCGCATTGG GATGCATTCG GGTTCCGTGT GCGCCGGAGT CGTGGGCAAG 
AAGATGCCGC ACTACTGTTT GTTTGGCGAC ACGGTCAACA CGGCGTCGAG AATGGAGAGC 
ACTGGCCAGC CGGGCAAGAT ACATGTGTCC TCGGCCACCA AGGCCATCCT GGACAAGTTC 
GGCACCTTCC AAATGGAGCA GCGCGGCGAT GTCGAGCTAA AGGGCAAGGG CACGGTCACC 
ACCTACTGGC TGAATAGCAC CTCCGAGGGC GAGGCGCGTC CTCCGACTCC GCAGATCCTG 
ACCACCGATG AGGTGCCCTT CCCGCTGCTC TTCGCCGGCA TGGGAAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_611532.2
CDS	1..3291
Translation	MHLLGISIHFFFLMYVNCFSAHPNPRRNDITWDDLNKDISLDSTTSLAGLNASDAGLEQRMYERSRESKSTQLSRYTEVGEMGSTMRVYNVGVLMASHLDSPFDLERCGPAVDLALDEINKVFLKPHNITLLKKKGSYPSCSGARAPGLAADMYFQDDVIAFIGPACAFALEPVARLAAYWNKPIITGMGDQPPSSEGELTVTSGILGRIHKWKNENTGMFKDKSKYPTLTRMSYCQCRLILVFASVIRQFNWNHVALLVDRSELFSWTVGKNLEYGLRQEGLLSFVKELNGNEEEVYENYLKDASMYARVVILSVRGVLVRKFMLAAHSLGMTNGEWVFLDVEIFQSEYWGDKGWEMKDEHDAKARKAYEALLRVSLLQPTSPKFQDFADNVRENALYDYNYTFGEGEEVNFFIGAFYDGVYLLGMALNETLTEGGDIRDGVNITRRMWNRTFEGITGHVRIDDNGDRDADYSILDLDPINGKFSVVAHYSGVHKQMKLSKELNNMSWRVRPDDVLIEMGGMFGSKGGLQRLDVENISLQQFGIHSGRASIASFTSLPPQVYTTIGQFKGERVAIKKVNVKKVDLTPQLLWEIKQARDVSHENTVRFVGACIDLPRPTVLILTEYCSRGSLKDVLENEAIELDWNFRMSLIHDIVKGMNYLHNSDVAAHGKLRSCNCLIDGRFVLKISDFGLRTLTTPSDFVRDQNYYLKLLWIAPELLPLTTIPGCCPATQRGDVYSFGIILEEIVNRGGPYQEARQQMDVHTILHKVRQCNGFRPLIRERECPPDLLELMEKCWADNQEERPTFSTIRSNIRTIMKGFCENLMDDLLNRMEQYANNLESLVEEKTRQLSLEKQRTEELLYQILPRPVAQQLMAGDLVEPEEFSSVTIYFSDIVGFTELCARSSPMDVVNFLNDLYSTFDRIIGFYDVYKVETIGDAYLVVSGLPEPNGDKHAREIALMALDILRAVSSFNLRHKPEYKIQIRIGMHSGSVCAGVVGKKMPHYCLFGDTVNTASRMESTGQPGKIHVSSATKAILDKFGTFQMEQRGDVELKGKGTVTTYWLNSTSEGEARPPTPQILTTDEVPFPLLFAGMGK

Target ORF information:

RefSeq Version	NM_137688.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant D (CG3216), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137688.2

ORF Insert Sequence:

ATGCATCTGC TTGGGATCAG CATCCACTTC TTCTTCCTGA TGTACGTCAA CTGCTTTAGT 
GCCCATCCGA ATCCCAGGCG CAATGATATA ACCTGGGATG ATCTGAACAA GGATATTAGT 
TTGGACTCCA CTACTTCCTT GGCGGGGCTC AATGCCAGTG ATGCGGGCCT AGAGCAAAGA 
ATGTACGAGA GGAGCAGGGA ATCCAAGTCT ACGCAGCTTT CTAGATACAC TGAGGTGGGC 
GAAATGGGCT CCACAATGCG TGTCTATAAC GTTGGCGTTC TGATGGCTTC TCATTTGGAT 
TCTCCATTCG ATCTGGAGCG TTGTGGACCG GCCGTTGACT TGGCCTTGGA TGAGATTAAC 
AAGGTTTTCC TGAAGCCTCA CAACATAACA CTACTGAAAA AGAAGGGAAG CTATCCATCC 
TGTTCGGGTG CTCGGGCTCC TGGACTGGCT GCGGACATGT ACTTCCAGGA CGACGTAATC 
GCGTTTATTG GACCAGCCTG CGCCTTTGCC CTTGAGCCGG TGGCACGTTT GGCCGCCTAT 
TGGAACAAGC CCATCATCAC CGGCATGGGA GATCAGCCGC CTTCCTCGGA AGGTGAGCTG 
ACGGTCACTT CCGGAATCCT GGGAAGGATA CACAAGTGGA AGAATGAGAA TACGGGCATG 
TTCAAGGACA AGTCCAAGTA CCCGACGCTC ACCCGGATGT CCTACTGCCA GTGCCGCCTC 
ATCCTGGTCT TTGCCAGCGT CATTCGGCAG TTCAACTGGA ATCACGTTGC CCTGCTGGTG 
GACAGATCGG AGCTCTTCTC GTGGACGGTG GGCAAGAATC TGGAGTACGG TCTGCGGCAG 
GAGGGACTCT TGAGCTTCGT CAAGGAGCTC AACGGCAACG AGGAGGAGGT GTACGAAAAC 
TATCTAAAGG ACGCCAGCAT GTATGCCAGA GTGGTCATCC TGTCGGTTCG CGGCGTTCTT 
GTGCGAAAAT TCATGTTGGC AGCCCACAGC CTGGGCATGA CCAATGGCGA GTGGGTGTTC 
CTGGACGTGG AAATCTTTCA GAGCGAATAC TGGGGCGACA AGGGTTGGGA GATGAAGGAT 
GAGCACGATG CCAAGGCGCG GAAGGCATAC GAAGCCCTCC TGAGAGTCAG TCTCCTGCAG 
CCGACAAGTC CCAAGTTCCA GGACTTTGCC GACAATGTGA GGGAGAATGC GCTCTACGAT 
TACAACTACA CGTTTGGCGA GGGCGAGGAG GTGAACTTCT TTATTGGAGC CTTCTACGAT 
GGCGTCTACC TGCTGGGAAT GGCCTTGAAC GAGACCCTCA CCGAGGGTGG TGACATCCGG 
GATGGGGTCA ACATCACCAG GCGGATGTGG AATCGCACCT TCGAAGGTAT CACGGGGCAC 
GTGCGCATCG ACGACAACGG TGACCGGGAT GCTGATTACT CCATTCTCGA TCTGGATCCC 
ATCAATGGAA AGTTCTCCGT TGTGGCCCAT TATTCCGGTG TTCACAAGCA GATGAAGCTC 
AGCAAGGAGC TGAATAATAT GTCCTGGCGC GTACGACCCG ATGATGTCCT CATCGAGATG 
GGCGGCATGT TTGGTTCGAA GGGAGGCTTG CAGCGCCTGG ATGTGGAGAA CATATCGCTG 
CAGCAGTTCG GTATCCATTC GGGACGCGCA TCGATAGCCA GCTTCACGTC GCTGCCGCCG 
CAAGTGTACA CCACCATTGG CCAGTTCAAG GGCGAACGGG TGGCCATCAA GAAGGTGAAC 
GTGAAGAAGG TGGACCTGAC GCCGCAGCTC CTTTGGGAGA TCAAGCAGGC GCGGGATGTG 
AGCCACGAGA ATACGGTGCG TTTCGTGGGC GCCTGCATCG ATCTGCCGCG ACCCACCGTG 
CTCATCCTCA CCGAATATTG CTCGAGGGGC AGTCTGAAGG ATGTGCTGGA GAACGAGGCC 
ATCGAGTTGG ACTGGAACTT TCGCATGTCT CTCATCCACG ACATTGTCAA GGGCATGAAC 
TATCTGCACA ATAGCGATGT GGCTGCCCAT GGAAAGCTGA GGTCTTGCAA TTGCCTGATA 
GACGGACGAT TCGTTCTCAA GATCTCCGAC TTTGGACTGA GAACGCTGAC CACGCCCTCT 
GATTTTGTGC GGGATCAGAA CTACTACCTC AAGCTCTTGT GGATTGCTCC CGAACTGCTT 
CCACTGACCA CCATACCTGG TTGCTGTCCA GCCACGCAGC GCGGCGATGT GTACTCCTTT 
GGCATTATTC TGGAGGAGAT CGTCAACCGT GGTGGACCCT ACCAGGAGGC GCGCCAGCAG 
ATGGACGTTC ACACGATCCT GCACAAGGTG CGCCAGTGCA ATGGATTCCG TCCGCTCATC 
AGAGAACGCG AGTGTCCACC TGATCTGCTG GAGCTAATGG AGAAGTGCTG GGCAGACAAT 
CAGGAGGAGC GGCCCACTTT CTCCACCATA CGCAGTAATA TTCGCACCAT AATGAAAGGC 
TTCTGTGAGA ATCTAATGGA TGATCTACTG AATCGCATGG AGCAGTATGC CAACAATCTC 
GAGAGTCTCG TGGAGGAGAA AACCCGCCAG TTGAGCCTGG AAAAGCAGCG TACGGAGGAG 
CTGCTCTACC AAATCCTGCC GCGTCCTGTG GCCCAGCAAC TGATGGCCGG CGATCTTGTG 
GAGCCGGAGG AGTTCAGCAG CGTGACCATA TACTTTAGTG ACATCGTTGG CTTCACCGAA 
CTGTGCGCCC GTAGCAGTCC CATGGACGTG GTCAACTTCC TGAATGACTT GTATAGCACA 
TTTGACCGAA TCATTGGCTT CTACGACGTC TACAAAGTGG AAACGATTGG TGATGCCTAT 
CTGGTGGTTT CTGGATTGCC GGAGCCCAAT GGTGACAAGC ATGCCCGCGA GATTGCTCTA 
ATGGCCCTGG ACATCCTACG AGCAGTCAGC TCCTTCAATC TCCGCCATAA ACCGGAATAC 
AAGATACAGA TTCGCATTGG GATGCATTCG GGTTCCGTGT GCGCCGGAGT CGTGGGCAAG 
AAGATGCCGC ACTACTGTTT GTTTGGCGAC ACGGTCAACA CGGCGTCGAG AATGGAGAGC 
ACTGGCCAGC CGGGCAAGAT ACATGTGTCC TCGGCCACCA AGGCCATCCT GGACAAGTTC 
GGCACCTTCC AAATGGAGCA GCGCGGCGAT GTCGAGCTAA AGGGCAAGGG CACGGTCACC 
ACCTACTGGC TGAATAGCAC CTCCGAGGGC GAGGCGCGTC CTCCGACTCC GCAGATCCTG 
ACCACCGATG AGGTGCCCTT CCCGCTGCTC TTCGCCGGCA TGGGAAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG3216 ( NM_166415.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726013.2 Drosophila melanogaster uncharacterized protein, transcript variant C (CG3216), partial mRNA.

Price & Availability↑

CloneID	OFa10727
Clone ID Related Accession (Same CDS sequence)	NM_166415.2
Accession Version	NM_166415.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 26, 2009 this sequence version replaced NM_166415.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCATCTGC TTGGGATCAG CATCCACTTC TTCTTCCTGA TGTACGTCAA CTGCTTTAGT 
GCCCATCCGA ATCCCAGGCG CAATGATATA ACCTGGGATG ATCTGAACAA GGATATTAGT 
TTGGACTCCA CTACTTCCTT GGCGGGGCTC AATGCCAGTG ATGCGGGCCT AGAGCAAAGA 
ATGTACGAGA GGAGCAGGGA ATCCAAGTCT ACGCAGCTTT CTAGATACAC TGAGGTGGGC 
GAAATGGGCT CCACAATGCG TGTCTATAAC GTTGGCGTTC TGATGGCTTC TCATTTGGAT 
TCTCCATTCG ATCTGGAGCG TTGTGGACCG GCCGTTGACT TGGCCTTGGA TGAGATTAAC 
AAGGTTTTCC TGAAGCCTCA CAACATAACA CTACTGAAAA AGAAGGGAAG CTATCCATCC 
TGTTCGGGTG CTCGGGCTCC TGGACTGGCT GCGGACATGT ACTTCCAGGA CGACGTAATC 
GCGTTTATTG GACCAGCCTG CGCCTTTGCC CTTGAGCCGG TGGCACGTTT GGCCGCCTAT 
TGGAACAAGC CCATCATCAC CGGCATGGGA GATCAGCCGC CTTCCTCGGA AGGTGAGCTG 
ACGGTCACTT CCGGAATCCT GGGAAGGATA CACAAGTGGA AGAATGAGAA TACGGGCATG 
TTCAAGGACA AGTCCAAGTA CCCGACGCTC ACCCGGATGT CCTACTGCCA GTGCCGCCTC 
ATCCTGGTCT TTGCCAGCGT CATTCGGCAG TTCAACTGGA ATCACGTTGC CCTGCTGGTG 
GACAGATCGG AGCTCTTCTC GTGGACGGTG GGCAAGAATC TGGAGTACGG TCTGCGGCAG 
GAGGGACTCT TGAGCTTCGT CAAGGAGCTC AACGGCAACG AGGAGGAGGT GTACGAAAAC 
TATCTAAAGG ACGCCAGCAT GTATGCCAGA GTGGTCATCC TGTCGGTTCG CGGCGTTCTT 
GTGCGAAAAT TCATGTTGGC AGCCCACAGC CTGGGCATGA CCAATGGCGA GTGGGTGTTC 
CTGGACGTGG AAATCTTTCA GAGCGAATAC TGGGGCGACA AGGGTTGGGA GATGAAGGAT 
GAGCACGATG CCAAGGCGCG GAAGGCATAC GAAGCCCTCC TGAGAGTCAG TCTCCTGCAG 
CCGACAAGTC CCAAGTTCCA GGACTTTGCC GACAATGTGA GGGAGAATGC GCTCTACGAT 
TACAACTACA CGTTTGGCGA GGGCGAGGAG GTGAACTTCT TTATTGGAGC CTTCTACGAT 
GGCGTCTACC TGCTGGGAAT GGCCTTGAAC GAGACCCTCA CCGAGGGTGG TGACATCCGG 
GATGGGGTCA ACATCACCAG GCGGATGTGG AATCGCACCT TCGAAGGTAT CACGGGGCAC 
GTGCGCATCG ACGACAACGG TGACCGGGAT GCTGATTACT CCATTCTCGA TCTGGATCCC 
ATCAATGGAA AGTTCTCCGT TGTGGCCCAT TATTCCGGTG TTCACAAAGT ATATTCGGCT 
GTTCATGGCA AGAAGATACA TTGGCCTGGT GGTCGTGAGG AGCCACCGCC GGATGTGCCA 
CCCTGTGGCT TCCTGGGCAA TTCTACAGAC TGTGTCGGAA ACTTTTCTAC CATTATGTAC 
TCCGTGTTTG GATTGCTTCT TTTTCTGGGC CTTGTTTTTC TGGTCATCTG TCTTCTGCAA 
AAGCAGATGA AGCTCAGCAA GGAGCTGAAT AATATGTCCT GGCGCGTACG ACCCGATGAT 
GTCCTCATCG AGATGGGCGG CATGTTTGGT TCGAAGGGAG GCTTGCAGCG CCTGGATGTG 
GAGAACATAT CGCTGCAGCA GTTCGGTATC CATTCGGGAC GCGCATCGAT AGCCAGCTTC 
ACGTCGCTGC CGCCGCAAGT GTACACCACC ATTGGCCAGT TCAAGGGCGA ACGGGTGGCC 
ATCAAGAAGG TGAACGTGAA GAAGGTGGAC CTGACGCCGC AGCTCCTTTG GGAGATCAAG 
CAGGCGCGGG ATGTGAGCCA CGAGAATACG GTGCGTTTCG TGGGCGCCTG CATCGATCTG 
CCGCGACCCA CCGTGCTCAT CCTCACCGAA TATTGCTCGA GGGGCAGTCT GAAGGATGTG 
CTGGAGAACG AGGCCATCGA GTTGGACTGG AACTTTCGCA TGTCTCTCAT CCACGACATT 
GTCAAGGGCA TGAACTATCT GCACAATAGC GATGTGGCTG CCCATGGAAA GCTGAGGTCT 
TGCAATTGCC TGATAGACGG ACGATTCGTT CTCAAGATCT CCGACTTTGG ACTGAGAACG 
CTGACCACGC CCTCTGATTT TGTGCGGGAT CAGAACTACT ACCTCAAGCT CTTGTGGATT 
GCTCCCGAAC TGCTTCCACT GACCACCATA CCTGGTTGCT GTCCAGCCAC GCAGCGCGGC 
GATGTGTACT CCTTTGGCAT TATTCTGGAG GAGATCGTCA ACCGTGGTGG ACCCTACCAG 
GAGGCGCGCC AGCAGATGGA CGTTCACACG ATCCTGCACA AGGTGCGCCA GTGCAATGGA 
TTCCGTCCGC TCATCAGAGA ACGCGAGTGT CCACCTGATC TGCTGGAGCT AATGGAGAAG 
TGCTGGGCAG ACAATCAGGA GGAGCGGCCC ACTTTCTCCA CCATACGCAG TAATATTCGC 
ACCATAATGA AAGGCTTCTG TGAGAATCTA ATGGATGATC TACTGAATCG CATGGAGCAG 
TATGCCAACA ATCTCGAGAG TCTCGTGGAG GAGAAAACCC GCCAGTTGAG CCTGGAAAAG 
CAGCGTACGG AGGAGCTGCT CTACCAAATC CTGCCGCGTC CTGTGGCCCA GCAACTGATG 
GCCGGCGATC TTGTGGAGCC GGAGGAGTTC AGCAGCGTGA CCATATACTT TAGTGACATC 
GTTGGCTTCA CCGAACTGTG CGCCCGTAGC AGTCCCATGG ACGTGGTCAA CTTCCTGAAT 
GACTTGTATA GCACATTTGA CCGAATCATT GGCTTCTACG ACGTCTACAA AGTGGAAACG 
ATTGGTGATG CCTATCTGGT GGTTTCTGGA TTGCCGGAGC CCAATGGTGA CAAGCATGCC 
CGCGAGATTG CTCTAATGGC CCTGGACATC CTACGAGCAG TCAGCTCCTT CAATCTCCGC 
CATAAACCGG AATACAAGAT ACAGATTCGC ATTGGGATGC ATTCGGGTTC CGTGTGCGCC 
GGAGTCGTGG GCAAGAAGAT GCCGCACTAC TGTTTGTTTG GCGACACGGT CAACACGGCG 
TCGAGAATGG AGAGCACTGG CCAGCCGGGC AAGATACATG TGTCCTCGGC CACCAAGGCC 
ATCCTGGACA AGTTCGGCAC CTTCCAAATG GAGCAGCGCG GCGATGTCGA GCTAAAGGGC 
AAGGGCACGG TCACCACCTA CTGGCTGAAT AGCACCTCCG AGGGCGAGGC GCGTCCTCCG 
ACTCCGCAGA TCCTGACCAC CGATGAGGTG CCCTTCCCGC TGCTCTTCGC CGGCATGGGA 
AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726013.2
CDS	1..3486
Translation	MHLLGISIHFFFLMYVNCFSAHPNPRRNDITWDDLNKDISLDSTTSLAGLNASDAGLEQRMYERSRESKSTQLSRYTEVGEMGSTMRVYNVGVLMASHLDSPFDLERCGPAVDLALDEINKVFLKPHNITLLKKKGSYPSCSGARAPGLAADMYFQDDVIAFIGPACAFALEPVARLAAYWNKPIITGMGDQPPSSEGELTVTSGILGRIHKWKNENTGMFKDKSKYPTLTRMSYCQCRLILVFASVIRQFNWNHVALLVDRSELFSWTVGKNLEYGLRQEGLLSFVKELNGNEEEVYENYLKDASMYARVVILSVRGVLVRKFMLAAHSLGMTNGEWVFLDVEIFQSEYWGDKGWEMKDEHDAKARKAYEALLRVSLLQPTSPKFQDFADNVRENALYDYNYTFGEGEEVNFFIGAFYDGVYLLGMALNETLTEGGDIRDGVNITRRMWNRTFEGITGHVRIDDNGDRDADYSILDLDPINGKFSVVAHYSGVHKVYSAVHGKKIHWPGGREEPPPDVPPCGFLGNSTDCVGNFSTIMYSVFGLLLFLGLVFLVICLLQKQMKLSKELNNMSWRVRPDDVLIEMGGMFGSKGGLQRLDVENISLQQFGIHSGRASIASFTSLPPQVYTTIGQFKGERVAIKKVNVKKVDLTPQLLWEIKQARDVSHENTVRFVGACIDLPRPTVLILTEYCSRGSLKDVLENEAIELDWNFRMSLIHDIVKGMNYLHNSDVAAHGKLRSCNCLIDGRFVLKISDFGLRTLTTPSDFVRDQNYYLKLLWIAPELLPLTTIPGCCPATQRGDVYSFGIILEEIVNRGGPYQEARQQMDVHTILHKVRQCNGFRPLIRERECPPDLLELMEKCWADNQEERPTFSTIRSNIRTIMKGFCENLMDDLLNRMEQYANNLESLVEEKTRQLSLEKQRTEELLYQILPRPVAQQLMAGDLVEPEEFSSVTIYFSDIVGFTELCARSSPMDVVNFLNDLYSTFDRIIGFYDVYKVETIGDAYLVVSGLPEPNGDKHAREIALMALDILRAVSSFNLRHKPEYKIQIRIGMHSGSVCAGVVGKKMPHYCLFGDTVNTASRMESTGQPGKIHVSSATKAILDKFGTFQMEQRGDVELKGKGTVTTYWLNSTSEGEARPPTPQILTTDEVPFPLLFAGMGK

Target ORF information:

RefSeq Version	NM_166415.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG3216), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166415.2

ORF Insert Sequence:

ATGCATCTGC TTGGGATCAG CATCCACTTC TTCTTCCTGA TGTACGTCAA CTGCTTTAGT 
GCCCATCCGA ATCCCAGGCG CAATGATATA ACCTGGGATG ATCTGAACAA GGATATTAGT 
TTGGACTCCA CTACTTCCTT GGCGGGGCTC AATGCCAGTG ATGCGGGCCT AGAGCAAAGA 
ATGTACGAGA GGAGCAGGGA ATCCAAGTCT ACGCAGCTTT CTAGATACAC TGAGGTGGGC 
GAAATGGGCT CCACAATGCG TGTCTATAAC GTTGGCGTTC TGATGGCTTC TCATTTGGAT 
TCTCCATTCG ATCTGGAGCG TTGTGGACCG GCCGTTGACT TGGCCTTGGA TGAGATTAAC 
AAGGTTTTCC TGAAGCCTCA CAACATAACA CTACTGAAAA AGAAGGGAAG CTATCCATCC 
TGTTCGGGTG CTCGGGCTCC TGGACTGGCT GCGGACATGT ACTTCCAGGA CGACGTAATC 
GCGTTTATTG GACCAGCCTG CGCCTTTGCC CTTGAGCCGG TGGCACGTTT GGCCGCCTAT 
TGGAACAAGC CCATCATCAC CGGCATGGGA GATCAGCCGC CTTCCTCGGA AGGTGAGCTG 
ACGGTCACTT CCGGAATCCT GGGAAGGATA CACAAGTGGA AGAATGAGAA TACGGGCATG 
TTCAAGGACA AGTCCAAGTA CCCGACGCTC ACCCGGATGT CCTACTGCCA GTGCCGCCTC 
ATCCTGGTCT TTGCCAGCGT CATTCGGCAG TTCAACTGGA ATCACGTTGC CCTGCTGGTG 
GACAGATCGG AGCTCTTCTC GTGGACGGTG GGCAAGAATC TGGAGTACGG TCTGCGGCAG 
GAGGGACTCT TGAGCTTCGT CAAGGAGCTC AACGGCAACG AGGAGGAGGT GTACGAAAAC 
TATCTAAAGG ACGCCAGCAT GTATGCCAGA GTGGTCATCC TGTCGGTTCG CGGCGTTCTT 
GTGCGAAAAT TCATGTTGGC AGCCCACAGC CTGGGCATGA CCAATGGCGA GTGGGTGTTC 
CTGGACGTGG AAATCTTTCA GAGCGAATAC TGGGGCGACA AGGGTTGGGA GATGAAGGAT 
GAGCACGATG CCAAGGCGCG GAAGGCATAC GAAGCCCTCC TGAGAGTCAG TCTCCTGCAG 
CCGACAAGTC CCAAGTTCCA GGACTTTGCC GACAATGTGA GGGAGAATGC GCTCTACGAT 
TACAACTACA CGTTTGGCGA GGGCGAGGAG GTGAACTTCT TTATTGGAGC CTTCTACGAT 
GGCGTCTACC TGCTGGGAAT GGCCTTGAAC GAGACCCTCA CCGAGGGTGG TGACATCCGG 
GATGGGGTCA ACATCACCAG GCGGATGTGG AATCGCACCT TCGAAGGTAT CACGGGGCAC 
GTGCGCATCG ACGACAACGG TGACCGGGAT GCTGATTACT CCATTCTCGA TCTGGATCCC 
ATCAATGGAA AGTTCTCCGT TGTGGCCCAT TATTCCGGTG TTCACAAAGT ATATTCGGCT 
GTTCATGGCA AGAAGATACA TTGGCCTGGT GGTCGTGAGG AGCCACCGCC GGATGTGCCA 
CCCTGTGGCT TCCTGGGCAA TTCTACAGAC TGTGTCGGAA ACTTTTCTAC CATTATGTAC 
TCCGTGTTTG GATTGCTTCT TTTTCTGGGC CTTGTTTTTC TGGTCATCTG TCTTCTGCAA 
AAGCAGATGA AGCTCAGCAA GGAGCTGAAT AATATGTCCT GGCGCGTACG ACCCGATGAT 
GTCCTCATCG AGATGGGCGG CATGTTTGGT TCGAAGGGAG GCTTGCAGCG CCTGGATGTG 
GAGAACATAT CGCTGCAGCA GTTCGGTATC CATTCGGGAC GCGCATCGAT AGCCAGCTTC 
ACGTCGCTGC CGCCGCAAGT GTACACCACC ATTGGCCAGT TCAAGGGCGA ACGGGTGGCC 
ATCAAGAAGG TGAACGTGAA GAAGGTGGAC CTGACGCCGC AGCTCCTTTG GGAGATCAAG 
CAGGCGCGGG ATGTGAGCCA CGAGAATACG GTGCGTTTCG TGGGCGCCTG CATCGATCTG 
CCGCGACCCA CCGTGCTCAT CCTCACCGAA TATTGCTCGA GGGGCAGTCT GAAGGATGTG 
CTGGAGAACG AGGCCATCGA GTTGGACTGG AACTTTCGCA TGTCTCTCAT CCACGACATT 
GTCAAGGGCA TGAACTATCT GCACAATAGC GATGTGGCTG CCCATGGAAA GCTGAGGTCT 
TGCAATTGCC TGATAGACGG ACGATTCGTT CTCAAGATCT CCGACTTTGG ACTGAGAACG 
CTGACCACGC CCTCTGATTT TGTGCGGGAT CAGAACTACT ACCTCAAGCT CTTGTGGATT 
GCTCCCGAAC TGCTTCCACT GACCACCATA CCTGGTTGCT GTCCAGCCAC GCAGCGCGGC 
GATGTGTACT CCTTTGGCAT TATTCTGGAG GAGATCGTCA ACCGTGGTGG ACCCTACCAG 
GAGGCGCGCC AGCAGATGGA CGTTCACACG ATCCTGCACA AGGTGCGCCA GTGCAATGGA 
TTCCGTCCGC TCATCAGAGA ACGCGAGTGT CCACCTGATC TGCTGGAGCT AATGGAGAAG 
TGCTGGGCAG ACAATCAGGA GGAGCGGCCC ACTTTCTCCA CCATACGCAG TAATATTCGC 
ACCATAATGA AAGGCTTCTG TGAGAATCTA ATGGATGATC TACTGAATCG CATGGAGCAG 
TATGCCAACA ATCTCGAGAG TCTCGTGGAG GAGAAAACCC GCCAGTTGAG CCTGGAAAAG 
CAGCGTACGG AGGAGCTGCT CTACCAAATC CTGCCGCGTC CTGTGGCCCA GCAACTGATG 
GCCGGCGATC TTGTGGAGCC GGAGGAGTTC AGCAGCGTGA CCATATACTT TAGTGACATC 
GTTGGCTTCA CCGAACTGTG CGCCCGTAGC AGTCCCATGG ACGTGGTCAA CTTCCTGAAT 
GACTTGTATA GCACATTTGA CCGAATCATT GGCTTCTACG ACGTCTACAA AGTGGAAACG 
ATTGGTGATG CCTATCTGGT GGTTTCTGGA TTGCCGGAGC CCAATGGTGA CAAGCATGCC 
CGCGAGATTG CTCTAATGGC CCTGGACATC CTACGAGCAG TCAGCTCCTT CAATCTCCGC 
CATAAACCGG AATACAAGAT ACAGATTCGC ATTGGGATGC ATTCGGGTTC CGTGTGCGCC 
GGAGTCGTGG GCAAGAAGAT GCCGCACTAC TGTTTGTTTG GCGACACGGT CAACACGGCG 
TCGAGAATGG AGAGCACTGG CCAGCCGGGC AAGATACATG TGTCCTCGGC CACCAAGGCC 
ATCCTGGACA AGTTCGGCAC CTTCCAAATG GAGCAGCGCG GCGATGTCGA GCTAAAGGGC 
AAGGGCACGG TCACCACCTA CTGGCTGAAT AGCACCTCCG AGGGCGAGGC GCGTCCTCCG 
ACTCCGCAGA TCCTGACCAC CGATGAGGTG CCCTTCCCGC TGCTCTTCGC CGGCATGGGA 
AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG3216 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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